zhang han li | The University of Sydney (original) (raw)
Papers by zhang han li
Analytical and bioanalytical chemistry, 2014
Plasmid calibrators are increasingly applied for polymerase chain reaction (PCR) analysis of gene... more Plasmid calibrators are increasingly applied for polymerase chain reaction (PCR) analysis of genetically modified organisms (GMOs). To evaluate the commutability between plasmid DNA (pDNA) and genomic DNA (gDNA) as calibrators, a plasmid molecule, pBSTopas, was constructed, harboring a Topas 19/2 event-specific sequence and a partial sequence of the rapeseed reference gene CruA. Assays of the pDNA showed similar limits of detection (five copies for Topas 19/2 and CruA) and quantification (40 copies for Topas 19/2 and 20 for CruA) as those for the gDNA. Comparisons of plasmid and genomic standard curves indicated that the slopes, intercepts, and PCR efficiency for pBSTopas were significantly different from CRM Topas 19/2 gDNA for quantitative analysis of GMOs. Three correction methods were used to calibrate the quantitative analysis of control samples using pDNA as calibrators: model a, or coefficient value a (Cva); model b, or coefficient value b (Cvb); and the novel model c or coef...
Chinese Journal of Practical Gynecology and Obstetrics, 2009
Journal of Integrative Agriculture, 2014
ABSTRACT To characterize the DNA rearrangement of both the T-DNA region and the genomic insertion... more ABSTRACT To characterize the DNA rearrangement of both the T-DNA region and the genomic insertion site during T-DNA insertion, the Genomewalker strategy was used to isolate the junctions between the inserted DNA and the plant genomic DNA in six rapeseed events as well as the genomic DNA at the sites before integration. During transformation in each of the six events, portions of both the right border (RB) and left border (LB) regions of the T-DNA were deleted, ranging from a 7 nucleotide deletion of the LB repeats in event RF1 to a 207 bp deletion of the LB region in event RF2. For the six events, T-DNA integration resulted in a deletion at the target site spanning less than 100 bp. Sequence analysis indicated that the T-DNA was integrated into the coding region of various native rapeseed genes in events RF1 and RF2. Duplications of the genomic DNA target site were observed in events RF2, RF3 and Topas 19/2. And multimerization of transgenes was found in event Topas 19/2, in which, the T-DNA was integrated as a head-to-head (RB-to-RB) concatemer into the recipient genome. In event MS1, chromosomal translocation or a large target-site deletion may have occurred during T-DNA integration, which was identified due to a failure to amplify the presumptive insertion site based on the flanking rapeseed DNA sequences. Our results provide comprehensive data concerning transgene organization and the genomic context of the T-DNA in six rapeseed events, which can aid in the developing of insert fingerprinting and the monitoring of long-term genetic stability and potential unintended effects of transgenic events.
Scientific reports, 2014
The Cauliflower mosaic virus (CaMV) 35S promoter (P35S) is a commonly used target for detection o... more The Cauliflower mosaic virus (CaMV) 35S promoter (P35S) is a commonly used target for detection of genetically modified organisms (GMOs). There are currently 24 reported detection methods, targeting different regions of the P35S promoter. Initial assessment revealed that due to the absence of primer binding sites in the P35S sequence, 19 of the 24 reported methods failed to detect P35S in MON88913 cotton, and the other two methods could only be applied to certain GMOs. The rest three reported methods were not suitable for measurement of P35S in some testing events, because SNPs in binding sites of the primer/probe would result in abnormal amplification plots and poor linear regression parameters. In this study, we discovered a conserved region in the P35S sequence through sequencing of P35S promoters from multiple transgenic events, and developed new qualitative and quantitative detection systems targeting this conserved region. The qualitative PCR could detect the P35S promoter in ...
