Victoria Osipova | Southern Scientific Center of Russian Academy of Science (original) (raw)

Papers by Victoria Osipova

Research paper thumbnail of The effectiveness of using lipid- and water-soluble analogues of vitamin E as antioxidant protectors for cryopreservation of sperm of sterlet (Acipenser ruthenus) and Russian sturgeon (Acipenser gueldenstaedtii)

E3S Web of Conferences

The aim of the research was to comparative study the effectiveness of synthetic antioxidants – li... more The aim of the research was to comparative study the effectiveness of synthetic antioxidants – lipid-soluble (butylated hydroxytoluene, BHT) and water–soluble (trolox) analogues of vitamin E for the cryopreservation of Russian sturgeon and sterlet sperm in the presence of the modified Stein’s medium. The ability of phenolic antioxidants to reduce the level of lipid peroxidation of sturgeon sperm, beneficial effect on the motility of thawed sperm has been shown. The addition of lipid- and water-soluble antioxidants to a basic cryomedim increased the fertilization rate of Russian sturgeon and sterlet sperm cells about 10% compared to the control experiment. The insignificant protective effect of these antioxidants presumably is explained by the use of a cryogenic medium with additive of toxic DMSO, therefore, it is necessary to develop a new composition of the cryo-medium with a minimum concentration of DMSO.

Research paper thumbnail of Anti-Inflammatory Activity of Soluble Epoxide Hydrolase Inhibitors Based on Selenoureas Bearing an Adamantane Moiety

International Journal of Molecular Sciences

The inhibitory potency of the series of inhibitors of the soluble epoxide hydrolase (sEH) based o... more The inhibitory potency of the series of inhibitors of the soluble epoxide hydrolase (sEH) based on the selenourea moiety and containing adamantane and aromatic lipophilic groups ranges from 34.3 nM to 1.2 μM. The most active compound 5d possesses aliphatic spacers between the selenourea group and lipophilic fragments. Synthesized compounds were tested against the LPS-induced activation of primary murine macrophages. The most prominent anti-inflammatory activity, defined as a suppression of nitric oxide synthesis by LPS-stimulated macrophages, was demonstrated for compounds 4a and 5b. The cytotoxicity of the obtained substances was studied using human neuroblastoma and fibroblast cell cultures. Using these cell assays, the cytotoxic concentration for 4a was 4.7–18.4 times higher than the effective anti-inflammatory concentration. The genotoxicity and the ability to induce oxidative stress was studied using bacterial lux-biosensors. Substance 4a does not exhibit genotoxic properties, ...

Research paper thumbnail of Activity of Hydroxy Derivatives of Chalcones toward Superoxide Anion Radical

Doklady Chemistry

Trapping activity of new hydroxy derivatives of chalcones toward electrochemically generated supe... more Trapping activity of new hydroxy derivatives of chalcones toward electrochemically generated superoxide anion radical (\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${\\text{O}}_{2}^{{\\bullet \\, - }}$$\\end{document}) in a xanthine/xanthine oxidase enzymatic system upon reduction of nitro blue tetrazolium and during non-enzymatic quinoid oxidation of adrenaline in alkaline medium has been studied. Antiradical activity of new hydroxy derivatives of chalcones toward \\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${\\text{O}}_{2}^{{\\bullet \\, - }}$$\\end{document} has been established for the first time. Reaction with electrochemically generated \\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${\\text{O}}_{2}^{{\\bullet \\, - }}$$\\end{document} and higher antiradical activity under adrenaline autooxidation conditions in alkaline medium has been revealed for chalcone containing 2,6-di-tert-butylphenol fragment. Chalcone with two non-shielded OH groups showed the highest trapping activity in xanthine/xanthine oxidase enzymatic system and SOD-protective activity. The inhibiting activity of hydroxy derivatives of chalcones toward \\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${\\text{O}}_{2}^{{\\bullet \\, - }}$$\\end{document} agrees well with in silico prediction and indicates their ability to decrease probability of oxidative stress development. Correlation has been found between the ability of the studied chalcones to consume superoxide anion radical and inhibiting activity in model systems of peroxide oxidation of oleic acid and tilapia liver lipids.

Research paper thumbnail of Antioxidant Activity and Cytotoxicity of Aromatic Oligosulfides

Molecules

Natural or synthetic antioxidants with biomimetic fragments protect the functional and structural... more Natural or synthetic antioxidants with biomimetic fragments protect the functional and structural integrity of biological molecules at a minimum concentration, and may be used as potential chemotherapeutic agents. This paper is devoted to in silico and in vitro evaluation of the antioxidant and cytotoxic properties of synthetic analogues of natural compounds—aromatic oligosulfides. The antiradical and SOD-protective activity of oligosulfides was demonstrated in the reaction with O2–• generated in enzymatic and non-enzymatic systems. It was found that phenol-containing disulfides significantly reduced the accumulation level of hydroperoxides and secondary carbonyl thiobarbituric acid reactive substances, which are primary products of oleic acid peroxidation. The antioxidant efficiency of bis(3,5-di-tert-butyl-4-hydroxyphenyl) disulfide increased over time due to the synergistic action of the 2,6-di-tert-butylphenol fragment and the disulfide linker. The highest cytotoxicity on the A-...

Research paper thumbnail of Study of Antioxidant Activity of New Compounds with 1,3-Thiazin-2,6-Dione and Pyrrolidine Fragments

Doklady Chemistry, 2021

Antioxidant activity of new compounds with 1,3-diazin-2,6-dione and pyrrolidine fragments in comp... more Antioxidant activity of new compounds with 1,3-diazin-2,6-dione and pyrrolidine fragments in comparison with 2,6-di-tert-butyl-4-mercaptophenol has been studied in the model system of adrenaline autooxidation in alkaline medium in DPPH, NBT, and CUPRAC tests. In the series of studied compounds, a leader has been revealed, which shows moderate antiradical activity toward DPPH radical, prevents accumulation of toxic products of adrenaline quinoid oxidation, and exhibits higher efficiency as one-electron Cu2+ reducing reagent as compared with known antioxidants, trolox and 2,6-di-tert-butyl-4-mercaptophenol.

