Kishalaya Das | Sambalpur University, (original) (raw)

Papers by Kishalaya Das

Research paper thumbnail of Is NESTROFT sufficient for mass screening for <i>β</i>-thalassaemia trait?

Journal of Medical Screening, Dec 1, 2007

Prevention of b-thalassaemia trait will, for the foreseeable future, hinge on effective screening... more Prevention of b-thalassaemia trait will, for the foreseeable future, hinge on effective screening strategies. Routine use of haematological data from automated cell counters may complement the results of the Naked Eye Single Tube Red cell Osmotic Fragility Test (NESTROFT), especially because of the high cost of a false-negative error. Our objective was to assess the potential additive value of routine haematological data in screening for b-thalassaemia trait. Settings Community survey of asymptomatic volunteers. Methods Using the NESTROFT results, haematological data and haemoglobin A 2 concentration from 1435 young, asymptomatic Sindhi subjects recruited in a population-based survey, and statistical analysis by classification tree approach, we examined whether haematological parameters have discriminatory utility additional to that of NESTROFT in screening for b-thalassaemia trait. Results We observed that in the derivation subset from which the classification tree was generated, there was only a marginal-albeit statistically significant-improvement in the screening performance of NESTROFT, whereas there was no such improvement attributable to the use of haematological parameters in a separate validation subset. Conclusion Our results further substantiate the claim that the use of NESTROFT is highly indicated for screening for b-thalassaemia trait in regions where the prevalence is high and the resources are constrained.

Research paper thumbnail of Effect of α+ Thalassemia on the Severity of Plasmodium falciparum Malaria in Different Sickle Cell Genotypes in Indian Adults: A Hospital-Based Study

Research paper thumbnail of Clinical Spectrum of Severe Plasmodium falciparum Malaria in a Tertiary Care Centre of Eastern India

International Journal of TROPICAL DISEASE & Health, 2016

Introduction: Plasmodium falciparum malaria is one of the major public health problem presented w... more Introduction: Plasmodium falciparum malaria is one of the major public health problem presented with varied clinical severity. This study was carried out to observe the clinical spectrum of severe falciparum malaria in a tertiary health care centre. Methods: This study was undertaken in hospitalized adults with suspected severe malaria. Confirmation of falciparum infection was done by ICT/QBC and single-step-PCR. Diagnosis of severe malaria was done by WHOguideline 2010. Original Research Article Purohit et al.; IJTDH, 12(4): 1-11, 2016; Article no.IJTDH.22387 2 Results: 450 adult cases with falciparum infection were studied. Maximum number of cases was from 15-25 years age group depicting the high exposure to malaria. In these patients, acute renal failure (ARF) was the most common (36.2%) complication followed by cerebral malaria (35.3%), jaundice (27.8%), hepatic dysfunction (21.8%), respiratory distress (18.4%), severe malarial anemia (15.8%), thrombocytopenia (15.1%), and hypoglycemia (9.3%). Mortality was found in 6.0% of cases. Cerebral involvement and ARF were the common cause of death in these patients. Conclusion: ARF is the most common type of clinical severity followed by cerebral malaria in adults and both are equally responsible for death along with other complications. Looking into the matter of varied clinical severity, accurate diagnosis, effective anti-malarial treatment along with supportive therapy is necessary to triumph over this deadly severe falciparum malaria.

Research paper thumbnail of Population structure and beta Thalassaemia among the Sindhis of Nagpur city

Research paper thumbnail of A1A2BO Rh (D) Blood groups haemoglobinopathies among Neo-Buddhist (Mahar) of Nagpur city

Research paper thumbnail of High prevalence of HbS and other abnormal haemoglbins in western Orissa: a temporal trend (patient populations)

Research paper thumbnail of Highest frequency of beta Thalassaemia trait among a Sindhi subgroup of Nagpur city: the Sakhrus

Research paper thumbnail of Distribution of some Genetic Polymorphism among the tribes of India

Journal of the Indian Anthropological Society / the Indian Anthropological Society

ABSTRACT

Research paper thumbnail of Abnormal haemoglobin (HbQ and HbD) and beta Thalassaemia among the Sindhis of Nagpur city – a brief report