Proceedings of the 2012 Third International Conference on Mechanic Automation and Control Engineering, Jul 27, 2012
Journal of Analytical Atomic Spectrometry, 1992
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, MARCH 1992, VOL. 7 ... Determination of Alkylselenides... more JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, MARCH 1992, VOL. 7 ... Determination of Alkylselenides by Gas Chromatography-Electrothermal Atomic Absorption Spectrometry* ... Jiang Gui-bin, Ni Zhe-ming,? Zhang Li, Li Ang, Han Heng-bin and Shan Xiao-quan ...
Annals of the Rheumatic Diseases, Mar 1, 2007
Journal of Agricultural and Food Chemistry, 2009
Zhonghua Yi Xue Za Zhi, Oct 1, 2003
To evaluate the inhibitory effect of human leukocyte antigen (HLA)-DRbeta1 specific collagen II (... more To evaluate the inhibitory effect of human leukocyte antigen (HLA)-DRbeta1 specific collagen II (CII) peptides with substitutions of TCR binding residues on T cell activation, and explore a new therapeutic strategy for T cell mediated autoimmune diseases by interfering with antigen recognition of T Cell receptor (TCR). Non-TCR binding peptides were designed by computer modeling based on interaction of HLA DR1. The modified CII263-272. Intracellular transfer of the modified CII peptide and its binding to HLA DR1 were studied using confocal microscopy and fluorescence-activated cell sorter (FACS). The effects of altered peptides on T cell activation were evaluated using an antigen presenting system consisting of HLA-DR1 transgenic APC and CII specific T cells. Computer modeling showed the side chains of 263 (F), 266 (E) fit in the peptide binding groove, and form hydrogen bond with alpha1, beta1 chain of HLA-DR1. The side chains of TCR specific 267 (Q) and 270 (K) protruded out of the groove, which might be TCR recognizing residues. The modified CII peptides with intact HLA-DR1 binding residues were bound to intracellular HLA-DR1 and expressed on cell surface. The modified peptides with single residue substitution of 267-270 and consecutive substitution of 268-270 showed a hyporesponsive T cell activation. Altered peptides 270A, sub268-270 could significantly suppress the T cell activation induced by CII263-272. The altered peptides with substitution of TCR binding residues are hyporesponsive in T cell activation, and may competitively inhibit the T cell activation in T cell mediated autoimmune diseases.
Journal of Agricultural and Food Chemistry, 2011
Transgenic rice TT51-1 (BT63) is an insect resistant strain that was granted for safety certifica... more Transgenic rice TT51-1 (BT63) is an insect resistant strain that was granted for safety certificate in China in 2009. This study characterizes the transgenic event TT51-1 using a GenomeWalker strategy. The organization of the transgenes indicated that the transgenes on two plasmids, pFHBT1 and pGL2RC7, had been integrated at the same locus. The sequence of the event TT51-1 spanned 8725 bp, including a truncated Cry1Ab/Ac cassette, an intact Cry1Ab/Ac cassette, two Amp gene segments, and an Hph gene segment. The 5' and 3' plant flanking sequences were isolated and used to locate the transgenes to chromosome 10 in TT51-1. The isolated TT51-1 fragment and a fragment of the rice PLD gene were integrated into a plasmid vector, to create plasmid pK-TT51 as a calibrator for detecting rice containing TT51-1. Analysis of unknown samples indicated that the reference plasmid was a reliable alternative to TT51-1 genomic DNA.
Theoretical and Applied Mechanics Letters, 2011
Advances in Intelligent and Soft Computing, 2011
Journal of Agricultural and Food Chemistry, 2010
JOURNAL OF INFRARED AND MILLIMETER WAVES
Continuous-wave terahertz imaging is demonstrated based on an optically-pumped far-infrared gas l... more Continuous-wave terahertz imaging is demonstrated based on an optically-pumped far-infrared gas laser and a Golay cell detector. The imaging system design and construction are discussed in the context of the choice and configuration of the involved terahertz optical components and devices. Performance characteristics of the system, including signal-to-noise ratio, spatial resolution, detector response and imaging speed are measured and analyzed in detail. With this system, terahertz transmission images of various samples are obtained, confirming the quality of our setup and supporting the feasibility of terahertz imaging technique for applications in security screening and quality control. A data processing method for automatic calibration of the image intensities in the background region is also proposed to remove the influence of laser power drift on the image quality.
