irene leal-berumen | Universidad Autonoma de Chihuahua (original) (raw)
Papers by irene leal-berumen
![Research paper thumbnail of [Comparative study of anterior cruciate ligament reconstruction between the One Band and U-Two techniques]](https://attachments.academia-assets.com/118214144/thumbnails/1.jpg)
](PubMed, Aug 9, 2020
Introduction: ACL injuries are found within the most common ligament injuries. This has led to th... more Introduction: ACL injuries are found within the most common ligament injuries. This has led to the development of different surgical techniques to restore knee stability. Material y methods: Clinical study, randomized, blind comparing ACL reconstruction techniques with One Band and «U-Two» using clinical scales and arthrometria with KT-1000 and Pivot Shift Meter. Results: Of 36 patients, 52.94% was drawn for the One Band technique and 47.06% for «U-Two». 11.76% of patients had a displacement greater than 11mm in the affected knee with KT-1000. 8.82% showed an equal anterior displacement on both knees. One patient had a major previous displacement after reconstruction surgery with the one band technique, but with improvement on clinical scales. Pivot Shift measurements found a difference in displacement greater than 4,000 m/s on the X axis following the One Band technique compared to the healthy knee calculating integrals, while with U-Two the difference was less than 600 m/s. On the Y axis a difference of 3,500 m/s was found between the healthy and the injured leg, which decreases to less than 2,000 m/s after reconstruction using U-Two technique. Conclusions: Differences have been found throughout the study in patient evolution, indicating that repair using the U-Dos technique has better results in recovering the anteroposterior and rotational stability of the affected limb.
American Journal of Human Biology, Feb 5, 2012
Objectives: To study the association between physical activity energy expenditure (PAEE) and card... more Objectives: To study the association between physical activity energy expenditure (PAEE) and cardio-respiratory fitness (CRF) with key metabolic traits and anthropometric measures in the Tarahumara of Mexico. Methods: A cross-sectional study was carried out in five rural communities in Chihuahua, México including 64 adult Tarahumara, mean (SD) age 40.7 (12.9) years. Using a combined accelerometer and heart rate sensor, PAEE was measured over three consecutive days and nights and a sub-maximal step test was carried out in order to (1) calibrate heart rate at the individual level and (2) to estimate CRF. Random blood glucose level and resting blood pressure (BP) were measured with standard anthropometrics. Results: Mean (SD) PAEE was 71.2 (30.3) kJ kg 21 day 21 and CRF was 36.6 (6.5) mlO 2 min 21 kg 21. Mean (SD) glucose was 127.9 (32.4) mg/dl, with 3.3% having diabetes. Mean (SD) systolic and diastolic BP was 122 (20.8) and 82 (14.8) mm Hg, respectively, with 28.1% having hypertension. Mean body mass index was 27.5 (4.2) kg m 22 , with 71.9% being overweight. Following adjustment for age and sex, weak inverse associations were observed between PAEE and systolic
PubMed, Jul 7, 2022
Introduction: The anterior cruciate ligament is an anatomical structure that tends to cause probl... more Introduction: The anterior cruciate ligament is an anatomical structure that tends to cause problems for specialists when formulating an accurate diagnosis. There are measurement tools that support the diagnosis such as the KT1000; however, it still has some limitations, since it only manages to measure a single axis of movement, while the "pivot-shift" maneuver manages to capture more information about the state of the ligament, as it allows to evaluate the three axes of movement of the joint. The "pivot-shift" maneuver lacks a standard execution, therefore it can produce disagreement between evaluators; this creates the need for a tool capable of standardizing the maneuver, while providing an interpretable measurement. Material and methods: Pivot-shift meter application, a mobile software capable of capturing information on the movements made when executing a pivot maneuver. Intraclass correlation coefficient to measure the intra- and inter-observer correlation of the information captured by the application. The data was obtained from 66 random participants, using the mobile phone's built-in gyroscopes. Results: With the 66 tests carried out, it was observed that the evaluators tend to achieve high reproducibility when executing the maneuvers within the time period established by the application, which reached an intraclass correlation coefficient above 90%. Conclusion: The intra- and inter-observer correlation is high enough to be able to perform a quantitative analysis of the pivot-shift maneuver, through a mobile phone.
Nutrients
The WHO identifies high BMI, high blood pressure, and high fasting plasma glucose as chronic dise... more The WHO identifies high BMI, high blood pressure, and high fasting plasma glucose as chronic disease risk factors, whereas physical fitness is identified as a protective behavioral factor. This study responds to the rising interest in assessing metabolic factors and physical activity within young populations of Mestizo, Tarahumara, and Mennonite from Chihuahua Mexico, due to its strong relationship with disease development and low well-being. A cross-sectional study was conducted with 201 teenagers from rural towns in Northern Mexico, and relationships between physical fitness and cardio-metabolic risk related to anthropometric, glycolipid, and vascular function factors were assessed. ANOVA-tested differences among ethnic groups using physical fitness as a grouping variable and measures of cardio-metabolic risks were used as dependent variables. A stepwise regression analysis allowed us to identify the best predictors for physical fitness. Clinical risk factors were analyzed by ethn...
