Ramesh Raj Puri | Shimane University (original) (raw)
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Papers by Ramesh Raj Puri
Exploring stress tolerance indices to identify terminal heat tolerance in spring
This paper can be downloaded online at http://ijasbt.org & http://nepjol.info/index.php/IJASBT 65... more This paper can be downloaded online at http://ijasbt.org & http://nepjol.info/index.php/IJASBT 65 Variability, Correlation and Path Coefficient Analysis for Agro-morphological Traits in Wheat Genotypes (Triticum aestivum L.) under Normal and Heat Stress Conditions Mukti Ram Poudel , Padam Bahadur Poudel , Ramesh Raj Puri , Hema Kumari Paudel Paklihawa Campus, Institute of Agriculture and Animal Science, Tribhuvan University, Rupandehi, Nepal Department of Jobs, Precincts and Regions, Horsham, 3400, Victoria, Australia. Nepal Agriculture Research Council, Nepal
Colonization of a biofertilizer Bacillus sp. OYK strain, which was isolated from a soil, was comp... more Colonization of a biofertilizer Bacillus sp. OYK strain, which was isolated from a soil, was compared with three rhizospheric and endophytic Bacillus sp. strains to evaluate the colonization potential of the Bacillus sp. strains with a different origin. Surface-sterilized seeds of tomato (Solanum lycopersicum L. cv. Chika) were sown in the sterilized vermiculite, and four Bacillus sp. strains were each inoculated onto the seed zone. After cultivation in a phytotron, plant growth parameters and populations of the inoculants in the root, shoot, and rhizosphere were determined. In addition, effects of co-inoculation and time interval inoculation of Bacillus sp. F-33 with the other endophytes were examined. All Bacillus sp. strains promoted plant growth except for Bacillus sp. RF-37, and populations of the rhizospheric and endophytic Bacillus sp. strains were 1.4–2.8 orders higher in the tomato plant than that of Bacillus sp. OYK. The plant growth promotion by Bacillus sp. F-33 was redu...
Terminal heat stress during anthesis and grain filling period accelerates maturity and significan... more Terminal heat stress during anthesis and grain filling period accelerates maturity and significantly reduces grain size and weight. A study was performed to explore stress tolerance indices that can judge terminal heat tolerance in spring wheat in Nepal. Ten diverse lines were planted under normal (non-stress) and late (heat stress) sown condition at two locations (Parwanipur and Hardinath) in Nepal. The trial was set up in Alpha Lattice design with two replications in two consecutive crop cycles 2011-12 and 2012-13. Yield traits were recorded and stress susceptibility and tolerant indices were estimated. Correlation analysis showed that yield under stress environment had positive (r=0.80) and significant (p<0.05) association with that under non-stress environment. Similarly, grain yield under both environments had significant and positive correlation with mean productivity (MP), geometric mean productivity (GMP) and heat tolerance index (HTI) whereas non-significant correlation ...
Journal of Agriculture and Food Research
Asian Journal of Research in Agriculture and Forestry
High temperature stress unfavorably affects plant growth and reduces grain yield (GY). This study... more High temperature stress unfavorably affects plant growth and reduces grain yield (GY). This study was conducted with an aim to identify the terminal heat tolerance of one hundred and two wheat genotypes with three checks. They were sown under normal (non-stress) and late (stress) conditions at Regional Agricultural Research Station (RARS), Tarahara; RARS, Nepalgunj and National Wheat Research Program (NWRP), Bhairahawa, Nepal. The trial was sown in Augmented design during 2014/15 winter season as a Nepal heat tolerance wheat screening nursery (NHTWSN). Grain yield, maturity, stress susceptibility and tolerant indices were estimated to assess the heat tolerance of the genotypes. Combined analysis among the tested wheat lines (102 new entries + 3 checks) showed that KACHU//KIRITATI/WBLL1 ((Heat tolerance index (HTI) = 1.78) possessed the highest level of heat tolerance, followed by SLVS /3/ CROC_1/ AE.SQUARROSA (224)// OPATA/5/ VEE/LIRA//BOW/3/BCN/4/KAUZ/6/ 2*KA/NAC//TRCH (HTI=1.57) ...
