Rosalía Reynoso-camacho | Universidad Autonoma de Queretaro (original) (raw)

Papers by Rosalía Reynoso-camacho

Palabras clave: Celulasas, AMPc y Regulación.

Pasta is a nutritionally unbalanced food, due to its low fat and fiber and low value of its prote... more Pasta is a nutritionally unbalanced food, due to its low fat and fiber and low value of its protein. It is considered an adequate vehicle for food supplementation with minerals, proteins and other healthy components such as bioactive compounds present in common beans. The effect of composite pasta (wheat -common bean; 30 % w/w) on the cooking quality (optimal cooking time, cooking loss, weight loss, firmness, color), total phenolic content, antioxidant capacity by DPPH and ORAC assays and phenolic acid profile was investigated. According to the quality parameters, pasta added with bean flour was less hard with respect to the pasta made from durum wheat. The total phenolic content and antioxidant capacity by DPPH and ORAC assays were higher in the pasta with common bean flour than in the pasta control. Also, more phenolic acids were identified in cooked pasta containing common bean flour as analyzed by HPLC.

Actually there is an increase on infusion consumption due their beneficial properties, which are ... more Actually there is an increase on infusion consumption due their beneficial properties, which are attributable to the presence of phenolic compounds. By this reason, the aim of this study was to assess phenolic content, antioxidant capacity and anti-inflammatory capacity of commercial infusions of peppermint (Mentha piperita L.), lemon grass (Cymbopogon citratos), chamomile (Matricaria chamomilla L.), arnica (Heterotheca inuloides) and boldo (Peumus boldus Molina) obtained in supermarket in 2009. Phenols content and antioxidant capacity were defined by spectrophotometry techniques. Also, identification and quantification of phenolic compound was made by HPLC. The anti-inflammatory activity was assessed by inhibition of ciclooxigenasa-2 (COX-2) enzyme. Boldo infusion had greatest content of phenolic compounds and greater antioxidant capacity measured by ABT techniques, followed by chamomile infusions by FRAP method. In assessed infusions ten different compounds were identified, findin...

Genes & Nutrition, 2015

Consuming a high-fat/high-fructose diet (HFD) starting at a young age leads to the development of... more Consuming a high-fat/high-fructose diet (HFD) starting at a young age leads to the development of obesity and to the progression of metabolic syndrome (MS). We are interested in the relationship between MS and DNA methylation as a mediator of the metabolic memory and the early appearance of these diseases in the progeny. To this end, Wistar rats were fed a HFD for 1 year, and every 12 weeks, biochemical analyses were performed. After 24 weeks, animals fed the HFD showed alterations related to MS such as elevated blood levels of fasting glucose, triglycerides, and insulin compared with their littermate controls. During the experimental period, the control females exhibited a 40 % lower 5-methylcytosine (5-mC) level compared to the control males. The HFD affected the 5-mC levels in males and females differently. The HFD induced a 20 % decrease in the 5-mC levels in males and a 15 % increase in females. We found that the HFD induces an early presentation of MS in the progeny of treated animals and that the DNA methylation was altered in the F 1 generation. The presentation of MS is positively associated with changes in the global percentage of 5-mC in the DNA.

Food Funct., 2015

Peppermint (Mentha piperita) infusions represent an important source of bioactive compounds with ... more Peppermint (Mentha piperita) infusions represent an important source of bioactive compounds with health benefits, which can be enhanced by applying salicylic acid (SA) during plant cultivation. The aim of this study was to evaluate the effect of SA (0, 0.5 and 2 mM) during peppermint cultivation on the chemical profile of saponins and alkaloids, as well as the anti-diabetic properties of the resulting infusions. The results showed that a 2 mM SA treatment significantly improved the chemical profiles of the infusions. Furthermore, the administration of 2 mM SA-treated peppermint infusions for 4 weeks to a high-fat diet/ streptozotocin-induced diabetic rats decreased serum glucose levels (up to 25%) and increased serum insulin levels (up to 75%) as compared with the diabetic control. This can be related to the observed protection on pancreatic β-cells. Furthermore, 0.5 and 2 mM SA-treated peppermint infusions decreased LDL (24 and 47%, respectively) and increased HDL levels (18 and 37%, respectively). In addition, all groups treated with peppermint infusions had lower serum and liver triglyceride contents, where 2 mM SA peppermint infusion showed the highest effect (44% and 56%, respectively). This is probably caused by its higher capacity to inhibit pancreatic lipase activity and lipid absorption. Moreover, SA-treated peppermint infusions improved the steatosis score in diabetic rat liver and decreased serum transaminase levels, probably as a result of the increase in steroidal saponins and alkaloids, such as trigonellin. Therefore, the application of 2 mM SA during cultivation of peppermint could be used to improve the anti-diabetic properties of peppermint infusions. † Electronic supplementary information (ESI) available. See

