Gaber Ramadan | UAE University (original) (raw)

Papers by Gaber Ramadan

Research paper thumbnail of Synthesis of New Chromene Derivatives Targeting Triple-Negative Breast Cancer Cells

Cancers

Breast cancer continues to be the leading cause of cancer-related deaths among women worldwide. T... more Breast cancer continues to be the leading cause of cancer-related deaths among women worldwide. The most aggressive type of breast cancer is triple-negative breast cancer (TNBC). Indeed, not only does TNBC not respond well to several chemotherapeutic agents, but it also frequently develops resistance to various anti-cancer drugs, including taxane mitotic inhibitors. This necessitates the search for newer, more efficacious drugs. In this study, we synthesized two novel chromene derivatives (C1 and C2) and tested their efficacy against a battery of luminal type A and TNBC cell lines. Our results show that C1 and C2 significantly and specifically inhibited TNBC cell viability but had no effect on the luminal A cell type. In addition, these novel compounds induced mitotic arrest, cell multinucleation leading to senescence, and apoptotic cell death through the activation of the extrinsic pathway. We also showed that the underlying mechanisms for these actions of C1 and C2 involved inhibi...

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Research paper thumbnail of Hypothetic model illustrating the differential effect of carnosol in MDA-MB-231 breast cancer cells

<p>Hypothetic model illustrating the differential effect of carnosol in MDA-MB-231 breast c... more <p>Hypothetic model illustrating the differential effect of carnosol in MDA-MB-231 breast cancer cells.</p

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Research paper thumbnail of Induction of caspases-mediated apoptosis by carnosol in the MDA-MB-231 cells

<p>(<b>A</b>) Carnosol induced apoptosis in the MDA-MB-231 cells. Annexin V bin... more <p>(<b>A</b>) Carnosol induced apoptosis in the MDA-MB-231 cells. Annexin V binding was carried out using Annexin V & Dead Cell kit (Millipore). Cells were treated with DMSO or various concentration of carnosol for 24 h. Detached and adherent cells were collected and stained with Annexin V and 7-AAD and then the events for early and late apoptotic cells were counted with the Muse Cell Analyzer as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109630#s2&quot; target="_blank">Materials and Methods</a>. (<b>B</b>) Western blot analysis of caspase 3, -9, and -8 activation, PARP cleavage and Bcl1 and Bax expression in MDA-MB-231 cells were treated with increasing concentrations of carnosol (25, 50 and 100 µM) for 24 h. (<b>C</b>) Carnosol induces the depolarization of mitochondrial membrane. Mitochondrial membrane potential (MMP) was assessed with the Muse Cell Analyzer using the Muse MitoPotential kit as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109630#s2&quot; target="_blank">Materials and Methods</a>. Data represent the mean ±SEM of at least 3 independent experiments. Student's t test was performed to determine the significance (*<i>p</i><0.05, **<i>p</i><0.01 and ***<i>p</i><0.005).</p

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Research paper thumbnail of The Multifarious Endophytic Actinobacterial Isolate, Streptomyces tubercidicus UAE1, Combined With the Seaweed Biostimulant Further Promotes Growth of Avicennia marina

Frontiers in Marine Science

Mangrove (Avicennia marina) is a “green lung” tree growing along the Arabian Gulf coastline in th... more Mangrove (Avicennia marina) is a “green lung” tree growing along the Arabian Gulf coastline in the United Arab Emirates. Here, we aimed to determine the impact of the application of a commercial seaweed extract (SWE) biostimulant and endophytic actinobacterial isolates on growth performance and endogenous hormonal levels of mangroves. Therefore, we isolated endophytic plant growth-promoting (PGP) actinobacteria (PGPA) from mangrove roots and evaluated their potential as biological inoculants on mangrove seedlings under greenhouse and open-field nursery conditions. Seven salt-tolerant isolates had the ability to produce different levels of in vitro plant growth regulators (PGRs) and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase (ACCD) and to solubilize phosphorus. Accordingly, only one isolate, Streptomyces tubercidicus UAE1 (St), was selected based on its relative superiority in displaying multiple modes of action and in successfully colonizing mangrove tissues for 15 weeks....