World Journal of Microbiology and Biotechnology
The aim of this study was to clarify effects of soil and climatic conditions on community structu... more The aim of this study was to clarify effects of soil and climatic conditions on community structure of sweet potato bacterial endophytes by applying locked nucleic acid oligonucleotide-PCR clamping technique and metagenomic analysis. For this purpose, the soil samples in three locations were transferred each other and sweet potato nursery plants from the same farm were cultivated for ca. 3 months. After removal of plastid, mitochondria and undefined sequences, the averaged numbers of retained sequences and operational taxonomic units per sample were 20,891 and 846, respectively. Proteobacteria (85.0%), Bacteroidetes (6.6%) and Actinobacteria (6.3%) were the three most dominant phyla, accounting for 97.9% of the reads, and γ-Proteobacteria (66.3%) being the most abundant. Top 10 genera represented 81.2% of the overall reads in which Pseudomonas (31.9-45.0%) being the most predominant. The overall endophytic bacterial communities were similar among the samples which indicated that the soil and the climatic conditions did not considerably affect the entire endophytic community. The original endophytic bacterial community might be kept during the cultivation period.
Horticulturae
Endophytic bacteria form a symbiotic relation with plants and generally cause no harmful effects ... more Endophytic bacteria form a symbiotic relation with plants and generally cause no harmful effects to the host plants. In a previous study, we isolated eight bacterial endophytes from sweet potato plants harvested in Salyan, Nepal. These endophytes showed plant growth-promoting properties as a mixed culture. In this study, we evaluated the ability of these strains to produce indole-3-acetic acid (IAA) and to fix nitrogen. Based on these results, we selected two strains, Klebsiella sp. Sal 1 and Enterobacter sp. Sal 3, and evaluated their ability to promote plant growth. IAA production activity peaked at 15–60 mg NH4NO3/L in plant-free medium. Similarly, acetylene reduction activity peaked at 0–6.25 mg NH4NO3/L. Both strains successfully colonized plants, promoted the growth of tomatoes, and induced phenotypes in plants consistent with IAA exposure. This suggests that these strains promote plant growth by producing IAA inside the plant, where nitrogen levels are expected to be low.
International Journal of Environment, 2015
The study was conducted to evaluate phenotypic variation in one hundred and sixty six wheat landr... more The study was conducted to evaluate phenotypic variation in one hundred and sixty six wheat landraces from mid and far western districts of Nepal. They were sown in randomized complete block design with two replications at National Wheat Research Program in 2014/15. The observed traits were analyzed using descriptive statistics and multivariate analysis using MINITAB v. 14. The results showed a wide range of phenotypic variability in observed parameters. The results also showed that the highest value of the standard deviation from mean (Sd) was for grain yield (±290.10) followed by plant height (±7.21). Among the traits the lowest deviation from mean (Sd) was for thousand grain weight TGW (±2.68). Wheat landraces grouped in four clusters depending on similarity of the studied traits. The results in this cluster, showed that days to maturity ranged from 97 to111 days, TGW ranged from 16 to17 gm, plant height ranged from 76 to 85 cm, and grain yield ranged from 2800 to 3000 Kg ha-1. Wheat landraces under study are grouped depending on specific traits useful for wheat improvement program. Results of this study can be supportive to detect wheat landraces within species with similar traits. In addition it can be useful for sampling in successive studies and parental selection in wheat breeding program.
Taro can be found in wide agro-ecological domains stretching from plains (<100m) to high-hills... more Taro can be found in wide agro-ecological domains stretching from plains (<100m) to high-hills (2500m). In terai and mid-hill conditions, the crop is found in two distinct agro-ecosystems: first, commonly in home gardens and second, on newly cleared forestland or inter-cropped with ginger and maize. In order to assess the diversity in qualitative and quantitative characters linked to food traditions a focus group discussions and field observations (n=31) methodologies were followed. The study was conducted at Shivgunj VDC of Jhapa district, Nepal. The study comprised of 13 different species of taro from home gardens of Jhapa district. Farmers' rank for qualitative and quantitative characters, the cultivars formed 3 distinct clusters. Darshane and Chama Kachchu formed a cluster characterized by same plant height, irritation characters and no aroma. Similarly, Koili and Lanke formed distinct cluster characterized by high market price and good taste. On the basis of farmers'...
