laila refahy | Theodor Bilharze Research Institute (original) (raw)

Papers by laila refahy

Planta Medica, Oct 30, 2014

Egyptian Journal of Chemistry, Jul 8, 2021

The current study aims to evaluate the antioxidant, anticancer and antiviral activities of Phylla... more The current study aims to evaluate the antioxidant, anticancer and antiviral activities of Phyllanthus emblica leaves, as well as characterization of bioactive chemical constituents. The total phenolic and flavonoid contents of plant extracts were determined using Folin-Ciocalteu's and aluminium trichloride assays, respectively. The antioxidant activities were evaluated by different methods, the anticancer activity was evaluated via MTT assay, while the antiviral activity was evaluated using cytopathic effect (CPE) inhibition and anti-HCV assays. Moreover, the chemical profiling of extracts was performed using reversed phase high-performance liquid-chromatography method. Our finding revealed that P. emblica is rich in glycosides, flavonoids, phenolics, terpenoids and tannins. The n-butanol fraction exhibited the highest total phenolic and flavonoid contents with values 654.78 mg GAE /g ext and 110.14 mg RE / g ext, respectively. Also, it possessed the most effective antioxidant activities (DPPH; IC50=19.59 µg/mL, RPAA; 0.948 mg AAE /g ext., and TAC; 543.62mg AAE /g ext.) and anticancer activity against HepG-2 and MCF-7 cell lines with an IC50 of 25.63 and 22.80μg/mL, respectively. Moreover, P. emblica extracts exhibited anti-HCV activity. The HPLC finger print results of P. emblica extracts showed 14 peaks superimposed to the standards. Structure elucidation of pure isolates was achieved via IR, UV, 1 H & 13 C-NMR

Journal of Pharmacy and Pharmacology, Nov 10, 2017

Objectives The secondary metabolites of the fruits of Firmiana simplex (L.) were analysed by LC-D... more Objectives The secondary metabolites of the fruits of Firmiana simplex (L.) were analysed by LC-DAD-ESI-MS/MS; furthermore, we evaluated their antioxidant and antigenotoxic properties. Methods The antioxidant activity was investigated using the 2,2 0-diphenyl-1picrylhydrazyl radical (DPPH), the 2,2 0-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) and the ferric reducing antioxidant power (FRAP) assays. The antigenotoxic potential was determined via the comet assay. Key findings The ethyl acetate fraction (EtOAc) was analysed by LC-DAD-ESI-MS/MS: phenolic acids and flavonoids were the main polyphenols of the fruits. The EtOAc fraction yielded the highest content of polyphenols with 314.61 mg GAE/g extract, followed by 297.51, 153.75, 101.47, 97.19 for dichloromethane, butanol, methanol and water extracts, respectively. As expected, a strong correlation exists between the antioxidant activity of the investigated extracts and their total phenolic content. In the DPPH assay, the IC 50 value of the most active EtOAc fraction was 6.79 lg/ml, relative to 2.92 lg/ml of the standard ascorbic acid. ABTS and FRAP assays supported the results of DPPH assay. Moreover, using the comet assay, we could show that the phenol-rich EtOAc extract exhibits an antigenotoxic potential in human liver cancer cells (Hep-G2) treated with hydrogen peroxide (H 2 O 2) as a genotoxic agent. Conclusions The fruits of Firmiana simplex may be a good natural source of antioxidant and antigenotoxic agents.

Egyptian Journal of Chemistry

Egyptian journal of bilharziasis, 1982

L'etude comparative de la sensibilite de Biomphalaria alexandrina et Bulinus truncatus a l&#3... more L'etude comparative de la sensibilite de Biomphalaria alexandrina et Bulinus truncatus a l'action de saponines dans des extraits aqueux de Agave angustifolia, A. celsii et Yucca filamentosa est determinee. L'action de quelques facteurs de l'environnement sur l'activite molluscicide de ces saponines est recherchee

Egyptian journal of bilharziasis, 1994

Journal of the Egyptian …, 1984

1. J Egypt Soc Parasitol. 1984 Jun;14(1):265-73. The molluscicidal properties of Agave decepiens ... more 1. J Egypt Soc Parasitol. 1984 Jun;14(1):265-73. The molluscicidal properties of Agave decepiens and Agave americana (var. marginata). Shoeb HA, Hassan AA, el-Sayed MM, Refahy L. PMID: 6736709 [PubMed - indexed for MEDLINE] MeSH Terms: ...