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Research paper thumbnail of ROS generation and DNA damage accumulation in carnosol-treated MDA-MB-231 cells

<p>(<b>A</b>) Carnosol induces ROS generation in MDA-MB-231 cells. Cells were t... more <p>(<b>A</b>) Carnosol induces ROS generation in MDA-MB-231 cells. Cells were treated with indicated concentrations of carnosol for 24 h and ROS production was analyzed, as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109630#s2&quot; target="_blank">Material and Methods</a>, using DCFDA fluorescence stain. (<b>B</b>) time-course measurement of ROS generation in carnosol-treated MDA-MB-231 cells. Cells were treated with 100 µM carnosol and ROS generation was examined at different time-point (1, 3 and 6 h). (<b>C–D</b>) Concentration and time-dependent accumulation of γH2AX, marker of DNA damage, in carnosol-treated cells. DNA damage was evaluated by measuring, by Western blotting, the level γH2AX accumulation using anti-phospho-H2AX (ser 139) antibody.</p

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Research paper thumbnail of Light and electron microscopy analysis of carnosol-treated MDA-MB-231 cells

<p>(<b>A</b>) Micrograph of MDA-MB-231 cells, after 6 h incubation with various... more <p>(<b>A</b>) Micrograph of MDA-MB-231 cells, after 6 h incubation with various concentrations of carnosol. (<b>B</b>) Representative electron micrographs of untreated MDA-MB-231 cells (a) and MDA-MB-231 cells treated with 25 (b), 50 (c) and 100 (d) µM carnosol for 24 h.</p

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Research paper thumbnail of Carnosol induces Autophagy in MDA-B231 cells

<p>(<b>A</b>) Carnosol induces the formation of autophagic vacuoles MDA-MB 231 ... more <p>(<b>A</b>) Carnosol induces the formation of autophagic vacuoles MDA-MB 231 cells observed after 24 h post treatment. MDA-MB-231 cells were seeded at a density of 5×10<sup>4</sup> cells per well into 12-well plate followed by treatment with DMSO (vehicle) or 50 and 100 µM carnosol. Following treatment cells were washed and stained for autophagic vacuoles using the autophagy detection kit according to manufacturer's instructions. Fluorescent autophagic vacuoles were examined under Nikon Ti U fluorescence microscope. (<b>B</b>) Western blotting analysis of LC3II, p62(SQSTM1), Beclin-1 and pERK1/2 in carnosol-treated MD-MB 231 cells. Cells were treated with DMSO or increasing concentration of carnosol for 24 h, then whole cell proteins were extracted and subjected to Western blot analysis, as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109630#s2&quot; target="_blank">Materials and Methods</a>, for LC3II, 62(SQSTM1), Beclin1, pERK1/2 and β-actin (loading control) proteins. (<b>C</b>) carnosol induced autophagy is independent of Beclin1. MDA-MB-231 cells were transiently transfected with siRNA against Beclin1 for 72 hours followed by exposure to carnosol (50 µM) for 24 hours. Whole cell lysates were then probed for LC3II. (<b>D</b>) Time-course analysis, by Western blotting, of PARP cleavage and LC3II accumulation in carnosol-treated MDA-MB-231 cells. Cells were treated with 100 µM carnosol and proteins were extracted at the indicated time-point (3, 6, 24 and 48 h) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109630#s2&quot; target="_blank">Materials and Methods</a>.</p

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Research paper thumbnail of Biocontrol Potential of Endophytic Actinobacteria against Fusarium solani, the Causal Agent of Sudden Decline Syndrome on Date Palm in the UAE

Journal of Fungi, 2021

Thirty-one endophytic streptomycete and non-streptomycete actinobacteria were isolated from healt... more Thirty-one endophytic streptomycete and non-streptomycete actinobacteria were isolated from healthy date palm root tissues. In vitro screening revealed that the antifungal action of isolate #16 was associated with the production of cell-wall degrading enzymes, whereas with diffusible antifungal metabolites in isolate #28, albeit their production of volatile antifungal compounds. According to the 16S rRNA gene sequencing, isolates #16 and #28 were identified as Streptomyces polychromogenes UAE2 (Sp; GenBank Accession #: OK560620) and Streptomyces coeruleoprunus UAE1 (Sc; OK560621), respectively. The two antagonists were recovered from root tissues until 12 weeks after inoculation, efficiently colonized root cortex and xylem vessels, indicating that the date palm roots are a suitable habitat for these endophytic isolates. At the end of the greenhouse experiments, the development of sudden decline syndrome (SDS) was markedly suppressed by 53% with the application of Sp and 86% with Sc,...