International Journal of Environment, 2015
FIELD BASED ASSESSMENT OF FOLIAR BLIGHTS DISEASE OF WHEAT (Triticum aestivum L.
International Journal of Environment, 2015
Terminal heat stress during anthesis and grain filling period accelerates maturity and significan... more Terminal heat stress during anthesis and grain filling period accelerates maturity and significantly reduces grain size and weight. A study was performed to explore stress tolerance indices that can judge terminal heat tolerance in spring wheat in Nepal. Ten diverse lines were planted under normal (non-stress) and late (heat stress) sown condition at two locations (Parwanipur and Hardinath) in Nepal. The trial was set up in Alpha Lattice design with two replications in two consecutive crop cycles 2011-12 and 2012-13. Yield traits were recorded and stress susceptibility and tolerant indices were estimated. Correlation analysis showed that yield under stress environment had positive (r=0.80) and significant (p<0.05) association with that under non-stress environment. Similarly, grain yield under both environments had significant and positive correlation with mean productivity (MP), geometric mean productivity (GMP) and heat tolerance index (HTI) whereas non-significant correlation ...
Agronomy Journal of Nepal, 2013
An experiment was conducted at the Agronomy farm of the Institute of Agriculture and Animal Scien... more An experiment was conducted at the Agronomy farm of the Institute of Agriculture and Animal Science (IAAS), Rampur, Nepal in 2008/09. The experiment was laid out in factorial design in spit plot arrangement consisting of date of sowing as main factor and wheat genotypes as sub factor. Wheat genotypes were sown in 22 November for normal sowing and 29 December for late sowing condition. These were assessed against Helminthosporium leaf blight and leaf rust severity in a naturally inoculated environment. Result showed that wheat genotypes differed significantly with respect to flag leaf area, flag leaf duration and plant height. Genotype BL-3064 showed the lower decline (624.9 to 615.1) in chlorophyll content whereas RR-21 showed highest decline (471.2 to 360.4) in chlorophyll content from normal to late sowing, possibly contributing to resistant to the disease. The AUDPC value revealed that genotypes BL-3625 and BL-3623 had increased level of resistance due to lower AUDPC value and hi...
Journal of Advances in Microbiology
Exploring stress tolerance indices to identify terminal heat tolerance in spring
This paper can be downloaded online at http://ijasbt.org & http://nepjol.info/index.php/IJASBT 65... more This paper can be downloaded online at http://ijasbt.org & http://nepjol.info/index.php/IJASBT 65 Variability, Correlation and Path Coefficient Analysis for Agro-morphological Traits in Wheat Genotypes (Triticum aestivum L.) under Normal and Heat Stress Conditions Mukti Ram Poudel , Padam Bahadur Poudel , Ramesh Raj Puri , Hema Kumari Paudel Paklihawa Campus, Institute of Agriculture and Animal Science, Tribhuvan University, Rupandehi, Nepal Department of Jobs, Precincts and Regions, Horsham, 3400, Victoria, Australia. Nepal Agriculture Research Council, Nepal
Colonization of a biofertilizer Bacillus sp. OYK strain, which was isolated from a soil, was comp... more Colonization of a biofertilizer Bacillus sp. OYK strain, which was isolated from a soil, was compared with three rhizospheric and endophytic Bacillus sp. strains to evaluate the colonization potential of the Bacillus sp. strains with a different origin. Surface-sterilized seeds of tomato (Solanum lycopersicum L. cv. Chika) were sown in the sterilized vermiculite, and four Bacillus sp. strains were each inoculated onto the seed zone. After cultivation in a phytotron, plant growth parameters and populations of the inoculants in the root, shoot, and rhizosphere were determined. In addition, effects of co-inoculation and time interval inoculation of Bacillus sp. F-33 with the other endophytes were examined. All Bacillus sp. strains promoted plant growth except for Bacillus sp. RF-37, and populations of the rhizospheric and endophytic Bacillus sp. strains were 1.4–2.8 orders higher in the tomato plant than that of Bacillus sp. OYK. The plant growth promotion by Bacillus sp. F-33 was redu...
