Abdulrezzak Memon | Uşak Üniversitesi (original) (raw)

Papers by Abdulrezzak Memon

ISPEC Journal of Agricultural Sciences, 2024

Tissue culture, an essential technique in modern plant biology, offers promising avenues for the ... more Tissue culture, an essential technique in modern plant biology, offers promising avenues for the mass production of elite plant varieties for desirable traits. This study compared the effects of 0.1 mg L-1 NAA-0.25 mg L-1 BAP plant growth regulator combination using Cotyledon leaves (0.4 cm²) of three Eruca cultivars Estht 195, Estht 198, and Estht 201 of arugula (Eruca sativa Mill.) cultured for four and six weeks. Significant differences were noted among them for callus induction percentage, shoot induction percentage, and plant height (cm). Notably, var. Estht 201 indicated consistently improved performance, with a callus formation percentage of 61.83 % in 4 weeks and 74.05 % in 6 weeks. Similarly, Var. Estht 201 exhibited higher shoot induction (Week 4 67.45 %, Week 6:69.9 %), and plant height (Week 4:7.50 cm, Week 6:9.6 cm), throughout the experiment. These findings contributed to a deeper understanding of arugula micropropagation Dynamics. At the same time, this study has once again shown how important tissue culture micropropagation protocols are in terms of improving yield and quality as well as breeding studies.

Russian Journal of Plant Physiology, 2023

The Brassicaceae plant family includes some of the most economically important crops with wideran... more The Brassicaceae plant family includes some of the most economically important crops with wideranging adaptability for cultivation under various agro-climatic conditions. Among 25% of discovered heavy metal hyperaccumulators belong to this family. Brassica juncea (L.) Czern. hyperaccumulate metals in leaves and thus has a great potential for phytoremediation of metals from contaminated soils. In this study, B. juncea plants were grown in the soil at different zinc concentrations (0-500 μM). Plants showed no toxicity symptoms and accumulated a significant amount of Zn in the leaves. Gene expression analysis showed that increased Zn levels in the leaves trigger the expression of the HMA2 and HMA4 genes, suggesting that they play an essential role in Zn detoxification and sequestration at the subcellular level. Furthermore, the bioinformatics analysis of these transporters in B. juncea showed a significant similarity at gene and protein levels to that of HMA2 and HMA4 transporters in other members of the Brassicaceae family. The data indicates the importance of these transporters in Zn sequestration and detoxification in accumulator plants in Brassicaceae.

Heavy metal pollution adversely affects soil ecology, agricultural production, and groundwater qu... more Heavy metal pollution adversely affects soil ecology, agricultural production, and groundwater quality and ultimately harms the health of living organisms in the food chain. Soils or waters contaminated with heavy metals affect plant growth and yield and often lead to the production of harmful metabolites. Hyperaccumulator plants possess various cellular mechanisms responsible for detoxifying heavy metals from the cell and providing tolerance to metal stress. Much progress has been achieved in the last decade to elucidate the molecular mechanisms of metal tolerance and accumulation in accumulator plants. However, the detailed mechanism of hyperaccumulation in plants is still not well characterized. Therefore, there is an urgent need to develop practical tools and systems for studies on metal accumulations in plants at the molecular level. Genomic analysis of the Arabidopsis genome helped in the identification of many transporters. P 1B-heavy metal ATPases (HMAs) are one of the significant transporters belonging to the family of P-type ATPases involved in heavy metal homeostasis in the cell. Eight genes (AtHMA1-AtHMA8) have been identified in the Arabidopsis genome. This chapter intends to elucidate the heavy metal tolerance and accumulation mechanisms in the Brassicaceae family with the role of the HMAs (HMA1-HMA8) in response to heavy metal stress. The role and function of HMA transporters in A. thaliana have been clearly defined. In this paper, we utilized the genomic knowledge of Arabidopsis thaliana to understand the role of these metal transporter ATPases in other species in Brassicaceae. We performed the phylogenetic analysis, multiple sequence alignments, 3D structure prediction, and validation to investigate the interacting proteins present in different plant species, including agriculturally important crop species in Brassica. Studies on these protein-protein interactions are most important to understand the complexity of the function of HMA proteins. This chapter provides the recent development in heavy metal transporting ATPases in metal transport and translocation in the accumulator

Turkish Journal of Agriculture - Food Science and Technology, 2022

Turkish Journal of Agriculture: Food Science and Technology, Feb 22, 2021

The microbes that live in and on plants (the plant microbiome) are critical for plant health and ... more The microbes that live in and on plants (the plant microbiome) are critical for plant health and exert their influence by facilitating the nutrient acquisition, regulating plant hormone levels, and helping to withstand pathogen attack. Plants are meta-organisms that are associated with complex microbiomes. The majority of the microorgansims including epiphytes and endophytes generally play a significant role in providing essential nutrients to the plants where they live. In addition, plant microbe interaction affects the content of secondary metabolites and their derivatives in the host plant. In this review article we summarizes the interaction of the plant and microbe interaction especially the microorganisms of the rhizosphere and their effect on the secondary metabolites level in plants. The current knowledge of the plant-microbe interaction at molecular level are also being reviewed in brief.