Research paper thumbnail of Evaluation of antioxidant properties of new functionally substituted aryl carbamates

AIP Conference Proceedings, 2022

The antioxidative activity of new carbamate derivatives has been compared by studying their influ... more The antioxidative activity of new carbamate derivatives has been compared by studying their influence on the generation of superoxide anion radical O-• in the enzyme/substrate system xanthine/xanthine oxidase, on adrenaline autoxidation in an alkaline medium and on the level of lipid peroxidation in bester liver. Investigated aryl carbamates showed the inhibition of the nitroblue tetrazolium reduction by O-• generated in the xanthine/xanthine oxidase system, the accumulation of adrenaline oxidation reaction products both in the absence and in the presence of tilapia liver cytosol and the level of carbonyl oxidation by-products, which can react with thiobarbituric acid. These results showed that the new functionally substituted aryl carbamates possess a potent protective effect both preventing the development of oxidative stress and inhibiting oxidative processes under conditions of the development of oxidative stress.

Research paper thumbnail of Porphyrins with Phenolic Fragments at the Periphery of the Macrocycle as Perspective Antioxidants, Cytoprotectors and Heavy Metal Scavengers

Chemistry of Heterocyclic Compounds, 2021

The past few decades witnessed intense research devoted to the antioxidant activity of porphyrins... more The past few decades witnessed intense research devoted to the antioxidant activity of porphyrins. We review herein recent results of the studies of a series of porphyrins with antioxidant phenol groups in meso positions and an in vitro and in vivo study of their protective effects on free radical-induced damage of biological substances. The antioxidant activity of para-hydroxy-substituted meso-tetraphenylporphines and their metal complexes are summarized. The radical scavenging activity of porphyrins in relation to toxic species, including the highly reactive oxygen species is considered. The protective effects of these porphyrins have been proved in model reactions as well as in vitro and in vivo studies. A special attention is addressed to the polyfunctional porphyrins capable of preventing the toxicity of heavy metals due to antioxidant as well as scavenging ability.

Research paper thumbnail of Antioxidant activity of some organosulfur compounds in vitro

Arabian Journal of Chemistry, 2021

The medicinal properties of many natural plant products are related with the antioxidant action o... more The medicinal properties of many natural plant products are related with the antioxidant action of their constituents-oligosulfides and polyphenols. To increase the effectiveness of the antioxidant effect in lipid peroxidation and for reducing of undesirable toxic effects the combination in one molecule of several functional groups that act by various mechanisms seems one profitable. The goal of present study was to determine the antioxidant properties of synthetic sterically hindered 2,6-di-tert-butyl-4-mercaptophenol (1), bis(3,5-di-tert-butyl-4-hydroxyphenyl)disulfide (2) in comparison with disulfides containing in plant products: diphenyl disulfide (3), dibenzyl disulfide (4), dibutyl disulfide (5), di-tert-butyl disulfide (6), diallyl disulfide (7), methyl propyl trisulfide (8). Antioxidant properties were investigated by various assays including DPPH radical scavenging, Cu 2+ ion reduction (CUPRAC-test), model reactions of peroxidation of cis-9-octadecenoic (oleic) acid and lipids from the liver of Russian sturgeon in vitro as well as metal chelating activity. It was found that bis(3,5-di-tert-butyl-4-hydroxyphenyl) disulfide demonstrated both high DPPH radical scavenging activity and TEAC CUPRAC value and significantly decreased the level of primary products of lipid peroxidation (LPO)-lipid hydroperoxides (LOOH) as well as secondary productscarbonyl compounds which form colored complexes with thiobarbituric acid (TBARS). All sulfur compounds demonstrated the inhibitory activity in oleic acid peroxidation except dibutyl disulfide. The maximum decrease of LOOH and TBARS in lipid peroxidation was detected for bis(3,5-di-tertbutyl-4-hydroxyphenyl) disulfide. In the case of long-term LPO of liver lipids the antioxidant activity of all sulfur-containing compounds except diphenyl disulfide that exhibit pro-oxidant activity was ascertained. The efficiency of the antioxidant action of most compounds was decreased with time. Compound 2 was the only exception for which the increase in the efficiency of antioxidant

Research paper thumbnail of Synthesis and antioxidant activity of new hydroxy derivatives of chalcones

Russian Chemical Bulletin, 2020

New hydroxy derivatives of chalcones obtained via a condensation of 3-acetyl-2H-chromen-2-one wit... more New hydroxy derivatives of chalcones obtained via a condensation of 3-acetyl-2H-chromen-2-one with 2,4-dihydroxy-and 4-hydroxy-benzaldehydes and via an aldol crotonic condensation of methyl N-(4-acetylphenyl)carbamate with 4-hydroxy-3,5-di(tert-butyl)benzaldehyde have been isolated and characterized. A wide range of promising biological activity of the synthesized compounds, including the radical scavenging and antioxidant ones, was predicted by the in silico method. The anti-and pro-oxidant activities in model systems of peroxidation of cis-9-octadecenoic (oleic) acid and lipids from the liver homogenate of tilapia were estimated in vitro. The presence of sterically hindered hydroxy groups and a chromene moiety provides the prolonged antioxidant activity of new hydroxy derivatives of chalcones.

Research paper thumbnail of Cryoprotective activity of phosphorus-containing phenol

Cryobiology, 2020

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Research paper thumbnail of The Effect of Tin Compounds on the Lipid Peroxidation Level of Russian Sturgeon Fresh and Cryopreserved Sperm

Environmental Research, Engineering and Management, 2020

The effect of organotin compounds (OTs) on the accumulation of the lipid peroxidation (LPO) carbo... more The effect of organotin compounds (OTs) on the accumulation of the lipid peroxidation (LPO) carbonyl by-products, which react with thiobarbituric acid (TBARS) in fresh and cryopreserved sperm of Russian sturgeon, was studied. It was found that incubation (1 hour) of Russian sturgeon sperm with OTs (CH3SnCl3, (CH3)2SnCl2, (CH3)3SnCl, (n-C4H9)2SnCl2, (n-C4H9)3SnCl, (C6H5)2SnCl2, (C6H5)3SnCl) in concentration 0.1 mM led to the promotion of the accumulation of TBARS in native semen. Dimethyl- (DMT) and diphenyltin dichlorides (DPT) exhibited the greatest promoting activity, and the LPO level of both native and cryopreserved sperm of Russian sturgeon, including those in modified Stein's cryomedium, increased in the presence of these compounds. It was found that Russian sturgeon's cryopreserved sperm had lower sensitivity to the promotion of sperm LPO by DMT and DPT compared with the native sperm. The protective effect of Stein’s cryomedium decreased in the presence of the studied...

