Mahbub Hasan | The University of Asia Pacific Dhaka Bangladesh (original) (raw)

Papers by Mahbub Hasan

Polymer Composites, 2015

Hybrid natural fiber polymer nanocomposites were prepared using various natural fibers (kenaf, co... more Hybrid natural fiber polymer nanocomposites were prepared using various natural fibers (kenaf, coir, and wood), polypropylene, and montmorillonite nanoclay through the hot compression method. The effects of fiber hybridization and nanoclay content on the physicomechanical and biodegradable properties of the synthesized composites were investigated. Fourier-transform infrared and scanning electron microscopic analyses indicated that the structure and surface morphology of composites were transformed after fiber hybridization and the subsequent nanoclay incorporation. X-ray diffraction pattern revealed that the percent crystallinity of hybrid nanocomposites significantly increased. Furthermore, the tensile strength and tensile modulus also significantly improved for the hybrid nanocomposites due to the addition of montmorillonite nanoclay. The biodegradability and water absorption tests were conducted. The results show that biodegradability of the nanocomposites decreased and water absorption increased due to the addition of montmorillonite nanoclay.

Canadian Journal of Infectious Diseases and Medical Microbiology

Background. Multidrug-resistant (MDR) clones of Klebsiella pneumoniae (Kpn) have been increasingl... more Background. Multidrug-resistant (MDR) clones of Klebsiella pneumoniae (Kpn) have been increasingly documented in community-acquired and nosocomial infections all around the globe. Extended-spectrum β-lactamases (ESBLs) are a rapidly evolving group of β-lactamase enzymes derived from SHV genes by mutations. This research work aimed to investigate and analyze the widespread prevalence of Kpn antibiotic resistance in different areas of the southern part of Bangladesh. Methods. This particular study was executed and implemented by using 501 clinical samples or isolates from two different hospitals in Chattogram. The disk diffusion method was used to detect Kpn’s sensitivity to 16 antibiotics in a drug susceptibility test. By using the PCR technique, the widespread prevalence of antibiotic-resistant gene blaSHV-11 was studied. Sequencing along with phylogenetic analysis was utilized to verify isolates with the blaSHV-11 gene. Results. Almost all of the Kpn isolates were spotted to be ant...

Background: During an outbreak, the health-seeking behaviour (HSB) of patients plays a vital role... more Background: During an outbreak, the health-seeking behaviour (HSB) of patients plays a vital role to mitigate the disease spread. Poor HSB may significantly increase mortality and complicate contact tracing. In our study, we aimed to assess the status of HSB among the educated young adults of Bangladesh.Methods: A cross-sectional study was conducted online in June-July 2020 when the country was under strict social distancing measures. A snowball sampling method was employed to capture the suspected COVID-19 patients who did not undergo the COVID-19 test. Descriptive and inferential analyses were performed with statistical significance defined as p < 0.05. Results: Among the 390 participants, more male (79.5%) and urban-dwelling residents (80.3%) participated in the study. About 45% of the participants had a bachelor’s degree followed by 25.9% of post-graduation degrees such as master’s and Ph.D. Common symptoms included fever (77.7%), cough (50.5%), headache (46.2%), body pain (3...

Background: High levels of vitamin D deficiency are commonly reported even in regions with abunda... more Background: High levels of vitamin D deficiency are commonly reported even in regions with abundant sunshine. This necessitates a comprehensive understanding of the determinants that influence sun exposure practices. As the primary source of health-related knowledge for the general public, the attitude of the healthcare professionals towards sunlight and their awareness related to vitamin D deficiency can be critical in this regard. Methods: A cross-sectional survey was conducted among 2,242 physicians, intern doctors, and senior medical students in Bangladesh from October 2019 to February 2020. A pre-tested structured questionnaire (containing twelve close-ended questions) was used. The perceptions of health risks due to sun exposure, and basic knowledge of the physiological and epidemiological aspects of vitamin D deficiency were tested. Results: An overall negative attitude towards sunlight in the context of Bangladesh was highlighted – 68% participants thought regular sun exposu...

PLOS ONE, 2021

Prevalence of toxigenic Vibrio cholerae O1 in aquatic reservoirs in Bangladesh apparently increas... more Prevalence of toxigenic Vibrio cholerae O1 in aquatic reservoirs in Bangladesh apparently increases coinciding with the occurrence of seasonal cholera epidemics. In between epidemics, these bacteria persist in water mostly as dormant cells, known as viable but non-culturable cells (VBNC), or conditionally viable environmental cells (CVEC), that fail to grow in routine culture. CVEC resuscitate to active cells when enriched in culture medium supplemented with quorum sensing autoinducers CAI-1 or AI-2 which are signal molecules that regulate gene expression dependent on cell density. V. cholerae O1 mutant strains with inactivated cqsS gene encoding the CAI-1 receptor has been shown to overproduce AI-2 that enhance CVEC resuscitation in water samples. Since V. cholerae non-O1 non-O139 (non-cholera-vibrios) are abundant in aquatic ecosystems, we identified and characterized naturally occurring variant strains of V. cholerae non-O1 non-O139 which overproduce AI-2, and monitored their co-...