Plant Biology, Jul 1, 2011

ARF1 (ADP‐ribosylation factor 1) and SAR1 (secretion‐associated RAS super family) are involved in... more ARF1 (ADP‐ribosylation factor 1) and SAR1 (secretion‐associated RAS super family) are involved in the formation and budding of vesicles throughout plant endomembrane systems. The molecular mechanisms of this transport have been studied extensively in mammalian and yeast cells. However, very little is known about the mechanisms of coat protein complex (COP) formation and recruitment of COP‐vesicle cargoes in plants. To provide insights into vesicular trafficking in Pisum sativum L., we investigated mRNA and protein expression patterns of ARF1 and SAR1 in roots and shoots at early growth stages and in the de‐etiolation process. We showed that ARF1 was concentrated mostly in the crude Golgi fractions, and SAR1 was concentrated predominantly in the crude ER fractions of de‐etiolated shoots. ARF1 and SAR1 proteins were several times more abundant in shoots relative to roots. In total protein homogenates, the expression level of SAR1 and ARF1 was higher in shoots of dark‐grown pea plants than light‐grown plants. In contrast, ARF1 was higher in roots of light‐grown pea relative to roots of dark‐grown pea. With ageing, the ARF1 mRNA in roots was reduced, while SAR1 expression increased. Unlike ARF1 transcripts, ARF1 protein levels did not fluctuate significantly in root and shoot tissue during early development. The relative abundance of SAR1 protein in root tissues may suggest a high level of vesicular transport from the ER to the Golgi. Experimental results suggested that white light probably affects the regulation of ARF1 and SAR1 protein levels. On the other hand, short‐term white light affects SAR1 but not ARF1.

日本土壌肥料学会講演要旨集, Mar 25, 1983

日本土壌肥料学会講演要旨集, Jul 25, 1979

日本土壌肥料学会講演要旨集, Mar 25, 1980

Plant Physiology, Supplement; (USA), Apr 1, 1989

Springer eBooks, 2016

Several hyperaccumulator plant species especially the species in Brassicaceae have been extensive... more Several hyperaccumulator plant species especially the species in Brassicaceae have been extensively investigated for their metal accumulation and detoxification. For example, Arabidopsis halleri, Noccaea caerulescens (formerly Thlaspi caerulescens), and Brassica nigra have enhanced our understanding of the physiological, molecular, and genetic basis of metal hyperaccumulation and associated hypertolerance. A number of regulatory mechanisms have been developed by metal hyperaccumulator plants for their survival in metal-polluted environment. In the last decade, with the development of advanced technologies most of the information about heavy metal stress in plants has been obtained through genome sequence, transcriptome, metabolome, and proteome studies. For example, through such techniques, it has been possible to identify numerous putative genes involved in the response to metal stress. A number of membrane transporter gene families have been found in accumulator plants such as ZIP (ZRT, IRT-like protein), NRAMP (natural resistance-associated macrophage protein), YSL (Yellow-stripe-like transporter), NAS (nicotinamine synthase), SAMS (S-adenosyl-methionine synthetase), FER (ferritin Fe (III) binding), CDF (cation diffusion facilitator), HMA (heavy metal ATPase), and IREG (iron-regulated transporter) families which are predicted to be involved in the cellular uptake and transport in plants. HMAs are particularly interesting and according to many recent studies they have been shown a key player in the metal hyperaccumulation. In this regard, we have analyzed the gene expression data of model crop plants in Brassicaceae family by searching several databases available online. The publicly available online resources for these plants from websites such as http://www.ncbi.nih.gov, http://www.tigr.org, http://www.brassica.info, and related sites were searched to collect nucleotide sequences that encode heavy metal ATPases and transporter protein homologues. The criterion observed in this research is that the sequences of different metal-induced genes have functional and evolutionary similarities among species. Our hypothesis is that the functionally related sequences of the genes from different species or organisms will be having conserved pattern or motif which will be possibly related to hyperaccumulation of heavy metals. Here, I will overview these findings and highlight their contribution to the field of plant metal homeostasis, and will discuss the emerging avenues of -omics technologies and their impact in understanding the mechanisms of metal accumulation and tolerance.

Journal of Biological Chemistry, Sep 1, 1993

Journal of Biological Chemistry, Sep 1, 1990

Journal of Experimental Botany, 1985

Plant Physiology, Mar 1, 1984

Influx and accumulation of K' in barley (Hordeum vulgare L. cv Fergus) roots were measured at two... more Influx and accumulation of K' in barley (Hordeum vulgare L. cv Fergus) roots were measured at two temperatures (10°C and 20C) in plants which had been grown with roots and shoots at 20°C (HT plants), with roots and shoots at 10°C (LT plants), and with roots at 10°C and shoots at 20°C (DT plants). Under conditions where K' was in limited supply during the prior growth period, K' influx and accumulation were consistently higher in roots of DT and LT plants than in those of HT plants. Thus, it would appear that this low temperature response is not limited specifically to conditions in which temperature differentials are maintained between roots and shoots. Nevertheless, it was generally the case that increases of influx were larger in DT and LT plants so that the temperature differentials may intensify the low temperature response. When K4 influx was examined over a wide range of root IK+, it was seen that the characteristic reduction of influx associated with increased internal IKIl was substantially greater in HT than DT or LT plants. Transfer of plants grown under HT conditions to DT or LT regimes led to both short-term and long-term adjustments of influx. The former became apparent within 6 hours of exposure to the new conditions and decayed within minutes of transfer back to 20°C. The long-term adjustments were only apparent after prolonged exposure (days) to the lower root temperature and these did not decay as rapidly. Regardless of shoot temperature, the transfer of roots from 20°C to 10°C caused a gradual increase of root IK@] so that 4 days later LT and DT roots contained, respectively, 53.3 and 49.83 micromoles per gram compared to 17.82 micromoles per gram for roots maintained at 20°C.

Biochemical and Biophysical Research Communications, Jun 1, 1993

Biochimica Et Biophysica Acta - Biomembranes, Oct 1, 2004

On page 20, in the fourth and fifth line of the caption of Fig. 5, the colours in the figure are ... more On page 20, in the fourth and fifth line of the caption of Fig. 5, the colours in the figure are incorrectly referred. Sec23 is depicted in light green in the upper part of the Fig. 5; Sec24 is depicted in dark green in the lower part.