Shilpa R | Vtu - Academia.edu (original) (raw)

Papers by Shilpa R

Research paper thumbnail of Structural and functional analyses of minimal phosphopeptides targeting the polo-box domain of polo-like kinase 1

Nature Structural & Molecular Biology, 2009

In the version of this article initially published, in several instances "guanidine" should have ... more In the version of this article initially published, in several instances "guanidine" should have read "guanine", "uridine" should have read "uracil", and "adenine" should have read adenosine; in two instances; "tetratrispolin" should have read "tristetraprolin"; and ,d should have illustrated the structure from PDB record 1M8Y. These errors have been corrected in the HTML and PDF versions of the article. e r r ata a n D co r r i g e n Da

Research paper thumbnail of Crystallographic, thermodynamic, and molecular modeling studies of the mode of binding of oligosaccharides to the potent antiviral protein griffithsin

Proteins-structure Function and Bioinformatics, 2007

The mode of binding of oligosaccharides to griffithsin, an antiviral lectin from the red alga Gri... more The mode of binding of oligosaccharides to griffithsin, an antiviral lectin from the red alga Griffithsia sp., was investigated by a combination of X-ray crystallography, isothermal titration calorimetry, and molecular modeling. The structures of complexes of griffithsin with 1?6a-mannobiose and with maltose were solved and refined at the resolution of 2.0 and 1.5 Å , respectively. The thermodynamic parameters of binding of 1?6amannobiose, maltose, and mannose to griffithsin were determined. Binding profiles of 1?6a-mannobiose and mannose were similar with K d values of 83.3 lM and 102 lM, respectively. The binding of maltose to griffithsin was significantly weaker, with a fourfold lower affinity (K d = 394 lM). In all cases the binding at 308C was entropically rather than enthalpically driven. On the basis of the experimental crystal structures, as well as on previously determined structures of complexes with monosaccharides, it was possible to create a model of a tridentate complex of griffithsin with Man 9 GlcNAc 2 , a high mannose oligosaccharide commonly found on the surface of viral glycoproteins. All shorter oligomannoses could be modeled only as bidentate or monodentate complexes with griffithsin. The ability to mediate tight multivalent and multisite interactions with high-mannose oligosaccharides helps to explain the potent antiviral activity of griffithsin. Proteins 2007;67:661-670. V V C 2007 Wiley-Liss, Inc.*

Research paper thumbnail of Crystallographic studies of the complexes of antiviral protein griffithsin with glucose and N-acetylglucosamine

Research paper thumbnail of Nutrient Deficiencies in the Premature Infant

Pediatric Clinics of North America, 2009

Premature infants are a population prone to nutrient deficiencies. Because the early diet of thes... more Premature infants are a population prone to nutrient deficiencies. Because the early diet of these infants is entirely amenable to intervention, understanding the pathophysiology behind these deficiencies is important for both the neonatologists who care for them acutely and for pediatricians who are responsible for their care through childhood. This article reviews the normal accretion of nutrients in the fetus, discusses specific nutrient deficiencies that are exacerbated in the postnatal period, and identifies key areas for future research.

Research paper thumbnail of Early Emphysematous Changes in Asymptomatic Smokers: Detection with 3He MR Imaging

Radiology, 2006

disparity between respiratory effort and the Vt response. Changes in dyspnea intensity after tiot... more disparity between respiratory effort and the Vt response. Changes in dyspnea intensity after tiotropium were positively correlated with changes in this index of neuromechanical coupling.

Research paper thumbnail of Detection of Age-Dependent Changes in Healthy Adult Lungs With Diffusion-Weighted 3He MRI

Academic Radiology, 2005

To investigate changes in lung microstructure in healthy adult subjects with no smoking history u... more To investigate changes in lung microstructure in healthy adult subjects with no smoking history using diffusion-weighted 3He MRI. Diffusion magnetic resonance imaging using hyperpolarized helium 3 (3He) was applied to healthy volunteers to explore the dependence of lung microstructural changes with age, reflected by changes in the apparent diffusion coefficient (ADC) of 3He in lung air spaces. Data from three sites (University of Virginia (UVa), N = 25; University of Wisconsin (UW), N = 8; University of Nottingham (UN), N = 11) were combined in pooled analysis, including a total of N = 44 subjects (age range, 18-69 years; average age, 41.7 +/- 16.7 years). ADC was found to depend on age at all three sites (UW, R = +0.95, P = .0003; UVa, R = +0.74, P < .0001; UN, R = +0.96, P < .0001). Increases in mean ADCs with age appeared similar across sites (UW, +0.0017 cm2 s(-1) y(-1); UVa, +0.0015 cm2 s(-1) y(-1); pooled, +0.0015 cm2 s(-1) y(-1); P = .71). In a regional analysis performed on UW data, the increase in ADC affected all regions of the lung, but the apical and middle regions showed a greater increase compared with the base of the lung. Results suggest the observed age dependence of the ADC may be caused by changes in lung microstructure that increase alveolar volume during the aging process.