Research paper thumbnail of Distribution of some genetic polymorphisms among the Indian tribes

Research paper thumbnail of Beta Thalassaemia among the Sindhis of Nagpur city – an evaluation of the awareness status

Research paper thumbnail of High frequency of haemoglobin D trait in Central India

Research paper thumbnail of Distribution of HbD in India – with special reference to the Sindhis

Research paper thumbnail of Sickle Cell Hemoglobin, Beta Thalassaemia and G-6-PD Deficiency in four tribes of the Sahyadris

Research paper thumbnail of Genetic differentiation among the Sindhis of Nagpur city with reference to beta Thalassaemia

Research paper thumbnail of Genetic couselling in tribals in India

The Indian Journal of Medical Research

ABSTRACT

Research paper thumbnail of Association of plasma homocysteine level with vaso-occlusive crisis in sickle cell anemia patients of Odisha, India

Annals of Hematology, 2019

Vascular complications of sickle cell anemia (SCA) are influenced by many factors. Elevated plasm... more Vascular complications of sickle cell anemia (SCA) are influenced by many factors. Elevated plasma homocysteine (Hcy) is supposed to be an independent risk factor and is either genetic or nutritional origin. The present study evaluated the plasma Hcy level, MTHFR C677T gene polymorphism, effect of folic acid (FA) supplementation‚ and hematobiochemical parameters in SCA and their effect on the vaso-occlusive crisis (VOC) in SCA patients of an Asian-Indian haplotype population. One hundred twenty cases of SCA (HbSS) and 50 controls with normal hemoglobin(HbAA) were studied. It was found that the plasma Hcy level is significantly higher (p < 0.0001) in patients with SCA (22.41 ± 7.8 μmol/L) compared to controls (13.2 ± 4.4 μmol/L). Moreover, patients without FA supplementation had a significantly (p < 0.001) higher Hcy level (27 ± 7 μmol/L) compared to those with supplementation (17.75 ± 5.7 μmol/L). Turkey-Kramer multiple comparison tests show that there is a significant difference (p < 0.05) in HbF percent, hemoglobin (Hb), platelet count, serum bilirubin (direct:Bil-D and total:Bil-T), aspartate transaminase (AST), lactate dehydrogenase (LDH), and plasma Hcy levels between mild and severe VOC. Between moderate VOC and severe VOC, there was a significant difference (p < 0.05) in HbF%, Bil-D, AST, Hcy. Pearson correlation revealed that plasma Hcy had a significantly (p < 0.05) positive correlation with AST, serum bilirubin (indirect and total), LDH, jaundice, stroke, VOC per year, and hospitalization per year whereas it was inversely correlated with HbF percentage, Hb level, and FA treatment. In the study population, increased plasma Hcy level, hemolysis, and platelet activation were found to influence VOC in SCA.

Research paper thumbnail of Beta Thalassaemia in Nagpur city: A proposed protocol for preventive measure

Research paper thumbnail of Distribution of HbS among the tribal population of central India

Research paper thumbnail of Association between XmnI Polymorphism (rs7482144) and HbF in Sickle Cell Disease: Evidence from a Meta-Analysis

Background: HbF has protective effect against painful crisis, acute chest syndrome, blood transfu... more Background: HbF has protective effect against painful crisis, acute chest syndrome, blood transfusion in Sickle Cell Disease patients. Many factors are associated for increase of HbF. Single nucleotide polymorphisms (SNPs) XmnI -158C>T Gγ-gene may participate in production of HbF, but the listed result are inconsistent and inconclusive. In present study, we performed a meta-analysis to summarize systemically the possible association of XmnI with HbF. Methodology: A total of 100HbSS, 45HbAS and 27HbAA cases ware confirmed, CE-HPLC was done for quantify various Hb fractions, XmnI polymorphism was studied by PCR-RFLP. A meta-analysis was performed to assess the possible role of XmnI polymorphism on the level of HbF. Hardy-Weinberg equilibrium (HWE), heterogeneity analysis; Egger’s linear regression test, forest plot and Begg’s funnel plot were performed. Results: Our finding shows significance relationship (p<0.05) between XmnI with HbF. A total of 225 cases and 96 controls from ...

