Mukundraj Rathod | Swami Ramanand Teerth Marathwada University, Nanded (original) (raw)

Papers by Mukundraj Rathod

World Journal of Biology Pharmacy and Health Sciences, Feb 28, 2023

Nanoparticles are described as substances with at least one exterior dimension between 1 and 100 ... more Nanoparticles are described as substances with at least one exterior dimension between 1 and 100 nm. Nanoparticles can be produced by physical, chemical, or biological processes. Nanoparticle production techniques include chemical reduction, electrochemistry, photochemistry, and physical vapour condensation. However, some of these methods are very expensive and risky. The biological method for creating nanoparticles is simple, quick, inexpensive, dependable, and risk-free in comparison to these technologies. Our current study documented the green synthesis of copper nanoparticles using Ocimum sanctum (Tulsi) leaves extract and their antibacterial activity. We have used UV-vis spectroscopy to characterise copper nanoparticles. Ocimum sanctum leaves extract contained alkaloids, flavonoids, tannins, and saponins that might serve as capping and stabilising agents for the green synthesis of Ocimum sanctumcopper nanoparticles. In the form of a zone of growth inhibition, the copper nanoparticles demonstrated promising effects against all of the chosen bacteria. It was discovered that copper nanoparticle formation increased along with the reaction time of Ocimum sanctum leaves extract and copper sulphate. The inhibitory action of copper nanoparticles was found to increase along with the concentration of copper sulphate in solution. The effectiveness of different volumes of Ocimum sanctum leaves extract in reducing copper ions was assessed, and its effectiveness against Escherichia coli was confirmed.

World Journal of Advanced Engineering Technology and Sciences

Due to their excellent flavour and taste, Pleurotus species, which flourish in temperate and subt... more Due to their excellent flavour and taste, Pleurotus species, which flourish in temperate and subtropical environments, are well-liked among edible mushrooms. Oyster mushrooms could be grown on a variety of substrates due to their powerful enzymatic properties. Pleurotus sp. grows more easily, more profitably, and more nutrient-densely on a variety of organic waste raw materials and environmental factors. Sterilization of the substrate is one of the most crucial steps in oyster mushroom cultivation. There have been numerous reports of contaminated mushroom substrate. Suitable sterilization techniques must be used on the substrates. There are several treatments for mushroom substrate that can be used to manage the frequent moulds that infest edible mushrooms. Sterilization of the substrate is necessary to get rid of pathogenic and rival microorganisms and to encourage the mycelial growth of the desired mushroom species. The goal of the current study was to identify the best treatment ...

World Journal of Biology Pharmacy and Health Sciences

The oyster mushroom (Pleurotus florida) is one of the cultivated edible mushrooms and has certain... more The oyster mushroom (Pleurotus florida) is one of the cultivated edible mushrooms and has certain medicinal properties as well as economic and ecological values. Every year, massive amounts of ligno-cellulosic agricultural crop residues are produced worldwide, and not all of them are properly utilized. There are many ways of managing agricultural waste, one of which is mushroom cultivation. This study was conducted to compare the influence of selected consortiums of agricultural residues on the growth of Pleurotus florida. This species was cultivated on different combinations of substrates, viz., dried bread grass (Brachiaria brizantha), soybean straw, sawdust, rice straw, wheat straw, cotton straw, and sugarcane bagasse. Parameters such as height of stipe, girth of stipe, width of pileus, biological efficiency, and moisture content of fruiting bodies were evaluated. All the experiments were performed in triplicate. The supreme combination of substrates was found to be dried bread g...

