Vahid Zarrin nia | Islamic Azad University, Science and Research Branch (original) (raw)

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Papers by Vahid Zarrin nia

Rice blast, causes by Magnaporthe grisea (sensu lato), is one of the most important and destructi... more Rice blast, causes by Magnaporthe grisea (sensu lato), is one of the most important and destructive disease of rice in Iran.
Genetic and population structure of M. grisea s.l was examined using data derived from 7 primer pairs of polymorphic minisatellite locus by Nei’s test for population differentiation, G
, and analysis of molecular variation (AMOVA). In this research 92 isolates of the fungus were collected from diverse geographical locations and hosts such as Digitaria singuinalis, Setaria italica, Echinochloa sp.,
ST
Eragrostis curvula, Festuca arundinacea, Stenotaphrum secundatum, Panicum miliaceum, Triticum aestivum, Lolium perenne from Japan, USA and most important rice growing regions in north of Iran.
The results revealed that M. grisea s.l populations were composed of eight lineages designated as A to H. Among the lineages, lineage C with 25 (22.7%) isolates were considered as dominant lineage. All isolates collected from Digitaria sanguinalis established a discrete lineage. Also isolates from rice were closely related to those from Setaria italica, Echinochloa sp., Eragrostis curvula, Festuca arundinacea, Stenotaphrum secundatum, Panicum miliaceum, Triticum aestivum and Lolium perenne. Widely distributed isolates in different lineages suggested that gene flow occurs across the regions. The highest level of gene flow (Nm = 0/7) were observed between USA and Japan populations and maximum inter population differentiation (G
= 0/5) were observed among rice and weeds populations of Iran. Analysis of Molecular Variance (AMOVA) revealed that variation within populations (88%) was significantly higher than among populations (12%).
ST It seems that selection due to host species was the primary factor determining population structure according to analysis of molecular variance.

Rice blast caused by the heterothallic ascomycete Magnaporthe oryzae is one of the most destructi... more Rice blast caused by the heterothallic ascomycete Magnaporthe oryzae is one of the most destructive
diseases in major rice growing regions of Iran. One hundred monoconidial isolates were collected from
infected rice plants showing typical blast symptoms on leaf, neck or panicle in Guilan and Mazandaran
provinces of Iran. Crossing experiments were performed with two standard hermaphrodite isolates GUY-11
(Mat1-2) and KA-7 (Mat1-1) on Rice Polish Agar (RPA) to determine the mating behavior and fertility
status. Among 100 isolates, only 55 (55%) isolates produced mature perithecia in crosses with GUY-11 and
the remaining isolates did not produce prethecia in crosses with both tester isolates and thus, were
completely sterile. As a result, all fertile isolates were male fertile (female sterile) and determined as Mat1-
1. Isolates were considered as fertile only if their perithecia produced viable ascospores. Also the mating
type idiomorph of each isolate was determined through a PCR-based assay using mating type specific
primers. Mating type of all isolates was determined as Mat1-1. The presence of only one mating type and
the absence of female fertile isolates indicate that sexual reproduction is absent in M. oryzae populations
from rice in North of Iran where approximately 75% of rice growing areas are located

Because of greater consumer awareness and concern regarding synthetic chemical additives, foods p... more Because of greater consumer awareness and concern regarding synthetic chemical additives, foods preserved with
natural additives have become popular.Thymol, SA and MeJA are naturally occurring compounds known as biocides.
Therefore invitro antifungal activity of the thymol, SA and MeJA were evaluated against postharvest pathogen Botrytis
cinerea.Thymol(0, 125, 250, 500and 1000 μll-1), MeJA (0, 10, 30 and 60μll-1) and SA (0, 25, 50 and 100 μll-1) were applied
via three methods (PDM (Paper Disc Method), adding to PDA and spraying) under in vitro condition.The results showed
thatthe most effective concentration of thymol was 250 μll-1 and 125μll-1 was the least effective concentration. The most
effective concentration of SA was 100 μll-1where the percentage of mycelia growth inhibition was 61.03 and adding to
PDA method produced more profound effects compared to PDM and spraying methods.No differences were found among
different MeJA levels and spraying method was the most effective method. As a general result, among all of these
treatments thymol in all concentrations (125, 250, 500 and 1000 μll-1) produced more profound effects and the least
effect on fungal growth were observed in SA with 25 and 50 μll-1 concentrations.
Keywords: Natural compounds, Fungal disease, Mycelia inhibition, Invitro

One hundred and thirty-five isolates of Fusariumoxysporum f. sp. dianthi(Fod) were obtained from ... more One hundred and thirty-five isolates of Fusariumoxysporum f. sp. dianthi(Fod) were obtained from diseased
samples of carnation from eleven provinces of Iran. All these isolates were used for pathogenicity test on susceptible
cultivar of Rendezvous. Among these, one hundred isolates were found to be pathogenic on carnation. Th e
pathogenic isolates were grouped in three vegetativ e compatibility groups (VCGs) using nitrate non-utilizing (nit)
mutant against a single non-pathogenic group. Withi n three VCGs, the biggest group (VCG 0021) contained
seventy-two members and VCG 0022 and VCG 0020 contained ten and three members, respectively.The Fod VCGs
representative mutants were then paired with nits o f different VCGs obtained from culture collection of
Dipartimento di Valorizzazione e ProtezionedelleRisorseAgroforestali (Di.Va.P.R.A) Patologiavegetale,Italy.Results
were shown that Iranian Fodisolates were belonged t o VCG0020, VCG0021 and VCG 0022. We found any wide
genetic diversity among IranianFod isolates. Also no correlation was observed between VCGs and pathogenicity
test among isolates. According to our knowledge this is the first study on the genetic diversity of
Fusariumoxysporumf.sp. dianthiin Iran and it is the first time these groups have been reported in Iran .

Strawberry as a non-climacteric and perishable fruit that is susceptible to fungal infections esp... more Strawberry as a non-climacteric and perishable fruit that is susceptible to fungal infections especially Botrytis cinerea. Therefore, its storage life is too short. Alternatives to the use of conventional fungicides are needed because of concerns about human health risks. Postharvest application of conventional fungicides to fruits is prohibited. The aim of this study was evaluation of nonchemical and safe compounds like methyl jasmonate. To prolonged fruit storage life and maintained quality and quantity characteristics of strawberry fruit. Therefore, MeJA at 4 concentration (0, 10, 30 and 60 µl
-1
) and 2 treatment methods (spray on fruits and paper disk method) in in vivo condition were used. Then quality characteristics of fruits (weight loss, pH, TA, TSS, vitamin C, anthocyanin, calcium, pectin, decay and sensory analyses) were evaluated. Treated fruits with MeJA had lower weight loss, pH, TSS and decay and higher TA, vitamin C, anthocyanin, calcium, pectin and fruit quality compared to controls. As a general result, all of treatments caused lower decay incidence and longer storability and improved fruit quality compared to controls. Among all treatment methods paper disk method had higher effectuality compared to spray method.