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Research paper thumbnail of The Marine Endophytic Polyamine-Producing Streptomyces mutabilis UAE1 Isolated From Extreme Niches in the Arabian Gulf Promotes the Performance of Mangrove (Avicennia marina) Seedlings Under Greenhouse Conditions

Frontiers in Marine Science, 2021

Mangrove forests in the Arabian Gulf are under continuous threat. To increase plantations of gray... more Mangrove forests in the Arabian Gulf are under continuous threat. To increase plantations of gray mangrove (Avicennia marina) in the United Arab Emirates (UAE), 27 endophytic actinobacterial isolates obtained from mangrove roots were in vitro pre-screened to detect the polyamines (PAs) [putrescine (Put), spermidine (Spd), and spermine (Spm)]. We also determined the abilities of the endophytic PA-producing actinobacterial isolates in enhancing the growth of A. marina under greenhouse conditions. Although three highly PA-producing isolates were recovered from inside mangrove root tissues, Streptomyces mutabilis UAE1 constantly colonized root and stem inner tissues for 12 weeks, suggesting an endophytic association between this actinobacterial isolate and mangrove seedlings. When roots were inoculated with S. mutabilis, mangrove growth was remarkably enhanced under gnotobiotic and greenhouse conditions. This was evident from the significant (P < 0.05) increases in dry weight and len...

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Research paper thumbnail of Carnosol Induces ROS-Mediated Beclin1-Independent Autophagy and Apoptosis in Triple Negative Breast Cancer

PLoS ONE, 2014

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Research paper thumbnail of Rhus coriaria induces senescence and autophagic cell death in breast cancer cells through a mechanism involving p38 and ERK1/2 activation

Scientific Reports, 2015

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Research paper thumbnail of Coral Reef Reproduction and its Features in the Arabian Gulf (Jebel Ali, UAE)

International Journal of Environment and Sustainability, 2012

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Research paper thumbnail of Effect of licorice extract on the complications of diabetes nephropathy in rats

Drug and Chemical Toxicology, 2011

The aim of this study was to investigate the protective action of licorice in diabetic nephropath... more The aim of this study was to investigate the protective action of licorice in diabetic nephropathy in male rats. Diabetes was induced in male Wistar rats by using streptozotocin (60 mg/kg body weight). Daily oral ingestion (1 g/kg body weight) of licorice extract for 60 days after the onset of diabetes reversed the adverse effect of diabetes on rats. Licorice extract alleviated blood glucose levels, restored renal function, and attenuated body-weight loss. In addition, licorice extract modulated the adverse effect of diabetes on renal malondialdehyde, glutathione, superoxide dismutase, and catalase activity. Further, licorice extract restored the total antioxidant capacity of diabetic rat kidneys. The biochemical analyses were reinforced by histologic investigations, where focal segmental glomerulosclerosis, tubular damage, and hyperemic kidney were the histologic changes seen in diabetic, but not in treated, rats. In conclusion, the biochemical analysis and the histologic investigations of diabetic rat kidneys treated with licorice extract revealed that licorice may have a potential therapeutic effect for diabetes due to its antioxidant and -hyperglycemic properties.