World Journal of Advanced Research and Reviews

Microorganisms that love salt are known as halophiles, and they are found in both the archaeal an... more Microorganisms that love salt are known as halophiles, and they are found in both the archaeal and eubacterial domains of life. Halophiles do naturally occur in mesmeric saline habitats such as hypersaline lakes, salt pans, salt marshes, saline soils, and solar salterns. The group of microorganisms that live in a salty environment is known as the halophilic microbiome. This group includes bacteria, algae, fungi, viruses, and more. According to how much salt they can tolerate, halophiles are divided into three categories: mild (0.3-0.8 M), moderate (0.8-3.4 M), and extreme (3.4-5.1 M). Seawater already covers the majority of the surface of the Earth and has a salinity of roughly 0.6 M. The most numerous and adaptable microorganisms are therefore those that thrive in such environments. Due to the presence of carotenoids, many halophiles exhibit red-pigmented colonies. This pigment resembles that of tomatoes, red peppers, pink flamingos, and other similar foods. In the production of tr...

Alkaliphiles are widely distributed in all domains of life viz. eubacteria, archaea and eukarya. ... more Alkaliphiles are widely distributed in all domains of life viz. eubacteria, archaea and eukarya. Alkaliphilic microbes present in both near neutral and extreme habitats. These can grow vigorously and develop well at alkaline range of pH 8–10. About 63 alkaline lakes are present worldwide and there are only three mesmerizing crater lakes. Worldwide there are five alkaline soda lakes in Ethopia (Africa), six in Kenya and Tanzania (Africa), two in Russia (Asia) and Turkey (Asia), five in India (Asia), eleven in china (Asia), one in Mongolia (Asia), three in Hungary (Europe), eight in United States (North America), two in Canada (North America) and Mexico (North America), and one in Chile (South America) and Australia. Extracellular alkalozymes from alkaliphiles are known to have great industrial and environmental applications. In this mini-review selected industrially important alkalozymes from alkaliphiles and their geographical and phylogenetic distributions are discussed. Various ap...

Industrially important efficient alkaline protease producer was isolated from soil samples collec... more Industrially important efficient alkaline protease producer was isolated from soil samples collected from mango garden of S.R.T.M. University campus in Nanded district of Maharashtra and identified based on its morphological, microscopic and physiological characters, biochemical pattern and enzyme profile as Cyclobacterium sp. APM2. Production of alkaline protease was carried out in nutrient salt solution supplemented with 10 g/L defatted seed meal of soybean and 350 U/mL production of alkaline protease was recorded after 48 h. APM2 alkaline protease has shown remarkable catalytic efficiency at pH range 7-9and temperature range 30-60 oC as well. Therefore alkaline protease from Cyclobacterium sp. APM2 can be used in different biotechnological industries where hydrolysis of protein is carried out at alkaline pH and elevated range of temperature.

Alkaline protease producers were isolated from poultry farm waste and sea water samples and ident... more Alkaline protease producers were isolated from poultry farm waste and sea water samples and identified based on a polyphasic approach including 16S rRNA partial gene sequences as Bacillus aryabhattai P1, Bacillus firmus P2, Bacillus sp. P4, Bacillus cereus P5, Bacillus clausii P6, Alkalibacterium kapii MGR70, Halobacillus alkaliphilus MGR92, Alteromonas halophila MGR98 and Bacillus halodurans MGR125. Beside alkaline protease production, these isolates have also shown textile dye decolorization capability. Therefore two consortiums namely, PF1 and SW2 were developed to achieve maximum decolorization of the textile dyes, DR-81 and DO-34. The consortium SW2 has shown highest decolorization of Direct Red-81 (68.88 %) and Direct Orange-34 (70.78 %) at a concentration of 5 g/l after 144 h.

Extremophiles thrive in terrestrial hot springs, hydrothermal vents, glacial ices, saline lakes, ... more Extremophiles thrive in terrestrial hot springs, hydrothermal vents, glacial ices, saline lakes, solar saltern and acidic and alkaline niches; moreover a group of the extremophiles may grow in household hazardous waste, solvents containing cleaning and polishing chemicals, heavy metals contaminated sites, or in several other habitats such as aphotic, anoxic, athalassohaline and thalassohaline habitats that were previously considered inhospitable for life. Beside aforementioned extreme niches, extremophiles also dwell in poultry farm waste, salted fish, pickles, and many other food items. Extremophiles exhibit various applications in photographic, food, feed, dairy, detergent, pharmaceutical and leather industries. Bioactive compounds from them such as 2-phenylamine, beta-carotene, lycopene, phytoene, siderophores (iron chelators), organic acids, peptide antibiotics, caspase-1 inhibitors and lot of extremozymes are the topics of investigation amongst the researchers of extremophiles....

