Gholamali Jelodar | Shiraz University (original) (raw)
Papers by Gholamali Jelodar
Indian Journal of Medical Sciences, 2005
Many traditional treatments have been recommended in the alternative system of medicine for treat... more Many traditional treatments have been recommended in the alternative system of medicine for treatment of diabetes mellitus; however, the mechanism of most of the herbals used has not been defined. This study was carried out to clarify the effect of fenugreek, garlic and onion, recommended in Persian folklore medicine as beneficial in the treatment of diabetes, on blood glucose and their possible effect on pancreatic tissue. Diabetes mellitus was induced in 20 out of 25 adult male albino rats, using intraperitoneal injection of 185 mg/kg BW alloxan. The diabetic rats were divided into four groups, three of which were fed a diet containing 12.5% BW Allium sativum (garlic), Allium cepa (onion) or Trigonella foenum-graecum (fenugreek) for 15 days. The fourth group (positive control) received an ordinary diet. The remaining non-diabetic rats (negative control group) received neither alloxan nor the mentioned plants. Following consumption of plants, blood glucose was measured every day and on the last day the pancreas were removed and stained with H&E and Gomeri aldehyde fuchsin (GAF). Morphology of the pancreatic sections and the following morphometric factors were studied: volume density of B cells, volume density of islets, percent of B cells, number of islets per square millimeter, average area of islets and average volume density of B cell in whole pancreas. One-way Analysis of Variance (ANOVA) test and Duncan's multiple range tests were used to evaluate the data. The results of this study indicate that only garlic was able to reduce blood glucose significantly compared with the control group (P<0.05). In the control positive group all the mentioned morphometric factors were significantly changed in comparison with the control negative (normal health) group, but the same did not show significant change between treated and untreated diabetics.
Comparative Clinical Pathology
Application of antibiotics in livestock production has been a big concern in the recent years sin... more Application of antibiotics in livestock production has been a big concern in the recent years since they can have a vital role in the emergence of antibiotic resistant bacteria. Therefore, scientists and nutritionists have been searching for alternative agents to alter ruminal metabolism and improve feed efficiency and animal productivity. In this respect, the aim of the present study was to evaluate the effects of onion extract on leptin, ghrelin, total antioxidant capacity (TAC), and the performance of suckling lambs. Eighteen Torki Qashqai suckling lambs with 30 days of age were randomly divided into three groups, including (1) control group; basement diet without onion extract + sheep milk, (2) T150 group; basement diet containing 150 mg/kg onion extract + sheep milk, (3) T250 group; basement diet containing 250 mg/kg onion extract + sheep milk. The trial lasted for 60 days and the blood samples were taken on days 30 and 60 of the experiment. The results showed that the addition of onion extract led to an increase in the feed intake and daily weight in lambs. Moreover, onion extract was able to increase the level of ghrelin and reduce leptin hormone in the treatment groups (T150 and T250). In conclusion, it could be said that onion extract has a promising effect as a feed additive in ruminant nutrition and has the ability to improve feed intake in these animals. Moreover, onion extract has a positive effect on concentrations of leptin, ghrelin, TAC, and the performance in lambs.
Indian journal of experimental biology, 2007
Endometrium of GnRH treated group resembled with pregnant group and endometrial thickness in thes... more Endometrium of GnRH treated group resembled with pregnant group and endometrial thickness in these groups significantly increased in comparison with non-pregnant group. In GnRH treated animals, most of histomorphological changes in epithelial cells, glands and stroma of uterus was similar to pregnant group. The results revealed that mammalian form of GnRH exerted endometrial change in guinea pig almost similar to those occur in normal pregnant animals and its administration prior to implantation may improve pregnancy rate following embryo transfer.