Revista Chilena de Ortopedia y Traumatología, 2017
Introducción La lesión del ligamento cruzado anterior (LCA), es una patología frecuente, y el éxi... more Introducción La lesión del ligamento cruzado anterior (LCA), es una patología frecuente, y el éxito de la reconstrucción depende de diversos factores, tales como la técnica quirúrgica empleada, la edad del paciente, índice masa corporal, nivel de actividad y lesiones asociadas. El objetivo de este estudio es comparar los resultados funcionales a largo plazo de pacientes con lesiones condrales tratadas y sin lesiones condrales sometidos a la reconstrucción de LCA (RLCA), con autoinjerto semitendinoso-gracilis (ST-G). Materiales y Métodos Estudio analítico retrospectivo. Cuarenta y nueve pacientes (34 hombres y 15 mujeres), fueron sometidos a la RLCA con autoinjerto ST-G. Se compararon dos grupos. Grupo RLCA + LC: 24 pacientes con lesiones condrales ICRS grado III y IV tratadas en el mismo acto quirúrgico y grupo RLCA: 25 pacientes sin lesiones condrales concomitantes. Las edades promedio fueron de 32 y 28 años en cada grupo respectivamente. La funcionalidad de los pacientes a largo p...
![Research paper thumbnail of [Anterior cruciate ligament-plasty using the "U-dos" technique]](https://attachments.academia-assets.com/102967863/thumbnails/1.jpg)
](Acta ortopédica mexicana
The knee is a compound diarthrodial joint, vulnerable to serious injuries such as ligament injuri... more The knee is a compound diarthrodial joint, vulnerable to serious injuries such as ligament injuries of: medial collateral ligament, lateral collateral ligament, anterior cruciate ligament and posterior cruciate ligament, as cruciate ligaments limit rotation movement in the joint. The purpose of our study was to create a new technique to treat injuries of the anterior cruciate ligament, which is composed of two bundles--anteromedial and posterolateral--trying to achieve an anatomical reconstruction that allows for a normal biomechanical recovery. This technique reduces the use of fixation material and costs. The diagnosis of anterior cruciate ligament injuries was made with the pivot shift test. There are currently two repair methods for anterior cruciate ligament injuries: single bundle or double bundle repair; none of these techniques is considered as the gold standard, as their results are very similar. This paper describes a technique used for the treatment of anterior cruciate l...
Acta ortopedica mexicana, 2021
INTRODUCTION The diagnosis of ACL injury is established with the physical examination that includ... more INTRODUCTION The diagnosis of ACL injury is established with the physical examination that includes the Lachman maneuver and the pivot-shift test since it has the highest positive predictive value. Imaging tests such as MRI are still used for confirmation of the same; so the quantification of clinical maneuvers is sought. MATERIAL AND METHODS Prospective clinical study in which the maneuvers of pivot-shift and Lachman are quantified for later classification. RESULTS Of 36 patients, 64% found a range between 0 and 3 mm when subtracting the value of the affected knee from the value of the healthy knee. It was found that 28% of the patients recorded values of KT-1000 ranging between 4 and 6 mm of displacement. In 8% of the total patients, values of KT-1000 were found that exceeded 7 mm of displacement. The data obtained are normalized and classified into 3 degrees of injury: grade 1 with partial injury; grade 2 with complete anteromedial and partial injury in posterolateral bundle; gra...
Los ligamentos cruzados (LC) de la rodilla, anterior (A) y posterior (P), localizados dentro de l... more Los ligamentos cruzados (LC) de la rodilla, anterior (A) y posterior (P), localizados dentro de la articulacion, unen femur y tibia, dando estabilidad sobre la direccion anteroposterior de un hueso sobre otro. El LCA puede lesionarse mediante rotaciones provocadas en algun deporte que implique giro con el pie apoyado sobre el suelo. Las lesiones del LCA se determinan con maniobras como la de Lachman, cajon y pivote. Se utilizan acelerometros que permiten graficar la maniobra de pivote en pacientes que acuden a consulta ortopedica, utilizando la prueba de KT1000 como estandar de oro. El trabajo ha sido aprobado por el Comite de Etica del Hospital. Estudio descriptivo entre casos y controles. Resultados: 92 pacientes aceptaron participar mediante consentimiento informado, nueve casos resultaron con KT1000 positivo, de los negativos, seleccionamos nueve controles afines a edad y genero. Alteraciones de KT1000 fueron mayores en mujeres (78%) y en 67% de los casos la pierna alterada fue ...
RESUMEN. La rodilla es una articulación diartrósica compuesta y es vulnerable a lesiones graves c... more RESUMEN. La rodilla es una articulación diartrósica compuesta y es vulnerable a lesiones graves como son las ligamentarias: ligamento colateral medial, ligamento colateral lateral, ligamento cruzado anterior y ligamento cruzado posterior, ya que los ligamentos cruzados limitan el movimiento de rotación en la articulación. El objetivo de nuestro estudio fue crear una nueva técnica para las lesiones de ligamento cruzado anterior, la cual consta de dos haces: haz anteromedial y posterolateral, en busca de una reconstrucción anatómica que permita una recuperación biomecánica normal. Esta técnica reduce el uso de material de fi jación y los costos. El diagnóstico de las lesiones ligamento cruzado anterior se realizó con la prueba de Pivot Shift. Actualmente existen dos métodos de reparación en las lesiones de ligamento cruzado anterior, éstos son: reparando un solo haz o con doble haz; ninguna de estas técnicas es considerada como estándar de oro, ya que los resultados son muy similares....