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Research paper thumbnail of Morphological and molecular identification of airborne <i>Fusarium</i> propagules trapped in a maize field in Hungary

Acta Phytopathologica et Entomologica Hungarica, 2011

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Research paper thumbnail of Licorice extract ameliorates diabetic nephropathy in rats

Planta Medica, 2009

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Research paper thumbnail of Sorbitol-Induced Diabetic-Like Retinal Lesions in Rats: Microscopic Study

American Journal of Pharmacology and Toxicology, 2007

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Research paper thumbnail of Enhancing and Shifting the Reproduction Mode in Daphnia Carinata (king, 1853) Fed on Different Types of Powdered Food

The purpose of this study is to test the effect of four different powdered food such as Rice (R),... more The purpose of this study is to test the effect of four different powdered food such as Rice (R), Wheat (W), Chickpeas/Hummus (C), Spirulina (S) and a mixture of the previous feed (R+W+C/H+S) on parthenogenesis and resting eggs (ephippia) production in Daphnia carinata (King,1853) hatched from the desert of Abu Dhabi, UAE. The study continued for 21-days at temperature 19°C ± 2°C and under 12:12 hours light/dark conditions. The analysis of the experimental results has shown that test animals fed on a mixture of food resulted in significant (p 0.05) production in parthenogenesis in comparison to other food. Meanwhile, Chickpeas/Hummus and Wheat respond similarly and produce nearly the same number (p>0.05) of ephippia and parthenogenesis eggs in comparison to the Spirulina powder. However, the least number of resting eggs and parthenogenesis (p<0.05) has produced by Daphnia carinata fed on Rice powder. Therefore, we concluded that a mixture of highly nutritive feeds strongly ind...

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Research paper thumbnail of Isolation, Culture Procedures and Microscopy of the Mouse Peritoneal Cells for Assaying Phagocytosis: An Undergraduate Laboratory Experiment

To investigate phagocytosis, a class of undergraduate students isolated, cultured and fixed the m... more To investigate phagocytosis, a class of undergraduate students isolated, cultured and fixed the mouse peritoneal cells. We concluded that the mouse immune system has high efficiency to eliminate pathogens. Educational research has shown many advantages of using “hands-on” experiments which will help students carry out different experimental techniques and ultimately gain laboratory research skills. Skills that include preparing tissue culture media, handling animals and cell culture techniques, staining, and utilizing microscopes, will develop the knowledge needed to work in a laboratory, to produce a plan to undertake and test scientific hypotheses, and to emphasize future employability skills and attitudes. Key words: Active-learning strategies, peritoneal cells,phagocytosis, microscopy, mouse Introduction Many science education reports have shown that traditional lectures accompanied with “ready recipe” labs do not serve the needs of the student during his/her educational develop...

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Research paper thumbnail of Effectiveness of augmentative biological control depends on landscape context

Scientific Reports

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Research paper thumbnail of Effectiveness of Augmentative Biological Control of Streptomyces griseorubens UAE2 Depends on 1-Aminocyclopropane-1-Carboxylic Acid Deaminase Activity against Neoscytalidium dimidiatum

Journal of Fungi, Oct 20, 2021

To manage stem canker disease on royal poinciana, actinobacterial isolates were used as biologica... more To manage stem canker disease on royal poinciana, actinobacterial isolates were used as biological control agents (BCAs) based on their strong in vitro inhibitory effects against Neoscytalidium dimidiatum. Streptomyces griseorubens UAE2 and Streptomyces wuyuanensis UAE1 had the ability to produce antifungal compounds and cell-wall-degrading enzymes (CWDEs). Only S. griseorubens, however, restored the activity of 1-aminocyclopropane-1-carboxylate (ACC) deaminase (ACCD). In vivo apple
fruit bioassay showed that lesion development was successfully constrained by either isolates on fruits inoculated with N. dimidiatum. In our greenhouse and container nursery experiments, S. griseorubens showed almost complete suppression of disease symptoms. This was evident when the preventive treatment of S. griseorubens significantly (p < 0.05) reduced the numbers of conidia of N. dimidiatum
and defoliated leaves of royal poinciana seedlings to lesser levels than when S. wuyuanensis was applied, but comparable to control treatments (no pathogen). The disease management of stem canker was also associated with significant (p < 0.05) decreases in ACC levels in royal poinciana stems when S. griseorubens was applied compared to the non-ACCD-producing S. wuyuanensis. This study is the first to report the superiority of antagonistic actinobacteria to enhance their effectiveness
as BCAs not only for producing antifungal metabolites and CWDEs but also for secreting ACCD.

