Hirokazu Kawagishi | Shizuoka University (original) (raw)

Papers by Hirokazu Kawagishi

Research paper thumbnail of Erinacines E, F, and G, Stimulators of Nerve Growth Factor (NGF)-Synthesis, from the Mycelia of Hericium erinaceum

岐阜藥科大學紀要, Jun 30, 1997

The structures of erinacines E, F and G from mycelia of Hericium erinaceum were determined by spe... more The structures of erinacines E, F and G from mycelia of Hericium erinaceum were determined by spectroscopic and/or X-ray analysis. Erinacines E and F exhibited potent stimulating activity against NGF synthesis by astroglial cells.

Research paper thumbnail of Inhibition of IgE-dependent Mouse Triphasic Cutaneous Reaction by a Boiling Water Fraction Separated from Mycelium of Phellinus linteus

Phellinus linteus, a mushroom, contains constituents that exhibit potent antitumor effects throug... more Phellinus linteus, a mushroom, contains constituents that exhibit potent antitumor effects through activating immune cells. Recently, anti-inflammatory and anti-allergic properties of P. linteus extracts have also been implicated. In the present study, therefore, we separated the constituents of mycelium of P. linteus into five fractions—chloroform-soluble (CF), ethyl acetate-soluble (EA), methanol- soluble (AE), water-soluble (WA) and boiling water-soluble (BW) fractions—and examined

Research paper thumbnail of Bioconversion of AHX to AOH by resting cells of Burkholderia contaminans CH-1

Bioscience, biotechnology, and biochemistry, Jan 7, 2016

Fairy rings are zones of stimulated grass growth owing to the interaction between a fungus and a ... more Fairy rings are zones of stimulated grass growth owing to the interaction between a fungus and a plant. We previously reported the discovery of two novel plant-growth regulating compounds related to forming fairy rings, 2-azahypoxanthine (AHX) and 2-aza-8-oxohypoxanthine (AOH). In this study, a bacterial strain CH-1 was isolated from an airborne-contaminated nutrient medium containing AHX. The strain converted AHX to AOH and identified as Burkholderia contaminans based on the gene sequence of its 16S rDNA. The quantitative production of AOH by resting cells of the strain was achieved. Among seven Burkholderia species, two bacteria and two yeasts tested, B. contaminans CH-1 showed the highest rate of conversion of AHX to AOH. By batch system, up to 10.6 mmol AHX was converted to AOH using the resting cells. The yield of this process reached at 91%.

Research paper thumbnail of Lactosylamidine-based affinity purification for cellulolytic enzymes EG I and CBH I from Hypocrea jecorina and their properties

Carbohydrate Research, Nov 1, 2010

Selective adsorption and separation of β-glucosidase, endo-acting endo-β-(1→4)-glucanase I (EG I)... more Selective adsorption and separation of β-glucosidase, endo-acting endo-β-(1→4)-glucanase I (EG I), and exo-acting cellobiohydrolase I (CBH I) were achieved by affinity chromatography with β-lactosylamidine as ligand. A crude cellulase preparation from Hypocrea jecorina served as the source of enzyme. When crude cellulase was applied to the lactosylamidine-based affinity column, β-glucosidase appeared in the unbound fraction. By contrast, EG I and CBH I were retained on the column and then separated from each other by appropriately adjusting the elution conditions. The relative affinities of the enzymes, based on their column elution conditions, were strongly dependent on the ligand. The highly purified EG I and CBH I, obtained by affinity chromatography, were further purified by Mono P and DEAE chromatography, respectively. EG I and CBH I cleave only at the phenolic bond in p-nitrophenyl glycosides with lactose and N-acetyllactosamine (LacNAc). By contrast, both scissile bonds in p-nitrophenyl glycosides with cellobiose were subject to hydrolysis although with important differences in their kinetic parameters.

