M. Rezayat | Tehran University of Medical Sciences (original) (raw)

Papers by M. Rezayat

Journal of Alloys and Compounds, 2016

Pharmacology & toxicology, 1999

In a previous work, the effects of cholecystokinin receptor agonists on tolerance to morphine ant... more In a previous work, the effects of cholecystokinin receptor agonists on tolerance to morphine antinociception were evaluated. In the present study, the influence of cholecystokinin antagonists on the inhibition of tolerance to morphine antinociception by cholecystokinin agonists has been investigated. Maximum tolerance to morphine antinociception was obtained by morphine administration (50 mg/kg) to mice once daily for 4 days. The cholecystokinin receptor agonists caerulein (0.005 mg/kg) or cholecystokinin-8 (0.01 mg/kg) but not unsulfated cholecystokinin-8 (0.01 mg/kg) decreased the development of tolerance to morphine (9 mg/kg). The cholecystokininA receptor antagonist MK-329 (1 mg/kg) or the cholecystokininB receptor antagonist L-365,260 (0.25, 0.5 and 1 mg/kg) also diminished the tolerance to morphine antinociception. When animals were challenged with different doses of MK-329 (0.25, 0.5 and 1 mg/kg) against cholecystokinin-8 (0.01 mg/kg), caerulein (0.005 mg/kg) or unsulfated c...

General Pharmacology: The Vascular System, 1994

l. In the present study, the effect of caerulein (CLN) on morphine analgesia has been tested. 2. ... more l. In the present study, the effect of caerulein (CLN) on morphine analgesia has been tested. 2. Different doses of morphine produced antinociception in a dose dependent manner. When animals were pretreated with CLN 5 min before morphine administration, the morphine response was decreased. However, when CLN was injected 30 or 60 min prior to morphine, the drug effect was increased.

European Neuropsychopharmacology, 1999

In the present study, the dopaminergic receptor agonist apomorphine (0.1, 0.25 and 0.5 mg / kg) i... more In the present study, the dopaminergic receptor agonist apomorphine (0.1, 0.25 and 0.5 mg / kg) induced a dose-dependent licking in rats. Nicotine administration (0.025-250 mg / kg) altered the apomorphine-induced licking. The lower doses of nicotine (0.05 and 0.5 mg / kg) increased while the higher dose of the drug (250 mg / kg) reduced the apomorphine response. The antimuscarinic drug atropine (2.5 and 5 mg / kg) reduced the effects of apomorphine or nicotine plus apomorphine. The central nicotinic receptor antagonist mecamylamine (0.05, 0.25 and 0.5 mg / kg) also reduced the response induced by apomorphine or nicotine plus apomorphine. However, the peripheral nicotinic receptor antagonist hexamethonium (2.5, 5 and 10 mg / kg) reduced the response induced by nicotine plus apomorphine but not that elicited by apomorphine alone. The results indicate that the nicotinic receptor mechanism(s) may interact with apomorphine-induced licking in rats. Although central nicotinic and cholinergic mechanisms may be involved in the licking induced by apomorphine, peripheral nicotinic mechanism may be involved in the nicotine-induced increased apomorphine effect.

European Journal of Pharmacology, 1994

Different groups of mice were treated with morphine (50 mg/kg s.c.) once daily for 1, 2, 3, 4 or ... more Different groups of mice were treated with morphine (50 mg/kg s.c.) once daily for 1, 2, 3, 4 or 5 days, in order to develop tolerance to the drug. The antinociceptive effect of morphine (9 mg/kg s.c.) was tested 24 h after each dose of the drug administration. Tolerance to morphine reached its peak on the 4th day. Daily pretreatment of animals for a period of 4 days with different doses of cholecystokinin octapeptide (CCK-8; 0.001, 0.01, 0.05 and 0.1 mg/kg s.c.), caerulein (0.0001, 0.001, 0.005 and 0.01 mg/kg s.c.) but not unsulfated cholecystokinin octapeptide (0.001, 0.01 or 0.1 mg/kg s.c.) 30 min before daily administration of morphine (50 mg/kg s.c.) prevented the development of tolerance. A group of animals received a single dose of caerulein (0.005 mg/kg), CCK-8 (0.01 mg/kg) or unsulfated CCK-8 (0.01 mg/kg) 30 min before morphine injection (50 mg/kg s.c.) on the 3rd or 4th day. In these animals, which were tested for antinociception on the 5th day, tolerance to the drug (3, 6 and 9 mg/kg s.c.) was also decreased by caerulein, CCK-8 but not unsulfated CCK-8. In a group of mice in which peptides were administered 30 min prior to the doses of morphine (3, 6 or 9 mg/kg s.c.) on the 5th day, similar results were obtained. The results of the present study indicate that activation of both CCK B and CCK A receptors may prevent the development of tolerance to morphine, and the sulfate group in the CCK-8 molecule may be essential for the tolerance inhibition.

