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6-OHDA Lesions and Behavioral Effects

6-OHDA, a neurotoxin, selectively destroys dopaminergic and noradrenergic neurons of the nigrostr... more 6-OHDA, a neurotoxin, selectively destroys dopaminergic and noradrenergic neurons of the nigrostriatal pathway. Similarly, in this research study, we did an MFB injection of 6-OHDA. Predicted rotational behavior is noted .

Figure 1. Nigrostriatal Pathway extends from substan- tia nigra (SN) to striatum (S) via medial forebrain bundle (MFB). Introduction to Neuroscience (226-01) | Prof. Turgeor Mohamed Ahmed Ramy ‘18 | 5/6/2016

Figure 3. Amphetamine-induced Rotational Behavior in Male prague- Dawley rats. Therefore, this test also served as an assessment to the extent of a lesion’s success.

Figure 4. Correlation between Ampheta- mine-induced and Apomorphine-induced Contrala- teral Rotations. figure serves to establish the different types of behavior because There is an inverse relationship, as expected. Com- parisons include R- 3, 4, 6, 8, 9, 10. From this small sample, we still recorded how apomorphine induces increased contralateral turning because it acts on the super sensitive dopamine receptors, in- creasing that side’s DA activity.

Scientists use rotational behavior to assess both lesion size and side- biased motor impairments.

Figure 6. Extent of Lesion and A pomorphine-induced rota- ‘ional behavior. Figure 5. Extent of Lesion and Amphetamine-induced rota- ional behavior. expected direction. This is observed in both drugs administered (Figure 5 and 6). It is conspicuous that the better the lesion, the more the rat tums in the expected direction. R9 had a 56.63% successful lesion, whereas R3 had a 99.70% one. The latter results in more ipsilat- eral rotations. Numbers represent the number of turns in the spe- cific direction as indicated by color. It is conspicuous that the better the lesion, the more the rat turns in the expected direction. R10 had a 1.13% successful lesion, whereas R8 had a 38% one. The former did not show the ex- pected behavior. Numbers represent the number of tums in the specific direction as indicated by color. below, it is apparent that lesions that are more accurate result in rats turning more often in the

Figure 7. Percent change in + c-Fos cell count in Dorsomedial Striatum for Five Rats. underwent c-Fos immunohistochemistry, we calculated the average percent change in c-Fos: It seems that right lesioned rats had significantly higher change in c-Fos count. This represents that neural activity was higher in the right-side of the brain of rats 2, 4, and 7. There was little to no difference between neural activity in the left and right side of the brains of rats 5 and 6.

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