Food Chemistry, 2010
The insect-resistant transgenic rice event TT51-1 (synonym BT63) has been found illicitly planted... more The insect-resistant transgenic rice event TT51-1 (synonym BT63) has been found illicitly planted and distributed for years although it has never been approved for commercial cultivation in any country up to now. The purpose of this study was to establish a detection method that is specific for this transformation event. The event-specific PCR method produces an amplicon of 120 basepairs (bp) based on the revealed 3 0 junction sequence with a limit of detection (LOD) and a limit of quantification (LOQ) being approximately 5 and 10 initial template copies, respectively. Two mixed rice samples with known TT51-1 contents were used to verify the developed real-time PCR system, from which the expected results were observed.
Journal of Wuhan University of Technology-Mater. Sci. Ed., 2010
Advances in Mechanical Engineering, Aug 21, 2013
Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji, 2015
The development of the skeleton is regulated by numerous signaling molecules expressed in epiphys... more The development of the skeleton is regulated by numerous signaling molecules expressed in epiphyseal cartilage controlling both chondrogenesis and osteogenesis such as fibroblast growth factor receptors (FGFRs). In order to explore the important effect of fibroblast growth factor receptor 2 (FGFR2) in the process of mandibular condylar growth, we introduced gain-of-function Fgfr2(+/S252W) mice, and investigated mandibular condylar morphology by means of safranin-o/fast green staining at the stage of 1 week, 3 weeks and 6 weeks. The mutant mice displayed narrower width of the mandibular condylar growth plate, stronger stainings of trabecular bone at the stage of 1 week, 3 weeks and 6 weeks and faster degradation of the calcified cartilage cell layer at the stage of 6 weeks. We also assessed the expression of type X collagen (Col X) in mandibular condyle at the stage of 3 weeks by immunohistochemical staining and real-time PCR. The results showed that Col X was increased in the mutant...
Analytical and bioanalytical chemistry, 2014
Plasmid calibrators are increasingly applied for polymerase chain reaction (PCR) analysis of gene... more Plasmid calibrators are increasingly applied for polymerase chain reaction (PCR) analysis of genetically modified organisms (GMOs). To evaluate the commutability between plasmid DNA (pDNA) and genomic DNA (gDNA) as calibrators, a plasmid molecule, pBSTopas, was constructed, harboring a Topas 19/2 event-specific sequence and a partial sequence of the rapeseed reference gene CruA. Assays of the pDNA showed similar limits of detection (five copies for Topas 19/2 and CruA) and quantification (40 copies for Topas 19/2 and 20 for CruA) as those for the gDNA. Comparisons of plasmid and genomic standard curves indicated that the slopes, intercepts, and PCR efficiency for pBSTopas were significantly different from CRM Topas 19/2 gDNA for quantitative analysis of GMOs. Three correction methods were used to calibrate the quantitative analysis of control samples using pDNA as calibrators: model a, or coefficient value a (Cva); model b, or coefficient value b (Cvb); and the novel model c or coef...
Chinese Journal of Practical Gynecology and Obstetrics, 2009
Journal of Integrative Agriculture, 2014
ABSTRACT To characterize the DNA rearrangement of both the T-DNA region and the genomic insertion... more ABSTRACT To characterize the DNA rearrangement of both the T-DNA region and the genomic insertion site during T-DNA insertion, the Genomewalker strategy was used to isolate the junctions between the inserted DNA and the plant genomic DNA in six rapeseed events as well as the genomic DNA at the sites before integration. During transformation in each of the six events, portions of both the right border (RB) and left border (LB) regions of the T-DNA were deleted, ranging from a 7 nucleotide deletion of the LB repeats in event RF1 to a 207 bp deletion of the LB region in event RF2. For the six events, T-DNA integration resulted in a deletion at the target site spanning less than 100 bp. Sequence analysis indicated that the T-DNA was integrated into the coding region of various native rapeseed genes in events RF1 and RF2. Duplications of the genomic DNA target site were observed in events RF2, RF3 and Topas 19/2. And multimerization of transgenes was found in event Topas 19/2, in which, the T-DNA was integrated as a head-to-head (RB-to-RB) concatemer into the recipient genome. In event MS1, chromosomal translocation or a large target-site deletion may have occurred during T-DNA integration, which was identified due to a failure to amplify the presumptive insertion site based on the flanking rapeseed DNA sequences. Our results provide comprehensive data concerning transgene organization and the genomic context of the T-DNA in six rapeseed events, which can aid in the developing of insert fingerprinting and the monitoring of long-term genetic stability and potential unintended effects of transgenic events.
