Aguinaldo Garcez | Centro de Pesquisas em Odontologia Sao Leopoldo Mandic (original) (raw)

Papers by Aguinaldo Garcez

Oral Diseases

Hypogeusia and hyposmia (decreased gustatory and olfactory perception, respectively) have been re... more Hypogeusia and hyposmia (decreased gustatory and olfactory perception, respectively) have been reported in patients diagnosed with the novel corona virus disease (COVID19) (Russell et al., 2020). The most common symptoms of respiratory viruses include fever, dry cough, dyspnea, and fatigue (Lovato & de Filippis, 2020). Nonetheless, a significant number of patients affected by COVID19 appear to have loss of smell and taste, even before symptoms of fever or dry cough appear (Giacomelli et al., 2020 and Lechien et al., 2020). The first case of COVID19 in Brazil was reported on February 26, 2020. Meanwhile and on the same day, 131 patients were hospitalized for other respiratory syndromes (MSBrasil, 2020). To the best of our knowledge, no study has been presented reporting gustatory and olfactory disorders in patients affected by COVID19 in Brazil. Therefore, the aim of this study was to investigate the prevalence of hypogeusia and hyposmia comparing COVID19 and other respiratory syndromes (RS), through a crosssectional survey.

RGO - Revista Gaúcha de Odontologia

Objective The objective of this study was to 1) evaluate the reproducibility of natural head posi... more Objective The objective of this study was to 1) evaluate the reproducibility of natural head position (NHP) in different professional groups; 2) compare three cephalometric methods of estimating head position to the established natural head position and 3) investigate the applicability of a new method to estimated head position using a Centroid method. Methods The sample consisted of 105 individual cephalometric tracings and photographs. For the first evaluation, copies of the lateral photographs were reproduced for each patient and 3 professionals with different expertise (experienced orthodontists, orthodontic students and orthognatic surgeon) were instructed to position the photographs in NHP. Later, 3 different methods to obtain NHP were assessed: 1) the Frankfort Horizontal Plane (FRANK), 2) Sella-Nasion 7º Line (SN7) and 3) proposed Horizontal Reference Line based on Centroid. Angles formed by the evaluated planes/lines and True Vertical Line obtained were measured. Gender and...

RGO - Revista Gaúcha de Odontologia

The return to dental practice in pandemic times is a new challenge due to the generation and disp... more The return to dental practice in pandemic times is a new challenge due to the generation and dispersion of droplets and aerosols that may contain the SARS-CoV-2 virus, the etiological agent of covid-19. In the last months some droplet and aerosol containment strategies have been circulating on the internet, however, until now there is no evidence in the literature to prove the effectiveness of such barriers. Thus, using a microbial dispersion model with the fast handpiece, the aim of this preliminary study was to compare the dispersion caused by the the dental drill (DD) alone or in association with an individual biosafety barrier (IDBD / DD), which consisted of a layer of PVC film combined to a layer of polypropylene mounted on a frame. The dental drill was activated for one minute having had the water from the reservoir been replaced with a suspension of Lactobacillus casei Shirota. Petri dishes containing MRS agar were positioned at 50, 100 and 150 cm from the headboard of the de...

Photochemistry and Photobiology

Ventilator‐associated pneumonia (VAP) is an infection that arises after endotracheal intubation a... more Ventilator‐associated pneumonia (VAP) is an infection that arises after endotracheal intubation affecting patients under intensive care. The presence of the endotracheal tube (ETT) is a risk factor since it is colonized by multispecies biofilm. Antimicrobial photodynamic therapy (aPDT) could be a strategy to decontaminate ETTs. We verify if methylene blue (MB) associated with external illumination of the ETT could be an alternative to destroy biofilm. We performed an in vitro and ex vivo study. In vitro study was performed with P. aeruginosa biofilm grew over ETT for 7 days. After treatment, the surviving cells were cultured for 3 days and the biofilm was analyzed by crystal violet absorbance. Ex vivo study employed ETT obtained from extubated patients. aPDT was performed with MB (100 µm) and red LED (λ = 640±20 nm). We quantified the biofilm thickness and used scanning electron microscopy and fluorescence technique to verify morphological and functional changes after aPDT. Our results showed that bacteria remain susceptible to aPDT after sequential treatments. We also attested that aPDT can reduce biofilm thickness, disrupt biofilm attachment from ETT surface and kill microbial cells. These data suggest that aPDT should be investigated to decrease VAP incidence via ETT decontamination.

