Richard Arnolin | Paris Sud XI University (original) (raw)

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Papers by Richard Arnolin

Research paper thumbnail of Kont a Ziyanm. Le roman des collections d'Ignames de l'INRA Antilles-Guyane

Photo de couverture : tubercule aérien de Dioscorea bulbifera. Henri Stehlé : directeur du centre... more Photo de couverture : tubercule aérien de Dioscorea bulbifera. Henri Stehlé : directeur du centre. Georges Ano, Richard Arnolin, Lucien Degras et Dalila Petro : chercheurs en génétique et amélioration des plantes. Franciane Gamiette : ingénieur en génétique et amélioration des plantes. Mustapha Bousalem et Marie Umber : chercheurs en virologie. Denis Cornet : chercheur Cirad en agronomie. Christelle Dragyn, Eric Francius, Jean Gélabale , David Lange, René Poitout : techniciens en expérimentation végétale. Suzia Gélébale : technicienne en culture in vitro. Rose-Marie Gomez : technicienne en biologie moléculaire. Franciane Nuissier : informaticienne. Claudie Pavis : directrice du CRB Plantes tropicales.

Research paper thumbnail of Introduction et observation d'ignames d'Océanie dans les Antilles Françaises

Research paper thumbnail of La culture in vitro au service de l'amélioration de l'igname

HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific re... more HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers.

Research paper thumbnail of Bouturage in vitro, en vue de la production de plants chez l'igname Dioscorea Sp. L

Dans le present travail les possibilites de multiplication par bouturage in vitro, de trois culti... more Dans le present travail les possibilites de multiplication par bouturage in vitro, de trois cultivars d’igname presentant un grand interet economique ont ete etudiees, et le comportement des vitroplantules ainsi obtenues a ete observe. Les explants primaires doivent etre preleves sur des parties jeunes de plantes conduites en serre. Les auxines sont favorables a l’enracinement de boutures ; une cycokinine comme la benzyl adenine est plutot favorable a la caulogenese. D. Cayenensis rotundata, c. V. V17/2, donne le meilleur coefficient de multiplication, sur le milieu 169, derive du milieu M. S. Par C. Martin, soit 164 000 en 12 mois ; mais c’est aussi le cultivar qui presente le plus de difficultes au moment du transfert au champ. L’allongement de la longueur du jour permet la production de plants de V17/2, avec un coefficient de multiplication theorique de 484 000 sur deux ans. D. Trifida (c. V. INRA 5-20) et D. Alata (c. V. Belep) sont plus rustiques, quant a leur transfert au cham...

Research paper thumbnail of Microsatellite segregation analysis and cytogenetic evidence for tetrasomic inheritance in the American yam Dioscorea trifida and a new basic chromosome number in the Dioscoreae

Theoretical and Applied Genetics, 2006

Despite the economic and cultural importance of the indigenous &a... more Despite the economic and cultural importance of the indigenous "Amerindian" yam Dioscorea trifida, very little is known about their origin, phylogeny, diversity and genetics. Consequently, conventional breeding efforts for the selection of D. trifida genotypes resistant to potyviruses which are directly involved in the regression of this species have been seriously limited. Our objective of this paper is to contribute to the clarification of the cytogenetic status, i.e., inheritance and chromosome number. Our results provide genetic evidence supporting tetrasomic behaviour of the genome of D. trifida based on chromosomal segregation pattern analysis using eight SSRs markers in three different crosses. This is the first reliable evidence of an autopolyploid species in the genus Dioscorea. The second major result in this study is the revealing of a new base chromosome number in the botanical section Macrogynodium to which D. trifida belongs. To date, our assumptions about the ploidy level of yams are based on the observations that the basic chromosome number is 10 or 9, and D. trifida was described as octoploid. The chromosome number of D. trifida accessions was also assessed using somatic chromosomic count techniques. Flow cytometry did not show significant variation of 2C DNA content among 80 accessions indicating homogeneity of the ploidy level of the cultivated D. trifida. This suggests that autotetraploidy is well established as well as the rule for the cultivated pool of D. trifida, even if the direct diploid ancestor remains to be identified. The data presented in this paper are significant and important for the effective breeding and conservation of the species and for elucidating the phylogeny and the origins of the yam and the evolution of the genus Dioscorea.