Terminal heat stress during anthesis and grain filling period accelerates maturity and significan... more Terminal heat stress during anthesis and grain filling period accelerates maturity and significantly reduces grain size and weight. A study was performed to explore stress tolerance indices that can judge terminal heat tolerance in spring wheat in Nepal. Ten diverse lines were planted under normal (non-stress) and late (heat stress) sown condition at two locations (Parwanipur and Hardinath) in Nepal. The trial was set up in Alpha Lattice design with two replications in two consecutive crop cycles 2011-12 and 2012-13. Yield traits were recorded and stress susceptibility and tolerant indices were estimated. Correlation analysis showed that yield under stress environment had positive (r=0.80) and significant (p<0.05) association with that under non-stress environment. Similarly, grain yield under both environments had significant and positive correlation with mean productivity (MP), geometric mean productivity (GMP) and heat tolerance index (HTI) whereas non-significant correlation ...
Journal of Agriculture and Food Research
Asian Journal of Research in Agriculture and Forestry
High temperature stress unfavorably affects plant growth and reduces grain yield (GY). This study... more High temperature stress unfavorably affects plant growth and reduces grain yield (GY). This study was conducted with an aim to identify the terminal heat tolerance of one hundred and two wheat genotypes with three checks. They were sown under normal (non-stress) and late (stress) conditions at Regional Agricultural Research Station (RARS), Tarahara; RARS, Nepalgunj and National Wheat Research Program (NWRP), Bhairahawa, Nepal. The trial was sown in Augmented design during 2014/15 winter season as a Nepal heat tolerance wheat screening nursery (NHTWSN). Grain yield, maturity, stress susceptibility and tolerant indices were estimated to assess the heat tolerance of the genotypes. Combined analysis among the tested wheat lines (102 new entries + 3 checks) showed that KACHU//KIRITATI/WBLL1 ((Heat tolerance index (HTI) = 1.78) possessed the highest level of heat tolerance, followed by SLVS /3/ CROC_1/ AE.SQUARROSA (224)// OPATA/5/ VEE/LIRA//BOW/3/BCN/4/KAUZ/6/ 2*KA/NAC//TRCH (HTI=1.57) ...
World Journal of Microbiology and Biotechnology
The aim of this study was to clarify effects of soil and climatic conditions on community structu... more The aim of this study was to clarify effects of soil and climatic conditions on community structure of sweet potato bacterial endophytes by applying locked nucleic acid oligonucleotide-PCR clamping technique and metagenomic analysis. For this purpose, the soil samples in three locations were transferred each other and sweet potato nursery plants from the same farm were cultivated for ca. 3 months. After removal of plastid, mitochondria and undefined sequences, the averaged numbers of retained sequences and operational taxonomic units per sample were 20,891 and 846, respectively. Proteobacteria (85.0%), Bacteroidetes (6.6%) and Actinobacteria (6.3%) were the three most dominant phyla, accounting for 97.9% of the reads, and γ-Proteobacteria (66.3%) being the most abundant. Top 10 genera represented 81.2% of the overall reads in which Pseudomonas (31.9-45.0%) being the most predominant. The overall endophytic bacterial communities were similar among the samples which indicated that the soil and the climatic conditions did not considerably affect the entire endophytic community. The original endophytic bacterial community might be kept during the cultivation period.
Horticulturae
Endophytic bacteria form a symbiotic relation with plants and generally cause no harmful effects ... more Endophytic bacteria form a symbiotic relation with plants and generally cause no harmful effects to the host plants. In a previous study, we isolated eight bacterial endophytes from sweet potato plants harvested in Salyan, Nepal. These endophytes showed plant growth-promoting properties as a mixed culture. In this study, we evaluated the ability of these strains to produce indole-3-acetic acid (IAA) and to fix nitrogen. Based on these results, we selected two strains, Klebsiella sp. Sal 1 and Enterobacter sp. Sal 3, and evaluated their ability to promote plant growth. IAA production activity peaked at 15–60 mg NH4NO3/L in plant-free medium. Similarly, acetylene reduction activity peaked at 0–6.25 mg NH4NO3/L. Both strains successfully colonized plants, promoted the growth of tomatoes, and induced phenotypes in plants consistent with IAA exposure. This suggests that these strains promote plant growth by producing IAA inside the plant, where nitrogen levels are expected to be low.
