Dr. Mohsina Mushtaq | SKUAST-KASHMIR (original) (raw)
Papers by Dr. Mohsina Mushtaq
Indian Journal of Animal Sciences, Oct 5, 2021
Applied Veterinary Research, 2022
Indian Journal of Veterinary Research (The), 2019
College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, 2020
The present study was conducted to assess the effect of levamisole therapy on udder health of dai... more The present study was conducted to assess the effect of levamisole therapy on udder health of dairy cows. Cows in the levamisole group were administered Lemasol-75®@ 2.5mg/kg b.w., S/C, once weekly at day-35,-28,-21, and-14 prior to the expected calving date while cows in control group were not given any treatment. Results revealed lower incidence of subclinical mastitis in the levamisole group compared to the control group. The somatic cell count and standard plate count levels in the levamisole group were significantly (P<0.05) lower as compared to those in the control group. Daily milk yield in levamisole group was significantly (P<0.05) higher compared to the control group. Conclusively, levamisole administration during late dry period improves the early lactation udder health of dairy cows.
The present study was carried out to evaluate the effect of mineral supplementation on milk yield... more The present study was carried out to evaluate the effect of mineral supplementation on milk yield and milk quality of dairy animals in Sub-mountainous zone of Punjab. Area specific mineral mixture was fed to lactating cattle and buffaloes of Hoshiarpur and Nawanshahar districts for 3 months at a constant dose rate of 50 g/animal/day and its effect on milk yield and its quality was analyzed at 3rd month post-treatment (last day of feeding of mineral mixture) and one month after cessation of the feeding of mineral mixture. Supplemented animals showed significant increase in milk yield as compared to un-supplemented group of animals, whereas, no significant difference was observed in milk components between the supplemented and un-supplemented groups.
The study was undertaken in Holstein Friesian crossbred dairy cows (n=16) in late gestation. The ... more The study was undertaken in Holstein Friesian crossbred dairy cows (n=16) in late gestation. The cows were divided into two equal groups of eight animals each. Group-1 was administered with four doses of Levamisole on 35, 28, 21 and 14th day prior to parturition and Group-2 was control. Colostrum and blood samples were collected from both groups on calving day for colostrum and calving day as well as 28th day in milking for blood and analysed. Evaluation revealed significantly (P<0.05) lower plasma Malondialdehyde (MDA) levels and higher Glutathione peroxidase (GPx), Glutathione S transferase (GST) and Superoxide dismutase enzyme (SOD) activity in Levamisole treated group as compared to control group. Serum and colostrum total Ig levels in Levamisole treated groups were significantly (P<0.05) higher as compared to control groups. The study concluded that Levamisole therapy during late dry period has positive effect on immunity in dairy cows.
Haryana Veterinarian, 2020
The study was undertaken in Holstein Friesian crossbred dairy cows (n=16) in late gestation. The ... more The study was undertaken in Holstein Friesian crossbred dairy cows (n=16) in late gestation. The cows were divided into two equal groups of eight animals each. Group-1 was administered with four doses of Levamisole on 35, 28, 21 and 14th day prior to parturition and Group-2 was control. Colostrum and blood samples were collected from both groups on calving day for colostrum and calving day as well as 28th day in milking for blood and analysed. Evaluation revealed significantly (P<0.05) lower plasma Malondialdehyde (MDA) levels and higher Glutathione peroxidase (GPx), Glutathione S transferase (GST) and Superoxide dismutase enzyme (SOD) activity in Levamisole treated group as compared to control group. Serum and colostrum total Ig levels in Levamisole treated groups were significantly (P<0.05) higher as compared to control groups. The study concluded that Levamisole therapy during late dry period has positive effect on immunity in dairy cows.
