Amita Verma | Sam Higgin Bottom Institute Of Agriculture Sciences And Technology (original) (raw)

Papers by Amita Verma

Background: Chronic suppurative otitis media (CSOM) is defined as a persistent infection of the m... more Background: Chronic suppurative otitis media (CSOM) is defined as a persistent infection of the middle ear with a perforated tympanic membrane and draining exudate for more than 6 weeks. Information about the organism responsible for COSM and their antibiotic susceptibility pattern is an important for effective treatment. Aim: This study aims to develop protocol for empirical treatment by determining aerobic bacterial profile and antibiotics susceptibility in patients of chronic suppurative otitis media (CSOM) at a secondary care hospital in North India. Material and Methods: A cross-sectional study was conducted at ENT department of the secondary care hospital in North India on 85 patients, middle ear discharge sample was collected under strict aseptic conditions and antibiotic susceptibility done as per Clinical Laboratory Standards Institute guidelines. Result: 85 ear swabs were collected, and 89 bacterial isolates were identified, of which 62 (72.94%) sample with mono-microbial ...

Pharmacognosy Journal, 2011

amaranthine and isoamaranthine, gomphrenin, betanin, b-sitosterol, stigmasterol, linoleic acid, 0... more amaranthine and isoamaranthine, gomphrenin, betanin, b-sitosterol, stigmasterol, linoleic acid, 0.15% rutin and beta-carotene. [7-8] The present investigation deals with the study of some pharmacognostical characteristic of the leaves as a whole and in their powdered form. MATERIAL AND METHODS Collection and authentication of plant material The plant material was collected from the National Botanical Research Institute Garden, Lucknow and identified by a Taxonomist Dr. AKS Rawat with identification number CIF-RB-2-126-1. The leaves were separated, dried, coarsely powdered passed through sieve no 40 and stored in a closed container for further use. All reagents used were of analytical grade. Morphological and Microscopical analysis The macroscopical characters (size, shape colour, odour, texture, margin, base, apex and petiole) of the leaves were observed. Powder was identified with routine reagents to study the lignified cells, trichomes, stomata, fibers etc. Quantitative microscopy like measurement of Stomatal number, Stomatal index, Vein islet number, Vein termination

Coronaviruses

Background: In December 2019, a large number of patients with a novel coronavirus were identified... more Background: In December 2019, a large number of patients with a novel coronavirus were identified in Wuhan, China. The novel coronavirus (COVID-19) is highly contagious and increasing the rate of mortality day by day. The World Health Organization declared COVID-19 as a worldwide pandemic on March 11, 2020. Early diagnosis, of SARS-CoV-2, can restrict the COVID-19 pandemic. Objective: We aim to study the currently available diagnostic methods for COVID-19. Methods: World Health Organisation portal, Centre for Disease control and prevention portal, Indian Council of Medical Research portal, Chinese Centre for Disease Control and prevention portal, Science Direct, Google Scholar, Research Gate, etc. were searched for obtaining data. Results: Rapid diagnosis and prompt treatment can reduce the number of prospective cases. The diagnostic strategy encompasses the screening of virus with nucleic acid amplification test (NAAT) such as real-time reverse-transcription polymerase chain reacti...

Journal of Pharmaceutical Research

The objective of the study was to investigate the possible effects of 50% ethanolic extract of Ca... more The objective of the study was to investigate the possible effects of 50% ethanolic extract of Capparis zeylanica (CZE) on gastric secretion and Phenylbutazone ulcer in rats. Approach: Twenty four adult male rats were used in this study (four groups) and several parameters were determined to assess any degree of protection. The ulceration was induced by Phenylbutazone at a dose of 100 mg/kg b.w. Findings: Phenylbutazone toxicity induced + CZE treated rats showed a significant decrease (p < 0.001) in volume of gastric juice, free and total acidity, pepsin concentration and acid output with a significant increase (p < 0.001) in pH compared to Phenylbutazone toxicity induced rats. Phenylbutazone caused a significant decrease (p < 0.001) in TC:P with a concomitant increase (p < 0.001) in protein and DNA. After treatment of CZE in rats, a significant increase (p < 0.001) in the carbohydrate concentration in terms of TC:P ratio and significantly lowered (p < 0.001) protein concentration in gastric juice was observed. CZE tended to increase mucin activity in Phenylbutazone + CZE group. Conclusion: It can be concluded that CZE imparted a protective action against phenylbutazone induced chronic ulcer in rats.