Industrially important amylase producer was isolated from soil samples of S.R.T.M.University camp... more Industrially important amylase producer was isolated from soil samples of S.R.T.M.University campus in Nanded district of Maharashtra and identified based on itsmorphological and microscopic characters as Penicillium sp. AFS3. Production of amylasewas carried out by using wheat and soybean husks in solid state fermentation. 81.80 U/mLproduction of amylase was recorded after 96 h. AFS3 amylase has shown remarkablecatalytic efficiency at pH range 4-6 and temperature range 30-50 oC as well. Thereforeamylase from Penicillium sp. AFS3 can be used in different biotechnological industrieswhere hydrolysis of starch is carried out at low pH and elevated range of temperature.

Efficient alkaline protease producing fungus was isolated from composite soil sample collected fr... more Efficient alkaline protease producing fungus was isolated from composite soil sample collected from the vicinity of Lonar Soda Lake ( Latitude 19° 58', Longitude 76° 36'). It was designated as LAF1 and identified as Rhizopus sp. on the basis of macro- and micromorphological features. Alkaline protease production was carried out in solid state fermentation system using whey and defatted seed meal of soybean and sunflower. Further extraction, partial purification and characterization of alkaline protease were carried out. After dialysis, the enzyme resulted in 1.10-fold increase which exhibited 14.41 U specific activity/mg of protein with 71.81 % yield. Molecular weight of the dialyzed alkaline protease was determined 40 kd. Catalytic activity of partially purified LAF1 alkaline protease was recorded at pH 10.0, temperature 55 o C and 14 mg/mL casein concentration. Lineweaver-Burk double reciprocal plot was constructed and Km and Vmax values were determined 2525.30 mg/mL and ...

The primary sources for occurrence of alkaline protease producers were found alkaline habitats vi... more The primary sources for occurrence of alkaline protease producers were found alkaline habitats viz. soil and water of soda lakes and deserts beside alkaline springs. Among bacteria, many efficient alkaline protease producers belong to the genus Bacillus. Alkaline proteases are the most versatile industrial enzymes that execute a variety of functions and have tremendous important biotechnological applications. Keeping in view the industrial importance of the alkaline proteases and their producers, research is now being focusing on the discovery of the alkaline proteases with novel intrinsic properties.

Efficient salt stable amylase producer was isolated by using starch agar medium containing10 % Na... more Efficient salt stable amylase producer was isolated by using starch agar medium containing10 % NaCl (pH 7.2) from soil samples collected from over irrigated and water logged sites ofKaleshwar farming area in Nanded district of Maharashtra. The salt stable amylase producerwas identified based on its morphological, microscopic and physiological characters,biochemical pattern and enzyme profile as Halobacillus sp. SSA1. Production of salt stableamylase was carried out in starch yeast extract medium containing 10 % NaCl. Production ofsalt stable amylase was recorded 2103.47 U/mL in cell free supernatant. The crude salt stableamylase has shown maximum catalytic efficiency at pH 7 and 4 oC temperature.

Unkeshwar hot spring of Maharashtra was explored for the isolation of industrially important effi... more Unkeshwar hot spring of Maharashtra was explored for the isolation of industrially important efficient thermostable lipase producers. These isolates were further identified based on their morphological characters, microscopic features, enzymatic profile and biochemical pattern as Bacillus megaterium APP8, Lysinibacillus sphaericus APP10, Paenibacillus alvei APP11, Pseudomonas fragii APP14, Bacillus badius APP23, Pseudomonas oleovorans APP30, Bacillus coagulans APP33, Bacillus subtilis APP36, Bacillus firmus APP39, and Microbacterium sp. APP41. Production of thermostable lipases was carried out at 50 o C temperature using lipase production medium. Partially purified lipases from these isolates exhibited specific activity in the range 22-68 U/mg. The partially purified lipases from these isolates have showed optimum catalytic activity in the range of temperature 40-60 o C and pH 8-9. Partially purified lipases from these isolates retained more than 70 % of residual activities even aft...