The Journal of Contemporary Dental Practice

Aim: This case report aimed to present an orthodontic mechanic alternative for space opening in a... more Aim: This case report aimed to present an orthodontic mechanic alternative for space opening in a patient missing maxillary lateral incisors, using miniplates as anchorage, associated with self-ligating brackets. Background: Dental agenesis affects the population causing esthetic and functional damages. The prevalence of missing maxillary lateral incisors should be considered for its significant rate and negative impact on smile esthetics. Case report: This treatment was chosen based on the presence of balanced facial pattern, large canine anatomy, the need to improve dental occlusion to prevent further wear, and patient's esthetic complaint. To obtain the results, upper third molars were extracted, and two miniplates were installed to distalize the upper arch with no need for patient compliance or auxiliary devices. Self-ligating brackets were used to reduce friction on posterior teeth, thus facilitating movement with light force application. At the end of 19 months, the patient presented with class I good overbite and overjet, and adequate space for implant and prosthetics; also, good facial esthetic was maintained. Conclusion: When indicated, space opening may provide excellent esthetics and functional results, and even more predictable results when skeletal anchorage miniplates are used to distalize all posterior teeth.

Journal of Biophotonics

This study investigated the effects of photobiomodulation (PBM) on upper molar intrusion movement... more This study investigated the effects of photobiomodulation (PBM) on upper molar intrusion movement, regarding acceleration of orthodontic movement and its molecular effects. The sample consisted of 30 patients with indication of tooth intrusion for oral rehabilitation. Teeth were divided into three different groups: G1 (n=10) pre-molars without force or laser application (control); G2 (n=10) upper molar intrusion; and G3 (n=10) upper molar intrusion and PBM. On PBM treated molars, the teeth were irradiated with a low-power diode laser (808nm, 100mW), receiving 1J per point, density of 25 J/cm2 , with application of 10 s per point, 10 points (5 per vestibular and 5 per palatal region). Orthodontic force of intrusion applied every 30 days and PBM was performed immediately, 3 and 7 days after force application for 3 months. Gingival crevicular fluid was collected at the same time periods as the laser applications and interleukins 1-β, 6 and 8 were evaluated by ELISA. Clinical measures were performed monthly to verify the amount of intrusion. The levels of IL-6, IL-8 and IL-1β increased under orthodontic force (G2 and G3) when compared to control group (G1), however the citokynes levels were significantly higher after PBM (G3). The mean intrusion velocity was 0.26mm/month in the irradiated group (G3), average duration of 8 months versus 0.17mm/month for the non-irradiated group (G2), average duration of 12 months. This study suggests that PBM accelerates tooth movement during molar intrusion, due to modulation of IL-6, IL-8 and IL-1β during bone remodeling. This article is protected by copyright. All rights reserved.

