Olga Shamova | Saint-Petersburg State University (original) (raw)

Papers by Olga Shamova

We identified two novel ␤-defensin precursors, preproGBD-1 and preproGBD-2, in the tissues of a g... more We identified two novel ␤-defensin precursors, preproGBD-1 and preproGBD-2, in the tissues of a goat. Although the precursors were identical in 96.8% of their bases and 88.2% (60 of 68) of their amino acids, preproGBD-1 was expressed principally in the tongue and respiratory tract, whereas preproGBD-2 expression predominated throughout the intestine. These findings exemplify the phenomenon of tissue-specific expression in a family of host defense peptides that arose before the avian and mammalian lineages diverged.

Int J Pept Res Ther, 2009

We isolated a new proline-rich peptide, ChBac3.4, from leukocytes of the goat (Capra hirca) and d... more We isolated a new proline-rich peptide, ChBac3.4, from leukocytes of the goat (Capra hirca) and determined its amino acid sequence by Edman degradation and mass spectrometry. ChBac3.4 (RFRLPFRRPPIRIHP PPFYPPFRRFL-NH2) had over 50% sequence identity to the Bac5 peptides found in the leukocytes of goats, sheep and cattle.

We purified three proline-rich antimicrobial peptides from elastase-treated extracts of sheep and... more We purified three proline-rich antimicrobial peptides from elastase-treated extracts of sheep and goat leukocytes and subjected two of them, OaBac5a and ChBac5, to detailed analysis. OaBac5a and ChBac5 were homologous to each other and to bovine Bac5. Both exhibited potent, broad-spectrum antimicrobial activity under low-concentration salt conditions. While the peptides remained active against Escherichia coli, Pseudo- monas aeruginosa, Bacillus subtilis,

PROTEOMICS, 2009

A novel peptide with antimicrobial activity was isolated from leukocytes of the European pond tur... more A novel peptide with antimicrobial activity was isolated from leukocytes of the European pond turtle Emys orbicularis and purified to homogeneity by preparative gel electrophoresis followed by reversed phase chromatography. It was highly active in vitro against Escherichia coli, Listeria monocytogenes, methicillin-resistant Staphylococcus aureus, and Candida albicans. The isolated peptide was sequenced de novo by tandem mass spectrometry using both collision-induced and electron-transfer dissociation in combination with different chemical derivatization techniques. The 40-residue peptide, called TBD-1 (turtle b-defensin 1), represents the first defensin isolated from reptilian leukocytes. It contains three disulfide bonds and shows high structural similarities to b-defensins isolated from birds and mammals. Abbreviations: AMA, antimicrobial activity; AMP, antimicrobial peptide; AU, acid urea; ETD, electron-transfer dissociation; HFBA, heptafluorobutyric acid; MIC, minimal inhibitory concentration; MRSA, methicillin-resistant Staphylococcus aureus; Qq, double quadrupole; SACA, 2-sulfobenzoic acid cyclic anhydride; SPITC, 4-sulfophenyl isothiocyanate sodium salt monohydrate; TBD-1, turtle b-defensin 1

Journal of Antimicrobial Chemotherapy, 2007

Objectives: Cationic antimicrobial peptides (AMPs) are indispensable components of innate immune ... more Objectives: Cationic antimicrobial peptides (AMPs) are indispensable components of innate immune systems and promising candidates for novel anti-infective strategies. We rationally designed a series of peptides based on a template derived from known a-helical AMPs, which were then analysed regarding efficacy against clinical isolates and antibiotic mechanisms.

FEBS Letters, 1993

The primary structure of three major cationic peptides from porcine neutrophils has been determin... more The primary structure of three major cationic peptides from porcine neutrophils has been determined. The sequencing was made by the combined use of electrospray ionization mass spectrometry and Edman degradation. The determined sequences unambiguously show that these peptides can not be considered as defensins.

Infection and Immunity, 2010

Human ␤-defensin 3 (hBD3) is a highly charged (؉11) cationic host defense peptide, produced by ep... more Human ␤-defensin 3 (hBD3) is a highly charged (؉11) cationic host defense peptide, produced by epithelial cells and neutrophils. hBD3 retains antimicrobial activity against a broad range of pathogens, including multiresistant Staphylococcus aureus, even under high-salt conditions. Whereas antimicrobial host defense peptides are assumed to act by permeabilizing cell membranes, the transcriptional response pattern of hBD3-treated staphylococcal cells resembled that of vancomycin-treated cells (V. Sass, U. Pag, A. Tossi, G. Bierbaum, and H. G. Sahl, Int. J. Med. Microbiol. 298:619-633, 2008) and suggested that inhibition of cell wall biosynthesis is a major component of the killing process. hBD3-treated cells, inspected by transmission electron microscopy, showed localized protrusions of cytoplasmic contents, and analysis of the intracellular pool of nucleotide-activated cell wall precursors demonstrated accumulation of the final soluble precursor,

We identified two novel ␤-defensin precursors, preproGBD-1 and preproGBD-2, in the tissues of a g... more We identified two novel ␤-defensin precursors, preproGBD-1 and preproGBD-2, in the tissues of a goat. Although the precursors were identical in 96.8% of their bases and 88.2% (60 of 68) of their amino acids, preproGBD-1 was expressed principally in the tongue and respiratory tract, whereas preproGBD-2 expression predominated throughout the intestine. These findings exemplify the phenomenon of tissue-specific expression in a family of host defense peptides that arose before the avian and mammalian lineages diverged.