International journal of scientific research in science, engineering and technology, 2016
In this paper we analysed the signals of Gastrocnemius (GST) and Soleus (SOL) calf muscles from a... more In this paper we analysed the signals of Gastrocnemius (GST) and Soleus (SOL) calf muscles from a unilateral clubfoot patient (right leg normal and left clubfoot) during different stages (resting, flexion and extension) using EMG. The Raw and filtered [Root Mean Square (RMS) and Integrated EMG (IEMG)] signals acquired from the subject were quantified using (AcqKnowledge 3.9, BIOPAC MP-100 systems Inc.), for 120 seconds and the output was analysed using paired sample T-test. Significant differences were observed between the Root Mean Square (RMS) EMG and Integrated EMG (IEMG) muscle activity of normal and clubfoot leg during the three conditions for both GST and SOL muscles. With the mathematical modelling tools, the results were studied and was found that the calf muscle activity of affected leg varies from the normal leg during resting, flexion and extension stages. It was also studied and proved that relapses can occur even after surgical correction (Achilles tenotomy) on these pa...
Haryana Veterinarian, 2020
Kandi area, the sub-mountainous zone, is located in northeastern parts of Gurdaspur, Hoshiarpur, ... more Kandi area, the sub-mountainous zone, is located in northeastern parts of Gurdaspur, Hoshiarpur, Nawanshahar, Ropar and Patiala districts and comprises of about 9 percent of the total geographical area of Punjab. This zone is economically weaker region of the state. There is an acute shortage of forages in Kandi area and animals are underfed in terms of macronutrients and micronutrients (Hundal et al., 2009). Livestock depends on forages for their mineral requirements, which are deficient in several minerals as they grow on deficient soils (Sharma et al., 2005). Due to their increased requirement for lactation, dairy cows are more prone to mineral deficiencies (Mc Dowell et al., 1983). The deficiency of certain minerals like calcium (Ca), inorganic phosphorus (Pi), copper (Cu), and zinc (Zn) in dairy cattle has been reported under field conditions and the supplementation of area specific mineral mixture (ASMM) in improving productive and reproductive efficiency is gaining importance (Gowda et al., 2008). Keeping above things into consideration, the present study was conceptualized to assess the effect of area specific mineral mixture supplementation on plasma mineral profile and hematological parameters of dairy animals of sub-mountainous zone (Kandi area) of Punjab in relation to the mineral status of soil and fodder of the area. MATERIALS AND METHODS Experimental design: 100 dairy animals (32 cattle and 68 buffaloes) were randomly selected from the surveyed group of 200 dairy animals (76 cattle and 124 buffaloes) for the therapeutic trial and fed area specific mineral mixture @ 50g/animal/day for 3 months since the animals of Kandi area were deficit of micro and macro minerals as per the previous surveys conducted by other researchers (Singh, 2002 and Hundal et al., 2009) in this region. 32 animals (15 cattle and 17 buffaloes) were kept as control. Fodder (25) and soil (27) samples were also collected simultaneously from all the dairy units. These animals were grouped on the basis of age as group I (1-4 years), group II (5-7 years of age) and group III (>7 years). Tests and procedures: About 20 ml of blood sample was collected from each selected animal in heparinised mineral free glass vials. The samples were centrifuged @ 3000 rpm for 10-15 minutes immediately after collection and the o separated plasma samples were stored at-10 C temperature in deep freezer for subsequent mineral analysis. Also 2 ml of blood was collected in sterile plastic vials, containing disodium ethylene di-amine tetra acetic acid (Na EDTA) as anticoagulant, for estimation of 2 hematological parameters. Samples were collected on days 0 and 90 after feeding and one month after the cessation of mineral supplementation i.e. on day 120. Fodder and soil samples were collected following standard procedures and were packaged in plastic bags of suitable size for further processing. Hematology: Hemoglobin (Hb) was estimated by Drabkin's / cyanomethaemoglobin method and packed cell volume (PCV) by microhematocrit method. Hb and PCV were sufficient to know the anaemic state of the animals and less time consuming. Mineral analysis: For Ca and Mg estimation, samples were diluted with 0.1 per cent lanthanum chloride and
Haryana Veterinarian, 2020
REPORT-CUCURBIT GENETICS …, 1999
Methods. Plant material'Perlita'melon were grown singly in pots in a greenhouse using a... more Methods. Plant material'Perlita'melon were grown singly in pots in a greenhouse using a trellis system. Hermaphroditic flowers were pollinated and tagged at anthesis, with one to two fruits per plant were allowed to develop. Unripe fruit were harvested at 20, 30, and 40 ...