Research paper thumbnail of Microsatellite segregation analysis and cytogenetic evidence for tetrasomic inheritance in the American yam Dioscorea trifida and a new basic chromosome number in the Dioscoreae

Theoretical and Applied Genetics - THEOR APPL GENET, 2006

Despite the economic and cultural importance of the indigenous “Amerindian” yam Dioscorea trifida... more Despite the economic and cultural importance of the indigenous “Amerindian” yam Dioscorea trifida, very little is known about their origin, phylogeny, diversity and genetics. Consequently, conventional breeding efforts for the selection of D. trifida genotypes resistant to potyviruses which are directly involved in the regression of this species have been seriously limited. Our objective of this paper is to contribute to the clarification of the cytogenetic status, i.e., inheritance and chromosome number. Our results provide genetic evidence supporting tetrasomic behaviour of the genome of D. trifida based on chromosomal segregation pattern analysis using eight SSRs markers in three different crosses. This is the first reliable evidence of an autopolyploid species in the genus Dioscorea. The second major result in this study is the revealing of a new base chromosome number in the botanical section Macrogynodium to which D. trifida belongs. To date, our assumptions about the ploidy l...

Research paper thumbnail of Microsatellite segregation analysis and cytogenetic evidence for tetrasomic inheritance in the American yam Dioscorea trifida and a new basic chromosome number in the Dioscoreae

Theoretical and Applied Genetics, 2006

Despite the economic and cultural importance of the indigenous &a... more Despite the economic and cultural importance of the indigenous "Amerindian" yam Dioscorea trifida, very little is known about their origin, phylogeny, diversity and genetics. Consequently, conventional breeding efforts for the selection of D. trifida genotypes resistant to potyviruses which are directly involved in the regression of this species have been seriously limited. Our objective of this paper is to contribute to the clarification of the cytogenetic status, i.e., inheritance and chromosome number. Our results provide genetic evidence supporting tetrasomic behaviour of the genome of D. trifida based on chromosomal segregation pattern analysis using eight SSRs markers in three different crosses. This is the first reliable evidence of an autopolyploid species in the genus Dioscorea. The second major result in this study is the revealing of a new base chromosome number in the botanical section Macrogynodium to which D. trifida belongs. To date, our assumptions about the ploidy level of yams are based on the observations that the basic chromosome number is 10 or 9, and D. trifida was described as octoploid. The chromosome number of D. trifida accessions was also assessed using somatic chromosomic count techniques. Flow cytometry did not show significant variation of 2C DNA content among 80 accessions indicating homogeneity of the ploidy level of the cultivated D. trifida. This suggests that autotetraploidy is well established as well as the rule for the cultivated pool of D. trifida, even if the direct diploid ancestor remains to be identified. The data presented in this paper are significant and important for the effective breeding and conservation of the species and for elucidating the phylogeny and the origins of the yam and the evolution of the genus Dioscorea.

Research paper thumbnail of Kont a Ziyanm. Le roman des collections d'Ignames de l'INRA Antilles-Guyane

Photo de couverture : tubercule aérien de Dioscorea bulbifera. Henri Stehlé : directeur du centre... more Photo de couverture : tubercule aérien de Dioscorea bulbifera. Henri Stehlé : directeur du centre. Georges Ano, Richard Arnolin, Lucien Degras et Dalila Petro : chercheurs en génétique et amélioration des plantes. Franciane Gamiette : ingénieur en génétique et amélioration des plantes. Mustapha Bousalem et Marie Umber : chercheurs en virologie. Denis Cornet : chercheur Cirad en agronomie. Christelle Dragyn, Eric Francius, Jean Gélabale , David Lange, René Poitout : techniciens en expérimentation végétale. Suzia Gélébale : technicienne en culture in vitro. Rose-Marie Gomez : technicienne en biologie moléculaire. Franciane Nuissier : informaticienne. Claudie Pavis : directrice du CRB Plantes tropicales.

Research paper thumbnail of Introduction et observation d'ignames d'Océanie dans les Antilles Françaises

Research paper thumbnail of La culture in vitro au service de l'amélioration de l'igname

HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific re... more HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers.

Research paper thumbnail of Bouturage in vitro, en vue de la production de plants chez l'igname Dioscorea Sp. L

Dans le present travail les possibilites de multiplication par bouturage in vitro, de trois culti... more Dans le present travail les possibilites de multiplication par bouturage in vitro, de trois cultivars d’igname presentant un grand interet economique ont ete etudiees, et le comportement des vitroplantules ainsi obtenues a ete observe. Les explants primaires doivent etre preleves sur des parties jeunes de plantes conduites en serre. Les auxines sont favorables a l’enracinement de boutures ; une cycokinine comme la benzyl adenine est plutot favorable a la caulogenese. D. Cayenensis rotundata, c. V. V17/2, donne le meilleur coefficient de multiplication, sur le milieu 169, derive du milieu M. S. Par C. Martin, soit 164 000 en 12 mois ; mais c’est aussi le cultivar qui presente le plus de difficultes au moment du transfert au champ. L’allongement de la longueur du jour permet la production de plants de V17/2, avec un coefficient de multiplication theorique de 484 000 sur deux ans. D. Trifida (c. V. INRA 5-20) et D. Alata (c. V. Belep) sont plus rustiques, quant a leur transfert au cham...

