Thirumalai Ellayavilli | Annamalai university, chidambaram (original) (raw)
Papers by Thirumalai Ellayavilli
ADVANCES IN BIORESEARCH, Society of Education, India, 2024
Inflammation is one of the most important immune reactions that may lead to arthritis, allergies,... more Inflammation is one of the most important immune reactions that may lead to arthritis, allergies, respiratory diseases,
cancer, etc. Medicinal plants are recognized as one of the promising sources of novel anti-inflammatory drugs that can
overcome the adverse side effects associated with conventional anti-inflammatory drugs. Solanum xanthocarpum, Sida
cardifolia, and Psidium guajava are indigenous edible herbs that were extensively used to treat various diseases. The
leaves of S. cardifolia and P.guajava with S.xanthocarpum fruits were mixed in 1:1:1 & 2:1:1 ratios respectively. The
herbal mixture was subjected to the preparation of extract through a cold maceration technique using ethanol (Eth) and
water (Aq) as solvents. The polyherbal formulations (PHF) were named Aq1:1:1, Aq2:1:1, Eth1:1:1, and Eth2:1:1. The
total phenolic (TPC) and flavonoid content (TFC) were estimated using a biochemical method, and the antiinflammatory activity was analyzed by inhibition of protein denaturation and SRBC membrane stabilization assays. The
data were statistically analyzed by ANOVA and post hoc test (Bonferroni test). The results revealed that the Eth2:1:1 of
PHF had the highest TPC (89.34 mg GAE/100g of Dry ext) & TFC (59.58 mg QE/100g of Dry ext). Similarly, the antiinflammatory assays also showed that Eth2:1:1 had the highest percentage in SRBC membrane stabilization (66.7±1.2)
and Protein denaturation inhibitory (60.9±1.7) assays than other PHF forms. Hence the current study exhibits the better
anti-inflammatory effect of novel PHF with the 2:1:1 ratio of ethanolic extract. This finding may help to develop a new
drug to treat various life-threatening inflammatory conditions
IJPSR, 2021
Cadaba indica Lam (Indian Cadaba), an Indian traditional medicinal plant has been used for variou... more Cadaba indica Lam (Indian Cadaba), an Indian traditional medicinal plant has been used for various diseases. The cold macerated methanolic leaf extract of Cadaba indica was assessed using Agilent GC 7890A gas chromatography connected with an MS-5975C mass spectrometer and the mass spectra were matched with NIST 14.0data library. Several chemical constituents were identified within 28 min of the entire GC-MS analysis. The GC chromatogram shows that presence of most abundant linolenic acids and its esters such as Hexa-decanoic acid, methyl ester, n-hexadecanoic acid,
Two different types of methanolic leaf extracts of Cadaba indica Lam were studied to prove their ... more Two different types of methanolic leaf extracts of Cadaba indica Lam were studied to prove their anti-arthritic activity. Cadaba indica is a traditional medicinal plant used in folklore for analgesic, anti-inflammatory and antimicrobial actions. In this study, Cadaba indica leaves extract was obtained by hot percolation and cold maceration methods. The Invitro anti-arthritic activity of hot percolation methanolic leaf extract (CIHME) and cold maceration methanolic extract (CICME) were studied by 'anti proteinase' and 'protein denaturation inhibition' assays. Both extracts were then compared with the standard drug dexamethasone. Different concentrations (100, 200, 400, 800 and 1000µg/ml) of the extract and that of the standard drug were prepared and were analyzed. The results showed that both CICME and CIHME extract effectively inhibited protein denaturation and proteinase enzyme activity in a dose dependent manner. Actions of both extracts were statistically significant when compared with the standard drug dexamethasone, although CICME resulted in a greater 'percentage inhibition' than CIHME. Dunnet's't' test was used for data analysis. Our study showed that CICME was more effective and more potent than CIHME. This may be due to a high concentration of bioactive compounds like quercetin, gallic acid and rutin in CICME.
Cadaba indica Lam. is one of the medicinal plants used in Indian traditional systems of medicine ... more Cadaba indica Lam. is one of the medicinal plants used in Indian traditional systems of medicine for the treatment of various diseases of mankind. The present study mainly aimed to estimate the content of major constituent flavonoid present in various extracts of leaves of Cadaba indica collected from Tamilnadu. HPTLC method was adopted for determining the content of the active constituent present in the various extracts using marker compound Quercetin, rutin and gallic acid. The HPTLC method was performed using HPTLC aluminium sheets precoated with silica gel 60 GF 254 as stationary phase and mobile phase as toluene: ethyl acetate: formic acid: methanol (3:6:1.6:0.4). The developed chromatogram was scanned at 254 nm using Camag scanner III. The chromatogram obtained and the peak profile of the components collected by scanning could made up the fingerprint of the various extracts of Cadaba indica leaves compared with antioxidants markers of flavonoids and phenolic acids. HPTLC finger print analysis showed that the presence of rutin, quercetin and gallic acid which may responsible for the anti-oxidant activity mentioned. This method is appropriate to determine the percentage of flavonoids and phenolic acids in various extracts of leaves of Cadaba indica.
