Quentin pascal | Université Paris-Est Créteil (original) (raw)
Papers by Quentin pascal
npj vaccines, Jun 20, 2024
The characterization of vaccine distribution to relevant tissues after in vivo administration is ... more The characterization of vaccine distribution to relevant tissues after in vivo administration is critical to understanding their mechanisms of action. Vaccines based on mRNA lipid nanoparticles (LNPs) are now being widely considered against infectious diseases and cancer. Here, we used in vivo imaging approaches to compare the trafficking of two LNP formulations encapsulating mRNA following intramuscular administration: DLin-MC3-DMA (MC3) and the recently developed DOG-IM4. The mRNA formulated in DOG-IM4 LNPs persisted at the injection site, whereas mRNA formulated in MC3 LNPs rapidly migrated to the draining lymph nodes. Furthermore, MC3 LNPs induced the fastest increase in blood neutrophil counts after injection and greater inflammation, as shown by IL-1RA, IL-15, CCL-1, and IL-6 concentrations in nonhuman primate sera. These observations highlight the influence of the nature of the LNP on mRNA vaccine distribution and early immune responses.
Molecular Therapy, May 1, 2014
improve pharmacokinetics, these modifi cations are often performed post-selection and require an ... more improve pharmacokinetics, these modifi cations are often performed post-selection and require an iterative process of trial and error to ensure effi cacy. In principle, combinatorial screening can accelerate identifi cation of optimal modifi cations, but such screening can be laborious and many modifi ed nucleotides are incompatible with the diverse starting libraries required of in vitro selection due to low yields during transcription or cDNA synthesis. To bridge this gap, we developed a novel selection scheme, termed "Poly-2' SELEX," in which pre-enriched RNA aptamer libraries are subjected to additional rounds of selection with 2'-modifi ed pyrimidines that confer serum stability. Herein we report on the identifi cation of serum-stable RNA aptamers against HIV reverse transcriptase (HIV RT) using this novel variation of SELEX technology. Pre-enriched libraries originally selected with standard RNA were subjected to three additional rounds of selection in parallel trajectories in which the 2'-hydroxyl of pyrimidines were replaced with 2'-NH 2 , 2'-F, or 2'-O-Methyl functional groups during library transcription. Each trajectory yielded new populations that bound and inhibited the selection target. Next generation sequencing and comparative sequence analysis of these populations revealed enrichment of aptamers within particular modification trajectories that either accommodate or adapt to these various 2'-modifi cations. Primer extension assays confi rmed that these newly identifi ed aptamers retain strong inhibition of HIV RT and they exhibited signifi cantly longer half-lives in serum compared to standard RNA. When coupled with high-throughput sequencing and bioinformatic analysis, Poly-2' SELEX has the capacity to identify existing aptamers suitable for direct delivery while avoiding potentially costly and diffi cult selections de novo or time-consuming ad hoc optimization.
The fight against COVID-19 requires mass vaccination strategies, and vaccines inducing durable cr... more The fight against COVID-19 requires mass vaccination strategies, and vaccines inducing durable cross-protective responses are still needed. Inactivated vaccines have proven lasting efficacy against many pathogens and good safety records. They contain multiple protein antigens that may improve response breadth and can be easily adapted every year to maintain preparedness for future seasonally emerging variants. Here we report the immunogenicity and efficacy of VLA2001 in animal models, the first inactivated whole virus COVID-19 vaccine that has received standard marketing authorization by the European Medicines Agency. VLA2001 formulated with alum and the TLR9 agonist CpG 1018™ adjuvant generated a Th1-biased immune response and serum neutralizing antibodies in BALB/c mice. In non-human primates, two injections of VLA2001 were sufficient to induce specific and polyfunctional T cell responses, predominantly Th1-biased, and high levels of antibodies neutralizing SARS-CoV-2 infection in...