European Journal of Pharmacology, 1995

The effect of 5-HT receptor antagonists on tolerance to morphine antinociception was studied in m... more The effect of 5-HT receptor antagonists on tolerance to morphine antinociception was studied in mice. Slow release morphine suspension was injected subcutaneously (s.c.) in order to produce tolerance. When different doses of morphine (3, 6 and 9 mg/kg) were administered on the 4th day after injection of slow-release morphine suspension, tolerance to the test doses of morphine was observed. The tolerance obtained was decreased by pretreatment with the non-selective 5-HT receptor antagonist methysergide (1 and 2 mg/kg) or the 5-HT 2 receptor antagonist ritanserin (1 and 2 mg/kg). When the 5-HT receptor antagonists were used on the 2nd and 3rd day after injection of slow-release morphine suspension or on the 4th day (60 min before last dose of morphine), a maximum reduction in morphine tolerance was observed on the 3rd day. Pretreatment of animals with metergoline (l and 2 mg/kg) or mianserin (1 and 2 mg/kg) also decreased the tolerance to morphine. It may be concluded that at least a 5-HT 2 receptor mechanism is involved in tolerance to morphine antinociception.

Acta Medica Iranica, 2004

Page 1. EFFECT OF APAMIN ON TOLERANCE TO COCAINE-INDUCED LOCOMOTOR ACTIVITY IN MICE HR Jamshidi, ... more Page 1. EFFECT OF APAMIN ON TOLERANCE TO COCAINE-INDUCED LOCOMOTOR ACTIVITY IN MICE HR Jamshidi, M. Rezayat* and MR Zarrindast Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran ...

Basic & Clinical Pharmacology & Toxicology, 2011

Diabetic neuropathy (DN) is the most common peripheral neuropathy and long-term complication of d... more Diabetic neuropathy (DN) is the most common peripheral neuropathy and long-term complication of diabetes. In view of the pathological basis for the treatment of DN, it is important to prevent nerve degeneration. Most of the current treatment strategies are symptomatic therapies. In this study, we evaluated the effectiveness of magnesium-25, carrying porphyrin-fullerene nanoparticles, on diabetes-induced neuropathy. Previous studies have suggested that dorsal root ganglion (DRG) neurons comprise a specific target and may be responsible for the known complications of DN. Experimental DN was induced by intraperitoneal injection of streptozotocin (STZ) (45 mg/kg). Different forms of magnesium including (25)Mg-PMC16, (24)Mg-PMC16 and MgCl(2) were administered intravenously in equal dose (0.5 LD(50)) at 48-hr intervals before STZ injection. Peripheral nerves were studied after 2 months of diabetes in groups using qualitative approaches, morphometric analysis of DRG neurons and motor function tests. We showed that STZ-induced DN caused morphological abnormalities in DRG neurons comprising changes in area, diameter and number of A and B cells as well as motor dysfunction in DN. Moreover, our findings indicated that administration of (25)Mg-PMC16 as a magnetic form of Mg improved morphological abnormalities and motor dysfunctions significantly, whereas other forms of Mg were ineffective.

Pharmacology & Toxicology, 1998

In this work, the influences of cholecystokinin receptor antagonists L-365,260, MK-329 and proglu... more In this work, the influences of cholecystokinin receptor antagonists L-365,260, MK-329 and proglumide on antinociception induced by baclofen and GABA uptake inhibitor 4,5,6,7-tetrahydroisoxazolo [4,5-c]pyridin-3-ol (THPO) in the tail flick test has been studied. Baclofen and THPO induced antinociception in the tail flick test. Morphine, and the CCK receptor antagonists, MK-329, L-365,260 and proglumide also induced antinociception. The CCK receptor antagonists potentiated antinociceptive response induced by both baclofen and THPO. It may be concluded that cholecystokinin receptor mechanism(s) may interact with antinociception induced by GABA receptor mechanism(s).