Haryana Veterinarian, 2018
The study was conducted to assess the mineral status of dairy animals of Kandi belt of Punjab. Bl... more The study was conducted to assess the mineral status of dairy animals of Kandi belt of Punjab. Blood samples were collected from 200 dairy animals (76 cattle and 124 buffaloes) randomly selected from 9 villages of 4 blocks of the 2 districts (Hoshiarpur and Nawanshahar). Fodder (25) and soil (27) samples were also collected simultaneously from all the dairy units. These animals were grouped on the basis of age as group I (1
Haryana Veterinarian, 2020
The present study was conducted to assess the effect of levamisole therapy on udder health of dai... more The present study was conducted to assess the effect of levamisole therapy on udder health of dairy cows. Cows in the levamisole group were administered Lemasol-75®@ 2.5mg/kg b.w., S/C, once weekly at day-35,-28,-21, and-14 prior to the expected calving date while cows in control group were not given any treatment. Results revealed lower incidence of subclinical mastitis in the levamisole group compared to the control group. The somatic cell count and standard plate count levels in the levamisole group were significantly (P<0.05) lower as compared to those in the control group. Daily milk yield in levamisole group was significantly (P<0.05) higher compared to the control group. Conclusively, levamisole administration during late dry period improves the early lactation udder health of dairy cows.
Ageing is the time related deterioration of the physiological functions, leading to the cell's in... more Ageing is the time related deterioration of the physiological functions, leading to the cell's inability to withstand external and internal stress. The causative factors for the time dependant deleterious process of ageing are yet not well defined and no single adequate molecular explanation for ageing is currently available (Singh et al., 2009). Increased oxidative stress and its definitive role in the age-related diseases is well documented fact in human beings (Pandey and Rizvi, 2010). One of the most popular explanations of how ageing occurs at the molecular level is the oxidative stress hypothesis. Free radicals are highly reactive molecules that are produced during normal metabolism in the body or after exposure to environmental pro-oxidants. Excess free radicals cause a dangerous chain reaction that can destroy nucleic acid, proteins, lipids and other cellular compounds (Haliwell and Gutteridge, 1999).
In this paper we analysed the signals of Gastrocnemius (GST) and Soleus (SOL) calf muscles from a... more In this paper we analysed the signals of Gastrocnemius (GST) and Soleus (SOL) calf muscles from a unilateral clubfoot patient (right leg normal and left clubfoot) during different stages (resting, flexion and extension) using EMG. The Raw and filtered [Root Mean Square (RMS) and Integrated EMG (IEMG)] signals acquired from the subject were quantified using (AcqKnowledge 3.9, BIOPAC MP-100 systems Inc.), for 120 seconds and the output was analysed using paired sample T-test. Significant differences were observed between the Root Mean Square (RMS) EMG and Integrated EMG (IEMG) muscle activity of normal and clubfoot leg during the three conditions for both GST and SOL muscles. With the mathematical modelling tools, the results were studied and was found that the calf muscle activity of affected leg varies from the normal leg during resting, flexion and extension stages. It was also studied and proved that relapses can occur even after surgical correction (Achilles tenotomy) on these patients with the accuracy of 80%.
The study was undertaken in Holstein Friesian crossbred dairy cows (n=16) in late gestation. The ... more The study was undertaken in Holstein Friesian crossbred dairy cows (n=16) in late gestation. The cows were divided into two equal groups of eight animals each. Group-1 was administered with four doses of Levamisole on 35, 28, 21 and 14 th day prior to parturition and Group-2 was control. Colostrum and blood samples were collected from both groups on calving day for colostrum and calving day as well as 28 th day in milking for blood and analysed. Evaluation revealed significantly (P<0.05) lower plasma Malondialdehyde (MDA) levels and higher Glutathione peroxidase (GPx), Glutathione S transferase (GST) and Superoxide dismutase enzyme (SOD) activity in Levamisole treated group as compared to control group. Serum and colostrum total Ig levels in Levamisole treated groups were significantly (P<0.05) higher as compared to control groups. The study concluded that Levamisole therapy during late dry period has positive effect on immunity in dairy cows.
