Manjunath Hubballi | Tamil Nadu Agricultural University (original) (raw)

Uploads

Papers by Manjunath Hubballi

Research paper thumbnail of Postharvest Diseases of Cole Crops and Their Management

Postharvest Handling and Diseases of Horticultural Produce, 2021

Research paper thumbnail of First report of leaf blight of noni caused by Alternaria alternata (Fr.) Keissler

Journal of General Plant Pathology, Aug 1, 2010

A severe outbreak of leaf blight was observed in noni crop for the first time in Tamil Nadu and K... more A severe outbreak of leaf blight was observed in noni crop for the first time in Tamil Nadu and Karnataka states of India during 2008–2009. Based on pathogenicity, morphology and ribosomal DNA spacer sequences, the pathogen was identified as Alternaria alternata (Fr.) Keissler. This report is the first of Alternaria leaf blight of noni.

Research paper thumbnail of Diversity assessment of Colletotrichum species associated with chilli anthracnose and their characterization via species specific primers

Anthracnose disease caused by Colletotrichum spp. is a major constraint for chilli production and... more Anthracnose disease caused by Colletotrichum spp. is a major constraint for chilli production and storage throughout the globe. In the present study, diversity analysis and characterization of Colletotrichum species associated with the disease was carried out. Anthracnose infected chilli samples were collected from major chilli growing areas of India and twenty five isolates were selected for species identification. Pathogenicity of all the isolates was confirmed on a susceptible variety K2. The morphological characters viz., colony colour, colony topography, conidial size and shape and size of setae revealed three morphotypes. In contrast to morphological grouping, RAPD based diversity analysis clustered all isolates into two main groups and seven sub-groups. Thereafter, five different species specific primers were employed to characterize different species of Colletotrichum, of which primers specific for C. capsici, C. gloeosporioides and C. acutatum yielded positive results. The ...

Research paper thumbnail of Physiological characterisation of Colletotrichum gloeosporioides , the incitant of anthracnose disease of noni in India

Archives Of Phytopathology And Plant Protection, 2011

Ten isolates of Colletotrichum gloeosporioides were collected from different noni growing areas o... more Ten isolates of Colletotrichum gloeosporioides were collected from different noni growing areas of Tamil Nadu, Karnataka and Kerala in India and their pathogenicity was proved under glass house conditions. Effect of different pH levels, temperature, light intensity and media were tested against the growth of C. gloeosporioides under in vitro. The results indicated that the growth of C. gloeosporioides was maximum in pH range of 6.50–7.00 and temperature range of 25–30° C. Exposure of the fungus to alternate cycles of 12 h light ...

Research paper thumbnail of Effect of Environmental Conditions on Growth of Alternaria alternata Causing Leaf Blight of Noni

Abstract: Effect of different pH levels, temperature, light intensity and media were tested again... more Abstract: Effect of different pH levels, temperature, light intensity and media were tested against the growth of A. alternata under in vitro conditions. The results of experiment indicated that the growth of A. alternata was maximum in pH range of 6.00-6.50 and temperature range of 25-30 C. The exposure of the fungus to alternate cycles of 12 hour light and 12 hour darkness resulted in the maximum mycelial growth of A. alternata compared to continuous light and dark.

Research paper thumbnail of Virulence of Alternaria Alternata Infecting Noni Associated with Production of Cell Wall Degrading Enzymes

Different methods of inoculations were tried for proving Koch postulates. Among them, the pin pri... more Different methods of inoculations were tried for proving Koch postulates. Among them, the pin prick plus spore suspension spray was found be the best method used in a glasshouse. Out of the fifteen isolates of Alternaria alternata, isolate AA1 was highly virulent and AA6 was avirulent. The virulent isolate of A. alternata produced more cellulolytic (C1 and Cx) and pectinolytic (macerating enzymes, pectin methyl esterase and endo-polygalacturonase) enzymes in vitro than the avirulent one.

Research paper thumbnail of Application of plant extracts induced the changes in biochemical composition of Banana fruits

ABSTRACT Banana fruits were dipped in 10 per cent leaf extracts of Soanum torvum, Zimmu and Alliu... more ABSTRACT Banana fruits were dipped in 10 per cent leaf extracts of Soanum torvum, Zimmu and Allium alliaceum for five min and inoculated the pathogen with conidial suspensions (106/ml) of Colletotrichum musae by pin prick method of inoculation, The changes in the biochemical constituents of the inoculated fruits, inoculated fruits dipped in leaf extracts and uninoculated control are analyzed separately both in peel and pulp at 2, 4, 6, 8 and 10 days after treatment.

Research paper thumbnail of Defense responses of grapevine to Plasmopara viticola induced by Azoxystrobin and Pseudomonas fluorescens

Abstract: The induction of systemic resistance was studied to elucidate the role of azoxystrobin ... more Abstract: The induction of systemic resistance was studied to elucidate the role of azoxystrobin and Pseuclomonasfluorescens in disease management on grapevine against Plasmopara viticola. The activity of defense enzymes viz., peroxidase (PO), polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL) was found to be increased in azoxystrobin 23 SC and P. fluorescens treated grapevine seedlings compared to control.

Research paper thumbnail of Role of plant extracts in inducing the systemic acquired resistance in harvested banana against anthracnose disease

ABSTRACT Mechanism of disease suppression by plant products have proved that, the active principl... more ABSTRACT Mechanism of disease suppression by plant products have proved that, the active principles present in them and/or induce systemic resistance in host plants. In the present investigation we studied the expression of induced systemic resistance in banana fruits upon treatment with plant extracts. Banana fruits are dipped in 10 per cent of leaf extracts of Solanum torvum, Zimmu and Allium alliaceum for five minutes and inoculated with conidial suspensions (106/ml) of Colletotrichum musae by pin prick method.