ADVANCES IN BIORESEARCH, Society of Education, India, 2024
Inflammation is one of the most important immune reactions that may lead to arthritis, allergies,... more Inflammation is one of the most important immune reactions that may lead to arthritis, allergies, respiratory diseases,
cancer, etc. Medicinal plants are recognized as one of the promising sources of novel anti-inflammatory drugs that can
overcome the adverse side effects associated with conventional anti-inflammatory drugs. Solanum xanthocarpum, Sida
cardifolia, and Psidium guajava are indigenous edible herbs that were extensively used to treat various diseases. The
leaves of S. cardifolia and P.guajava with S.xanthocarpum fruits were mixed in 1:1:1 & 2:1:1 ratios respectively. The
herbal mixture was subjected to the preparation of extract through a cold maceration technique using ethanol (Eth) and
water (Aq) as solvents. The polyherbal formulations (PHF) were named Aq1:1:1, Aq2:1:1, Eth1:1:1, and Eth2:1:1. The
total phenolic (TPC) and flavonoid content (TFC) were estimated using a biochemical method, and the antiinflammatory activity was analyzed by inhibition of protein denaturation and SRBC membrane stabilization assays. The
data were statistically analyzed by ANOVA and post hoc test (Bonferroni test). The results revealed that the Eth2:1:1 of
PHF had the highest TPC (89.34 mg GAE/100g of Dry ext) & TFC (59.58 mg QE/100g of Dry ext). Similarly, the antiinflammatory assays also showed that Eth2:1:1 had the highest percentage in SRBC membrane stabilization (66.7±1.2)
and Protein denaturation inhibitory (60.9±1.7) assays than other PHF forms. Hence the current study exhibits the better
anti-inflammatory effect of novel PHF with the 2:1:1 ratio of ethanolic extract. This finding may help to develop a new
drug to treat various life-threatening inflammatory conditions
IJPSR, 2021
Cadaba indica Lam (Indian Cadaba), an Indian traditional medicinal plant has been used for variou... more Cadaba indica Lam (Indian Cadaba), an Indian traditional medicinal plant has been used for various diseases. The cold macerated methanolic leaf extract of Cadaba indica was assessed using Agilent GC 7890A gas chromatography connected with an MS-5975C mass spectrometer and the mass spectra were matched with NIST 14.0data library. Several chemical constituents were identified within 28 min of the entire GC-MS analysis. The GC chromatogram shows that presence of most abundant linolenic acids and its esters such as Hexa-decanoic acid, methyl ester, n-hexadecanoic acid,
Two different types of methanolic leaf extracts of Cadaba indica Lam were studied to prove their ... more Two different types of methanolic leaf extracts of Cadaba indica Lam were studied to prove their anti-arthritic activity. Cadaba indica is a traditional medicinal plant used in folklore for analgesic, anti-inflammatory and antimicrobial actions. In this study, Cadaba indica leaves extract was obtained by hot percolation and cold maceration methods. The Invitro anti-arthritic activity of hot percolation methanolic leaf extract (CIHME) and cold maceration methanolic extract (CICME) were studied by 'anti proteinase' and 'protein denaturation inhibition' assays. Both extracts were then compared with the standard drug dexamethasone. Different concentrations (100, 200, 400, 800 and 1000µg/ml) of the extract and that of the standard drug were prepared and were analyzed. The results showed that both CICME and CIHME extract effectively inhibited protein denaturation and proteinase enzyme activity in a dose dependent manner. Actions of both extracts were statistically significant when compared with the standard drug dexamethasone, although CICME resulted in a greater 'percentage inhibition' than CIHME. Dunnet's't' test was used for data analysis. Our study showed that CICME was more effective and more potent than CIHME. This may be due to a high concentration of bioactive compounds like quercetin, gallic acid and rutin in CICME.
Cadaba indica Lam. is one of the medicinal plants used in Indian traditional systems of medicine ... more Cadaba indica Lam. is one of the medicinal plants used in Indian traditional systems of medicine for the treatment of various diseases of mankind. The present study mainly aimed to estimate the content of major constituent flavonoid present in various extracts of leaves of Cadaba indica collected from Tamilnadu. HPTLC method was adopted for determining the content of the active constituent present in the various extracts using marker compound Quercetin, rutin and gallic acid. The HPTLC method was performed using HPTLC aluminium sheets precoated with silica gel 60 GF 254 as stationary phase and mobile phase as toluene: ethyl acetate: formic acid: methanol (3:6:1.6:0.4). The developed chromatogram was scanned at 254 nm using Camag scanner III. The chromatogram obtained and the peak profile of the components collected by scanning could made up the fingerprint of the various extracts of Cadaba indica leaves compared with antioxidants markers of flavonoids and phenolic acids. HPTLC finger print analysis showed that the presence of rutin, quercetin and gallic acid which may responsible for the anti-oxidant activity mentioned. This method is appropriate to determine the percentage of flavonoids and phenolic acids in various extracts of leaves of Cadaba indica.