Revue des Maladies Respiratoires, 2022
Nature Communications
The COVID-19 pandemic has exemplified that rigorous evaluation in large animal models is key for ... more The COVID-19 pandemic has exemplified that rigorous evaluation in large animal models is key for translation from promising in vitro results to successful clinical implementation. Among the drugs that have been largely tested in clinical trials but failed so far to bring clear evidence of clinical efficacy is favipiravir, a nucleoside analogue with large spectrum activity against several RNA viruses in vitro and in small animal models. Here, we evaluate the antiviral activity of favipiravir against Zika or SARS-CoV-2 virus in cynomolgus macaques. In both models, high doses of favipiravir are initiated before infection and viral kinetics are evaluated during 7 to 15 days after infection. Favipiravir leads to a statistically significant reduction in plasma Zika viral load compared to untreated animals. However, favipiravir has no effects on SARS-CoV-2 viral kinetics, and 4 treated animals have to be euthanized due to rapid clinical deterioration, suggesting a potential role of favipir...
Bat sarbecovirus BANAL-236 is highly related to SARS-CoV-2 and infects human cells, albeit lackin... more Bat sarbecovirus BANAL-236 is highly related to SARS-CoV-2 and infects human cells, albeit lacking the furin cleavage site in its spike protein. To inform on the origin of SARS-CoV-2, we evaluated the clinical, epidemiological and evolutionary consequences of a potential BANAL-236 spillover into humans using animal models. The virus replicates efficiently and pauci-symptomatically in humanized mice and in macaques, where its tropism is enteric, strongly differing from that of SARS-CoV-2. BANAL-236 infection leads to protection against superinfection by a more virulent strain like Wuhan SARS-CoV-2. Yet we found no evidence of antibodies recognizing bat sarbecoviruses in populations highly exposed to bats, indicating that such infections, if they occur, are rare. Six passages in mice or in human intestinal cells, mimicking putative early spillover events, selected adaptive mutations without appearance of a furin cleavage site and not change in virulence. We thus conclude that the hypo...
Molecular Therapy
The COVID-19 pandemic continues to have devastating consequences on health and economy, even afte... more The COVID-19 pandemic continues to have devastating consequences on health and economy, even after the approval of safe and effective vaccines. Waning immunity, the emergence of variants of concern, breakthrough infections, and lack of global vaccine access and acceptance perpetuate the epidemic. Here, we demonstrate that a single injection of an adenoassociated virus (AAV)-based COVID-19 vaccine elicits at least 17-monthlong neutralizing antibody responses in non-human primates at levels that were previously shown to protect from viral challenge. To improve the scalability of this durable vaccine candidate, we further optimized the vector design for greater potency at a reduced dose in mice and non-human primates. Finally, we show that the platform can be rapidly adapted to other variants of concern to robustly maintain immunogenicity and protect from challenge. In summary, we demonstrate this class of AAV can provide durable immunogenicity, provide protection at dose that is low and scalable, and be adapted readily to novel emerging vaccine antigens thus may provide a potent tool in the ongoing fight against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Clinical and Experimental Immunology, Apr 16, 2021
Summary The impact of treatment on the risk of lymphoma in patients with rheumatoid arthritis (RA... more Summary The impact of treatment on the risk of lymphoma in patients with rheumatoid arthritis (RA) is unclear. Here, we aimed to assess if the risk of lymphoma differs according to the type of tumor necrosis factor inhibitor (TNFi), comparing monoclonal anti-TNF antibodies to the soluble TNF receptor. We used B cell activating factor belonging to the TNF family (BAFF)-transgenic (Tg) mice as a model of autoimmunity-associated lymphoma. Six-month-old BAFF-Tg mice were treated with TNFi for 12 months. Histological examination of the spleen, assessment of the cellular composition of the spleen by flow cytometry and assessment of B cell clonality were performed at euthanasia. Crude mortality and incidence of lymphoma were significantly higher in mice treated with monoclonal anti-TNF antibodies compared to both controls and mice treated with the soluble TNF receptor, even at a high dose. Flow cytometry analysis revealed decreased splenic macrophage infiltration in mice treated with monoclonal anti-TNF antibodies. Overall, this study demonstrates, for the first time, that a very prolonged treatment with monoclonal anti-TNF antibodies increase the risk of lymphoma in B cell-driven autoimmunity. These data suggest a closer monitoring for lymphoma development in patients suffering from B cell-driven autoimmune disease with long-term exposure to monoclonal anti-TNF antibodies.