International Journal of Bioassays, 2015

Nanotechnology exhibits a tremendous potential in agriculture and its allied fields like, aquacul... more Nanotechnology exhibits a tremendous potential in agriculture and its allied fields like, aquaculture and fisheries. It is the new tools for aquaculture, fish biotechnology, fish genetics, fish reproduction and aquatic health etc. Nanotechnology tools like nanomaterials, nanosensors, DNA nanovaccines, Gene delivery, Drug delivery, Fluorescent biological labels, Probing of DNA structure, Bio detection of pathogens, Separation and purification of biological molecules and cells, antioxidant activity, antimicrobial activity cell toxicity etc. have the potential role to solve many problems like animal health, production, reproduction, prevention and treatment of diseases. It may presume that in the recent years, nanotechnology research will reform the science and technology and will help to boost up the livestock production in future. Nanotechnology applications in the fish processing industry can also be utilized to detect fish bacteria in packaging, produce stronger flavors, colour qua...

Mymensingh medical journal : MMJ, 2021

Danis-Weber type-B ankle fracture is one of the most common injuries in young and active individu... more Danis-Weber type-B ankle fracture is one of the most common injuries in young and active individual. This fracture offers a considerable challenge to orthopedic surgeon. Though there are several options for treating such type of fracture, ORIF by Pre-contoured Distal Fibular Locking Plate is the preferred option in the recent years. This descriptive type of observational study was performed from July 2017 to June 2019 in NITOR. Thirty (30) patients, 22 male and 8 female with an average age of 39 years with Danis-Weber type-B ankle fracture underwent ORIF by Pre-contoured Distal Fibular Locking Plate for fractured fibula and ORIF by 4.0mm cannulated cancellous screw for fractured medial malleolus. All the patients were initially managed by analgesic and short leg posterior slab. Average follow up was 24 weeks. Final outcome was assessed by AOFAS score. The main cause of injury was RTA (56.67%). Mean operation time was 1.2 hours. Mean duration of Hospital stay was 16.43±1.73 days. Sup...

BackgroundSouth Asian countries including Bangladesh have been struggling to control the COVID-19... more BackgroundSouth Asian countries including Bangladesh have been struggling to control the COVID-19 pandemic despite imposing months of lockdown and other public health measures (as of June 30, 2020). In-depth epidemiological information from these countries is lacking. From the perspective of Bangladesh, this study aims to understand the epidemiological features and gaps in public health preparedness.MethodThis study used publicly available data (8 March-30 June 2020) from the respective health departments of Bangladesh and Johns Hopkins University Coronavirus Resource Centre. Descriptive statistics was used to report the incidence, case fatality rates (CFR), and trend analysis. Spatial distribution maps were created using ArcGIS Desktop. Infection dynamics were analyzed via SIR models.FindingsIn 66 days of nationwide lockdown and other public health efforts, a total of 47,153 cases and 650 deaths were reported. However, the incidence was increased by around 50% within a week after r...

Md. Mahbub Hasan University of Chittagong Khaza Md. Kapil Uddin University of Chittagong Syed Moh... more Md. Mahbub Hasan University of Chittagong Khaza Md. Kapil Uddin University of Chittagong Syed Mohammad Lokman University of Chittagong Adnan Mannan University of Chittagong Enayetur Raheem Biomedical Research Foundation, Bangladesh Shahed Ahmad Chowdhury Chittagong Medical College Mohammad Sorowar Hossain (  sorowar.hossain@brfbd.org ) Biomedical Research Foundation https://orcid.org/0000-0001-7143-2909

Orphanet Journal of Rare Diseases, 2021

Background Thalassaemia, a hereditary haemoglobin disorder, is a major public health concern in s... more Background Thalassaemia, a hereditary haemoglobin disorder, is a major public health concern in some parts of the world. Although Bangladesh is in the world’s thalassaemia belt, the information on this disease is scarce. Additionally, the awareness of this life threatening, but potentially preventable disease is surprisingly poor. However, mass awareness is pivotal for the development of an effective preventive strategy. In this context, the understanding of parental perspectives is essential to grasp the magnitude of the problem. Therefore, this study aimed to investigate the parental knowledge gaps and perceptions regarding thalassemia, the barriers confronted by the parents for caring for their thalassaemic children and their attitude to prenatal screening and prenatal diagnosis. Methods This cross-sectional study was conducted between January 2018 and December 2018 at a dedicated thalassemia hospital located in Dhaka. A structured questionnaire was used for face-to-face intervie...

BackgroundSouth Asian countries including Bangladesh have been struggling to control the COVID-19... more BackgroundSouth Asian countries including Bangladesh have been struggling to control the COVID-19 pandemic despite imposing months of lockdown and other public health measures. In-depth epidemiological information from these countries is lacking. From the perspective of Bangladesh, this study aims at understanding the epidemiological features and gaps in public health preparedness and risk communication. MethodsThe study used publicly available data of seven months (8 March 2020–10 September 2020) from the respective health departments of Bangladesh and Johns Hopkins University Coronavirus Resource Centre. Human mobility data were obtained from Google COVID-19 Community Mobility Reports. Spatial distribution maps were created using ArcGIS Desktop. Descriptive statistics was used to report the incidence, case fatality rates (CFR), and trend analysis. Results Despite nationwide lockdown, an increase in human mobility linked to specific public events was observed. During this period, a...