Research paper thumbnail of In silico and in vitro evaluation of the biological activity of some organicsulfur-containing compounds

TURKISH JOURNAL OF CHEMISTRY, 2019

It was predicted, using the Prediction of Activity Spectra for Substances online software, that o... more It was predicted, using the Prediction of Activity Spectra for Substances online software, that organic sulfur-containing compounds 2,6-di-tert-butyl-4-mercaptophenol (1), 2,6-di-tert-butyl-4-(3-mercaptopropyl)phenol (2), bis-3-[3,5-di-tert-butyl-4-hydroxyphenyl)propyl] sulfide (3), bis(3,5-di-tert-butyl-4-hydroxyphenyl) disulfide (4), diphenyl disulfide (5), dibenzyl disulfide (6), dibutyl disulfide (7), di-tert-butyl disulfide (8), diallyl disulfide (9), and methyl propyl trisulfide (10) could possess antioxidant activity and act as active scavengers of oxygen metabolites. The antiradical activity of these species was analyzed and it was shown that they were able to react with electrochemically generated the superoxide anion radical. The rate constants for reactions of 1-10 with the superoxide anion radical were calculated. The effect of the organic sulfur compounds on the rate of generation O −• 2 on adrenaline oxidation in an alkaline medium and on the ability of the biopreparation, based on the liver of Russian sturgeon, to deactivate O −• 2 was studied. The effect of the organic sulfur compounds on the rate of O −• 2 generation in the model system of the quinoid oxidation of adrenaline in an alkaline medium and on the ability of the biopreparation to deactivate O −• 2 was studied. It was found that O −• 2 generation rate decreased in the presence of the studied compounds, evidencing for their antiradical activity. Increase of superoxide dismutation activity of the biopreparation in the presence of compounds 1-10 was also shown, and this fact could indicate their ability to increase the antioxidant status and decrease the probability of oxidative stress development. It was found that compounds 1-10 did not inhibit enzyme lipoxygenase, which indicated the absence of antiinflammatory activity. The results of the forecast were consistent with the data of the experimental studies.

Research paper thumbnail of Application of new phenolic antioxidants for cryopreservation of sturgeon sperm

Cryobiology, 2016

Heterocyclic derivatives of butylated hydroxytoluene (BHT) were studied as cryoprotectants of the... more Heterocyclic derivatives of butylated hydroxytoluene (BHT) were studied as cryoprotectants of the basic media for cryopreservation of the Russian sturgeon sperm. Rates of lipid peroxidation of sturgeon sperm before and after cryopreservation were reduced in the presence of the studied compounds, exceeding the effects of BHT and water-soluble analogue of vitamin E, trolox. The most efficient antioxidant has the effective concentration of 0.1 mM. Novel antioxidant agents as cryomedium supplements not only reduced the level of lipid peroxidation, but also enhanced the translational motility of the sperm of the Russian sturgeon after defrosting.

Research paper thumbnail of Protecting Effect of the Free Base Porphyrins on the Rate of Hydrogen Peroxide Decomposition by Hemolysate of Human Blood Erythrocytes in the Presence of Mercury and Tin Compounds

Macroheterocycles, 2011

An influence of meso-tetraphenylporphyrin (TPPH 2) and its derivatives, i.e. meso-tetrakis(3,5-di... more An influence of meso-tetraphenylporphyrin (TPPH 2) and its derivatives, i.e. meso-tetrakis(3,5-di-tert-butyl-4hydroxyphenyl)porphyrin (R 4 PH 2), meso-tetra(4-sulfonatophenyl)porphine ((HO 3 S) 4 TPPН 2), in the presence of mercury and tin compounds on the rate of hydrogen peroxide decomposition by hemolysate of laundered human blood erythrocytes was studied. The decrease of the decomposition rate of hydrogen peroxide by erythrocytes in the presence of TPPH 2 and mercury (HgCl 2 , CH 3 HgI, Hg(NO 3) 2 •H 2 O) or tin ((CH 3) 2 SnCl 2 , (n-C 4 H 9) 3 SnCl, (n-C 4 H 9) 2 SnCl 2 , (C 6 H 5) 3 SnCl, (C 6 H 5) 2 SnCl 2)) compounds was ascertained. (n-C 4 H 9) 3 SnCl, which is used as biocidal additive in paints, is the most toxic in this row; the addition of this compound decreases the rate of H 2 O 2 decomposition by 70%. The influence of organotin compounds on the reaction rate depends on the nature of organic group and, in the case of butyl and methyl derivatives, on the quantity of these groups. The influence of addition of the extremely toxic methylmercury salts on the decomposition rate of hydrogen peroxide does not differ from the influence of inorganic mercury salts. The decrease of the H 2 O 2 decomposition rate in the presence of tin and mercury compounds may be connected with inhibition of catalase enzyme. At these conditions, an excess of hydrogen peroxide is formed, what brings to the change of permeability of biomembranes and oxidative stress. When (HO 3 S) 4 TPPН 2 is added in erythrocytes hemolysate the rate of enzymatic reaction is similar to control one. So an inclusion of sulfo group on the periphery of porphyrin molecule TPPH 2 eliminates the decrease of H 2 O 2 decomposition rate. The UV-vis spectrum of (HO 3 S) 4 TPPН 2 indicates the broadening and a small hypsochromic shift of the Soret band (5 nm) if compared with that of TPPH 2. Absorption intensity at the Q-band region (500-600 nm) is also decreased. An addition of R 4 PH 2 to erythrocytes hemolysate, containing tin compounds, causes an increase of catalase activity, thus the decrease of H 2 O 2 decomposition rate by toxicants is eliminated. Maximum efficiency of R 4 PH 2 additives was established in the presence of (n-C 4 H 9) 2 SnCl 2. An addition of TPPH 2 leads to the further reduce of the rate of H 2 O 2 decomposition by erythrocytes hemolysate in the presence of (CH 3) 3 SnCl and (n-C 4 H 9) 3 SnCl. The additives of sulfoporphyrin in hemolysate, containing CH 3 HgI, (CH 3) 3 SnCl, Hg(NO 3) 2 , HgCl 2 , results in increasing of H 2 O 2 decomposition rate. Water-soluble (HO 3 S) 4 TPPН 2 decreases the negative impact of tin and mercury compounds to the rate of enzymatic reactions. An increase of the rate of hydrogen peroxide decomposition by hemolysate of the laundered human blood erythrocytes, caused by R 4 PH 2 in the presence of organotin derivatives, contributes to the antioxidant status of blood erythrocytes in the conditions of the toxicants action.

