Ivone Giffard-Mena | Universidad Autonoma de Baja California (original) (raw)

Papers by Ivone Giffard-Mena

Applied and environmental microbiology, May 21, 2024

Diseases of aquatic organisms, Apr 11, 2024

Integrative And Comparative Biology

Synopsis Totoaba macdonaldi is an endangered endemic fish of the Gulf of California. Overexploita... more Synopsis Totoaba macdonaldi is an endangered endemic fish of the Gulf of California. Overexploitation resulted in the Mexican government banning the fishing of this species in 1975, and it being listed as endangered. However, the species is still subject to illegal fishing. Despite its conservation status, little is known about totoaba biology. The present study aimed to implement, for the first time, a metabarcoding protocol to describe the totoaba diet. Four wild totoaba individuals, seized by Mexican law enforcement agents, were dissected, and their stomach contents were collected. Three representative amplicon libraries were generated for cephalopods, chordates, and eukaryotes. After sequencing, 18 different taxa were identified, of which 11 species were recognized as prey. The totoaba were found to have consumed Pacific anchovy (Cetengraulis mysticetus), flathead grey mullet (Mugil cephalus), bigeye croaker (Micropogonias megalops), northern anchovy (Engraulis mordax), ocean wh...

$Research paper thumbnail of Transcriptomic response of the Crassostrea virginica gonad after exposure to a water-accommodation fraction of hydrocarbons and the potential implications in reproduction$

Marine Genomics, 2018

The Crassostrea virginica oyster has biological and economic importance in the Gulf of Mexico, an... more The Crassostrea virginica oyster has biological and economic importance in the Gulf of Mexico, an area with a high extraction and production of hydrocarbons. Exposure to hydrocarbons affects the reproductive processes in bivalves. In C. virginica, the effect of hydrocarbons on the gonad of the undifferentiated organism has not been evaluated to determine the possible damage during the maturation process. To evaluate this effect, RNA-seq data was generated from C. virginica gonads exposed to a 200 μg/L of hydrocarbons at different exposure times (7, 14 and 21 days) and a control treatment (without hydrocarbons). The analysis of the gonad transcriptome showed the negative effect of hydrocarbons on maturation, with a sub-expression of 22 genes involved in different stages of this process. Additionally, genes in the immune system were down-regulated, which may indicate that exposure to hydrocarbons causes immunosuppression in bivalves. A group of oxidative stress genes was also reduced. These data contribute to a better understanding of the effect of hydrocarbons on the reproductive process in bivalves and, at the same time, allow us to identify possible biomarkers associated with hydrocarbon contamination in the gonad of C. virginica.

The FASEB Journal, Mar 1, 2008

Development Growth & Differentiation, Apr 1, 2006

The ontogeny of the digestive tract (DT) and of Na + /K +-ATPase localization was investigated du... more The ontogeny of the digestive tract (DT) and of Na + /K +-ATPase localization was investigated during the early postembryonic development (from yolk sac larva to juvenile) of the euryhaline teleost Dicentrarchus labrax reared at two salinities: seawater and diluted seawater. Histology, electron microscopy and immunocytochemistry were used to determine the presence and differentiation of ion transporting cells. At hatching, the DT is an undifferentiated straight tube over the yolk sac. At the mouth opening (day 5), it comprises six segments: buccopharynx, esophagus, stomach, anterior intestine, posterior intestine and rectum, well differentiated at the juvenile stage (day 72). The enterocytes displayed ultrastructural features similar to those of mitochondria-rich cells known to be involved in active ion transport. At hatching, ion transporting cells lining the intestine and the rectum exhibited a Na + /K +-ATPase activity which increased mainly after the larva/juvenile (20 mm) metamorphic transition. The immunofluorescence intensity was dependent upon the stage of development of the gut as well as on the histological configuration of the analyzed segment. The appearance and distribution of enteric ionocytes and the implication of the DT in osmoregulation are discussed.

Comparative Biochemistry and Physiology A-molecular & Integrative Physiology, Oct 1, 2007

Euryhaline fish possess the ability to compensate for environmental salinity changes through hydr... more Euryhaline fish possess the ability to compensate for environmental salinity changes through hydro-mineral regulation. A number of proteins have been studied in order to understand water and ion exchanges, known as fish osmoregulation. Sea-bass (Dicentrarchus labrax) cDNA sequences encoding a homologue of mammalian aquaporin (termed AQP1) and a homologue of mammalian aquaglyceroporin (termed AQP3) have been isolated and sequenced. The aquaporin amino acid sequences share respectively more than 60% and 65% identity with other known aquaporins. We have shown that salinity influences aquaporin expression levels in the gill, kidney and digestive tract, the main osmoregulatory organs. AQP1 may have a major osmoregulatory role in water transport in kidney and gut in SW-acclimated fish, whereas AQP3 could be implicated in gill water transport in FW-acclimated fish.

