Dr. Shubhangee S . Gaikwad | Sinhgad Technical Education Society,Pune University (original) (raw)
Papers by Dr. Shubhangee S . Gaikwad
International Journal of Mass Spectrometry, Dec 31, 2023
Study reports that the, stability indicating high-performance thin layer chromatographic techniqu... more Study reports that the, stability indicating high-performance thin layer chromatographic technique (HPTLC) was developed as well as validated for estimating Paracetamol and Prochlorperazine in bulk and dosage form. These drugs are largely used in migraine and first line treatment of schizophrenia. Selected drug combination employed for Quality by design based Box Behnken design by high performance thin layer chromatography method utilizing aluminium plates; pre-coated using silica gel 60F 254 as stationary phase by using acetone: toluene: methanol (4:4:2 v/v/v) as a mobile phase. Densitogram shows R f values for Paracetamol at 0.74 and for Prochlorperazine at 0.32 evaluated by densitometry measurement bands at 248 nm. Paracetamol and Prochlorperazine marketed formulation of 700 mg by using various excipients prepared at lab scale and it was evaluated for various types of tablet tests, and it showed the accurate result with respect to standard guidelines. The approach has been validated in accordance with International Conference on Harmonization (ICH) requirements. The correlation coefficient was found to be 0.992 and 0.998 at a concentration range of 100-300 μg/ band and 1000-3000 ng/band for Paracetamol and Prochlorperazine, respectively. The method had an accuracy of 100.51 % and 100.53 % for Paracetamol and Prochlorperazine, respectively. The degradation of Paracetamol and Prochlorperazine was carried out in an acid, alkaline, oxidative, thermal and photolytic environment. The stress degraded product was successfully separated using the HPTLC and using high resonance mass spectroscopy technique (HRMS-MS), the degradation product was discovered.
BioNanoScience
Diabetes mellitus is a metabolic disorder in which a person fails to produce adequate insulin or ... more Diabetes mellitus is a metabolic disorder in which a person fails to produce adequate insulin or insulin reactions to cells, so that blood sugar levels are high in diabetes patients. Nanoparticles of small particle size have very significant effects against other types of dosage. Diabetes is treated with metal oxide-like nanoparticles such as zinc oxide, silver oxide, cerium oxide, magnesium oxide, vanadium oxide, chromium oxide, and gold nanoparticles. Different plants are used for the green synthesis of zinc, silver, magnesium oxide, cerium oxide, and golden nanoparticles. These analyses include separate in vitro and in vivo tests. The in vitro studies include nanoparticles characterization, antioxidant studies, anti-diabetic studies, and green synthesized nanoparticles phytochemical studies. In the in vivo trials, the animal research involves a range of test models, diabetes induction, experimental design, sample collection, and blood sample characterization by different testing methods. Studies in in vitro and in vivo reveal nanoparticles of metal oxide to be anti-diabetic.
Social Science Research Network, 2022
Highlights Metal Oxide (MO) nanoparticles were synthesized using a green synthetic method and c... more Highlights Metal Oxide (MO) nanoparticles were synthesized using a green synthetic method and characterized. The catalytic property of the synthesized MO nanoparticles were examined. A green protocol for the reduction of aromatic aldehydes is demonstrated.
Indian drugs, Dec 27, 2022
Reversed phase high performance liquid chromatography (RP-HPLC) method for the quantitative deter... more Reversed phase high performance liquid chromatography (RP-HPLC) method for the quantitative determination of saxagliptin (SXG) in human urine was developed and validated to support clinical studies. Chromatographic separation was achieved on an Inertsil® column (250 mm x 4.6 mm, 5 µm). Isocratic elution using a mobile phase of potassium dihydrogen phosphate buffer pH (3) - acetonitrile (80:20, V/V) at a flow rate of 1 mL min-1 with UV detection at 212.1 nm was performed. The retention time of saxagliptin was 6.4 min. The method was validated according to United State Food and Drug Administration (USFDA) (May-2001) guidelines. The developed bioanalytical method was found to be selective, accurate, precise, and having good extraction efficiency. The developed method was satisfactorily applied to the routine quality control analysis of the saxagliptin.