Research paper thumbnail of Cryoprotective effect of phosphorous-containing phenolic anti-oxidant for the cryopreservation of beluga sperm

Cryobiology, 2014

A cryoprotective effect of an addition of a new synthetic antioxidant - a representative of phosp... more A cryoprotective effect of an addition of a new synthetic antioxidant - a representative of phosphorus-containing sterically hindered phenols is presented. The efficiency of the compound was shown to exceed the effect of lipid-soluble antioxidants butylated hydroxytoluene (BHT) and trolox in the conditions of cryopreservation of beluga sperm in the presence of the modified Stein's medium. It was shown that the level of carbonyl oxidation by-products, which can react with thiobarbituric acid (TBARS), in beluga sperm was inversely proportional to the motility time of sperm cells. The fertility of beluga sperm increased 2 times upon the addition of phosphorus-containing phenol to a modified Stein's medium. The prospects of the new antioxidant application to improve cryoresistance of beluga sperm in the conditions of cryopreservation for its efficient protection from the peroxidation processes are discussed.

Research paper thumbnail of Redox properties of novel pyrrolidine derivatives containing sterically hindered phenol fragment

Russian Journal of Electrochemistry, 2011

ABSTRACT The method of cyclic voltammetry on a platinum electrode in acetonitrile is used to stud... more ABSTRACT The method of cyclic voltammetry on a platinum electrode in acetonitrile is used to study the redox properties of a number of novel pyrrolidine derivatives containing a sterically hindered phenol fragment. It is found that the obtained derivatives of (3,5-di-tert-butyl-4-hydroxyphenyl)pyrrolidine-2-carboxylic acid are irreversibly oxidized in two stages with formation of a phenoxy radical. The structural analogue containing no phenyl fragment is irreversibly oxidized under similar conditions in a single stage. Formation of a stable phenoxy radical from synthesized (3,5-di-tert-butyl-4-hydroxyphenyl)pyrrolidines under PbO2 oxidation is confirmed by ESR spectroscopy. Application of the synthesized compounds as antioxidant agents has been suggested.

Research paper thumbnail of Radical scavenging activity of sterically hindered catecholate and o-amidophenolate complexes of LSbVPh3 type

Journal of Organometallic Chemistry, 2011

The radical scavenging effect of the substituted catecholates (1–3, 6) and o-amidophenolates (4, ... more The radical scavenging effect of the substituted catecholates (1–3, 6) and o-amidophenolates (4, 5) of triphenylantimony(V) in reactions with DPPH• radical and in a process of oleic acid peroxidation was studied in details. Complexes 1–6 show the high activity in radical scavenging reactions with DPPH• radical leading to disappearance of radical species. Complexes were demonstrated to be high-efficient inhibitors of

Research paper thumbnail of Antioxidant Activity and Toxic Effects of Phosphorus-Containing Derivatives of 2,6-Di-tert-Butylphenol in silico and in vitro, in vivo

Environmental Research, Engineering and Management, 2019

By using PASS online web resource, it was predicted that phosphorus-containing derivatives of 2,6... more By using PASS online web resource, it was predicted that phosphorus-containing derivatives of 2,6-di-tert-butylphenol had high probability to possess antioxidant activity and to act as active oxygen metabolite "traps" and heavy metal antidotes. The values of the acute toxicity index of phosphorus-containing phenols for rats, aquatic organisms, and bioaccumulation factor, calculated by the GUSAR programme, indicate potentially low toxicity. The results of the forecast are comparable with the data of experimental studies in vitro and in vivo experiments.

Research paper thumbnail of Synthesis and antioxidant activity of new hydroxy derivatives of chalcones

New hydroxy derivatives of chalcones obtained via a condensation of 3-acetyl-2H-chromen-2-one wit... more New hydroxy derivatives of chalcones obtained via a condensation of 3-acetyl-2H-chromen-2-one with 2,4-dihydroxy-and 4-hydroxy-benzaldehydes and via an aldol crotonic condensation of methyl N-(4-acetylphenyl)carbamate with 4-hydroxy-3,5-di(tert-butyl)benzaldehyde have been isolated and characterized. A wide range of promising biological activity of the synthesized compounds, including the radical scavenging and antioxidant ones, was predicted by the in silico method. The anti-and pro-oxidant activities in model systems of peroxidation of cis-9-octadecenoic (oleic) acid and lipids from the liver homogenate of tilapia were estimated in vitro. The presence of sterically hindered hydroxy groups and a chromene moiety provides the prolonged antioxidant activity of new hydroxy derivatives of chalcones.

Research paper thumbnail of The Effect of Tin Compounds on the Lipid Peroxidation Level of Russian Sturgeon Fresh and Cryopreserved Sperm The Effect of Tin Compounds on the Lipid Peroxidation Level of Russian Sturgeon Fresh and Cryopreserved Sperm

The effect of organotin compounds (OTs) on the accumulation of the lipid peroxidation (LPO) carbo... more The effect of organotin compounds (OTs) on the accumulation of the lipid peroxidation (LPO) carbonyl by-products , which react with thiobarbituric acid (TBARS) in fresh and cryopreserved sperm of Russian sturgeon, was studied. It was found that incubation (1 hour) of Russian sturgeon sperm with OTs (CH 3 SnCl 3 , (CH 3) 2 SnCl 2 , (CH 3) 3 SnCl, (n-C 4 H 9) 2 SnCl 2 , (n-C 4 H 9) 3 SnCl, (C 6 H 5) 2 SnCl 2 , (C 6 H 5) 3 SnCl) in concentration 0.1 mM led to the promotion of the accumulation of TBARS in native semen. Dimethyl-(DMT) and diphenyltin dichlorides (DPT) exhibited the greatest promoting activity, and the LPO level of both native and cryopreserved sperm of Russian sturgeon, including those in modified Stein's cryomedium, increased in the presence of these compounds. It was found that Russian sturgeon's cryopreserved sperm had lower sensitivity to the promotion of sperm LPO by DMT and DPT compared with the native sperm. The protective effect of Stein's cryomedium decreased in the presence of the studied OTs. The results suggest that accumulation of OTs by gonad of fish is another stress factor affecting the cell productivity in the cryopreservation process.