Acta ortopedica mexicana, 2015
The anterior (A) and posterior (P) cruciate ligaments (CL) of the knee, located inside the joint,... more The anterior (A) and posterior (P) cruciate ligaments (CL) of the knee, located inside the joint, connect the femur and the tibia and thus provide stability in the anteroposterior axis of one bone over the other. The anterior cruciate ligament (ACL) may be injured as a result of rotation when practicing a sport involving turning with the foot on the ground. ACL injuries are diagnosed with maneuvers like the Lachman, drawer and pivot. Accelerometers were used to plot the pivot maneuver in patients seeing the orthopedist surgeon using the KT1000 test as gold standard. This case-control descriptive study was approved by the Hospital's Ethics Committee. Results: 92 patients accepted to participate through an informed consent; nine cases were KT1000 positive, and nine age- and gender-matched controls were selected among KT1000 negative patients. KT1000 alterations were greater among females (78%) and in 67% of cases the right leg was affected. Mean KT1000 results were 5.44 mm in case...
Tuberculosis, 2013
Tuberculosis (TB) and its co-morbid conditions have become a burden on global health economies. I... more Tuberculosis (TB) and its co-morbid conditions have become a burden on global health economies. It is well understood that susceptibility of the host to TB infection/disease is influenced by both genetic and environmental factors and their interactions. The aims of this pilot case-control study are to characterize the sociodemographic and environmental factors related to active TB disease (TB/case) and latent TB infection (LTBI/control) status, and to identify risk factors associated with progression from LTBI to TB. We recruited 75 cases with TB (mean age=46.3y; females=41%) and 75 controls with LTBI (mean age=39.0y; females=37%), from the Mestizo population of Cuidad Juárez, Mexico. In addition to the determination of case/control status, information on environmental variables was collected (e.g., socioeconomic status, smoking, alcohol consumption, substance abuse, nutritional status, household demographics, medical histories and presence of type 2 diabetes [T2DM]). The data were analyzed to identify the environmental correlates of TB and LTBI using univariate and multivariate statistical approaches. Following multivariate logistic regression analysis, TB was associated with poor nutrition, T2DM, family history of TB, and non-Chihuahua state of birth. These preliminary findings have relevance to TB control at the Mexico-United States border, and contribute to our future genetic study of TB in Mexicans.
Journal of immunology (Baltimore, Md. : 1950), 1996
Mast cells have been reported to secrete a wide range of immunoregulatory cytokines following IgE... more Mast cells have been reported to secrete a wide range of immunoregulatory cytokines following IgE-mediated activation and to play an important role in allergic inflammation. We have previously demonstrated that mast cells can also produce certain cytokines following activation with bacterial LPS or prostanoids without preformed mediator release. IL-12 is a potent inducer of IFN-gamma production by T cells and NK cells, and is thought to play a critical role in determining the nature of the local immune response to infection. We here report that highly purified peritoneal mast cells from Brown Norway rats will produce IFN-gamma in response to IL-12 without significant histamine release. IFN-gamma protein was detected by ELISA in supernatants of mast cells cultured with 2 U/ml recombinant mouse IL-12 for between 6 and 24 h. The production of IFN-gamma was dependent on the dose of IL-12 and was significantly inhibited by concurrent treatment with IL-10 or PGE2. Supernatants from IL-12-...
Journal of immunology (Baltimore, Md. : 1950), 1996
Mast cells have been traditionally associated with an acute allergic response. However, their rol... more Mast cells have been traditionally associated with an acute allergic response. However, their role in regulating chronic inflammatory processes must also be considered in view of evidence that mast cells synthesize and release a number of cytokines. In this study, we have examined the effect of cholera toxin (CT) on peritoneal mast cell IL-6 and TNF-alpha production. Highly purified, freshly isolated, rat peritoneal mast cells from Brown Norway rats were cultured in the presence of CT or its B subunit (CTB) alone or in combination with anti-IgE or bacterial LPS. Histamine release was measured after 10 min; IL-16 and TNF-alpha production was assessed in supernatants after 18 h. We found that CT or CTB alone did not affect histamine release; however, mast cell IL-6 production was significantly enhanced by CT but not by CTB. In contrast, constitutive production of TNF-alpha was inhibited by CT. The effects of CT were similar to our previous observations of the actions of prostaglandin ...
Journal of immunology (Baltimore, Md. : 1950), 1995
Mast cells are traditionally associated with an acute response involving the short-term release o... more Mast cells are traditionally associated with an acute response involving the short-term release of mediators such as histamine. We have shown previously that mast cells can produce IL-6 without prior histamine release. In this study we examined the hypothesis that mast cell IL-6 production can be selectively regulated by PGs. Highly purified rat peritoneal mast cells were cultured in the presence of PGE1, PGE2, or PGD2 alone or in combination with anti-IgE or bacterial LPS. Histamine release was assessed after 10 min; IL-6 and TNF-alpha production was measured in supernatants after 18 h. Mast cell IL-6 production was induced by PGE1 and PGE2 to a similar level to that observed in anti-IgE-activated cells. In contrast, constitutive production of TNF-alpha was inhibited by PGE1 and PGE2, but not by PGD2. PGE2 had a synergistic effect, inducing IL-6 in the presence of LPS, whereas an additive effect was observed in the presence of anti-IgE. None of the prostanoids alone induced signifi...