European Endodontic Journal

Antimicrobial photodynamic therapy (aPDT) is a controversial approach for endodontic disinfection... more Antimicrobial photodynamic therapy (aPDT) is a controversial approach for endodontic disinfection. The objective of this study was to test the photosensitiser (PS) concentration and assess the optical shielding phenomenon, the use of hydrogen peroxide (H 2 O 2) and minimal energy irradiation to optimise endodontic aPDT for suggesting a protocol for clinical use. Methods: Different parameters for aPDT were tested. Aqueous solutions of methylene blue (MB) at 50, 100, 150 and 300 µM were tested in vitro for optical shield and reactive oxygen species (ROS) production by the reduction of N,N-dimethyl-4-notrosoaniline (RNO) at 440 nm absorbance when irradiated using a diode laser (660 nm). Ten single-rooted teeth were inoculated with bioluminescent bacteria Pseudomonas aeruginosa for 72 hours to form biofilms. Bioluminescence imaging was used to serially evaluate the minimum energy necessary during endodontic aPDT using MB and a diode laser coupled to an optical fibre for intracanal microbial reduction. In addition, teeth (n=21) infected with Enterococcus faecalis were treated with sequential combinations of endodontic aPDT and H 2 O 2 and the colony-forming unit (CFU) was determined. Results: ROS production was inversely proportional to the MB concentration in the solution due to quenching of MB. Optical shielding limited light penetration at high MB concentrations. The use of H 2 O 2 before aPDT achieved higher disinfection compared to conventional aPDT or when MB was irradiated in an H 2 O 2 solution. Energy irradiation of 9.6 J achieved a significant reduction and further light delivery did not produce further reduction. Conclusion: PS concentration of about 50 µM, biofilm pre-treatment with H 2 O 2 for 1 min and energy irradiation around 10 J appear to be an effective protocol for endodontic aPDT.

Photochemistry and Photobiology

Lasers in medical science, 2018

The aim of this study was to compare the rate of tooth displacement, quantity of root resorption,... more The aim of this study was to compare the rate of tooth displacement, quantity of root resorption, and alveolar bone changes in five groups: corticopuncture (CP), low-level laser therapy (LLLT), CP combined with LLLT (CP + LLLT), control (C), and negative control (NC). A total of 60 half-maxilla from 30 male Wistar rats (10 weeks old) were divided randomly into five groups: three (CP, LLLT, and CP + LLLT) test groups with different stimulation for accelerated-tooth-movement (ATM), one control (C) group, and one negative control (NC) group with no tooth movement. Nickel-titanium coil springs with 50 g of force were tied from the upper left and right first molars to micro-implants placed behind the maxillary incisors. For the CP and CP + LLLT groups, two perforations in the palate and one mesially to the molars were performed. For the LLLT and CP + LLLT groups, GaAlAs diode laser was applied every other day for 14 days (810 nm, 100 mW, 15 s). The tooth displacements were measured direc...

Photomedicine and Laser Surgery, 2016

The primary stability of a mini-implant is crucial to treatment sequence since most orthodontic m... more The primary stability of a mini-implant is crucial to treatment sequence since most orthodontic mini-implant failures occur at an early stage. Irritation or inflammation of peri-implant tissues has been related to decreasing mini-implant success. This study evaluates the effect of low-level laser therapy on initial inflammation after orthodontic mini-implants installation. Ten volunteers received two mini-implants (1.3 mm diameter, 7 mm length). One mini-implant was inserted on each side of the maxilla following manufacturer recommendation. On the right side, low-level laser therapy (LLLT) was applied (diode laser 660 nm, 40 mW, 1 min, 2.4 J of total energy). Peri-implant crevicular fluid (PGF) was obtained after 24 h (T1), 48 h (T2), and 72 h (T3) to identify levels of interleukin (IL)-6 and IL-8 around mini-implants and around upper first premolars. An increase in interleukin levels was observed for both groups, compared to upper first premolar. PGF around nonirradiated mini-implants showed higher levels of IL-8. Levels of IL-6 24 h after mini-implant insertion were higher for laser group. LLLT modulates the initial inflammation after the insertion of mini-implant, possibly increasing the mini-implant success prognostic and decreasing patient discomfort.