Food and Chemical Toxicology, 2015

Red oak (Quercus spp.) leaves are traditionally used as food in Mexico, and some of their infusio... more Red oak (Quercus spp.) leaves are traditionally used as food in Mexico, and some of their infusions have potential anticarcinogenic and anti-inflammatory effects; however, these properties have not yet been scientifically tested. The aim of this work was to explore the anti-inflammatory activity in HT-29 cells and anticarcinogenic effect in 1,2-dimethylhydrazine (DMH)-induced colon carcinogenesis of red oak infusions. Quercus infusions were prepared and administered as the sole source of drink to male Sprague-Dawley rats (1% w/v) for the entire 26-week experimental period. On week 4, rats received 8 subcutaneous injections of DMH (21 mg/kg body weight) once a week. The results showed that mean tumor (0.9 ± 0.2 vs. 2.6 ± 0.3) and multiplicity (1.2 ± 0.1 vs. 2.0 ± 0.23), and β-catenin protein level (2.2-fold) in adenocarcinomas were significantly lower in Quercus sideroxyla-treated group compared with DMH group. By contrast, Quercus durifolia and Quercus eduardii infusions had no protective effect. Additionally, the experiments in HT-29 cells confirmed that Q. sideroxyla infusion effectively decreased the levels of the inflammatory markers COX-2 and IL-8 by modulating the expression of NF-κB. These results highlight some of the molecular mechanisms related to the chemopreventive effect of Q. sideroxyla infusion and its potential value as a source of bioactive compounds.

Plant Foods for Human Nutrition, 2015

Mexico has the highest per capita consumption of corn in the world, which is consumed mainly as t... more Mexico has the highest per capita consumption of corn in the world, which is consumed mainly as tortilla. However, only a few in vivo studies have demonstrated the anticarcinogenic potential of some maize components against colon cancer, but not as a whole food product. Therefore, our objective was to evaluate the protective effect of corn tortillas against the development of colon cancer. First, blue, red, yellow and white corn grains were lime-cooked and processed to elaborate tortillas. Then, tortillas were administered into the diet (27 % w/w) to male Sprague-Dawley rats induced with the colon carcinogen 1,2-dimethylhydrazine (DMH). Our results indicated that consumption of tortillas, particularly from white and blue corns, significantly decreased adenocarcinoma incidence (up to 77.5 %) and mean number compared to DMH-treated animals. In addition, an inhibition of βglucuronidase activity, and induction of detoxifying enzymes in liver and colon, as well as a decrease in the expression of the two most important proliferative proteins (K-ras and β-catenin) involved in colon carcinogenesis, were also observed. These results highlight some of the molecular mechanisms related to the chemopreventive effect of tortillas, thus indicating that corn products retain their biological properties even after nixtamalization and tortilla processing.

Food Research International, 2015

Common bean (Phaseolus vulgaris L.) is an important source of phytochemicals with hypolipidemic p... more Common bean (Phaseolus vulgaris L.) is an important source of phytochemicals with hypolipidemic properties. Since hyperlipidemia is involved in the development of several chronic diseases, the lipid-lowering feature becomes an attractive property. This review focuses in research that has been conducted in vivo and in vitro experiments about specific bioactive compounds and the proposed mechanisms of action on serum lipids reduction. The hypocholesterolemic effect of beans has been associated with their dietary fiber and resistant starch content. The mechanism of action includes inhibition of intestinal lipid absorption, binding of bile acids, increase of fecal cholesterol excretion and a putative effect on hepatic low-density lipoproteins receptor for improved lipoproteins clearance. Short-chain fatty acids, produced by the fermentation of bean fiber and resistant starch, along with phytohemagglutinin have the ability to regulate appetite and satiety, activating gut hormones receptors and modulating orexigenic neuropeptides such as ghrelin, and anorexigenic neuropeptides such as glucagonlike peptide-1, peptide tyrosine-tyrosine and cholecystokinin. Other phytochemicals such as phytosterols and saponins reduce absorption of lipids at intestinal level by binding of bile acids, cholesterol micelles disruption and downregulation of lipogenic proteins via the liver X receptor pathway.

