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Research paper thumbnail of The impact of BCL-2 overexpression on product mab glycosylation hybridoma cultures

Research paper thumbnail of The physiology of the Skin

Page 1. The physiology of the Skin By Dr Fadzilah Adibah Abdul Majid A Three Day Hands-On Course ... more Page 1. The physiology of the Skin By Dr Fadzilah Adibah Abdul Majid A Three Day Hands-On Course on Household and Personal Care Product Formulations 17-19 May 2004 CEPP, UTM Page 2. The skin is a window through which the physician can "see" the entire body ...

Research paper thumbnail of Educating Engineers: Contribution Of Muslim Women EducatorsIn Universiti Teknologi Malaysia

Research paper thumbnail of Anti-Inflammatory Activity of Selected Edible Herbs and Spices on Cultured Human Gingival Fibroblasts

The Open Conference Proceedings Journal, 2013

Research paper thumbnail of Degradation and in vitro cell–material interaction studies on hydroxyapatite-coated biodegradable porous iron for hard tissue scaffolds

Journal of Orthopaedic Translation, 2014

Research paper thumbnail of Anti-Bacterial Activity of Cinnamon Oil on Oral Pathogens

The Open Conference Proceedings Journal, 2013

Research paper thumbnail of Pineapple Branding and Biorefinery Program(PBBP)

Page 1. Pineapple Branding and Biorefinery Program (PBBP) Fadzilah Adibah bt Hj. Abdul Majid, PhD... more Page 1. Pineapple Branding and Biorefinery Program (PBBP) Fadzilah Adibah bt Hj. Abdul Majid, PhD Bioprocess Engineering Dept/CEPP, Faculty of Chemical & Natural Resources Engineering, 81310 UTM Skudai, Johor Page 2. Program Objectives ...

Research paper thumbnail of Glycosylation of an immunoglobulin produced from a murine hybridoma cell line: The effect of culture mode and the anti‐apoptotic gene, bcl‐2

Biotechnology and Bioengineering, May 1, 2007

Research paper thumbnail of Mammalian Cell Culture Technology

Page 1. faam copyright (C) Mammalian Cell Culture Technology Dr Fadzilah Adibah Abdul Majid Biopr... more Page 1. faam copyright (C) Mammalian Cell Culture Technology Dr Fadzilah Adibah Abdul Majid Bioprocess Engineering Department, FKKKSA, UTM, 81310 Skudai, Johor. adibah@fkkksa.utm. my Page 2. faam copyright (C) •Introduction •Techniques in cell culture technology ...

Research paper thumbnail of The effect of bcl-2 over-expression on hybridoma cell line cultured in different serum free media

... Fadzilah Adibah Abdul Majid'and Bioprocess Engineering Dept, Faculty of Chemical Enginee... more ... Fadzilah Adibah Abdul Majid'and Bioprocess Engineering Dept, Faculty of Chemical Engineering and Natural Resources Engineering, Universiti Teknotogi Malaysia, 81310 Skudai, Johor, Malaysia. Email: adi bahfiifkkksa.utm.mv (corresponding author) Chemical Engineering ...

Research paper thumbnail of The Mechanisms of Inhibition of Advanced Glycation End Products Formation through Polyphenols in Hyperglycemic Condition

Planta medica, Jan 9, 2015

Glycation, the non-enzymatic binding of glucose to free amino groups of an amino acid, yields irr... more Glycation, the non-enzymatic binding of glucose to free amino groups of an amino acid, yields irreversible heterogeneous compounds known as advanced glycation end products. Those products play a significant role in diabetic complications. In the present article we briefly discuss the contribution of advanced glycation end products to the pathogenesis of diabetic complications, such as atherosclerosis, diabetic retinopathy, nephropathy, neuropathy, and wound healing. Then we mention the various mechanisms by which polyphenols inhibit the formation of advanced glycation end products. Finally, recent supporting documents are presented to clarify the inhibitory effects of polyphenols on the formation of advanced glycation end products. Phytochemicals apply several antiglycation mechanisms, including glucose metabolism, amelioration of oxidative stress, scavenging of dicarbonyl species, and up/down-regulation of gene expression. To utilize polyphenols in order to remedy diabetic complica...

