Julkipli Amsyir Sayogi Parta | Institut Pertanian Bogor (IPB) (original) (raw)

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Papers by Julkipli Amsyir Sayogi Parta

Research paper thumbnail of Antibacterial Screening of Mangrove Extract Library Showed Potential Activity against Escherichia coli and Staphylococcus aureus

Journal of Tropical Life Science, 2020

Humans are at a continuous battle against different types of diseases, so that extraordinary effo... more Humans are at a continuous battle against different types of diseases, so that extraordinary effort to accelerate drug discovery has become a necessity. Indonesian biodiversity is abundant natural resources that can be utilized as potential drug sources. Mangroves are among potential plant medicines that grow nearly at all Indonesian coastlines. The aim of this study was to evaluate the potential of mangrove extracts (extract library) as antibacterial agents. In this study, eight mangroves species with 16 samples from different parts of the plants such as leaf, bark or root were collected. Four types of solvents with different polarity, namely water, ethanol, ethyl acetate and hexane were used in maceration of samples producing 64 extracts. Disk diffusion method was used for antibacterial screening using five bacterial strains. There were 37 extracts showed antibacterial potential with the lowest and the highest inhibition indexes were 0.0283 and 1.8983, respectively. The highest inhibition index was recorded for ethyl acetate extract of Bruguiera gymnorrhiza root (BgR (Ea)) against Escherichia coli. The second highest inhibition index was 0.7867 recorded water extract of Avicennia marina leaf (AmL (A)) against Staphylococcus aureus. Almost all of extract showed saponin and tannin in considerable amount. This supported the data that mangrove extracts were potential as antibacterial agents.

Research paper thumbnail of Introduction of bioprospecting opportunities for Indonesian mangrove species

IOP Conference Series: Earth and Environmental Science, 2018

Research paper thumbnail of Optimization of cephalosporin C acylase immobilization using crosslinked enzyme aggregates technique

Bionatura, 2022

Cephalosporin C acylase (CCA) is an essential enzyme for the one-step conversion of cephalosporin... more Cephalosporin C acylase (CCA) is an essential enzyme for the one-step conversion of cephalosporin C into 7-aminocephalosporanic acid (7-ACA), an intermediate compound used to synthesize various semi-synthetic cephalosporin antibiotics. The industrial process prefers to use enzymes in immobilized form rather than soluble. A crosslinked enzyme aggregate (CLEAs) is a potential matrix-less enzyme immobilization technique to produce stable immobilized enzymes with high activity and low production costs. This study aimed to optimize the CCA immobilization using the CLEAs technique with Chitosan as a co-aggregate. The CCA lysate was obtained from harvesting CCA fermentation broth using a mutant strain of Escherichia coli through cell separation and lysis steps. Partially purified CCA by ammonium sulfate addition was conducted to obtain an active fraction of 20-60% saturation, followed by co-aggregation with Chitosan to form physical CCA aggregates. The aggregates were then immobilized by a...

Research paper thumbnail of Optimization of cephalosporin C acylase immobilization using crosslinked enzyme aggregates technique

Cephalosporin C acylase (CCA) is an essential enzyme for the one-step conversion of cephalosporin... more Cephalosporin C acylase (CCA) is an essential enzyme for the one-step conversion of cephalosporin C into 7-aminocephalosporanic acid (7-ACA), an intermediate compound used to synthesize various semi-synthetic cephalosporin antibiotics. The industrial process prefers to use enzymes in immobilized form rather than soluble. A crosslinked enzyme aggregate (CLEAs) is a potential matrix-less enzyme immobilization technique to produce stable immobilized enzymes with high activity and low production costs. This study aimed to optimize the CCA immobilization using the CLEAs technique with Chitosan as a co-aggregate. The CCA lysate was obtained from harvesting CCA fermentation broth using a mutant strain of Escherichia coli through cell separation and lysis steps. Partially purified CCA by ammonium sulfate addition was conducted to obtain an active fraction of 20-60% saturation, followed by co-aggregation with Chitosan to form physical CCA aggregates. The aggregates were then immobilized by a crosslinking technique using glutaraldehyde to form CLEAs-CCA. Optimization of the immobilization process was carried out by Response Surface Methodology in three steps, (i) screening of the influencing factors, (ii) determining the level of the significant factors, and (iii) optimizing the immobilization condition. The CLEAs-CCA activity was used as a response parameter. Under optimum conditions, CLEAs-CCA activity obtained was 85.91 Ug-1 .