Journal of Agricultural and Food Chemistry, 2014

Peppermint (Mentha piperita) infusions represent an important source of antioxidants, which can b... more Peppermint (Mentha piperita) infusions represent an important source of antioxidants, which can be enhanced by inducing abiotic stress in plants. The aim of this study was to evaluate the effect of drought stress on peppermint cultivation as well as the metabolite profile, antioxidant capacity, and inhibition of digestive enzymes of resulting infusions. At 45 days after planting, irrigation was suppressed until 85 (control), 65, 35, 24, and 12% soil moisture (SM) was reached. The results showed that 35, 24, and 12% SM decreased fresh (20%) and dry (5%) weight. The 35 and 24% SM treatments significantly increased total phenolic and flavonoid contents as well as antioxidant capacity. Coumaric acid, quercetin, luteolin, and naringenin were detected only in some drought treatments; however, in these infusions, fewer amino acids and unsaturated fatty acids were identified. The 24 and 12% SM treatments slightly improved inhibition of pancreatic lipase and α-amylase activity. Therefore, induction of moderate water stress in peppermint is recommended to enhance its biological properties.

Journal of Medicinal Food, 2015

Pasupuleti/Nutraceuticals, Glycemic Health and Type 2 Diabetes, 2008

... Mexican Plants Rosalia Reynoso-Camacho, PhD, and Elvira González de Mejıa, PhD Overview ... T... more ... Mexican Plants Rosalia Reynoso-Camacho, PhD, and Elvira González de Mejıa, PhD Overview ... The immature fruit is similar to a small spherical pumpkin, with 810 cm in diameter. The mature fruit has a thin green shell, with white spots or strips (Acosta-Patino et al. 2001). ...

Plant Foods for Human Nutrition, 2005

This study compared the levels of antinutritional components and cytotoxic effect of extracts, fr... more This study compared the levels of antinutritional components and cytotoxic effect of extracts, from tepary (Phaseolus acutifolius) and common (Phaseolus vulgaris) beans. Antinutritional factors were evaluated by determining their effect on the viability of epithelial cells isolated from rat small intestine. The protein and carbohydrates content were similar in all the genotypes studied (20 and 60%, respectively). Common beans presented higher content of trypsin inhibitors, tannins and lectins than tepary beans. There was not a significant correlation between tannins and cooking time. However, water absorption and cooking time correlated significantly (p &amp;amp;amp;amp;amp;lt; 0.05). Considerable variation was observed in lectin activity (1302-18161 Ul/mg) of extracts from different beans. Tannins, lectins, trypsin inhibitors and fat content differed between bean varieties whereas protein content was similar. The percent cellularity on rat epithelial cells was significantly different among protein extracts from different bean cultivars and ranged between 53.5% and 87.4% (p &amp;amp;amp;amp;amp;lt; 0.05). These results suggest that the incorporation of tepary beans in the diet would not alter the current nutritional contribution of common beans or introduce adverse toxic effects. The agronomic characteristics of tepary beans make them attractive for cultivation. However, the harder to cook phenomenon may be a limiting factor that needs further consideration.

Cereal Chemistry Journal, 2014

Cereal Chem. 92(2): [185][186][187][188][189][190][191][192] The objective of this work was to st... more Cereal Chem. 92(2): [185][186][187][188][189][190][191][192] The objective of this work was to study the formation of resistant starch (RS) in tortillas from an ecological nixtamalization process compared with the traditional nixtamalization process. The RS increased through all the steps of tortilla production. It was found that the increase of the RS corresponds mainly to the formation of RS5 (V-amylose-lipid complex), but in tortillas two major types of RS coexist: RS5 and RS3 (retrograded starch). In general, tortillas from the ecological nixtamalization process gave higher values of protein, lipids, total dietary fiber, insoluble fiber, soluble fiber, and RS compared with tortillas from the traditional nixtamalization process and commercial flour. The highest glycemic index (GI) occurred in the tortillas from commercial flour, whereas tortillas from 0.4% CaCO 3 and 0.6% CaSO 4 were classified as medium-GI (GI 50-70). Tortillas from 0.6% CaCl 2 had the lowest value of GI. The ecological nixtamalization processes caused significant differences in quality and nutritional properties of tortillas.

Plant Foods for Human Nutrition, 2012

Nixtamalization process is the first step to obtain maize based products, like tortillas; however... more Nixtamalization process is the first step to obtain maize based products, like tortillas; however, in both the traditional and commercial processes, white grain is generally preferred. Creole maize races, mainly pigmented varieties, have increasingly attention since these are rich in anthocyanins and carotenoids. The aim of this investigation was to evaluate the antioxidant and antimutagenic activity of rich anthocyanins and carotenoids extracts from creole maize races before (grain) and after (masa and tortilla) the nixtamalization process. Most anthocyanins and carotenoids were lost during nixtamalization. Before nixtamalization, blue and red genotypes contained either higher antioxidant capacity and anthocyanin contents (963 ± 10.0 and 212.36±0.36 mg of cyanidin-3-glucoside eq/100 g, respectively) than the white and yellow genotypes. However, the highest carotenoid levels were displayed by red grains (1.01±0.07 to 1.14±0.08 μg of β-carotene eq/g extract). Anthocyanins losses were observed when the blue grains were processed into masa (83 %) and tortillas (64 %). Anthocyanins content correlated with