Journal of Clinical Investigation, 1996
Mast cells have been implicated in a number of diseases involving chronic inflammation including ... more Mast cells have been implicated in a number of diseases involving chronic inflammation including asthma, rheumatoid arthritis, and inflammatory bowel diseases. They are a potent source of several cytokines, including IL-6 and TNF-␣. Freshly isolated rat peritoneal mast cells will produce IL-6 in response to anti-IgE, LPS, PGE 1 , or PGE 2 ; however, the mechanisms by which such cytokine production is regulated are poorly understood. IL-10 is recognized as an important immunoregulatory cytokine with effects on T cell development and the production of inflammatory cytokines. IL-10 has previously been described to enhance mast cell development in the context of IL-3 and IL-4. In the current study, we have examined the ability of IL-10 to modulate rat peritoneal mast cell IL-6 and TNF-␣ production in response to a variety of stimuli. We have observed that recombinant murine IL-10 can inhibit the production of both IL-6 and TNF-␣ by mast cells without altering the degree of histamine release in response to anti-IgE. Concentrations of IL-10 as low as 0.2 ng/ml were sufficient to inhibit IL-6 production by LPS-or anti-IgE-activated cells significantly. IL-10 also inhibited PGE 1-and PGE 2-induced IL-6 production. The relative potency of IL-10 as an inhibitor of mast cell IL-6 production was highly dependent upon the stimulus used, with a 10-fold difference in the IC 50 for LPS-or anti-IgEactivated cells (0.21 ng/ml) and cells activated with a combination of LPS and PGE 2 (2.29 ng/ml). This suggests that prostanoids may limit the ability of IL-10 to modulate mast cell IL-6 production in the context of inflammation. These data have important implications for the regulation of mast cell IL-6 in inflammatory diseases involving prostanoid production and the effects of treatment with cyclooxygenase inhibitors. Our results also demonstrate a dual role for IL-10 on mast cells as a growth factor and inhibitor of cytokine production.
En el año de 2019 en la provincia de Wuhan, China, se reportó un brote de neumonías atípicas, con... more En el año de 2019 en la provincia de Wuhan, China, se reportó un brote de neumonías atípicas, con síntomas similares a los del Síndrome Respiratorio Agudo Severo (SARS), brote reportado en el 2003, y al Síndrome Respiratorio del Oriente Medio (MERS), brote reportado en 2012 en Arabia Saudita. Esta enfermedad emergente fue causada por el nuevo coronavirus “Síndrome Respiratorio Agudo Severo Coronavirus 2” (SARS-CoV-2) o también denominada COVID-19 del inglés Coronavirus desease 2019, caracterizado por presentar severos problemas respiratorios y diversas manifestaciones extrapulmonares. Al momento se ha reportado una elevada tasa de mortalidad en individuos del sexo masculino, en adultos mayores de 60 años, y en personas con comorbilidades como diabetes obesidad, hipertensión y problemas cardiacos. La diabetes es una enfermedad caracterizada por resistencia a insulina, pérdida de función de células β en el páncreas, y desbalance del sistema inmune por el estado de inflamación crónica...
The journal of knee surgery, 2002
Synovial tissue is a highly specialized tissue that keeps the articular joint well lubricated, an... more Synovial tissue is a highly specialized tissue that keeps the articular joint well lubricated, and at the same time provides nutrients to the articular surface. A joint needs a small amount of synovial fluid to work. Although the tissue structure is simple, its function is highly sophisticated. Because synovial tissue is the target of most autoimmune diseases, joints need to be understood in their entirety to create new treatments for such immunological disorders.
Biology
We previously reported preliminary characterization of adipose tissue (AT) dysfunction through th... more We previously reported preliminary characterization of adipose tissue (AT) dysfunction through the adiponectin/leptin ratio (ALR) and fasting/postprandial (F/P) gene expression in subcutaneous (SQ) adipose tissue (AT) biopsies obtained from participants in the GEMM study, a precision medicine research project. Here we present integrative data replication of previous findings from an increased number of GEMM symptom-free (SF) adults (N = 124) to improve characterization of early biomarkers for cardiovascular (CV)/immunometabolic risk in SF adults with AT dysfunction. We achieved this goal by taking advantage of the rich set of GEMM F/P 5 h time course data and three tissue samples collected at the same time and frequency on each adult participant (F/P blood, biopsies of SQAT and skeletal muscle (SKM)). We classified them with the presence/absence of AT dysfunction: low (<1) or high (>1) ALR. We also examined the presence of metabolically healthy (MH)/unhealthy (MUH) individuals...
ABSTRACT The mast cell has been implicated as an initiating cell in the immediate responses to al... more ABSTRACT The mast cell has been implicated as an initiating cell in the immediate responses to allergen challenge where preformed mediators such as histamine play an important role. However, the role of mast cells is less well understood in severe allergic disorders such as asthma where chronic inflammatory changes are present. Results presented in this thesis demonstrate that freshly isolated and highly purified rat peritoneal mast cells can release IL-6 without any necessity for histamine release. These observations were determined with the use of bacterial products such as LPS and CT which significantly enhanced IL-6 production in our system.^ Prostanoids of the E family (PGE$\sb1$ and PGE$\sb2)$ were also used as stimulating agents which may also participate in inflammation. We observed that these prostaglandins selectively enhanced IL-6 production while TNF-$\alpha$ synthesis significantly inhibited CT was observed to have very similar effects to PGE$\sb1$ on mast cells. These findings illustrate the potential role that mast cells may have during chronic inflammation and infectious disease. ^
THE Coatings, 2021
Air plasma spray technique (APS) is widely used in the biomedical industry for the development of... more Air plasma spray technique (APS) is widely used in the biomedical industry for the development of HA-based biocoatings. The present study focuses on the influence of powder homogenization treatment by high-energy ball milling (HEBM) in developing a novel hydroxyapatite-barium titanate (HA/BT) composite coating deposited by APS; in order to compare the impact of the milling process, powders were homogenized by mechanical stirring homogenization (MSH) too. For the two-homogenization process, three weight percent ratios were studied; 10%, 30%, and 50% w/w of BT in the HA matrix. The phase and crystallite size were analyzed by X-ray diffraction patterns (XRD); the BT-phase distribution in the coating was analyzed by backscattered electron image (BSE) with a scanning electron microscope (SEM); the energy-dispersive X-ray spectroscopy (EDS) analysis was used to determinate the Ca/P molar ratio of the coatings, the degree of adhesion (bonding strength) of coatings was determinate by pull-o...
