irma suparto | Institut Pertanian Bogor (IPB) (original) (raw)
Papers by irma suparto
Veterinary World, Nov 22, 2018
Background and Aim: This study was designed to determine the effects of a new atherogenic diet fo... more Background and Aim: This study was designed to determine the effects of a new atherogenic diet formulated at Institut Pertanian Bogor (IPB) (Bogor, Indonesia) on metabolic, morphometric, and carotid artery imaging of cynomolgus monkeys. Materials and Methods: A total of 20 adult male cynomolgus monkeys fed IPB-1 atherogenic diet for 1 year. Total plasma cholesterol (TPC), high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, triglycerides, and morphometric measurements were evaluated at baseline and monthly during the study. Carotid plaques and intima-media thickness (IMT) were measured using ultrasonography at baseline and after 8 months of treatment. Results: This diet increased TPC, LDL, and TPC/HDL ratio and induced carotid atherosclerosis in this model. The TPC, LDL, and TPC/HDL ratio were positively associated; however, HDL was negatively associated with carotid plaques and IMT. Conclusion: The IPB-1 atherogenic diet formulated with locally and...
TiAl alloys is commonly used for bone implant applications because it has good biocompatibility. ... more TiAl alloys is commonly used for bone implant applications because it has good biocompatibility. However, in long term usage of metal implants, metal release, which can harm the body, may occured. The metal release can be inhibited by hydroxyapatite-chitosan composite coating on the metal surface using Electrophoretic Deposition (EPD) method. EPD method carried out with 200 v for 40 and 60 minutes, to compare the impact of different interval time in producing the best product. The coated alloy was observed physically by SEM and showed that 60 minute-treatment produced better result. The XRD and FTIR analysis shows that the composite of hydroxyapatite-chitosan has successfully coated on TiAl alloy. The coated TiAl alloy also has low corrosion rate and could potentially be used as bone implants.
Indonesian Journal of Chemistry
The leaves of Annona muricata (sirsak), Gynura procumbens (sambung nyawa), and Typhonium flagelli... more The leaves of Annona muricata (sirsak), Gynura procumbens (sambung nyawa), and Typhonium flagelliforme (keladi tikus) have been used as traditional medicines in Indonesia. This study aims to determine the antioxidant capacity and putatively identified phenolics from the leaves of three medicinal plants forementioned above. We used the DPPH (2,2-diphenyl-1-picrylhydrazyl) method for measuring radical scavenging (antioxidant assay) while the phenolics profiling was determined using UHPLC-Q-Orbitrap HRMS. The results showed that the percentage of radical scavenging activity of G. procumbens leaves extract in ethanol was higher than the other two plants. Phenolics profiling of the three medicinal plants was identified with 38 compounds belonging to flavones and flavanols hydroxycinnamic acid, and several other groups. The number of metabolites identified putatively was 12, 31, and 19 metabolites in the extracts of A. muricata, G. procumbens, and T. flagelliforme, respectively. The resul...
Jurnal Penelitian Hasil Hutan
Jurnal Jamu Indonesia
Bahan alam yang dapat menghambat spermatogenesis merupakan suatu alternatif untuk kontrasepsi pri... more Bahan alam yang dapat menghambat spermatogenesis merupakan suatu alternatif untuk kontrasepsi pria. Penelitian ini bertujuan mengevaluasi formulasi optimum berbasis biji jarak pagar dan buah pare yang dapat menginhibisi spermatogenesis. Bahan sampel dimaserasi alkohol 70% untuk memperoleh ekstrak kasar. Ekstrak diujikan pada tikus Wistar jantan dewasa sebanyak 30 ekor dan dibagi menjadi enam kelompok secara acak (n=5 ekor). Kelompok I sebagai kontrol memperoleh pelarut akuades; kelompok II dan III secara berurutan memperoleh ekstrak biji jarak dan pare dengan dosis 50 mg/kgBB. Tiga kelompok lainnya, yaitu IV, V, dan VI memperoleh formulasi gabungan dengan rasio ekstrak biji jarak pagar dan pare 1:3, 3:1 serta 1:1, secara berurutan. Perlakuan diberikan per oral satu hari sekali selama 48 hari sesuai dengan siklus spermatogenesis. Pada akhir perlakuan, pemeriksaan sperma untuk konsentrasi dan kualitasnya serta bobot testis. Hasil uji rendemen ekstrak biji jarak pagar dan buah pare sec...
