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Papers by H. Festl
International Journal of Systematic Bacteriology, 1985
Ribosomal ribonucleic acid (rRNA) genes were isolated from a PstI digest of Pseudomonas aeruginos... more Ribosomal ribonucleic acid (rRNA) genes were isolated from a PstI digest of Pseudomonas aeruginosa chromosomal deoxyribonucleic acid (DNA), cloned in Escherichia culi, and used as probes for conserved gene sequences. Recombinant plasmid pHFl contained an 8,800-base pair insertion containing 5S, 16S, and 23s rRNA genes. We constructed subclones of pHFl containing parts of the 16s and 23s rRNA genes (pHFl.l) and parts of the 23s and 5s rRNA genes (pHF1.2). DNA-DNA hybridization experiments in which we used filter-bound chromosomal DNA from various bacteria and 35S-labeled plasmid rRNA genes (rDNA) indicated that the homology values reflected the actual phylogenetic distances to P. aeruginosa. Compared with oligonucleotide sequence analysis of 16s rRNA, a good correlation was found between DNA-rDNA homology values and SAB (similarity coefficient of 16s rRNAs) values above 0.4. The use of rDNA instead of rRNA in hybridization experiments offers several advantages; e.g., rDNA can easily be labeled in vitro, and the degree of relatedness can be expressed in terms of percent homolgy and does not have to be determined by laborious measurement of thermal stability, as in the case of rRNA.
Applied and environmental microbiology, 1986
Plasmid pHF360 was constructed from cloned rRNA genes (rDNA) of Pseudomonas aeruginosa and used a... more Plasmid pHF360 was constructed from cloned rRNA genes (rDNA) of Pseudomonas aeruginosa and used as hybridization probe for the Pseudomonas fluorescens group. The probe was tested by dot and in situ colony hybridizations to chromosomal DNAs from a wide variety of organisms. pHF360 DNA hybridized exclusively to chromosomal DNAs from bacteria representing the P. fluorescens group and separated them clearly from all other bacteria tested in the present study. Determination of the nucleotide sequence of the cloned DNA showed that it is a fragment from a 23S rRNA gene of P. aeruginosa. It was compared with the published 23S RNA sequence from Escherichia coli.
Systematic and applied microbiology, 1983
The optical method of De Ley et al. (1970) for determining DNA/DNA homologies was reexamined. Sev... more The optical method of De Ley et al. (1970) for determining DNA/DNA homologies was reexamined. Several parameters such as incubation temperature, incubation time, DNA concentration and DNA fragment length were checked and the optimal conditions established. Hybridization data of several anaerobic Gram-positive cocci were compared with values obtained by the membrane filter technique. The agreement is excellent above a degree of binding of 25-30%.
Deoxyribonucleic acid-deoxyribonucleic acid reassociation and deoxyribonucleic acid-ribosomal rib... more Deoxyribonucleic acid-deoxyribonucleic acid reassociation and deoxyribonucleic acid-ribosomal ribonucleic acid cistron similarity studies showed that the anaerobic, gram-positive cocci comprise a rather heterogeneous group of bacteria. The deoxyribonucleic acid-ribosomal ribonucleic acid hybridization studies distinguished seven groups. Groups 1 and 2 consisted of Peptostreptococcus magnus and Peptostreptococcus prevotii, respectively. Peptostreptococcus asaccharolyticus ATCC 14963T (T = type strain) and Peptostreptococcus indolicus ATCC 29427T formed a third group, and Peptostreptococcus asaccharolyticus DSM 20364 together with Hare group VIII strain NCTC 9820 formed group 4. Peptostreptococcus anaerobius DSM 20357 was more closely related to Eubacterium tenue ATCC 25553T and Clostridium lituseburense ATCC 25759T than to any of the other species studied. Peptostreptococcus micros strains DSM 2046gT and DSM 20367 together with strains belonging to Hare group IX formed group 6, and group 7 consisted of Peptostreptococcus pawulus DSM 20469T. Strains of different Hare groups were all assigned to one of the seven groups or to the genus Staphylococcus (Hare group VIIb strain NCTC 9819) or the genus Streptococcus (Hare group VIa strain NCTC 9806). The anaerobic cocci also have a diversity of murein structures. This is the first report showing that strains belonging to the same species have different murein types.