An efficient thermostable gelatinase producer was isolated from Unkeshwar hot spring of Nanded di... more An efficient thermostable gelatinase producer was isolated from Unkeshwar hot spring of Nanded district in Maharashtra. It was identified as Bacillus globisporus (APP11) by observing its morphological characters, microscopic features, biochemical pattern and physiological characterization. It showed luxuriant growth and thermostable gelatinase production at 50 C temperature. Effect of various agro-industrial residues on gelatinase production was determined. Maximum gelatinase production (675 ± 6.16 U/mg) from APP11 isolate was recorded after 24 h of time period using fish scale powder in the medium. Partially purified APP11 gelatinase was remarkably active at pH range 6 to 9 and temperature range 35 to 65 C. Km and Vmax values of partially purified APP11 gelatinase were 3.0 mg/mL and 1000 U/mg respectively. The APP11 originated gelatinase showed remarkable activity in presence of selected metal ions.

Ten different thermotolerent bacteria were isolated from terrestrial thermal spring of Unkeshwar ... more Ten different thermotolerent bacteria were isolated from terrestrial thermal spring of Unkeshwar in Nanded district of Maharashtra (India). These isolates were characterized by morphological characters, microscopic features, biochemical pattern and physiological attributes. These isolates were identified as Bacillus licheniformis (APP7), Bacillus megaterium (APP8), Actinobacillus hominis (APP9), Lysinibacillus sphaericus (APP10), Paenibacillus alvei (APP11), Bacillus simplex (APP12), Actinobacillus seminis (APP13), Pseudomonas fragii (APP14), Staphylococcus cohnii (APP15) and Streptococcus thermophilus (APP16). These isolates belonged to class Firmicutes and Gamma proteobacteria and showed production of biotechnologically important thermostable hydrolytic enzymes such as caseinase, amylase, gelatinase, urease and lipase.

World Journal of Biology Pharmacy and Health Sciences, Feb 28, 2023

Nanoparticles are described as substances with at least one exterior dimension between 1 and 100 ... more Nanoparticles are described as substances with at least one exterior dimension between 1 and 100 nm. Nanoparticles can be produced by physical, chemical, or biological processes. Nanoparticle production techniques include chemical reduction, electrochemistry, photochemistry, and physical vapour condensation. However, some of these methods are very expensive and risky. The biological method for creating nanoparticles is simple, quick, inexpensive, dependable, and risk-free in comparison to these technologies. Our current study documented the green synthesis of copper nanoparticles using Ocimum sanctum (Tulsi) leaves extract and their antibacterial activity. We have used UV-vis spectroscopy to characterise copper nanoparticles. Ocimum sanctum leaves extract contained alkaloids, flavonoids, tannins, and saponins that might serve as capping and stabilising agents for the green synthesis of Ocimum sanctumcopper nanoparticles. In the form of a zone of growth inhibition, the copper nanoparticles demonstrated promising effects against all of the chosen bacteria. It was discovered that copper nanoparticle formation increased along with the reaction time of Ocimum sanctum leaves extract and copper sulphate. The inhibitory action of copper nanoparticles was found to increase along with the concentration of copper sulphate in solution. The effectiveness of different volumes of Ocimum sanctum leaves extract in reducing copper ions was assessed, and its effectiveness against Escherichia coli was confirmed.

World Journal of Advanced Engineering Technology and Sciences

Due to their excellent flavour and taste, Pleurotus species, which flourish in temperate and subt... more Due to their excellent flavour and taste, Pleurotus species, which flourish in temperate and subtropical environments, are well-liked among edible mushrooms. Oyster mushrooms could be grown on a variety of substrates due to their powerful enzymatic properties. Pleurotus sp. grows more easily, more profitably, and more nutrient-densely on a variety of organic waste raw materials and environmental factors. Sterilization of the substrate is one of the most crucial steps in oyster mushroom cultivation. There have been numerous reports of contaminated mushroom substrate. Suitable sterilization techniques must be used on the substrates. There are several treatments for mushroom substrate that can be used to manage the frequent moulds that infest edible mushrooms. Sterilization of the substrate is necessary to get rid of pathogenic and rival microorganisms and to encourage the mycelial growth of the desired mushroom species. The goal of the current study was to identify the best treatment ...