Corona Virus Disease-2019 (COVID-19) warrants comprehensive investigations of publicly available ... more Corona Virus Disease-2019 (COVID-19) warrants comprehensive investigations of publicly available Severe Acute Respiratory Syndrome-CoronaVirus-2 (SARS-CoV-2) genomes to gain new insight about their epidemiology, mutations and pathogenesis. Nearly 0.4 million mutations were identified so far in ∼60,000 SARS-CoV-2 genomic sequences. In this study, we compared 207 of SARS-CoV-2 genomes reported from different parts of Bangladesh and their comparison with 467 globally reported sequences to understand the origin of viruses, possible patterns of mutations, availability of unique mutations, and their apparent impact on pathogenicity of the virus in victims of Bangladeshi population. Phylogenetic analyses indicates that in Bangladesh, SARS-CoV-2 viruses might arrived through infected travelers from European countries, and the GR clade was found as predominant in this region. We found 2602 mutations including 1602 missense mutations, 612 synonymous mutations, 36 insertions and deletions with...

Malaysian Journal on Composites Science and Manufacturing, 2020

Nowadays, NR/EPDM have drawn great attention to most researchers to be utilized in an engine moun... more Nowadays, NR/EPDM have drawn great attention to most researchers to be utilized in an engine mounting. In service, majority of structures and components are subjected to service condition such as heat, liquids and stress of dynamic loading. This research is an effort to compare the thermal effects on physical and mechanical properties of NR/EPDM blend and NR/EPDM filled graphene nanoplatelets (GNPs) for the potential usage in the engine mounting. The blends and nanocomposites were prepared through a melt compounding using a Banbury Internal Mixer, exposed to thermal cycles and tensile tested. The materials were heated alternately for 10 minutes at 60℃ and 120℃ for 0, 35, 70 and 150 cycles. Thermally aged NR/EPDM nanocomposites showed higher performance under tensile stress if compared to NR/EPDM blend. The tensile strength and elastic modulus were consistently 40% higher than the strength of the blend. This is due to the reinforcing effects of GNPs and their good interaction with the matrix. The reduction of tensile properties was due to the embrittlement effect and in line with the increase in amorphous phases from the broadening of XRD spectra. This has proven that the NR/EPDM nanocomposites can withstand thermal cycles, oils and fluctuating stress better than NR/EPDM blend and exhibited higher potential to be utilized as the engine mount material.

Journal of Food Science and Engineering, 2012

Functional properties of a food depends on its capacity to act as a natural antioxidant, by scave... more Functional properties of a food depends on its capacity to act as a natural antioxidant, by scavenging lipid peroxides and also by the ability of the particular food component to bind fat and sugars efficiently. This study was undertaken to primarily screening out the functional properties of different Bangladeshi food. In this study, the antioxidant properties, total phenolic content, flavonoid and lipid peroxide contents of eight commonly used fruits and vegetables were examined. The ethanol extract of fruits including Amra (Hog Plum-Spondias dulcis), Jambura (Grape Fruit-Citrus maxima), Anaros (Pineapple-Ananas comosus), and Kamranga (Carambola-Averrhoa carambola), and the vegetables including Kolmi Shak (Water spinach-Ipomoea aquatica), Lal Shak (Red amaranth-Amaranthus gangeticus), Data Shak (Stem amaranth-Amaranthus lividus), Korola (Gourd-Momordica charantia) were examined. Both the fat and sugar binding capacity was found the highest in Anaros and lowest in Amra. Mortality rate of Brine shrimp (Artemia salina) nauplii in brine shrimp lethality bioassay at different concentration of fruits and vegetables extract showed that Jambura has the highest cytotoxic effect, killing 100% of the brine shrimp at a very low concentration (0.01 mg/mL), compared to the effect of positive control (0.5 mg/mL of the Vincristine sulfate). In contrast, Lalshak showed the lowest (0% and 30% mortality) in vitro cytotoxic effect at both 0.01 and 0.1 mg/mL concentration. The Lal shak and Anaros extracts significantly reduced lipid peroxide (LPO) levels in the liver tissue homogenates. However, regarding the flavonoids content and phenolic content, Kamranga and Lal shak extracts showed the highest flavonoid contents, with mean values of 41.36 and 41.26 mg/g, respectively. Anaros and Jambura showed the lowest flavonoid contents, with mean values of 0.5 and 9.3 mg/g respectively. The phenolic contents as expressed as gallic acid equivalent (GAE) mg/g extracts showed that Kolmi shak had the highest amount of phenolic compounds (59.33 ± 3.72 mg/g of GAE) and Anaros has the lowest phenolic contents (11.00 ± 1.33 mg/g of GAE). Therefore, these primary findings revealed enormous potentiality of commonly consumed Bangladeshi fruits and vegetables in health sectors.

The newly identified SARS-CoV-2 has now been reported from around 183 countries with more than a ... more The newly identified SARS-CoV-2 has now been reported from around 183 countries with more than a million confirmed human cases including more than 68000 deaths. The genomes of SARS-COV-2 strains isolated from different parts of the world are now available and the unique features of constituent genes and proteins have gotten substantial attention recently. Spike glycoprotein is widely considered as a possible target to be explored because of its role during the entry of coronaviruses into host cells. We analyzed 320 whole-genome sequences and 320 spike protein sequences of SARS-CoV-2 using multiple sequence alignment tools. In this study, 483 unique variations have been identified among the genomes including 25 non-synonymous mutations and one deletion in the spike protein of SARS-CoV-2. Among the 26 variations detected, 12 variations were located at the N-terminal domain and 6 variations at the receptor-binding domain (RBD) which might alter the interaction with receptor molecules. ...