Jurnal Jamu Indonesia
Cajuput is one of plants containing essential oil with cineole as a major component. This aim of ... more Cajuput is one of plants containing essential oil with cineole as a major component. This aim of study is to separate cineole in cajuput oil and to analyze its potency as slimming aromatherapy through in vivo assay. The essential oil was fractionated by column chromatography resulting 23 fraction (F1-F23). Cajuput oil, cineole, and F9 were analyzed by gas chromatograph-mass spectrometer, and the slimming aromatherapy potency was studied on white adult male Sprague-Dawley rats. Inhalation result of cineole showed that the average body weight of rats after 5 weeks treatment period was lower than that of the normal and the control groups which consumed high cholesterol feed. In conclusion, cineole is a compound that is potential in slimming aromatherapy.
Journal of the Indonesian Chemical Society
Zingiberaceae is a family of plant that has been widely used to treat various diseases and as an ... more Zingiberaceae is a family of plant that has been widely used to treat various diseases and as an element of spice in cooking. In this paper, the potential of the extract from the steam distillation residue of Zingiberaceae leaves as antibacterial and biofilm degradation agent was studied and determined against Streptococcus mutans. Five different species of Zingiberaceae, which consisted of Curcuma longa, Curcuma zeodoaria, Curcuma aeruginosa, Zingiber officinale, Zingiber cassumunar were taken for samples and their distillation residues were extracted by soxhlation using 3 different solvents namely n-hexane, ethyl acetate, and methanol. The antibacterial and biofilm degradation activity of the assay from each of the samples was determined by the microdilution technique. Among the 15 Zingiberaceae leaves distillation residue extracts, five are categorically active against Streptococcus mutans with their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (M...
HAYATI Journal of Biosciences
IOP Conference Series: Materials Science and Engineering
Acta VETERINARIA Indonesiana
Nikotin dengan dosis tertentu telah dilaporkan memiliki pengaruh terhadap penurunan nafsu makan p... more Nikotin dengan dosis tertentu telah dilaporkan memiliki pengaruh terhadap penurunan nafsu makan pada tikus, tetapi belum pernah dilaporkan pada hewan model monyet ekor panjang. Oleh karena itu penelitian ini bertujuan untuk mengetahui pengaruh nikotin terhadap bobot badan, indeks massa tubuh (IMT) dan kadar glukosa darah monyet ekor panjang (Macaca fascicularis) yang diberi diet berenergi tinggi selama tiga bulan. Sepuluh monyet jantan dewasa secara acak dibagi menjadi dua kelompok. Kelompok pertama diberi pakan dengan bahan utama dari lemak sapi dan kelompok kedua dengan pakan monyet komersial (monkey chow). Cairan nikotin ditambahkan kedua pakan dengan dosis 0,75 mg/kg berat badan. Rancangan yang digunakan dalam penelitian ini adalah rancangan acak lengkap (tersarang) dalam waktu. Data yang dikumpulkan dianalisis untuk mengetahui hubungan antara waktu dan perlakuan. Pengukuran dilakukan setiap empat minggu terhadap berat badan, indeks massa tubuh dan kadar glukosa darah. Hasil pen...