4 Abstract: The present study investigated the potential antischistosomal activity of some Egypti... more 4 Abstract: The present study investigated the potential antischistosomal activity of some Egyptian native plants using the in vitro Schistosoma mansoni worm killing assay. Praziquantel (PZQ) treated worms were used as positive control. Key findings: The bioscreening results revealed 27 extracts out of 90 different extracts from the 65 examined plant species possess reproducible in vitro antischistosomal activity. The LC of latex 50 methanol extract of Calotropis procera was 0.30 µg/ml, while the LC of the latex water extract was 0.59 µg/ml 50 compared to 0.21 µg/ml for PZQ. LC of latex water extract of Calotropis procera increased to 3.22 µg/ml. 50 Phytochemical screening of different extracts was carried out to detect extracts major chemical constituents responsible for activity. Other species from families "Fabeace, Asparagaceae, Pittosporaceae, Balanitaceae, Zingiberaceae and Lauraceae" showed variable percentages of worm killing activities (10%-100%). Conclusion: The ...

Current Bioactive Compounds, 2020

Background: Desert truffles (Terfezia species) are known by their vital nutritional benefits as t... more Background: Desert truffles (Terfezia species) are known by their vital nutritional benefits as they are considered as rich sources of vitamins, fatty acid, minerals and proteins. Methods: The chemical constituents of the different solvent extracts of Terfezia species were isolated and identified by column chromatography, spectroscopic and GC-MS analyses. Also, the ethyl acetate and acetone extracts of different fungal isolates, associated Terfezia, after grown on rice medium were screened for their antimicrobial, anticancer and antioxidant activities via disc agar plate, micro culture tetrazolium (MTT) and 2,2-azino-di-[3-ethylbenzo-thiazolin-sulphonate] (ABTS) assays, respectively. The promising fugal strains were molecularly identified by 18SrRNA tool. Results: Bio-guided separation of methylene chloride, ethyl acetate and n-butanol fractions of Terfezia species led to identification of nine compounds namely; (R)-4,8-dihydroxy-7-hydroxymethyl-6- methoxy isochroman-1-one (1), 4-de...

Asian Journal of Pharmaceutical and Clinical Research, 2016

Objective: To evaluate the antioxidant activity and chemical composition of Punica granatum L. an... more Objective: To evaluate the antioxidant activity and chemical composition of Punica granatum L. and test it as immunostimulants against Schistosoma mansoni infection to Biomphalaria alexandrina snails. Methods: Antioxidant activity was evaluated by measuring the free radical scavenging activity of the 90% defatted methanol extract (90% DM) of P. granatum peels and its sub-derived fractions was evaluated via 2,2’-diphenyl-1-picrylhydrazyl and its chemical constituents were identified via gas chromatography-mass spectrometry (GC-MS) analysis. B. alexandrina snails were exposed to pomegranate extracts (PEs) for 1 month before their challenging with S. mansoni miracidia. Infection rates, immunological and histological parameters were, then, evaluated in PE-exposed snails and compared to controls. R esults: The antioxidants activities of PE, expressed as scavenging concentration at 50%, were in the following order; 90% DM (12.45) ˃ n -butanol(15.59) ˃ethyl acetate (21.36) ˃water (49.16) µ...

