Omid Sabzevari | Tehran University of Medical Sciences (original) (raw)
Phone: +98 21 6649 4994
Address: Department of Toxicology & Pharmacology
Faculty of Pharmacy
Tehran University of Medical Sciences (TUMS)
Tehran-14155/6451, IRAN
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Papers by Omid Sabzevari
Background: Sepsis is a debilitating systemic disease and described as a severe and irregular sys... more Background: Sepsis is a debilitating systemic disease and described as a severe and irregular systemic inflammatory reaction syndrome (SIRS) against infection. We employed CLP (Cecal Ligation and Puncture) model in rats to investigate anti-inflammatory and antioxidant effects of phloretin, as a natural antioxidant agent, and its protective effect on liver tissue damage caused by sepsis.
Biochemical Society Transactions, 2000
Drug research, Jan 25, 2014
CYP1A1, a P450 isoenzyme, is involved in the phase I xenobiotic metabolism including teratogen dr... more CYP1A1, a P450 isoenzyme, is involved in the phase I xenobiotic metabolism including teratogen drugs. In the present study, the ability of teratogens to elevate the embryonic expression of CYP1A1 was examined. Micromass cell cultures prepared from day 13 rat embryo limb buds (LB). LB cells were cultivated and exposed for 5 days to retinoic acid (RA), hydrocortisone (HC), caffeine (CA) and quinine (QN). CYP1A1 protein expression and activity were measured using immunofluorescence staining and ethoxyresorufin O-deethylation (EROD) assay, respectively. The EROD activity increased significantly following LB cells exposure to RA and HC (p<0.05) but the expression of CYP1A1 protein was reduced by these drugs, whereas the expression of CYP1A1 protein and EROD activity decreased significantly following the addition of CA and QN (p<0.05, p<0.01). Our findings show that studied teratogens have potency to increase CYP1A1 activity.
Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences, 2011
Thymoquinone (TQ) is one of the active components of Nigella sativa. The plant has been used in h... more Thymoquinone (TQ) is one of the active components of Nigella sativa. The plant has been used in herbal medicine for treatment of many diseases including liver complications. The present study aimed to investigate protective effects of TQ on Aflatoxin B(1) (AFB(1)) induced liver toxicity in mice. Animals were divided into six groups and treated intraperitoneally. Group 1 (blank) served as vehicle, group 2 (positive control) received AFB(1), Group 3 was treated with 9 mg/kg of TQ, Groups 4, 5 and 6 were treated with 4.5, 9 and 18 mg/kg of TQ, respectively. After three consecutive days, except for groups 1 and 3, animals were administered with a single dose of AFB(1) (2 mg/kg). All the animals were killed 24 hrs following the AFB(1) administration under ether anesthesia. Biochemical parameters including AST, ALT and ALP in serum samples and glutathione (GSH) and malondialdehyde (MDA) contents in liver homogenates were determined. Liver sections were collected for histopathological exam...
International Journal of Pharmacology, 2010
ABSTRACT In this study, Nasturtium officinale R. Br. (watercress), an edible plant from crucifero... more ABSTRACT In this study, Nasturtium officinale R. Br. (watercress), an edible plant from cruciferous plants and its three fractions were screened for their hepatoprotective effects against acetaminophen induced toxicity in rats. The total parts of plant were extracted with alcohol-water and then sequentially fractionated. The total extract and its three fractions namely; petroleum ether; n -butyl alcohol; and aqueous at two doses (50 and 175 mg kg-1) were administrated to animals every 24 h for five successive days. Rats treated as post treatment with the total extract or three fractions at the dose of 175 mg kg-1 did not show any significance change in activity of aminotransferse enzymes (AST and ALT) compared to control. Acetaminophen caused significant hepatocellular damage and increase in serum levels of AST, ALT and LDH in comparison to control group (p<0.001). Post treatment with the total extract (175 mg kg-1) and aqueous fraction (50 mg kg-1) significantly prevented acetaminophen induced rise in serum levels of AST, ALT and LDH. LD50 value of the petroleum fraction was more than 3823 mg kg-1, while the other two fractions and total extract were non-toxic up to 5734 mg kg-1. Histopathological changes of liver induced by acetaminophen were considerably reversed following treatment by aqueous fraction and total extract of N. officinale . The findings of this study showed that N. officinale may play a protective role against acetaminophen-induced hepatotoxicity through maintaining the normal liver functions which further validates the use of N. officinale in Iranian traditional medicine as a hepatoprotective agent.