Orphanet Journal of Rare Diseases, 2020

Background Thalassaemia is a potentially life-threatening yet preventable inherited hemoglobin di... more Background Thalassaemia is a potentially life-threatening yet preventable inherited hemoglobin disorder. Understanding local socio-cultural context and level of public awareness about thalassaemia is pivotal for selecting effective prevention strategies. This study attempted to assess knowledge and perceptions about thalassaemia among college students in Bangladesh. Methods A supervised cross-sectional survey was conducted on 1578 college students using a self-administered structured questionnaire. The survey took place from 15 February 2018 to 17 March 2018 in the Jamalpur district in Bangladesh. Besides the attitude-related questions, the study asked a total of 12 knowledge-related questions, which were scored on a scale of 0–12 points. Results Over two-thirds (67%) of the college students had never heard of thalassaemia. The urban-rural dichotomy was observed among those familiar with the term; (46.4% from urban vs. 25.8% from rural colleges). A similar pattern was observed for k...

Open Journal of Medical Microbiology, 2018

Enterobacteriaceae are important human pathogens that cause many food and waterborne illness. Rap... more Enterobacteriaceae are important human pathogens that cause many food and waterborne illness. Rapid emergence of multi-drug resistant bacteria in Bangladesh has become a serious problem. Phage-host interaction is now considered as the major driving force for the conversion of non-pathogenic bacteria to pathogenic ones. Evolution of highly pathogenic and antibiotic resistant bacteria largely depends upon the horizontal gene transfer by means of plasmid, megaplasmid and bacteriophages. Conversely, bacteria may acquire a novel defence mechanism called CRISPR (Clustered regularly interspersed short palindromic repeats) that can restrict horizontal transfer of plasmids and bacteriophages to limit the spread of antibiotic resistance genes among bacterial species. In this study, twenty bacterial strains were isolated from water of different medical waste and Buriganga river. Therefore, CRISPR locus was investigated following various biochemical and molecular analysis of those bacterial isolates. Identification of the bacterial isolates was conducted by Polymerase Chain Reaction (PCR) based assay of 16S rDNA extracted from those isolated strains. Results indicated that most strains were identified as Proteus mirabilis and Citrobacter freundii which mainly cause septicemia, and pneumonia in human. Thereafter, antibiogram of these strains was performed by using 11 different antibiotic discs where bacterial isolates from medical drainage system showed more resistant to antibiotics than the river

ABSTRACTMajor intrinsic proteins (MIPs), commonly known as aquaporins, facilitate the membrane di... more ABSTRACTMajor intrinsic proteins (MIPs), commonly known as aquaporins, facilitate the membrane diffusion of water and some other non- polar solutes. MIPs might be involved in host-pathogen interactions. Herein, we identified 17, 24, 27, 19, 19, and 22 full-length MIPs, respectively, in the genomes of six Phytophthora species, P. infestans, P. parasitica, P. sojae, P. ramorum, P. capsici, and P. cinnamomi. These Phytophthora species are devastating plant pathogens and members of oomycetes, a distinct lineage of fungus-like eukaryotic microbes. Phylogenetic analysis showed that the Phytophthora MIPs (PMIPs) formed a completely distinct clade from their counterparts in other taxa and were clustered into nine subgroups. Sequence and structural properties indicated that the primary selectivity-related constrictions, including aromatic arginine (ar/R) selectivity filter and Froger’s positions in PMIPs were distinct from those in other taxa. The substitutions in the conserved Asn-Pro-Ala m...

The American Journal of Tropical Medicine and Hygiene, 2019

Chikungunya (CHIK) has emerged as a major public health concern worldwide. Recently, atypical man... more Chikungunya (CHIK) has emerged as a major public health concern worldwide. Recently, atypical manifestations are drawing special attention because these might be associated with various complications. Information on atypical manifestations of CHIK is still limited. Here, we analyzed a dataset of 1,326 cases from our recent Dhaka outbreak study to explore the demographics and distributions of atypical manifestations. About 80% of cases reported at least one atypical symptom. Among all atypical symptoms, the most common and unique atypical symptom was joint pain before fever (90.2%), occurred predominantly in female respondents. Other common symptoms included red eye (68.2%), oral ulcer (37.7%), and dermatological manifestations (27.1%). More than two-thirds of patients reported multiple atypical symptoms. Atypical manifestations were not significantly different across age groups, except ocular complications. This study would be an important resource for clinicians and epidemiologists...

PloS one, 2018

Type 2 diabetes mellitus is a multifactorial metabolic disorder caused by environmental factors a... more Type 2 diabetes mellitus is a multifactorial metabolic disorder caused by environmental factors and has a strong association with hereditary issues. These hereditary issues result in an imbalance in CD4+T cells and a decreased level of naïve CD4+T cells, which may be critical in the pathogenesis of type 2 diabetes. Transcription factors GATA3 and STAT4 mediate the cytokine-induced development of naïve T cells into Th1 or Th2 types. In the present study, genetic analyses of GATA3 SNP rs3824662 and STAT4 SNP rs10181656 were performed to investigate the association of allelic and genotypic variations with the risk of T2D in the Bangladeshi population. A total of 297 unrelated Bangladeshi patients with type 2 diabetes and 247 healthy individuals were included in the study. The allelic and genotypic frequencies of rs10181656 located in the STAT4 gene were not found to be associated with risk of type 2 diabetes. The GATA3 rs3824662 T allele and mutant TT genotype had a significant associa...

