syed mudassar | Sher-i-Kashmir Institue Of Medical Sciences,soura,srinagar-190011,india (original) (raw)

Papers by syed mudassar

Journal of Global Infectious Diseases, 2018

Background: In hepatitis C virus (HCV), infection viral and IL28B genotype along with many clinic... more Background: In hepatitis C virus (HCV), infection viral and IL28B genotype along with many clinical and biochemical factors can influence response rates to pegylated interferon plus ribavirin (Peg-IFN-a/R) therapy and progression to chronic hepatitis C (CHC). Aims: The present study was conducted to determine the effect of biochemical and risk factors on treatment outcome in CHC patients in relation to their viral and host genotype. Settings and Design: The present study was a prospective Pe- IFN efficacy study consisting of Peg-IFN-a/R therapy for 24–48 weeks including 250 HCV infected patients. Materials and Methods: Biochemical parameters were determined by Beckman Coulter AU680 automated analyzer. HCV and Interleukin 28B (IL28B) genotyping were carried out by polymerase chain reaction-restriction fragment length polymorphism and viral load was determined by quantitative real-time PCR. Results: Wild outnumbered the variant genotypes in rs 12979860, rs 12980275, and rs 8099917 SNP of IL28B gene. Sustained virological response (SVR) SVR and viral genotype were significantly associated with age, hepatic steatosis, low-grade varices, and serum aspartate transaminase levels (at the end of treatment) (P < 0.05). In addition, SVR was significantly influenced by body mass index (BMI), insulin resistance, serum low-density lipoprotein , and ferritin levels (P < 0.05). Viral genotype 1 infected patients had higher serum cholesterol and triglyceride levels (P < 0.05). Conclusions: Although the IL28B sequence variation is the major factor that can influence response rates to antiviral therapy, viral and biochemical factors also have a definite role to play in the diagnosis, etiology, and treatment outcome in HCV-infected patients.

Indian Journal of Endocrinology and Metabolism, 2023

Clinical and Experimental Medicine, Nov 1, 2022

International Journal of Research in Medical Sciences, Jul 25, 2019

Background: Pregnancy is a stressful condition accompanied by a high energy demand and increased ... more Background: Pregnancy is a stressful condition accompanied by a high energy demand and increased oxygen requirement. Oxidative stress has been recognized as a significant factor linked to hypertension. Elucidation of antioxidant cascade in patients with pregnancy induced hypertension (PIH). can give insights about the oxidative stress and lead to better management of the condition. It was a prospective case control study to elucidate the parameters of oxidative stress in patients with PIH. Methods: Levels of Malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) were eludidated using enzyme linked immunosorbent assay (ELISA) in hypertensive mothers and their age matched pregnant and non-pregnant controls to determine the lipid peroxidation and oxidative stress. Results: A total of four hundred and twenty study subjects were enrolled in the study. Malondialdehyde levels from mothers with hypertension were significantly higher than their age matched pregnant controls. The results indicate that oxidative stress induced by pregnancy induced hypertension manifests as increased lipid peroxidation. Conclusion: There is a decrement in anti-oxidant status reflecting the ineffective scavenging of reactive oxygen species resulting in oxidative damage and tissue injury.

Journal of Medical Sciences, Dec 12, 2013

LAP LAMBERT Academic Publishing eBooks, Oct 18, 2016

Thyroid carcinoma is the most prevalent endocrine malignancy and accounts for 2% of all human can... more Thyroid carcinoma is the most prevalent endocrine malignancy and accounts for 2% of all human cancers. We screened exon 15 of BRAF gene; exons 1 and 2 of RAS genes in sixty (60) consecutive thyroid tissue (tumor and adjacent normal) samples. Overall mutations in BRAF was found to be 25% (15 of 60) affecting codon 600 (valine to glutamine) and restricted only to papillary thyroid cancer but devoid of any RAS mutation. We screened 140 blood samples from thyroid cancer patients for HRAS T81C and RET polymorphism in comparison with 170 cancer-free controls. TSHR gene was found to be hyper methylated in 25 % (15 of 60) of the cases with strong association with elevated TSH levels. We conclude that both mutational events as well as over-expression of BRAF gene is highly implicated in pathogenesis of thyroid cancer and the BRAF protein overexpression is independent of the BRAF mutational status. HRAS T81C and RET polymorphisms moderately increases thyroid cancer risk. Our study showed a high implication of TSHR gene methylation and its signiﬁcant association with BRAF V600E mutation in thyroid tumors, depicting a positive connection between TSHR pathway and MAP Kinase pathway.

Journal of Bioengineering and Biomedical Science, 2015

Mass spectrometry (MS) has become a powerful technology in the discovery and development of prote... more Mass spectrometry (MS) has become a powerful technology in the discovery and development of protein therapeutics in the biopharmaceutical industry. This review article describes the two main types of mass spectrometry methods for determining the protein structure. Further the role of tandem mass spectrometry in diagnostics has also been highlighted. Mass spectrometry based proteomics is a central life science technology that has realized great progress towards identification, quantification and characterization of the proteins that constitute a proteome.