Research paper thumbnail of Chaxines B, C, D, and E from the edible mushroom Agrocybe chaxingu

Tetrahedron, Nov 21, 2009

1

Research paper thumbnail of The Anti-Dementia effect of Lion's Mane mushroom (Hericium erinaceum) and its clinical application.(Lion's Mane)

Townsend Letter For Doctors and Patients, Apr 1, 2004

Research paper thumbnail of Fucose 1 6-specific lectin

Research paper thumbnail of Preparation of Chitooligosaccharide-alditols by Hydrogenation with a Ruthenium Catalyst

Bioscience Biotechnology and Biochemistry, Jun 12, 2014

Research paper thumbnail of Production stimulators of nerve growth factors comprising cyathane derivatives

Research paper thumbnail of Blastocyst formation by pig embryos resulting from in-vitro fertilization of oocytes maturated in vitro

Journal of Reproduction and Fertility, 1990

Follicular oocytes collected from prepubertal gilts at a local slaughter house were matured (36 h... more Follicular oocytes collected from prepubertal gilts at a local slaughter house were matured (36 h), fertilized and developed in vitro. Of 785 embryos, 190 (24%) embryos cleaved to the 2\p=n-\4-cell stages with blastomeres of regular size by 33 h after insemination. These cleaved embryos were surgically transferred into the oviducts of 4 synchronized recipient gilts and recovered from the uterine horns 4 or 7 days later: 13 morulae, 2 blastocysts and 1 expanded blastocyst were recovered after 4 days and 3 hatched blastocysts were recovered 7 days after transfer. Re-culture in vitro sustained further development of morulae recovered 4 days after transfer: 11 of 13 morulae had developed to the blastocyst/hatched blastocyst stages. Overall, 17 of 190 (9%) embryos developed to the blastocyst stage. The results indicate that pig oocytes can be matured and fertilized in vitro, and subsequently develop to the blastocyst stage.

Research paper thumbnail of Enzymatic synthesis of p-nitrophenyl 35-O-β-N-acetylglucosaminyl|-α-maltopentaoside by lysozyme; a novel substrate for human amylase assay

Biochimica Et Biophysica Acta, 1990

Research paper thumbnail of Anti-aging and anti-microbial effects of melleolide on various types of yeast

Http Dx Doi Org 10 1080 09168451 2014 885826, Mar 1, 2014

The chronological lifespan (CLS) of the budding yeast Saccharomyces cerevisiae is a model for the... more The chronological lifespan (CLS) of the budding yeast Saccharomyces cerevisiae is a model for the aging of post-mitotic cells in higher eukaryotes. In this study, we found that the sesquiterpene aryl ester melleolide expands the CLS of budding yeast. In contrast, melleolide compromised the CLS of the fission yeast Schizosaccharomyces pombe. This indicates that melleolide might have a potential anti-aging activity against some types of cell, and that it might be useful as a selective anti-fungal drug.

Research paper thumbnail of L-Fucose ALPHA1-6 Specific Lectin

Research paper thumbnail of Preparation of N-Acetylchitooligosaccharides from Enzymatic Hydrolyzates of Chitosan

Biosci Biotechnol Biochem, 1992

Research paper thumbnail of Improvement of ethanol production by recombinant expression of pyruvate decarboxylase in the white-rot fungus Phanerochaete sordida YK-624

Journal of bioscience and bioengineering, 2016

To improve ethanol production by Phanerochaete sordida YK-624, the pyruvate decarboxylase (PDC) g... more To improve ethanol production by Phanerochaete sordida YK-624, the pyruvate decarboxylase (PDC) gene was cloned from and reintroduced into this hyper lignin-degrading fungus; the gene encodes a key enzyme in alcoholic fermentation. We screened 16 transformant P. sordida YK-624 strains that each expressed a second, recombinant PDC gene (pdc) and then identified the transformant strain (designated GP7) with the highest ethanol production. Direct ethanol production from hardwood was 1.41 higher with GP7 than with wild-type P. sordida YK-624. RT-PCR analysis indicated that the increased PDC activity was caused by elevated recombinant pdc expression. Taken together, these results suggested that ethanol production by P. sordida YK-624 can be improved by the stable expression of an additional, recombinant pdc.

