Ulrich Schenck | Technical University of Munich (original) (raw)

Papers by Ulrich Schenck

Cancer, Dec 10, 2002

This review assessed the accuracy of rapid pre-screening (RPS) of routine cervical smears. The au... more This review assessed the accuracy of rapid pre-screening (RPS) of routine cervical smears. The authors concluded that RPS has the potential to be used as a quality control procedure in the screening process. The pooled measures reported should be viewed with caution, but the authors' recommendation for further research is justified. Authors' objectives To assess the accuracy of rapid pre-screening (RPS) of unreported cervical smears relative to subsequent full screening. Searching MEDLINE was searched; the search terms were reported. Other sources of primary studies were reference lists of retrieved papers and tables of contents of several specialised cytologic journals (to the end of 2001), which were handsearched. How were decisions on the relevance of primary studies made? The authors did not state how the papers were selected for the review, or how many reviewers performed the selection.

Cytopathology, 2006

Fine-needle aspiration cytology has become an accepted technique for the preoperative diagnosis o... more Fine-needle aspiration cytology has become an accepted technique for the preoperative diagnosis of palpable and radiographically detected lesions at a wide variety of body sites. Little information exists regarding the degree of utilization of the technique in the general medical community. The present study investigated the number of fine-needle aspirations (FNAs) performed in relationship to hospital size, surgical pathology caseload, and type of hospital. Six hospitals from each state were selected at random (total of 300), and a survey was sent which requested the following information: hospital size, surgical pathology caseload, FNA volume, most common sites aspirated, change in FNA utilization over the last 5 years, and who was performing the needle aspirates. Usable responses were received from 133 institutions. The survey revealed that in 63% of institutions, FNAs were performed predominantly or exclusively by clinicians. An increase in number of FNAs performed was reported in 73% of institutions, while 11% reported a net decrease in FNA volume. The breast was the most common organ undergoing needle aspiration, followed by the thyroid and lung. For all hospitals, irrespective of size and type, FNA volume represented about 2.5% of the total surgical pathology volume. For nonacademic tertiary-care institutions, FNA represented approximately 1.2% of the surgical pathology caseload, and for academic tertiary-care institutions, FNA volume was approximately 3.6% of cases. The survey indicated that utilization of FNA continues to increase, is predominantly performed by clinicians, and represents a greater percentage of tissue examinations in academic tertiarycare institutions than in either community general hospitals or nonacademic tertiary-care institutions.

Urologia Internationalis, 2000

Image Analysis & Stereology, Dec 1, 1985

Geburtshilfe und Frauenheilkunde, 1996

Gastrointestinal Endoscopy, 2004

Background: The accuracy of ERCP-based brush cytology or forceps biopsy for tissue diagnosis is r... more Background: The accuracy of ERCP-based brush cytology or forceps biopsy for tissue diagnosis is relatively low (usually not exceeding 70%). By contrast, reported accuracy rates for EUS-guided FNA of pancreatobiliary masses are over 80%. This prospective study compared these two modalities for the first time in the diagnosis of indeterminate biliary strictures and pancreatic tumors. Methods: Fifty consecutive patients (29 men, 21 women; mean age 62.1 years) with obstructive jaundice in whom a tissue diagnosis was required were included. During ERCP, intraductal specimens were obtained with a forceps and with two different types of brush (conventional and spiral suction) in random order. During EUS, only visible mass lesions or localized bile duct wall thickening were aspirated (22-gauge needle), with at least two passes yielding material sufficient for assessment. A cytopathologist was not present in the procedure room to evaluate specimen adequacy. The reference methods were surgery, other biopsy results, follow-up until death, or the conclusion of the study (mean follow-up 20 months). Results: The final diagnoses were malignancy, 28 (16 pancreatic, 12 biliary), and benign biliary stricture, 22. Sensitivity and specificity for ERCP-guided biopsy were 36% and 100%, respectively; for ERCP-guided cytology (when using conventional and spiral suction brushes), 46% and 100%, respectively; and for EUS-guided FNA, 43% and 100%, respectively. If the punctured lesions are considered (n = 28) alone, the sensitivity of EUS-guided FNA was 75%. In general, sensitivity was better for ERCP-based techniques in the subgroup biliary tumor (ERCP 75% vs. EUS 25%), whereas EUS-guided biopsy was superior for pancreatic mass (EUS 60% vs. ERCP 38%). Conclusions: For biliary strictures, combined ERCP-and EUS-guided tissue acquisition seems to be the best approach to tissue diagnosis. From a clinical standpoint, it appears reasonable, when a tissue diagnosis is required, to start with ERCP if biliary malignancy is suspected and with EUS when a pancreatic tumor is thought to be the cause of a biliary stricture. (Gastrointest Endosc 2004;60:390-6.