Research paper thumbnail of The effectiveness of using lipid- and water-soluble analogues of vitamin E as antioxidant protectors for cryopreservation of sperm of sterlet (Acipenser ruthenus) and Russian sturgeon (Acipenser gueldenstaedtii)

E3S Web of Conferences

The aim of the research was to comparative study the effectiveness of synthetic antioxidants – li... more The aim of the research was to comparative study the effectiveness of synthetic antioxidants – lipid-soluble (butylated hydroxytoluene, BHT) and water–soluble (trolox) analogues of vitamin E for the cryopreservation of Russian sturgeon and sterlet sperm in the presence of the modified Stein’s medium. The ability of phenolic antioxidants to reduce the level of lipid peroxidation of sturgeon sperm, beneficial effect on the motility of thawed sperm has been shown. The addition of lipid- and water-soluble antioxidants to a basic cryomedim increased the fertilization rate of Russian sturgeon and sterlet sperm cells about 10% compared to the control experiment. The insignificant protective effect of these antioxidants presumably is explained by the use of a cryogenic medium with additive of toxic DMSO, therefore, it is necessary to develop a new composition of the cryo-medium with a minimum concentration of DMSO.

Research paper thumbnail of Anti-Inflammatory Activity of Soluble Epoxide Hydrolase Inhibitors Based on Selenoureas Bearing an Adamantane Moiety

International Journal of Molecular Sciences

The inhibitory potency of the series of inhibitors of the soluble epoxide hydrolase (sEH) based o... more The inhibitory potency of the series of inhibitors of the soluble epoxide hydrolase (sEH) based on the selenourea moiety and containing adamantane and aromatic lipophilic groups ranges from 34.3 nM to 1.2 μM. The most active compound 5d possesses aliphatic spacers between the selenourea group and lipophilic fragments. Synthesized compounds were tested against the LPS-induced activation of primary murine macrophages. The most prominent anti-inflammatory activity, defined as a suppression of nitric oxide synthesis by LPS-stimulated macrophages, was demonstrated for compounds 4a and 5b. The cytotoxicity of the obtained substances was studied using human neuroblastoma and fibroblast cell cultures. Using these cell assays, the cytotoxic concentration for 4a was 4.7–18.4 times higher than the effective anti-inflammatory concentration. The genotoxicity and the ability to induce oxidative stress was studied using bacterial lux-biosensors. Substance 4a does not exhibit genotoxic properties, ...

Research paper thumbnail of Activity of Hydroxy Derivatives of Chalcones toward Superoxide Anion Radical

Doklady Chemistry

Trapping activity of new hydroxy derivatives of chalcones toward electrochemically generated supe... more Trapping activity of new hydroxy derivatives of chalcones toward electrochemically generated superoxide anion radical (\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${\\text{O}}_{2}^{{\\bullet \\, - }}$$\\end{document}) in a xanthine/xanthine oxidase enzymatic system upon reduction of nitro blue tetrazolium and during non-enzymatic quinoid oxidation of adrenaline in alkaline medium has been studied. Antiradical activity of new hydroxy derivatives of chalcones toward \\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${\\text{O}}_{2}^{{\\bullet \\, - }}$$\\end{document} has been established for the first time. Reaction with electrochemically generated \\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${\\text{O}}_{2}^{{\\bullet \\, - }}$$\\end{document} and higher antiradical activity under adrenaline autooxidation conditions in alkaline medium has been revealed for chalcone containing 2,6-di-tert-butylphenol fragment. Chalcone with two non-shielded OH groups showed the highest trapping activity in xanthine/xanthine oxidase enzymatic system and SOD-protective activity. The inhibiting activity of hydroxy derivatives of chalcones toward \\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${\\text{O}}_{2}^{{\\bullet \\, - }}$$\\end{document} agrees well with in silico prediction and indicates their ability to decrease probability of oxidative stress development. Correlation has been found between the ability of the studied chalcones to consume superoxide anion radical and inhibiting activity in model systems of peroxide oxidation of oleic acid and tilapia liver lipids.

Research paper thumbnail of Antioxidant Activity and Cytotoxicity of Aromatic Oligosulfides

Molecules

Natural or synthetic antioxidants with biomimetic fragments protect the functional and structural... more Natural or synthetic antioxidants with biomimetic fragments protect the functional and structural integrity of biological molecules at a minimum concentration, and may be used as potential chemotherapeutic agents. This paper is devoted to in silico and in vitro evaluation of the antioxidant and cytotoxic properties of synthetic analogues of natural compounds—aromatic oligosulfides. The antiradical and SOD-protective activity of oligosulfides was demonstrated in the reaction with O2–• generated in enzymatic and non-enzymatic systems. It was found that phenol-containing disulfides significantly reduced the accumulation level of hydroperoxides and secondary carbonyl thiobarbituric acid reactive substances, which are primary products of oleic acid peroxidation. The antioxidant efficiency of bis(3,5-di-tert-butyl-4-hydroxyphenyl) disulfide increased over time due to the synergistic action of the 2,6-di-tert-butylphenol fragment and the disulfide linker. The highest cytotoxicity on the A-...

Research paper thumbnail of Study of Antioxidant Activity of New Compounds with 1,3-Thiazin-2,6-Dione and Pyrrolidine Fragments

Doklady Chemistry, 2021

Antioxidant activity of new compounds with 1,3-diazin-2,6-dione and pyrrolidine fragments in comp... more Antioxidant activity of new compounds with 1,3-diazin-2,6-dione and pyrrolidine fragments in comparison with 2,6-di-tert-butyl-4-mercaptophenol has been studied in the model system of adrenaline autooxidation in alkaline medium in DPPH, NBT, and CUPRAC tests. In the series of studied compounds, a leader has been revealed, which shows moderate antiradical activity toward DPPH radical, prevents accumulation of toxic products of adrenaline quinoid oxidation, and exhibits higher efficiency as one-electron Cu2+ reducing reagent as compared with known antioxidants, trolox and 2,6-di-tert-butyl-4-mercaptophenol.