Polymer Composites, 2015

Hybrid natural fiber polymer nanocomposites were prepared using various natural fibers (kenaf, co... more Hybrid natural fiber polymer nanocomposites were prepared using various natural fibers (kenaf, coir, and wood), polypropylene, and montmorillonite nanoclay through the hot compression method. The effects of fiber hybridization and nanoclay content on the physicomechanical and biodegradable properties of the synthesized composites were investigated. Fourier-transform infrared and scanning electron microscopic analyses indicated that the structure and surface morphology of composites were transformed after fiber hybridization and the subsequent nanoclay incorporation. X-ray diffraction pattern revealed that the percent crystallinity of hybrid nanocomposites significantly increased. Furthermore, the tensile strength and tensile modulus also significantly improved for the hybrid nanocomposites due to the addition of montmorillonite nanoclay. The biodegradability and water absorption tests were conducted. The results show that biodegradability of the nanocomposites decreased and water absorption increased due to the addition of montmorillonite nanoclay.

Canadian Journal of Infectious Diseases and Medical Microbiology

Background. Multidrug-resistant (MDR) clones of Klebsiella pneumoniae (Kpn) have been increasingl... more Background. Multidrug-resistant (MDR) clones of Klebsiella pneumoniae (Kpn) have been increasingly documented in community-acquired and nosocomial infections all around the globe. Extended-spectrum β-lactamases (ESBLs) are a rapidly evolving group of β-lactamase enzymes derived from SHV genes by mutations. This research work aimed to investigate and analyze the widespread prevalence of Kpn antibiotic resistance in different areas of the southern part of Bangladesh. Methods. This particular study was executed and implemented by using 501 clinical samples or isolates from two different hospitals in Chattogram. The disk diffusion method was used to detect Kpn’s sensitivity to 16 antibiotics in a drug susceptibility test. By using the PCR technique, the widespread prevalence of antibiotic-resistant gene blaSHV-11 was studied. Sequencing along with phylogenetic analysis was utilized to verify isolates with the blaSHV-11 gene. Results. Almost all of the Kpn isolates were spotted to be ant...

Background: During an outbreak, the health-seeking behaviour (HSB) of patients plays a vital role... more Background: During an outbreak, the health-seeking behaviour (HSB) of patients plays a vital role to mitigate the disease spread. Poor HSB may significantly increase mortality and complicate contact tracing. In our study, we aimed to assess the status of HSB among the educated young adults of Bangladesh.Methods: A cross-sectional study was conducted online in June-July 2020 when the country was under strict social distancing measures. A snowball sampling method was employed to capture the suspected COVID-19 patients who did not undergo the COVID-19 test. Descriptive and inferential analyses were performed with statistical significance defined as p < 0.05. Results: Among the 390 participants, more male (79.5%) and urban-dwelling residents (80.3%) participated in the study. About 45% of the participants had a bachelor’s degree followed by 25.9% of post-graduation degrees such as master’s and Ph.D. Common symptoms included fever (77.7%), cough (50.5%), headache (46.2%), body pain (3...

Background: High levels of vitamin D deficiency are commonly reported even in regions with abunda... more Background: High levels of vitamin D deficiency are commonly reported even in regions with abundant sunshine. This necessitates a comprehensive understanding of the determinants that influence sun exposure practices. As the primary source of health-related knowledge for the general public, the attitude of the healthcare professionals towards sunlight and their awareness related to vitamin D deficiency can be critical in this regard. Methods: A cross-sectional survey was conducted among 2,242 physicians, intern doctors, and senior medical students in Bangladesh from October 2019 to February 2020. A pre-tested structured questionnaire (containing twelve close-ended questions) was used. The perceptions of health risks due to sun exposure, and basic knowledge of the physiological and epidemiological aspects of vitamin D deficiency were tested. Results: An overall negative attitude towards sunlight in the context of Bangladesh was highlighted – 68% participants thought regular sun exposu...

PLOS ONE, 2021

Prevalence of toxigenic Vibrio cholerae O1 in aquatic reservoirs in Bangladesh apparently increas... more Prevalence of toxigenic Vibrio cholerae O1 in aquatic reservoirs in Bangladesh apparently increases coinciding with the occurrence of seasonal cholera epidemics. In between epidemics, these bacteria persist in water mostly as dormant cells, known as viable but non-culturable cells (VBNC), or conditionally viable environmental cells (CVEC), that fail to grow in routine culture. CVEC resuscitate to active cells when enriched in culture medium supplemented with quorum sensing autoinducers CAI-1 or AI-2 which are signal molecules that regulate gene expression dependent on cell density. V. cholerae O1 mutant strains with inactivated cqsS gene encoding the CAI-1 receptor has been shown to overproduce AI-2 that enhance CVEC resuscitation in water samples. Since V. cholerae non-O1 non-O139 (non-cholera-vibrios) are abundant in aquatic ecosystems, we identified and characterized naturally occurring variant strains of V. cholerae non-O1 non-O139 which overproduce AI-2, and monitored their co-...