Archives Of Physiology And Biochemistry, 1995

The role of cholecystokinin (CCK) in the development of a necrotizing acute pancreatitis induced ... more The role of cholecystokinin (CCK) in the development of a necrotizing acute pancreatitis induced by a diet deficient in choline and supplemented with ethionine (CDE) has been evaluated in the rat by using a potent CCK receptor antagonist L-364,718. Acute pancreatitis was induced by administration of CDE diet for 14 days. L-364,718 administration was carried out by subcutaneous injections at dose of 0.1 mg/kg/day. Pancreatic exocrine secretion (flow, protein, amylase and trypsin outputs) in resting and under infusion of 1.25 microgram/kg/h of CCK-8 were used to evaluate the pancreatic functionality. Others parameters (serum amylase, percentage fluid in pancreas, haematocrit and mortality) evaluated the severity of pancreatitis. L-364,718 slightly reduced the mortality and the increases of percentage of fluid accumulated in pancreas in CDE diet acute pancreatitis. Basal and CCK stimulated pancreatic secretion was significantly depressed 36 hours after L-364,718 treatment. A slight response to CCK was observed. Nevertheless it was lower than usually observed in control rats. Our results demonstrate that in the rat, chronic L-364,718 treatment did not completely restore pancreatic activity in acute pancreatitis induced by CDE diet. Hence CCK cannot be considered as the main factor involved in the development of this pancreatitis model.

A very important obstacle in axonal regeneration after spinal cord injury is astroglial scaring. ... more A very important obstacle in axonal regeneration after spinal cord injury is astroglial scaring. Noggin as bone morphogenic protein inhibitor plays a critical role in decreasing GFAP 1 cells and reducing the number of astrocytes in the site of injury. Human endometrial-derived stromal cells (hEnSCs) were isolated and cultured in two different neural inductive mediums consisting of neural progenitor maintenance medium (NPMM)/BDNF or NPMM/BDNF/Noggin in Matrigel 3D cell culture. Neural expression markers were investigated at the mRNA and protein level by real-time PCR and immunocytochemistry, respectively. The results showed that Noggin supplementation was able to increase the expression of Nestin, Tuj-1, and NF, whereas the expressions of GFAP, Bcl2, and Olig2 were decreased. In addition, DAPI staining demonstrated that lighter blue chromatin agreed with our observation of lower level of Bcl2 expression in the Noggin protocol in which over-expression of Bcl2 gene did not induce higher neurogenesis in poor Noggin medium. Our findings clearly demonstrated the neural differentiation potential of hEnSC in Matrigel and also Noggin supplementation was able to inhibit astrocyte formation. V C 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 00A:000-000, 2014. How to cite this article: Tavakol S, Mousavi SMM, Masummi M, Amani A, Rezayat SM, Ai J. 2014. The effect of Noggin supplementation in Matrigel nanofiber-based cell culture system for derivation of neural-like cells from human endometrialderived stromal cells. J Biomed Mater Res Part A 2014:00A:000-000.

Bone matrix consists of two major phases at the nanoscale: organic and hydroxyapatite. Nanotechno... more Bone matrix consists of two major phases at the nanoscale: organic and hydroxyapatite. Nanotechnology as a diverse and interdisciplinary area of research has the capacity to revolutionise many areas of applications such as bone tissue engineering. Nanohydroxyapatite/gelatin composite has higher osteoblast attachment and proliferation than micro-sized ones, and shorter culturing period and lower cell seeding density compared to pure gelatin. A nanostructured scaffold was fabricated by three methods for bone repair using nanohydroxyapatite and gelatin as the main components. Its biocompatibility, alizarin red test on the 14th and 21st days, gene expression on the 21st day in in vitro using and histomorphometry after 4 and 8 weeks postimplantation in the rat were investigated. Cultured unrestricted somatic stem cells used for in vitro study showed an excellent level of cell attachment to the scaffold. Cells induced more osteoblast differentiation on the scaffold than in 2D cell culture. Osteoblast differentiation and bone regeneration results of in vitro and in vivo investigation on scaffold were extremely significant, better than control and treatment groups. These effects could be attributed to the shape and size of nanoHA particles and good architecture of the scaffold. The results confirm the feasibility of bone regeneration using synthesised scaffold as a temporary bone substitute.