RSC Advances

Wedelia calendulacea has a long history of use in the Indian Ayurvedic System of Medicine for the... more Wedelia calendulacea has a long history of use in the Indian Ayurvedic System of Medicine for the treatment, prevention, and cure of a diverse range of human diseases such as diabetes obesity, and other metabolic diseases.

Recent patents on anti-infective drug discovery, Jan 13, 2018

There is the need of development of liposomes based nanomedicines formulation for the better effi... more There is the need of development of liposomes based nanomedicines formulation for the better efficacy and safety of the available drugs in the market. Liposomes have various applications in the field of pharmaceutical and medical field for their drug target potential, diagnostic importance and imaging techniques. Natural plant based drugs and their derivative have been used in the medicine, nutraceuticals, perfumery, cosmetic and beverages industry. More than half of the prescribed drugs in the worldwide are mainly derived from different natural sources. Development of plant derived product is an emerging field of food, pharmaceutical and health industries. Plants belonging to Gentianaecae family are well known for their bitter taste and Swertia chirata is one of best plant among them. Various active phytochemical of Swertia chirata are bitter secoiridoids like gentiopicroside, amarogentin, swertiamarin, isovitexin and isogentisin. Peoples are using different species of Swertia in t...

$Research paper thumbnail of {"__content__"=>"One-pot green synthesis and structural characterisation of silver nanoparticles using aqueous leaves extract of : antioxidant, anticancer and antibacterial activities.", "i"=>{"__content__"=>"Carissa carandas"}}$

IET nanobiotechnology, 2018

Facile green synthesis of silver nanoparticles (AgNPs) using an aqueous extract of () leaves was ... more Facile green synthesis of silver nanoparticles (AgNPs) using an aqueous extract of () leaves was studied. Fabrication of AgNPs was confirmed by the UV-visible spectroscopy which gives absorption maxima at 420 nm. C. carandas leaves are the rich source of the bioactive molecules, acts as a reducing and stabilising agent in AgNPs, confirmed by Fourier transforms infrared spectroscopy. The field emission scanning electron microscope revealed the spherical shape of biosynthesised AgNPs. A distinctive peak of silver at 3 keV was determined by energy dispersive X-ray spectroscopy. X-ray diffraction showed the facecentred cubic structure of biosynthesised AgNPs and thermal stability was confirmed by the thermogravimetric analysis. Total flavonoid and total phenolic contents were evaluated in biosynthesised AgNPs. Biosynthesised AgNPs showed free radical scavenging activities against 2, 2-diphenyl-1-picrylhydrazyl test and ferric reducing antioxidant power assay. cytotoxicity against hepati...

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Jan 2, 2018

Citrous lemon (Rutaceae) an Indian folk medicine has been used for the treatment of various patho... more Citrous lemon (Rutaceae) an Indian folk medicine has been used for the treatment of various pathological diseases viz., diabetes, cardiovascular, inflammation, hepatobiliary dysfunction and neurodegenerative disorder. Can lemon oil altered the memory of unstressed and stressed mice, a basic question for which the present work was put on trial. The present investigation was intended to assess the impact of Lemon oil on memory of unstressed and Stressed Swiss young Albino mice. Lemon oil (50 and 100 mg/kg o.r.) and donepezil (10 mg/kg) were guided for three weeks to different groups of stressed and unstressed mice. The nootropic movement was assessed utilizing elevated plus maze and Hebbs Williams Maze. Cerebrum acetylcholinesterase (AChE), plasmacorticosterone, decreased glutathione, lipid per oxidation alongside superoxide dismutase and catalase was surveyed as marker for disease. Histopathology was performed for estimation of drug effects. Acute immobilized stress was induce, lemon...