Experimental Cell Research, 1995

Diagnostic Cytopathology, 2003

Cytopathology has a long local and regional history in Germany, data in some areas going back to ... more Cytopathology has a long local and regional history in Germany, data in some areas going back to the time before surgical biopsy was practiced. Thanks to the pioneering efforts of Stoll, Zinser, Soost, Sprenger, Sandritter, Lennert, Droese, and others, cytopathology has become an integral

Acta Histochemica, 2008

The argyrophylic staining of the nucleolar organizer regions (AgNOR positive response) in interph... more The argyrophylic staining of the nucleolar organizer regions (AgNOR positive response) in interphase nuclei is often related directly to the cellular demand for ribosome biogenesis and is considered of relevance in studies of tumor pathology. Transformation of human breast epithelial MCF-10A cells by the c-Ha-ras oncogene results in altered growth, invasiveness and tumorigenicity in nude mice. Since ras transformation may be associated with a more intense nucleolar activity, we examined the influence of transfection by the Ha-ras oncogene on AgNOR staining response in MCF-10A cells. Following assessment of the AgNOR response with video image analysis, the AgNOR-positive areas and the AgNOR area/nuclear area ratio, but not the number of AgNOR aggregates or dots per nucleus, were found to be much higher after ras transformation. A role of the Ha-ras transformation on the nucleolar activity of the MCF-10A is thus suggested as assessed by the AgNOR staining. Based on data of the literature, it is also hypothesized that a decreased wild-type p53 level, possibly promoted by the ras transformation, may be associated with the increased AgNOR response.

Acta Cytologica, 1996

To assess continuous recording of the microscope status for quality assurance. Using a special mi... more To assess continuous recording of the microscope status for quality assurance. Using a special microscope with coordinate registration and objective decoding, 8,653 cervical smears were read by five experienced cytotechnologists. Cytotechnologists could check their screening pattern on a TV monitor. Stage position and magnification were registered every 20 msec. The average screening time per case was 3.5 minutes followed by an approximately two-minute intercase interval. Daily workload profiles were generated to check compliance with workload regulations. Average screening time over a day and over a week was rather constant. Our experience demonstrates that continuous coordinate registration can be easily implemented as a method of quality assurance in routine cytology.

The Japanese Journal of Urology, 2000

Acta Cytologica, 2000

Andrea Abati Fadi W. Abdul-Karim Pedro P. de Agustín Måns Åkerman M. Loredana Gadda Alasio Patric... more Andrea Abati Fadi W. Abdul-Karim Pedro P. de Agustín Måns Åkerman M. Loredana Gadda Alasio Patricia Alonso de Ruiz Carmen Alvarez-Santin Ana-Cristina Anton Karen M. Atkison Silvana Audy-Jurkovic Manon Auger R. Marshall Austin Jan P. A. Baak Ulrik Baandrup Gunter F. Bahr Peter H. Bartels Sandra H. Bigner John W. Bishop Alfred Böcking Miklós Bodó Vladimir N. Bogatyrev Thomas A. Bonfiglio Mathilde E. Boon Gerhard Breitenecker Ursula Buerzele-Fricke Jorge Campos R. de C. Carol A. Carriere Patricia Chapman William N. Christopherson Edith Claros Mercado Luiz Martins Collaco Brian T. Collins William N. Crabtree Peter Dalquen Dilip K. Das Diane D. Davey Irma DeLeón-Rodriguez Pranab Dey Manfred Droese Antonio Ducrot-Schinini Jaroslava Duskova S. Hamilton Dutoit Lars Egevad Hormoz Ehya Yener S. Erozan Dorothea Ferlin Clarice Amaral Ferreira Norman W. Fitzgerald Brendan T. Fitzpatrick Alessandra M. Forni Hugo Galera-Davidson Paolo Gattuso Kim R. Geisinger Claude Gompel Ricardo González-Cámpora Zenon Gonzalez-Romero Roberta M. Goodell Philippe de Graeve Shirley E. Greening Heinz K. Grohs Prabodh K. Gupta Raj K. Gupta Steven I. Hajdu Xhevdet Harasani Ernst Held Manuel Hilgarth Anders Hjerpe J. Heinrich Holzner O. A. N. Husain Martha L. Hutchinson Hideo Ikeda Lucrecia Illescas Stanley L. Inhorn Gita Jayaram Matías Jiménez-Ayala William W. Johnston David B. Kaminsky Kusum Kapila Harubumi Kato Ruth L. Katz William H. Kern Tadao K. Kobayashi Reviewers and Consultants

European Journal of Cancer, Nov 30, 2000

Cervical cancer is one of the target cancers covered by the statutory German cancer screening pro... more Cervical cancer is one of the target cancers covered by the statutory German cancer screening programme which was introduced in West Germany in 1971 and expanded to the eastern part of the country in 1991. Women covered by statutory health insurance (over 90% of the female population) are eligible to receive an annual cervical examination including a Papanicolaou (PAP) smear beginning at age 20 years. Annual uptake currently slightly exceeds 50% of the eligible population. Shortly after implementation of the national screening programme in the early 1970s the incidence of invasive cervical cancer decreased moderately and the incidence of cervical carcinoma in situ increased substantially in the state of Saarland. These observations would be expected as a result of a cervical cancer screening programme with substantial uptake. Although quality assurance guidelines for cervical cancer screening have been adopted and updated since the inception of the screening programme, only minor changes have been made in the cross-sectional programme documentation. Implementation of population-based documentation and evaluation of screening activities is currently being developed for the German cancer screening programme in pilot studies implementing the European guidelines on the quality assurance of mammography screening. After demonstration of feasibility and eectiveness, improvements in the quality management of breast cancer screening will subsequently be applied to the cervical cancer screening programme.