Research paper thumbnail of Introduction of bioprospecting opportunities for Indonesian mangrove species

Indonesia is one of the world's most biodiverse countries. This study would be focused only on th... more Indonesia is one of the world's most biodiverse countries. This study would be focused only on the Indonesian mangrove forests biodiversity. There are about three million hectares of mangrove forests that grow along the 95,000 km of Indonesian coastline. Mangrove forests have ecology, social, economic and medicinal values that have been used by people who live along coastal area for centuries. Many studies have shown that mangrove extracts contain many bioactive compounds that have the medicinal potential for a variety of diseases. However, mangrove plant extracts are yet to be commercially formulated as modern medicines. Although Indonesia is home to one of the largest biodiversity of plants, the interest of pharmaceutical industries in the development of herbal medicines as drugs are not as promising as those from chemical synthetics. One of the reasons for this phenomenon is a low interest in synthesizing bulks of natural products. In addition, there is a lack of facilities which can provide optimization of the herbal materials. The aim of this article is to give a rational approach for designing a bioprospecting program as an initiation on the primary screening of novel drugs from Indonesian mangrove species.

Research paper thumbnail of Exploration of antibacterial activity of mangrove bruguiera cylindrica leaf extracts

Antibacterial drugs derived from natural sources play significant roles in the prevention and tre... more Antibacterial drugs derived from natural sources play significant roles in the prevention and treatment of bacterial infections since antibiotics have become less effective against many infectious diseases. Mangroves are very potential natural antibacterial sources among great numbers of wild medicinal plants. Bruguiera cylindrica is one of the many mangroves species which spread along Indonesian coastline. The aim of this study was to explore the antibacterial activity of B. cylindrica wet and dried leaf extracts. The wet extracts study was conducted with three different solvents system (water, ethanol, and n-Hexane) against Escherichia coli and Staphylococcus aureus. While, the dried extracts study was conducted with four different solvents system (water, ethanol, chloroform and n-Hexane) against three types of bacteria, Escherichia coli, Staphylococcus epidermidis and Staphylococcus aureus. The study showed that ethanol was the best solvent for extraction of phenolic and flavonoid. Antibacterial actitivity was measured by zone of inhibition which obtained from agar-disk diffusion method. The widest area of zone of inhibition was showed by wet extracts with ethanol against S. aureus and E. coli are 14.30 and 13.30 mm, respectively. While, the zone of inhibition dried extracts with ethanol against S. aureus, S. epidermidis and E. coli are 9.32, 6.59 and 6.20 mm, respectively. In conclusion, both type of extracts showed significant antibacterial activity against gram-positive bacteria as crude extracts.

Research paper thumbnail of Antibacterial Screening of Mangrove Extract Library Showed Potential Activity against Escherichia coli and Staphylococcus aureus

Journal of Tropical Life Science, 2020

Humans are at a continuous battle against different types of diseases, so that extraordinary effo... more Humans are at a continuous battle against different types of diseases, so that extraordinary effort to accelerate drug discovery has become a necessity. Indonesian biodiversity is abundant natural resources that can be utilized as potential drug sources. Mangroves are among potential plant medicines that grow nearly at all Indonesian coastlines. The aim of this study was to evaluate the potential of mangrove extracts (extract library) as antibacterial agents. In this study, eight mangroves species with 16 samples from different parts of the plants such as leaf, bark or root were collected. Four types of solvents with different polarity, namely water, ethanol, ethyl acetate and hexane were used in maceration of samples producing 64 extracts. Disk diffusion method was used for antibacterial screening using five bacterial strains. There were 37 extracts showed antibacterial potential with the lowest and the highest inhibition indexes were 0.0283 and 1.8983, respectively. The highest inhibition index was recorded for ethyl acetate extract of Bruguiera gymnorrhiza root (BgR (Ea)) against Escherichia coli. The second highest inhibition index was 0.7867 recorded water extract of Avicennia marina leaf (AmL (A)) against Staphylococcus aureus. Almost all of extract showed saponin and tannin in considerable amount. This supported the data that mangrove extracts were potential as antibacterial agents.

Research paper thumbnail of Introduction of bioprospecting opportunities for Indonesian mangrove species

IOP Conference Series: Earth and Environmental Science, 2018

Research paper thumbnail of Optimization of cephalosporin C acylase immobilization using crosslinked enzyme aggregates technique

Bionatura, 2022

Cephalosporin C acylase (CCA) is an essential enzyme for the one-step conversion of cephalosporin... more Cephalosporin C acylase (CCA) is an essential enzyme for the one-step conversion of cephalosporin C into 7-aminocephalosporanic acid (7-ACA), an intermediate compound used to synthesize various semi-synthetic cephalosporin antibiotics. The industrial process prefers to use enzymes in immobilized form rather than soluble. A crosslinked enzyme aggregate (CLEAs) is a potential matrix-less enzyme immobilization technique to produce stable immobilized enzymes with high activity and low production costs. This study aimed to optimize the CCA immobilization using the CLEAs technique with Chitosan as a co-aggregate. The CCA lysate was obtained from harvesting CCA fermentation broth using a mutant strain of Escherichia coli through cell separation and lysis steps. Partially purified CCA by ammonium sulfate addition was conducted to obtain an active fraction of 20-60% saturation, followed by co-aggregation with Chitosan to form physical CCA aggregates. The aggregates were then immobilized by a...