Oriental Journal of Chemistry, 2015

Cancer is the abnormal growth of cells that can lead to death. According to the American Cancer S... more Cancer is the abnormal growth of cells that can lead to death. According to the American Cancer Society, deaths arising from cancer constitute 2-3% of the annual deaths recorded worldwide. Although there are many kinds of cancer, they all have in common this out-of-control growth of cells 1. Hepatocellular carcinoma (HCC) is a

2 Abstract: A bioassay-guided fractionation and chemical investigation of the Gmelina arborea lea... more 2 Abstract: A bioassay-guided fractionation and chemical investigation of the Gmelina arborea leaves (Verbenaceae) resulted in the isolation and characterization of six known flavonoids (1, 3-7) along with new flavone glycoside (2), identified as; luteolin (1), luteolin-4'-O- -D- C -galactoside (2), kaempferol (3), quercetin - 4 1

The essential oil from the fresh leaves of Eucalyptus citriodora Hook. (Myrtaceae) was isolated v... more The essential oil from the fresh leaves of Eucalyptus citriodora Hook. (Myrtaceae) was isolated via hydrodistillation technique and was analyzed by gas chromatography-mass spectrometry (GC-MS), yield (1.0% v/w). Thirteen compounds were identified in the essential oil representing 88.76% of the total oil composition. Citronellol (33.52%), pulegol (25.20%) and citronellyl acetate (14.70%) were the major components. The antioxidant activity was carried out quantitatively via 1,1'-diphenyl-2-picraylhydrazyl free radical and qualitatively via dot-blot and DPPH staining methods. The anticancer activity was carried out via MTT assay using three human tumor cell lines namely; hepatocellular carcinoma (liver) HePG-2, mammary gland (breast) MCF-7 and colorectal carcinoma (colon) HCT-116. The antioxidant results revealed that Eucalyptus citriodora oil showed antioxidant activity against DPPH radical (SC50= 32.58 μg/ml) in comparison with ascorbic acid as standard (SC50= 7.0 μg/ml), also qu...

2 Abstract: Terminalia arjuna, a well-known medicinal plant has extensively been used in Ayurvedi... more 2 Abstract: Terminalia arjuna, a well-known medicinal plant has extensively been used in Ayurvedic medicines to treat various diseases. The present study was investigated the phenolic content and antioxidant activity of 70% acetone extract of Terminalia arjuna and to isolate and identify its certain phenolic constituents as well as assessment the antioxidant potential of purified compounds via two qualitative in vitro antioxidant assays (1,1'-diphenyl-2-picrylhydrazyl) free radical scavenging (DPPH) and phosphomolybdenum as wall as total phenolic content was determined via Folin-Ciocalteu assay. Owing to the high antioxidant of the 70% acetone extract it undergoes successive fractionation via; diethyl ether, petroleum ether, CH Cl , EtOAc and n-BuOH. 22 n-BuOH sub-fraction undergoes chromatographic separation upon polyamide CC. to afforded three compounds (1-3), their structures were established via UV, IR, H and C-NMR analyses and identified as; gallic acid (1), 1 13 methyl gal...