Indian journal of experimental biology, 2007
Endometrium of GnRH treated group resembled with pregnant group and endometrial thickness in thes... more Endometrium of GnRH treated group resembled with pregnant group and endometrial thickness in these groups significantly increased in comparison with non-pregnant group. In GnRH treated animals, most of histomorphological changes in epithelial cells, glands and stroma of uterus was similar to pregnant group. The results revealed that mammalian form of GnRH exerted endometrial change in guinea pig almost similar to those occur in normal pregnant animals and its administration prior to implantation may improve pregnancy rate following embryo transfer.
Toxicology and Industrial Health
Exposure to mobile phone radiation causes deleterious health effects on biological systems. The o... more Exposure to mobile phone radiation causes deleterious health effects on biological systems. The objects of this study were to investigate the effect of 900-MHz radiofrequency waves (RFW) emitted from base transceiver station antenna on intrapancreatic homocysteine (Hcy), tumor necrosis factor-α (TNF-α), and nerve growth factor (NGF) as predisposing factors involved in pancreatic beta cell damage. Thirty male rats (Sprague-Dawley, 200 ± 10 g) were randomly divided into the control (without any exposure) and exposed groups: short time (2 h/day), long time (4 h/day), and exposed to 900-MHz RFW for 30 consecutive days. On the last days of the experiment, animals were killed and pancreas tissue was dissected out for evaluation of serotonin, Hcy, TNF-α, and NGF. There was a significant decrease in the serotonin and NGF levels in the pancreatic tissue of exposed groups compared to the control group ( p < 0.05). Also, the levels of serotonin and NGF in the long-time exposure were signifi...
Journal of Animal Physiology and Animal Nutrition
Journal of Diabetes Research
Background. Increased activity of aldose reductase (AR) is one of the mechanisms involved in the ... more Background. Increased activity of aldose reductase (AR) is one of the mechanisms involved in the development of diabetic complications. Inhibiting AR can be a target to prevent diabetes complications. This study is aimed at evaluating the effect of cyclohexane (CH) and ethanol extracts (ET) of walnut leaves on AR activity in the lens and testis of diabetic rats. Methods. Fifty-six male rats classified into seven groups as control and treatment groups and treated for 30 days. The treatment groups were treated with different concentrations of ET and CH. The diabetic control (DC) group was exposed to streptozotocin. AR activity was measured in the lens and testis. The expression of AR in the testis was evaluated by the immunohistochemistry method. Results. Both extracts significantly reduced the AR activity (ng/mg of tissue protein) in the testis (0.034±0.004, 0.038±0.010, and 0.040±0.007 in the treatment groups vs. 0.075±0.007 in the DC group) and lens (1.66±0.09, 2.70±0.47, and 1.77±...
Journal of Ovarian Research
Background Polycystic ovary syndrome (PCOS) is a complex reproduction and endocrine disorder of w... more Background Polycystic ovary syndrome (PCOS) is a complex reproduction and endocrine disorder of women in the reproductive age. Spearmint (Mentha spicata L.) has anti-androgenic activity and flaxseed (Linum usitatissimum L.) contains phytoestrogen and was reported to improve PCOS conditions. This study aimed to evaluate PCOS conditions following administration of a mixture of these two plants. Methods Twenty-four rats with regular cycles were randomly divided into four groups as control (C) and treatment-control (TC) received a combination of spearmint extract (SE) + flaxseed extract (FE). PCOS was induced in PCOS and treatment (T) groups by a single intramuscular injection of estradiol valerate. The treatment group received a combination of SE and FE for 30 days, 7 weeks after injection of estradiol valerate. Estrous cycles were monitored for 10 days and in the last day animals were sacrificed, ovaries were collected for the histomorphometric study and the serum levels of progestero...