Research paper thumbnail of Evaluation of antioxidant properties of new functionally substituted aryl carbamates

AIP Conference Proceedings, 2022

The antioxidative activity of new carbamate derivatives has been compared by studying their influ... more The antioxidative activity of new carbamate derivatives has been compared by studying their influence on the generation of superoxide anion radical O-• in the enzyme/substrate system xanthine/xanthine oxidase, on adrenaline autoxidation in an alkaline medium and on the level of lipid peroxidation in bester liver. Investigated aryl carbamates showed the inhibition of the nitroblue tetrazolium reduction by O-• generated in the xanthine/xanthine oxidase system, the accumulation of adrenaline oxidation reaction products both in the absence and in the presence of tilapia liver cytosol and the level of carbonyl oxidation by-products, which can react with thiobarbituric acid. These results showed that the new functionally substituted aryl carbamates possess a potent protective effect both preventing the development of oxidative stress and inhibiting oxidative processes under conditions of the development of oxidative stress.

Research paper thumbnail of Porphyrins with Phenolic Fragments at the Periphery of the Macrocycle as Perspective Antioxidants, Cytoprotectors and Heavy Metal Scavengers

Chemistry of Heterocyclic Compounds, 2021

The past few decades witnessed intense research devoted to the antioxidant activity of porphyrins... more The past few decades witnessed intense research devoted to the antioxidant activity of porphyrins. We review herein recent results of the studies of a series of porphyrins with antioxidant phenol groups in meso positions and an in vitro and in vivo study of their protective effects on free radical-induced damage of biological substances. The antioxidant activity of para-hydroxy-substituted meso-tetraphenylporphines and their metal complexes are summarized. The radical scavenging activity of porphyrins in relation to toxic species, including the highly reactive oxygen species is considered. The protective effects of these porphyrins have been proved in model reactions as well as in vitro and in vivo studies. A special attention is addressed to the polyfunctional porphyrins capable of preventing the toxicity of heavy metals due to antioxidant as well as scavenging ability.

Research paper thumbnail of Antioxidant activity of some organosulfur compounds in vitro

Arabian Journal of Chemistry, 2021

The medicinal properties of many natural plant products are related with the antioxidant action o... more The medicinal properties of many natural plant products are related with the antioxidant action of their constituents-oligosulfides and polyphenols. To increase the effectiveness of the antioxidant effect in lipid peroxidation and for reducing of undesirable toxic effects the combination in one molecule of several functional groups that act by various mechanisms seems one profitable. The goal of present study was to determine the antioxidant properties of synthetic sterically hindered 2,6-di-tert-butyl-4-mercaptophenol (1), bis(3,5-di-tert-butyl-4-hydroxyphenyl)disulfide (2) in comparison with disulfides containing in plant products: diphenyl disulfide (3), dibenzyl disulfide (4), dibutyl disulfide (5), di-tert-butyl disulfide (6), diallyl disulfide (7), methyl propyl trisulfide (8). Antioxidant properties were investigated by various assays including DPPH radical scavenging, Cu 2+ ion reduction (CUPRAC-test), model reactions of peroxidation of cis-9-octadecenoic (oleic) acid and lipids from the liver of Russian sturgeon in vitro as well as metal chelating activity. It was found that bis(3,5-di-tert-butyl-4-hydroxyphenyl) disulfide demonstrated both high DPPH radical scavenging activity and TEAC CUPRAC value and significantly decreased the level of primary products of lipid peroxidation (LPO)-lipid hydroperoxides (LOOH) as well as secondary productscarbonyl compounds which form colored complexes with thiobarbituric acid (TBARS). All sulfur compounds demonstrated the inhibitory activity in oleic acid peroxidation except dibutyl disulfide. The maximum decrease of LOOH and TBARS in lipid peroxidation was detected for bis(3,5-di-tertbutyl-4-hydroxyphenyl) disulfide. In the case of long-term LPO of liver lipids the antioxidant activity of all sulfur-containing compounds except diphenyl disulfide that exhibit pro-oxidant activity was ascertained. The efficiency of the antioxidant action of most compounds was decreased with time. Compound 2 was the only exception for which the increase in the efficiency of antioxidant

Research paper thumbnail of Synthesis and antioxidant activity of new hydroxy derivatives of chalcones

Russian Chemical Bulletin, 2020

New hydroxy derivatives of chalcones obtained via a condensation of 3-acetyl-2H-chromen-2-one wit... more New hydroxy derivatives of chalcones obtained via a condensation of 3-acetyl-2H-chromen-2-one with 2,4-dihydroxy-and 4-hydroxy-benzaldehydes and via an aldol crotonic condensation of methyl N-(4-acetylphenyl)carbamate with 4-hydroxy-3,5-di(tert-butyl)benzaldehyde have been isolated and characterized. A wide range of promising biological activity of the synthesized compounds, including the radical scavenging and antioxidant ones, was predicted by the in silico method. The anti-and pro-oxidant activities in model systems of peroxidation of cis-9-octadecenoic (oleic) acid and lipids from the liver homogenate of tilapia were estimated in vitro. The presence of sterically hindered hydroxy groups and a chromene moiety provides the prolonged antioxidant activity of new hydroxy derivatives of chalcones.

Research paper thumbnail of Cryoprotective activity of phosphorus-containing phenol

Cryobiology, 2020

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Research paper thumbnail of The Effect of Tin Compounds on the Lipid Peroxidation Level of Russian Sturgeon Fresh and Cryopreserved Sperm

Environmental Research, Engineering and Management, 2020

The effect of organotin compounds (OTs) on the accumulation of the lipid peroxidation (LPO) carbo... more The effect of organotin compounds (OTs) on the accumulation of the lipid peroxidation (LPO) carbonyl by-products, which react with thiobarbituric acid (TBARS) in fresh and cryopreserved sperm of Russian sturgeon, was studied. It was found that incubation (1 hour) of Russian sturgeon sperm with OTs (CH3SnCl3, (CH3)2SnCl2, (CH3)3SnCl, (n-C4H9)2SnCl2, (n-C4H9)3SnCl, (C6H5)2SnCl2, (C6H5)3SnCl) in concentration 0.1 mM led to the promotion of the accumulation of TBARS in native semen. Dimethyl- (DMT) and diphenyltin dichlorides (DPT) exhibited the greatest promoting activity, and the LPO level of both native and cryopreserved sperm of Russian sturgeon, including those in modified Stein's cryomedium, increased in the presence of these compounds. It was found that Russian sturgeon's cryopreserved sperm had lower sensitivity to the promotion of sperm LPO by DMT and DPT compared with the native sperm. The protective effect of Stein’s cryomedium decreased in the presence of the studied...