Long-term neurologic correction in the Pompe disease mice by intrathecal gene therapy. 17. Annual... more Long-term neurologic correction in the Pompe disease mice by intrathecal gene therapy. 17. Annual meeting of the ASGC
![Research paper thumbnail of Computed tomography and [18F]-FDG PET imaging provide additional readouts for COVID-19 pathogenesis and therapies evaluation in non-human primates](https://attachments.academia-assets.com/92571500/thumbnails/1.jpg)
](iScience, 2022
Macaques exposed to SARS-CoV-2 show typical mild COVID-19 lung lesions Infected animals have incr... more Macaques exposed to SARS-CoV-2 show typical mild COVID-19 lung lesions Infected animals have increased metabolism in airways and some lymphatic organs PET and CT imaging provide an additional readout to assess therapy efficacy
Supplemental Material, Combined_supplemental_materials-Rogez_et_al for Expression and Prognostic ... more Supplemental Material, Combined_supplemental_materials-Rogez_et_al for Expression and Prognostic Significance of Neurotrophins and Their Receptors in Canine Mammary Tumors by Bernadette Rogez, Quentin Pascal, Audrey Bobillier, François Machuron, Robert-Alain Toillon, Dominique Tierny, Valérie Chopin and Xuefen Le Bourhis in Veterinary Pathology
Supplemental_table_10.03.19 for Expression and Prognostic Significance of Neurotrophins and Their... more Supplemental_table_10.03.19 for Expression and Prognostic Significance of Neurotrophins and Their Receptors in Canine Mammary Tumors by Bernadette Rogez, Quentin Pascal, Audrey Bobillier, François Machuron, Robert-Alain Toillon, Dominique Tierny, Valérie Chopin and Xuefen Le Bourhis in Veterinary Pathology
Supplemental Material, DS2_VET_10.1177_0300985818813653 for CD44 and CD24 Expression and Prognost... more Supplemental Material, DS2_VET_10.1177_0300985818813653 for CD44 and CD24 Expression and Prognostic Significance in Canine Mammary Tumors by Bernadette Rogez, Quentin Pascal, Audrey Bobillier, François Machuron, Chann Lagadec, Dominique Tierny, Xuefen Le Bourhis, and Valérie Chopin in Veterinary Pathology
Supplemental Material, DS1_VET_10.1177_0300985818813653 for CD44 and CD24 Expression and Prognost... more Supplemental Material, DS1_VET_10.1177_0300985818813653 for CD44 and CD24 Expression and Prognostic Significance in Canine Mammary Tumors by Bernadette Rogez, Quentin Pascal, Audrey Bobillier, François Machuron, Chann Lagadec, Dominique Tierny, Xuefen Le Bourhis, and Valérie Chopin in Veterinary Pathology
Journal of the American Animal Hospital Association, 2019
A 4 mo old female Staffordshire bull terrier puppy was presented with chronic Escherichia coli cy... more A 4 mo old female Staffordshire bull terrier puppy was presented with chronic Escherichia coli cystitis. Ultrasound and cystoscopic examination revealed innumerable, intraluminal, finger-like proliferations arising from the dorsal urinary bladder (UB) wall. Histological examination of mucosal biopsies obtained by cystoscopy was suggestive of granulomatous cystitis. The proliferative lesions were removed surgically and submitted for histological examination. The UB submucosa was heavily infiltrated by macrophages with periodic acid-Schiff–positive cytoplasm exhibiting rare Michaelis-Gutmann bodies, leading to the diagnosis of malakoplakia. The puppy was prescribed with sulfamethoxazole-trimethoprim. The urinary signs disappeared despite the persistent UB wall thickening revealed by abdominal ultrasound. Urine culture performed during the ninth week of treatment showed a persistent infection by E coli resistant to sulfamethoxazole-trimethoprim. The dog was switched to doxycycline but ...