Research paper thumbnail of Is NESTROFT sufficient for mass screening for <i>β</i>-thalassaemia trait?

Journal of Medical Screening, Dec 1, 2007

Prevention of b-thalassaemia trait will, for the foreseeable future, hinge on effective screening... more Prevention of b-thalassaemia trait will, for the foreseeable future, hinge on effective screening strategies. Routine use of haematological data from automated cell counters may complement the results of the Naked Eye Single Tube Red cell Osmotic Fragility Test (NESTROFT), especially because of the high cost of a false-negative error. Our objective was to assess the potential additive value of routine haematological data in screening for b-thalassaemia trait. Settings Community survey of asymptomatic volunteers. Methods Using the NESTROFT results, haematological data and haemoglobin A 2 concentration from 1435 young, asymptomatic Sindhi subjects recruited in a population-based survey, and statistical analysis by classification tree approach, we examined whether haematological parameters have discriminatory utility additional to that of NESTROFT in screening for b-thalassaemia trait. Results We observed that in the derivation subset from which the classification tree was generated, there was only a marginal-albeit statistically significant-improvement in the screening performance of NESTROFT, whereas there was no such improvement attributable to the use of haematological parameters in a separate validation subset. Conclusion Our results further substantiate the claim that the use of NESTROFT is highly indicated for screening for b-thalassaemia trait in regions where the prevalence is high and the resources are constrained.

Research paper thumbnail of Effect of α+ Thalassemia on the Severity of Plasmodium falciparum Malaria in Different Sickle Cell Genotypes in Indian Adults: A Hospital-Based Study

Research paper thumbnail of Clinical Spectrum of Severe Plasmodium falciparum Malaria in a Tertiary Care Centre of Eastern India

International Journal of TROPICAL DISEASE & Health, 2016

Introduction: Plasmodium falciparum malaria is one of the major public health problem presented w... more Introduction: Plasmodium falciparum malaria is one of the major public health problem presented with varied clinical severity. This study was carried out to observe the clinical spectrum of severe falciparum malaria in a tertiary health care centre. Methods: This study was undertaken in hospitalized adults with suspected severe malaria. Confirmation of falciparum infection was done by ICT/QBC and single-step-PCR. Diagnosis of severe malaria was done by WHOguideline 2010. Original Research Article Purohit et al.; IJTDH, 12(4): 1-11, 2016; Article no.IJTDH.22387 2 Results: 450 adult cases with falciparum infection were studied. Maximum number of cases was from 15-25 years age group depicting the high exposure to malaria. In these patients, acute renal failure (ARF) was the most common (36.2%) complication followed by cerebral malaria (35.3%), jaundice (27.8%), hepatic dysfunction (21.8%), respiratory distress (18.4%), severe malarial anemia (15.8%), thrombocytopenia (15.1%), and hypoglycemia (9.3%). Mortality was found in 6.0% of cases. Cerebral involvement and ARF were the common cause of death in these patients. Conclusion: ARF is the most common type of clinical severity followed by cerebral malaria in adults and both are equally responsible for death along with other complications. Looking into the matter of varied clinical severity, accurate diagnosis, effective anti-malarial treatment along with supportive therapy is necessary to triumph over this deadly severe falciparum malaria.

Research paper thumbnail of Population structure and beta Thalassaemia among the Sindhis of Nagpur city

Research paper thumbnail of A1A2BO Rh (D) Blood groups haemoglobinopathies among Neo-Buddhist (Mahar) of Nagpur city

Research paper thumbnail of High prevalence of HbS and other abnormal haemoglbins in western Orissa: a temporal trend (patient populations)

Research paper thumbnail of Highest frequency of beta Thalassaemia trait among a Sindhi subgroup of Nagpur city: the Sakhrus

Research paper thumbnail of Distribution of some Genetic Polymorphism among the tribes of India

Journal of the Indian Anthropological Society / the Indian Anthropological Society

ABSTRACT

Research paper thumbnail of Abnormal haemoglobin (HbQ and HbD) and beta Thalassaemia among the Sindhis of Nagpur city – a brief report