Background: Sepsis is a debilitating systemic disease and described as a severe and irregular sys... more Background: Sepsis is a debilitating systemic disease and described as a severe and irregular systemic inflammatory reaction syndrome (SIRS) against infection. We employed CLP (Cecal Ligation and Puncture) model in rats to investigate anti-inflammatory and antioxidant effects of phloretin, as a natural antioxidant agent, and its protective effect on liver tissue damage caused by sepsis.
Biochemical Society Transactions, 2000
Drug research, Jan 25, 2014
CYP1A1, a P450 isoenzyme, is involved in the phase I xenobiotic metabolism including teratogen dr... more CYP1A1, a P450 isoenzyme, is involved in the phase I xenobiotic metabolism including teratogen drugs. In the present study, the ability of teratogens to elevate the embryonic expression of CYP1A1 was examined. Micromass cell cultures prepared from day 13 rat embryo limb buds (LB). LB cells were cultivated and exposed for 5 days to retinoic acid (RA), hydrocortisone (HC), caffeine (CA) and quinine (QN). CYP1A1 protein expression and activity were measured using immunofluorescence staining and ethoxyresorufin O-deethylation (EROD) assay, respectively. The EROD activity increased significantly following LB cells exposure to RA and HC (p<0.05) but the expression of CYP1A1 protein was reduced by these drugs, whereas the expression of CYP1A1 protein and EROD activity decreased significantly following the addition of CA and QN (p<0.05, p<0.01). Our findings show that studied teratogens have potency to increase CYP1A1 activity.
Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences, 2011
Thymoquinone (TQ) is one of the active components of Nigella sativa. The plant has been used in h... more Thymoquinone (TQ) is one of the active components of Nigella sativa. The plant has been used in herbal medicine for treatment of many diseases including liver complications. The present study aimed to investigate protective effects of TQ on Aflatoxin B(1) (AFB(1)) induced liver toxicity in mice. Animals were divided into six groups and treated intraperitoneally. Group 1 (blank) served as vehicle, group 2 (positive control) received AFB(1), Group 3 was treated with 9 mg/kg of TQ, Groups 4, 5 and 6 were treated with 4.5, 9 and 18 mg/kg of TQ, respectively. After three consecutive days, except for groups 1 and 3, animals were administered with a single dose of AFB(1) (2 mg/kg). All the animals were killed 24 hrs following the AFB(1) administration under ether anesthesia. Biochemical parameters including AST, ALT and ALP in serum samples and glutathione (GSH) and malondialdehyde (MDA) contents in liver homogenates were determined. Liver sections were collected for histopathological exam...
International Journal of Pharmacology, 2010
ABSTRACT In this study, Nasturtium officinale R. Br. (watercress), an edible plant from crucifero... more ABSTRACT In this study, Nasturtium officinale R. Br. (watercress), an edible plant from cruciferous plants and its three fractions were screened for their hepatoprotective effects against acetaminophen induced toxicity in rats. The total parts of plant were extracted with alcohol-water and then sequentially fractionated. The total extract and its three fractions namely; petroleum ether; n -butyl alcohol; and aqueous at two doses (50 and 175 mg kg-1) were administrated to animals every 24 h for five successive days. Rats treated as post treatment with the total extract or three fractions at the dose of 175 mg kg-1 did not show any significance change in activity of aminotransferse enzymes (AST and ALT) compared to control. Acetaminophen caused significant hepatocellular damage and increase in serum levels of AST, ALT and LDH in comparison to control group (p<0.001). Post treatment with the total extract (175 mg kg-1) and aqueous fraction (50 mg kg-1) significantly prevented acetaminophen induced rise in serum levels of AST, ALT and LDH. LD50 value of the petroleum fraction was more than 3823 mg kg-1, while the other two fractions and total extract were non-toxic up to 5734 mg kg-1. Histopathological changes of liver induced by acetaminophen were considerably reversed following treatment by aqueous fraction and total extract of N. officinale . The findings of this study showed that N. officinale may play a protective role against acetaminophen-induced hepatotoxicity through maintaining the normal liver functions which further validates the use of N. officinale in Iranian traditional medicine as a hepatoprotective agent.