Research paper thumbnail of Microsatellite segregation analysis and cytogenetic evidence for tetrasomic inheritance in the American yam Dioscorea trifida and a new basic chromosome number in the Dioscoreae

Theoretical and Applied Genetics, 2006

Despite the economic and cultural importance of the indigenous &a... more Despite the economic and cultural importance of the indigenous "Amerindian" yam Dioscorea trifida, very little is known about their origin, phylogeny, diversity and genetics. Consequently, conventional breeding efforts for the selection of D. trifida genotypes resistant to potyviruses which are directly involved in the regression of this species have been seriously limited. Our objective of this paper is to contribute to the clarification of the cytogenetic status, i.e., inheritance and chromosome number. Our results provide genetic evidence supporting tetrasomic behaviour of the genome of D. trifida based on chromosomal segregation pattern analysis using eight SSRs markers in three different crosses. This is the first reliable evidence of an autopolyploid species in the genus Dioscorea. The second major result in this study is the revealing of a new base chromosome number in the botanical section Macrogynodium to which D. trifida belongs. To date, our assumptions about the ploidy level of yams are based on the observations that the basic chromosome number is 10 or 9, and D. trifida was described as octoploid. The chromosome number of D. trifida accessions was also assessed using somatic chromosomic count techniques. Flow cytometry did not show significant variation of 2C DNA content among 80 accessions indicating homogeneity of the ploidy level of the cultivated D. trifida. This suggests that autotetraploidy is well established as well as the rule for the cultivated pool of D. trifida, even if the direct diploid ancestor remains to be identified. The data presented in this paper are significant and important for the effective breeding and conservation of the species and for elucidating the phylogeny and the origins of the yam and the evolution of the genus Dioscorea.

Research paper thumbnail of Microsatellite segregation analysis and cytogenetic evidence for tetrasomic inheritance in the American yam Dioscorea trifida and a new basic chromosome number in the Dioscoreae

Theoretical and Applied Genetics - THEOR APPL GENET, 2006

Despite the economic and cultural importance of the indigenous “Amerindian” yam Dioscorea trifida... more Despite the economic and cultural importance of the indigenous “Amerindian” yam Dioscorea trifida, very little is known about their origin, phylogeny, diversity and genetics. Consequently, conventional breeding efforts for the selection of D. trifida genotypes resistant to potyviruses which are directly involved in the regression of this species have been seriously limited. Our objective of this paper is to contribute to the clarification of the cytogenetic status, i.e., inheritance and chromosome number. Our results provide genetic evidence supporting tetrasomic behaviour of the genome of D. trifida based on chromosomal segregation pattern analysis using eight SSRs markers in three different crosses. This is the first reliable evidence of an autopolyploid species in the genus Dioscorea. The second major result in this study is the revealing of a new base chromosome number in the botanical section Macrogynodium to which D. trifida belongs. To date, our assumptions about the ploidy l...

Research paper thumbnail of Microsatellite segregation analysis and cytogenetic evidence for tetrasomic inheritance in the American yam Dioscorea trifida and a new basic chromosome number in the Dioscoreae

Theoretical and Applied Genetics, 2006

Despite the economic and cultural importance of the indigenous &a... more Despite the economic and cultural importance of the indigenous "Amerindian" yam Dioscorea trifida, very little is known about their origin, phylogeny, diversity and genetics. Consequently, conventional breeding efforts for the selection of D. trifida genotypes resistant to potyviruses which are directly involved in the regression of this species have been seriously limited. Our objective of this paper is to contribute to the clarification of the cytogenetic status, i.e., inheritance and chromosome number. Our results provide genetic evidence supporting tetrasomic behaviour of the genome of D. trifida based on chromosomal segregation pattern analysis using eight SSRs markers in three different crosses. This is the first reliable evidence of an autopolyploid species in the genus Dioscorea. The second major result in this study is the revealing of a new base chromosome number in the botanical section Macrogynodium to which D. trifida belongs. To date, our assumptions about the ploidy level of yams are based on the observations that the basic chromosome number is 10 or 9, and D. trifida was described as octoploid. The chromosome number of D. trifida accessions was also assessed using somatic chromosomic count techniques. Flow cytometry did not show significant variation of 2C DNA content among 80 accessions indicating homogeneity of the ploidy level of the cultivated D. trifida. This suggests that autotetraploidy is well established as well as the rule for the cultivated pool of D. trifida, even if the direct diploid ancestor remains to be identified. The data presented in this paper are significant and important for the effective breeding and conservation of the species and for elucidating the phylogeny and the origins of the yam and the evolution of the genus Dioscorea.