Acta VETERINARIA Indonesiana
Aterosklerosis pada arteri iliak berkaitan dengan kejadian peripheral arterial disease (PAD) pada... more Aterosklerosis pada arteri iliak berkaitan dengan kejadian peripheral arterial disease (PAD) pada manusia. Penelitian ini bertujuan untuk menganalisis lesio aterosklerosis pada arteri iliak sebagai salah satu faktor penyebab PAD. Penelitian ini menggunakan sembilan monyet ekor panjang jantan dewasa dari 3 kelompok respons yang diberi pakan aterogenik IPB-1 selama 2 tahun. Arteri common iliak diambil pada saat nekropsi, diproses menjadi slaid histologi dan diwarnai dengan Verhoeff van Giesson (VVG). Keparahan lesio dikategorikan sebagai grade I sampai VI dan dianalisis secara deskriptif. Area lesio ditentukan melalui panjang lamina elastik interna (PLEI), area intima (AI), serta ketebalan maksimum intima (KIM) dan dianalisis dengan ANOVA. Hasil penelitian menunjukkan 55,56% monyet membentuk lesio plak aterosklerosis (grade IV sampai VI) yang mana mereka berasal dari kelompok intermediet dan hiper-responsif. Kelompok hiper-responsif menunjukkan AI dan KMI paling tinggi (0,979 mm2; 0,3...
Acta VETERINARIA Indonesiana
An experiment to compare age of Macaca fascicularis (Mf) as pheripheral blood (PB) mesenchymal st... more An experiment to compare age of Macaca fascicularis (Mf) as pheripheral blood (PB) mesenchymal stem cell (MSC) isolate sources and the impact of its concentration on the pheriperal blood mononucleous cells (PBMC) development has been conducted. Twelve male Mf were used in this experiment. Three different age groups (infant (A1), juvenil (A2) and adult (A3)) of the Mfs were compared as treatments. Isolate of pheriperal blood MSC were created by taking 1 ml, 5 ml or 10 ml the Mfs pheriperal blood, processed them into PBMC, counted, isolated, cultured, subcultured, pelleted, extracted for their messenger Ribonucleic Acid (mRNA). Reverse transcriptase - polymerase chain reaction (RT-PCR) were conducted to obtain complentary Deoxyribonucleic Acid (cDNA). PCR amplification were performed to look cluster differentiation (CD) of the MSC gene expression. Incomplete block design was used and the data were analysed using descriptive statistic and T-Test. The results showed that PBMC counted fr...
International Neurourology Journal
Veterinary World
Background and Aim: This study was designed to determine the effects of a new atherogenic diet fo... more Background and Aim: This study was designed to determine the effects of a new atherogenic diet formulated at Institut Pertanian Bogor (IPB) (Bogor, Indonesia) on metabolic, morphometric, and carotid artery imaging of cynomolgus monkeys. Materials and Methods: A total of 20 adult male cynomolgus monkeys fed IPB-1 atherogenic diet for 1 year. Total plasma cholesterol (TPC), high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, triglycerides, and morphometric measurements were evaluated at baseline and monthly during the study. Carotid plaques and intima-media thickness (IMT) were measured using ultrasonography at baseline and after 8 months of treatment. Results: This diet increased TPC, LDL, and TPC/HDL ratio and induced carotid atherosclerosis in this model. The TPC, LDL, and TPC/HDL ratio were positively associated; however, HDL was negatively associated with carotid plaques and IMT. Conclusion: The IPB-1 atherogenic diet formulated with locally and...
Jurnal Teknologi dan Industri Pangan
E-Journal Menara Perkebunan
In plant tissue culture, the culture vessels are usuallycovered tightly with screw caps, aluminiu... more In plant tissue culture, the culture vessels are usuallycovered tightly with screw caps, aluminium foils, parafilm,or plastic wrap. This condition restricts the exchange ofgases in culture vessels and will affect negatively thegrowth of explants. The use of ventilated closure improvesthe air quality in culture vessels. Microboxes provided withdifferent types of filter (yellow filter with Kv value13.09 Gas Exchange (GE)/day, green filter with Kv value81.35 GE/day and without filter) on their closure wereexamined as culture vessels for growing rubber explants atmultiplication step. The purpose of this research was toobserve the plant condition in different types of microboxcorresponding to the morphology, stomata and chlorophyllcontent of the shoots. The results showed no significantdifference of shoot height on each microbox. The use ofventilated closure increased significantly new leafformation and decreased leaf fall. Normal size and color ofleaves were found on shoots grown in mic...