Inflammopharmacology, Jan 16, 2018

A series of quinazoline clubbed 1,3,5-triazine derivatives (QCT) were synthesized and evaluated f... more A series of quinazoline clubbed 1,3,5-triazine derivatives (QCT) were synthesized and evaluated for their in vitro anticancer activity against HeLa (human cervical cancer), MCF-7 (human breast cancer cell), HL-60 (human promyelocytic leukemia cell), HepG2 (human Hepatocellular carcinoma cell), and one normal cell line HFF (human foreskin fibroblasts). In vitro assay result encouraged to further move towards in ovo anticancer evaluation using chick embryo. The series of QCT derivatives showed higher anticancer and antiangiogenic activity against HeLa and MCF-7 cell lines. In the series, synthetic molecule 8d, 8l, and 8m displayed significant activity. Further, these results substantiated by docking study on VGFR2. SAR study concluded that the potency of drugs depends on the nature of aliphatic substitution and the heterocyclic ring system.

International journal of nanomedicine, 2017

According to the World Health Organization, globally there are around 18 million patients sufferi... more According to the World Health Organization, globally there are around 18 million patients suffering from Alzheimer's disease (AD), and this number is expected to double by 2025. The pathophysiology of AD includes selective deposition of Aβ peptide in the mitochondria of cells, which inhibits uptake of glucose by neurons and key enzyme functions. Current drug treatments for AD are unable to rectify the underlying pathology of the disease; they only provide short-term symptomatic relief, so there is a need for the development of newer treatment regimes. The antiamyloid activity, antifibrinolytic activity, and antithrombotic activity of nattokinase holds potential for the treatment of AD. As nattokinase is a protein, its stability restricts its usage to a greater extent, but this limitation can be overcome by nanoencapsulation. In this work, we successfully synthesized polymeric nanoparticles of nattokinase and characterized its use by different techniques: transmission electron mi...

Journal of Coastal Life Medicine

Coccinia indica (Ivy Gourd), Antidiabetic activity, Antibacterial activity Traditional system of ... more Coccinia indica (Ivy Gourd), Antidiabetic activity, Antibacterial activity Traditional system of medicine consists of large number of plants with various medicinal and pharmacological importances and hence represents a priceless tank of new bioactive molecules. Coccinia indica belongs to the family Cucurbitaceae. It is a rapidly growing, perennial climber or trailing vine. Traditionally different parts of this plant namely the roots, leaves and fruits are used in folklore medicine for several purposes like jaundice, diabetes, wound healing, ulcers, stomach ache, skin disease, fever, asthma, cough. The leaf and its constituents have been reported to possess hypoglycaemic, hypolipidemic and antioxidant properties. This review provides adequate information to develop suitable therapeutics out of these plant parts.

Archives of physiology and biochemistry, Jan 24, 2017

Prunus amygdalus Batsch (almond) is a classical nutritive traditional Indian medicine. Along with... more Prunus amygdalus Batsch (almond) is a classical nutritive traditional Indian medicine. Along with nutritive with anti-oxidant properties, it is, clinically, used in the treatment of various diseases with underlying anti-oxidant mechanism. This study is an effort to scrutinise the renal protective effect of P. amygdalus Batsch or green almond (GA) seed coat extract and its underlying mechanism in animal model of Ferric nitrilotriacetate (Fe-NTA) induced renal cell carcinoma (RCC). RCC was induced in Swiss Albino Wistar rats by intraperitoneal injection of Fe-NTA. The rats were then treated with ethanolic extract of GA (25, 50 and 100 mg/kg per oral) for 22 weeks. Efficacy of GA administration was evaluated by change in biochemical, renal, macroscopical and histopathological parameters and alterations. Additionally, interleukin-6 (IL-6), tumour necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and inflammatory mediator including prostaglandin E2 (PGE2), nuclear factor-kappa B (NF-κB) ...