Clinical and Experimental Medicine, Dec 20, 2022

CRC Press eBooks, Oct 26, 2022

Book Publisher International (a part of SCIENCEDOMAIN International), Aug 24, 2022

Acta Scientific Nutritional Health, May 1, 2022

Objectives: Inadequate iodine intake has a profound adverse impact on a child's developmental beh... more Objectives: Inadequate iodine intake has a profound adverse impact on a child's developmental behaviour. The study's aim was to ascertain the median urinary iodine concentration (mUIC) and the prevalence of iodine deficiency, as well as to understand whether maternal characteristics have an effect on iodine status in Kashmiri pregnant women. Study Design: Prospective study Methods: A total of 112 pregnant women subjects were enrolled in this study from the Department of gynaecology and obstetrics, Sher-I-Kashmir Institute of Medical Sciences (SKIMS), Srinagar. UIC was calculated using a modified Sandell-Kolthoff reaction. The World Health Organization classification was established as a suggested cut off value of 150 µg/L to categorise the iodine status of pregnant women. Results: All the 112 recruited subjects in their first trimester, were initially examined for goitre status. The mUIC was 80.91 (interquartile range (IQR): 49.2-116.47)μg/L. In our study 80 (71.4%) study subjects were from rural areas. 101 pregnant women (90%) had UIC < 150µg/L (insufficient), 8(7.14%) had UIC of 150-250µg/L(normal) and only 3(2.67%) study subjects had UIC of > 250µg/ L(excess). Significantly higher UIC was present in the younger population having age ≤ 25 years (104 µg/L), as well as in urban subjects (117.38µg/ L) and in subjects without goitre (87.85µg/ L). The goitre prevalence was 37.5%. It was 7-fold more common in illiterate subjects as compared to literate (OR = 7.14, 95%CI:2.94-17.37). Similarly rural pregnant women were at 2-fold more risk than urban for goitre occurrence (OR = 2.39,95%CI:1.03-5.51). Likewise, income status of the study participants was significantly associated with UIC (p < 0.027). UIC also showed a weak negative correlation with age (r =-.265, p = 0.005) Conclusion: This research concludes that there is a mild to moderate iodine deficiency in pregnant mothers and thus a broad and representative sample of Kashmiri pregnant women is needed to assess iodine status at the population level.

Journal of Infection in Developing Countries, Sep 30, 2018

Introduction: Viral genotype and variation in host genes involved in the immune response may pred... more Introduction: Viral genotype and variation in host genes involved in the immune response may predict the treatment response in patients infected with HCV. The present study was designed to determine the distribution pattern of HCV and host genotypes in Chronic Hepatitis C (CHC) patients and their association with virological response and other risk factors. Methodology: Two hundred and fifty (n = 250) HCV positive patients were included in the study. HCV and Interleukin 28B (IL28B) genotyping was carried out by PCR-RFLP. Results: Viral genotype 3 was the predominant genotype seen in 187 (74.8%) patients. Wild genotype predominated in rs12979860, rs12980275 and rs8099917 SNP of IL28B gene. A significant difference was found in end stage virological response (EVR) between HCV genotype 1 infected patients with wild and variant genotype for rs12980275 and rs8099917 SNPs respectively (P < 0.05). On multivariate analysis all the SNPs were found to be associated with each other (P < 0.05) with rs12980275 SNP associated with history of Jaundice (P < 0.05). Viral genotype 3 was significantly associated with age (< 50 years) and rapid virological response (RVR) while as viral genotype 1 was significantly associated with history of surgery on multivariate analysis (P < 0.05). Conclusions: The viral genotype and IL28B polymorphisms are important factors to personalize antiviral therapy of patients with CHC.

Daehan imsang geomsa haghoeji, Sep 30, 2022

In clinical practice, the diagnosis of tuberculosis (TB) continues to be a challenge. The goal of... more In clinical practice, the diagnosis of tuberculosis (TB) continues to be a challenge. The goal of this study was to evaluate the reliability and impact of adenosine deaminase (ADA) enzyme testing as a biochemical marker in the continued management of suspected tuberculosis in a limited resource setting hospital. The retrospective data were collected from 2018 to 2021 and comprised the results of all ADA test assays done in the laboratory. All types of body fluids received for ADA testing were analyzed. Over the course of two years, 1461 samples for ADA assay testing were received. The average age of the study population was 56.69±11.7 years, with males accounting for the majority of the subjects (55.72%). Pleural fluid (N=817, 55.92%) was the most common type of sample received for the ADA assay. 114 (13.95%) of the 817 pleural fluid samples were found to be positive. A survey was conducted to obtain physician's response regarding reliability on ADA testing. 100% of them reported the supportive role of ADA levels in the workup of patients with suspected tuberculosis. In a limited resource setting, the ADA test, in conjunction with clinical and other laboratory findings, can help physicians to initiate early treatment in hospitals for the benefit of patients.

