Anjana Devkota | Tribhuvan University (original) (raw)

Papers by Anjana Devkota

Middle-East Journal of Scientific Research, 2010

... 1990-9233 © IDO SI Publications, 2010 Effects of Different Light Levels on the Growth Traits ... more ... 1990-9233 © IDO SI Publications, 2010 Effects of Different Light Levels on the Growth Traits and Yield of Centella asiatica Anjana Devkota and Pramod Kumar Jha Department Central ... 9. Zobel, DB, PK Jha, MJ Behan and UKR Yadav, 1987 ... Ratna Book Distributor, Kathmandu 10 ...

Brazilian Journal of Plant Physiology, 2010

Abstract: The effect of variation in soil composition on growth vigour of Centella asiatica (L) U... more Abstract: The effect of variation in soil composition on growth vigour of Centella asiatica (L) Urb. was

Biochemical Systematics …, 2010

Centella asiatica (L.) Urban is widely used in traditional medicine in many countries and in the ... more Centella asiatica (L.) Urban is widely used in traditional medicine in many countries and in the formulation of drugs and cosmetics, and is therefore suitable as a trade item for the development of medicinal plants for the population of Nepal. The aim of this work was to select plant populations of C. asiatica with high contents of secondary metabolites growing in various localities in Nepal, and to enhance knowledge of the cultivation of this plant. Quali-quantitative analysis of bioactive triterpenes (asiaticoside and asiatic acid) and phenol derivatives (flavonoids and caffeoyl esters) was performed by HPLC-DAD-ELSD. The highest quantities of triterpenes and phenols were found in samples from the Gorkha and Chitwan districts. Regarding cultivated plants, soil fertilisation is critical, since over-rich soils affect secondary metabolite content. Plants growing in sand-rich soils produce more terpenes. This work provides indications on how to select high-terpene producing germplasm and recommendations for plant cultivation.

Additional file 1: Table S1. Total Survey sites showing total number of plants infested by Puccin... more Additional file 1: Table S1. Total Survey sites showing total number of plants infested by Puccinia abrupta var. partheniicola and rust incidence in different locations of Kathmandu Valley. Fig. S1. Photographs of Parthenium hysterophorus infested by Puccinia abrupta var. partheniicola in different severity classes (A - healthy plant with no infestation; B – only a few leaves infested without any apparent impact on growth; C – premature senescence of some leaves but not apparent impact on inflorescence; D – death of about 50% leaves with apparent impacts on inflorescence but not senescence of plant; E – death or senescence of plant) (A, D: S. Maharjan; B, C, E: BB Shrestha). Fig. S2. Microscopic image of urediniospores of Puccinia abrupta var. partheniicola stained in cotton blue

Abstract: The laboratory tests were conducted to evaluate the allelopathic potential and phytoche... more Abstract: The laboratory tests were conducted to evaluate the allelopathic potential and phytochemical study of the four selected medicinal plants Ageratum conyzoides, Eclipta prostrata, Cannabis sativa and Woodfordia fructicosa. The aqueous extract bioassay of the plants with stem and root were conducted on the two test seeds Triticum aestivum (wheat) and Pisum sativum (pea) by filter paper method. Germination, seedling growth and biomass production were observed under the allelopathic study whereas under phytochemical study qualitative or presence or absence and quantitative or crude amount of the phytochemicals present were also estimated. For the allelopathic studies the germination and seedling growth of wheat and pea test species under the different concentrations 2 %, 4%, 6%, 8 % and 10 % of the stem and root extracts of the plants under study were carried out. Increased concentration of the aqueous extracts of the plants increased the inhibitory rate or decreased the germina...

Journal of Institute of Science and Technology

The present research aims to know the regeneration status of community managed Shorea robusta (Sa... more The present research aims to know the regeneration status of community managed Shorea robusta (Sal) forests; managed for 6-21 years in Tanahun district, a part of Gandaki Province, Nepal. These forests were categorized into two groups according to management duration (more than 10 years and equal or less than 10 yrs). The regeneration status of the forest was estimated by calculating the density of each species in each developmental phase (seedling, sapling and tree). The total tree density of community forest managed for more than 10 years (MCF forest; 1230 plants/ha) was less than the community forest managed for equal or less than 10 years (LCF forest; 1314 plants/ha). The results suggest that the size class distribution of the trees resembling inverse-J shaped indicates the good regenerating capability of both forests. After the handover of forests to the community, Sal density had increased rapidly in both the forests. Community management had a significant positive impact on t...