Scientific reports, 2014
The Cauliflower mosaic virus (CaMV) 35S promoter (P35S) is a commonly used target for detection o... more The Cauliflower mosaic virus (CaMV) 35S promoter (P35S) is a commonly used target for detection of genetically modified organisms (GMOs). There are currently 24 reported detection methods, targeting different regions of the P35S promoter. Initial assessment revealed that due to the absence of primer binding sites in the P35S sequence, 19 of the 24 reported methods failed to detect P35S in MON88913 cotton, and the other two methods could only be applied to certain GMOs. The rest three reported methods were not suitable for measurement of P35S in some testing events, because SNPs in binding sites of the primer/probe would result in abnormal amplification plots and poor linear regression parameters. In this study, we discovered a conserved region in the P35S sequence through sequencing of P35S promoters from multiple transgenic events, and developed new qualitative and quantitative detection systems targeting this conserved region. The qualitative PCR could detect the P35S promoter in ...
Proceedings of the 2012 Third International Conference on Mechanic Automation and Control Engineering, Jul 27, 2012
Journal of Analytical Atomic Spectrometry, 1992
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, MARCH 1992, VOL. 7 ... Determination of Alkylselenides... more JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, MARCH 1992, VOL. 7 ... Determination of Alkylselenides by Gas Chromatography-Electrothermal Atomic Absorption Spectrometry* ... Jiang Gui-bin, Ni Zhe-ming,? Zhang Li, Li Ang, Han Heng-bin and Shan Xiao-quan ...
Annals of the Rheumatic Diseases, Mar 1, 2007
Journal of Agricultural and Food Chemistry, 2009
Zhonghua Yi Xue Za Zhi, Oct 1, 2003
To evaluate the inhibitory effect of human leukocyte antigen (HLA)-DRbeta1 specific collagen II (... more To evaluate the inhibitory effect of human leukocyte antigen (HLA)-DRbeta1 specific collagen II (CII) peptides with substitutions of TCR binding residues on T cell activation, and explore a new therapeutic strategy for T cell mediated autoimmune diseases by interfering with antigen recognition of T Cell receptor (TCR). Non-TCR binding peptides were designed by computer modeling based on interaction of HLA DR1. The modified CII263-272. Intracellular transfer of the modified CII peptide and its binding to HLA DR1 were studied using confocal microscopy and fluorescence-activated cell sorter (FACS). The effects of altered peptides on T cell activation were evaluated using an antigen presenting system consisting of HLA-DR1 transgenic APC and CII specific T cells. Computer modeling showed the side chains of 263 (F), 266 (E) fit in the peptide binding groove, and form hydrogen bond with alpha1, beta1 chain of HLA-DR1. The side chains of TCR specific 267 (Q) and 270 (K) protruded out of the groove, which might be TCR recognizing residues. The modified CII peptides with intact HLA-DR1 binding residues were bound to intracellular HLA-DR1 and expressed on cell surface. The modified peptides with single residue substitution of 267-270 and consecutive substitution of 268-270 showed a hyporesponsive T cell activation. Altered peptides 270A, sub268-270 could significantly suppress the T cell activation induced by CII263-272. The altered peptides with substitution of TCR binding residues are hyporesponsive in T cell activation, and may competitively inhibit the T cell activation in T cell mediated autoimmune diseases.