Research paper thumbnail of Structural and functional analyses of minimal phosphopeptides targeting the polo-box domain of polo-like kinase 1

Nature Structural & Molecular Biology, 2009

In the version of this article initially published, in several instances "guanidine" should have ... more In the version of this article initially published, in several instances "guanidine" should have read "guanine", "uridine" should have read "uracil", and "adenine" should have read adenosine; in two instances; "tetratrispolin" should have read "tristetraprolin"; and ,d should have illustrated the structure from PDB record 1M8Y. These errors have been corrected in the HTML and PDF versions of the article. e r r ata a n D co r r i g e n Da

Research paper thumbnail of Crystallographic, thermodynamic, and molecular modeling studies of the mode of binding of oligosaccharides to the potent antiviral protein griffithsin

Proteins-structure Function and Bioinformatics, 2007

The mode of binding of oligosaccharides to griffithsin, an antiviral lectin from the red alga Gri... more The mode of binding of oligosaccharides to griffithsin, an antiviral lectin from the red alga Griffithsia sp., was investigated by a combination of X-ray crystallography, isothermal titration calorimetry, and molecular modeling. The structures of complexes of griffithsin with 1?6a-mannobiose and with maltose were solved and refined at the resolution of 2.0 and 1.5 Å , respectively. The thermodynamic parameters of binding of 1?6amannobiose, maltose, and mannose to griffithsin were determined. Binding profiles of 1?6a-mannobiose and mannose were similar with K d values of 83.3 lM and 102 lM, respectively. The binding of maltose to griffithsin was significantly weaker, with a fourfold lower affinity (K d = 394 lM). In all cases the binding at 308C was entropically rather than enthalpically driven. On the basis of the experimental crystal structures, as well as on previously determined structures of complexes with monosaccharides, it was possible to create a model of a tridentate complex of griffithsin with Man 9 GlcNAc 2 , a high mannose oligosaccharide commonly found on the surface of viral glycoproteins. All shorter oligomannoses could be modeled only as bidentate or monodentate complexes with griffithsin. The ability to mediate tight multivalent and multisite interactions with high-mannose oligosaccharides helps to explain the potent antiviral activity of griffithsin. Proteins 2007;67:661-670. V V C 2007 Wiley-Liss, Inc.*

Research paper thumbnail of Crystallographic studies of the complexes of antiviral protein griffithsin with glucose and N-acetylglucosamine

Research paper thumbnail of Nutrient Deficiencies in the Premature Infant

Pediatric Clinics of North America, 2009

Premature infants are a population prone to nutrient deficiencies. Because the early diet of thes... more Premature infants are a population prone to nutrient deficiencies. Because the early diet of these infants is entirely amenable to intervention, understanding the pathophysiology behind these deficiencies is important for both the neonatologists who care for them acutely and for pediatricians who are responsible for their care through childhood. This article reviews the normal accretion of nutrients in the fetus, discusses specific nutrient deficiencies that are exacerbated in the postnatal period, and identifies key areas for future research.

Research paper thumbnail of Early Emphysematous Changes in Asymptomatic Smokers: Detection with 3He MR Imaging

Radiology, 2006

disparity between respiratory effort and the Vt response. Changes in dyspnea intensity after tiot... more disparity between respiratory effort and the Vt response. Changes in dyspnea intensity after tiotropium were positively correlated with changes in this index of neuromechanical coupling.

Research paper thumbnail of Detection of Age-Dependent Changes in Healthy Adult Lungs With Diffusion-Weighted 3He MRI

Academic Radiology, 2005

To investigate changes in lung microstructure in healthy adult subjects with no smoking history u... more To investigate changes in lung microstructure in healthy adult subjects with no smoking history using diffusion-weighted 3He MRI. Diffusion magnetic resonance imaging using hyperpolarized helium 3 (3He) was applied to healthy volunteers to explore the dependence of lung microstructural changes with age, reflected by changes in the apparent diffusion coefficient (ADC) of 3He in lung air spaces. Data from three sites (University of Virginia (UVa), N = 25; University of Wisconsin (UW), N = 8; University of Nottingham (UN), N = 11) were combined in pooled analysis, including a total of N = 44 subjects (age range, 18-69 years; average age, 41.7 +/- 16.7 years). ADC was found to depend on age at all three sites (UW, R = +0.95, P = .0003; UVa, R = +0.74, P < .0001; UN, R = +0.96, P < .0001). Increases in mean ADCs with age appeared similar across sites (UW, +0.0017 cm2 s(-1) y(-1); UVa, +0.0015 cm2 s(-1) y(-1); pooled, +0.0015 cm2 s(-1) y(-1); P = .71). In a regional analysis performed on UW data, the increase in ADC affected all regions of the lung, but the apical and middle regions showed a greater increase compared with the base of the lung. Results suggest the observed age dependence of the ADC may be caused by changes in lung microstructure that increase alveolar volume during the aging process.