Physiological Reports
Diabetes mellitus (DM) is a heterogeneous endocrine and metabolic disease characterized by hyperg... more Diabetes mellitus (DM) is a heterogeneous endocrine and metabolic disease characterized by hyperglycemia frequently accompanied by glycosuria, polydipsia, and polyuria and other metabolic disorder (American Diabetes Association, 2009). This disease develops as a result of the deficiencies in the insulin level or function or both and usually is related to specific changes in intracellular metabolism and morphological changes in the kidney, retina, pancreas, and other organs (American Diabetes Association, 2009; Cantley & Ashcroft, 2015). The incidence of Diabetes mellitus is rapidly increasing
Physiological Reports
Endometriosis is one of the most common reproductive diseases of women, with some important bioch... more Endometriosis is one of the most common reproductive diseases of women, with some important biochemical changes in the serum. Curcumin was reported to have anti-endometriosis, anti-inflammatory and antioxidant activity. This study explores the changes of serum levels of Cancer Antigen 125 (CA125), leptin, resistin, homocysteine, and total antioxidant capacity (TAC) in a rat model of endometriosis and the effect of curcumin treatment on these factors. Fifty female Sprague-Dawley rats (220-250 gr body weight) were randomly divided into control (received 0.3 mL of the vehicle), sham(stress of surgery + 0.3 mL of the vehicle) and three endometriosis groups as danazol treatment (7.2 mg/kg BW, IP), curcumin treatment (48 mg/kg BW, IP) and test (received 0.3 mL of the vehicle). Endometriosis was induced by surgically auto-transplantation of uterine tissue to the abdominal wall and intestinal mesentery. The animals were treated for 4 weeks. On the last day, the blood sample was collected by heart puncture, and the above-mentioned factors were measured in the sera. Leptin in the curcumin treatment group was markedly higher than all of the studied groups, except the danazol group, while there were no significant differences between other groups (P < 0.05). Level of resistin in endometriosis groups did not show significant differences with the control group (P < 0.05). There was no significant difference in the serum levels of homocysteine and CA 125 between all of the studied groups (P > 0.05). The serum levels of TAC in the control group were significantly higher than all of the studied groups (P < 0.05). Curcumin could prevent the growth of endometriosis, while there was no significant alteration on measured factors in the serum of rat with induced endometriosis. Hence follow up of these parameters in serum may not be a proper indicator to evaluate the status of endometriosis in the rat model.
Zahedan Journal of Research in Medical Sciences
Indian Journal of Pharmacology
Zahedan Journal of Research in Medical Sciences
Zahedan Journal of Research in Medical Sciences, Jul 1, 2013
Iranian Journal of Veterinary Research, 2011
ABSTRACT In pregnant mothers maternal diabetes happens when the pancreas cannot produce enough in... more ABSTRACT In pregnant mothers maternal diabetes happens when the pancreas cannot produce enough insulin, so blood glucose increases in the mother and then in the fetus, resulting in several injuries in neonates. This study was conducted to evaluate the effects of maternal diabetes on fetal cerebrum. Sixteen adult female rats were divided in two equal groups. Diabetes was induced in one group by alloxan. Both groups became pregnant by natural mating. At days 17, 18, 19 and 20 of pregnancy, the cerebrum was collected from the fetuses of all rats, also the body weight and number of fetuses was measured. Various histological parameters were determined using routine histological techniques. Results revealed a significant decrease in the ratio of gray matter to white matter and also the number of cells in gray matter and white matter in all days. There was also a significant decrease in thickness of gray matter at day 20 of pregnancy in the cerebrum of fetuses of diabetic mothers (FDM) as compared with the control group. The body weight of FDM was significantly (P<0.05) more than that of the control group and the number of fetuses in FDM was significantly (P<0.05) less than the control group. Maternal hyperglycemia exhibited deleterious effects on cerebrum during fetal life, which affected: cell number, gray matter to white matter ratio and thickness of gray and white matter. Key words: Maternal diabetes, Rat, fetus, Alloxan, Cerebrum