Research paper thumbnail of Assessment of Potential Toxicological Effects of Cinnamon Bark Aqueous Extract in Rats

International Journal of Bioscience, Biochemistry and Bioinformatics, 2015

ABSTRACT Cinnamon stick is world widely used in cooking, traditional medicine, perfumery and aest... more ABSTRACT Cinnamon stick is world widely used in cooking, traditional medicine, perfumery and aesthetic industries. Many studies have demonstrated the potential of cinnamon extracts in diabetes treatment. Although it has been reported as safe in general cooking recipe and categorized as GRAS by USDA, sub-acute toxicity procedure was conducted in this study to determine effect of cinnamon extract on histopathological changes as well as the haematological parameters of blood. Water extraction was done for dried cinnamon. Twenty-four female Sprague Dawley rats were used in this study. The oral route was selected because it is the most likely route of human exposure through the consumption of herbs. The concentrations studied were 0.1, 0.5 and 2.0g/kg cinnamon aqueous extract (CE). There were no statistically significant effects of all concentrations of CE on behaviour, mortality, water intake, food consumption, weight gain, internal organs weight (liver and kidney) and heamatological parameters during treatment and post-treatment periods except 1) the slight decrease in kidney and liver weight of rats treated with 0.5g/kg and 2) slight decrease in liver weight of rats treated with 2.0g/kg, during post-treatment period. Hence, these toxicity studies suggest that the CE is low to moderate in toxicity and CE below 0.5 g/kg dose level is safe to be used in the efficacy study especially for diabetes treatment.

Research paper thumbnail of Human Papilloma Virus E6/E7 Messenger Rna as a Biomarker for Detecting the Risk Evaluation of Cervical Cancer Progression: Overview of Recent Clinical Trials

Research paper thumbnail of Wax-Impregnated Cotton Fabrics as Cell Culture Platform

Advanced Materials Research, 2015

Research paper thumbnail of In Vitro Antiproliferative Activity of Fresh Pineapple Juices on Ovarian and Colon Cancer Cell Lines

International Journal of Peptide Research and Therapeutics, 2015

Research paper thumbnail of 2) Patent Application: “Cloth-based Microfluidic Devices (CMDs) by Master Stamp”

Research paper thumbnail of The Performance of Different PreTreatment Techniques in the Isolation and Purification of Monoclonal IgG Antibody

In this study, the effects of different sample preparation techniques on the separation of monocl... more In this study, the effects of different sample preparation techniques on the separation of monoclonal antibody IgG1 were investigated experimentally. Monoclonal IgG1 was obtained from hybridoma cell line TB/C3 transfected with bcl-2 carrier plasmid, which was grown in serum-free medium. Three different pre-treatment techniques prior to Protein G affinity chromatography have been used in order to concentrate and partial purify the monoclonal antibody. The pre-treatments researched in this paper are precipitation of the antibody by ammonium sulfate, dilution of the antibody in the binding buffer of affinity chromatography and ultrafiltration through an Amicon Ultra-15 filter with molecular weight cut-off at 100 kDa. Purification through direct application of the antibody onto the Protein G affinity column without pre-treatments was used as a control method. The results indicate that the ultrafiltration through an Amicon filter was an effective method for both concentration and partial...

Research paper thumbnail of Cinnamtannin B1 activity on adipocytes formation

The Medical journal of Malaysia, 2004

In attempt to discover a small active compound that could promote adipogenesis, we investigated t... more In attempt to discover a small active compound that could promote adipogenesis, we investigated the ability of cinnamon (Cinnamomum zeylanicum) extracts to stimulate 3T3-L1 preadipocytes, In this study, we designed an experiment by replacing insulin with cinnamon extracts. The differentiated of 3T3-L1 adipocytes were monitored using oil red O staining method. Induction of adipocyte formation by cinnamtannin B1 or water extract gave the similar effects to insulin activity in adipogenesis.

Research paper thumbnail of Cloth-Based Microfluidic Devices (CMDs)

Research paper thumbnail of Cloth as a Flexible Platform for Low-Cost Microfluidic Colorimetric Assay

Research paper thumbnail of The impact of BCL-2 overexpression on product mab glycosylation hybridoma cultures

Research paper thumbnail of The physiology of the Skin

Page 1. The physiology of the Skin By Dr Fadzilah Adibah Abdul Majid A Three Day Hands-On Course ... more Page 1. The physiology of the Skin By Dr Fadzilah Adibah Abdul Majid A Three Day Hands-On Course on Household and Personal Care Product Formulations 17-19 May 2004 CEPP, UTM Page 2. The skin is a window through which the physician can "see" the entire body ...