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Research paper thumbnail of Synthesis of New Chromene Derivatives Targeting Triple-Negative Breast Cancer Cells

Cancers

Breast cancer continues to be the leading cause of cancer-related deaths among women worldwide. T... more Breast cancer continues to be the leading cause of cancer-related deaths among women worldwide. The most aggressive type of breast cancer is triple-negative breast cancer (TNBC). Indeed, not only does TNBC not respond well to several chemotherapeutic agents, but it also frequently develops resistance to various anti-cancer drugs, including taxane mitotic inhibitors. This necessitates the search for newer, more efficacious drugs. In this study, we synthesized two novel chromene derivatives (C1 and C2) and tested their efficacy against a battery of luminal type A and TNBC cell lines. Our results show that C1 and C2 significantly and specifically inhibited TNBC cell viability but had no effect on the luminal A cell type. In addition, these novel compounds induced mitotic arrest, cell multinucleation leading to senescence, and apoptotic cell death through the activation of the extrinsic pathway. We also showed that the underlying mechanisms for these actions of C1 and C2 involved inhibi...

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Research paper thumbnail of Hypothetic model illustrating the differential effect of carnosol in MDA-MB-231 breast cancer cells

<p>Hypothetic model illustrating the differential effect of carnosol in MDA-MB-231 breast c... more <p>Hypothetic model illustrating the differential effect of carnosol in MDA-MB-231 breast cancer cells.</p

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Research paper thumbnail of Induction of caspases-mediated apoptosis by carnosol in the MDA-MB-231 cells

<p>(<b>A</b>) Carnosol induced apoptosis in the MDA-MB-231 cells. Annexin V bin... more <p>(<b>A</b>) Carnosol induced apoptosis in the MDA-MB-231 cells. Annexin V binding was carried out using Annexin V & Dead Cell kit (Millipore). Cells were treated with DMSO or various concentration of carnosol for 24 h. Detached and adherent cells were collected and stained with Annexin V and 7-AAD and then the events for early and late apoptotic cells were counted with the Muse Cell Analyzer as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109630#s2&quot; target="_blank">Materials and Methods</a>. (<b>B</b>) Western blot analysis of caspase 3, -9, and -8 activation, PARP cleavage and Bcl1 and Bax expression in MDA-MB-231 cells were treated with increasing concentrations of carnosol (25, 50 and 100 µM) for 24 h. (<b>C</b>) Carnosol induces the depolarization of mitochondrial membrane. Mitochondrial membrane potential (MMP) was assessed with the Muse Cell Analyzer using the Muse MitoPotential kit as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109630#s2&quot; target="_blank">Materials and Methods</a>. Data represent the mean ±SEM of at least 3 independent experiments. Student's t test was performed to determine the significance (*<i>p</i><0.05, **<i>p</i><0.01 and ***<i>p</i><0.005).</p

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Research paper thumbnail of The Multifarious Endophytic Actinobacterial Isolate, Streptomyces tubercidicus UAE1, Combined With the Seaweed Biostimulant Further Promotes Growth of Avicennia marina

Frontiers in Marine Science

Mangrove (Avicennia marina) is a “green lung” tree growing along the Arabian Gulf coastline in th... more Mangrove (Avicennia marina) is a “green lung” tree growing along the Arabian Gulf coastline in the United Arab Emirates. Here, we aimed to determine the impact of the application of a commercial seaweed extract (SWE) biostimulant and endophytic actinobacterial isolates on growth performance and endogenous hormonal levels of mangroves. Therefore, we isolated endophytic plant growth-promoting (PGP) actinobacteria (PGPA) from mangrove roots and evaluated their potential as biological inoculants on mangrove seedlings under greenhouse and open-field nursery conditions. Seven salt-tolerant isolates had the ability to produce different levels of in vitro plant growth regulators (PGRs) and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase (ACCD) and to solubilize phosphorus. Accordingly, only one isolate, Streptomyces tubercidicus UAE1 (St), was selected based on its relative superiority in displaying multiple modes of action and in successfully colonizing mangrove tissues for 15 weeks....