Journal of Biophotonics, 2016

This study evaluated the biological effects of low-level laser therapy (LLLT) on bone remodeling,... more This study evaluated the biological effects of low-level laser therapy (LLLT) on bone remodeling, tooth displacement and root resorption, occurred during the orthodontic tooth movement. Upper first molars of a total of sixtyeight male rats were subjected to orthodontic tooth movement and euthanized on days 3, 6, 9, 14 and 21 days and divided as negative control, control and LLLT group. Tooth displacement and histomorphometric analysis were performed in all animals; scanning electron microscopy analysis was done on days 3, 6 and 9, as well as the immunohistochemistry analysis of RANKL/OPG and TRAP markers. Volumetric changes in alveolar bone were analyzed using MicroCT images on days 14 and 21. LLLT influenced bone resorption by increasing the number of TRAP-positive osteoclasts and the RANKL expression at the compression side. This resulted in less alveolar bone and hyalinization areas on days 6, 9 and 14. LLLT also induced less bone volume and density, facilitating significant acceleration of tooth movement and potential reduction in root resorption besides stimulating bone formation at the tension side by enhancing OPG expression, increasing trabecular thickness and bone volume on day 21. Taken together, our results indicate that LLLT can stimulate bone remodeling reducing root resorption in a rat model. LLLT improves tooth movement via bone formation and bone resorption in a rat model.

Dental Press Journal of Orthodontics, 2016

The midpalatal suture has bone margins with thick connective tissue interposed between them, and ... more The midpalatal suture has bone margins with thick connective tissue interposed between them, and it does not represent the fusion of maxillary palatal processes only, but also the fusion of palatal processes of the jaws and horizontal osseous laminae of palatal bones. Changing it implies affecting neighboring areas. It has got three segments that should be considered by all clinical analyses, whether therapeutic or experimental: the anterior segment (before the incisive foramen, or intermaxillary segment), the middle segment (from the incisive foramen to the suture transversal to the palatal bone ) and the posterior segment (after the suture transversal to the palatal bone ). Rapid palatal expansion might be recommended for patients at the final pubertal growth stage, in addition to adult patients with maxillary constriction. It represents a treatment solution that can potentially avoid surgical intervention. When performed in association with rapid palatal expanders, it might enhan...

International Journal of Orthodontics, 2013

The aim of this case report was to demonstrate the efficient use of miniscrew as anchorage in a 1... more The aim of this case report was to demonstrate the efficient use of miniscrew as anchorage in a 12-year-old male patient with skeletal and dental Class II and severe biprotrusion and lips procumbency. It was proposed a compensating orthodontic treatment with 4 premolar extraction and maximum anchorage. Since the patient abandoned treatment after one premolar extraction, loss of anchorage occurred and treatment plan was modified to maximize lower retraction with miniscrew anchorage and distalization of the upper arch using two methods: Class II elastics from lower miniscrews to the sliding jig and modified transpalatal arch using palatal miniscrew. The results showed a significant improvement of his facial appearance, lip protrusion and also dental esthetics and function. It was concluded that miniscrews can provide absolute anchorage for maximum anterior retraction in order to correct dentoalveolar biprotrusion in the presence of limited thickness alveolar bone.

Revista Clínica de Ortodontia Dental Press, 2016

Proceedings of Spie the International Society For Optical Engineering, Apr 1, 2010

Photodynamic antimicrobial therapy (PAT) may become a useful clinical tool to treat microbial inf... more Photodynamic antimicrobial therapy (PAT) may become a useful clinical tool to treat microbial infections, overcoming microbial resistance that is a major problem nowadays. The aim of our work was to verify the damage caused by photosensitization over a Escherichia col) via atomic force microscopy (AFM), looking for structural changes that might occur in cells after PAT. Cells culture were grown

... Aguinaldo S Garcez*3, Silvia С Nunez3, Michael R Hamblinb and Martha S Ribeiro3. ... 50,1402-... more ... Aguinaldo S Garcez*3, Silvia С Nunez3, Michael R Hamblinb and Martha S Ribeiro3. ... 50,1402-10, 2006 29 - Gutknecht N, van Gogswaardt D, Conrads G, Apel С, Schubert С, Lampert F. Diode laser radiation and its bactericidal effect in root canal wall dentin. ...