SSRN Electronic Journal, 2018
Open Veterinary Journal, 2019
A 15-year-old neutered female domestic shorthair cat was presented for weight loss, polydipsia/po... more A 15-year-old neutered female domestic shorthair cat was presented for weight loss, polydipsia/polyuria, and lethargy. A large fluctuant mass was palpated in the ventral right cervical region. Biochemistry results were consistent with primary hyperparathyroidism. Parathyroid hormone level in the fluid was higher to that observed in the plasma, consistent with a cystic parathyroid lesion. Right parathyroidectomy and thyroidectomy were performed without complications. Ionized calcium normalized within a few hours. Histopathology yielded a diagnosis of cystic parathyroid adenoma. Follow-up showed complete recovery of clinical signs and normalization of ionized calcium. This case shows an uncommon presentation of feline primary hyperparathyroidism secondary to a cystic parathyroid adenoma and is, to our knowledge, the first case presented with a large palpable mass in which parathyroid hormone concentration was measured. This report highlights the value of selective hormonal analyses of the cystic fluid to confirm the origin of the cystic lesion pre-operatively.
Journal of Veterinary Diagnostic Investigation, 2018
A 6-y-old neutered male ferret ( Mustela putorius furo) was presented because of a 1-mo history o... more A 6-y-old neutered male ferret ( Mustela putorius furo) was presented because of a 1-mo history of progressive weight loss, chronic cough, and hair loss. On clinical examination, the animal was coughing, slightly depressed, moderately hypothermic, and had bilateral epiphora. Thoracic radiography was suggestive of severe multinodular interstitial pneumonia. Abdominal ultrasound examination revealed hepatosplenomegaly and mesenteric and pancreaticoduodenal lymphadenopathy. Fine-needle aspiration of the pancreaticoduodenal lymph node, followed by routine Romanowsky and Ziehl–Neelsen stains, revealed numerous macrophages containing myriad acid-fast bacilli, leading to identification of mycobacteriosis. Autopsy and histologic examination confirmed the presence of disseminated, poorly defined, acid-fast, bacilli-rich granulomas in the pancreaticoduodenal and mesenteric lymph nodes, intestines, and lungs. Destaining of May-Grünwald/Giemsa–stained slides with alcohol, and then restaining wi...
npj vaccines, Jun 20, 2024
The characterization of vaccine distribution to relevant tissues after in vivo administration is ... more The characterization of vaccine distribution to relevant tissues after in vivo administration is critical to understanding their mechanisms of action. Vaccines based on mRNA lipid nanoparticles (LNPs) are now being widely considered against infectious diseases and cancer. Here, we used in vivo imaging approaches to compare the trafficking of two LNP formulations encapsulating mRNA following intramuscular administration: DLin-MC3-DMA (MC3) and the recently developed DOG-IM4. The mRNA formulated in DOG-IM4 LNPs persisted at the injection site, whereas mRNA formulated in MC3 LNPs rapidly migrated to the draining lymph nodes. Furthermore, MC3 LNPs induced the fastest increase in blood neutrophil counts after injection and greater inflammation, as shown by IL-1RA, IL-15, CCL-1, and IL-6 concentrations in nonhuman primate sera. These observations highlight the influence of the nature of the LNP on mRNA vaccine distribution and early immune responses.