International Journal of Bioassays, 2015

Nanotechnology exhibits a tremendous potential in agriculture and its allied fields like, aquacul... more Nanotechnology exhibits a tremendous potential in agriculture and its allied fields like, aquaculture and fisheries. It is the new tools for aquaculture, fish biotechnology, fish genetics, fish reproduction and aquatic health etc. Nanotechnology tools like nanomaterials, nanosensors, DNA nanovaccines, Gene delivery, Drug delivery, Fluorescent biological labels, Probing of DNA structure, Bio detection of pathogens, Separation and purification of biological molecules and cells, antioxidant activity, antimicrobial activity cell toxicity etc. have the potential role to solve many problems like animal health, production, reproduction, prevention and treatment of diseases. It may presume that in the recent years, nanotechnology research will reform the science and technology and will help to boost up the livestock production in future. Nanotechnology applications in the fish processing industry can also be utilized to detect fish bacteria in packaging, produce stronger flavors, colour qua...

Mymensingh medical journal : MMJ, 2021

Danis-Weber type-B ankle fracture is one of the most common injuries in young and active individu... more Danis-Weber type-B ankle fracture is one of the most common injuries in young and active individual. This fracture offers a considerable challenge to orthopedic surgeon. Though there are several options for treating such type of fracture, ORIF by Pre-contoured Distal Fibular Locking Plate is the preferred option in the recent years. This descriptive type of observational study was performed from July 2017 to June 2019 in NITOR. Thirty (30) patients, 22 male and 8 female with an average age of 39 years with Danis-Weber type-B ankle fracture underwent ORIF by Pre-contoured Distal Fibular Locking Plate for fractured fibula and ORIF by 4.0mm cannulated cancellous screw for fractured medial malleolus. All the patients were initially managed by analgesic and short leg posterior slab. Average follow up was 24 weeks. Final outcome was assessed by AOFAS score. The main cause of injury was RTA (56.67%). Mean operation time was 1.2 hours. Mean duration of Hospital stay was 16.43±1.73 days. Sup...

BackgroundSouth Asian countries including Bangladesh have been struggling to control the COVID-19... more BackgroundSouth Asian countries including Bangladesh have been struggling to control the COVID-19 pandemic despite imposing months of lockdown and other public health measures (as of June 30, 2020). In-depth epidemiological information from these countries is lacking. From the perspective of Bangladesh, this study aims to understand the epidemiological features and gaps in public health preparedness.MethodThis study used publicly available data (8 March-30 June 2020) from the respective health departments of Bangladesh and Johns Hopkins University Coronavirus Resource Centre. Descriptive statistics was used to report the incidence, case fatality rates (CFR), and trend analysis. Spatial distribution maps were created using ArcGIS Desktop. Infection dynamics were analyzed via SIR models.FindingsIn 66 days of nationwide lockdown and other public health efforts, a total of 47,153 cases and 650 deaths were reported. However, the incidence was increased by around 50% within a week after r...

Md. Mahbub Hasan University of Chittagong Khaza Md. Kapil Uddin University of Chittagong Syed Moh... more Md. Mahbub Hasan University of Chittagong Khaza Md. Kapil Uddin University of Chittagong Syed Mohammad Lokman University of Chittagong Adnan Mannan University of Chittagong Enayetur Raheem Biomedical Research Foundation, Bangladesh Shahed Ahmad Chowdhury Chittagong Medical College Mohammad Sorowar Hossain (  sorowar.hossain@brfbd.org ) Biomedical Research Foundation https://orcid.org/0000-0001-7143-2909

Orphanet Journal of Rare Diseases, 2021

Background Thalassaemia, a hereditary haemoglobin disorder, is a major public health concern in s... more Background Thalassaemia, a hereditary haemoglobin disorder, is a major public health concern in some parts of the world. Although Bangladesh is in the world’s thalassaemia belt, the information on this disease is scarce. Additionally, the awareness of this life threatening, but potentially preventable disease is surprisingly poor. However, mass awareness is pivotal for the development of an effective preventive strategy. In this context, the understanding of parental perspectives is essential to grasp the magnitude of the problem. Therefore, this study aimed to investigate the parental knowledge gaps and perceptions regarding thalassemia, the barriers confronted by the parents for caring for their thalassaemic children and their attitude to prenatal screening and prenatal diagnosis. Methods This cross-sectional study was conducted between January 2018 and December 2018 at a dedicated thalassemia hospital located in Dhaka. A structured questionnaire was used for face-to-face intervie...

BackgroundSouth Asian countries including Bangladesh have been struggling to control the COVID-19... more BackgroundSouth Asian countries including Bangladesh have been struggling to control the COVID-19 pandemic despite imposing months of lockdown and other public health measures. In-depth epidemiological information from these countries is lacking. From the perspective of Bangladesh, this study aims at understanding the epidemiological features and gaps in public health preparedness and risk communication. MethodsThe study used publicly available data of seven months (8 March 2020–10 September 2020) from the respective health departments of Bangladesh and Johns Hopkins University Coronavirus Resource Centre. Human mobility data were obtained from Google COVID-19 Community Mobility Reports. Spatial distribution maps were created using ArcGIS Desktop. Descriptive statistics was used to report the incidence, case fatality rates (CFR), and trend analysis. Results Despite nationwide lockdown, an increase in human mobility linked to specific public events was observed. During this period, a...