Research paper thumbnail of In silico and in vitro evaluation of the biological activity of some organicsulfur-containing compounds

TURKISH JOURNAL OF CHEMISTRY, 2019

It was predicted, using the Prediction of Activity Spectra for Substances online software, that o... more It was predicted, using the Prediction of Activity Spectra for Substances online software, that organic sulfur-containing compounds 2,6-di-tert-butyl-4-mercaptophenol (1), 2,6-di-tert-butyl-4-(3-mercaptopropyl)phenol (2), bis-3-[3,5-di-tert-butyl-4-hydroxyphenyl)propyl] sulfide (3), bis(3,5-di-tert-butyl-4-hydroxyphenyl) disulfide (4), diphenyl disulfide (5), dibenzyl disulfide (6), dibutyl disulfide (7), di-tert-butyl disulfide (8), diallyl disulfide (9), and methyl propyl trisulfide (10) could possess antioxidant activity and act as active scavengers of oxygen metabolites. The antiradical activity of these species was analyzed and it was shown that they were able to react with electrochemically generated the superoxide anion radical. The rate constants for reactions of 1-10 with the superoxide anion radical were calculated. The effect of the organic sulfur compounds on the rate of generation O −• 2 on adrenaline oxidation in an alkaline medium and on the ability of the biopreparation, based on the liver of Russian sturgeon, to deactivate O −• 2 was studied. The effect of the organic sulfur compounds on the rate of O −• 2 generation in the model system of the quinoid oxidation of adrenaline in an alkaline medium and on the ability of the biopreparation to deactivate O −• 2 was studied. It was found that O −• 2 generation rate decreased in the presence of the studied compounds, evidencing for their antiradical activity. Increase of superoxide dismutation activity of the biopreparation in the presence of compounds 1-10 was also shown, and this fact could indicate their ability to increase the antioxidant status and decrease the probability of oxidative stress development. It was found that compounds 1-10 did not inhibit enzyme lipoxygenase, which indicated the absence of antiinflammatory activity. The results of the forecast were consistent with the data of the experimental studies.

Research paper thumbnail of Application of new phenolic antioxidants for cryopreservation of sturgeon sperm

Cryobiology, 2016

Heterocyclic derivatives of butylated hydroxytoluene (BHT) were studied as cryoprotectants of the... more Heterocyclic derivatives of butylated hydroxytoluene (BHT) were studied as cryoprotectants of the basic media for cryopreservation of the Russian sturgeon sperm. Rates of lipid peroxidation of sturgeon sperm before and after cryopreservation were reduced in the presence of the studied compounds, exceeding the effects of BHT and water-soluble analogue of vitamin E, trolox. The most efficient antioxidant has the effective concentration of 0.1 mM. Novel antioxidant agents as cryomedium supplements not only reduced the level of lipid peroxidation, but also enhanced the translational motility of the sperm of the Russian sturgeon after defrosting.

Research paper thumbnail of Protecting Effect of the Free Base Porphyrins on the Rate of Hydrogen Peroxide Decomposition by Hemolysate of Human Blood Erythrocytes in the Presence of Mercury and Tin Compounds

Macroheterocycles, 2011

An influence of meso-tetraphenylporphyrin (TPPH 2) and its derivatives, i.e. meso-tetrakis(3,5-di... more An influence of meso-tetraphenylporphyrin (TPPH 2) and its derivatives, i.e. meso-tetrakis(3,5-di-tert-butyl-4hydroxyphenyl)porphyrin (R 4 PH 2), meso-tetra(4-sulfonatophenyl)porphine ((HO 3 S) 4 TPPН 2), in the presence of mercury and tin compounds on the rate of hydrogen peroxide decomposition by hemolysate of laundered human blood erythrocytes was studied. The decrease of the decomposition rate of hydrogen peroxide by erythrocytes in the presence of TPPH 2 and mercury (HgCl 2 , CH 3 HgI, Hg(NO 3) 2 •H 2 O) or tin ((CH 3) 2 SnCl 2 , (n-C 4 H 9) 3 SnCl, (n-C 4 H 9) 2 SnCl 2 , (C 6 H 5) 3 SnCl, (C 6 H 5) 2 SnCl 2)) compounds was ascertained. (n-C 4 H 9) 3 SnCl, which is used as biocidal additive in paints, is the most toxic in this row; the addition of this compound decreases the rate of H 2 O 2 decomposition by 70%. The influence of organotin compounds on the reaction rate depends on the nature of organic group and, in the case of butyl and methyl derivatives, on the quantity of these groups. The influence of addition of the extremely toxic methylmercury salts on the decomposition rate of hydrogen peroxide does not differ from the influence of inorganic mercury salts. The decrease of the H 2 O 2 decomposition rate in the presence of tin and mercury compounds may be connected with inhibition of catalase enzyme. At these conditions, an excess of hydrogen peroxide is formed, what brings to the change of permeability of biomembranes and oxidative stress. When (HO 3 S) 4 TPPН 2 is added in erythrocytes hemolysate the rate of enzymatic reaction is similar to control one. So an inclusion of sulfo group on the periphery of porphyrin molecule TPPH 2 eliminates the decrease of H 2 O 2 decomposition rate. The UV-vis spectrum of (HO 3 S) 4 TPPН 2 indicates the broadening and a small hypsochromic shift of the Soret band (5 nm) if compared with that of TPPH 2. Absorption intensity at the Q-band region (500-600 nm) is also decreased. An addition of R 4 PH 2 to erythrocytes hemolysate, containing tin compounds, causes an increase of catalase activity, thus the decrease of H 2 O 2 decomposition rate by toxicants is eliminated. Maximum efficiency of R 4 PH 2 additives was established in the presence of (n-C 4 H 9) 2 SnCl 2. An addition of TPPH 2 leads to the further reduce of the rate of H 2 O 2 decomposition by erythrocytes hemolysate in the presence of (CH 3) 3 SnCl and (n-C 4 H 9) 3 SnCl. The additives of sulfoporphyrin in hemolysate, containing CH 3 HgI, (CH 3) 3 SnCl, Hg(NO 3) 2 , HgCl 2 , results in increasing of H 2 O 2 decomposition rate. Water-soluble (HO 3 S) 4 TPPН 2 decreases the negative impact of tin and mercury compounds to the rate of enzymatic reactions. An increase of the rate of hydrogen peroxide decomposition by hemolysate of the laundered human blood erythrocytes, caused by R 4 PH 2 in the presence of organotin derivatives, contributes to the antioxidant status of blood erythrocytes in the conditions of the toxicants action.