Journal of the Brazilian Chemical Society, 2015

Oral Diseases

Hypogeusia and hyposmia (decreased gustatory and olfactory perception, respectively) have been re... more Hypogeusia and hyposmia (decreased gustatory and olfactory perception, respectively) have been reported in patients diagnosed with the novel corona virus disease (COVID19) (Russell et al., 2020). The most common symptoms of respiratory viruses include fever, dry cough, dyspnea, and fatigue (Lovato & de Filippis, 2020). Nonetheless, a significant number of patients affected by COVID19 appear to have loss of smell and taste, even before symptoms of fever or dry cough appear (Giacomelli et al., 2020 and Lechien et al., 2020). The first case of COVID19 in Brazil was reported on February 26, 2020. Meanwhile and on the same day, 131 patients were hospitalized for other respiratory syndromes (MSBrasil, 2020). To the best of our knowledge, no study has been presented reporting gustatory and olfactory disorders in patients affected by COVID19 in Brazil. Therefore, the aim of this study was to investigate the prevalence of hypogeusia and hyposmia comparing COVID19 and other respiratory syndromes (RS), through a crosssectional survey.

RGO - Revista Gaúcha de Odontologia

Objective The objective of this study was to 1) evaluate the reproducibility of natural head posi... more Objective The objective of this study was to 1) evaluate the reproducibility of natural head position (NHP) in different professional groups; 2) compare three cephalometric methods of estimating head position to the established natural head position and 3) investigate the applicability of a new method to estimated head position using a Centroid method. Methods The sample consisted of 105 individual cephalometric tracings and photographs. For the first evaluation, copies of the lateral photographs were reproduced for each patient and 3 professionals with different expertise (experienced orthodontists, orthodontic students and orthognatic surgeon) were instructed to position the photographs in NHP. Later, 3 different methods to obtain NHP were assessed: 1) the Frankfort Horizontal Plane (FRANK), 2) Sella-Nasion 7º Line (SN7) and 3) proposed Horizontal Reference Line based on Centroid. Angles formed by the evaluated planes/lines and True Vertical Line obtained were measured. Gender and...

RGO - Revista Gaúcha de Odontologia

The return to dental practice in pandemic times is a new challenge due to the generation and disp... more The return to dental practice in pandemic times is a new challenge due to the generation and dispersion of droplets and aerosols that may contain the SARS-CoV-2 virus, the etiological agent of covid-19. In the last months some droplet and aerosol containment strategies have been circulating on the internet, however, until now there is no evidence in the literature to prove the effectiveness of such barriers. Thus, using a microbial dispersion model with the fast handpiece, the aim of this preliminary study was to compare the dispersion caused by the the dental drill (DD) alone or in association with an individual biosafety barrier (IDBD / DD), which consisted of a layer of PVC film combined to a layer of polypropylene mounted on a frame. The dental drill was activated for one minute having had the water from the reservoir been replaced with a suspension of Lactobacillus casei Shirota. Petri dishes containing MRS agar were positioned at 50, 100 and 150 cm from the headboard of the de...

Photochemistry and Photobiology

Ventilator‐associated pneumonia (VAP) is an infection that arises after endotracheal intubation a... more Ventilator‐associated pneumonia (VAP) is an infection that arises after endotracheal intubation affecting patients under intensive care. The presence of the endotracheal tube (ETT) is a risk factor since it is colonized by multispecies biofilm. Antimicrobial photodynamic therapy (aPDT) could be a strategy to decontaminate ETTs. We verify if methylene blue (MB) associated with external illumination of the ETT could be an alternative to destroy biofilm. We performed an in vitro and ex vivo study. In vitro study was performed with P. aeruginosa biofilm grew over ETT for 7 days. After treatment, the surviving cells were cultured for 3 days and the biofilm was analyzed by crystal violet absorbance. Ex vivo study employed ETT obtained from extubated patients. aPDT was performed with MB (100 µm) and red LED (λ = 640±20 nm). We quantified the biofilm thickness and used scanning electron microscopy and fluorescence technique to verify morphological and functional changes after aPDT. Our results showed that bacteria remain susceptible to aPDT after sequential treatments. We also attested that aPDT can reduce biofilm thickness, disrupt biofilm attachment from ETT surface and kill microbial cells. These data suggest that aPDT should be investigated to decrease VAP incidence via ETT decontamination.

