Anil Sirohi | Sardar Vallabh Bhai Patel University Of Agriculture and Technology (original) (raw)

Papers by Anil Sirohi

Progressive …, 2009

... INTER-SSR AND SSR BASED MOLECULAR PROFILING OF BASMATI AND NON-BASMATI INDICA RICE D.Singh, A... more ... INTER-SSR AND SSR BASED MOLECULAR PROFILING OF BASMATI AND NON-BASMATI INDICA RICE D.Singh, A. Kumar, A. Sirohi, DR Purohit1 , HS Dhaliwal1 , V. Kumar and S. Chand ... 23. Sathi-Safed Chhatisgarh Semi dwarf, short duration, incomplete exertion 24. ...

Indian Journal of Agricultural Sciences, Dec 16, 2016

A study was conducted for estimating genetic parameters involving 10 parents and their 45 F 1 s (... more A study was conducted for estimating genetic parameters involving 10 parents and their 45 F 1 s (half diallel) of bread wheat (Triticum aestivum L.) der timely sown condition of north western plains zone of India during 2011-12 and 2012-13. Positive and significant values of F component were estimated for yield and its contributing traits in F 1 s indicating the preponderance of dominance and positive genes in the parents involed. The estimates of h 2 were positive and significant for days to maturity (2.97), plant height (28.14), peduncle length (5.48), productive tillers (0.55), spike length (0.76), spikelets/spike (26.28), grains/spike (228.45), biological yield (149.71), grain yield (73.58), harvest index (33.15), and 1 000 grain weight (21.71). The theoretical value (0.25) of (H 2 /4H 1) for all the traits indicated asymmetrical distribution of positive and negative genes. The proportion of dominant and recessive alleles for days to 50% flowering, days to maturity, plant height, peduncle length, productive tillers, spike length, biological yield, grain yield, harvest index and 1 000 grain weight, indicated preponderance of dominant alleles in the parents for these traits, whereas traits namely; flag leaf area, spikelets/spikes, grains/spike and gluten content, reflected more recessive alleles in the parents. Estimates of SCA effects revealed that the best cross combinations for respective traits were K 9162 ×

Biotechnology journal international, May 18, 2020

Plants during their growth, experience periodic stress conditions both abiotic (adverse environme... more Plants during their growth, experience periodic stress conditions both abiotic (adverse environmental conditions) as well as biotic (infection by pathogens). They appear to respond to these adverse conditions by modulating the expression of many genes. One of the pronounced effects of stress on plant is the enhanced synthesis of a set of proteins-termed ' stress proteins'. Lentil contains asset of genes/proteins which helps this crop to overcome abiotic stresses. In the present study, HSP70 (Heat Shock Protein), LEA (Late Embryogenesis Abundant) and Aldolase genes were identified and cloned in pTZ57RT vector followed by sequencing. Expression analysis was done through Q-PCR which was assessed by using cDNA from all the heat, drought and salinity stressed and unstressed lentil cotyledons. The highest level of transcript of HSP70 was realized upon exposure to heat at 45°C for 3 hour followed by at 45°C for 2 hour and lowest at 40°C for 1hour. LEA gene was identified under drought and salinity stress and highest transcript was at 20% PEG for 3 hour (drought stress) and in salinity stress highest transcript was at 150 mm for 6 hour. For Aldolase gene highest transcript was recorded after 3, 6 and 12 hr at 100 mM, 150

Progressive Agriculture, 2010

Environment and Ecology, 2012

Progressive Agriculture, 2009

Ten diverse genotypes of green gram (Vigna radiata L. Wilczek) were evaluated for yield and nine ... more Ten diverse genotypes of green gram (Vigna radiata L. Wilczek) were evaluated for yield and nine other economic traits during the kharif season of 2006 at Crop Research Centre of Sardar Vallabh Bhai Patel University of Agriculture & Technology, Meerut. The study revealed considerable genetic variability among ten genotypes for days to 50% flowering, days to 75% maturity, plant height (em), number of productive branches per plant, number of productive pods per plant, number of seeds per pod, 100-seed weight (g), biological yield per plant (g), harvest Index (%) and seed yield per plant (g). High heritability coupled with high expected genetic advance observed for harvest index, pods per plant, seed yield per plant, biological yield per plant, 100-seed weight, seed per pod, plant he1ght and number of productive branches per plant revealed the preponderance of additive gene effects in the expression of these traits. Seed yield per plant exhibited positive association with harvest index...