Research paper thumbnail of Effects of Homologous Expression of 1,4-Benzoquinone Reductase and Homogentisate 1,2-Dioxygenase Genes on Wood Decay in Hyper-Lignin-Degrading Fungus Phanerochaete sordida YK-624

Current microbiology, Jan 30, 2016

We investigated the function of 1,4-benzoquinone reductase (BQR)- and homogentisate 1,2-dioxygena... more We investigated the function of 1,4-benzoquinone reductase (BQR)- and homogentisate 1,2-dioxygenase (HGD)-like genes in wood degradation by Phanerochaete sordida YK-624, which exhibits high ligninolytic activity and selectivity. We determined homologous expression in the genomic and cDNA sequences of BQR- and HGD-like genes in P. sordida YK-624 (PsBQR and PsHGD). Both genes shared high homology (≥90 % amino acid sequence similarity) with the corresponding genes in Phanerochaete chrysosporium. These genes were co-transformed with a reporter gene into an uracil auxotrophic mutant of P. sordida YK-624. The PsBQR and PsHGD co-transformants exhibited lower holocellulolytic activity and higher ligninolytic selectivity than the control transformants. In liquid culture with vanillin, both co-transformants significantly accelerated vanillin degradation. Thus, we suggest that the rapid metabolism of low-molecular weight lignin fragments, due to the homologous expression of BQR- and HGD-like g...

Research paper thumbnail of N-Acetylglucosaminyl Disaccharide and Trisaccharide Formation Through Lysozyme-Catalyzed Transfer Reaction

Http Dx Doi Org 10 1080 07328309508002064, Aug 21, 2006

Research paper thumbnail of A pyradine-derivative from the mushroom Albatrellus confluens

Phytochemistry, May 31, 1996

Pergamon 00319422(95)008810 Phytochemisttj', Vol. 42, No. 2, pp. 547548, 1996 Copyright 1996... more Pergamon 00319422(95)008810 Phytochemisttj', Vol. 42, No. 2, pp. 547548, 1996 Copyright 1996 Elsevier Science Ltd Printed in Great Britain. All rights reserved 00319422 96 15.00 + 0.00 A PYRADINEDERIVATIVE FROM THE MUSHROOM ALBATRELLUS CONFLUENS ...

Research paper thumbnail of Hypocholesterolemic Action of Dietary Grifolin on Rats Fed with a High-cholesterol Diet

Bioscience Biotechnology and Biochemistry, Jun 12, 2014

Research paper thumbnail of Effect of Capsianoside, a Diterpene Glycoside, on Tight-Junctional Permeability

Biochimica Et Biophysica Acta Biomembranes, Jan 31, 1997

. Previous work Hashimoto et al., 1994 Biosci. Biotech. Biochem. 58, 1345 revealed that a sweet p... more . Previous work Hashimoto et al., 1994 Biosci. Biotech. Biochem. 58, 1345 revealed that a sweet pepper extract Ž . enhanced the tight-junctional TJ permeability of a human intestinal Caco-2 cell monolayer. In the present study, the substance which modulated the TJ permeability was chromatographically purified from the extract. The active substances were identified as capsianosides A-F, diterpene glycosides. Treatment of the cells with capsianoside F, the most active compound, decreased the cellular G-actin content by 40% and increased the F-actin content by 16%. The effect of Ž capsianoside F was significantly suppressed by disturbing the cytoskeletal structure with cytochalasin D at a low dose 50 . ngrml . These results suggest that capsianosides affected the cytoskeletal function by modulating the reorganization of actin filaments, by which the TJ structure and permeability were changed. The possible involvement of a PKC inhibition in the mechanism of an increase in TJ permeability is also suggested.