PubMed, Aug 9, 2020
Introduction: ACL injuries are found within the most common ligament injuries. This has led to th... more Introduction: ACL injuries are found within the most common ligament injuries. This has led to the development of different surgical techniques to restore knee stability. Material y methods: Clinical study, randomized, blind comparing ACL reconstruction techniques with One Band and «U-Two» using clinical scales and arthrometria with KT-1000 and Pivot Shift Meter. Results: Of 36 patients, 52.94% was drawn for the One Band technique and 47.06% for «U-Two». 11.76% of patients had a displacement greater than 11mm in the affected knee with KT-1000. 8.82% showed an equal anterior displacement on both knees. One patient had a major previous displacement after reconstruction surgery with the one band technique, but with improvement on clinical scales. Pivot Shift measurements found a difference in displacement greater than 4,000 m/s on the X axis following the One Band technique compared to the healthy knee calculating integrals, while with U-Two the difference was less than 600 m/s. On the Y axis a difference of 3,500 m/s was found between the healthy and the injured leg, which decreases to less than 2,000 m/s after reconstruction using U-Two technique. Conclusions: Differences have been found throughout the study in patient evolution, indicating that repair using the U-Dos technique has better results in recovering the anteroposterior and rotational stability of the affected limb.
American Journal of Human Biology, Feb 5, 2012
Objectives: To study the association between physical activity energy expenditure (PAEE) and card... more Objectives: To study the association between physical activity energy expenditure (PAEE) and cardio-respiratory fitness (CRF) with key metabolic traits and anthropometric measures in the Tarahumara of Mexico. Methods: A cross-sectional study was carried out in five rural communities in Chihuahua, México including 64 adult Tarahumara, mean (SD) age 40.7 (12.9) years. Using a combined accelerometer and heart rate sensor, PAEE was measured over three consecutive days and nights and a sub-maximal step test was carried out in order to (1) calibrate heart rate at the individual level and (2) to estimate CRF. Random blood glucose level and resting blood pressure (BP) were measured with standard anthropometrics. Results: Mean (SD) PAEE was 71.2 (30.3) kJ kg 21 day 21 and CRF was 36.6 (6.5) mlO 2 min 21 kg 21. Mean (SD) glucose was 127.9 (32.4) mg/dl, with 3.3% having diabetes. Mean (SD) systolic and diastolic BP was 122 (20.8) and 82 (14.8) mm Hg, respectively, with 28.1% having hypertension. Mean body mass index was 27.5 (4.2) kg m 22 , with 71.9% being overweight. Following adjustment for age and sex, weak inverse associations were observed between PAEE and systolic
PubMed, Jul 7, 2022
Introduction: The anterior cruciate ligament is an anatomical structure that tends to cause probl... more Introduction: The anterior cruciate ligament is an anatomical structure that tends to cause problems for specialists when formulating an accurate diagnosis. There are measurement tools that support the diagnosis such as the KT1000; however, it still has some limitations, since it only manages to measure a single axis of movement, while the "pivot-shift" maneuver manages to capture more information about the state of the ligament, as it allows to evaluate the three axes of movement of the joint. The "pivot-shift" maneuver lacks a standard execution, therefore it can produce disagreement between evaluators; this creates the need for a tool capable of standardizing the maneuver, while providing an interpretable measurement. Material and methods: Pivot-shift meter application, a mobile software capable of capturing information on the movements made when executing a pivot maneuver. Intraclass correlation coefficient to measure the intra- and inter-observer correlation of the information captured by the application. The data was obtained from 66 random participants, using the mobile phone's built-in gyroscopes. Results: With the 66 tests carried out, it was observed that the evaluators tend to achieve high reproducibility when executing the maneuvers within the time period established by the application, which reached an intraclass correlation coefficient above 90%. Conclusion: The intra- and inter-observer correlation is high enough to be able to perform a quantitative analysis of the pivot-shift maneuver, through a mobile phone.
Nutrients
The WHO identifies high BMI, high blood pressure, and high fasting plasma glucose as chronic dise... more The WHO identifies high BMI, high blood pressure, and high fasting plasma glucose as chronic disease risk factors, whereas physical fitness is identified as a protective behavioral factor. This study responds to the rising interest in assessing metabolic factors and physical activity within young populations of Mestizo, Tarahumara, and Mennonite from Chihuahua Mexico, due to its strong relationship with disease development and low well-being. A cross-sectional study was conducted with 201 teenagers from rural towns in Northern Mexico, and relationships between physical fitness and cardio-metabolic risk related to anthropometric, glycolipid, and vascular function factors were assessed. ANOVA-tested differences among ethnic groups using physical fitness as a grouping variable and measures of cardio-metabolic risks were used as dependent variables. A stepwise regression analysis allowed us to identify the best predictors for physical fitness. Clinical risk factors were analyzed by ethn...