Annals of Plant Protection Sciences, 2010

Six treatments viz., BeaUveria bassiana @ 2.0 kg/ha., Metarrhizium anisopliae. @ 2.0 kg/ha, NSKE ... more Six treatments viz., BeaUveria bassiana @ 2.0 kg/ha., Metarrhizium anisopliae. @ 2.0 kg/ha, NSKE 5% neemarin @ 3 lit/ha., B.t. @ 0.5 kg/ha and cartap hydrochloride (4G) @ 15 kg/ha at sowing time + endosulfan (35EC) @ 1.5 lit/ha and an untreated control. The minimum stem fly infestation of 1.3 and 1.4% was recorded with cartap hydrochloride 4G + endosulfan 35EC during rab; 2006–2007 and rabi 2007–2008, respectively. The treatment with BI was found best with minimum pod borer infestation of 7.3 and 4.4% during rabi 2006–2007 and rabi 2007-200S, respectively. The maximum average yield of 17.54 q/ha, maximum net return of Rs 13830/ha and highest cost benefit ratio (7.1) was obtained with cartap hydrochloride 4G + endosulfan 35EC.

The present report communicates the first complete genome sequence of Brucella abortus 2308 strai... more The present report communicates the first complete genome sequence of Brucella abortus 2308 strain isolated from a an abortion storm in a dairy farm located at Kanpur, Uttar Pradesh in India. It caused the last trimester abortions of 32 animals out of 100 cows in a dairy over a period of 60 days. The bacteria were isolated in pure culture from the placenta of aborted cows. The genome sequence length of isolated bacteria is 3,285,606 bp with a 57.25 % GC content, an N50 value of 296,426, L50 value of 4 containing 3,119 coding DNA sequences (CDSs), 49 tRNAs, 1 transfer messenger RNA (mRNA), and 3 rRNA genes. It is the first report of Brucella abortus 2308 isolation and complete genome sequence from Indian subcontinent.

Genetic diversity among thirty two accessions of rice representing landraces, released varieties,... more Genetic diversity among thirty two accessions of rice representing landraces, released varieties, cultivars and breeding lines was analysed using simple sequence repeat (SSR), inter-simple sequence repeat (ISSR) and three dwarfing trait based markers. A total of 81 polymorphic bands were detected with 20 primer combinations. The genetic similarity among the accessions ranged from (0.60) to (0.97) with a mean of 0.737. All 32 accessions were clustered into 5 major groups. Of the 32 pair wise combinations generated by rice genotypes, the lowest coefficient of similarity 0.505 was observed between Sarbati-A and Sathi, Bagni and VB24 genotypes. The highest coefficient of similarity 0.964 was obtained between VD 62 and VLD86, which appear as the most similar and closely related accessions. Results illustrated the high-level polymorphism obtained by SSR and ISSR, especially trait-linked markers used in this study. These markers will applicable in discriminating different germplasm, and ut...

Microbiology Resource Announcements

The present report communicates the first complete genome sequence of Brucella abortus strain 230... more The present report communicates the first complete genome sequence of Brucella abortus strain 2308, isolated from an abortion storm on a dairy farm in India. Bacteria were isolated in pure culture from the placentas of aborted calves, and next-generation sequencing (NGS) revealed that the genome sequence length of the isolated strain is 3,285,606 bp, with a GC content of 57.25%, an N 50 value of 296,426 bp, and an L 50 value of 4, containing 3,119 coding DNA sequences (CDSs), 49 tRNAs, 1 transfer-messenger RNA (tmRNA), and 3 rRNA genes.

Progressive Agriculture, 2011

Legume Research, 2009

Correlation and path coefficient analysis were done with 53 chickpea genotypes over two seasonsto... more Correlation and path coefficient analysis were done with 53 chickpea genotypes over two seasonsto find out association among characters and to assess the direct and indirect contribution of ninecharacters on seed yield. The studies were conducted during rabi 2000-01 and rabi 2001-02. Correlationstudies indicated that seed yield per plant exhibited stable positive association with biological yieldper plant, pods per plant, primary branches per plant, plant height and harvest index at genotypicand phenotypic levels in individually as well as combined over seasons. Path analysis revealed highpositive and direct influence of biological yield per plant with seed yield per plant followed byharvest index and pods per plant in individual as well as combined over seasons. Pods per plant,primary branches per plant and plant height contributed to seed yield mainly through indirect effectvia biological yield. Therefore, selection for high biological yield and harvest index would lead tohigh see...

Rice genotypes were evaluated for resistance to the Brown Plant Hopper (BPH), Nilaparvata lugens ... more Rice genotypes were evaluated for resistance to the Brown Plant Hopper (BPH), Nilaparvata lugens Stal in SVPUA & T Meerut, were evaluated for resistance in greenhouse screening tests. Over a period of two years (2017 and 2018), 17 rice cultivars were screened using the plant standard seed box screening technique. The results showed 6 rice varieties viz. REMRE-1, RPMRE-2, RPMRE-3, RPMRE-5, RIL501 and PTB-33 were possessing high level of resistance to the BPH. 3 rice varieties viz., Vallabh Basmati-24, Pusa Sugandha-5, Taraori Basmati and Pusa Basmati-1 were moderately resistant. 4 rice varieties viz., Kasturi, Vallabh Basmati-23, Vallabh Basmati-22, Improved Pusa Basmati-1460 were moderately susceptible and 3 rice varieties viz., Pusa Basmati-6, Pusa Basmati-1121 and Pusa Basmati1509 were susceptible, according to standard evaluation system (IRRI, 1992) Standard Seed Box Screening technique (SSST).