Research paper thumbnail of Educating Engineers: Contribution Of Muslim Women EducatorsIn Universiti Teknologi Malaysia

Research paper thumbnail of Anti-Inflammatory Activity of Selected Edible Herbs and Spices on Cultured Human Gingival Fibroblasts

The Open Conference Proceedings Journal, 2013

Research paper thumbnail of Degradation and in vitro cell–material interaction studies on hydroxyapatite-coated biodegradable porous iron for hard tissue scaffolds

Journal of Orthopaedic Translation, 2014

Research paper thumbnail of Anti-Bacterial Activity of Cinnamon Oil on Oral Pathogens

The Open Conference Proceedings Journal, 2013

Research paper thumbnail of Pineapple Branding and Biorefinery Program(PBBP)

Page 1. Pineapple Branding and Biorefinery Program (PBBP) Fadzilah Adibah bt Hj. Abdul Majid, PhD... more Page 1. Pineapple Branding and Biorefinery Program (PBBP) Fadzilah Adibah bt Hj. Abdul Majid, PhD Bioprocess Engineering Dept/CEPP, Faculty of Chemical & Natural Resources Engineering, 81310 UTM Skudai, Johor Page 2. Program Objectives ...

Research paper thumbnail of Glycosylation of an immunoglobulin produced from a murine hybridoma cell line: The effect of culture mode and the anti‐apoptotic gene, bcl‐2

Biotechnology and Bioengineering, May 1, 2007

Research paper thumbnail of Mammalian Cell Culture Technology

Page 1. faam copyright (C) Mammalian Cell Culture Technology Dr Fadzilah Adibah Abdul Majid Biopr... more Page 1. faam copyright (C) Mammalian Cell Culture Technology Dr Fadzilah Adibah Abdul Majid Bioprocess Engineering Department, FKKKSA, UTM, 81310 Skudai, Johor. adibah@fkkksa.utm. my Page 2. faam copyright (C) •Introduction •Techniques in cell culture technology ...

Research paper thumbnail of The effect of bcl-2 over-expression on hybridoma cell line cultured in different serum free media

... Fadzilah Adibah Abdul Majid'and Bioprocess Engineering Dept, Faculty of Chemical Enginee... more ... Fadzilah Adibah Abdul Majid'and Bioprocess Engineering Dept, Faculty of Chemical Engineering and Natural Resources Engineering, Universiti Teknotogi Malaysia, 81310 Skudai, Johor, Malaysia. Email: adi bahfiifkkksa.utm.mv (corresponding author) Chemical Engineering ...

Research paper thumbnail of The Mechanisms of Inhibition of Advanced Glycation End Products Formation through Polyphenols in Hyperglycemic Condition

Planta medica, Jan 9, 2015

Glycation, the non-enzymatic binding of glucose to free amino groups of an amino acid, yields irr... more Glycation, the non-enzymatic binding of glucose to free amino groups of an amino acid, yields irreversible heterogeneous compounds known as advanced glycation end products. Those products play a significant role in diabetic complications. In the present article we briefly discuss the contribution of advanced glycation end products to the pathogenesis of diabetic complications, such as atherosclerosis, diabetic retinopathy, nephropathy, neuropathy, and wound healing. Then we mention the various mechanisms by which polyphenols inhibit the formation of advanced glycation end products. Finally, recent supporting documents are presented to clarify the inhibitory effects of polyphenols on the formation of advanced glycation end products. Phytochemicals apply several antiglycation mechanisms, including glucose metabolism, amelioration of oxidative stress, scavenging of dicarbonyl species, and up/down-regulation of gene expression. To utilize polyphenols in order to remedy diabetic complica...

Research paper thumbnail of Assessment of Potential Toxicological Effects of Cinnamon Bark Aqueous Extract in Rats