Research paper thumbnail of Erinacines E, F, and G, Stimulators of Nerve Growth Factor (NGF)-Synthesis, from the Mycelia of Hericium erinaceum

岐阜藥科大學紀要, Jun 30, 1997

The structures of erinacines E, F and G from mycelia of Hericium erinaceum were determined by spe... more The structures of erinacines E, F and G from mycelia of Hericium erinaceum were determined by spectroscopic and/or X-ray analysis. Erinacines E and F exhibited potent stimulating activity against NGF synthesis by astroglial cells.

Research paper thumbnail of Inhibition of IgE-dependent Mouse Triphasic Cutaneous Reaction by a Boiling Water Fraction Separated from Mycelium of Phellinus linteus

Phellinus linteus, a mushroom, contains constituents that exhibit potent antitumor effects throug... more Phellinus linteus, a mushroom, contains constituents that exhibit potent antitumor effects through activating immune cells. Recently, anti-inflammatory and anti-allergic properties of P. linteus extracts have also been implicated. In the present study, therefore, we separated the constituents of mycelium of P. linteus into five fractions—chloroform-soluble (CF), ethyl acetate-soluble (EA), methanol- soluble (AE), water-soluble (WA) and boiling water-soluble (BW) fractions—and examined

Research paper thumbnail of Bioconversion of AHX to AOH by resting cells of Burkholderia contaminans CH-1

Bioscience, biotechnology, and biochemistry, Jan 7, 2016

Fairy rings are zones of stimulated grass growth owing to the interaction between a fungus and a ... more Fairy rings are zones of stimulated grass growth owing to the interaction between a fungus and a plant. We previously reported the discovery of two novel plant-growth regulating compounds related to forming fairy rings, 2-azahypoxanthine (AHX) and 2-aza-8-oxohypoxanthine (AOH). In this study, a bacterial strain CH-1 was isolated from an airborne-contaminated nutrient medium containing AHX. The strain converted AHX to AOH and identified as Burkholderia contaminans based on the gene sequence of its 16S rDNA. The quantitative production of AOH by resting cells of the strain was achieved. Among seven Burkholderia species, two bacteria and two yeasts tested, B. contaminans CH-1 showed the highest rate of conversion of AHX to AOH. By batch system, up to 10.6 mmol AHX was converted to AOH using the resting cells. The yield of this process reached at 91%.

Research paper thumbnail of Lactosylamidine-based affinity purification for cellulolytic enzymes EG I and CBH I from Hypocrea jecorina and their properties

Carbohydrate Research, Nov 1, 2010

Selective adsorption and separation of β-glucosidase, endo-acting endo-β-(1→4)-glucanase I (EG I)... more Selective adsorption and separation of β-glucosidase, endo-acting endo-β-(1→4)-glucanase I (EG I), and exo-acting cellobiohydrolase I (CBH I) were achieved by affinity chromatography with β-lactosylamidine as ligand. A crude cellulase preparation from Hypocrea jecorina served as the source of enzyme. When crude cellulase was applied to the lactosylamidine-based affinity column, β-glucosidase appeared in the unbound fraction. By contrast, EG I and CBH I were retained on the column and then separated from each other by appropriately adjusting the elution conditions. The relative affinities of the enzymes, based on their column elution conditions, were strongly dependent on the ligand. The highly purified EG I and CBH I, obtained by affinity chromatography, were further purified by Mono P and DEAE chromatography, respectively. EG I and CBH I cleave only at the phenolic bond in p-nitrophenyl glycosides with lactose and N-acetyllactosamine (LacNAc). By contrast, both scissile bonds in p-nitrophenyl glycosides with cellobiose were subject to hydrolysis although with important differences in their kinetic parameters.