Indian Journal of Plant Genetic Resources, 1979

A wide range of genetic divergence was noted in 25 genotypes of Chickpea for six yield related tr... more A wide range of genetic divergence was noted in 25 genotypes of Chickpea for six yield related traits. These strains were grouped into 7 dusters. The cuhivars within a duster showed smaller D2 values than those belonging to two different dusters. 100-seed weight and seed yield per plant were chiefly responsible for genetic divergence. Based on inter-duster distances, crossing of duster IV genotypes with V, VI and VII was suggested to get maximum heterotic effect in F1 and a broad spectrum of variability in the segregating generations to isolate superior individuals for yield and its components.

Journal of Pharmacognosy and Phytochemistry, 2017

Rice is the first cereal genome with a complete sequence and a model crop that has important rela... more Rice is the first cereal genome with a complete sequence and a model crop that has important relationships with other cereal species. It has a small genome size of 430 Mb (IRGSP, 2005) which is one-tenth the size of the human genome. The complete sequence is available for both japonica and Indica genomes. Among the thirty rice genotypes, PB-2 showed the minimum disease severity at 7 DAI (1.40%) and at 14 DAI (1.50%) followed by Tetep showed the minimum disease severity at 7 DAI (1.40%) and at 14 DAI (1.50%) from tillering stages whereas, Basmati-370 showed the maximum disease severity at 7 DAI (3.90%) and at 14 DAI (3.93%) from tillering stages. The five genotypes (Ranbeer Basmati, PB-1, VB-23, BPT-5204 and B-370) which showed the absence of Blast resistance genes indicated the highest AUDPC (65-84), therefore regarded as BLAST susceptible genotypes. The five genotypes (HB-1, N-22, PR-106, Tetep and PB-2) which showed the absence of Blast resistance genes indicated the lowest AUDPC ...

The genetic diversity among 24 chrysanthemum cultivars was investigated by 07 Simple Sequence Rep... more The genetic diversity among 24 chrysanthemum cultivars was investigated by 07 Simple Sequence Repeats (SSRs). A total of 16 bands were produced out of which 15 bands were found polymorphic and 01 band monomorphic. The number of polymorphic fragment varied from 02 (RM1) to 03 (RM433) with an average 2.14 fragment per primer and percent polymorphism varied from 66.75 to 100% with an average of 93.75%. The PIC varied from 0.42 to 0.95 with an average of 0.74. The RP and MI ranged from (0.83 to 0,57) to (4.0 and 2.76) with an average (2.03 and 0.57) respectively. The UPGMA clustering revealed two major groups and found considerable amount of genetic diversity. Among the 24 cultivars, Ravikiran, Selection 44, Kundan, Terri, Sonton and Poncho are divergent and may be used for breeding programme Results suggested that SSRs are highly useful for assessing the genetic diversity analysis among the chrysanthemum germplasm and parental selection studies in chrysanthemum.

Pulse Improvement, 2018

Pulses are an important source of protein for developing countries in Asia, Africa, and Latin Ame... more Pulses are an important source of protein for developing countries in Asia, Africa, and Latin America. The reasons for low consumption of pulses include the fact that pulses have been regarded as “protein for the poor,” a lack of familiarity with the different types and their benefits, limited attractiveness, long cooking time, and limited pulse production. On the basis of average annual data for the period 2011–2013, about 5.8% of the world’s arable land area is occupied by pulses, which are grown mainly on less fertile and marginal land or as intercrops with cereals and oilseeds. Pulses have importance in contributing to food and nutritional security and in replenishing soil nutrients, with a huge potential to address needs such as future global food security, nutrition, and environmental sustainability. Shortfalls in pulse availability are mainly due to a lower seed replacement rate for improved varieties, poor adoption of technologies by farmers, climate change, pests, disease, and declining total factor productivity. In addition, biotic and abiotic stresses, lack of access to quality inputs, and limited industrial development have hampered improvements in the productivity of pulses, and expansion of pulse cultivation has been neglected. The high demand for pulses must be met by an increase in yield through strengthening of adaptive research, technology assessment, and refinement and transfer capabilities, so that the existing technology transfer gaps can be bridged. Pulses represent important economic opportunities to increase the income of large and small farmers and to reduce risk by diversifying their crops and income stream portfolio. Increased investment in research and development to improve productivity, and to make the information produced accessible to and understandable by farmers, is greatly needed. There is also a need for increased investment in breeding underutilized, high-quality varieties that are pest, disease, and climate resilient.