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Papers by viruthagiri thangavelu
Bioresources, Jul 18, 2010
The evaluation of medium components for lipase production by Pseudomonas fluorescens in submerged... more The evaluation of medium components for lipase production by Pseudomonas fluorescens in submerged batch fermentation was studied using Plackett-Burman experimental design. Twelve medium components with three dummy variables were studied in this experimental design. The most significant variables affecting lipase production were found to be glucose, olive oil, yeast extract, dipotassium hydrogen phosphate and ferrous sulphate heptahydrate. Maximum lipase activity of 3.32 U mL -1 and maximum cell mass concentration of 2.15 g L -1 was obtained in the 32 h of fermentation using the optimized medium under optimized conditions of 30 °C, with an initial pH of 7.0 at 120 rpm. Various unstructured kinetic models were analyzed to simulate the experimental values of cell growth, lipase activity and glucose concentration. Logistic model for cell growth, Luedeking-Piret model for lipase production and modified Luedeking-Piret model for substrate utilization were found to predict the fermentation...
Optimization of different parameters namely temperature, pH, incubation time and inoculum concent... more Optimization of different parameters namely temperature, pH, incubation time and inoculum concentration was carried out in the present investigation, to enhance the production of exopolysaccharide by Bacillus subtilis. A one factor at a time method was performed to obtain the central values for the further optimization by Response Surface Methodology (RSM). A Central Composite Design was developed to study the interactive effects of the four significant variables on EPS production. The maximum production was found to be 5.56 g L. The optimal parameter values were temperature 35C, pH 7, incubation time 72h and inoculum concentration 2 ml. This set of experiment gave 5.59 g L which was in good agreement with the predicted value of 5.62 g L.The structure of extracted EPS was investigated by FTIR spectroscopy showing the presence of sucrose units. The extracted polysaccharide could thus be an ecofriendly product which can be subjected to various industrial and pharmaceutical applications.
Chiang Mai University Journal of Natural Sciences
Microchemical Journal
Abstract In the present study, batch scale biodiesel production was carried out using castor oil ... more Abstract In the present study, batch scale biodiesel production was carried out using castor oil by alkali-catalyzed transesterification process was reported. Initially, the transesterification parameters like reaction time, catalyst concentration, reaction temperature and oil: methanol molar ratio on biodiesel production was optimized by conventional method followed by statistical based central composite design method. According to the optimized experimental results, the maximum of 94.9% FAME yield was obtained at 60 °C with 1.25% (w/v) KOH catalyst and 1:12 oil: methanol molar ratio for 60 min of reaction, which was in agreement with the predicted yield (93.7%). The purification of crude biodiesel was performed by simple evaporation and silica gel adsorption. The quality of fatty acid methyl ester was examined by Fourier Transform Infrared spectroscopy (FT-IR), Thin layer chromatography (TLC) and Gas Chromatography–Mass Spectrometry (GC–MS). The analytical results showed that significant quantity of methyl ester groups like ricinoleic, linolenic, palmitic acid and oleic acid were present in the biodiesel.
... Das,Optics Communications., 2008, 281, 2285. 18. P. Ramasamy., Journal of Crystal Growth., 20... more ... Das,Optics Communications., 2008, 281, 2285. 18. P. Ramasamy., Journal of Crystal Growth., 2008, 310, 1501. 19. S. Supriya and S. Kalainathan., International Journal of Chem Tech Research., 2011,3, 126. 20. S. Supriya, and ...
Journal of Bioprocessing & Biotechniques, 2012
Turkish Journal of Biology, Jun 3, 2013
Chemical Engineering World, 2007
Lebensmittel Wissenschaft Und Technologie, Jun 1, 2009
Chemical Engineering Research Bulletin, Apr 15, 2010
INTRODUCTION Phenol is one of the most widely used in the organic compounds in existence and is a... more INTRODUCTION Phenol is one of the most widely used in the organic compounds in existence and is a basic structural unit for a variety of synthetic organic compounds including agricultural chemicals and pesticides. Phenol is naturally found in decaying dead organic matters like rotting vegetables and in coal. The German chemist, Runge isolated phenol from coal tar in 1834 and named karbolsaure (coal-oil acid or carbolic acid), though its composition was not known until 1841. Phenol (hydroxy benzene) (Figure 1) is both a synthetically and naturally produced aromatic compound. It was first used in the raw state, as creosote, to prevent the weathering of railway ties and ships timber, and to reduce the odour of decomposition in sewage. At room temperature phenol is a translucent, colorless, crystalline mass, white powder or syrupy liquid when mixed with water. The crystals are hygroscopic and turn pink to red in air. Phenol has a sweet tar like odour and is soluble in alcohol, glycerol,...
Evaluation of twelve medium components on lipase production by Bacillus sphaericus MTCC511 in sub... more Evaluation of twelve medium components on lipase production by Bacillus sphaericus MTCC511 in submerged batch fermentation was studied using the Plackett-Burman statistical experimental design. The most significant variables affecting lipase production were found to be glucose, olive oil, peptone, NaCl and MnSO4 · H2O. Maximum lipase activity of 2.82 U ml–1 was obtained in the batch fermentation. The logistic model for cell growth, the Luedeking-Piret model for lipase production, and the modified Luedeking-Piret model for substrate utilization were capable of predicting the fermentation profile with high determination coefficient (R2) values of 0.9977, 0.9497 and 0.9962 respectively. The results indicate that this statistical method is an efficient tool in detecting the most significant medium components with a minimum number of experimental trials, and the unstructured models were able to describe fermentation kineticsmore effectively. The validity of the proposed model enables pre...