Revista Chilena de Ortopedia y Traumatología, 2017
Introducción La lesión del ligamento cruzado anterior (LCA), es una patología frecuente, y el éxi... more Introducción La lesión del ligamento cruzado anterior (LCA), es una patología frecuente, y el éxito de la reconstrucción depende de diversos factores, tales como la técnica quirúrgica empleada, la edad del paciente, índice masa corporal, nivel de actividad y lesiones asociadas. El objetivo de este estudio es comparar los resultados funcionales a largo plazo de pacientes con lesiones condrales tratadas y sin lesiones condrales sometidos a la reconstrucción de LCA (RLCA), con autoinjerto semitendinoso-gracilis (ST-G). Materiales y Métodos Estudio analítico retrospectivo. Cuarenta y nueve pacientes (34 hombres y 15 mujeres), fueron sometidos a la RLCA con autoinjerto ST-G. Se compararon dos grupos. Grupo RLCA + LC: 24 pacientes con lesiones condrales ICRS grado III y IV tratadas en el mismo acto quirúrgico y grupo RLCA: 25 pacientes sin lesiones condrales concomitantes. Las edades promedio fueron de 32 y 28 años en cada grupo respectivamente. La funcionalidad de los pacientes a largo p...
Acta ortopédica mexicana
The knee is a compound diarthrodial joint, vulnerable to serious injuries such as ligament injuri... more The knee is a compound diarthrodial joint, vulnerable to serious injuries such as ligament injuries of: medial collateral ligament, lateral collateral ligament, anterior cruciate ligament and posterior cruciate ligament, as cruciate ligaments limit rotation movement in the joint. The purpose of our study was to create a new technique to treat injuries of the anterior cruciate ligament, which is composed of two bundles--anteromedial and posterolateral--trying to achieve an anatomical reconstruction that allows for a normal biomechanical recovery. This technique reduces the use of fixation material and costs. The diagnosis of anterior cruciate ligament injuries was made with the pivot shift test. There are currently two repair methods for anterior cruciate ligament injuries: single bundle or double bundle repair; none of these techniques is considered as the gold standard, as their results are very similar. This paper describes a technique used for the treatment of anterior cruciate l...
Acta ortopedica mexicana, 2021
INTRODUCTION The diagnosis of ACL injury is established with the physical examination that includ... more INTRODUCTION The diagnosis of ACL injury is established with the physical examination that includes the Lachman maneuver and the pivot-shift test since it has the highest positive predictive value. Imaging tests such as MRI are still used for confirmation of the same; so the quantification of clinical maneuvers is sought. MATERIAL AND METHODS Prospective clinical study in which the maneuvers of pivot-shift and Lachman are quantified for later classification. RESULTS Of 36 patients, 64% found a range between 0 and 3 mm when subtracting the value of the affected knee from the value of the healthy knee. It was found that 28% of the patients recorded values of KT-1000 ranging between 4 and 6 mm of displacement. In 8% of the total patients, values of KT-1000 were found that exceeded 7 mm of displacement. The data obtained are normalized and classified into 3 degrees of injury: grade 1 with partial injury; grade 2 with complete anteromedial and partial injury in posterolateral bundle; gra...
Los ligamentos cruzados (LC) de la rodilla, anterior (A) y posterior (P), localizados dentro de l... more Los ligamentos cruzados (LC) de la rodilla, anterior (A) y posterior (P), localizados dentro de la articulacion, unen femur y tibia, dando estabilidad sobre la direccion anteroposterior de un hueso sobre otro. El LCA puede lesionarse mediante rotaciones provocadas en algun deporte que implique giro con el pie apoyado sobre el suelo. Las lesiones del LCA se determinan con maniobras como la de Lachman, cajon y pivote. Se utilizan acelerometros que permiten graficar la maniobra de pivote en pacientes que acuden a consulta ortopedica, utilizando la prueba de KT1000 como estandar de oro. El trabajo ha sido aprobado por el Comite de Etica del Hospital. Estudio descriptivo entre casos y controles. Resultados: 92 pacientes aceptaron participar mediante consentimiento informado, nueve casos resultaron con KT1000 positivo, de los negativos, seleccionamos nueve controles afines a edad y genero. Alteraciones de KT1000 fueron mayores en mujeres (78%) y en 67% de los casos la pierna alterada fue ...
RESUMEN. La rodilla es una articulación diartrósica compuesta y es vulnerable a lesiones graves c... more RESUMEN. La rodilla es una articulación diartrósica compuesta y es vulnerable a lesiones graves como son las ligamentarias: ligamento colateral medial, ligamento colateral lateral, ligamento cruzado anterior y ligamento cruzado posterior, ya que los ligamentos cruzados limitan el movimiento de rotación en la articulación. El objetivo de nuestro estudio fue crear una nueva técnica para las lesiones de ligamento cruzado anterior, la cual consta de dos haces: haz anteromedial y posterolateral, en busca de una reconstrucción anatómica que permita una recuperación biomecánica normal. Esta técnica reduce el uso de material de fi jación y los costos. El diagnóstico de las lesiones ligamento cruzado anterior se realizó con la prueba de Pivot Shift. Actualmente existen dos métodos de reparación en las lesiones de ligamento cruzado anterior, éstos son: reparando un solo haz o con doble haz; ninguna de estas técnicas es considerada como estándar de oro, ya que los resultados son muy similares....
Acta ortopedica mexicana, 2015
The anterior (A) and posterior (P) cruciate ligaments (CL) of the knee, located inside the joint,... more The anterior (A) and posterior (P) cruciate ligaments (CL) of the knee, located inside the joint, connect the femur and the tibia and thus provide stability in the anteroposterior axis of one bone over the other. The anterior cruciate ligament (ACL) may be injured as a result of rotation when practicing a sport involving turning with the foot on the ground. ACL injuries are diagnosed with maneuvers like the Lachman, drawer and pivot. Accelerometers were used to plot the pivot maneuver in patients seeing the orthopedist surgeon using the KT1000 test as gold standard. This case-control descriptive study was approved by the Hospital's Ethics Committee. Results: 92 patients accepted to participate through an informed consent; nine cases were KT1000 positive, and nine age- and gender-matched controls were selected among KT1000 negative patients. KT1000 alterations were greater among females (78%) and in 67% of cases the right leg was affected. Mean KT1000 results were 5.44 mm in case...