Diseases of Aquatic Organisms

White spot syndrome virus (WSSV) infects several economically important aquaculture species, and ... more White spot syndrome virus (WSSV) infects several economically important aquaculture species, and has caused significant losses to the industry. This virus belongs to the Nimaviridae family and has a dsDNA genome ranging between 257 and 309 kb (more than 20 isolate genomes have been fully sequenced and published to date). Multiple routes of infection could be the cause of the high virulence and mortality rates detected in shrimp species. Particularly in Penaeus vannamei, differences in isolate virulence have been observed, along with controversy over whether deletions or insertions are associated with virulence gain or loss. The pathogenicity of 3 isolates from 3 localities in Mexico (2 from Sinaloa: ‘CIAD’ and ‘Angostura’; and one from Sonora: ‘Sonora’) was evaluated in vivo in whiteleg shrimp P. vannamei infection assays. Differences were observed in shrimp mortality rates among the 3 isolates, of which Sonora was the most virulent. Subsequently, the complete genomes of the Sonora ...

Aquaculture and Fisheries, Nov 1, 2022

Melanization of shrimp gills is a general symptom in shrimp black bill diseases (sBG); therefore,... more Melanization of shrimp gills is a general symptom in shrimp black bill diseases (sBG); therefore, the cause is not immediately apparent. This study aimed to determine the primary and secondary agents associated with a black gill outbreak in the Delvar complex in the Bushehr Province of Iran. During July and August of 2020, 450 healthy and affected shrimps were collected based on brown to black gill color from three farms of the Bushehr Province in Iran. Characterization of pathogens was performed by gross signs, wet mounts, microbiological tests, histopathology methods, and sequencing of molecular markers. The mean weight and length of infected shrimps was significantly (P < 0.05) lower than the healthy shrimps. The histological examination of sBG indicated the adhesion of secondary lamellae, accumulation of hemocyte cells at the end of the secondary lamellae, and necrosis of epithelial cells with pyknosis and karyorrhexis. According to our results, Aspergillus sydowii as a primary agent correlates with sBG disease in Litopenaeus vannamei from Iran, and other biotic factors, including bacterial (Vibrio owensii and V. parahaemolyticus) probably are secondary agents associated with sBG disease.

Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 2007

Cell and Tissue Research, Sep 9, 2022

Branchial chambers constitute the main osmoregulatory site in almost all decapod crustaceans. How... more Branchial chambers constitute the main osmoregulatory site in almost all decapod crustaceans. However, few studies have been devoted to elucidate the cellular function of specific cells in every osmoregulatory structure of the branchial chambers. In decapod crustaceans, it is well-known that the osmoregulatory function is localized in specific structures that progressively specialize from early developmental stages while specific molecular mechanisms occur. In this study, we found that although the structures developed progressively during the larval and postlarval stages, before reaching juvenile or adult morphology, the osmoregulatory capabilities of Litopenaeus vannamei were gradually established only during the development of branchiostegites and epipodites, but not gills. The cellular structures of the branchial chambers observed during the larval phase do not present the typical ultrastructure of ionocytes, neither N a + /K +-ATPase expression, likely indicating that pleura, branchiostegites, or bud gills do not participate in osmoregulation. During early postlarval stages, the lack of N a + / K +-ATPase immunoreactivity of the ionocytes from the branchiostegites and epipodites suggests that they are immature ionocytes (ionocytes type I). It could be inferred from IIF and TEM results that epipodites and branchiostegites are involved in iono-osmoregulation from PL15, while gills and pleura do not participate in this function.

Archives of Oral Biology

OBJECTIVE The aim of this study was to investigate the effects of pre-pregnancy chronic exposure ... more OBJECTIVE The aim of this study was to investigate the effects of pre-pregnancy chronic exposure to Porphyromonas gingivalis LPS (Pg LPS) on the learning, memory, and seizure susceptibility of the offspring. DESIGN To achieve periodontitis, Pg LPS (5 μg/kg) was injected into the gingival of five female rats every 48 h for three weeks. Five control female rats received saline (0.9 %) and five female were kept intact. The concentrations of TNF-α and IL-6 were measured in the blood samples. One week after the final injection, females were mated with intact males. Following birth and weaning, two male and two female offspring were randomly selected from each mother, and new groups of male and female offspring were defined for behavioral assessments. Morris water maze was used to evaluate spatial memory, shuttle box was used to investigate avoidance memory and a pentylenetetrazole-induced seizure was used to evaluate seizure susceptibility in the offspring. RESULTS Spatial learning and avoidance memory significantly decreased in both male and female offspring of Pg LPS-exposed female rats, compared to the control offspring. Latency to reach seizure stages 1 and 2 significantly increased in the male offspring, but not the female offspring of Pg LPS-exposed female, compared to the control offspring. However, no significant difference was found in latency to reach stages 3-5. CONCLUSION Pre-pregnancy exposure to Pg LPS could affect some behavioral functions in both male and female offspring intergenerationally.