Corona Virus Disease-2019 (COVID-19) warrants comprehensive investigations of publicly available ... more Corona Virus Disease-2019 (COVID-19) warrants comprehensive investigations of publicly available Severe Acute Respiratory Syndrome-CoronaVirus-2 (SARS-CoV-2) genomes to gain new insight about their epidemiology, mutations and pathogenesis. Nearly 0.4 million mutations were identified so far in ∼60,000 SARS-CoV-2 genomic sequences. In this study, we compared 207 of SARS-CoV-2 genomes reported from different parts of Bangladesh and their comparison with 467 globally reported sequences to understand the origin of viruses, possible patterns of mutations, availability of unique mutations, and their apparent impact on pathogenicity of the virus in victims of Bangladeshi population. Phylogenetic analyses indicates that in Bangladesh, SARS-CoV-2 viruses might arrived through infected travelers from European countries, and the GR clade was found as predominant in this region. We found 2602 mutations including 1602 missense mutations, 612 synonymous mutations, 36 insertions and deletions with...

Malaysian Journal on Composites Science and Manufacturing, 2020

Nowadays, NR/EPDM have drawn great attention to most researchers to be utilized in an engine moun... more Nowadays, NR/EPDM have drawn great attention to most researchers to be utilized in an engine mounting. In service, majority of structures and components are subjected to service condition such as heat, liquids and stress of dynamic loading. This research is an effort to compare the thermal effects on physical and mechanical properties of NR/EPDM blend and NR/EPDM filled graphene nanoplatelets (GNPs) for the potential usage in the engine mounting. The blends and nanocomposites were prepared through a melt compounding using a Banbury Internal Mixer, exposed to thermal cycles and tensile tested. The materials were heated alternately for 10 minutes at 60℃ and 120℃ for 0, 35, 70 and 150 cycles. Thermally aged NR/EPDM nanocomposites showed higher performance under tensile stress if compared to NR/EPDM blend. The tensile strength and elastic modulus were consistently 40% higher than the strength of the blend. This is due to the reinforcing effects of GNPs and their good interaction with the matrix. The reduction of tensile properties was due to the embrittlement effect and in line with the increase in amorphous phases from the broadening of XRD spectra. This has proven that the NR/EPDM nanocomposites can withstand thermal cycles, oils and fluctuating stress better than NR/EPDM blend and exhibited higher potential to be utilized as the engine mount material.

Journal of Food Science and Engineering, 2012

Functional properties of a food depends on its capacity to act as a natural antioxidant, by scave... more Functional properties of a food depends on its capacity to act as a natural antioxidant, by scavenging lipid peroxides and also by the ability of the particular food component to bind fat and sugars efficiently. This study was undertaken to primarily screening out the functional properties of different Bangladeshi food. In this study, the antioxidant properties, total phenolic content, flavonoid and lipid peroxide contents of eight commonly used fruits and vegetables were examined. The ethanol extract of fruits including Amra (Hog Plum-Spondias dulcis), Jambura (Grape Fruit-Citrus maxima), Anaros (Pineapple-Ananas comosus), and Kamranga (Carambola-Averrhoa carambola), and the vegetables including Kolmi Shak (Water spinach-Ipomoea aquatica), Lal Shak (Red amaranth-Amaranthus gangeticus), Data Shak (Stem amaranth-Amaranthus lividus), Korola (Gourd-Momordica charantia) were examined. Both the fat and sugar binding capacity was found the highest in Anaros and lowest in Amra. Mortality rate of Brine shrimp (Artemia salina) nauplii in brine shrimp lethality bioassay at different concentration of fruits and vegetables extract showed that Jambura has the highest cytotoxic effect, killing 100% of the brine shrimp at a very low concentration (0.01 mg/mL), compared to the effect of positive control (0.5 mg/mL of the Vincristine sulfate). In contrast, Lalshak showed the lowest (0% and 30% mortality) in vitro cytotoxic effect at both 0.01 and 0.1 mg/mL concentration. The Lal shak and Anaros extracts significantly reduced lipid peroxide (LPO) levels in the liver tissue homogenates. However, regarding the flavonoids content and phenolic content, Kamranga and Lal shak extracts showed the highest flavonoid contents, with mean values of 41.36 and 41.26 mg/g, respectively. Anaros and Jambura showed the lowest flavonoid contents, with mean values of 0.5 and 9.3 mg/g respectively. The phenolic contents as expressed as gallic acid equivalent (GAE) mg/g extracts showed that Kolmi shak had the highest amount of phenolic compounds (59.33 ± 3.72 mg/g of GAE) and Anaros has the lowest phenolic contents (11.00 ± 1.33 mg/g of GAE). Therefore, these primary findings revealed enormous potentiality of commonly consumed Bangladeshi fruits and vegetables in health sectors.

The newly identified SARS-CoV-2 has now been reported from around 183 countries with more than a ... more The newly identified SARS-CoV-2 has now been reported from around 183 countries with more than a million confirmed human cases including more than 68000 deaths. The genomes of SARS-COV-2 strains isolated from different parts of the world are now available and the unique features of constituent genes and proteins have gotten substantial attention recently. Spike glycoprotein is widely considered as a possible target to be explored because of its role during the entry of coronaviruses into host cells. We analyzed 320 whole-genome sequences and 320 spike protein sequences of SARS-CoV-2 using multiple sequence alignment tools. In this study, 483 unique variations have been identified among the genomes including 25 non-synonymous mutations and one deletion in the spike protein of SARS-CoV-2. Among the 26 variations detected, 12 variations were located at the N-terminal domain and 6 variations at the receptor-binding domain (RBD) which might alter the interaction with receptor molecules. ...

