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SeeDB-Live has been commercialized from Nacalai Tesque!
SeeDB-Live paper: Inagaki et al., Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo. Natue Methods (2026)
The paper was fertured by The Transmitter and FocalPlane.
Post-hoc correction of chromatic aberrations in cleared samples. Leiwe MN, Fujimoto S, Imai T. Post hoc Correction of Chromatic Aberrations in Large-Scale Volumetric Images in Confocal Microscopy. Front Neuroanat (2021)
The Tetbow image is now in a science museum, Miraikan, in Tokyo. See the image.
Tetbow image was featured as The Most Stunning Neuroscience Image 2018 (by Forbes) and The Photo of the Year 2018 (The Scientist).
Addgene blog post on Tetbow was published.
SeeDB and SeeDB2 have been commercialized from FUJIFILM Wako Pure Chemical: SeeDB Kit and SeeDB2 Kit.

Video 17.wmv

The first-generation immersion-based clearing agent for morphological analyses of neurons.

An immersion-based clearing agent for high-resolution imaging.

Bright Brainbow-like multicolor labeling with up to 7 fluorescent proteins. Plasmids and AAV vectors are available.

Post-hoc correction programs for chromatic aberrations in cleared samples.

Image J/Fiji plugin for channel unmixing of fluorescent signals.

Automated neuronal reconstruction using super-multicolor Tetbow and hue-based neurite identification.

Optical clearing media for live tissues.

Fluorescent barcode vectors

AAV vectors for 21-plex circuit tracing.

>> Moriyasu et al., unpublished

Please contact us for reagents and collaborations.

Contact:

Takeshi Imai (Principal Investigator)

email: imai.takeshi.457[at]m.kyushu-u.ac.jp

Graduate School of Medical Sciences, Kyushu University

Since 6/24/2013

last updated: 3/12/2026