ABSTRACT In pregnant mothers, maternal diabetes occurs when the pancreas cannot produce enough in... more ABSTRACT In pregnant mothers, maternal diabetes occurs when the pancreas cannot produce enough insulin, which leads to increased blood glucose concentration in the mother and consequently in the foetus, causing various neonatal problems. This study was conducted to evaluate the effects of maternal diabetes on foetal ovarian structure. Sixteen adult female rats were allocated into two equal groups. Diabetes was induced in one group by alloxan agent. Both groups became pregnant by natural mating. 60 days after birth, the female offspring were terminated, the body weight and blood glucose of the animals measured and their ovaries removed. Various histological parameters were determined using histological techniques. Results revealed a significant increase in body weight and blood glucose in the offspring of the diabetic mothers (ODM) compared to that of controls. The weight, volume and diameter of the ovary and ovarian capsule thickness were decreased in the ODM group. The number and diameter of primary, preantral, antral and preovulatory follicles and corpora lutea were decreased in ovaries in the ODM. Maternal hyperglycaemia exhibited deleterious effects on the reproductive system of their offspring. Keywords: Gestational Diabetes, Rat, neonates, Ovary, Follicle
Http Dx Doi Org 10 1080 09712119 2008 9706904, Nov 14, 2011
ABSTRACT Gholami, S., Jelodar, G. and Alborzinia, H. 2008. Effect of hCG on rat endometrium at pr... more ABSTRACT Gholami, S., Jelodar, G. and Alborzinia, H. 2008. Effect of hCG on rat endometrium at preimplantation stage. J. Appl. Anim. Res., 33: 89–92.To compare histological changes in the endometrium of non pregnant, pregnant, pseudopregenant and hCG treated rat at preimplantation stage, twenty mature female rats at estrus stage were randomly divided in four groups of non pregnant (negative control), pregnant (positive control), pseudopregnant and treatment (hCG treated). Pseudopregnant and control positive groups were caged with vasectomised and intact male rat; respectively, while, control negative and test groups were not mated. Test group received hCG (10 IU) subcutaneously on second day of estrous cycle. On day 4 of estrous cycle (expected day of implantation) their uteri were subjected to histological study. There were changes in endometrial epithelium, glands and stromal cells in pseudopregnant and treatment groups, which were very similar to pregnant group. In conclusion hCG exerted moderate endometrial changes and may have potential value to improve pregnancy rate post embryo transfer.
Indian Journal of Medical Sciences, 2005
Many traditional treatments have been recommended in the alternative system of medicine for treat... more Many traditional treatments have been recommended in the alternative system of medicine for treatment of diabetes mellitus; however, the mechanism of most of the herbals used has not been defined. This study was carried out to clarify the effect of fenugreek, garlic and onion, recommended in Persian folklore medicine as beneficial in the treatment of diabetes, on blood glucose and their possible effect on pancreatic tissue. Diabetes mellitus was induced in 20 out of 25 adult male albino rats, using intraperitoneal injection of 185 mg/kg BW alloxan. The diabetic rats were divided into four groups, three of which were fed a diet containing 12.5% BW Allium sativum (garlic), Allium cepa (onion) or Trigonella foenum-graecum (fenugreek) for 15 days. The fourth group (positive control) received an ordinary diet. The remaining non-diabetic rats (negative control group) received neither alloxan nor the mentioned plants. Following consumption of plants, blood glucose was measured every day and on the last day the pancreas were removed and stained with H&E and Gomeri aldehyde fuchsin (GAF). Morphology of the pancreatic sections and the following morphometric factors were studied: volume density of B cells, volume density of islets, percent of B cells, number of islets per square millimeter, average area of islets and average volume density of B cell in whole pancreas. One-way Analysis of Variance (ANOVA) test and Duncan's multiple range tests were used to evaluate the data. The results of this study indicate that only garlic was able to reduce blood glucose significantly compared with the control group (P<0.05). In the control positive group all the mentioned morphometric factors were significantly changed in comparison with the control negative (normal health) group, but the same did not show significant change between treated and untreated diabetics.