Research paper thumbnail of Optimization of cephalosporin C acylase immobilization using crosslinked enzyme aggregates technique

Cephalosporin C acylase (CCA) is an essential enzyme for the one-step conversion of cephalosporin... more Cephalosporin C acylase (CCA) is an essential enzyme for the one-step conversion of cephalosporin C into 7-aminocephalosporanic acid (7-ACA), an intermediate compound used to synthesize various semi-synthetic cephalosporin antibiotics. The industrial process prefers to use enzymes in immobilized form rather than soluble. A crosslinked enzyme aggregate (CLEAs) is a potential matrix-less enzyme immobilization technique to produce stable immobilized enzymes with high activity and low production costs. This study aimed to optimize the CCA immobilization using the CLEAs technique with Chitosan as a co-aggregate. The CCA lysate was obtained from harvesting CCA fermentation broth using a mutant strain of Escherichia coli through cell separation and lysis steps. Partially purified CCA by ammonium sulfate addition was conducted to obtain an active fraction of 20-60% saturation, followed by co-aggregation with Chitosan to form physical CCA aggregates. The aggregates were then immobilized by a crosslinking technique using glutaraldehyde to form CLEAs-CCA. Optimization of the immobilization process was carried out by Response Surface Methodology in three steps, (i) screening of the influencing factors, (ii) determining the level of the significant factors, and (iii) optimizing the immobilization condition. The CLEAs-CCA activity was used as a response parameter. Under optimum conditions, CLEAs-CCA activity obtained was 85.91 Ug-1 .

Research paper thumbnail of Introduction of bioprospecting opportunities for Indonesian mangrove species

Indonesia is one of the world's most biodiverse countries. This study would be focused only on th... more Indonesia is one of the world's most biodiverse countries. This study would be focused only on the Indonesian mangrove forests biodiversity. There are about three million hectares of mangrove forests that grow along the 95,000 km of Indonesian coastline. Mangrove forests have ecology, social, economic and medicinal values that have been used by people who live along coastal area for centuries. Many studies have shown that mangrove extracts contain many bioactive compounds that have the medicinal potential for a variety of diseases. However, mangrove plant extracts are yet to be commercially formulated as modern medicines. Although Indonesia is home to one of the largest biodiversity of plants, the interest of pharmaceutical industries in the development of herbal medicines as drugs are not as promising as those from chemical synthetics. One of the reasons for this phenomenon is a low interest in synthesizing bulks of natural products. In addition, there is a lack of facilities which can provide optimization of the herbal materials. The aim of this article is to give a rational approach for designing a bioprospecting program as an initiation on the primary screening of novel drugs from Indonesian mangrove species.

Research paper thumbnail of Exploration of antibacterial activity of mangrove bruguiera cylindrica leaf extracts

Antibacterial drugs derived from natural sources play significant roles in the prevention and tre... more Antibacterial drugs derived from natural sources play significant roles in the prevention and treatment of bacterial infections since antibiotics have become less effective against many infectious diseases. Mangroves are very potential natural antibacterial sources among great numbers of wild medicinal plants. Bruguiera cylindrica is one of the many mangroves species which spread along Indonesian coastline. The aim of this study was to explore the antibacterial activity of B. cylindrica wet and dried leaf extracts. The wet extracts study was conducted with three different solvents system (water, ethanol, and n-Hexane) against Escherichia coli and Staphylococcus aureus. While, the dried extracts study was conducted with four different solvents system (water, ethanol, chloroform and n-Hexane) against three types of bacteria, Escherichia coli, Staphylococcus epidermidis and Staphylococcus aureus. The study showed that ethanol was the best solvent for extraction of phenolic and flavonoid. Antibacterial actitivity was measured by zone of inhibition which obtained from agar-disk diffusion method. The widest area of zone of inhibition was showed by wet extracts with ethanol against S. aureus and E. coli are 14.30 and 13.30 mm, respectively. While, the zone of inhibition dried extracts with ethanol against S. aureus, S. epidermidis and E. coli are 9.32, 6.59 and 6.20 mm, respectively. In conclusion, both type of extracts showed significant antibacterial activity against gram-positive bacteria as crude extracts.