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Jan 9, 2017

It is well documented that anomalous production of inflammatory proteins linked with most of the ... more It is well documented that anomalous production of inflammatory proteins linked with most of the toxic expression and genesis of diverse chronic disease including cancer. Diethylnitrosamine (DEN) a well-known hepatotoxin and hepatocarcinogen, can induce oxidative stress and inflammatory reaction in it. Umbelliferone, secondary metabolites, is present in different plants and widely consumed by humans as medicine and food supplements. The aim of the current study was to scrutinize the chemoprotective potential of umbelliferon-α-d-glucopyranosyl-(2(I)→1(II))-α-d-glucopyranoside (UFD) against DEN-induced hepatocellular carcinoma (HCC) in experimental rats. Single intraperitoneal injection of DEN (200mg/kg) was used for induction of HCC in rats and rats were grouped and orally treated with UFD (5, 10 and 20mg/kg) dose for 22 weeks. Parameters under investigation included hepatic, non-hepatic enzymes, oxidative stress, pro-inflammatory cytokines, COX-2 and NF-κB level along with histopath...

Scientific reports, Jan 19, 2017

A novel series of hybrid analogues of monastrol-1,3,5-triazine were designed and developed via on... more A novel series of hybrid analogues of monastrol-1,3,5-triazine were designed and developed via one-pot synthesis using Bi(NO3)3 as a catalyst. Entire compounds were evaluated for their anticancer activity against HeLa (cervical cancer), MCF-7 (breast cancer), HL-60 (Human promyelocytic leukemia), HepG2 (Hepatocellular carcinoma) and MCF 12A (normal epithelial breast cell line) using MTT assay, where they showed highest inhibitory activity against MCF-7. The molecules were also found to be non-toxic to MCF 12A cells. These molecules showed considerable inhibitory percentage against Epidermal Growth Factor Receptor tyrosine kinase (EGFR-TK), in in-vitro assay. Molecular docking study was carried out on the analogs and reference compound (Erlotinib) into the ATP binding site of EGFR-TK domain (PDB ID:1M17) to elucidate vital structural residues necessary for bioactivity. The effect of most active compound 7l was also estimated in-vivo in DMBA induced mammary tumor in female Sprague-Daw...

Inflammopharmacology, Jan 12, 2017

The aerial part of Wedelia calendulacea have been used in Ayurveda, Unani, Tibetan, Siddha and ot... more The aerial part of Wedelia calendulacea have been used in Ayurveda, Unani, Tibetan, Siddha and other folk medicine systems to protect the liver and renal tissue. Liver is considered as primary metabolizing site of body, which is prone to damage by endogenous and exogenous toxicants. A reason for liver toxicity, and major causes of the hepatocellular carcinoma (HCC). 19-α-Hydroxyurs-12(13)-ene-28 oic acid-3-O-β-D-glucopyranoside (HEG), a triterpenoids found in the higher plants, has been known to possess protective effect against various toxicants. The aim of the current study was to scrutinize the hepatoprotective mechanism of HEG against DEN-induced oxidative stress, hyperproliferation, inflammation and apoptosis tissue injury in Wistar rats. Invitro cell lines study of HEG scrutinized against the Hep-G2 and HuH-7 cells. A single dose of DEN (200 mg/kg) and double dose of phenobarbitol were administered to induce the liver damage in rats; the dose treatment of HEG was terminated at...