Comparative Clinical Pathology
Application of antibiotics in livestock production has been a big concern in the recent years sin... more Application of antibiotics in livestock production has been a big concern in the recent years since they can have a vital role in the emergence of antibiotic resistant bacteria. Therefore, scientists and nutritionists have been searching for alternative agents to alter ruminal metabolism and improve feed efficiency and animal productivity. In this respect, the aim of the present study was to evaluate the effects of onion extract on leptin, ghrelin, total antioxidant capacity (TAC), and the performance of suckling lambs. Eighteen Torki Qashqai suckling lambs with 30 days of age were randomly divided into three groups, including (1) control group; basement diet without onion extract + sheep milk, (2) T150 group; basement diet containing 150 mg/kg onion extract + sheep milk, (3) T250 group; basement diet containing 250 mg/kg onion extract + sheep milk. The trial lasted for 60 days and the blood samples were taken on days 30 and 60 of the experiment. The results showed that the addition of onion extract led to an increase in the feed intake and daily weight in lambs. Moreover, onion extract was able to increase the level of ghrelin and reduce leptin hormone in the treatment groups (T150 and T250). In conclusion, it could be said that onion extract has a promising effect as a feed additive in ruminant nutrition and has the ability to improve feed intake in these animals. Moreover, onion extract has a positive effect on concentrations of leptin, ghrelin, TAC, and the performance in lambs.
Indian journal of experimental biology, 2007
Endometrium of GnRH treated group resembled with pregnant group and endometrial thickness in thes... more Endometrium of GnRH treated group resembled with pregnant group and endometrial thickness in these groups significantly increased in comparison with non-pregnant group. In GnRH treated animals, most of histomorphological changes in epithelial cells, glands and stroma of uterus was similar to pregnant group. The results revealed that mammalian form of GnRH exerted endometrial change in guinea pig almost similar to those occur in normal pregnant animals and its administration prior to implantation may improve pregnancy rate following embryo transfer.
Indian journal of experimental biology, 2007
Endometrium of GnRH treated group resembled with pregnant group and endometrial thickness in thes... more Endometrium of GnRH treated group resembled with pregnant group and endometrial thickness in these groups significantly increased in comparison with non-pregnant group. In GnRH treated animals, most of histomorphological changes in epithelial cells, glands and stroma of uterus was similar to pregnant group. The results revealed that mammalian form of GnRH exerted endometrial change in guinea pig almost similar to those occur in normal pregnant animals and its administration prior to implantation may improve pregnancy rate following embryo transfer.
Toxicology and Industrial Health
Exposure to mobile phone radiation causes deleterious health effects on biological systems. The o... more Exposure to mobile phone radiation causes deleterious health effects on biological systems. The objects of this study were to investigate the effect of 900-MHz radiofrequency waves (RFW) emitted from base transceiver station antenna on intrapancreatic homocysteine (Hcy), tumor necrosis factor-α (TNF-α), and nerve growth factor (NGF) as predisposing factors involved in pancreatic beta cell damage. Thirty male rats (Sprague-Dawley, 200 ± 10 g) were randomly divided into the control (without any exposure) and exposed groups: short time (2 h/day), long time (4 h/day), and exposed to 900-MHz RFW for 30 consecutive days. On the last days of the experiment, animals were killed and pancreas tissue was dissected out for evaluation of serotonin, Hcy, TNF-α, and NGF. There was a significant decrease in the serotonin and NGF levels in the pancreatic tissue of exposed groups compared to the control group ( p < 0.05). Also, the levels of serotonin and NGF in the long-time exposure were signifi...
Journal of Animal Physiology and Animal Nutrition
Journal of Diabetes Research
Background. Increased activity of aldose reductase (AR) is one of the mechanisms involved in the ... more Background. Increased activity of aldose reductase (AR) is one of the mechanisms involved in the development of diabetic complications. Inhibiting AR can be a target to prevent diabetes complications. This study is aimed at evaluating the effect of cyclohexane (CH) and ethanol extracts (ET) of walnut leaves on AR activity in the lens and testis of diabetic rats. Methods. Fifty-six male rats classified into seven groups as control and treatment groups and treated for 30 days. The treatment groups were treated with different concentrations of ET and CH. The diabetic control (DC) group was exposed to streptozotocin. AR activity was measured in the lens and testis. The expression of AR in the testis was evaluated by the immunohistochemistry method. Results. Both extracts significantly reduced the AR activity (ng/mg of tissue protein) in the testis (0.034±0.004, 0.038±0.010, and 0.040±0.007 in the treatment groups vs. 0.075±0.007 in the DC group) and lens (1.66±0.09, 2.70±0.47, and 1.77±...