Journal of family medicine and primary care, 2022

Clinical Medicine and Diagnostics, 2019

Gestational hypertension (GH) or pregnancy induced hypertension (PIH) is a condition characterise... more Gestational hypertension (GH) or pregnancy induced hypertension (PIH) is a condition characterised by high blood pressure during pregnancy. PIH can lead to serious condition called Pre-Eclampsia or Toxemia. The condition prevents placenta from getting enough blood resulting in low birth weight. Present study is a prospective case control study elucidating the effect of various demographic, clinicopathological and obstetric parameters on PIH. A total of 120 females with PIH and 150 normotensive pregnant females were included in the study. The survey was conducted using an interviewer administered questionnaire. The mean systolic and diastolic Blood Pressure (BP) of subjects with PIH was found to be 148.2 ± 5.5 and 96.2 ± 5.8 respectively. PIH was found to be statistically associated with occupation, socioeconomic status, history of PIH, previous miscarriage, family history of hypertension and diabetes (P≤0.05). The results of the present study clearly pinpoint the cluster of factors that are associated with gestational hypertension. Identifying these factors may provide a window to clinicians and help them to recognize mothers who have higher chances to develop hypertensive disorders.

Journal of clinical and experimental hepatology, Jun 1, 2015

Allergy, Asthma & Clinical Immunology, Nov 23, 2021

Objectives: Increased levels of serum Immunoglobulin-E (IgE) and different genetic variants of cy... more Objectives: Increased levels of serum Immunoglobulin-E (IgE) and different genetic variants of cytokines are common biochemical manifestation in Allergy. The current study was aimed to study the association of IgE and different variants of Interleukin-4 (IL-4), and Interleukin-13 (IL-13) genes with different kind of allergies. Methods: A pre-tested questionnaire was used to collect all the dietary, life style and clinical details by a trained staff. A blood sample of 2 ml each was collected in coagulated and anti-coagulated vials. DNA and serum samples were extracted and stored until further use. Serum IgE were estimated by ELISA while as the genotypic analysis was done by PCR-RFLP methods. Results: Statistically a significant difference of serum IgE levels were observed among cases and controls (P < 0.05). The observed significant difference of serum IgE levels were retained among subjects who also harboured variant genotypes of IL-4 and IL-13 genes (P < 0.05). Additionally, the above genetic variants significantly modified the risk of allergy when stratification was done based on various clinical characteristics. Conclusion: Our study suggests that increased IgE levels and in association with variant forms of IL-4 and IL-13 genes are significantly associated with different types of allergies in study population.

Clinical Immunology, Aug 1, 2021

INTRODUCTION IL4 pathway is known to upregulate IgE mediated immune responses and responsible for... more INTRODUCTION IL4 pathway is known to upregulate IgE mediated immune responses and responsible for the manifestation of Atopic disorders. The current study was aimed to elucidate the genetic variations of Interleukin 4 (IL4) and Interleukin 4 receptor alpha (IL4R) genes and their possible association with atopic subjects. METHODS The well-designed questionnaire was used to collect the subject demographic and clinical details. Biochemical parameters were analysed using Chemiluminescent Immunoassay (CLIA) technique. The genotyping was performed using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). RESULTS We observed a statistically significant difference of serum Immunoglobulin-E (IgE) levels among cases and controls (P<0.05). Subjects harbouring the variant genotypes of I50V and Q576R single nucleotide polymorphisms (SNPs) in IL4R gene showed statistically differential risk towards atopic disorders. However, the variants genotype of 70 bp VNTR polymorphism in IL4 gene showed a protective role towards in predisposition to Atopy. On stratification, the above genetic variants had a significant impact on modifiable and non-modifiable factors associated with the disease. CONCLUSION Our study demonstrates that increased IgE levels and IL4 gene variants (I50V and Q576R) are significantly associated towards predisposition to allergic disorders in this study population.

Pathology & Oncology Research, Apr 22, 2017

Large tumor suppressor (LATS) is an important member of the Hippo pathway which can regulate orga... more Large tumor suppressor (LATS) is an important member of the Hippo pathway which can regulate organ size and cell proliferation. However, very little is known about the expression and clinical significance of LATS in lung cancer especially from this part of the world. We elucidated the frequency of LATS1 &LATS2 promoter hypermethylation (by methylation-specific PCR) and expression (by real-time PCR) in sixty nine (n = 69) Non-Small Cell Lung Cancer (NSCLC) patients and their corresponding normal lung tissue samples. We found promoter hypermethylation frequencies of LATS1 & LATS1to be 66.66% (46/69) and 71% (49/69) in NSCLC tissues. Decreased LATS1 & LATS2 mRNA expression was found in 55% and 66.66% of NSCLC patients. The LATS1 mRNA expression was significantly higher in normal lung tissues. Also, the mRNA levels of LATS1 and LATS2 NSCLC tissues with hypermethylation were significantly lower. Multivariable analysis confirmed that LATS1 under expression increased the hazard of death after adjusting for other clinicopathological factors. Importantly, the loss of LATS1 mRNA expression was associated with overall short survival. LATS1 is an independent prognostic factor and may play an important role in NSCLC progression and may serve as a novel therapeutic target of NSCLC.

Journal of Cancer Research and Therapeutics