L'implication du tube digestif (TD) dans l'osmoregulation a ete etudiee durant le develop... more L'implication du tube digestif (TD) dans l'osmoregulation a ete etudiee durant le developpement du loup (bar) Dicentrarchus labrax. Differents niveaux d'integration (genes, proteines, cellule, physiologie, comportement) ont ete pris en compte, en liaison avec l'ecologie, dans une approche ecophysiologique. L'expression et la localisation des proteines impliquees dans la balance ionique et hydrique (ATPase Na+/K+, aquaporines AQP) ont ete suivies par histologie, microscopie electronique, immunofluorescence et biologie moleculaire. Les AQP1 et AQP3 ont ete sequencees et caracterisees. L'ontogenese du TD a ete decrite. Fonctionnel des l'eclosion, son implication dans l'osmoregulation s'accroit avec l'augmentation de la capacite osmoregulatrice a la transition metamorphique larve/juvenile. La capacite a absorber des ions precede celle de l'absorption de l'eau, en liaison respective avec l'expression de l'ATPase Na+/K+ et d'AQP1. La regulation de l'expression de ces proteines selon la salinite apparait au cours de la metamorphose. Le TD est fortement implique dans l'absorption d'eau compensant la deshydratation en eau de mer. L'exposition a l'eau douce provoque chez les juveniles des mortalites differentielles entre survivants (FWS) et ceux qui y meurent (FWU). Chez les FWU, la densite des tubules urinaires est plus faible dans le rein, ce qui limite la reabsorption ionique, et l'AQP1 est surexprimee dans le TD, entrainant probablement une augmentation de l'invasion d'eau via l'intestin. Ces deux phenomenes sont a l'origine de la faible osmolarite sanguine des FWU, cause de leur mort. Durant l'ontogenese du loup, les regulations combinees effectuees par le TD, le rein et les branchies repondent aux variations de salinite du milieu naturel, influant sur la tolerance a la salinite, et permettant le peuplement de divers milieux, de la mer aux lagunes et estuaires

Asian Fisheries Science, 2019

Aquaculture Research, 2018

Euryhaline fish, such as the Bullseye puffer Sphoeroides annulatus (Jenyns 1842), experience sudd... more Euryhaline fish, such as the Bullseye puffer Sphoeroides annulatus (Jenyns 1842), experience sudden salinity changes in their natural environment, which is more common than the exception, so they must adapt to survive and cope with extreme salt conditions. Therefore, Bullseye puffer juveniles were exposed to short-term stress (39 hr) by fluctuating salinity conditions (41,

Marine and Freshwater Behaviour and Physiology, 2015

We analysed the effects of salinity on survival, growth and the osmoregulatory capacity (OC) of p... more We analysed the effects of salinity on survival, growth and the osmoregulatory capacity (OC) of puffer fish larvae (Sphoeroides annulatus). In experiment 1, we compared the growth of larvae maintained at a constant temperature (28 ± 0.5 °C) under seven salinity levels (5, 12, 19, 26, 33, 35 and 40 psu) over a 28-day period. No significant differences were found for larval growth. However, salinity significantly affected survival. The larvae hyper-osmoregulated at low salinities and hypo-osmoregulated at high salinities. The isosmotic point ranged from 338.9 to 355.9 mOsm kg−1, depending on salinity. In experiment 2, survival increased with increasing salinity following direct exposure to the same seven salinity levels over a 72-h period. The isosmotic point ranged from 256.5 to 466.7 mOsm kg−1 depending on the developmental stage. This species hyper-regulated at 5–12 psu, hypo-regulated at 19–40 psu and demonstrated a high euryhalinity at 7 DAH with 95% survival.

Frontiers Research Topics, 2013

Este libro de texto invita tanto al lector aficionado como al especializado a conocer la lista de... more Este libro de texto invita tanto al lector aficionado como al especializado a conocer la lista de especies con potencial de cultivo en la zona del valle de Mexicali, Baja California, y explicita las razones y los métodos de selección de un limi-tado número de ellas a partir de una larga lista de candidatos disponibles a nivel nacional. Tiene también la intención de proveer a los tomadores de decisiones de una herramienta útil para elegir las zonas con mayor potencial para su cultivo, de modo que se pueda desarrollar esta actividad de manera nacional.