Journal of Agricultural and Food Chemistry, 2011
Transgenic rice TT51-1 (BT63) is an insect resistant strain that was granted for safety certifica... more Transgenic rice TT51-1 (BT63) is an insect resistant strain that was granted for safety certificate in China in 2009. This study characterizes the transgenic event TT51-1 using a GenomeWalker strategy. The organization of the transgenes indicated that the transgenes on two plasmids, pFHBT1 and pGL2RC7, had been integrated at the same locus. The sequence of the event TT51-1 spanned 8725 bp, including a truncated Cry1Ab/Ac cassette, an intact Cry1Ab/Ac cassette, two Amp gene segments, and an Hph gene segment. The 5' and 3' plant flanking sequences were isolated and used to locate the transgenes to chromosome 10 in TT51-1. The isolated TT51-1 fragment and a fragment of the rice PLD gene were integrated into a plasmid vector, to create plasmid pK-TT51 as a calibrator for detecting rice containing TT51-1. Analysis of unknown samples indicated that the reference plasmid was a reliable alternative to TT51-1 genomic DNA.
Theoretical and Applied Mechanics Letters, 2011
Advances in Intelligent and Soft Computing, 2011
Journal of Agricultural and Food Chemistry, 2010
JOURNAL OF INFRARED AND MILLIMETER WAVES
Continuous-wave terahertz imaging is demonstrated based on an optically-pumped far-infrared gas l... more Continuous-wave terahertz imaging is demonstrated based on an optically-pumped far-infrared gas laser and a Golay cell detector. The imaging system design and construction are discussed in the context of the choice and configuration of the involved terahertz optical components and devices. Performance characteristics of the system, including signal-to-noise ratio, spatial resolution, detector response and imaging speed are measured and analyzed in detail. With this system, terahertz transmission images of various samples are obtained, confirming the quality of our setup and supporting the feasibility of terahertz imaging technique for applications in security screening and quality control. A data processing method for automatic calibration of the image intensities in the background region is also proposed to remove the influence of laser power drift on the image quality.
Food Chemistry, 2010
The insect-resistant transgenic rice event TT51-1 (synonym BT63) has been found illicitly planted... more The insect-resistant transgenic rice event TT51-1 (synonym BT63) has been found illicitly planted and distributed for years although it has never been approved for commercial cultivation in any country up to now. The purpose of this study was to establish a detection method that is specific for this transformation event. The event-specific PCR method produces an amplicon of 120 basepairs (bp) based on the revealed 3 0 junction sequence with a limit of detection (LOD) and a limit of quantification (LOQ) being approximately 5 and 10 initial template copies, respectively. Two mixed rice samples with known TT51-1 contents were used to verify the developed real-time PCR system, from which the expected results were observed.
Journal of Wuhan University of Technology-Mater. Sci. Ed., 2010
Advances in Mechanical Engineering, Aug 21, 2013
Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji, 2015
The development of the skeleton is regulated by numerous signaling molecules expressed in epiphys... more The development of the skeleton is regulated by numerous signaling molecules expressed in epiphyseal cartilage controlling both chondrogenesis and osteogenesis such as fibroblast growth factor receptors (FGFRs). In order to explore the important effect of fibroblast growth factor receptor 2 (FGFR2) in the process of mandibular condylar growth, we introduced gain-of-function Fgfr2(+/S252W) mice, and investigated mandibular condylar morphology by means of safranin-o/fast green staining at the stage of 1 week, 3 weeks and 6 weeks. The mutant mice displayed narrower width of the mandibular condylar growth plate, stronger stainings of trabecular bone at the stage of 1 week, 3 weeks and 6 weeks and faster degradation of the calcified cartilage cell layer at the stage of 6 weeks. We also assessed the expression of type X collagen (Col X) in mandibular condyle at the stage of 3 weeks by immunohistochemical staining and real-time PCR. The results showed that Col X was increased in the mutant...