Study reports that the, stability indicating high-performance thin layer chromatographic techniqu... more Study reports that the, stability indicating high-performance thin layer chromatographic technique (HPTLC) was developed as well as validated for estimating Paracetamol and Prochlorperazine in bulk and dosage form. These drugs are largely used in migraine and first line treatment of schizophrenia. Selected drug combination employed for Quality by design based Box Behnken design by high performance thin layer chromatography method utilizing aluminium plates; pre-coated using silica gel 60F 254 as stationary phase by using acetone: toluene: methanol (4:4:2 v/v/v) as a mobile phase. Densitogram shows R f values for Paracetamol at 0.74 and for Prochlorperazine at 0.32 evaluated by densitometry measurement bands at 248 nm. Paracetamol and Prochlorperazine marketed formulation of 700 mg by using various excipients prepared at lab scale and it was evaluated for various types of tablet tests, and it showed the accurate result with respect to standard guidelines. The approach has been validated in accordance with International Conference on Harmonization (ICH) requirements. The correlation coefficient was found to be 0.992 and 0.998 at a concentration range of 100-300 μg/ band and 1000-3000 ng/band for Paracetamol and Prochlorperazine, respectively. The method had an accuracy of 100.51 % and 100.53 % for Paracetamol and Prochlorperazine, respectively. The degradation of Paracetamol and Prochlorperazine was carried out in an acid, alkaline, oxidative, thermal and photolytic environment. The stress degraded product was successfully separated using the HPTLC and using high resonance mass spectroscopy technique (HRMS-MS), the degradation product was discovered.
Diabetes mellitus is a metabolic disorder in which a person fails to produce adequate insulin or ... more Diabetes mellitus is a metabolic disorder in which a person fails to produce adequate insulin or insulin reactions to cells, so that blood sugar levels are high in diabetes patients. Nanoparticles of small particle size have very significant effects against other types of dosage. Diabetes is treated with metal oxide-like nanoparticles such as zinc oxide, silver oxide, cerium oxide, magnesium oxide, vanadium oxide, chromium oxide, and gold nanoparticles. Different plants are used for the green synthesis of zinc, silver, magnesium oxide, cerium oxide, and golden nanoparticles. These analyses include separate in vitro and in vivo tests. The in vitro studies include nanoparticles characterization, antioxidant studies, anti-diabetic studies, and green synthesized nanoparticles phytochemical studies. In the in vivo trials, the animal research involves a range of test models, diabetes induction, experimental design, sample collection, and blood sample characterization by different testing methods. Studies in in vitro and in vivo reveal nanoparticles of metal oxide to be anti-diabetic.
Indian Drugs
Reversed phase high performance liquid chromatography (RP-HPLC) method for the quantitative deter... more Reversed phase high performance liquid chromatography (RP-HPLC) method for the quantitative determination of saxagliptin (SXG) in human urine was developed and validated to support clinical studies. Chromatographic separation was achieved on an Inertsil® column (250 mm x 4.6 mm, 5 µm). Isocratic elution using a mobile phase of potassium dihydrogen phosphate buffer pH (3) - acetonitrile (80:20, V/V) at a flow rate of 1 mL min-1 with UV detection at 212.1 nm was performed. The retention time of saxagliptin was 6.4 min. The method was validated according to United State Food and Drug Administration (USFDA) (May-2001) guidelines. The developed bioanalytical method was found to be selective, accurate, precise, and having good extraction efficiency. The developed method was satisfactorily applied to the routine quality control analysis of the saxagliptin.
J Incl Phenom Macrocycl Chem, 2008
International Journal Of Pharmaceutical Research And Allied Sciences
Inorganic Chemistry Communications, 2022
International journal of applied research, 2018
Diabetic peripheral neuropathy is a long-term complication of diabetes. Reflexology for neuropath... more Diabetic peripheral neuropathy is a long-term complication of diabetes. Reflexology for neuropathatic pain works with the help of chemical, electrical, and nervous systems of the body – sending messages across to balance organs and parts in far reaching areas of the body. With neuropathy, reflexology has a great option of gently activating the damaged nerve fibers to send and receive correct nerve signals. Purpose: To assess the effect of foot reflexology on.neuropathic pain among diabetic patients. Objectives: To assess peripheral neuropathic pain before and after foot reflexology among diabetic patients, to compare peripheral neuropathic pain before and after foot reflexology among diabetic patients. Method: This was quantitative Quasi- experimental pre test post test control group design of 30 patients who were diabetic and had peripheral neuropathic pain, aged 31-60 years. The patients selected for study were diabetic patients who were suffering from peripheral neuropathic pain ...