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Research paper thumbnail of ROS generation and DNA damage accumulation in carnosol-treated MDA-MB-231 cells

<p>(<b>A</b>) Carnosol induces ROS generation in MDA-MB-231 cells. Cells were t... more <p>(<b>A</b>) Carnosol induces ROS generation in MDA-MB-231 cells. Cells were treated with indicated concentrations of carnosol for 24 h and ROS production was analyzed, as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109630#s2&quot; target="_blank">Material and Methods</a>, using DCFDA fluorescence stain. (<b>B</b>) time-course measurement of ROS generation in carnosol-treated MDA-MB-231 cells. Cells were treated with 100 µM carnosol and ROS generation was examined at different time-point (1, 3 and 6 h). (<b>C–D</b>) Concentration and time-dependent accumulation of γH2AX, marker of DNA damage, in carnosol-treated cells. DNA damage was evaluated by measuring, by Western blotting, the level γH2AX accumulation using anti-phospho-H2AX (ser 139) antibody.</p

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Research paper thumbnail of Light and electron microscopy analysis of carnosol-treated MDA-MB-231 cells

<p>(<b>A</b>) Micrograph of MDA-MB-231 cells, after 6 h incubation with various... more <p>(<b>A</b>) Micrograph of MDA-MB-231 cells, after 6 h incubation with various concentrations of carnosol. (<b>B</b>) Representative electron micrographs of untreated MDA-MB-231 cells (a) and MDA-MB-231 cells treated with 25 (b), 50 (c) and 100 (d) µM carnosol for 24 h.</p

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Research paper thumbnail of Carnosol induces Autophagy in MDA-B231 cells

<p>(<b>A</b>) Carnosol induces the formation of autophagic vacuoles MDA-MB 231 ... more <p>(<b>A</b>) Carnosol induces the formation of autophagic vacuoles MDA-MB 231 cells observed after 24 h post treatment. MDA-MB-231 cells were seeded at a density of 5×10<sup>4</sup> cells per well into 12-well plate followed by treatment with DMSO (vehicle) or 50 and 100 µM carnosol. Following treatment cells were washed and stained for autophagic vacuoles using the autophagy detection kit according to manufacturer's instructions. Fluorescent autophagic vacuoles were examined under Nikon Ti U fluorescence microscope. (<b>B</b>) Western blotting analysis of LC3II, p62(SQSTM1), Beclin-1 and pERK1/2 in carnosol-treated MD-MB 231 cells. Cells were treated with DMSO or increasing concentration of carnosol for 24 h, then whole cell proteins were extracted and subjected to Western blot analysis, as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109630#s2&quot; target="_blank">Materials and Methods</a>, for LC3II, 62(SQSTM1), Beclin1, pERK1/2 and β-actin (loading control) proteins. (<b>C</b>) carnosol induced autophagy is independent of Beclin1. MDA-MB-231 cells were transiently transfected with siRNA against Beclin1 for 72 hours followed by exposure to carnosol (50 µM) for 24 hours. Whole cell lysates were then probed for LC3II. (<b>D</b>) Time-course analysis, by Western blotting, of PARP cleavage and LC3II accumulation in carnosol-treated MDA-MB-231 cells. Cells were treated with 100 µM carnosol and proteins were extracted at the indicated time-point (3, 6, 24 and 48 h) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109630#s2&quot; target="_blank">Materials and Methods</a>.</p

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Research paper thumbnail of Biocontrol Potential of Endophytic Actinobacteria against Fusarium solani, the Causal Agent of Sudden Decline Syndrome on Date Palm in the UAE

Journal of Fungi, 2021

Thirty-one endophytic streptomycete and non-streptomycete actinobacteria were isolated from healt... more Thirty-one endophytic streptomycete and non-streptomycete actinobacteria were isolated from healthy date palm root tissues. In vitro screening revealed that the antifungal action of isolate #16 was associated with the production of cell-wall degrading enzymes, whereas with diffusible antifungal metabolites in isolate #28, albeit their production of volatile antifungal compounds. According to the 16S rRNA gene sequencing, isolates #16 and #28 were identified as Streptomyces polychromogenes UAE2 (Sp; GenBank Accession #: OK560620) and Streptomyces coeruleoprunus UAE1 (Sc; OK560621), respectively. The two antagonists were recovered from root tissues until 12 weeks after inoculation, efficiently colonized root cortex and xylem vessels, indicating that the date palm roots are a suitable habitat for these endophytic isolates. At the end of the greenhouse experiments, the development of sudden decline syndrome (SDS) was markedly suppressed by 53% with the application of Sp and 86% with Sc,...