The study evaluated the efficacy of multienzyme therapy as an adjunct or alternative to antibioti... more The study evaluated the efficacy of multienzyme therapy as an adjunct or alternative to antibiotic therapy in mastitic cows. Twenty one cows affected with clinical mastitis were selected and divided into three treatment groups: Group 1 (n=7) animals were treated with enrofloxacin @ 10 mg/kg b.w, I/M for 5 days. Group 2 (n=7) cows received an intramammary infusion consisting of a mixture of three enzymes viz. chymotrypsin (8 mg), trypsin (8 mg) and papain (4 mg) dissolved in 5 ml NS, at 24 hr. interval for 7 days. Group 3 (n=7) cows received a combination of both parental antibiotic (Enrocin) and intramammary infusion of multienzymes for 7 days at 24 hours interval. Milk samples were collected prior to the treatment (day 1) and on days 4, 7 and 14 post treatment and blood samples on day 1, 7 and 14. There was significant (P<0.05) decrease in the mean serum total protein (TP) and globulin levels in all the treatment groups post treatment as compared to the pretreatment levels while as the mean serum albumin levels and the A: G ratio showed significant (P<0.05) increase post treatment. There was a significant (P<0.05) decrease in the mean total sialic acid (TSA) levels on days 7 and 14 after treatment as compared to the pre-treatment levels in all the three groups. Mean somatic cell count (SCC), standard plate count (SPC) and electrical conductivity (EC) values decreased significantly on days 4, 7 and 14 as compared to that of the day 1. It was concluded that therapeutic intervention with parenteral enrofloxacin and intramammary infusion of proteolytic enzymes improved the udder health of dairy cows affected with clinicalmastitis.
Indian Journal of Animal Sciences, Oct 5, 2021
Applied Veterinary Research, 2022
Indian Journal of Veterinary Research (The), 2019
College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, 2020
The present study was conducted to assess the effect of levamisole therapy on udder health of dai... more The present study was conducted to assess the effect of levamisole therapy on udder health of dairy cows. Cows in the levamisole group were administered Lemasol-75®@ 2.5mg/kg b.w., S/C, once weekly at day-35,-28,-21, and-14 prior to the expected calving date while cows in control group were not given any treatment. Results revealed lower incidence of subclinical mastitis in the levamisole group compared to the control group. The somatic cell count and standard plate count levels in the levamisole group were significantly (P<0.05) lower as compared to those in the control group. Daily milk yield in levamisole group was significantly (P<0.05) higher compared to the control group. Conclusively, levamisole administration during late dry period improves the early lactation udder health of dairy cows.
The present study was carried out to evaluate the effect of mineral supplementation on milk yield... more The present study was carried out to evaluate the effect of mineral supplementation on milk yield and milk quality of dairy animals in Sub-mountainous zone of Punjab. Area specific mineral mixture was fed to lactating cattle and buffaloes of Hoshiarpur and Nawanshahar districts for 3 months at a constant dose rate of 50 g/animal/day and its effect on milk yield and its quality was analyzed at 3rd month post-treatment (last day of feeding of mineral mixture) and one month after cessation of the feeding of mineral mixture. Supplemented animals showed significant increase in milk yield as compared to un-supplemented group of animals, whereas, no significant difference was observed in milk components between the supplemented and un-supplemented groups.