INDIAN DRUGS
A simple stability indicating RP-HPLC method was validated for determination of ipratropium bromi... more A simple stability indicating RP-HPLC method was validated for determination of ipratropium bromide in the bulk drug. The drug was resolved using HPLC Column (Kromasil ODS 150 x 4.6 mm C18 column) with mobile phase of HPLC grade acetonitrile:potassium di-hydrogen phosphate buffer (60:40 V/V) at a flow rate of 1 ml/min. The retention time of ipratropium bromide was 3.7 min with UV detection at 254 nm. The method was validated with respect to linearity, sensitivity, accuracy, precision and robustness as per the International Conference on Harmonization (ICH) guidelines. The method was specific and it was observed that no interference with diluents. The linearity was established over the concentration range of 20-120 μg/ml with correlation coefficients (r2) 0.9958 for ipratropium bromide. The mean recovery was found to be in the range of 99.8% for ipratropium bromide. The % R.S.D. values for intraday precision study and inter-day study were <1.0%, confirming that the method was suff...
JPC – Journal of Planar Chromatography – Modern TLC
Journal of Current Pharma Research
A simple, sensitive and accurate spectrophotometric method has been developed for the determinati... more A simple, sensitive and accurate spectrophotometric method has been developed for the determination of Acebrophylline in bulk drug and capsule. The λ max of the Acebrophyllline was found to be 274 nm. The method showed high sensitivity with linearity in the range of 2 to 20μg/ml, coefficient regression was found to be 0.9994. The regression of the curve was found to be Y = 0.0187X-0.0062. Percent relative standard deviation value is below 2.0 for intraday and interday precision indicated that method is highly precised. The percentage recovery value (average 100.1458 percentages), indicated the accuracy and specificity of the method. The proposed method will be suitable for the analysis of Acebrophylline in bulk and pharmaceutical formulation.
JPC - Journal of Planar Chromatography - Modern TLC
Journal of Inclusion Phenomena and Macrocyclic Chemistry, Mar 14, 2008
Abstract The physicochemical properties and dissolution profile of zaleplon (ZPN) b-cyclodextrin ... more Abstract The physicochemical properties and dissolution profile of zaleplon (ZPN) b-cyclodextrin (bCD) inclusion complex were investigated. The phase solubility profile of ZPN with b-cyclodextrin was classified as AL-type. Sta-bility constant with 1:1 molar ratio was calculated from the ...
Journal of Inclusion Phenomena and Macrocyclic Chemistry, 2008
Abstract The physicochemical properties and dissolution profile of zaleplon (ZPN) b-cyclodextrin ... more Abstract The physicochemical properties and dissolution profile of zaleplon (ZPN) b-cyclodextrin (bCD) inclusion complex were investigated. The phase solubility profile of ZPN with b-cyclodextrin was classified as AL-type. Sta-bility constant with 1:1 molar ratio was calculated from the ...
Journal of Inclusion …, 2008
The physicochemical properties and dissolution profile of zaleplon (ZPN) b-cyclodextrin (bCD) inc... more The physicochemical properties and dissolution profile of zaleplon (ZPN) b-cyclodextrin (bCD) inclusion complex were investigated. The phase solubility profile of ZPN with b-cyclodextrin was classified as AL-type. Sta-bility constant with 1:1 molar ratio was calculated from the ...
Journal of Inclusion …, 2008
The physicochemical properties and dissolution profile of zaleplon (ZPN) b-cyclodextrin (bCD) inc... more The physicochemical properties and dissolution profile of zaleplon (ZPN) b-cyclodextrin (bCD) inclusion complex were investigated. The phase solubility profile of ZPN with b-cyclodextrin was classified as AL-type. Sta-bility constant with 1:1 molar ratio was calculated from the ...