Current drug targets, Jan 14, 2017

Immune-based inflammatory diseases are immune related dysregulation in different sites of body, w... more Immune-based inflammatory diseases are immune related dysregulation in different sites of body, which includes rheumatoid arthritis, multiple sclerosis, inflammatory bowel disease, atherosclerosis, etc. Advancements in molecular research have facilitated investigation of their pathogenesis which is involved with inflammatory cytokines cells and several genes. The available drug therapy provides suboptimal therapeutic effects and higher adverse effects. Emergence of liposomal systems of the drugs meant for the above mentioned disease have gained wider importance due to in their high treatment efficacy by means of optimal therapeutic drug delivery. Beyond the conventional liposomal formulations, evolution of second generation liposomes including stealth liposomes, cationic liposomes, immuno-liposomes, etc. have gained tremendous potential owing to their drug target potential, diagnostic importance and imaging in treatment of above mentioned immune mediated inflammatory disorders.

Inflammopharmacology, 2017

A new compound derivative of glycoside 19-α-hydroxy-ursolic acid glucoside (19-α-hydroxyurs-12(13... more A new compound derivative of glycoside 19-α-hydroxy-ursolic acid glucoside (19-α-hydroxyurs-12(13)-ene-28-oic acid-3-O-β-D-glucopyranoside (HEG) was isolated from whole plant of Wedelia calendulacea (Compositae). The structure was elucidated and established by standard spectroscopy approaches. Diethylnitrosamine (DEN) (200 mg/kg) and ferric nitrilotriacetate (Fe-NTA) (9 mg/kg) were used for induction of renal cell carcinoma (RCC) in the rats. The rats were further divided into different groups and were treated with HEG doses for 22 weeks. Anti-cancer effect in RCC by HEG was dose dependent to restrict the macroscopical changes as compared to DEN + Fe-NTA-control animals. Significant alteration in biochemical parameters and dose-dependent alleviation in Phase I and Phase II antioxidant enzymes were responsible for its chemo-protective nature. HEG in dose-dependent manner was significant to alter the elevated levels of pro-inflammatory cytokines and inflammatory mediators during RCC. ...

The Journal of pharmacy and pharmacology, Jan 24, 2016

The present review explores the therapeutic application of herbals in rheumatoid arthritis (RA) t... more The present review explores the therapeutic application of herbals in rheumatoid arthritis (RA) therapy, and how nano/submicromedicine can be fit in the scope of its therapeutic delivery in RA has been addressed. Incorporation of bioactive such as polyphenols, thymoquinone, resveratrol, hesperidin, curcumin, celastrol and gambogic acid in a dose-dependent manner showed quite high efficacy for the treatment of RA. It can be attributed to their targeting ability against various inflammatory mediators including nitric oxide (NO), cytokines, chemokines, adhesion molecules, NF-kβ, lipoxygenase (LOXs) and arachidonic acid (AA). Despite the presence of significant merits, the use of these bioactives has several demerits such as poor bioavailability as a function of low aqueous solubility and higher first-pass metabolism upon oral administration. The impact of nano/submicromedicine in the delivery of these bioactives against RA has gained wider attention owing to bioavailability enhancement...

Inflammopharmacology, 2016

Melastoma malabathricum Linn (MM) has high valued for its commercial significance. Indian market ... more Melastoma malabathricum Linn (MM) has high valued for its commercial significance. Indian market (northeast) has great demand for the plants, which extended, its use as a traditional home remedy due to its anti-inflammatory effects. In this study, we scrutinize the therapeutic and protective effect of MM against diethylnitrosamine (DEN) and ferric nitrilotriacetate (Fe-NTA)-induced renal carcinogenesis, renal hyperproliferation, and oxidative stress in rats. Liquid chromatography mass spectroscopy (LC-MS) was used for identification of phytoconstituents. Administration of DEN confirmed the initiation the renal carcinogenesis via enhancing the expansion of tumor incidence. Intraperitoneally, administration of Fe-NTA boost the antioxidant enzymes (phase I), viz., superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx) and phase II, viz., quinone reductase (QR) and glutathione-S-transferase (GST). It also increased the content of renal lipid...