Saul Emmanuel Torres Garcia | Universidad Autónoma de Aguascalientes (original) (raw)

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Papers by Saul Emmanuel Torres Garcia

Since its inception, the animal cell culture in the twentieth century is related to its usefulnes... more Since its inception, the animal cell culture in the twentieth century is related to its usefulness in research. Early methods such as Harrison culture of the inverted drop, Carrel’s and Lindbergh’s innovations made with the introduction of the use of the infusion pump, the culture of HeLa cells and vaccine design using animal cell cultures are approach which revolutioned the implementation and study of the cell culture. One of the important steps in this technique is the selection of the culture media, which provides the physical and chemical conditions close to those occurring in the natural environment for cell growth, which are crucial for the adhesion, proliferation and cell survival in vitro. In this review, the essential aspects that define this technique are shown, offering a historical overview of the most relevant events and the current prospect in the development of the cell culture, that have been enabled the progress in both, basic and applied research.

ISIJ International, 2013

The main aims of the present research is the study of steel flow under temperature gradient to un... more The main aims of the present research is the study of steel flow under temperature gradient to understand the convective effects on the flow patterns inside the mold and its effects on the shell growth kinetics under more realistic conditions. In order to achieve this, a non-isothermal mathematical model is developed based on the Navier-Stokes equations together with the k-ε turbulence model, the volume of fluid model to solve the multiphase system air-slag-steel and a solidification model. Comparing isothermal and non-isothermal results, it is observed that the buoyancy forces are large enough to modify radically the lower recirculation flows inducing shorter and upwards streams; however, the upper recirculation flows do not show strong changes. Shell growth does not necessarily follow a steady parabolic growth and it is more dependent on the washing effects of convective steel streams. Therefore, shell thickness reports heterogeneous and irregular magnitudes through the four faces of the slab. In addition, mold curvature provides uneven shell growth in the inner side of the slab while in the outer side the shell thickness observes a more regular growth. Shell thickness is irregular and discontinuous all around the upper periphery of the slab; therefore, this region is very sensitive to cracking. Finally, the numerical results for liquid steel solidification are compared to published results of shell growth showing a very good qualitative agreement.

Since its inception, the animal cell culture in the twentieth century is related to its usefulnes... more Since its inception, the animal cell culture in the twentieth century is related to its usefulness in research. Early methods such as Harrison culture of the inverted drop, Carrel’s and Lindbergh’s innovations made with the introduction of the use of the infusion pump, the culture of HeLa cells and vaccine design using animal cell cultures are approach which revolutioned the implementation and study of the cell culture. One of the important steps in this technique is the selection of the culture media, which provides the physical and chemical conditions close to those occurring in the natural environment for cell growth, which are crucial for the adhesion, proliferation and cell survival in vitro. In this review, the essential aspects that define this technique are shown, offering a historical overview of the most relevant events and the current prospect in the development of the cell culture, that have been enabled the progress in both, basic and applied research.

Since its inception, the animal cell culture in the twentieth century is related to its usefulnes... more Since its inception, the animal cell culture in the twentieth century is related to its usefulness in research. Early methods such as Harrison culture of the inverted drop, Carrel’s and Lindbergh’s innovations made with the introduction of the use of the infusion pump, the culture of HeLa cells and vaccine design using animal cell cultures are approach which revolutioned the implementation and study of the cell culture. One of the important steps in this technique is the selection of the culture media, which provides the physical and chemical conditions close to those occurring in the natural environment for cell growth, which are crucial for the adhesion, proliferation and cell survival in vitro. In this review, the essential aspects that define this technique are shown, offering a historical overview of the most relevant events and the current prospect in the development of the cell culture, that have been enabled the progress in both, basic and applied research.

Cells are able to respond to stress stimuli through the expression of proteins that compensate fo... more Cells are able to respond to stress stimuli through the expression of proteins that
compensate for or seek to return the cell to its functional status. Among the most
studied proteins in relation to stress are heat shock proteins(HSPs) or chaperones;
these proteins were originally discovered as induced proteins that facilitate proper
folding of proteins with non-native conformations. HSPs are a set of highly
conserved proteins that are present in all types of cells, from microbes to mammals.
In bacteria, chaperones and proteases regulate cell activity under stress conditions. In
echinoderms, aquatic invertebrates and fish stress proteins response mechanisms are
associated with the expression of chaperones that protect them of temperature
changes and oxidative stress. In mammalian cells, there is the expression of antistress
mechanisms that are also associated with certain physiological conditions such
as mitosis and maintaining the cell under homeostatic conditions. In plants, one of the
conditions most studied is the response to osmotic stress, leading to the synthesis of
membrane transporter systems that compensate the water flow and at the same time,
over expressing intracellular mechanisms associated with enzymes that contribute to
decrease the production of free oxygen radicals. In general, cells of all organisms are
capable of initiating regulatory mechanisms that contribute a reduction in cell injury
that can result in loss of homeostasis. When this mechanisms decline because aging
or faulty metabolism, disease occur. This finding allows to use them as a therapeutic
alternative for aging diseases as Alzheimer and cancer between others.