International Journal of Mass Spectrometry, Dec 31, 2023
Study reports that the, stability indicating high-performance thin layer chromatographic techniqu... more Study reports that the, stability indicating high-performance thin layer chromatographic technique (HPTLC) was developed as well as validated for estimating Paracetamol and Prochlorperazine in bulk and dosage form. These drugs are largely used in migraine and first line treatment of schizophrenia. Selected drug combination employed for Quality by design based Box Behnken design by high performance thin layer chromatography method utilizing aluminium plates; pre-coated using silica gel 60F 254 as stationary phase by using acetone: toluene: methanol (4:4:2 v/v/v) as a mobile phase. Densitogram shows R f values for Paracetamol at 0.74 and for Prochlorperazine at 0.32 evaluated by densitometry measurement bands at 248 nm. Paracetamol and Prochlorperazine marketed formulation of 700 mg by using various excipients prepared at lab scale and it was evaluated for various types of tablet tests, and it showed the accurate result with respect to standard guidelines. The approach has been validated in accordance with International Conference on Harmonization (ICH) requirements. The correlation coefficient was found to be 0.992 and 0.998 at a concentration range of 100-300 μg/ band and 1000-3000 ng/band for Paracetamol and Prochlorperazine, respectively. The method had an accuracy of 100.51 % and 100.53 % for Paracetamol and Prochlorperazine, respectively. The degradation of Paracetamol and Prochlorperazine was carried out in an acid, alkaline, oxidative, thermal and photolytic environment. The stress degraded product was successfully separated using the HPTLC and using high resonance mass spectroscopy technique (HRMS-MS), the degradation product was discovered.
BioNanoScience
Diabetes mellitus is a metabolic disorder in which a person fails to produce adequate insulin or ... more Diabetes mellitus is a metabolic disorder in which a person fails to produce adequate insulin or insulin reactions to cells, so that blood sugar levels are high in diabetes patients. Nanoparticles of small particle size have very significant effects against other types of dosage. Diabetes is treated with metal oxide-like nanoparticles such as zinc oxide, silver oxide, cerium oxide, magnesium oxide, vanadium oxide, chromium oxide, and gold nanoparticles. Different plants are used for the green synthesis of zinc, silver, magnesium oxide, cerium oxide, and golden nanoparticles. These analyses include separate in vitro and in vivo tests. The in vitro studies include nanoparticles characterization, antioxidant studies, anti-diabetic studies, and green synthesized nanoparticles phytochemical studies. In the in vivo trials, the animal research involves a range of test models, diabetes induction, experimental design, sample collection, and blood sample characterization by different testing methods. Studies in in vitro and in vivo reveal nanoparticles of metal oxide to be anti-diabetic.
Social Science Research Network, 2022
Highlights Metal Oxide (MO) nanoparticles were synthesized using a green synthetic method and c... more Highlights Metal Oxide (MO) nanoparticles were synthesized using a green synthetic method and characterized. The catalytic property of the synthesized MO nanoparticles were examined. A green protocol for the reduction of aromatic aldehydes is demonstrated.
Indian drugs, Dec 27, 2022
Reversed phase high performance liquid chromatography (RP-HPLC) method for the quantitative deter... more Reversed phase high performance liquid chromatography (RP-HPLC) method for the quantitative determination of saxagliptin (SXG) in human urine was developed and validated to support clinical studies. Chromatographic separation was achieved on an Inertsil® column (250 mm x 4.6 mm, 5 µm). Isocratic elution using a mobile phase of potassium dihydrogen phosphate buffer pH (3) - acetonitrile (80:20, V/V) at a flow rate of 1 mL min-1 with UV detection at 212.1 nm was performed. The retention time of saxagliptin was 6.4 min. The method was validated according to United State Food and Drug Administration (USFDA) (May-2001) guidelines. The developed bioanalytical method was found to be selective, accurate, precise, and having good extraction efficiency. The developed method was satisfactorily applied to the routine quality control analysis of the saxagliptin.