International Journal of Systematic Bacteriology, 1985
Ribosomal ribonucleic acid (rRNA) genes were isolated from a PstI digest of Pseudomonas aeruginos... more Ribosomal ribonucleic acid (rRNA) genes were isolated from a PstI digest of Pseudomonas aeruginosa chromosomal deoxyribonucleic acid (DNA), cloned in Escherichia culi, and used as probes for conserved gene sequences. Recombinant plasmid pHFl contained an 8,800-base pair insertion containing 5S, 16S, and 23s rRNA genes. We constructed subclones of pHFl containing parts of the 16s and 23s rRNA genes (pHFl.l) and parts of the 23s and 5s rRNA genes (pHF1.2). DNA-DNA hybridization experiments in which we used filter-bound chromosomal DNA from various bacteria and 35S-labeled plasmid rRNA genes (rDNA) indicated that the homology values reflected the actual phylogenetic distances to P. aeruginosa. Compared with oligonucleotide sequence analysis of 16s rRNA, a good correlation was found between DNA-rDNA homology values and SAB (similarity coefficient of 16s rRNAs) values above 0.4. The use of rDNA instead of rRNA in hybridization experiments offers several advantages; e.g., rDNA can easily be labeled in vitro, and the degree of relatedness can be expressed in terms of percent homolgy and does not have to be determined by laborious measurement of thermal stability, as in the case of rRNA.
Applied and environmental microbiology, 1986
Plasmid pHF360 was constructed from cloned rRNA genes (rDNA) of Pseudomonas aeruginosa and used a... more Plasmid pHF360 was constructed from cloned rRNA genes (rDNA) of Pseudomonas aeruginosa and used as hybridization probe for the Pseudomonas fluorescens group. The probe was tested by dot and in situ colony hybridizations to chromosomal DNAs from a wide variety of organisms. pHF360 DNA hybridized exclusively to chromosomal DNAs from bacteria representing the P. fluorescens group and separated them clearly from all other bacteria tested in the present study. Determination of the nucleotide sequence of the cloned DNA showed that it is a fragment from a 23S rRNA gene of P. aeruginosa. It was compared with the published 23S RNA sequence from Escherichia coli.
Systematic and applied microbiology, 1983
The optical method of De Ley et al. (1970) for determining DNA/DNA homologies was reexamined. Sev... more The optical method of De Ley et al. (1970) for determining DNA/DNA homologies was reexamined. Several parameters such as incubation temperature, incubation time, DNA concentration and DNA fragment length were checked and the optimal conditions established. Hybridization data of several anaerobic Gram-positive cocci were compared with values obtained by the membrane filter technique. The agreement is excellent above a degree of binding of 25-30%.
Deoxyribonucleic acid-deoxyribonucleic acid reassociation and deoxyribonucleic acid-ribosomal rib... more Deoxyribonucleic acid-deoxyribonucleic acid reassociation and deoxyribonucleic acid-ribosomal ribonucleic acid cistron similarity studies showed that the anaerobic, gram-positive cocci comprise a rather heterogeneous group of bacteria. The deoxyribonucleic acid-ribosomal ribonucleic acid hybridization studies distinguished seven groups. Groups 1 and 2 consisted of Peptostreptococcus magnus and Peptostreptococcus prevotii, respectively. Peptostreptococcus asaccharolyticus ATCC 14963T (T = type strain) and Peptostreptococcus indolicus ATCC 29427T formed a third group, and Peptostreptococcus asaccharolyticus DSM 20364 together with Hare group VIII strain NCTC 9820 formed group 4. Peptostreptococcus anaerobius DSM 20357 was more closely related to Eubacterium tenue ATCC 25553T and Clostridium lituseburense ATCC 25759T than to any of the other species studied. Peptostreptococcus micros strains DSM 2046gT and DSM 20367 together with strains belonging to Hare group IX formed group 6, and group 7 consisted of Peptostreptococcus pawulus DSM 20469T. Strains of different Hare groups were all assigned to one of the seven groups or to the genus Staphylococcus (Hare group VIIb strain NCTC 9819) or the genus Streptococcus (Hare group VIa strain NCTC 9806). The anaerobic cocci also have a diversity of murein structures. This is the first report showing that strains belonging to the same species have different murein types.