Research paper thumbnail of Postharvest Diseases of Cole Crops and Their Management

Postharvest Handling and Diseases of Horticultural Produce, 2021

Research paper thumbnail of First report of leaf blight of noni caused by Alternaria alternata (Fr.) Keissler

Journal of General Plant Pathology, Aug 1, 2010

A severe outbreak of leaf blight was observed in noni crop for the first time in Tamil Nadu and K... more A severe outbreak of leaf blight was observed in noni crop for the first time in Tamil Nadu and Karnataka states of India during 2008–2009. Based on pathogenicity, morphology and ribosomal DNA spacer sequences, the pathogen was identified as Alternaria alternata (Fr.) Keissler. This report is the first of Alternaria leaf blight of noni.

Research paper thumbnail of Diversity assessment of Colletotrichum species associated with chilli anthracnose and their characterization via species specific primers

Anthracnose disease caused by Colletotrichum spp. is a major constraint for chilli production and... more Anthracnose disease caused by Colletotrichum spp. is a major constraint for chilli production and storage throughout the globe. In the present study, diversity analysis and characterization of Colletotrichum species associated with the disease was carried out. Anthracnose infected chilli samples were collected from major chilli growing areas of India and twenty five isolates were selected for species identification. Pathogenicity of all the isolates was confirmed on a susceptible variety K2. The morphological characters viz., colony colour, colony topography, conidial size and shape and size of setae revealed three morphotypes. In contrast to morphological grouping, RAPD based diversity analysis clustered all isolates into two main groups and seven sub-groups. Thereafter, five different species specific primers were employed to characterize different species of Colletotrichum, of which primers specific for C. capsici, C. gloeosporioides and C. acutatum yielded positive results. The ...

Research paper thumbnail of Physiological characterisation of Colletotrichum gloeosporioides , the incitant of anthracnose disease of noni in India

Archives Of Phytopathology And Plant Protection, 2011

Ten isolates of Colletotrichum gloeosporioides were collected from different noni growing areas o... more Ten isolates of Colletotrichum gloeosporioides were collected from different noni growing areas of Tamil Nadu, Karnataka and Kerala in India and their pathogenicity was proved under glass house conditions. Effect of different pH levels, temperature, light intensity and media were tested against the growth of C. gloeosporioides under in vitro. The results indicated that the growth of C. gloeosporioides was maximum in pH range of 6.50–7.00 and temperature range of 25–30° C. Exposure of the fungus to alternate cycles of 12 h light ...

Research paper thumbnail of Effect of Environmental Conditions on Growth of Alternaria alternata Causing Leaf Blight of Noni

Abstract: Effect of different pH levels, temperature, light intensity and media were tested again... more Abstract: Effect of different pH levels, temperature, light intensity and media were tested against the growth of A. alternata under in vitro conditions. The results of experiment indicated that the growth of A. alternata was maximum in pH range of 6.00-6.50 and temperature range of 25-30 C. The exposure of the fungus to alternate cycles of 12 hour light and 12 hour darkness resulted in the maximum mycelial growth of A. alternata compared to continuous light and dark.

Research paper thumbnail of Virulence of Alternaria Alternata Infecting Noni Associated with Production of Cell Wall Degrading Enzymes

Different methods of inoculations were tried for proving Koch postulates. Among them, the pin pri... more Different methods of inoculations were tried for proving Koch postulates. Among them, the pin prick plus spore suspension spray was found be the best method used in a glasshouse. Out of the fifteen isolates of Alternaria alternata, isolate AA1 was highly virulent and AA6 was avirulent. The virulent isolate of A. alternata produced more cellulolytic (C1 and Cx) and pectinolytic (macerating enzymes, pectin methyl esterase and endo-polygalacturonase) enzymes in vitro than the avirulent one.

Research paper thumbnail of Application of plant extracts induced the changes in biochemical composition of Banana fruits

ABSTRACT Banana fruits were dipped in 10 per cent leaf extracts of Soanum torvum, Zimmu and Alliu... more ABSTRACT Banana fruits were dipped in 10 per cent leaf extracts of Soanum torvum, Zimmu and Allium alliaceum for five min and inoculated the pathogen with conidial suspensions (106/ml) of Colletotrichum musae by pin prick method of inoculation, The changes in the biochemical constituents of the inoculated fruits, inoculated fruits dipped in leaf extracts and uninoculated control are analyzed separately both in peel and pulp at 2, 4, 6, 8 and 10 days after treatment.

Research paper thumbnail of Defense responses of grapevine to Plasmopara viticola induced by Azoxystrobin and Pseudomonas fluorescens

Abstract: The induction of systemic resistance was studied to elucidate the role of azoxystrobin ... more Abstract: The induction of systemic resistance was studied to elucidate the role of azoxystrobin and Pseuclomonasfluorescens in disease management on grapevine against Plasmopara viticola. The activity of defense enzymes viz., peroxidase (PO), polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL) was found to be increased in azoxystrobin 23 SC and P. fluorescens treated grapevine seedlings compared to control.

Research paper thumbnail of Role of plant extracts in inducing the systemic acquired resistance in harvested banana against anthracnose disease

ABSTRACT Mechanism of disease suppression by plant products have proved that, the active principl... more ABSTRACT Mechanism of disease suppression by plant products have proved that, the active principles present in them and/or induce systemic resistance in host plants. In the present investigation we studied the expression of induced systemic resistance in banana fruits upon treatment with plant extracts. Banana fruits are dipped in 10 per cent of leaf extracts of Solanum torvum, Zimmu and Allium alliaceum for five minutes and inoculated with conidial suspensions (106/ml) of Colletotrichum musae by pin prick method.