Journal of Ovarian Research
Background Polycystic ovary syndrome (PCOS) is a complex reproduction and endocrine disorder of w... more Background Polycystic ovary syndrome (PCOS) is a complex reproduction and endocrine disorder of women in the reproductive age. Spearmint (Mentha spicata L.) has anti-androgenic activity and flaxseed (Linum usitatissimum L.) contains phytoestrogen and was reported to improve PCOS conditions. This study aimed to evaluate PCOS conditions following administration of a mixture of these two plants. Methods Twenty-four rats with regular cycles were randomly divided into four groups as control (C) and treatment-control (TC) received a combination of spearmint extract (SE) + flaxseed extract (FE). PCOS was induced in PCOS and treatment (T) groups by a single intramuscular injection of estradiol valerate. The treatment group received a combination of SE and FE for 30 days, 7 weeks after injection of estradiol valerate. Estrous cycles were monitored for 10 days and in the last day animals were sacrificed, ovaries were collected for the histomorphometric study and the serum levels of progestero...
Physiological Reports
Diabetes mellitus (DM) is a heterogeneous endocrine and metabolic disease characterized by hyperg... more Diabetes mellitus (DM) is a heterogeneous endocrine and metabolic disease characterized by hyperglycemia frequently accompanied by glycosuria, polydipsia, and polyuria and other metabolic disorder (American Diabetes Association, 2009). This disease develops as a result of the deficiencies in the insulin level or function or both and usually is related to specific changes in intracellular metabolism and morphological changes in the kidney, retina, pancreas, and other organs (American Diabetes Association, 2009; Cantley & Ashcroft, 2015). The incidence of Diabetes mellitus is rapidly increasing
Physiological Reports
Endometriosis is one of the most common reproductive diseases of women, with some important bioch... more Endometriosis is one of the most common reproductive diseases of women, with some important biochemical changes in the serum. Curcumin was reported to have anti-endometriosis, anti-inflammatory and antioxidant activity. This study explores the changes of serum levels of Cancer Antigen 125 (CA125), leptin, resistin, homocysteine, and total antioxidant capacity (TAC) in a rat model of endometriosis and the effect of curcumin treatment on these factors. Fifty female Sprague-Dawley rats (220-250 gr body weight) were randomly divided into control (received 0.3 mL of the vehicle), sham(stress of surgery + 0.3 mL of the vehicle) and three endometriosis groups as danazol treatment (7.2 mg/kg BW, IP), curcumin treatment (48 mg/kg BW, IP) and test (received 0.3 mL of the vehicle). Endometriosis was induced by surgically auto-transplantation of uterine tissue to the abdominal wall and intestinal mesentery. The animals were treated for 4 weeks. On the last day, the blood sample was collected by heart puncture, and the above-mentioned factors were measured in the sera. Leptin in the curcumin treatment group was markedly higher than all of the studied groups, except the danazol group, while there were no significant differences between other groups (P < 0.05). Level of resistin in endometriosis groups did not show significant differences with the control group (P < 0.05). There was no significant difference in the serum levels of homocysteine and CA 125 between all of the studied groups (P > 0.05). The serum levels of TAC in the control group were significantly higher than all of the studied groups (P < 0.05). Curcumin could prevent the growth of endometriosis, while there was no significant alteration on measured factors in the serum of rat with induced endometriosis. Hence follow up of these parameters in serum may not be a proper indicator to evaluate the status of endometriosis in the rat model.