Research paper thumbnail of Chaxines B, C, D, and E from the edible mushroom Agrocybe chaxingu

Tetrahedron, Nov 21, 2009

1

Research paper thumbnail of The Anti-Dementia effect of Lion's Mane mushroom (Hericium erinaceum) and its clinical application.(Lion's Mane)

Townsend Letter For Doctors and Patients, Apr 1, 2004

Research paper thumbnail of Fucose 1 6-specific lectin

Research paper thumbnail of Preparation of Chitooligosaccharide-alditols by Hydrogenation with a Ruthenium Catalyst

Bioscience Biotechnology and Biochemistry, Jun 12, 2014

Research paper thumbnail of Production stimulators of nerve growth factors comprising cyathane derivatives

Research paper thumbnail of Blastocyst formation by pig embryos resulting from in-vitro fertilization of oocytes maturated in vitro

Journal of Reproduction and Fertility, 1990

Follicular oocytes collected from prepubertal gilts at a local slaughter house were matured (36 h... more Follicular oocytes collected from prepubertal gilts at a local slaughter house were matured (36 h), fertilized and developed in vitro. Of 785 embryos, 190 (24%) embryos cleaved to the 2\p=n-\4-cell stages with blastomeres of regular size by 33 h after insemination. These cleaved embryos were surgically transferred into the oviducts of 4 synchronized recipient gilts and recovered from the uterine horns 4 or 7 days later: 13 morulae, 2 blastocysts and 1 expanded blastocyst were recovered after 4 days and 3 hatched blastocysts were recovered 7 days after transfer. Re-culture in vitro sustained further development of morulae recovered 4 days after transfer: 11 of 13 morulae had developed to the blastocyst/hatched blastocyst stages. Overall, 17 of 190 (9%) embryos developed to the blastocyst stage. The results indicate that pig oocytes can be matured and fertilized in vitro, and subsequently develop to the blastocyst stage.

Research paper thumbnail of Enzymatic synthesis of p-nitrophenyl 35-O-β-N-acetylglucosaminyl|-α-maltopentaoside by lysozyme; a novel substrate for human amylase assay

Biochimica Et Biophysica Acta, 1990

Research paper thumbnail of Anti-aging and anti-microbial effects of melleolide on various types of yeast

Http Dx Doi Org 10 1080 09168451 2014 885826, Mar 1, 2014

The chronological lifespan (CLS) of the budding yeast Saccharomyces cerevisiae is a model for the... more The chronological lifespan (CLS) of the budding yeast Saccharomyces cerevisiae is a model for the aging of post-mitotic cells in higher eukaryotes. In this study, we found that the sesquiterpene aryl ester melleolide expands the CLS of budding yeast. In contrast, melleolide compromised the CLS of the fission yeast Schizosaccharomyces pombe. This indicates that melleolide might have a potential anti-aging activity against some types of cell, and that it might be useful as a selective anti-fungal drug.

Research paper thumbnail of L-Fucose ALPHA1-6 Specific Lectin

Research paper thumbnail of Preparation of N-Acetylchitooligosaccharides from Enzymatic Hydrolyzates of Chitosan

Biosci Biotechnol Biochem, 1992

Research paper thumbnail of Improvement of ethanol production by recombinant expression of pyruvate decarboxylase in the white-rot fungus Phanerochaete sordida YK-624

Journal of bioscience and bioengineering, 2016

To improve ethanol production by Phanerochaete sordida YK-624, the pyruvate decarboxylase (PDC) g... more To improve ethanol production by Phanerochaete sordida YK-624, the pyruvate decarboxylase (PDC) gene was cloned from and reintroduced into this hyper lignin-degrading fungus; the gene encodes a key enzyme in alcoholic fermentation. We screened 16 transformant P. sordida YK-624 strains that each expressed a second, recombinant PDC gene (pdc) and then identified the transformant strain (designated GP7) with the highest ethanol production. Direct ethanol production from hardwood was 1.41 higher with GP7 than with wild-type P. sordida YK-624. RT-PCR analysis indicated that the increased PDC activity was caused by elevated recombinant pdc expression. Taken together, these results suggested that ethanol production by P. sordida YK-624 can be improved by the stable expression of an additional, recombinant pdc.