Molecular Therapy, May 1, 2014
improve pharmacokinetics, these modifi cations are often performed post-selection and require an ... more improve pharmacokinetics, these modifi cations are often performed post-selection and require an iterative process of trial and error to ensure effi cacy. In principle, combinatorial screening can accelerate identifi cation of optimal modifi cations, but such screening can be laborious and many modifi ed nucleotides are incompatible with the diverse starting libraries required of in vitro selection due to low yields during transcription or cDNA synthesis. To bridge this gap, we developed a novel selection scheme, termed "Poly-2' SELEX," in which pre-enriched RNA aptamer libraries are subjected to additional rounds of selection with 2'-modifi ed pyrimidines that confer serum stability. Herein we report on the identifi cation of serum-stable RNA aptamers against HIV reverse transcriptase (HIV RT) using this novel variation of SELEX technology. Pre-enriched libraries originally selected with standard RNA were subjected to three additional rounds of selection in parallel trajectories in which the 2'-hydroxyl of pyrimidines were replaced with 2'-NH 2 , 2'-F, or 2'-O-Methyl functional groups during library transcription. Each trajectory yielded new populations that bound and inhibited the selection target. Next generation sequencing and comparative sequence analysis of these populations revealed enrichment of aptamers within particular modification trajectories that either accommodate or adapt to these various 2'-modifi cations. Primer extension assays confi rmed that these newly identifi ed aptamers retain strong inhibition of HIV RT and they exhibited signifi cantly longer half-lives in serum compared to standard RNA. When coupled with high-throughput sequencing and bioinformatic analysis, Poly-2' SELEX has the capacity to identify existing aptamers suitable for direct delivery while avoiding potentially costly and diffi cult selections de novo or time-consuming ad hoc optimization.
The fight against COVID-19 requires mass vaccination strategies, and vaccines inducing durable cr... more The fight against COVID-19 requires mass vaccination strategies, and vaccines inducing durable cross-protective responses are still needed. Inactivated vaccines have proven lasting efficacy against many pathogens and good safety records. They contain multiple protein antigens that may improve response breadth and can be easily adapted every year to maintain preparedness for future seasonally emerging variants. Here we report the immunogenicity and efficacy of VLA2001 in animal models, the first inactivated whole virus COVID-19 vaccine that has received standard marketing authorization by the European Medicines Agency. VLA2001 formulated with alum and the TLR9 agonist CpG 1018™ adjuvant generated a Th1-biased immune response and serum neutralizing antibodies in BALB/c mice. In non-human primates, two injections of VLA2001 were sufficient to induce specific and polyfunctional T cell responses, predominantly Th1-biased, and high levels of antibodies neutralizing SARS-CoV-2 infection in...
Revue des Maladies Respiratoires, 2022
Nature Communications
The COVID-19 pandemic has exemplified that rigorous evaluation in large animal models is key for ... more The COVID-19 pandemic has exemplified that rigorous evaluation in large animal models is key for translation from promising in vitro results to successful clinical implementation. Among the drugs that have been largely tested in clinical trials but failed so far to bring clear evidence of clinical efficacy is favipiravir, a nucleoside analogue with large spectrum activity against several RNA viruses in vitro and in small animal models. Here, we evaluate the antiviral activity of favipiravir against Zika or SARS-CoV-2 virus in cynomolgus macaques. In both models, high doses of favipiravir are initiated before infection and viral kinetics are evaluated during 7 to 15 days after infection. Favipiravir leads to a statistically significant reduction in plasma Zika viral load compared to untreated animals. However, favipiravir has no effects on SARS-CoV-2 viral kinetics, and 4 treated animals have to be euthanized due to rapid clinical deterioration, suggesting a potential role of favipir...
Bat sarbecovirus BANAL-236 is highly related to SARS-CoV-2 and infects human cells, albeit lackin... more Bat sarbecovirus BANAL-236 is highly related to SARS-CoV-2 and infects human cells, albeit lacking the furin cleavage site in its spike protein. To inform on the origin of SARS-CoV-2, we evaluated the clinical, epidemiological and evolutionary consequences of a potential BANAL-236 spillover into humans using animal models. The virus replicates efficiently and pauci-symptomatically in humanized mice and in macaques, where its tropism is enteric, strongly differing from that of SARS-CoV-2. BANAL-236 infection leads to protection against superinfection by a more virulent strain like Wuhan SARS-CoV-2. Yet we found no evidence of antibodies recognizing bat sarbecoviruses in populations highly exposed to bats, indicating that such infections, if they occur, are rare. Six passages in mice or in human intestinal cells, mimicking putative early spillover events, selected adaptive mutations without appearance of a furin cleavage site and not change in virulence. We thus conclude that the hypo...