Research paper thumbnail of Cryoprotective effect of phosphorous-containing phenolic anti-oxidant for the cryopreservation of beluga sperm

Cryobiology, 2014

A cryoprotective effect of an addition of a new synthetic antioxidant - a representative of phosp... more A cryoprotective effect of an addition of a new synthetic antioxidant - a representative of phosphorus-containing sterically hindered phenols is presented. The efficiency of the compound was shown to exceed the effect of lipid-soluble antioxidants butylated hydroxytoluene (BHT) and trolox in the conditions of cryopreservation of beluga sperm in the presence of the modified Stein's medium. It was shown that the level of carbonyl oxidation by-products, which can react with thiobarbituric acid (TBARS), in beluga sperm was inversely proportional to the motility time of sperm cells. The fertility of beluga sperm increased 2 times upon the addition of phosphorus-containing phenol to a modified Stein's medium. The prospects of the new antioxidant application to improve cryoresistance of beluga sperm in the conditions of cryopreservation for its efficient protection from the peroxidation processes are discussed.

Research paper thumbnail of Redox properties of novel pyrrolidine derivatives containing sterically hindered phenol fragment

Russian Journal of Electrochemistry, 2011

ABSTRACT The method of cyclic voltammetry on a platinum electrode in acetonitrile is used to stud... more ABSTRACT The method of cyclic voltammetry on a platinum electrode in acetonitrile is used to study the redox properties of a number of novel pyrrolidine derivatives containing a sterically hindered phenol fragment. It is found that the obtained derivatives of (3,5-di-tert-butyl-4-hydroxyphenyl)pyrrolidine-2-carboxylic acid are irreversibly oxidized in two stages with formation of a phenoxy radical. The structural analogue containing no phenyl fragment is irreversibly oxidized under similar conditions in a single stage. Formation of a stable phenoxy radical from synthesized (3,5-di-tert-butyl-4-hydroxyphenyl)pyrrolidines under PbO2 oxidation is confirmed by ESR spectroscopy. Application of the synthesized compounds as antioxidant agents has been suggested.

Research paper thumbnail of Radical scavenging activity of sterically hindered catecholate and o-amidophenolate complexes of LSbVPh3 type

Journal of Organometallic Chemistry, 2011

The radical scavenging effect of the substituted catecholates (1–3, 6) and o-amidophenolates (4, ... more The radical scavenging effect of the substituted catecholates (1–3, 6) and o-amidophenolates (4, 5) of triphenylantimony(V) in reactions with DPPH• radical and in a process of oleic acid peroxidation was studied in details. Complexes 1–6 show the high activity in radical scavenging reactions with DPPH• radical leading to disappearance of radical species. Complexes were demonstrated to be high-efficient inhibitors of

Research paper thumbnail of Antioxidant Activity and Toxic Effects of Phosphorus-Containing Derivatives of 2,6-Di-tert-Butylphenol in silico and in vitro, in vivo

Environmental Research, Engineering and Management, 2019

By using PASS online web resource, it was predicted that phosphorus-containing derivatives of 2,6... more By using PASS online web resource, it was predicted that phosphorus-containing derivatives of 2,6-di-tert-butylphenol had high probability to possess antioxidant activity and to act as active oxygen metabolite "traps" and heavy metal antidotes. The values of the acute toxicity index of phosphorus-containing phenols for rats, aquatic organisms, and bioaccumulation factor, calculated by the GUSAR programme, indicate potentially low toxicity. The results of the forecast are comparable with the data of experimental studies in vitro and in vivo experiments.

Research paper thumbnail of Synthesis and antioxidant activity of new hydroxy derivatives of chalcones

New hydroxy derivatives of chalcones obtained via a condensation of 3-acetyl-2H-chromen-2-one wit... more New hydroxy derivatives of chalcones obtained via a condensation of 3-acetyl-2H-chromen-2-one with 2,4-dihydroxy-and 4-hydroxy-benzaldehydes and via an aldol crotonic condensation of methyl N-(4-acetylphenyl)carbamate with 4-hydroxy-3,5-di(tert-butyl)benzaldehyde have been isolated and characterized. A wide range of promising biological activity of the synthesized compounds, including the radical scavenging and antioxidant ones, was predicted by the in silico method. The anti-and pro-oxidant activities in model systems of peroxidation of cis-9-octadecenoic (oleic) acid and lipids from the liver homogenate of tilapia were estimated in vitro. The presence of sterically hindered hydroxy groups and a chromene moiety provides the prolonged antioxidant activity of new hydroxy derivatives of chalcones.

Research paper thumbnail of The Effect of Tin Compounds on the Lipid Peroxidation Level of Russian Sturgeon Fresh and Cryopreserved Sperm The Effect of Tin Compounds on the Lipid Peroxidation Level of Russian Sturgeon Fresh and Cryopreserved Sperm

The effect of organotin compounds (OTs) on the accumulation of the lipid peroxidation (LPO) carbo... more The effect of organotin compounds (OTs) on the accumulation of the lipid peroxidation (LPO) carbonyl by-products , which react with thiobarbituric acid (TBARS) in fresh and cryopreserved sperm of Russian sturgeon, was studied. It was found that incubation (1 hour) of Russian sturgeon sperm with OTs (CH 3 SnCl 3 , (CH 3) 2 SnCl 2 , (CH 3) 3 SnCl, (n-C 4 H 9) 2 SnCl 2 , (n-C 4 H 9) 3 SnCl, (C 6 H 5) 2 SnCl 2 , (C 6 H 5) 3 SnCl) in concentration 0.1 mM led to the promotion of the accumulation of TBARS in native semen. Dimethyl-(DMT) and diphenyltin dichlorides (DPT) exhibited the greatest promoting activity, and the LPO level of both native and cryopreserved sperm of Russian sturgeon, including those in modified Stein's cryomedium, increased in the presence of these compounds. It was found that Russian sturgeon's cryopreserved sperm had lower sensitivity to the promotion of sperm LPO by DMT and DPT compared with the native sperm. The protective effect of Stein's cryomedium decreased in the presence of the studied OTs. The results suggest that accumulation of OTs by gonad of fish is another stress factor affecting the cell productivity in the cryopreservation process.