International Journal of Environment, 2015
The study was conducted to evaluate phenotypic variation in one hundred and sixty six wheat landr... more The study was conducted to evaluate phenotypic variation in one hundred and sixty six wheat landraces from mid and far western districts of Nepal. They were sown in randomized complete block design with two replications at National Wheat Research Program in 2014/15. The observed traits were analyzed using descriptive statistics and multivariate analysis using MINITAB v. 14. The results showed a wide range of phenotypic variability in observed parameters. The results also showed that the highest value of the standard deviation from mean (Sd) was for grain yield (±290.10) followed by plant height (±7.21). Among the traits the lowest deviation from mean (Sd) was for thousand grain weight TGW (±2.68). Wheat landraces grouped in four clusters depending on similarity of the studied traits. The results in this cluster, showed that days to maturity ranged from 97 to111 days, TGW ranged from 16 to17 gm, plant height ranged from 76 to 85 cm, and grain yield ranged from 2800 to 3000 Kg ha-1. Wheat landraces under study are grouped depending on specific traits useful for wheat improvement program. Results of this study can be supportive to detect wheat landraces within species with similar traits. In addition it can be useful for sampling in successive studies and parental selection in wheat breeding program.
Taro can be found in wide agro-ecological domains stretching from plains (<100m) to high-hills... more Taro can be found in wide agro-ecological domains stretching from plains (<100m) to high-hills (2500m). In terai and mid-hill conditions, the crop is found in two distinct agro-ecosystems: first, commonly in home gardens and second, on newly cleared forestland or inter-cropped with ginger and maize. In order to assess the diversity in qualitative and quantitative characters linked to food traditions a focus group discussions and field observations (n=31) methodologies were followed. The study was conducted at Shivgunj VDC of Jhapa district, Nepal. The study comprised of 13 different species of taro from home gardens of Jhapa district. Farmers' rank for qualitative and quantitative characters, the cultivars formed 3 distinct clusters. Darshane and Chama Kachchu formed a cluster characterized by same plant height, irritation characters and no aroma. Similarly, Koili and Lanke formed distinct cluster characterized by high market price and good taste. On the basis of farmers'...
International Journal of Environment, 2015
FIELD BASED ASSESSMENT OF FOLIAR BLIGHTS DISEASE OF WHEAT (Triticum aestivum L.
International Journal of Environment, 2015
Terminal heat stress during anthesis and grain filling period accelerates maturity and significan... more Terminal heat stress during anthesis and grain filling period accelerates maturity and significantly reduces grain size and weight. A study was performed to explore stress tolerance indices that can judge terminal heat tolerance in spring wheat in Nepal. Ten diverse lines were planted under normal (non-stress) and late (heat stress) sown condition at two locations (Parwanipur and Hardinath) in Nepal. The trial was set up in Alpha Lattice design with two replications in two consecutive crop cycles 2011-12 and 2012-13. Yield traits were recorded and stress susceptibility and tolerant indices were estimated. Correlation analysis showed that yield under stress environment had positive (r=0.80) and significant (p<0.05) association with that under non-stress environment. Similarly, grain yield under both environments had significant and positive correlation with mean productivity (MP), geometric mean productivity (GMP) and heat tolerance index (HTI) whereas non-significant correlation ...
Agronomy Journal of Nepal, 2013
An experiment was conducted at the Agronomy farm of the Institute of Agriculture and Animal Scien... more An experiment was conducted at the Agronomy farm of the Institute of Agriculture and Animal Science (IAAS), Rampur, Nepal in 2008/09. The experiment was laid out in factorial design in spit plot arrangement consisting of date of sowing as main factor and wheat genotypes as sub factor. Wheat genotypes were sown in 22 November for normal sowing and 29 December for late sowing condition. These were assessed against Helminthosporium leaf blight and leaf rust severity in a naturally inoculated environment. Result showed that wheat genotypes differed significantly with respect to flag leaf area, flag leaf duration and plant height. Genotype BL-3064 showed the lower decline (624.9 to 615.1) in chlorophyll content whereas RR-21 showed highest decline (471.2 to 360.4) in chlorophyll content from normal to late sowing, possibly contributing to resistant to the disease. The AUDPC value revealed that genotypes BL-3625 and BL-3623 had increased level of resistance due to lower AUDPC value and hi...
Journal of Advances in Microbiology