Pharmacognosy Research

Background: Strelitzia nicolai Regel and Körn (Strelitziaceae) is native to Southern Africa whose... more Background: Strelitzia nicolai Regel and Körn (Strelitziaceae) is native to Southern Africa whose phytochemistry and pharmacology were slightly investigated. Materials and Methods: In the current work, different solvent extracts of S. nicolai were screened for their chemical profiles through high-performance liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS/MS) analyses. Furthermore, their in vitro antioxidant, cytotoxic, and anticancer activities were evaluated using 2,2'-diphenyl-1-picrylhydrazyl radical (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) & ferric reducing antioxidant power (FRAP) and crystal violet staining (CVS) colorimetric assays, respectively. Results: HPLC-DAD-ESI-MS/MS analyses led to the identification of nineteen and eleven phenolic compounds from the ethyl acetate and n-butanol extracts, respectively including flavonoids (e.g., quercetin 3-(2 G-rhamnosylrutinoside, quercetin, quercetin-3-O-glucoside, kaempferol-3,7-O-dirhamnoside, isorhamnetin-3-O-rutinoside and kaempferol-3-O-glucoside), phenolic acids derivatives (e.g., chlorogenic acid glycoside, protocatechuic acid-O-glucoside and caftaric acid), chalcones (e.g., xanthoangelol), and phenylethanoids (e.g., ligstroside glucoside). Moreover, in the DPPH assay the IC50value of the most active ethyl acetate extract was 20.49 μg/mL, relative to 2.92 μg/mL of ascorbic acid. ABTS and FRAP results reinforced the results of DPPH assay. According to the National Cancer Institute criteria, the tested extracts showed weak to moderate cytotoxic activities with IC50values ranged from 65.23 to 451.29 μg/mL. Furthermore, the EtOAc and n-BuOH extracts showed a noticeable anticancer activity with CVS spectroscopic readings for liver hepatocellular carcinoma growth 0.806 and 0.684 at a concentration (125 μg/mL), as well as 0.730 and 0.618 at concentration (500 μg/mL), respectively against control at 1.022. Conclusion: The obtained results reveal the high efficacy of the phenolic-rich extracts from S. nicolai as naturally occurring antioxidant and anti-tumor agents. Abbreviations Used: HPLC-DAD-ESI-MS/MS: High-performance liquid chromatography-diode array detection-electrospray ionization-mass/mass; DPPH: 2,2'-Diphenyl-1-picrylhydrazyl radical; ABTS: 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulphonic acid); FRAP: Ferric reducing antioxidant power; TPTZ: Tripyridyl-s-triazine; FE: Ferrous equivalents; DMEM: Dulbecco's modified eagle's medium; DMSO: Dimethyl sulfoxide; EDTA: Ethylenediaminetetraacetic acid; PBS: Phosphate buffered saline; HepG-2: Liver hepatocellular carcinoma; CVS: Crystal violet stain; NCI: National cancer institute; Glu: Glucose; Rha: Rhamnose.

Acta Chromatographica

Eight compounds were isolated and identified from the soil-inhabiting fungus Aspergillus fumigatu... more Eight compounds were isolated and identified from the soil-inhabiting fungus Aspergillus fumigatus 3T-EGY, namely, stearic acid (1), α-linolenic acid (2), physcion (3), di-(2-ethylhexyl) phthalate (4), 2,4,5,17-tetramethoxy pradimicin lactone (5), 3,5-dihydroxy-7-O-α-rhamnopyranoyl-2H-chromen-2-one (6), juglanthraquinone A-5-O-d-rhodosamine-(4′→1″)-2-deoxy-d-glucose (4″→1″′)-cinerulose B (7), and micropeptin (8). Their structures were determined on the basis of one-dimensional (1D-) and two-dimensional nuclear magnetic resonance (2D-NMR) [1H-, 13C-NMR, 1H-1H COSY (COrrelated SpectroscopY), and 1H-13C HMBC (Heteronuclear Multiple Bond Correlation) spectroscopy]. Compound 7 showed moderate in vitro antimicrobial activity against three pathogenic strains with inhibition zones values were ranged from 9.0 to 10.66 mm compared to neomycin as a positive control with inhibition zones values were ranged from 14.0 to 19.0 mm.

The Journal of pharmacy and pharmacology, Jan 10, 2017

The secondary metabolites of the fruits of Firmiana simplex (L.) were analysed by LC-DAD-ESI-MS/M... more The secondary metabolites of the fruits of Firmiana simplex (L.) were analysed by LC-DAD-ESI-MS/MS; furthermore, we evaluated their antioxidant and antigenotoxic properties. The antioxidant activity was investigated using the 2,2'-diphenyl-1-picrylhydrazyl radical (DPPH), the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) and the ferric reducing antioxidant power (FRAP) assays. The antigenotoxic potential was determined via the comet assay. The ethyl acetate fraction (EtOAc) was analysed by LC-DAD-ESI-MS/MS: phenolic acids and flavonoids were the main polyphenols of the fruits. The EtOAc fraction yielded the highest content of polyphenols with 314.61 mg GAE/g extract, followed by 297.51, 153.75, 101.47, 97.19 for dichloromethane, butanol, methanol and water extracts, respectively. As expected, a strong correlation exists between the antioxidant activity of the investigated extracts and their total phenolic content. In the DPPH assay, the IC50 ...