Study reports that the, stability indicating high-performance thin layer chromatographic techniqu... more Study reports that the, stability indicating high-performance thin layer chromatographic technique (HPTLC) was developed as well as validated for estimating Paracetamol and Prochlorperazine in bulk and dosage form. These drugs are largely used in migraine and first line treatment of schizophrenia. Selected drug combination employed for Quality by design based Box Behnken design by high performance thin layer chromatography method utilizing aluminium plates; pre-coated using silica gel 60F 254 as stationary phase by using acetone: toluene: methanol (4:4:2 v/v/v) as a mobile phase. Densitogram shows R f values for Paracetamol at 0.74 and for Prochlorperazine at 0.32 evaluated by densitometry measurement bands at 248 nm. Paracetamol and Prochlorperazine marketed formulation of 700 mg by using various excipients prepared at lab scale and it was evaluated for various types of tablet tests, and it showed the accurate result with respect to standard guidelines. The approach has been validated in accordance with International Conference on Harmonization (ICH) requirements. The correlation coefficient was found to be 0.992 and 0.998 at a concentration range of 100-300 μg/ band and 1000-3000 ng/band for Paracetamol and Prochlorperazine, respectively. The method had an accuracy of 100.51 % and 100.53 % for Paracetamol and Prochlorperazine, respectively. The degradation of Paracetamol and Prochlorperazine was carried out in an acid, alkaline, oxidative, thermal and photolytic environment. The stress degraded product was successfully separated using the HPTLC and using high resonance mass spectroscopy technique (HRMS-MS), the degradation product was discovered.
Diabetes mellitus is a metabolic disorder in which a person fails to produce adequate insulin or ... more Diabetes mellitus is a metabolic disorder in which a person fails to produce adequate insulin or insulin reactions to cells, so that blood sugar levels are high in diabetes patients. Nanoparticles of small particle size have very significant effects against other types of dosage. Diabetes is treated with metal oxide-like nanoparticles such as zinc oxide, silver oxide, cerium oxide, magnesium oxide, vanadium oxide, chromium oxide, and gold nanoparticles. Different plants are used for the green synthesis of zinc, silver, magnesium oxide, cerium oxide, and golden nanoparticles. These analyses include separate in vitro and in vivo tests. The in vitro studies include nanoparticles characterization, antioxidant studies, anti-diabetic studies, and green synthesized nanoparticles phytochemical studies. In the in vivo trials, the animal research involves a range of test models, diabetes induction, experimental design, sample collection, and blood sample characterization by different testing methods. Studies in in vitro and in vivo reveal nanoparticles of metal oxide to be anti-diabetic.
Indian Drugs
Reversed phase high performance liquid chromatography (RP-HPLC) method for the quantitative deter... more Reversed phase high performance liquid chromatography (RP-HPLC) method for the quantitative determination of saxagliptin (SXG) in human urine was developed and validated to support clinical studies. Chromatographic separation was achieved on an Inertsil® column (250 mm x 4.6 mm, 5 µm). Isocratic elution using a mobile phase of potassium dihydrogen phosphate buffer pH (3) - acetonitrile (80:20, V/V) at a flow rate of 1 mL min-1 with UV detection at 212.1 nm was performed. The retention time of saxagliptin was 6.4 min. The method was validated according to United State Food and Drug Administration (USFDA) (May-2001) guidelines. The developed bioanalytical method was found to be selective, accurate, precise, and having good extraction efficiency. The developed method was satisfactorily applied to the routine quality control analysis of the saxagliptin.
J Incl Phenom Macrocycl Chem, 2008
International Journal Of Pharmaceutical Research And Allied Sciences
Inorganic Chemistry Communications, 2022
International journal of applied research, 2018
Diabetic peripheral neuropathy is a long-term complication of diabetes. Reflexology for neuropath... more Diabetic peripheral neuropathy is a long-term complication of diabetes. Reflexology for neuropathatic pain works with the help of chemical, electrical, and nervous systems of the body – sending messages across to balance organs and parts in far reaching areas of the body. With neuropathy, reflexology has a great option of gently activating the damaged nerve fibers to send and receive correct nerve signals. Purpose: To assess the effect of foot reflexology on.neuropathic pain among diabetic patients. Objectives: To assess peripheral neuropathic pain before and after foot reflexology among diabetic patients, to compare peripheral neuropathic pain before and after foot reflexology among diabetic patients. Method: This was quantitative Quasi- experimental pre test post test control group design of 30 patients who were diabetic and had peripheral neuropathic pain, aged 31-60 years. The patients selected for study were diabetic patients who were suffering from peripheral neuropathic pain ...