Medical Journal of the Islamic Republic of Iran (MJIRI), May 15, 2001

The environmental pollutant 2, 3, 7, 8-tetra chlorodibenzo-p-dioxin (TCDD) has received much at t... more The environmental pollutant 2, 3, 7, 8-tetra chlorodibenzo-p-dioxin (TCDD) has received much at tention over the past few decades.! It is a highly toxic compound which is found as a contaminant in phenoxy acid, herbicides, chlorophenol, and also as a by-product during synthesis of industrial halogenated compounds. It has also been produced by various combustion pro-

Med. J. Islam. Repub. Iran, May 1, 2001

International Journal of Pharmacology, 2005

ABSTRACT The purpose of this study was to evaluate protective effects of vitamin E, selenium and ... more ABSTRACT The purpose of this study was to evaluate protective effects of vitamin E, selenium and propranolol on TCDD induced changes of biochemical parameters using the rat liver perfusion system. Various concentrations of TCDD (0.3, 3, 20, 30 �g L-1) were added to the perfusion fluid and biochemical changes in perfusion fluid of isolated rat liver were examined within 2 h. The results showed that TCDD significantly increases the aminotransferase activities in a dose-dependent manner. It was determined that TCDD at 20 �g L-1 caused a maximum increase in biochemical parameters within 2 h (p<0.0001). The result also showed that propranolol (20 mg L-1), sodium selenite (345 mg L-1) and vitamin E (α-tocopherol, 700 mg L-1) significantly decreases TCDD hepatotoxicity. Aminotransferase enzyme �activity �as well as glutathione and protein content significantly changed in treatment groups as compared to the TCDD group (p<0.001). Reduction in hepatotoxicity may be attributed to prevention of lipid peroxidation, although other mechanisms may also be involved.

Archives of Iranian medicine, 2010

Serotonin (5HT) has been shown to be a mitogenic factor in several carcinomas. Its mitogenic effe... more Serotonin (5HT) has been shown to be a mitogenic factor in several carcinomas. Its mitogenic effect is elicited through a wide range of 5HT receptor subtypes. In this study, the effects of 5HT, 5HT3 (1-phenylbiguanide hydrochloride) and 5HT4 (cisapride) agonists in promoting the growth of the HT29 cell line and the growth-inhibition effect of the 5HT3 receptor antagonist (Y-25130 hydrochloride) and 5HT4 receptor antagonist (RS 23597-190) were investigated. The expressions of 5HT3 and 5HT4 receptors in human colon cancer tissues and the HT29 cell line were studied. The growth-promoting and growth-inhibition effects of 5-HT, 5HT3 and 5HT4 agonists and antagonists on the HT29 cell line were studied using MTT assay. Receptor expression has been demonstrated by western blotting. The results showed that 5HT, 5HT3, and 5HT4 agonists caused significant proliferation of HT29 cells. 5HT3 and 5HT4 receptor antagonists had an inhibitory effect on the growth of these cells. Western blot analysis...

Materials Science and Technology, 2012

Aluminium matrix composites reinforced with submicrometre and nanosize Al 2 O 3 particles were su... more Aluminium matrix composites reinforced with submicrometre and nanosize Al 2 O 3 particles were successfully manufactured in the form of sheets through eight cycles of accumulative roll bonding process. The mechanical properties of the produced composite are compared with accumulative roll bonded commercially pure aluminium. It is shown that only 1 vol.-% of submicrometre or nanosize alumina particles as reinforcement in the structure can significantly improve the yield and ultimate tensile strengths. Scanning electron microscopy revealed that particles have a random and uniform distribution in the matrix especially in the less volume fraction of alumina particles, and strong mechanical bonding occurs at the interface of the particle matrix. According to the results of the tensile tests, it is observed that with less alumina content, the composite reinforced by nanosize particles has higher strength than that by submicrometre size particles. However, more reinforcement up to 3 vol.-% of submicrometre particles, as a result of including fewer microstructural defects, leads to better mechanical properties in comparison to the nanoparticle composite.