World Journal of Biology Pharmacy and Health Sciences

The oyster mushroom (Pleurotus florida) is one of the cultivated edible mushrooms and has certain... more The oyster mushroom (Pleurotus florida) is one of the cultivated edible mushrooms and has certain medicinal properties as well as economic and ecological values. Every year, massive amounts of ligno-cellulosic agricultural crop residues are produced worldwide, and not all of them are properly utilized. There are many ways of managing agricultural waste, one of which is mushroom cultivation. This study was conducted to compare the influence of selected consortiums of agricultural residues on the growth of Pleurotus florida. This species was cultivated on different combinations of substrates, viz., dried bread grass (Brachiaria brizantha), soybean straw, sawdust, rice straw, wheat straw, cotton straw, and sugarcane bagasse. Parameters such as height of stipe, girth of stipe, width of pileus, biological efficiency, and moisture content of fruiting bodies were evaluated. All the experiments were performed in triplicate. The supreme combination of substrates was found to be dried bread g...

World Journal of Advanced Research and Reviews

Microorganisms that love salt are known as halophiles, and they are found in both the archaeal an... more Microorganisms that love salt are known as halophiles, and they are found in both the archaeal and eubacterial domains of life. Halophiles do naturally occur in mesmeric saline habitats such as hypersaline lakes, salt pans, salt marshes, saline soils, and solar salterns. The group of microorganisms that live in a salty environment is known as the halophilic microbiome. This group includes bacteria, algae, fungi, viruses, and more. According to how much salt they can tolerate, halophiles are divided into three categories: mild (0.3-0.8 M), moderate (0.8-3.4 M), and extreme (3.4-5.1 M). Seawater already covers the majority of the surface of the Earth and has a salinity of roughly 0.6 M. The most numerous and adaptable microorganisms are therefore those that thrive in such environments. Due to the presence of carotenoids, many halophiles exhibit red-pigmented colonies. This pigment resembles that of tomatoes, red peppers, pink flamingos, and other similar foods. In the production of tr...

Alkaliphiles are widely distributed in all domains of life viz. eubacteria, archaea and eukarya. ... more Alkaliphiles are widely distributed in all domains of life viz. eubacteria, archaea and eukarya. Alkaliphilic microbes present in both near neutral and extreme habitats. These can grow vigorously and develop well at alkaline range of pH 8–10. About 63 alkaline lakes are present worldwide and there are only three mesmerizing crater lakes. Worldwide there are five alkaline soda lakes in Ethopia (Africa), six in Kenya and Tanzania (Africa), two in Russia (Asia) and Turkey (Asia), five in India (Asia), eleven in china (Asia), one in Mongolia (Asia), three in Hungary (Europe), eight in United States (North America), two in Canada (North America) and Mexico (North America), and one in Chile (South America) and Australia. Extracellular alkalozymes from alkaliphiles are known to have great industrial and environmental applications. In this mini-review selected industrially important alkalozymes from alkaliphiles and their geographical and phylogenetic distributions are discussed. Various ap...

Industrially important efficient alkaline protease producer was isolated from soil samples collec... more Industrially important efficient alkaline protease producer was isolated from soil samples collected from mango garden of S.R.T.M. University campus in Nanded district of Maharashtra and identified based on its morphological, microscopic and physiological characters, biochemical pattern and enzyme profile as Cyclobacterium sp. APM2. Production of alkaline protease was carried out in nutrient salt solution supplemented with 10 g/L defatted seed meal of soybean and 350 U/mL production of alkaline protease was recorded after 48 h. APM2 alkaline protease has shown remarkable catalytic efficiency at pH range 7-9and temperature range 30-60 oC as well. Therefore alkaline protease from Cyclobacterium sp. APM2 can be used in different biotechnological industries where hydrolysis of protein is carried out at alkaline pH and elevated range of temperature.