Since its inception, the animal cell culture in the twentieth century is related to its usefulnes... more Since its inception, the animal cell culture in the twentieth century is related to its usefulness in research. Early methods such as Harrison culture of the inverted drop, Carrel’s and Lindbergh’s innovations made with the introduction of the use of the infusion pump, the culture of HeLa cells and vaccine design using animal cell cultures are approach which revolutioned the implementation and study of the cell culture. One of the important steps in this technique is the selection of the culture media, which provides the physical and chemical conditions close to those occurring in the natural environment for cell growth, which are crucial for the adhesion, proliferation and cell survival in vitro. In this review, the essential aspects that define this technique are shown, offering a historical overview of the most relevant events and the current prospect in the development of the cell culture, that have been enabled the progress in both, basic and applied research.

ISIJ International, 2013

The main aims of the present research is the study of steel flow under temperature gradient to un... more The main aims of the present research is the study of steel flow under temperature gradient to understand the convective effects on the flow patterns inside the mold and its effects on the shell growth kinetics under more realistic conditions. In order to achieve this, a non-isothermal mathematical model is developed based on the Navier-Stokes equations together with the k-ε turbulence model, the volume of fluid model to solve the multiphase system air-slag-steel and a solidification model. Comparing isothermal and non-isothermal results, it is observed that the buoyancy forces are large enough to modify radically the lower recirculation flows inducing shorter and upwards streams; however, the upper recirculation flows do not show strong changes. Shell growth does not necessarily follow a steady parabolic growth and it is more dependent on the washing effects of convective steel streams. Therefore, shell thickness reports heterogeneous and irregular magnitudes through the four faces of the slab. In addition, mold curvature provides uneven shell growth in the inner side of the slab while in the outer side the shell thickness observes a more regular growth. Shell thickness is irregular and discontinuous all around the upper periphery of the slab; therefore, this region is very sensitive to cracking. Finally, the numerical results for liquid steel solidification are compared to published results of shell growth showing a very good qualitative agreement.

Since its inception, the animal cell culture in the twentieth century is related to its usefulnes... more Since its inception, the animal cell culture in the twentieth century is related to its usefulness in research. Early methods such as Harrison culture of the inverted drop, Carrel’s and Lindbergh’s innovations made with the introduction of the use of the infusion pump, the culture of HeLa cells and vaccine design using animal cell cultures are approach which revolutioned the implementation and study of the cell culture. One of the important steps in this technique is the selection of the culture media, which provides the physical and chemical conditions close to those occurring in the natural environment for cell growth, which are crucial for the adhesion, proliferation and cell survival in vitro. In this review, the essential aspects that define this technique are shown, offering a historical overview of the most relevant events and the current prospect in the development of the cell culture, that have been enabled the progress in both, basic and applied research.

Since its inception, the animal cell culture in the twentieth century is related to its usefulnes... more Since its inception, the animal cell culture in the twentieth century is related to its usefulness in research. Early methods such as Harrison culture of the inverted drop, Carrel’s and Lindbergh’s innovations made with the introduction of the use of the infusion pump, the culture of HeLa cells and vaccine design using animal cell cultures are approach which revolutioned the implementation and study of the cell culture. One of the important steps in this technique is the selection of the culture media, which provides the physical and chemical conditions close to those occurring in the natural environment for cell growth, which are crucial for the adhesion, proliferation and cell survival in vitro. In this review, the essential aspects that define this technique are shown, offering a historical overview of the most relevant events and the current prospect in the development of the cell culture, that have been enabled the progress in both, basic and applied research.

Cells are able to respond to stress stimuli through the expression of proteins that compensate fo... more Cells are able to respond to stress stimuli through the expression of proteins that
compensate for or seek to return the cell to its functional status. Among the most
studied proteins in relation to stress are heat shock proteins(HSPs) or chaperones;
these proteins were originally discovered as induced proteins that facilitate proper
folding of proteins with non-native conformations. HSPs are a set of highly
conserved proteins that are present in all types of cells, from microbes to mammals.
In bacteria, chaperones and proteases regulate cell activity under stress conditions. In
echinoderms, aquatic invertebrates and fish stress proteins response mechanisms are
associated with the expression of chaperones that protect them of temperature
changes and oxidative stress. In mammalian cells, there is the expression of antistress
mechanisms that are also associated with certain physiological conditions such
as mitosis and maintaining the cell under homeostatic conditions. In plants, one of the
conditions most studied is the response to osmotic stress, leading to the synthesis of
membrane transporter systems that compensate the water flow and at the same time,
over expressing intracellular mechanisms associated with enzymes that contribute to
decrease the production of free oxygen radicals. In general, cells of all organisms are
capable of initiating regulatory mechanisms that contribute a reduction in cell injury
that can result in loss of homeostasis. When this mechanisms decline because aging
or faulty metabolism, disease occur. This finding allows to use them as a therapeutic
alternative for aging diseases as Alzheimer and cancer between others.