Pakistan Journal of Nutrition, 2017
Asian Pacific Journal of Tropical Biomedicine, 2017
Veterinary World, Nov 22, 2018
Background and Aim: This study was designed to determine the effects of a new atherogenic diet fo... more Background and Aim: This study was designed to determine the effects of a new atherogenic diet formulated at Institut Pertanian Bogor (IPB) (Bogor, Indonesia) on metabolic, morphometric, and carotid artery imaging of cynomolgus monkeys. Materials and Methods: A total of 20 adult male cynomolgus monkeys fed IPB-1 atherogenic diet for 1 year. Total plasma cholesterol (TPC), high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, triglycerides, and morphometric measurements were evaluated at baseline and monthly during the study. Carotid plaques and intima-media thickness (IMT) were measured using ultrasonography at baseline and after 8 months of treatment. Results: This diet increased TPC, LDL, and TPC/HDL ratio and induced carotid atherosclerosis in this model. The TPC, LDL, and TPC/HDL ratio were positively associated; however, HDL was negatively associated with carotid plaques and IMT. Conclusion: The IPB-1 atherogenic diet formulated with locally and...
TiAl alloys is commonly used for bone implant applications because it has good biocompatibility. ... more TiAl alloys is commonly used for bone implant applications because it has good biocompatibility. However, in long term usage of metal implants, metal release, which can harm the body, may occured. The metal release can be inhibited by hydroxyapatite-chitosan composite coating on the metal surface using Electrophoretic Deposition (EPD) method. EPD method carried out with 200 v for 40 and 60 minutes, to compare the impact of different interval time in producing the best product. The coated alloy was observed physically by SEM and showed that 60 minute-treatment produced better result. The XRD and FTIR analysis shows that the composite of hydroxyapatite-chitosan has successfully coated on TiAl alloy. The coated TiAl alloy also has low corrosion rate and could potentially be used as bone implants.
Indonesian Journal of Chemistry
The leaves of Annona muricata (sirsak), Gynura procumbens (sambung nyawa), and Typhonium flagelli... more The leaves of Annona muricata (sirsak), Gynura procumbens (sambung nyawa), and Typhonium flagelliforme (keladi tikus) have been used as traditional medicines in Indonesia. This study aims to determine the antioxidant capacity and putatively identified phenolics from the leaves of three medicinal plants forementioned above. We used the DPPH (2,2-diphenyl-1-picrylhydrazyl) method for measuring radical scavenging (antioxidant assay) while the phenolics profiling was determined using UHPLC-Q-Orbitrap HRMS. The results showed that the percentage of radical scavenging activity of G. procumbens leaves extract in ethanol was higher than the other two plants. Phenolics profiling of the three medicinal plants was identified with 38 compounds belonging to flavones and flavanols hydroxycinnamic acid, and several other groups. The number of metabolites identified putatively was 12, 31, and 19 metabolites in the extracts of A. muricata, G. procumbens, and T. flagelliforme, respectively. The resul...
Jurnal Penelitian Hasil Hutan
Jurnal Jamu Indonesia
Bahan alam yang dapat menghambat spermatogenesis merupakan suatu alternatif untuk kontrasepsi pri... more Bahan alam yang dapat menghambat spermatogenesis merupakan suatu alternatif untuk kontrasepsi pria. Penelitian ini bertujuan mengevaluasi formulasi optimum berbasis biji jarak pagar dan buah pare yang dapat menginhibisi spermatogenesis. Bahan sampel dimaserasi alkohol 70% untuk memperoleh ekstrak kasar. Ekstrak diujikan pada tikus Wistar jantan dewasa sebanyak 30 ekor dan dibagi menjadi enam kelompok secara acak (n=5 ekor). Kelompok I sebagai kontrol memperoleh pelarut akuades; kelompok II dan III secara berurutan memperoleh ekstrak biji jarak dan pare dengan dosis 50 mg/kgBB. Tiga kelompok lainnya, yaitu IV, V, dan VI memperoleh formulasi gabungan dengan rasio ekstrak biji jarak pagar dan pare 1:3, 3:1 serta 1:1, secara berurutan. Perlakuan diberikan per oral satu hari sekali selama 48 hari sesuai dengan siklus spermatogenesis. Pada akhir perlakuan, pemeriksaan sperma untuk konsentrasi dan kualitasnya serta bobot testis. Hasil uji rendemen ekstrak biji jarak pagar dan buah pare sec...