Alkaline protease producers were isolated from poultry farm waste and sea water samples and ident... more Alkaline protease producers were isolated from poultry farm waste and sea water samples and identified based on a polyphasic approach including 16S rRNA partial gene sequences as Bacillus aryabhattai P1, Bacillus firmus P2, Bacillus sp. P4, Bacillus cereus P5, Bacillus clausii P6, Alkalibacterium kapii MGR70, Halobacillus alkaliphilus MGR92, Alteromonas halophila MGR98 and Bacillus halodurans MGR125. Beside alkaline protease production, these isolates have also shown textile dye decolorization capability. Therefore two consortiums namely, PF1 and SW2 were developed to achieve maximum decolorization of the textile dyes, DR-81 and DO-34. The consortium SW2 has shown highest decolorization of Direct Red-81 (68.88 %) and Direct Orange-34 (70.78 %) at a concentration of 5 g/l after 144 h.

Extremophiles thrive in terrestrial hot springs, hydrothermal vents, glacial ices, saline lakes, ... more Extremophiles thrive in terrestrial hot springs, hydrothermal vents, glacial ices, saline lakes, solar saltern and acidic and alkaline niches; moreover a group of the extremophiles may grow in household hazardous waste, solvents containing cleaning and polishing chemicals, heavy metals contaminated sites, or in several other habitats such as aphotic, anoxic, athalassohaline and thalassohaline habitats that were previously considered inhospitable for life. Beside aforementioned extreme niches, extremophiles also dwell in poultry farm waste, salted fish, pickles, and many other food items. Extremophiles exhibit various applications in photographic, food, feed, dairy, detergent, pharmaceutical and leather industries. Bioactive compounds from them such as 2-phenylamine, beta-carotene, lycopene, phytoene, siderophores (iron chelators), organic acids, peptide antibiotics, caspase-1 inhibitors and lot of extremozymes are the topics of investigation amongst the researchers of extremophiles....

Industrially important amylase producer was isolated from soil samples of S.R.T.M.University camp... more Industrially important amylase producer was isolated from soil samples of S.R.T.M.University campus in Nanded district of Maharashtra and identified based on itsmorphological and microscopic characters as Penicillium sp. AFS3. Production of amylasewas carried out by using wheat and soybean husks in solid state fermentation. 81.80 U/mLproduction of amylase was recorded after 96 h. AFS3 amylase has shown remarkablecatalytic efficiency at pH range 4-6 and temperature range 30-50 oC as well. Thereforeamylase from Penicillium sp. AFS3 can be used in different biotechnological industrieswhere hydrolysis of starch is carried out at low pH and elevated range of temperature.

Efficient alkaline protease producing fungus was isolated from composite soil sample collected fr... more Efficient alkaline protease producing fungus was isolated from composite soil sample collected from the vicinity of Lonar Soda Lake ( Latitude 19° 58', Longitude 76° 36'). It was designated as LAF1 and identified as Rhizopus sp. on the basis of macro- and micromorphological features. Alkaline protease production was carried out in solid state fermentation system using whey and defatted seed meal of soybean and sunflower. Further extraction, partial purification and characterization of alkaline protease were carried out. After dialysis, the enzyme resulted in 1.10-fold increase which exhibited 14.41 U specific activity/mg of protein with 71.81 % yield. Molecular weight of the dialyzed alkaline protease was determined 40 kd. Catalytic activity of partially purified LAF1 alkaline protease was recorded at pH 10.0, temperature 55 o C and 14 mg/mL casein concentration. Lineweaver-Burk double reciprocal plot was constructed and Km and Vmax values were determined 2525.30 mg/mL and ...