Zahedan Journal of Research in Medical Sciences
Indian Journal of Pharmacology
Zahedan Journal of Research in Medical Sciences
Zahedan Journal of Research in Medical Sciences, Jul 1, 2013
Iranian Journal of Veterinary Research, 2011
ABSTRACT In pregnant mothers maternal diabetes happens when the pancreas cannot produce enough in... more ABSTRACT In pregnant mothers maternal diabetes happens when the pancreas cannot produce enough insulin, so blood glucose increases in the mother and then in the fetus, resulting in several injuries in neonates. This study was conducted to evaluate the effects of maternal diabetes on fetal cerebrum. Sixteen adult female rats were divided in two equal groups. Diabetes was induced in one group by alloxan. Both groups became pregnant by natural mating. At days 17, 18, 19 and 20 of pregnancy, the cerebrum was collected from the fetuses of all rats, also the body weight and number of fetuses was measured. Various histological parameters were determined using routine histological techniques. Results revealed a significant decrease in the ratio of gray matter to white matter and also the number of cells in gray matter and white matter in all days. There was also a significant decrease in thickness of gray matter at day 20 of pregnancy in the cerebrum of fetuses of diabetic mothers (FDM) as compared with the control group. The body weight of FDM was significantly (P<0.05) more than that of the control group and the number of fetuses in FDM was significantly (P<0.05) less than the control group. Maternal hyperglycemia exhibited deleterious effects on cerebrum during fetal life, which affected: cell number, gray matter to white matter ratio and thickness of gray and white matter. Key words: Maternal diabetes, Rat, fetus, Alloxan, Cerebrum
ABSTRACT In pregnant mothers, maternal diabetes occurs when the pancreas cannot produce enough in... more ABSTRACT In pregnant mothers, maternal diabetes occurs when the pancreas cannot produce enough insulin, which leads to increased blood glucose concentration in the mother and consequently in the foetus, causing various neonatal problems. This study was conducted to evaluate the effects of maternal diabetes on foetal ovarian structure. Sixteen adult female rats were allocated into two equal groups. Diabetes was induced in one group by alloxan agent. Both groups became pregnant by natural mating. 60 days after birth, the female offspring were terminated, the body weight and blood glucose of the animals measured and their ovaries removed. Various histological parameters were determined using histological techniques. Results revealed a significant increase in body weight and blood glucose in the offspring of the diabetic mothers (ODM) compared to that of controls. The weight, volume and diameter of the ovary and ovarian capsule thickness were decreased in the ODM group. The number and diameter of primary, preantral, antral and preovulatory follicles and corpora lutea were decreased in ovaries in the ODM. Maternal hyperglycaemia exhibited deleterious effects on the reproductive system of their offspring. Keywords: Gestational Diabetes, Rat, neonates, Ovary, Follicle
Http Dx Doi Org 10 1080 09712119 2008 9706904, Nov 14, 2011
ABSTRACT Gholami, S., Jelodar, G. and Alborzinia, H. 2008. Effect of hCG on rat endometrium at pr... more ABSTRACT Gholami, S., Jelodar, G. and Alborzinia, H. 2008. Effect of hCG on rat endometrium at preimplantation stage. J. Appl. Anim. Res., 33: 89–92.To compare histological changes in the endometrium of non pregnant, pregnant, pseudopregenant and hCG treated rat at preimplantation stage, twenty mature female rats at estrus stage were randomly divided in four groups of non pregnant (negative control), pregnant (positive control), pseudopregnant and treatment (hCG treated). Pseudopregnant and control positive groups were caged with vasectomised and intact male rat; respectively, while, control negative and test groups were not mated. Test group received hCG (10 IU) subcutaneously on second day of estrous cycle. On day 4 of estrous cycle (expected day of implantation) their uteri were subjected to histological study. There were changes in endometrial epithelium, glands and stromal cells in pseudopregnant and treatment groups, which were very similar to pregnant group. In conclusion hCG exerted moderate endometrial changes and may have potential value to improve pregnancy rate post embryo transfer.