The study was undertaken in Holstein Friesian crossbred dairy cows (n=16) in late gestation. The ... more The study was undertaken in Holstein Friesian crossbred dairy cows (n=16) in late gestation. The cows were divided into two equal groups of eight animals each. Group-1 was administered with four doses of Levamisole on 35, 28, 21 and 14th day prior to parturition and Group-2 was control. Colostrum and blood samples were collected from both groups on calving day for colostrum and calving day as well as 28th day in milking for blood and analysed. Evaluation revealed significantly (P<0.05) lower plasma Malondialdehyde (MDA) levels and higher Glutathione peroxidase (GPx), Glutathione S transferase (GST) and Superoxide dismutase enzyme (SOD) activity in Levamisole treated group as compared to control group. Serum and colostrum total Ig levels in Levamisole treated groups were significantly (P<0.05) higher as compared to control groups. The study concluded that Levamisole therapy during late dry period has positive effect on immunity in dairy cows.
Haryana Veterinarian, 2020
The study was undertaken in Holstein Friesian crossbred dairy cows (n=16) in late gestation. The ... more The study was undertaken in Holstein Friesian crossbred dairy cows (n=16) in late gestation. The cows were divided into two equal groups of eight animals each. Group-1 was administered with four doses of Levamisole on 35, 28, 21 and 14th day prior to parturition and Group-2 was control. Colostrum and blood samples were collected from both groups on calving day for colostrum and calving day as well as 28th day in milking for blood and analysed. Evaluation revealed significantly (P<0.05) lower plasma Malondialdehyde (MDA) levels and higher Glutathione peroxidase (GPx), Glutathione S transferase (GST) and Superoxide dismutase enzyme (SOD) activity in Levamisole treated group as compared to control group. Serum and colostrum total Ig levels in Levamisole treated groups were significantly (P<0.05) higher as compared to control groups. The study concluded that Levamisole therapy during late dry period has positive effect on immunity in dairy cows.
International journal of scientific research in science, engineering and technology, 2016
In this paper we analysed the signals of Gastrocnemius (GST) and Soleus (SOL) calf muscles from a... more In this paper we analysed the signals of Gastrocnemius (GST) and Soleus (SOL) calf muscles from a unilateral clubfoot patient (right leg normal and left clubfoot) during different stages (resting, flexion and extension) using EMG. The Raw and filtered [Root Mean Square (RMS) and Integrated EMG (IEMG)] signals acquired from the subject were quantified using (AcqKnowledge 3.9, BIOPAC MP-100 systems Inc.), for 120 seconds and the output was analysed using paired sample T-test. Significant differences were observed between the Root Mean Square (RMS) EMG and Integrated EMG (IEMG) muscle activity of normal and clubfoot leg during the three conditions for both GST and SOL muscles. With the mathematical modelling tools, the results were studied and was found that the calf muscle activity of affected leg varies from the normal leg during resting, flexion and extension stages. It was also studied and proved that relapses can occur even after surgical correction (Achilles tenotomy) on these pa...
Haryana Veterinarian, 2020
Kandi area, the sub-mountainous zone, is located in northeastern parts of Gurdaspur, Hoshiarpur, ... more Kandi area, the sub-mountainous zone, is located in northeastern parts of Gurdaspur, Hoshiarpur, Nawanshahar, Ropar and Patiala districts and comprises of about 9 percent of the total geographical area of Punjab. This zone is economically weaker region of the state. There is an acute shortage of forages in Kandi area and animals are underfed in terms of macronutrients and micronutrients (Hundal et al., 2009). Livestock depends on forages for their mineral requirements, which are deficient in several minerals as they grow on deficient soils (Sharma et al., 2005). Due to their increased requirement for lactation, dairy cows are more prone to mineral deficiencies (Mc Dowell et al., 1983). The deficiency of certain minerals like calcium (Ca), inorganic phosphorus (Pi), copper (Cu), and zinc (Zn) in dairy cattle has been reported under field conditions and the supplementation of area specific mineral mixture (ASMM) in improving productive and reproductive efficiency is gaining importance (Gowda et al., 2008). Keeping above things into consideration, the present study was conceptualized to assess the effect of area specific mineral mixture supplementation on plasma mineral profile and hematological parameters of dairy animals of sub-mountainous zone (Kandi area) of Punjab in relation to the mineral status of soil and fodder of the area. MATERIALS AND METHODS Experimental design: 100 dairy animals (32 cattle and 68 buffaloes) were randomly selected from the surveyed group of 200 dairy animals (76 cattle and 124 buffaloes) for the therapeutic trial and fed area specific mineral mixture @ 50g/animal/day for 3 months since the animals of Kandi area were deficit of micro and macro minerals as per the previous surveys conducted by other researchers (Singh, 2002 and Hundal et al., 2009) in this region. 