The Journal of Contemporary Dental Practice

Aim: This case report aimed to present an orthodontic mechanic alternative for space opening in a... more Aim: This case report aimed to present an orthodontic mechanic alternative for space opening in a patient missing maxillary lateral incisors, using miniplates as anchorage, associated with self-ligating brackets. Background: Dental agenesis affects the population causing esthetic and functional damages. The prevalence of missing maxillary lateral incisors should be considered for its significant rate and negative impact on smile esthetics. Case report: This treatment was chosen based on the presence of balanced facial pattern, large canine anatomy, the need to improve dental occlusion to prevent further wear, and patient's esthetic complaint. To obtain the results, upper third molars were extracted, and two miniplates were installed to distalize the upper arch with no need for patient compliance or auxiliary devices. Self-ligating brackets were used to reduce friction on posterior teeth, thus facilitating movement with light force application. At the end of 19 months, the patient presented with class I good overbite and overjet, and adequate space for implant and prosthetics; also, good facial esthetic was maintained. Conclusion: When indicated, space opening may provide excellent esthetics and functional results, and even more predictable results when skeletal anchorage miniplates are used to distalize all posterior teeth.

Journal of Biophotonics

This study investigated the effects of photobiomodulation (PBM) on upper molar intrusion movement... more This study investigated the effects of photobiomodulation (PBM) on upper molar intrusion movement, regarding acceleration of orthodontic movement and its molecular effects. The sample consisted of 30 patients with indication of tooth intrusion for oral rehabilitation. Teeth were divided into three different groups: G1 (n=10) pre-molars without force or laser application (control); G2 (n=10) upper molar intrusion; and G3 (n=10) upper molar intrusion and PBM. On PBM treated molars, the teeth were irradiated with a low-power diode laser (808nm, 100mW), receiving 1J per point, density of 25 J/cm2 , with application of 10 s per point, 10 points (5 per vestibular and 5 per palatal region). Orthodontic force of intrusion applied every 30 days and PBM was performed immediately, 3 and 7 days after force application for 3 months. Gingival crevicular fluid was collected at the same time periods as the laser applications and interleukins 1-β, 6 and 8 were evaluated by ELISA. Clinical measures were performed monthly to verify the amount of intrusion. The levels of IL-6, IL-8 and IL-1β increased under orthodontic force (G2 and G3) when compared to control group (G1), however the citokynes levels were significantly higher after PBM (G3). The mean intrusion velocity was 0.26mm/month in the irradiated group (G3), average duration of 8 months versus 0.17mm/month for the non-irradiated group (G2), average duration of 12 months. This study suggests that PBM accelerates tooth movement during molar intrusion, due to modulation of IL-6, IL-8 and IL-1β during bone remodeling. This article is protected by copyright. All rights reserved.