Scientific World, 2021

The urban air pollution is a major environmental concern, particularly in the developing countrie... more The urban air pollution is a major environmental concern, particularly in the developing countries and in their major cities. In the present study an attempt has been made to assess the air pollution effect on micromorphological and biochemical parameters of Callistemon citrinus and Lagerstroemia indica. Biochemical parameters were analyzed by using standard protocol. one-way analysis of variance was performed with SPSS software, v. 20.0, and Duncan test (p ≤ 0.05) was used to compare averages of all measured biochemical parameters and micromorpholgical features. There was a decrease in number of stomata of both species at highly polluted site compared to less polluted site (control). Stomatal index of both species was found to be reduced in polluted site when compared to control. The number of clogged stomata was less in control area samples when compared to polluted sample. A number of epidermal cells were significantly decreased at heavily polluted site. Total chlorophyll content...

International Agrophysics, 2011

A b s t r a c t. The influence of water stress on growth and yield of Centella asiatica, a tradit... more A b s t r a c t. The influence of water stress on growth and yield of Centella asiatica, a traditional medicinal herb of Nepal, was carried out in a pot experiment. Variation in different growth traits of Centella asiatica was investigated using vegetative clones of one population. The plantlets were grown in earthen pots containing soil, sand and vermicompost and treated with different levels of water stress (30, 70, 100, and 125% of pot capacity by mass). The experimental design was completely randomized and each treatment was composed of forty plants. An array of vegetative traits including: number of leaves, petiole length, specific leaf area, number of primary branches, and plant biomass was examined. Growth traits such as root length, leaf area and number of flowers per ramet demonstrated significant variation in response to water stress. The results suggested that plants irrigated to 100% pot water capacity showed highest growth and plant biomass production. K e y w o r d s: ...

Indian Journal of Natural Products and Resources, 2020

Ageratum houstonianum Mill. (Asteraceae) leaves extract (in distilled water and methanol) was eva... more Ageratum houstonianum Mill. (Asteraceae) leaves extract (in distilled water and methanol) was evaluated against five phytopathogenic fungi: Alternaria brassicae, Botrytis cinerea, Fusarium oxysporum, Phytophthora capsici and Sclerotium rolfsii at different concentrations (50, 100, 150, 200, and 250 mg/mL). The phytochemical screening depicted the presence of terpenoids, saponins, flavonoids, tannins and alkaloids. The activity test of extracts against fungi was determined by poisoned food technique and linear mycelium growth reduction (LMGR) percentage was calculated. Methanol crude leaf extract had higher antifungal potential than the distilled water extract. Aqueous and methanolic extracts of leaves of A. houstonianum greatly reduced the mycelium growth of tested fungi, which can be used for the disease management.

Antifungal activities and phytochemical screening of Xanthium strumarium (Asteraceae) was carried... more Antifungal activities and phytochemical screening of Xanthium strumarium (Asteraceae) was carried out in laboratory. Distilled water and methanol extracts of the leaves of plant was prepared. Five phytopathogenic fungi: Alternaria brassicae, Botrytis cinerea, Fusarium oxysporum, Phytophthora capsici and Sclerotium rolfsii were tested at different concentrations (50 mg/ml, 100 mg/ml, 150 mg/ml, 200 mg/ml, 250 mg/ml) of selected plant extracts. The phytochemical screening depicted the presence of terpenoids, saponins, flavonoids, tannins and alkaloids. The antifungal activity of extracts was determined by poisoned food technique; and linear mycelium growth reduction (LMGR) percentage was calculated. The distilled water extracts inhibited the growth of fungal mycelium while methanolic extracts completely inhibited (100%) the growth of some selected fungi at higher concentrations. Fusarium oxysporum was the most susceptible fungus while A. brassicae and B. cinerea were the most resistan...

Biological monitoring and assessment studies due to urban-road pollutants were carried out using ... more Biological monitoring and assessment studies due to urban-road pollutants were carried out using Air Pollution Tolerance Index (APTI) of plants. Four plant (leaf) parameters--namely ascorbic acid, total chlorophyll, relative water content and leaf extract pH were combined together in a formulation signifying the APTI of plants. APTI was calculated for five different shrub species viz. Bougainvillea glabra, Duranta repens, Lantana camara, Ricinus communis and Sambucus hookeri growing in three different areas, i.e. control area and along the roadsides in Kathmandu valley. The control site was selected in the Tribhuvan University, Campus area. Reduction in total chlorophyll content and pH was found in the leaf samples of all selected plants collected from less polluted sites (control) when compared with samples from more polluted site, whereas APTI, ascorbic acid and RWC were found to be higher in the plant samples of more polluted site as compared to less polluted site (control). From...