Gastrointestinal Endoscopy, 2009

EUS-guided FNA (EUS-FNA) is an established tissue-acquisition technique, with most studies concen... more EUS-guided FNA (EUS-FNA) is an established tissue-acquisition technique, with most studies concentrating on cytologic analyses of specimens, with only few data existing on histologic assessment. To assess the sensitivity of a combined analysis of histologic followed by cytologic tissue diagnosis. A retrospective 3-center study. In consecutive patients undergoing FNA of solid pancreatic masses, core specimens were harvested for histology; residual tissue was examined cytologically. Only unequivocally positive results were regarded as malignant. Criterion standards were positive results from EUS-FNA or other histologic findings, or, if negative, clinical follow-up data (minimum 12 months). Among 192 patients (110 men; mean age 63 years) with mostly pancreatic-head masses (72.4%), overall, adequate tissue was obtained in 98.9% of all cases, with a mean of 1.88 needle passes and an overall sensitivity of 82.9% (95% CI, 76.0%-88.5%). Histology and subsequent cytology provided adequate tissue and sensitivities of 86.5% and 60%, and 92.7% and 68.1%, respectively. Excluding cases with inadequate specimens, sensitivities rose by 4% to 10%. Histology showed a trend for superiority over cytology only in characterizing nonadenocarcinoma tumor types. No differences in sensitivity were found between the centers involved. Retrospective design, different processing of cytologic specimens. At EUS-FNA in pancreatic masses, combined histologic-cytologic analysis achieved a sensitivity of more than 80%, despite a low number of needle passes and may thus save time. Histology alone did not reach higher sensitivity than cytology. In particular situations, eg, rare tumors, histology may still be required.

Gastrointestinal Endoscopy, 2006

Cancer, 1998

It has been established that comparative genomic hybridization (CGH) on Papanicolaou-stained cerv... more It has been established that comparative genomic hybridization (CGH) on Papanicolaou-stained cervical smears can be used to identify chromosomal imbalances. In this study, the authors identified normal and dysplastic squamous epithelial cells cytologically, eliminated surrounding bacteria or leukocytes by a ultraviolet laser microbeam under microscopic control, and scraped out the cell groups of interest by a microdissection system. In 3 cases of squamous intraepithelial lesions (SIL), a total of 9 samples of dysplastic (n = 6) and nontumorous cells (n = 3) were investigated, each of them consisting of 3-40 cells. The DNA was amplified by degenerate oligonucleotide primed PCR (DOP-PCR) and used for CGH. Analyses of all nontumorous cell groups resulted in fluorescence ratio profiles that showed no deviation from the normal range, confirming that no methodologic artefacts have been produced. The CGH profiles from dysplastic cells, however, showed various chromosomal imbalances affecting six to nine different chromosomes. The most frequent gains in DNA were observed on chromosomes 1p, 2q, 4, and 5, whereas losses were found on chromosomes 6q and 13q. The results of this study demonstrate the feasibility and reliability of CGH on microdissected cell samples of routinely processed cervical smears. To the authors' knowledge, this is the first study reporting the use of CGH on cervical routine smears. This approach offers the opportunity to investigate sequence copy number changes in small, morphologically well-defined groups of dysplastic cells. It may, therefore, serve as a cytogenetic screening test for identifying chromosomal aberrations in precancerous lesions that are associated with a high risk for progression to invasive cancer.

Andrologia, 2009

Vergleichende U ntersuchu ng zw ischen Hodenbiopsie und Biopsieausstrich bei Azoospermie" U. SCHE... more Vergleichende U ntersuchu ng zw ischen Hodenbiopsie und Biopsieausstrich bei Azoospermie" U. SCHENCK und W.-B. SCHILL Einleitung lung des Ejakulatausstrichs ein wenig beachtetes Gebiet der andrologischurologischen Diagnostik. Hodenpunktionen zur andrologischen Diagnostik wurden schon um die Jahrhundertwende von Posner und Cohn (19O4), Posner (1905) sowie von Huhner (1913, 1928) durchgefuhrt, wobei in der Regel in ungefarbten Praparaten nach Spermatozoen gesucht wurde. Fanden sich Spermatozoen, so galt dies als Zeichen fiir eine Verschlufiazoospermie (Posner u. Cohn-1904). (Charny-1940), die sich im Laufe der 50er und 60er Jahre als bilaterale operative Biopsie zur Methode der Wahl in der andrologischen Hodenmorphologie entwickelte (Jordan-1966). Aber bereits 1934 hatten Schockaert und Ferin (1953) uber Hoden-*Auszugsweise vorgetragen bei der 8. Dreiliindertagung fd Klinische Zytologie, Freiburg i.Br.,