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Research paper thumbnail of The Marine Endophytic Polyamine-Producing Streptomyces mutabilis UAE1 Isolated From Extreme Niches in the Arabian Gulf Promotes the Performance of Mangrove (Avicennia marina) Seedlings Under Greenhouse Conditions

Frontiers in Marine Science, 2021

Mangrove forests in the Arabian Gulf are under continuous threat. To increase plantations of gray... more Mangrove forests in the Arabian Gulf are under continuous threat. To increase plantations of gray mangrove (Avicennia marina) in the United Arab Emirates (UAE), 27 endophytic actinobacterial isolates obtained from mangrove roots were in vitro pre-screened to detect the polyamines (PAs) [putrescine (Put), spermidine (Spd), and spermine (Spm)]. We also determined the abilities of the endophytic PA-producing actinobacterial isolates in enhancing the growth of A. marina under greenhouse conditions. Although three highly PA-producing isolates were recovered from inside mangrove root tissues, Streptomyces mutabilis UAE1 constantly colonized root and stem inner tissues for 12 weeks, suggesting an endophytic association between this actinobacterial isolate and mangrove seedlings. When roots were inoculated with S. mutabilis, mangrove growth was remarkably enhanced under gnotobiotic and greenhouse conditions. This was evident from the significant (P < 0.05) increases in dry weight and len...

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Research paper thumbnail of Carnosol Induces ROS-Mediated Beclin1-Independent Autophagy and Apoptosis in Triple Negative Breast Cancer

PLoS ONE, 2014

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Research paper thumbnail of Rhus coriaria induces senescence and autophagic cell death in breast cancer cells through a mechanism involving p38 and ERK1/2 activation

Scientific Reports, 2015

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Research paper thumbnail of Coral Reef Reproduction and its Features in the Arabian Gulf (Jebel Ali, UAE)

International Journal of Environment and Sustainability, 2012

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Research paper thumbnail of Effect of licorice extract on the complications of diabetes nephropathy in rats

Drug and Chemical Toxicology, 2011

The aim of this study was to investigate the protective action of licorice in diabetic nephropath... more The aim of this study was to investigate the protective action of licorice in diabetic nephropathy in male rats. Diabetes was induced in male Wistar rats by using streptozotocin (60 mg/kg body weight). Daily oral ingestion (1 g/kg body weight) of licorice extract for 60 days after the onset of diabetes reversed the adverse effect of diabetes on rats. Licorice extract alleviated blood glucose levels, restored renal function, and attenuated body-weight loss. In addition, licorice extract modulated the adverse effect of diabetes on renal malondialdehyde, glutathione, superoxide dismutase, and catalase activity. Further, licorice extract restored the total antioxidant capacity of diabetic rat kidneys. The biochemical analyses were reinforced by histologic investigations, where focal segmental glomerulosclerosis, tubular damage, and hyperemic kidney were the histologic changes seen in diabetic, but not in treated, rats. In conclusion, the biochemical analysis and the histologic investigations of diabetic rat kidneys treated with licorice extract revealed that licorice may have a potential therapeutic effect for diabetes due to its antioxidant and -hyperglycemic properties.