Research paper thumbnail of Effects of Homologous Expression of 1,4-Benzoquinone Reductase and Homogentisate 1,2-Dioxygenase Genes on Wood Decay in Hyper-Lignin-Degrading Fungus Phanerochaete sordida YK-624

Current microbiology, Jan 30, 2016

We investigated the function of 1,4-benzoquinone reductase (BQR)- and homogentisate 1,2-dioxygena... more We investigated the function of 1,4-benzoquinone reductase (BQR)- and homogentisate 1,2-dioxygenase (HGD)-like genes in wood degradation by Phanerochaete sordida YK-624, which exhibits high ligninolytic activity and selectivity. We determined homologous expression in the genomic and cDNA sequences of BQR- and HGD-like genes in P. sordida YK-624 (PsBQR and PsHGD). Both genes shared high homology (≥90 % amino acid sequence similarity) with the corresponding genes in Phanerochaete chrysosporium. These genes were co-transformed with a reporter gene into an uracil auxotrophic mutant of P. sordida YK-624. The PsBQR and PsHGD co-transformants exhibited lower holocellulolytic activity and higher ligninolytic selectivity than the control transformants. In liquid culture with vanillin, both co-transformants significantly accelerated vanillin degradation. Thus, we suggest that the rapid metabolism of low-molecular weight lignin fragments, due to the homologous expression of BQR- and HGD-like g...

Research paper thumbnail of N-Acetylglucosaminyl Disaccharide and Trisaccharide Formation Through Lysozyme-Catalyzed Transfer Reaction

Http Dx Doi Org 10 1080 07328309508002064, Aug 21, 2006

Research paper thumbnail of A pyradine-derivative from the mushroom Albatrellus confluens

Phytochemistry, May 31, 1996

Pergamon 00319422(95)008810 Phytochemisttj', Vol. 42, No. 2, pp. 547548, 1996 Copyright 1996... more Pergamon 00319422(95)008810 Phytochemisttj', Vol. 42, No. 2, pp. 547548, 1996 Copyright 1996 Elsevier Science Ltd Printed in Great Britain. All rights reserved 00319422 96 15.00 + 0.00 A PYRADINEDERIVATIVE FROM THE MUSHROOM ALBATRELLUS CONFLUENS ...

Research paper thumbnail of Hypocholesterolemic Action of Dietary Grifolin on Rats Fed with a High-cholesterol Diet

Bioscience Biotechnology and Biochemistry, Jun 12, 2014

Research paper thumbnail of Effect of Capsianoside, a Diterpene Glycoside, on Tight-Junctional Permeability

Biochimica Et Biophysica Acta Biomembranes, Jan 31, 1997

. Previous work Hashimoto et al., 1994 Biosci. Biotech. Biochem. 58, 1345 revealed that a sweet p... more . Previous work Hashimoto et al., 1994 Biosci. Biotech. Biochem. 58, 1345 revealed that a sweet pepper extract Ž . enhanced the tight-junctional TJ permeability of a human intestinal Caco-2 cell monolayer. In the present study, the substance which modulated the TJ permeability was chromatographically purified from the extract. The active substances were identified as capsianosides A-F, diterpene glycosides. Treatment of the cells with capsianoside F, the most active compound, decreased the cellular G-actin content by 40% and increased the F-actin content by 16%. The effect of Ž capsianoside F was significantly suppressed by disturbing the cytoskeletal structure with cytochalasin D at a low dose 50 . ngrml . These results suggest that capsianosides affected the cytoskeletal function by modulating the reorganization of actin filaments, by which the TJ structure and permeability were changed. The possible involvement of a PKC inhibition in the mechanism of an increase in TJ permeability is also suggested.