Antibacterial activities of Xanthium strumarium L. (Asteraceae) was carried out in laboratory. Di... more Antibacterial activities of Xanthium strumarium L. (Asteraceae) was carried out in laboratory. Distilled water and methanol extracts of the leaves of plant was prepared. The antibacterial activity was studied against six pathogenic bacteria, three gram negative: Klebsiella pneumoniae (ATCC 15380), Proteus mirabilis (ATCC 49132), Escherichia coli (ATCC 25922) and three gram positive: Bacillus subtilis (ATCC 6633), Enterococcus faecalis (ATCC 29212), Staphylococcus aureus (ATCC 25932) at different concentrations (50 mg/ml, 100 mg/ml, 150 mg/ml, 200 mg/ ml, 250 mg/ml) of leaf extracts of X. strumurium. The phytochemical screening depicted the presence of terpenoids, saponins, flavonoids, tannins and alkaloids. The antibacterial activity of extracts was determined by disc diffusion method and zone of inhibition (ZOI) was measured. Gram negative bacteria was found more resistant than gram positive bacteria. The most susceptible bacterium was S. aureus while the most resistant bacterium w...

International Journal of Pharmaceutical & Biological Archive, 2013

Chemical Composition of Essential Oils of Centella asiatica L Urban from Different Habitats of Nepal

The aqueous (distilled water) and alcoholic (methanol) crude extract from the leaves of invasive ... more The aqueous (distilled water) and alcoholic (methanol) crude extract from the leaves of invasive alien plant species Ageratina adenophora and Ipomoea carnea ssp. fistulosa were evaluated against six human pathogenic bacteria, three Gram negative: Escherichia coli (ATCC 25922) , Klebsiella pneumoniaea (ATCC 15380) and Proteus mirabilis (ATCC 49132) and three Gram positive: Enterococcus faecalis (ATCC 29212) Bacillus subtilis (ATCC 6633) and Staphylococcus aureus (ATCC 25923) using disc diffusion method. Different concentrations of plant extracts (50 mg/ml, 100 mg/ml, 150 mg/ml, 200 mg/ml and 250 mg/ml) were applied and diameter of zone of inhibition (ZOI) of bacterial growth were recorded. Both plant extracts showed antibacterial activity against Gram positive and Gram-negative bacteria. Methanolic extract of plant extracts exhibited good activity against tested bacteria when compared to aqueous extract. Among tested plants I. carnea ssp . fistula was more active than A. adenophora ....

Four community-managed Sal forests in Dadeldhura district of Nepal were selected for the assessme... more Four community-managed Sal forests in Dadeldhura district of Nepal were selected for the assessment of carbon stock. The stratified random sampling method was applied to measure the field data from 105 sample plots of 100 m2. The carbon stock of trees and shrubs was estimated using Chave allometric equations for moist forests. The study found that the community forests stored carbon stock ranging from 148.5 to 202.3 Mg ha–1 (mean: 175.5 Mg ha–1). The community forests managed for a longer duration have higher carbon stock than the community forests managed for a shorter duration. From the study, it can be concluded that these forests have been acting as a storehouse of the carbon. So, we can infer that community management has had a positive impact on increasing the carbon stock of forests and thus on the mitigation of global climate change. This study contributes to the understanding of the role of community forests in mitigating the effects of global climate change.

Wetlands are areas where water is the primary factor controlling the environment, associated plan... more Wetlands are areas where water is the primary factor controlling the environment, associated plant and animal life. The present study has been conducted to investigate the environmental variables of water and to make a quantitative analysis of aquatic macrophytes. Five lakes, i.e. Tamor, Beeshazar, Kingfisher, Athaieshazar and Chepang of the Chitwan National Park were studied during the post-monsoon season. Four different sites were studied for each lake. 12 replica of water samples were collected from each site of the lakes and environmental variables of water i.e. pH, temperature, dissolved oxygen, total alkalinity, conductivity, total hardness, nitrate, inorganic phosphorus, total solid matter and free CO2 were analyzed. Macrophyte floras were collected during field sampling with the help of a 1m×1m light wooden quadrat and herbaria were prepared following the standard technique. Altogether 10 quadrats (five paired quadrats) were plotted in each site of the study area. Data obtai...

The effect of variation in soil composition on growth vigour of Centella asiatica (L) Urb. was in... more The effect of variation in soil composition on growth vigour of Centella asiatica (L) Urb. was investigated in greenhouse of Central Department of Botany, Tribhuvan University,Kathmandu. Variation in different growth traits of Centella asiatica was investigated using vegetative clone of genome from one population in Kirtipur, Kathmandu, Nepal. We chose soil composition type as the treatment factor to study variation in growth traits as well as to know the best type of composition of soil for cultivation purpose. We raised plants in each of six soil compositional type and examined an array of vegetative traits like: number of leaves, petiole length, specific leaf area, number of primary branches and plant biomass. Most of the observed growth traits demonstrated significant variation in response to soil type. The C. asiatica plant can maximize growth and yield in habitat with sandy loam rather than clayey soil.