Food Chemistry, 2015

Plant infusions are consumed due to their beneficial effects on health, which is attributed to th... more Plant infusions are consumed due to their beneficial effects on health, which is attributed to their bioactive compounds content. However, these compounds are susceptible to degradation during processing and storage. The objective of this research was to evaluate the effect of stevia and citric acid on the stability of phenolic compounds, antioxidant capacity and carbohydrate-hydrolysing enzyme inhibitory activity of roselle beverages during storage. The optimum extraction conditions of roselle polyphenolic compounds was of 95°C/60 min, which was obtained by a second order experimental design. The incorporation of stevia increased the stability of colour and some polyphenols, such as quercetin, gallic acid and rosmarinic acid, during storage. In addition, stevia decreased the loss of ABTS, DPPH scavenging activity and a-amylase inhibitory capacity, whereas the incorporation of citric acid showed no effect.

Pinto Zapata redujo 36% la incidencia, número y volumen de tumores (1 y 0.043 cm 3 , respectivame... more Pinto Zapata redujo 36% la incidencia, número y volumen de tumores (1 y 0.043 cm 3 , respectivamente) comparado con el número (2 a 4) y volumen de tumores (0.055 a 0.95 cm 3 ) en ratas alimentadas con las otras variedades. A pesar de las diferencias en la composición química de las variedades de frijol, no se determinó relación alguna entre los compuestos estudiados y los resultados de incidencia del cáncer de colon. Los resultados mostraron que el consumo del grano redujo la incidencia de tumores cancerígenos en ratas Sprague-Dawley.

Plant Foods for Human Nutrition, 2007

Jamapa bean is a black Phaseolus vulgaris variety rich in condensed tannins, anthocyanins and fla... more Jamapa bean is a black Phaseolus vulgaris variety rich in condensed tannins, anthocyanins and flavonols with interesting biological activities. The objective of this work was to evaluate the antiradical capacity (ARC) of a Jamapa bean methanolic extract (BME) and some of the proanthocyanidin-rich fractions derived from it, using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The effect of the BME on some proteins involved in apoptosis on HeLa cells was also evaluated. A strong correlation between proanthocyanidin concentration in BME and antiradical capacity was found, suggesting that these compounds contribute significantly to antiradical activity. BME was a better radical scavenger than butylated hydroxytoluene (45.6 and 33.9% ARC at 400 µM, respec-tively). Two proanthocyanidin-rich fractions obtained after a preliminary separation of the BME using Toyopearl (TP4 and TP6) exhibited a higher antiradical activity than the parent extract. The treatment of HeLa cells with 35 µg BME/ml/24 h increased the expression of Bax and Caspase-3, pro-apoptotic proteins (6.13 and 1.2 times for Caspase-3 and Bax, respectively). The mechanism of action of some proteins involved in apoptosis was also evaluated, and the results suggest that black Jamapa bean could be an important source of polyphenolic compounds with potential biological use as antioxidant and anticancer agents.

$Research paper thumbnail of Non-digestible fraction of beans (Phaseolus vulgaris L.) modulates signalling pathway genes at an early stage of colon cancer in Sprague–Dawley rats$

British Journal of Nutrition, 2012

Colorectal cancer is one of the most common causes of morbidity and mortality in Western countrie... more Colorectal cancer is one of the most common causes of morbidity and mortality in Western countries, the second cause of cancer mortality in the USA and a major public health problem in Mexico. A diet rich in legumes is directly related to the prevention of colon cancer, showing an inverse relationship with the development of colorectal adenomas in human subjects. The present study shows the results of molecular changes involved in the Tp53 pathway at an early stage in the distal colon tissue of azoxymethane (AOM)-induced colon cancer in rats evaluated by PCR array after exposure to diets containing the non-digestible fraction (NDF) of cooked bean (cultivar Bayo Madero). Significant differences were detected in seventy-two genes of the Tp53-mediated signalling pathway involved in apoptosis, cell-cycle regulation and arrest, inhibition of proliferation and inflammation, and DNA repair. Tp53, Gadd45a, Cdkn1a and Bax were highly expressed (9·3-, 18·3-, 5·5-and 3·5-fold, respectively) in the NDF þ AOM group, whereas Cdc25c, Ccne2, E2f1 and Bcl2 were significantly suppressed (29·2-, 22·6-, 218·4and 2 3·5-fold, respectively), among other genes, compared with the AOM group, suggesting that chemoprevention of aberrant crypt foci results from a combination of cell-cycle arrest in G1/S and G2/M phases and cell death by apoptotic induction. We demonstrate that the NDF from common bean modulates gene expression profiles in the colon tissue of AOM-induced rats, contributing to the chemoprotective effect of common bean on early-stage colon cancer.