International Journal of Bioscience, Biochemistry and Bioinformatics, 2015

ABSTRACT Cinnamon stick is world widely used in cooking, traditional medicine, perfumery and aest... more ABSTRACT Cinnamon stick is world widely used in cooking, traditional medicine, perfumery and aesthetic industries. Many studies have demonstrated the potential of cinnamon extracts in diabetes treatment. Although it has been reported as safe in general cooking recipe and categorized as GRAS by USDA, sub-acute toxicity procedure was conducted in this study to determine effect of cinnamon extract on histopathological changes as well as the haematological parameters of blood. Water extraction was done for dried cinnamon. Twenty-four female Sprague Dawley rats were used in this study. The oral route was selected because it is the most likely route of human exposure through the consumption of herbs. The concentrations studied were 0.1, 0.5 and 2.0g/kg cinnamon aqueous extract (CE). There were no statistically significant effects of all concentrations of CE on behaviour, mortality, water intake, food consumption, weight gain, internal organs weight (liver and kidney) and heamatological parameters during treatment and post-treatment periods except 1) the slight decrease in kidney and liver weight of rats treated with 0.5g/kg and 2) slight decrease in liver weight of rats treated with 2.0g/kg, during post-treatment period. Hence, these toxicity studies suggest that the CE is low to moderate in toxicity and CE below 0.5 g/kg dose level is safe to be used in the efficacy study especially for diabetes treatment.

Research paper thumbnail of Human Papilloma Virus E6/E7 Messenger Rna as a Biomarker for Detecting the Risk Evaluation of Cervical Cancer Progression: Overview of Recent Clinical Trials

Research paper thumbnail of Wax-Impregnated Cotton Fabrics as Cell Culture Platform

Advanced Materials Research, 2015

Research paper thumbnail of In Vitro Antiproliferative Activity of Fresh Pineapple Juices on Ovarian and Colon Cancer Cell Lines

International Journal of Peptide Research and Therapeutics, 2015

Research paper thumbnail of 2) Patent Application: “Cloth-based Microfluidic Devices (CMDs) by Master Stamp”

Research paper thumbnail of The Performance of Different PreTreatment Techniques in the Isolation and Purification of Monoclonal IgG Antibody

In this study, the effects of different sample preparation techniques on the separation of monocl... more In this study, the effects of different sample preparation techniques on the separation of monoclonal antibody IgG1 were investigated experimentally. Monoclonal IgG1 was obtained from hybridoma cell line TB/C3 transfected with bcl-2 carrier plasmid, which was grown in serum-free medium. Three different pre-treatment techniques prior to Protein G affinity chromatography have been used in order to concentrate and partial purify the monoclonal antibody. The pre-treatments researched in this paper are precipitation of the antibody by ammonium sulfate, dilution of the antibody in the binding buffer of affinity chromatography and ultrafiltration through an Amicon Ultra-15 filter with molecular weight cut-off at 100 kDa. Purification through direct application of the antibody onto the Protein G affinity column without pre-treatments was used as a control method. The results indicate that the ultrafiltration through an Amicon filter was an effective method for both concentration and partial...

Research paper thumbnail of Cinnamtannin B1 activity on adipocytes formation

The Medical journal of Malaysia, 2004

In attempt to discover a small active compound that could promote adipogenesis, we investigated t... more In attempt to discover a small active compound that could promote adipogenesis, we investigated the ability of cinnamon (Cinnamomum zeylanicum) extracts to stimulate 3T3-L1 preadipocytes, In this study, we designed an experiment by replacing insulin with cinnamon extracts. The differentiated of 3T3-L1 adipocytes were monitored using oil red O staining method. Induction of adipocyte formation by cinnamtannin B1 or water extract gave the similar effects to insulin activity in adipogenesis.

Research paper thumbnail of Cloth-Based Microfluidic Devices (CMDs)

Research paper thumbnail of Cloth as a Flexible Platform for Low-Cost Microfluidic Colorimetric Assay

Research paper thumbnail of The Role of Active Compounds in Honey-iQfood Herbal Supplement for Brain Health

Honey-iQfood is an herbal supplement for brain health. It is made of a mixture of polyherbal extr... more Honey-iQfood is an herbal supplement for brain health. It is made of a mixture of polyherbal extracts and wild honey. The mixture is traditionally claimed to boost memory function, reduced or prevent dementia, Alzheimer, Parkinson, increase mental alertness and improve various conditions related to brain cells and brain functions. This formula is also specialized for kids to excel in memorizing, increase mental alertness, improve brain activity of people with Down syndrome and slow IQ. This article reports for the first time the rational roles of Honey-iQfood for brain function based on the validated secondary scientific studies. This review significantly supports the possible function of Honey-iQfood based on its main phytochemical content of such as Curcumin, Gallic acid, Catechin, Rosmarinic acid and Andrographolide, which have been intensively investigated in brain related studies. Thus it is suggested that Honey-iQfood could have been effective as herbal supplement to boost the brain activities based on the presence of important phytochemicals.