Tuberculosis, 2013
Tuberculosis (TB) and its co-morbid conditions have become a burden on global health economies. I... more Tuberculosis (TB) and its co-morbid conditions have become a burden on global health economies. It is well understood that susceptibility of the host to TB infection/disease is influenced by both genetic and environmental factors and their interactions. The aims of this pilot case-control study are to characterize the sociodemographic and environmental factors related to active TB disease (TB/case) and latent TB infection (LTBI/control) status, and to identify risk factors associated with progression from LTBI to TB. We recruited 75 cases with TB (mean age=46.3y; females=41%) and 75 controls with LTBI (mean age=39.0y; females=37%), from the Mestizo population of Cuidad Juárez, Mexico. In addition to the determination of case/control status, information on environmental variables was collected (e.g., socioeconomic status, smoking, alcohol consumption, substance abuse, nutritional status, household demographics, medical histories and presence of type 2 diabetes [T2DM]). The data were analyzed to identify the environmental correlates of TB and LTBI using univariate and multivariate statistical approaches. Following multivariate logistic regression analysis, TB was associated with poor nutrition, T2DM, family history of TB, and non-Chihuahua state of birth. These preliminary findings have relevance to TB control at the Mexico-United States border, and contribute to our future genetic study of TB in Mexicans.
Journal of immunology (Baltimore, Md. : 1950), 1996
Mast cells have been reported to secrete a wide range of immunoregulatory cytokines following IgE... more Mast cells have been reported to secrete a wide range of immunoregulatory cytokines following IgE-mediated activation and to play an important role in allergic inflammation. We have previously demonstrated that mast cells can also produce certain cytokines following activation with bacterial LPS or prostanoids without preformed mediator release. IL-12 is a potent inducer of IFN-gamma production by T cells and NK cells, and is thought to play a critical role in determining the nature of the local immune response to infection. We here report that highly purified peritoneal mast cells from Brown Norway rats will produce IFN-gamma in response to IL-12 without significant histamine release. IFN-gamma protein was detected by ELISA in supernatants of mast cells cultured with 2 U/ml recombinant mouse IL-12 for between 6 and 24 h. The production of IFN-gamma was dependent on the dose of IL-12 and was significantly inhibited by concurrent treatment with IL-10 or PGE2. Supernatants from IL-12-...
Journal of immunology (Baltimore, Md. : 1950), 1996
Mast cells have been traditionally associated with an acute allergic response. However, their rol... more Mast cells have been traditionally associated with an acute allergic response. However, their role in regulating chronic inflammatory processes must also be considered in view of evidence that mast cells synthesize and release a number of cytokines. In this study, we have examined the effect of cholera toxin (CT) on peritoneal mast cell IL-6 and TNF-alpha production. Highly purified, freshly isolated, rat peritoneal mast cells from Brown Norway rats were cultured in the presence of CT or its B subunit (CTB) alone or in combination with anti-IgE or bacterial LPS. Histamine release was measured after 10 min; IL-16 and TNF-alpha production was assessed in supernatants after 18 h. We found that CT or CTB alone did not affect histamine release; however, mast cell IL-6 production was significantly enhanced by CT but not by CTB. In contrast, constitutive production of TNF-alpha was inhibited by CT. The effects of CT were similar to our previous observations of the actions of prostaglandin ...
Journal of immunology (Baltimore, Md. : 1950), 1995
Mast cells are traditionally associated with an acute response involving the short-term release o... more Mast cells are traditionally associated with an acute response involving the short-term release of mediators such as histamine. We have shown previously that mast cells can produce IL-6 without prior histamine release. In this study we examined the hypothesis that mast cell IL-6 production can be selectively regulated by PGs. Highly purified rat peritoneal mast cells were cultured in the presence of PGE1, PGE2, or PGD2 alone or in combination with anti-IgE or bacterial LPS. Histamine release was assessed after 10 min; IL-6 and TNF-alpha production was measured in supernatants after 18 h. Mast cell IL-6 production was induced by PGE1 and PGE2 to a similar level to that observed in anti-IgE-activated cells. In contrast, constitutive production of TNF-alpha was inhibited by PGE1 and PGE2, but not by PGD2. PGE2 had a synergistic effect, inducing IL-6 in the presence of LPS, whereas an additive effect was observed in the presence of anti-IgE. None of the prostanoids alone induced signifi...