Jurnal Jamu Indonesia
Cajuput is one of plants containing essential oil with cineole as a major component. This aim of ... more Cajuput is one of plants containing essential oil with cineole as a major component. This aim of study is to separate cineole in cajuput oil and to analyze its potency as slimming aromatherapy through in vivo assay. The essential oil was fractionated by column chromatography resulting 23 fraction (F1-F23). Cajuput oil, cineole, and F9 were analyzed by gas chromatograph-mass spectrometer, and the slimming aromatherapy potency was studied on white adult male Sprague-Dawley rats. Inhalation result of cineole showed that the average body weight of rats after 5 weeks treatment period was lower than that of the normal and the control groups which consumed high cholesterol feed. In conclusion, cineole is a compound that is potential in slimming aromatherapy.
Journal of the Indonesian Chemical Society
Zingiberaceae is a family of plant that has been widely used to treat various diseases and as an ... more Zingiberaceae is a family of plant that has been widely used to treat various diseases and as an element of spice in cooking. In this paper, the potential of the extract from the steam distillation residue of Zingiberaceae leaves as antibacterial and biofilm degradation agent was studied and determined against Streptococcus mutans. Five different species of Zingiberaceae, which consisted of Curcuma longa, Curcuma zeodoaria, Curcuma aeruginosa, Zingiber officinale, Zingiber cassumunar were taken for samples and their distillation residues were extracted by soxhlation using 3 different solvents namely n-hexane, ethyl acetate, and methanol. The antibacterial and biofilm degradation activity of the assay from each of the samples was determined by the microdilution technique. Among the 15 Zingiberaceae leaves distillation residue extracts, five are categorically active against Streptococcus mutans with their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (M...
HAYATI Journal of Biosciences
IOP Conference Series: Materials Science and Engineering
Acta VETERINARIA Indonesiana
Nikotin dengan dosis tertentu telah dilaporkan memiliki pengaruh terhadap penurunan nafsu makan p... more Nikotin dengan dosis tertentu telah dilaporkan memiliki pengaruh terhadap penurunan nafsu makan pada tikus, tetapi belum pernah dilaporkan pada hewan model monyet ekor panjang. Oleh karena itu penelitian ini bertujuan untuk mengetahui pengaruh nikotin terhadap bobot badan, indeks massa tubuh (IMT) dan kadar glukosa darah monyet ekor panjang (Macaca fascicularis) yang diberi diet berenergi tinggi selama tiga bulan. Sepuluh monyet jantan dewasa secara acak dibagi menjadi dua kelompok. Kelompok pertama diberi pakan dengan bahan utama dari lemak sapi dan kelompok kedua dengan pakan monyet komersial (monkey chow). Cairan nikotin ditambahkan kedua pakan dengan dosis 0,75 mg/kg berat badan. Rancangan yang digunakan dalam penelitian ini adalah rancangan acak lengkap (tersarang) dalam waktu. Data yang dikumpulkan dianalisis untuk mengetahui hubungan antara waktu dan perlakuan. Pengukuran dilakukan setiap empat minggu terhadap berat badan, indeks massa tubuh dan kadar glukosa darah. Hasil pen...