The primary sources for occurrence of alkaline protease producers were found alkaline habitats vi... more The primary sources for occurrence of alkaline protease producers were found alkaline habitats viz. soil and water of soda lakes and deserts beside alkaline springs. Among bacteria, many efficient alkaline protease producers belong to the genus Bacillus. Alkaline proteases are the most versatile industrial enzymes that execute a variety of functions and have tremendous important biotechnological applications. Keeping in view the industrial importance of the alkaline proteases and their producers, research is now being focusing on the discovery of the alkaline proteases with novel intrinsic properties.

Efficient salt stable amylase producer was isolated by using starch agar medium containing10 % Na... more Efficient salt stable amylase producer was isolated by using starch agar medium containing10 % NaCl (pH 7.2) from soil samples collected from over irrigated and water logged sites ofKaleshwar farming area in Nanded district of Maharashtra. The salt stable amylase producerwas identified based on its morphological, microscopic and physiological characters,biochemical pattern and enzyme profile as Halobacillus sp. SSA1. Production of salt stableamylase was carried out in starch yeast extract medium containing 10 % NaCl. Production ofsalt stable amylase was recorded 2103.47 U/mL in cell free supernatant. The crude salt stableamylase has shown maximum catalytic efficiency at pH 7 and 4 oC temperature.

Unkeshwar hot spring of Maharashtra was explored for the isolation of industrially important effi... more Unkeshwar hot spring of Maharashtra was explored for the isolation of industrially important efficient thermostable lipase producers. These isolates were further identified based on their morphological characters, microscopic features, enzymatic profile and biochemical pattern as Bacillus megaterium APP8, Lysinibacillus sphaericus APP10, Paenibacillus alvei APP11, Pseudomonas fragii APP14, Bacillus badius APP23, Pseudomonas oleovorans APP30, Bacillus coagulans APP33, Bacillus subtilis APP36, Bacillus firmus APP39, and Microbacterium sp. APP41. Production of thermostable lipases was carried out at 50 o C temperature using lipase production medium. Partially purified lipases from these isolates exhibited specific activity in the range 22-68 U/mg. The partially purified lipases from these isolates have showed optimum catalytic activity in the range of temperature 40-60 o C and pH 8-9. Partially purified lipases from these isolates retained more than 70 % of residual activities even aft...

An efficient thermostable gelatinase producer was isolated from Unkeshwar hot spring of Nanded di... more An efficient thermostable gelatinase producer was isolated from Unkeshwar hot spring of Nanded district in Maharashtra. It was identified as Bacillus globisporus (APP11) by observing its morphological characters, microscopic features, biochemical pattern and physiological characterization. It showed luxuriant growth and thermostable gelatinase production at 50 C temperature. Effect of various agro-industrial residues on gelatinase production was determined. Maximum gelatinase production (675 ± 6.16 U/mg) from APP11 isolate was recorded after 24 h of time period using fish scale powder in the medium. Partially purified APP11 gelatinase was remarkably active at pH range 6 to 9 and temperature range 35 to 65 C. Km and Vmax values of partially purified APP11 gelatinase were 3.0 mg/mL and 1000 U/mg respectively. The APP11 originated gelatinase showed remarkable activity in presence of selected metal ions.

Ten different thermotolerent bacteria were isolated from terrestrial thermal spring of Unkeshwar ... more Ten different thermotolerent bacteria were isolated from terrestrial thermal spring of Unkeshwar in Nanded district of Maharashtra (India). These isolates were characterized by morphological characters, microscopic features, biochemical pattern and physiological attributes. These isolates were identified as Bacillus licheniformis (APP7), Bacillus megaterium (APP8), Actinobacillus hominis (APP9), Lysinibacillus sphaericus (APP10), Paenibacillus alvei (APP11), Bacillus simplex (APP12), Actinobacillus seminis (APP13), Pseudomonas fragii (APP14), Staphylococcus cohnii (APP15) and Streptococcus thermophilus (APP16). These isolates belonged to class Firmicutes and Gamma proteobacteria and showed production of biotechnologically important thermostable hydrolytic enzymes such as caseinase, amylase, gelatinase, urease and lipase.