European Endodontic Journal

Antimicrobial photodynamic therapy (aPDT) is a controversial approach for endodontic disinfection... more Antimicrobial photodynamic therapy (aPDT) is a controversial approach for endodontic disinfection. The objective of this study was to test the photosensitiser (PS) concentration and assess the optical shielding phenomenon, the use of hydrogen peroxide (H 2 O 2) and minimal energy irradiation to optimise endodontic aPDT for suggesting a protocol for clinical use. Methods: Different parameters for aPDT were tested. Aqueous solutions of methylene blue (MB) at 50, 100, 150 and 300 µM were tested in vitro for optical shield and reactive oxygen species (ROS) production by the reduction of N,N-dimethyl-4-notrosoaniline (RNO) at 440 nm absorbance when irradiated using a diode laser (660 nm). Ten single-rooted teeth were inoculated with bioluminescent bacteria Pseudomonas aeruginosa for 72 hours to form biofilms. Bioluminescence imaging was used to serially evaluate the minimum energy necessary during endodontic aPDT using MB and a diode laser coupled to an optical fibre for intracanal microbial reduction. In addition, teeth (n=21) infected with Enterococcus faecalis were treated with sequential combinations of endodontic aPDT and H 2 O 2 and the colony-forming unit (CFU) was determined. Results: ROS production was inversely proportional to the MB concentration in the solution due to quenching of MB. Optical shielding limited light penetration at high MB concentrations. The use of H 2 O 2 before aPDT achieved higher disinfection compared to conventional aPDT or when MB was irradiated in an H 2 O 2 solution. Energy irradiation of 9.6 J achieved a significant reduction and further light delivery did not produce further reduction. Conclusion: PS concentration of about 50 µM, biofilm pre-treatment with H 2 O 2 for 1 min and energy irradiation around 10 J appear to be an effective protocol for endodontic aPDT.

Photochemistry and Photobiology

Lasers in medical science, 2018

The aim of this study was to compare the rate of tooth displacement, quantity of root resorption,... more The aim of this study was to compare the rate of tooth displacement, quantity of root resorption, and alveolar bone changes in five groups: corticopuncture (CP), low-level laser therapy (LLLT), CP combined with LLLT (CP + LLLT), control (C), and negative control (NC). A total of 60 half-maxilla from 30 male Wistar rats (10 weeks old) were divided randomly into five groups: three (CP, LLLT, and CP + LLLT) test groups with different stimulation for accelerated-tooth-movement (ATM), one control (C) group, and one negative control (NC) group with no tooth movement. Nickel-titanium coil springs with 50 g of force were tied from the upper left and right first molars to micro-implants placed behind the maxillary incisors. For the CP and CP + LLLT groups, two perforations in the palate and one mesially to the molars were performed. For the LLLT and CP + LLLT groups, GaAlAs diode laser was applied every other day for 14 days (810 nm, 100 mW, 15 s). The tooth displacements were measured direc...

Photomedicine and Laser Surgery, 2016

The primary stability of a mini-implant is crucial to treatment sequence since most orthodontic m... more The primary stability of a mini-implant is crucial to treatment sequence since most orthodontic mini-implant failures occur at an early stage. Irritation or inflammation of peri-implant tissues has been related to decreasing mini-implant success. This study evaluates the effect of low-level laser therapy on initial inflammation after orthodontic mini-implants installation. Ten volunteers received two mini-implants (1.3 mm diameter, 7 mm length). One mini-implant was inserted on each side of the maxilla following manufacturer recommendation. On the right side, low-level laser therapy (LLLT) was applied (diode laser 660 nm, 40 mW, 1 min, 2.4 J of total energy). Peri-implant crevicular fluid (PGF) was obtained after 24 h (T1), 48 h (T2), and 72 h (T3) to identify levels of interleukin (IL)-6 and IL-8 around mini-implants and around upper first premolars. An increase in interleukin levels was observed for both groups, compared to upper first premolar. PGF around nonirradiated mini-implants showed higher levels of IL-8. Levels of IL-6 24 h after mini-implant insertion were higher for laser group. LLLT modulates the initial inflammation after the insertion of mini-implant, possibly increasing the mini-implant success prognostic and decreasing patient discomfort.