Journal of Clinical Investigation, 1996
Mast cells have been implicated in a number of diseases involving chronic inflammation including ... more Mast cells have been implicated in a number of diseases involving chronic inflammation including asthma, rheumatoid arthritis, and inflammatory bowel diseases. They are a potent source of several cytokines, including IL-6 and TNF-␣. Freshly isolated rat peritoneal mast cells will produce IL-6 in response to anti-IgE, LPS, PGE 1 , or PGE 2 ; however, the mechanisms by which such cytokine production is regulated are poorly understood. IL-10 is recognized as an important immunoregulatory cytokine with effects on T cell development and the production of inflammatory cytokines. IL-10 has previously been described to enhance mast cell development in the context of IL-3 and IL-4. In the current study, we have examined the ability of IL-10 to modulate rat peritoneal mast cell IL-6 and TNF-␣ production in response to a variety of stimuli. We have observed that recombinant murine IL-10 can inhibit the production of both IL-6 and TNF-␣ by mast cells without altering the degree of histamine release in response to anti-IgE. Concentrations of IL-10 as low as 0.2 ng/ml were sufficient to inhibit IL-6 production by LPS-or anti-IgE-activated cells significantly. IL-10 also inhibited PGE 1-and PGE 2-induced IL-6 production. The relative potency of IL-10 as an inhibitor of mast cell IL-6 production was highly dependent upon the stimulus used, with a 10-fold difference in the IC 50 for LPS-or anti-IgEactivated cells (0.21 ng/ml) and cells activated with a combination of LPS and PGE 2 (2.29 ng/ml). This suggests that prostanoids may limit the ability of IL-10 to modulate mast cell IL-6 production in the context of inflammation. These data have important implications for the regulation of mast cell IL-6 in inflammatory diseases involving prostanoid production and the effects of treatment with cyclooxygenase inhibitors. Our results also demonstrate a dual role for IL-10 on mast cells as a growth factor and inhibitor of cytokine production.
En el año de 2019 en la provincia de Wuhan, China, se reportó un brote de neumonías atípicas, con... more En el año de 2019 en la provincia de Wuhan, China, se reportó un brote de neumonías atípicas, con síntomas similares a los del Síndrome Respiratorio Agudo Severo (SARS), brote reportado en el 2003, y al Síndrome Respiratorio del Oriente Medio (MERS), brote reportado en 2012 en Arabia Saudita. Esta enfermedad emergente fue causada por el nuevo coronavirus “Síndrome Respiratorio Agudo Severo Coronavirus 2” (SARS-CoV-2) o también denominada COVID-19 del inglés Coronavirus desease 2019, caracterizado por presentar severos problemas respiratorios y diversas manifestaciones extrapulmonares. Al momento se ha reportado una elevada tasa de mortalidad en individuos del sexo masculino, en adultos mayores de 60 años, y en personas con comorbilidades como diabetes obesidad, hipertensión y problemas cardiacos. La diabetes es una enfermedad caracterizada por resistencia a insulina, pérdida de función de células β en el páncreas, y desbalance del sistema inmune por el estado de inflamación crónica...
The journal of knee surgery, 2002
Synovial tissue is a highly specialized tissue that keeps the articular joint well lubricated, an... more Synovial tissue is a highly specialized tissue that keeps the articular joint well lubricated, and at the same time provides nutrients to the articular surface. A joint needs a small amount of synovial fluid to work. Although the tissue structure is simple, its function is highly sophisticated. Because synovial tissue is the target of most autoimmune diseases, joints need to be understood in their entirety to create new treatments for such immunological disorders.
Biology
We previously reported preliminary characterization of adipose tissue (AT) dysfunction through th... more We previously reported preliminary characterization of adipose tissue (AT) dysfunction through the adiponectin/leptin ratio (ALR) and fasting/postprandial (F/P) gene expression in subcutaneous (SQ) adipose tissue (AT) biopsies obtained from participants in the GEMM study, a precision medicine research project. Here we present integrative data replication of previous findings from an increased number of GEMM symptom-free (SF) adults (N = 124) to improve characterization of early biomarkers for cardiovascular (CV)/immunometabolic risk in SF adults with AT dysfunction. We achieved this goal by taking advantage of the rich set of GEMM F/P 5 h time course data and three tissue samples collected at the same time and frequency on each adult participant (F/P blood, biopsies of SQAT and skeletal muscle (SKM)). We classified them with the presence/absence of AT dysfunction: low (<1) or high (>1) ALR. We also examined the presence of metabolically healthy (MH)/unhealthy (MUH) individuals...
ABSTRACT The mast cell has been implicated as an initiating cell in the immediate responses to al... more ABSTRACT The mast cell has been implicated as an initiating cell in the immediate responses to allergen challenge where preformed mediators such as histamine play an important role. However, the role of mast cells is less well understood in severe allergic disorders such as asthma where chronic inflammatory changes are present. Results presented in this thesis demonstrate that freshly isolated and highly purified rat peritoneal mast cells can release IL-6 without any necessity for histamine release. These observations were determined with the use of bacterial products such as LPS and CT which significantly enhanced IL-6 production in our system.^ Prostanoids of the E family (PGE$\sb1$ and PGE$\sb2)$ were also used as stimulating agents which may also participate in inflammation. We observed that these prostaglandins selectively enhanced IL-6 production while TNF-$\alpha$ synthesis significantly inhibited CT was observed to have very similar effects to PGE$\sb1$ on mast cells. These findings illustrate the potential role that mast cells may have during chronic inflammation and infectious disease. ^
THE Coatings, 2021
Air plasma spray technique (APS) is widely used in the biomedical industry for the development of... more Air plasma spray technique (APS) is widely used in the biomedical industry for the development of HA-based biocoatings. The present study focuses on the influence of powder homogenization treatment by high-energy ball milling (HEBM) in developing a novel hydroxyapatite-barium titanate (HA/BT) composite coating deposited by APS; in order to compare the impact of the milling process, powders were homogenized by mechanical stirring homogenization (MSH) too. For the two-homogenization process, three weight percent ratios were studied; 10%, 30%, and 50% w/w of BT in the HA matrix. The phase and crystallite size were analyzed by X-ray diffraction patterns (XRD); the BT-phase distribution in the coating was analyzed by backscattered electron image (BSE) with a scanning electron microscope (SEM); the energy-dispersive X-ray spectroscopy (EDS) analysis was used to determinate the Ca/P molar ratio of the coatings, the degree of adhesion (bonding strength) of coatings was determinate by pull-o...