Int J Pept Res Ther, 2009

We isolated a new proline-rich peptide, ChBac3.4, from leukocytes of the goat (Capra hirca) and d... more We isolated a new proline-rich peptide, ChBac3.4, from leukocytes of the goat (Capra hirca) and determined its amino acid sequence by Edman degradation and mass spectrometry. ChBac3.4 (RFRLPFRRPPIRIHP PPFYPPFRRFL-NH2) had over 50% sequence identity to the Bac5 peptides found in the leukocytes of goats, sheep and cattle.

We purified three proline-rich antimicrobial peptides from elastase-treated extracts of sheep and... more We purified three proline-rich antimicrobial peptides from elastase-treated extracts of sheep and goat leukocytes and subjected two of them, OaBac5a and ChBac5, to detailed analysis. OaBac5a and ChBac5 were homologous to each other and to bovine Bac5. Both exhibited potent, broad-spectrum antimicrobial activity under low-concentration salt conditions. While the peptides remained active against Escherichia coli, Pseudo- monas aeruginosa, Bacillus subtilis,

PROTEOMICS, 2009

A novel peptide with antimicrobial activity was isolated from leukocytes of the European pond tur... more A novel peptide with antimicrobial activity was isolated from leukocytes of the European pond turtle Emys orbicularis and purified to homogeneity by preparative gel electrophoresis followed by reversed phase chromatography. It was highly active in vitro against Escherichia coli, Listeria monocytogenes, methicillin-resistant Staphylococcus aureus, and Candida albicans. The isolated peptide was sequenced de novo by tandem mass spectrometry using both collision-induced and electron-transfer dissociation in combination with different chemical derivatization techniques. The 40-residue peptide, called TBD-1 (turtle b-defensin 1), represents the first defensin isolated from reptilian leukocytes. It contains three disulfide bonds and shows high structural similarities to b-defensins isolated from birds and mammals. Abbreviations: AMA, antimicrobial activity; AMP, antimicrobial peptide; AU, acid urea; ETD, electron-transfer dissociation; HFBA, heptafluorobutyric acid; MIC, minimal inhibitory concentration; MRSA, methicillin-resistant Staphylococcus aureus; Qq, double quadrupole; SACA, 2-sulfobenzoic acid cyclic anhydride; SPITC, 4-sulfophenyl isothiocyanate sodium salt monohydrate; TBD-1, turtle b-defensin 1

Journal of Antimicrobial Chemotherapy, 2007

Objectives: Cationic antimicrobial peptides (AMPs) are indispensable components of innate immune ... more Objectives: Cationic antimicrobial peptides (AMPs) are indispensable components of innate immune systems and promising candidates for novel anti-infective strategies. We rationally designed a series of peptides based on a template derived from known a-helical AMPs, which were then analysed regarding efficacy against clinical isolates and antibiotic mechanisms.

FEBS Letters, 1993

The primary structure of three major cationic peptides from porcine neutrophils has been determin... more The primary structure of three major cationic peptides from porcine neutrophils has been determined. The sequencing was made by the combined use of electrospray ionization mass spectrometry and Edman degradation. The determined sequences unambiguously show that these peptides can not be considered as defensins.

Infection and Immunity, 2010

Human ␤-defensin 3 (hBD3) is a highly charged (؉11) cationic host defense peptide, produced by ep... more Human ␤-defensin 3 (hBD3) is a highly charged (؉11) cationic host defense peptide, produced by epithelial cells and neutrophils. hBD3 retains antimicrobial activity against a broad range of pathogens, including multiresistant Staphylococcus aureus, even under high-salt conditions. Whereas antimicrobial host defense peptides are assumed to act by permeabilizing cell membranes, the transcriptional response pattern of hBD3-treated staphylococcal cells resembled that of vancomycin-treated cells (V. Sass, U. Pag, A. Tossi, G. Bierbaum, and H. G. Sahl, Int. J. Med. Microbiol. 298:619-633, 2008) and suggested that inhibition of cell wall biosynthesis is a major component of the killing process. hBD3-treated cells, inspected by transmission electron microscopy, showed localized protrusions of cytoplasmic contents, and analysis of the intracellular pool of nucleotide-activated cell wall precursors demonstrated accumulation of the final soluble precursor,