Molecular Therapy
The COVID-19 pandemic continues to have devastating consequences on health and economy, even afte... more The COVID-19 pandemic continues to have devastating consequences on health and economy, even after the approval of safe and effective vaccines. Waning immunity, the emergence of variants of concern, breakthrough infections, and lack of global vaccine access and acceptance perpetuate the epidemic. Here, we demonstrate that a single injection of an adenoassociated virus (AAV)-based COVID-19 vaccine elicits at least 17-monthlong neutralizing antibody responses in non-human primates at levels that were previously shown to protect from viral challenge. To improve the scalability of this durable vaccine candidate, we further optimized the vector design for greater potency at a reduced dose in mice and non-human primates. Finally, we show that the platform can be rapidly adapted to other variants of concern to robustly maintain immunogenicity and protect from challenge. In summary, we demonstrate this class of AAV can provide durable immunogenicity, provide protection at dose that is low and scalable, and be adapted readily to novel emerging vaccine antigens thus may provide a potent tool in the ongoing fight against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Clinical and Experimental Immunology, Apr 16, 2021
Summary The impact of treatment on the risk of lymphoma in patients with rheumatoid arthritis (RA... more Summary The impact of treatment on the risk of lymphoma in patients with rheumatoid arthritis (RA) is unclear. Here, we aimed to assess if the risk of lymphoma differs according to the type of tumor necrosis factor inhibitor (TNFi), comparing monoclonal anti-TNF antibodies to the soluble TNF receptor. We used B cell activating factor belonging to the TNF family (BAFF)-transgenic (Tg) mice as a model of autoimmunity-associated lymphoma. Six-month-old BAFF-Tg mice were treated with TNFi for 12 months. Histological examination of the spleen, assessment of the cellular composition of the spleen by flow cytometry and assessment of B cell clonality were performed at euthanasia. Crude mortality and incidence of lymphoma were significantly higher in mice treated with monoclonal anti-TNF antibodies compared to both controls and mice treated with the soluble TNF receptor, even at a high dose. Flow cytometry analysis revealed decreased splenic macrophage infiltration in mice treated with monoclonal anti-TNF antibodies. Overall, this study demonstrates, for the first time, that a very prolonged treatment with monoclonal anti-TNF antibodies increase the risk of lymphoma in B cell-driven autoimmunity. These data suggest a closer monitoring for lymphoma development in patients suffering from B cell-driven autoimmune disease with long-term exposure to monoclonal anti-TNF antibodies.
Long-term neurologic correction in the Pompe disease mice by intrathecal gene therapy. 17. Annual... more Long-term neurologic correction in the Pompe disease mice by intrathecal gene therapy. 17. Annual meeting of the ASGC
iScience, 2022
Macaques exposed to SARS-CoV-2 show typical mild COVID-19 lung lesions Infected animals have incr... more Macaques exposed to SARS-CoV-2 show typical mild COVID-19 lung lesions Infected animals have increased metabolism in airways and some lymphatic organs PET and CT imaging provide an additional readout to assess therapy efficacy
Supplemental Material, Combined_supplemental_materials-Rogez_et_al for Expression and Prognostic ... more Supplemental Material, Combined_supplemental_materials-Rogez_et_al for Expression and Prognostic Significance of Neurotrophins and Their Receptors in Canine Mammary Tumors by Bernadette Rogez, Quentin Pascal, Audrey Bobillier, François Machuron, Robert-Alain Toillon, Dominique Tierny, Valérie Chopin and Xuefen Le Bourhis in Veterinary Pathology
Supplemental_table_10.03.19 for Expression and Prognostic Significance of Neurotrophins and Their... more Supplemental_table_10.03.19 for Expression and Prognostic Significance of Neurotrophins and Their Receptors in Canine Mammary Tumors by Bernadette Rogez, Quentin Pascal, Audrey Bobillier, François Machuron, Robert-Alain Toillon, Dominique Tierny, Valérie Chopin and Xuefen Le Bourhis in Veterinary Pathology