Bioresources, Jul 18, 2010
The evaluation of medium components for lipase production by Pseudomonas fluorescens in submerged... more The evaluation of medium components for lipase production by Pseudomonas fluorescens in submerged batch fermentation was studied using Plackett-Burman experimental design. Twelve medium components with three dummy variables were studied in this experimental design. The most significant variables affecting lipase production were found to be glucose, olive oil, yeast extract, dipotassium hydrogen phosphate and ferrous sulphate heptahydrate. Maximum lipase activity of 3.32 U mL -1 and maximum cell mass concentration of 2.15 g L -1 was obtained in the 32 h of fermentation using the optimized medium under optimized conditions of 30 °C, with an initial pH of 7.0 at 120 rpm. Various unstructured kinetic models were analyzed to simulate the experimental values of cell growth, lipase activity and glucose concentration. Logistic model for cell growth, Luedeking-Piret model for lipase production and modified Luedeking-Piret model for substrate utilization were found to predict the fermentation...
Optimization of different parameters namely temperature, pH, incubation time and inoculum concent... more Optimization of different parameters namely temperature, pH, incubation time and inoculum concentration was carried out in the present investigation, to enhance the production of exopolysaccharide by Bacillus subtilis. A one factor at a time method was performed to obtain the central values for the further optimization by Response Surface Methodology (RSM). A Central Composite Design was developed to study the interactive effects of the four significant variables on EPS production. The maximum production was found to be 5.56 g L. The optimal parameter values were temperature 35C, pH 7, incubation time 72h and inoculum concentration 2 ml. This set of experiment gave 5.59 g L which was in good agreement with the predicted value of 5.62 g L.The structure of extracted EPS was investigated by FTIR spectroscopy showing the presence of sucrose units. The extracted polysaccharide could thus be an ecofriendly product which can be subjected to various industrial and pharmaceutical applications.
Chiang Mai University Journal of Natural Sciences
Microchemical Journal
Abstract In the present study, batch scale biodiesel production was carried out using castor oil ... more Abstract In the present study, batch scale biodiesel production was carried out using castor oil by alkali-catalyzed transesterification process was reported. Initially, the transesterification parameters like reaction time, catalyst concentration, reaction temperature and oil: methanol molar ratio on biodiesel production was optimized by conventional method followed by statistical based central composite design method. According to the optimized experimental results, the maximum of 94.9% FAME yield was obtained at 60 °C with 1.25% (w/v) KOH catalyst and 1:12 oil: methanol molar ratio for 60 min of reaction, which was in agreement with the predicted yield (93.7%). The purification of crude biodiesel was performed by simple evaporation and silica gel adsorption. The quality of fatty acid methyl ester was examined by Fourier Transform Infrared spectroscopy (FT-IR), Thin layer chromatography (TLC) and Gas Chromatography–Mass Spectrometry (GC–MS). The analytical results showed that significant quantity of methyl ester groups like ricinoleic, linolenic, palmitic acid and oleic acid were present in the biodiesel.
... Das,Optics Communications., 2008, 281, 2285. 18. P. Ramasamy., Journal of Crystal Growth., 20... more ... Das,Optics Communications., 2008, 281, 2285. 18. P. Ramasamy., Journal of Crystal Growth., 2008, 310, 1501. 19. S. Supriya and S. Kalainathan., International Journal of Chem Tech Research., 2011,3, 126. 20. S. Supriya, and ...
Journal of Bioprocessing & Biotechniques, 2012
Turkish Journal of Biology, Jun 3, 2013
Chemical Engineering World, 2007
Lebensmittel Wissenschaft Und Technologie, Jun 1, 2009
Chemical Engineering Research Bulletin, Apr 15, 2010
INTRODUCTION Phenol is one of the most widely used in the organic compounds in existence and is a... more INTRODUCTION Phenol is one of the most widely used in the organic compounds in existence and is a basic structural unit for a variety of synthetic organic compounds including agricultural chemicals and pesticides. Phenol is naturally found in decaying dead organic matters like rotting vegetables and in coal. The German chemist, Runge isolated phenol from coal tar in 1834 and named karbolsaure (coal-oil acid or carbolic acid), though its composition was not known until 1841. Phenol (hydroxy benzene) (Figure 1) is both a synthetically and naturally produced aromatic compound. It was first used in the raw state, as creosote, to prevent the weathering of railway ties and ships timber, and to reduce the odour of decomposition in sewage. At room temperature phenol is a translucent, colorless, crystalline mass, white powder or syrupy liquid when mixed with water. The crystals are hygroscopic and turn pink to red in air. Phenol has a sweet tar like odour and is soluble in alcohol, glycerol,...
Evaluation of twelve medium components on lipase production by Bacillus sphaericus MTCC511 in sub... more Evaluation of twelve medium components on lipase production by Bacillus sphaericus MTCC511 in submerged batch fermentation was studied using the Plackett-Burman statistical experimental design. The most significant variables affecting lipase production were found to be glucose, olive oil, peptone, NaCl and MnSO4 · H2O. Maximum lipase activity of 2.82 U ml–1 was obtained in the batch fermentation. The logistic model for cell growth, the Luedeking-Piret model for lipase production, and the modified Luedeking-Piret model for substrate utilization were capable of predicting the fermentation profile with high determination coefficient (R2) values of 0.9977, 0.9497 and 0.9962 respectively. The results indicate that this statistical method is an efficient tool in detecting the most significant medium components with a minimum number of experimental trials, and the unstructured models were able to describe fermentation kineticsmore effectively. The validity of the proposed model enables pre...