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Research paper thumbnail of Morphological and molecular identification of airborne <i>Fusarium</i> propagules trapped in a maize field in Hungary

Acta Phytopathologica et Entomologica Hungarica, 2011

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Research paper thumbnail of Licorice extract ameliorates diabetic nephropathy in rats

Planta Medica, 2009

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Research paper thumbnail of Sorbitol-Induced Diabetic-Like Retinal Lesions in Rats: Microscopic Study

American Journal of Pharmacology and Toxicology, 2007

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Research paper thumbnail of Enhancing and Shifting the Reproduction Mode in Daphnia Carinata (king, 1853) Fed on Different Types of Powdered Food

The purpose of this study is to test the effect of four different powdered food such as Rice (R),... more The purpose of this study is to test the effect of four different powdered food such as Rice (R), Wheat (W), Chickpeas/Hummus (C), Spirulina (S) and a mixture of the previous feed (R+W+C/H+S) on parthenogenesis and resting eggs (ephippia) production in Daphnia carinata (King,1853) hatched from the desert of Abu Dhabi, UAE. The study continued for 21-days at temperature 19°C ± 2°C and under 12:12 hours light/dark conditions. The analysis of the experimental results has shown that test animals fed on a mixture of food resulted in significant (p 0.05) production in parthenogenesis in comparison to other food. Meanwhile, Chickpeas/Hummus and Wheat respond similarly and produce nearly the same number (p>0.05) of ephippia and parthenogenesis eggs in comparison to the Spirulina powder. However, the least number of resting eggs and parthenogenesis (p<0.05) has produced by Daphnia carinata fed on Rice powder. Therefore, we concluded that a mixture of highly nutritive feeds strongly ind...

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Research paper thumbnail of Isolation, Culture Procedures and Microscopy of the Mouse Peritoneal Cells for Assaying Phagocytosis: An Undergraduate Laboratory Experiment

To investigate phagocytosis, a class of undergraduate students isolated, cultured and fixed the m... more To investigate phagocytosis, a class of undergraduate students isolated, cultured and fixed the mouse peritoneal cells. We concluded that the mouse immune system has high efficiency to eliminate pathogens. Educational research has shown many advantages of using “hands-on” experiments which will help students carry out different experimental techniques and ultimately gain laboratory research skills. Skills that include preparing tissue culture media, handling animals and cell culture techniques, staining, and utilizing microscopes, will develop the knowledge needed to work in a laboratory, to produce a plan to undertake and test scientific hypotheses, and to emphasize future employability skills and attitudes. Key words: Active-learning strategies, peritoneal cells,phagocytosis, microscopy, mouse Introduction Many science education reports have shown that traditional lectures accompanied with “ready recipe” labs do not serve the needs of the student during his/her educational develop...

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Research paper thumbnail of Effectiveness of augmentative biological control depends on landscape context

Scientific Reports

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Research paper thumbnail of Effectiveness of Augmentative Biological Control of Streptomyces griseorubens UAE2 Depends on 1-Aminocyclopropane-1-Carboxylic Acid Deaminase Activity against Neoscytalidium dimidiatum

Journal of Fungi, Oct 20, 2021

To manage stem canker disease on royal poinciana, actinobacterial isolates were used as biologica... more To manage stem canker disease on royal poinciana, actinobacterial isolates were used as biological control agents (BCAs) based on their strong in vitro inhibitory effects against Neoscytalidium dimidiatum. Streptomyces griseorubens UAE2 and Streptomyces wuyuanensis UAE1 had the ability to produce antifungal compounds and cell-wall-degrading enzymes (CWDEs). Only S. griseorubens, however, restored the activity of 1-aminocyclopropane-1-carboxylate (ACC) deaminase (ACCD). In vivo apple
fruit bioassay showed that lesion development was successfully constrained by either isolates on fruits inoculated with N. dimidiatum. In our greenhouse and container nursery experiments, S. griseorubens showed almost complete suppression of disease symptoms. This was evident when the preventive treatment of S. griseorubens significantly (p < 0.05) reduced the numbers of conidia of N. dimidiatum
and defoliated leaves of royal poinciana seedlings to lesser levels than when S. wuyuanensis was applied, but comparable to control treatments (no pathogen). The disease management of stem canker was also associated with significant (p < 0.05) decreases in ACC levels in royal poinciana stems when S. griseorubens was applied compared to the non-ACCD-producing S. wuyuanensis. This study is the first to report the superiority of antagonistic actinobacteria to enhance their effectiveness
as BCAs not only for producing antifungal metabolites and CWDEs but also for secreting ACCD.

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