32 animals (15 cattle and 17 buffaloes) were kept as control. Fodder (25) and soil (27) samples were also collected simultaneously from all the dairy units. These animals were grouped on the basis of age as group I (1-4 years), group II (5-7 years of age) and group III (>7 years). Tests and procedures: About 20 ml of blood sample was collected from each selected animal in heparinised mineral free glass vials. The samples were centrifuged @ 3000 rpm for 10-15 minutes immediately after collection and the o separated plasma samples were stored at-10 C temperature in deep freezer for subsequent mineral analysis. Also 2 ml of blood was collected in sterile plastic vials, containing disodium ethylene di-amine tetra acetic acid (Na EDTA) as anticoagulant, for estimation of 2 hematological parameters. Samples were collected on days 0 and 90 after feeding and one month after the cessation of mineral supplementation i.e. on day 120. Fodder and soil samples were collected following standard procedures and were packaged in plastic bags of suitable size for further processing. Hematology: Hemoglobin (Hb) was estimated by Drabkin's / cyanomethaemoglobin method and packed cell volume (PCV) by microhematocrit method. Hb and PCV were sufficient to know the anaemic state of the animals and less time consuming. Mineral analysis: For Ca and Mg estimation, samples were diluted with 0.1 per cent lanthanum chloride and
Haryana Veterinarian, 2020
REPORT-CUCURBIT GENETICS …, 1999
Methods. Plant material'Perlita'melon were grown singly in pots in a greenhouse using a... more Methods. Plant material'Perlita'melon were grown singly in pots in a greenhouse using a trellis system. Hermaphroditic flowers were pollinated and tagged at anthesis, with one to two fruits per plant were allowed to develop. Unripe fruit were harvested at 20, 30, and 40 ...
Haryana Veterinarian, 2018
The study was conducted to assess the mineral status of dairy animals of Kandi belt of Punjab. Bl... more The study was conducted to assess the mineral status of dairy animals of Kandi belt of Punjab. Blood samples were collected from 200 dairy animals (76 cattle and 124 buffaloes) randomly selected from 9 villages of 4 blocks of the 2 districts (Hoshiarpur and Nawanshahar). Fodder (25) and soil (27) samples were also collected simultaneously from all the dairy units. These animals were grouped on the basis of age as group I (1
Haryana Veterinarian, 2020
The present study was conducted to assess the effect of levamisole therapy on udder health of dai... more The present study was conducted to assess the effect of levamisole therapy on udder health of dairy cows. Cows in the levamisole group were administered Lemasol-75®@ 2.5mg/kg b.w., S/C, once weekly at day-35,-28,-21, and-14 prior to the expected calving date while cows in control group were not given any treatment. Results revealed lower incidence of subclinical mastitis in the levamisole group compared to the control group. The somatic cell count and standard plate count levels in the levamisole group were significantly (P<0.05) lower as compared to those in the control group. Daily milk yield in levamisole group was significantly (P<0.05) higher compared to the control group. Conclusively, levamisole administration during late dry period improves the early lactation udder health of dairy cows.
Ageing is the time related deterioration of the physiological functions, leading to the cell's in... more Ageing is the time related deterioration of the physiological functions, leading to the cell's inability to withstand external and internal stress. The causative factors for the time dependant deleterious process of ageing are yet not well defined and no single adequate molecular explanation for ageing is currently available (Singh et al., 2009). Increased oxidative stress and its definitive role in the age-related diseases is well documented fact in human beings (Pandey and Rizvi, 2010). One of the most popular explanations of how ageing occurs at the molecular level is the oxidative stress hypothesis. Free radicals are highly reactive molecules that are produced during normal metabolism in the body or after exposure to environmental pro-oxidants. Excess free radicals cause a dangerous chain reaction that can destroy nucleic acid, proteins, lipids and other cellular compounds (Haliwell and Gutteridge, 1999).