Acta VETERINARIA Indonesiana
Aterosklerosis pada arteri iliak berkaitan dengan kejadian peripheral arterial disease (PAD) pada... more Aterosklerosis pada arteri iliak berkaitan dengan kejadian peripheral arterial disease (PAD) pada manusia. Penelitian ini bertujuan untuk menganalisis lesio aterosklerosis pada arteri iliak sebagai salah satu faktor penyebab PAD. Penelitian ini menggunakan sembilan monyet ekor panjang jantan dewasa dari 3 kelompok respons yang diberi pakan aterogenik IPB-1 selama 2 tahun. Arteri common iliak diambil pada saat nekropsi, diproses menjadi slaid histologi dan diwarnai dengan Verhoeff van Giesson (VVG). Keparahan lesio dikategorikan sebagai grade I sampai VI dan dianalisis secara deskriptif. Area lesio ditentukan melalui panjang lamina elastik interna (PLEI), area intima (AI), serta ketebalan maksimum intima (KIM) dan dianalisis dengan ANOVA. Hasil penelitian menunjukkan 55,56% monyet membentuk lesio plak aterosklerosis (grade IV sampai VI) yang mana mereka berasal dari kelompok intermediet dan hiper-responsif. Kelompok hiper-responsif menunjukkan AI dan KMI paling tinggi (0,979 mm2; 0,3...
Acta VETERINARIA Indonesiana
An experiment to compare age of Macaca fascicularis (Mf) as pheripheral blood (PB) mesenchymal st... more An experiment to compare age of Macaca fascicularis (Mf) as pheripheral blood (PB) mesenchymal stem cell (MSC) isolate sources and the impact of its concentration on the pheriperal blood mononucleous cells (PBMC) development has been conducted. Twelve male Mf were used in this experiment. Three different age groups (infant (A1), juvenil (A2) and adult (A3)) of the Mfs were compared as treatments. Isolate of pheriperal blood MSC were created by taking 1 ml, 5 ml or 10 ml the Mfs pheriperal blood, processed them into PBMC, counted, isolated, cultured, subcultured, pelleted, extracted for their messenger Ribonucleic Acid (mRNA). Reverse transcriptase - polymerase chain reaction (RT-PCR) were conducted to obtain complentary Deoxyribonucleic Acid (cDNA). PCR amplification were performed to look cluster differentiation (CD) of the MSC gene expression. Incomplete block design was used and the data were analysed using descriptive statistic and T-Test. The results showed that PBMC counted fr...
International Neurourology Journal
Veterinary World
Background and Aim: This study was designed to determine the effects of a new atherogenic diet fo... more Background and Aim: This study was designed to determine the effects of a new atherogenic diet formulated at Institut Pertanian Bogor (IPB) (Bogor, Indonesia) on metabolic, morphometric, and carotid artery imaging of cynomolgus monkeys. Materials and Methods: A total of 20 adult male cynomolgus monkeys fed IPB-1 atherogenic diet for 1 year. Total plasma cholesterol (TPC), high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, triglycerides, and morphometric measurements were evaluated at baseline and monthly during the study. Carotid plaques and intima-media thickness (IMT) were measured using ultrasonography at baseline and after 8 months of treatment. Results: This diet increased TPC, LDL, and TPC/HDL ratio and induced carotid atherosclerosis in this model. The TPC, LDL, and TPC/HDL ratio were positively associated; however, HDL was negatively associated with carotid plaques and IMT. Conclusion: The IPB-1 atherogenic diet formulated with locally and...
Jurnal Teknologi dan Industri Pangan
E-Journal Menara Perkebunan
In plant tissue culture, the culture vessels are usuallycovered tightly with screw caps, aluminiu... more In plant tissue culture, the culture vessels are usuallycovered tightly with screw caps, aluminium foils, parafilm,or plastic wrap. This condition restricts the exchange ofgases in culture vessels and will affect negatively thegrowth of explants. The use of ventilated closure improvesthe air quality in culture vessels. Microboxes provided withdifferent types of filter (yellow filter with Kv value13.09 Gas Exchange (GE)/day, green filter with Kv value81.35 GE/day and without filter) on their closure wereexamined as culture vessels for growing rubber explants atmultiplication step. The purpose of this research was toobserve the plant condition in different types of microboxcorresponding to the morphology, stomata and chlorophyllcontent of the shoots. The results showed no significantdifference of shoot height on each microbox. The use ofventilated closure increased significantly new leafformation and decreased leaf fall. Normal size and color ofleaves were found on shoots grown in mic...
Pakistan Journal of Nutrition, 2017
Asian Pacific Journal of Tropical Biomedicine, 2017