INDIAN DRUGS
A simple stability indicating RP-HPLC method was validated for determination of ipratropium bromi... more A simple stability indicating RP-HPLC method was validated for determination of ipratropium bromide in the bulk drug. The drug was resolved using HPLC Column (Kromasil ODS 150 x 4.6 mm C18 column) with mobile phase of HPLC grade acetonitrile:potassium di-hydrogen phosphate buffer (60:40 V/V) at a flow rate of 1 ml/min. The retention time of ipratropium bromide was 3.7 min with UV detection at 254 nm. The method was validated with respect to linearity, sensitivity, accuracy, precision and robustness as per the International Conference on Harmonization (ICH) guidelines. The method was specific and it was observed that no interference with diluents. The linearity was established over the concentration range of 20-120 μg/ml with correlation coefficients (r2) 0.9958 for ipratropium bromide. The mean recovery was found to be in the range of 99.8% for ipratropium bromide. The % R.S.D. values for intraday precision study and inter-day study were <1.0%, confirming that the method was suff...
JPC – Journal of Planar Chromatography – Modern TLC
Journal of Current Pharma Research
A simple, sensitive and accurate spectrophotometric method has been developed for the determinati... more A simple, sensitive and accurate spectrophotometric method has been developed for the determination of Acebrophylline in bulk drug and capsule. The λ max of the Acebrophyllline was found to be 274 nm. The method showed high sensitivity with linearity in the range of 2 to 20μg/ml, coefficient regression was found to be 0.9994. The regression of the curve was found to be Y = 0.0187X-0.0062. Percent relative standard deviation value is below 2.0 for intraday and interday precision indicated that method is highly precised. The percentage recovery value (average 100.1458 percentages), indicated the accuracy and specificity of the method. The proposed method will be suitable for the analysis of Acebrophylline in bulk and pharmaceutical formulation.
JPC - Journal of Planar Chromatography - Modern TLC
Journal of Inclusion Phenomena and Macrocyclic Chemistry, Mar 14, 2008
Abstract The physicochemical properties and dissolution profile of zaleplon (ZPN) b-cyclodextrin ... more Abstract The physicochemical properties and dissolution profile of zaleplon (ZPN) b-cyclodextrin (bCD) inclusion complex were investigated. The phase solubility profile of ZPN with b-cyclodextrin was classified as AL-type. Sta-bility constant with 1:1 molar ratio was calculated from the ...
Journal of Inclusion Phenomena and Macrocyclic Chemistry, 2008
Abstract The physicochemical properties and dissolution profile of zaleplon (ZPN) b-cyclodextrin ... more Abstract The physicochemical properties and dissolution profile of zaleplon (ZPN) b-cyclodextrin (bCD) inclusion complex were investigated. The phase solubility profile of ZPN with b-cyclodextrin was classified as AL-type. Sta-bility constant with 1:1 molar ratio was calculated from the ...
Journal of Inclusion …, 2008
The physicochemical properties and dissolution profile of zaleplon (ZPN) b-cyclodextrin (bCD) inc... more The physicochemical properties and dissolution profile of zaleplon (ZPN) b-cyclodextrin (bCD) inclusion complex were investigated. The phase solubility profile of ZPN with b-cyclodextrin was classified as AL-type. Sta-bility constant with 1:1 molar ratio was calculated from the ...
Journal of Inclusion …, 2008
The physicochemical properties and dissolution profile of zaleplon (ZPN) b-cyclodextrin (bCD) inc... more The physicochemical properties and dissolution profile of zaleplon (ZPN) b-cyclodextrin (bCD) inclusion complex were investigated. The phase solubility profile of ZPN with b-cyclodextrin was classified as AL-type. Sta-bility constant with 1:1 molar ratio was calculated from the ...