Journal of Biophotonics, 2016

This study evaluated the biological effects of low-level laser therapy (LLLT) on bone remodeling,... more This study evaluated the biological effects of low-level laser therapy (LLLT) on bone remodeling, tooth displacement and root resorption, occurred during the orthodontic tooth movement. Upper first molars of a total of sixtyeight male rats were subjected to orthodontic tooth movement and euthanized on days 3, 6, 9, 14 and 21 days and divided as negative control, control and LLLT group. Tooth displacement and histomorphometric analysis were performed in all animals; scanning electron microscopy analysis was done on days 3, 6 and 9, as well as the immunohistochemistry analysis of RANKL/OPG and TRAP markers. Volumetric changes in alveolar bone were analyzed using MicroCT images on days 14 and 21. LLLT influenced bone resorption by increasing the number of TRAP-positive osteoclasts and the RANKL expression at the compression side. This resulted in less alveolar bone and hyalinization areas on days 6, 9 and 14. LLLT also induced less bone volume and density, facilitating significant acceleration of tooth movement and potential reduction in root resorption besides stimulating bone formation at the tension side by enhancing OPG expression, increasing trabecular thickness and bone volume on day 21. Taken together, our results indicate that LLLT can stimulate bone remodeling reducing root resorption in a rat model. LLLT improves tooth movement via bone formation and bone resorption in a rat model.

Dental Press Journal of Orthodontics, 2016

The midpalatal suture has bone margins with thick connective tissue interposed between them, and ... more The midpalatal suture has bone margins with thick connective tissue interposed between them, and it does not represent the fusion of maxillary palatal processes only, but also the fusion of palatal processes of the jaws and horizontal osseous laminae of palatal bones. Changing it implies affecting neighboring areas. It has got three segments that should be considered by all clinical analyses, whether therapeutic or experimental: the anterior segment (before the incisive foramen, or intermaxillary segment), the middle segment (from the incisive foramen to the suture transversal to the palatal bone ) and the posterior segment (after the suture transversal to the palatal bone ). Rapid palatal expansion might be recommended for patients at the final pubertal growth stage, in addition to adult patients with maxillary constriction. It represents a treatment solution that can potentially avoid surgical intervention. When performed in association with rapid palatal expanders, it might enhan...

International Journal of Orthodontics, 2013

The aim of this case report was to demonstrate the efficient use of miniscrew as anchorage in a 1... more The aim of this case report was to demonstrate the efficient use of miniscrew as anchorage in a 12-year-old male patient with skeletal and dental Class II and severe biprotrusion and lips procumbency. It was proposed a compensating orthodontic treatment with 4 premolar extraction and maximum anchorage. Since the patient abandoned treatment after one premolar extraction, loss of anchorage occurred and treatment plan was modified to maximize lower retraction with miniscrew anchorage and distalization of the upper arch using two methods: Class II elastics from lower miniscrews to the sliding jig and modified transpalatal arch using palatal miniscrew. The results showed a significant improvement of his facial appearance, lip protrusion and also dental esthetics and function. It was concluded that miniscrews can provide absolute anchorage for maximum anterior retraction in order to correct dentoalveolar biprotrusion in the presence of limited thickness alveolar bone.

Revista Clínica de Ortodontia Dental Press, 2016

Proceedings of Spie the International Society For Optical Engineering, Apr 1, 2010

Photodynamic antimicrobial therapy (PAT) may become a useful clinical tool to treat microbial inf... more Photodynamic antimicrobial therapy (PAT) may become a useful clinical tool to treat microbial infections, overcoming microbial resistance that is a major problem nowadays. The aim of our work was to verify the damage caused by photosensitization over a Escherichia col) via atomic force microscopy (AFM), looking for structural changes that might occur in cells after PAT. Cells culture were grown

... Aguinaldo S Garcez*3, Silvia С Nunez3, Michael R Hamblinb and Martha S Ribeiro3. ... 50,1402-... more ... Aguinaldo S Garcez*3, Silvia С Nunez3, Michael R Hamblinb and Martha S Ribeiro3. ... 50,1402-10, 2006 29 - Gutknecht N, van Gogswaardt D, Conrads G, Apel С, Schubert С, Lampert F. Diode laser radiation and its bactericidal effect in root canal wall dentin. ...

Journal of the Brazilian Chemical Society, 2015