Supplemental Material, DS2_VET_10.1177_0300985818813653 for CD44 and CD24 Expression and Prognost... more Supplemental Material, DS2_VET_10.1177_0300985818813653 for CD44 and CD24 Expression and Prognostic Significance in Canine Mammary Tumors by Bernadette Rogez, Quentin Pascal, Audrey Bobillier, François Machuron, Chann Lagadec, Dominique Tierny, Xuefen Le Bourhis, and Valérie Chopin in Veterinary Pathology
Supplemental Material, DS1_VET_10.1177_0300985818813653 for CD44 and CD24 Expression and Prognost... more Supplemental Material, DS1_VET_10.1177_0300985818813653 for CD44 and CD24 Expression and Prognostic Significance in Canine Mammary Tumors by Bernadette Rogez, Quentin Pascal, Audrey Bobillier, François Machuron, Chann Lagadec, Dominique Tierny, Xuefen Le Bourhis, and Valérie Chopin in Veterinary Pathology
Journal of the American Animal Hospital Association, 2019
A 4 mo old female Staffordshire bull terrier puppy was presented with chronic Escherichia coli cy... more A 4 mo old female Staffordshire bull terrier puppy was presented with chronic Escherichia coli cystitis. Ultrasound and cystoscopic examination revealed innumerable, intraluminal, finger-like proliferations arising from the dorsal urinary bladder (UB) wall. Histological examination of mucosal biopsies obtained by cystoscopy was suggestive of granulomatous cystitis. The proliferative lesions were removed surgically and submitted for histological examination. The UB submucosa was heavily infiltrated by macrophages with periodic acid-Schiff–positive cytoplasm exhibiting rare Michaelis-Gutmann bodies, leading to the diagnosis of malakoplakia. The puppy was prescribed with sulfamethoxazole-trimethoprim. The urinary signs disappeared despite the persistent UB wall thickening revealed by abdominal ultrasound. Urine culture performed during the ninth week of treatment showed a persistent infection by E coli resistant to sulfamethoxazole-trimethoprim. The dog was switched to doxycycline but ...
SSRN Electronic Journal, 2018
Open Veterinary Journal, 2019
A 15-year-old neutered female domestic shorthair cat was presented for weight loss, polydipsia/po... more A 15-year-old neutered female domestic shorthair cat was presented for weight loss, polydipsia/polyuria, and lethargy. A large fluctuant mass was palpated in the ventral right cervical region. Biochemistry results were consistent with primary hyperparathyroidism. Parathyroid hormone level in the fluid was higher to that observed in the plasma, consistent with a cystic parathyroid lesion. Right parathyroidectomy and thyroidectomy were performed without complications. Ionized calcium normalized within a few hours. Histopathology yielded a diagnosis of cystic parathyroid adenoma. Follow-up showed complete recovery of clinical signs and normalization of ionized calcium. This case shows an uncommon presentation of feline primary hyperparathyroidism secondary to a cystic parathyroid adenoma and is, to our knowledge, the first case presented with a large palpable mass in which parathyroid hormone concentration was measured. This report highlights the value of selective hormonal analyses of the cystic fluid to confirm the origin of the cystic lesion pre-operatively.
Journal of Veterinary Diagnostic Investigation, 2018
A 6-y-old neutered male ferret ( Mustela putorius furo) was presented because of a 1-mo history o... more A 6-y-old neutered male ferret ( Mustela putorius furo) was presented because of a 1-mo history of progressive weight loss, chronic cough, and hair loss. On clinical examination, the animal was coughing, slightly depressed, moderately hypothermic, and had bilateral epiphora. Thoracic radiography was suggestive of severe multinodular interstitial pneumonia. Abdominal ultrasound examination revealed hepatosplenomegaly and mesenteric and pancreaticoduodenal lymphadenopathy. Fine-needle aspiration of the pancreaticoduodenal lymph node, followed by routine Romanowsky and Ziehl–Neelsen stains, revealed numerous macrophages containing myriad acid-fast bacilli, leading to identification of mycobacteriosis. Autopsy and histologic examination confirmed the presence of disseminated, poorly defined, acid-fast, bacilli-rich granulomas in the pancreaticoduodenal and mesenteric lymph nodes, intestines, and lungs. Destaining of May-Grünwald/Giemsa–stained slides with alcohol, and then restaining wi...