In this paper we analysed the signals of Gastrocnemius (GST) and Soleus (SOL) calf muscles from a... more In this paper we analysed the signals of Gastrocnemius (GST) and Soleus (SOL) calf muscles from a unilateral clubfoot patient (right leg normal and left clubfoot) during different stages (resting, flexion and extension) using EMG. The Raw and filtered [Root Mean Square (RMS) and Integrated EMG (IEMG)] signals acquired from the subject were quantified using (AcqKnowledge 3.9, BIOPAC MP-100 systems Inc.), for 120 seconds and the output was analysed using paired sample T-test. Significant differences were observed between the Root Mean Square (RMS) EMG and Integrated EMG (IEMG) muscle activity of normal and clubfoot leg during the three conditions for both GST and SOL muscles. With the mathematical modelling tools, the results were studied and was found that the calf muscle activity of affected leg varies from the normal leg during resting, flexion and extension stages. It was also studied and proved that relapses can occur even after surgical correction (Achilles tenotomy) on these patients with the accuracy of 80%.
The study was undertaken in Holstein Friesian crossbred dairy cows (n=16) in late gestation. The ... more The study was undertaken in Holstein Friesian crossbred dairy cows (n=16) in late gestation. The cows were divided into two equal groups of eight animals each. Group-1 was administered with four doses of Levamisole on 35, 28, 21 and 14 th day prior to parturition and Group-2 was control. Colostrum and blood samples were collected from both groups on calving day for colostrum and calving day as well as 28 th day in milking for blood and analysed. Evaluation revealed significantly (P<0.05) lower plasma Malondialdehyde (MDA) levels and higher Glutathione peroxidase (GPx), Glutathione S transferase (GST) and Superoxide dismutase enzyme (SOD) activity in Levamisole treated group as compared to control group. Serum and colostrum total Ig levels in Levamisole treated groups were significantly (P<0.05) higher as compared to control groups. The study concluded that Levamisole therapy during late dry period has positive effect on immunity in dairy cows.
The study evaluated the efficacy of multienzyme therapy as an adjunct or alternative to antibioti... more The study evaluated the efficacy of multienzyme therapy as an adjunct or alternative to antibiotic therapy in mastitic cows. Twenty one cows affected with clinical mastitis were selected and divided into three treatment groups: Group 1 (n=7) animals were treated with enrofloxacin @ 10 mg/kg b.w, I/M for 5 days. Group 2 (n=7) cows received an intramammary infusion consisting of a mixture of three enzymes viz. chymotrypsin (8 mg), trypsin (8 mg) and papain (4 mg) dissolved in 5 ml NS, at 24 hr. interval for 7 days. Group 3 (n=7) cows received a combination of both parental antibiotic (Enrocin) and intramammary infusion of multienzymes for 7 days at 24 hours interval. Milk samples were collected prior to the treatment (day 1) and on days 4, 7 and 14 post treatment and blood samples on day 1, 7 and 14. There was significant (P<0.05) decrease in the mean serum total protein (TP) and globulin levels in all the treatment groups post treatment as compared to the pretreatment levels while as the mean serum albumin levels and the A: G ratio showed significant (P<0.05) increase post treatment. There was a significant (P<0.05) decrease in the mean total sialic acid (TSA) levels on days 7 and 14 after treatment as compared to the pre-treatment levels in all the three groups. Mean somatic cell count (SCC), standard plate count (SPC) and electrical conductivity (EC) values decreased significantly on days 4, 7 and 14 as compared to that of the day 1. It was concluded that therapeutic intervention with parenteral enrofloxacin and intramammary infusion of proteolytic enzymes improved the udder health of dairy cows affected with clinicalmastitis.