Orphanet Journal of Rare Diseases, 2020

Background Thalassaemia is a potentially life-threatening yet preventable inherited hemoglobin di... more Background Thalassaemia is a potentially life-threatening yet preventable inherited hemoglobin disorder. Understanding local socio-cultural context and level of public awareness about thalassaemia is pivotal for selecting effective prevention strategies. This study attempted to assess knowledge and perceptions about thalassaemia among college students in Bangladesh. Methods A supervised cross-sectional survey was conducted on 1578 college students using a self-administered structured questionnaire. The survey took place from 15 February 2018 to 17 March 2018 in the Jamalpur district in Bangladesh. Besides the attitude-related questions, the study asked a total of 12 knowledge-related questions, which were scored on a scale of 0–12 points. Results Over two-thirds (67%) of the college students had never heard of thalassaemia. The urban-rural dichotomy was observed among those familiar with the term; (46.4% from urban vs. 25.8% from rural colleges). A similar pattern was observed for k...

Open Journal of Medical Microbiology, 2018

Enterobacteriaceae are important human pathogens that cause many food and waterborne illness. Rap... more Enterobacteriaceae are important human pathogens that cause many food and waterborne illness. Rapid emergence of multi-drug resistant bacteria in Bangladesh has become a serious problem. Phage-host interaction is now considered as the major driving force for the conversion of non-pathogenic bacteria to pathogenic ones. Evolution of highly pathogenic and antibiotic resistant bacteria largely depends upon the horizontal gene transfer by means of plasmid, megaplasmid and bacteriophages. Conversely, bacteria may acquire a novel defence mechanism called CRISPR (Clustered regularly interspersed short palindromic repeats) that can restrict horizontal transfer of plasmids and bacteriophages to limit the spread of antibiotic resistance genes among bacterial species. In this study, twenty bacterial strains were isolated from water of different medical waste and Buriganga river. Therefore, CRISPR locus was investigated following various biochemical and molecular analysis of those bacterial isolates. Identification of the bacterial isolates was conducted by Polymerase Chain Reaction (PCR) based assay of 16S rDNA extracted from those isolated strains. Results indicated that most strains were identified as Proteus mirabilis and Citrobacter freundii which mainly cause septicemia, and pneumonia in human. Thereafter, antibiogram of these strains was performed by using 11 different antibiotic discs where bacterial isolates from medical drainage system showed more resistant to antibiotics than the river

ABSTRACTMajor intrinsic proteins (MIPs), commonly known as aquaporins, facilitate the membrane di... more ABSTRACTMajor intrinsic proteins (MIPs), commonly known as aquaporins, facilitate the membrane diffusion of water and some other non- polar solutes. MIPs might be involved in host-pathogen interactions. Herein, we identified 17, 24, 27, 19, 19, and 22 full-length MIPs, respectively, in the genomes of six Phytophthora species, P. infestans, P. parasitica, P. sojae, P. ramorum, P. capsici, and P. cinnamomi. These Phytophthora species are devastating plant pathogens and members of oomycetes, a distinct lineage of fungus-like eukaryotic microbes. Phylogenetic analysis showed that the Phytophthora MIPs (PMIPs) formed a completely distinct clade from their counterparts in other taxa and were clustered into nine subgroups. Sequence and structural properties indicated that the primary selectivity-related constrictions, including aromatic arginine (ar/R) selectivity filter and Froger’s positions in PMIPs were distinct from those in other taxa. The substitutions in the conserved Asn-Pro-Ala m...

The American Journal of Tropical Medicine and Hygiene, 2019

Chikungunya (CHIK) has emerged as a major public health concern worldwide. Recently, atypical man... more Chikungunya (CHIK) has emerged as a major public health concern worldwide. Recently, atypical manifestations are drawing special attention because these might be associated with various complications. Information on atypical manifestations of CHIK is still limited. Here, we analyzed a dataset of 1,326 cases from our recent Dhaka outbreak study to explore the demographics and distributions of atypical manifestations. About 80% of cases reported at least one atypical symptom. Among all atypical symptoms, the most common and unique atypical symptom was joint pain before fever (90.2%), occurred predominantly in female respondents. Other common symptoms included red eye (68.2%), oral ulcer (37.7%), and dermatological manifestations (27.1%). More than two-thirds of patients reported multiple atypical symptoms. Atypical manifestations were not significantly different across age groups, except ocular complications. This study would be an important resource for clinicians and epidemiologists...

PloS one, 2018

Type 2 diabetes mellitus is a multifactorial metabolic disorder caused by environmental factors a... more Type 2 diabetes mellitus is a multifactorial metabolic disorder caused by environmental factors and has a strong association with hereditary issues. These hereditary issues result in an imbalance in CD4+T cells and a decreased level of naïve CD4+T cells, which may be critical in the pathogenesis of type 2 diabetes. Transcription factors GATA3 and STAT4 mediate the cytokine-induced development of naïve T cells into Th1 or Th2 types. In the present study, genetic analyses of GATA3 SNP rs3824662 and STAT4 SNP rs10181656 were performed to investigate the association of allelic and genotypic variations with the risk of T2D in the Bangladeshi population. A total of 297 unrelated Bangladeshi patients with type 2 diabetes and 247 healthy individuals were included in the study. The allelic and genotypic frequencies of rs10181656 located in the STAT4 gene were not found to be associated with risk of type 2 diabetes. The GATA3 rs3824662 T allele and mutant TT genotype had a significant associa...