Research paper thumbnail of Distribution of some genetic polymorphisms among the Indian tribes

Research paper thumbnail of Beta Thalassaemia among the Sindhis of Nagpur city – an evaluation of the awareness status

Research paper thumbnail of High frequency of haemoglobin D trait in Central India

Research paper thumbnail of Distribution of HbD in India – with special reference to the Sindhis

Research paper thumbnail of Sickle Cell Hemoglobin, Beta Thalassaemia and G-6-PD Deficiency in four tribes of the Sahyadris

Research paper thumbnail of Genetic differentiation among the Sindhis of Nagpur city with reference to beta Thalassaemia

Research paper thumbnail of Genetic couselling in tribals in India

The Indian Journal of Medical Research

ABSTRACT

Research paper thumbnail of Association of plasma homocysteine level with vaso-occlusive crisis in sickle cell anemia patients of Odisha, India

Annals of Hematology, 2019

Vascular complications of sickle cell anemia (SCA) are influenced by many factors. Elevated plasm... more Vascular complications of sickle cell anemia (SCA) are influenced by many factors. Elevated plasma homocysteine (Hcy) is supposed to be an independent risk factor and is either genetic or nutritional origin. The present study evaluated the plasma Hcy level, MTHFR C677T gene polymorphism, effect of folic acid (FA) supplementation‚ and hematobiochemical parameters in SCA and their effect on the vaso-occlusive crisis (VOC) in SCA patients of an Asian-Indian haplotype population. One hundred twenty cases of SCA (HbSS) and 50 controls with normal hemoglobin(HbAA) were studied. It was found that the plasma Hcy level is significantly higher (p < 0.0001) in patients with SCA (22.41 ± 7.8 μmol/L) compared to controls (13.2 ± 4.4 μmol/L). Moreover, patients without FA supplementation had a significantly (p < 0.001) higher Hcy level (27 ± 7 μmol/L) compared to those with supplementation (17.75 ± 5.7 μmol/L). Turkey-Kramer multiple comparison tests show that there is a significant difference (p < 0.05) in HbF percent, hemoglobin (Hb), platelet count, serum bilirubin (direct:Bil-D and total:Bil-T), aspartate transaminase (AST), lactate dehydrogenase (LDH), and plasma Hcy levels between mild and severe VOC. Between moderate VOC and severe VOC, there was a significant difference (p < 0.05) in HbF%, Bil-D, AST, Hcy. Pearson correlation revealed that plasma Hcy had a significantly (p < 0.05) positive correlation with AST, serum bilirubin (indirect and total), LDH, jaundice, stroke, VOC per year, and hospitalization per year whereas it was inversely correlated with HbF percentage, Hb level, and FA treatment. In the study population, increased plasma Hcy level, hemolysis, and platelet activation were found to influence VOC in SCA.

Research paper thumbnail of Beta Thalassaemia in Nagpur city: A proposed protocol for preventive measure

Research paper thumbnail of Distribution of HbS among the tribal population of central India

Research paper thumbnail of Association between XmnI Polymorphism (rs7482144) and HbF in Sickle Cell Disease: Evidence from a Meta-Analysis

Background: HbF has protective effect against painful crisis, acute chest syndrome, blood transfu... more Background: HbF has protective effect against painful crisis, acute chest syndrome, blood transfusion in Sickle Cell Disease patients. Many factors are associated for increase of HbF. Single nucleotide polymorphisms (SNPs) XmnI -158C>T Gγ-gene may participate in production of HbF, but the listed result are inconsistent and inconclusive. In present study, we performed a meta-analysis to summarize systemically the possible association of XmnI with HbF. Methodology: A total of 100HbSS, 45HbAS and 27HbAA cases ware confirmed, CE-HPLC was done for quantify various Hb fractions, XmnI polymorphism was studied by PCR-RFLP. A meta-analysis was performed to assess the possible role of XmnI polymorphism on the level of HbF. Hardy-Weinberg equilibrium (HWE), heterogeneity analysis; Egger’s linear regression test, forest plot and Begg’s funnel plot were performed. Results: Our finding shows significance relationship (p<0.05) between XmnI with HbF. A total of 225 cases and 96 controls from ...