Antonio Celada | Universitat de Barcelona (original) (raw)
Papers by Antonio Celada
Clinical microbiology, 2017
Osteomyelitis is a bone infection characterized by progressive inflammatory destruction of the in... more Osteomyelitis is a bone infection characterized by progressive inflammatory destruction of the infected bone and new apposition of bone at the site of infection. In adults, osteomyelitis is usually a complication of open wounds due to fractures, surgery, or both, with or without the presence of foreign bodies such as prosthetic devices. In fact, it is estimated that about 0.4 to 7% of trauma and orthopaedic interventions are complicated by osteomyelitis [1-6].
Journal of Investigative Dermatology, Jul 1, 2020
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Frontiers in Immunology, Jun 27, 2018
Streptococcus pyogenes throat infection is a clinically relevant trigger of both guttate and chro... more Streptococcus pyogenes throat infection is a clinically relevant trigger of both guttate and chronic plaque psoriasis, and it provides an ideal context in which to study the pathogenesis of these diseases using an antigen-dependent approach. Circulating cutaneous lymphocyte-associated antigen (CLA) positive (+) memory T cells are a subset of peripheral lymphocytes whose phenotype and function are related to immunological mechanisms in the skin. These cells are considered peripheral biomarkers of T-cell-mediated skin diseases. The coculture of autologous epidermal cells with CLA + T cells from psoriasis patients activated by S. pyogenes allows the reproduction of the ex vivo initial molecular events that occur during psoriatic lesion formation. With cooperation of autologous epidermal cells, S. pyogenes selectively activates CLA + T cells both in guttate and plaque psoriasis, inducing key mediators, including an IL-17 response. Here, we explore potential new mechanisms of psoriasis development including the influence of HLA-Cw6 on S. pyogenes CLA + T cell activation in guttate psoriasis, the relevance of IL-9 on microbe induced IL-17 response in guttate and plaque psoriasis, and novel effector functions of Candida albicans. This review will summarize recent knowledge of psoriatic mechanisms elicited by microbes that have been studied through an innovative translational perspective based on CLA + T cell-mediated cutaneous immune response.
Tissue Antigens, Dec 11, 2008
The distribution of HLA antigens was studied in 40 patients suffering from systemic lupus erythem... more The distribution of HLA antigens was studied in 40 patients suffering from systemic lupus erythematosus. For loci A and B, increased frequencies of Al, B8 and the presence of only one B antigen were found. Nevertheless, in relation to the number of antigens tested, this increase was not significant. For the DRw locus, DRw3 was significantly increased (P > 0.001, after correction for the 39 antigens tested). However, the patients with DRw3 did not show any correlation with a specific clinical picture or the presence in their serum of lymphocytotoxic antibodies or autoantibodies against T and B lymphocytes.
Journal of Parasitology, Feb 1, 1983
Polymorphonuclear leukocytes (PMN) from normal blood donors phagocytosed P. falciparum-infected r... more Polymorphonuclear leukocytes (PMN) from normal blood donors phagocytosed P. falciparum-infected red blood cells (IRBC) to a greater extent than normal RBC under in vitro culture condition. The phagocytic activity of PMN was greatly increased by the addition of sera from individuals living in areas endemic for malaria (immune sera) but not by sera from individuals recovering from a first acute P. falciparum infection. The enhancement of the phagocytic activity was associated with the purified IgG fraction of immune sera and was lost after absorption of IgG on protein A sepharose column of preincubation of the immune sera with IRBC. These experiments suggest that opsonisation of IRBC and their subsequent phagocytosis may be one of the mechanisms involved in the clearance of P. falciparum infection in individuals living in areas endemic for malaria.
Springer eBooks, Aug 24, 2016
Monocytes and macrophages are crucial effectors and regulators of the immune system. It has recen... more Monocytes and macrophages are crucial effectors and regulators of the immune system. It has recently been shown that most tissue macrophages are produced locally, including microglia and Langerhans cells, among others (Geissmann et al. Science 327:656–661, 2010). Monocytes that circulate in the bloodstream are produced in the bone marrow and can be classified into two types. The first type has a surveillance function and is recognized by the cluster of differentiation (CD) surface markers, namely CD14+CD16++ in humans and Ly6 (lymphocyte antigen 6) Clow in mice, while the second type comprises effector cells that are involved in the inflammatory process and are recognized by CD14++CD16− in humans and Ly6Chigh in mice (Geissmann et al. Science 327:656–661, 2010). Monocytes perform immune surveillance by continuously checking the surrounding environment for signs of damage or infection. Once inside tissues, monocytes differentiate and become macrophages. In addition to eliminating pathogens, macrophages also perform other functions. They contribute to the clearance of dust and allergens in lungs (alveolar macrophages) and clear toxins from the liver (Kupffer cells) and senescent red blood cells from the spleen (splenic macrophage), and they induce immune tolerance in intestine (intestinal macrophage) (Murray and Wynn. Nat Rev Immunol 11:723–737, 2011). Belonging to the myeloid lineage, macrophages have diverse functions, including the initiation and resolution of inflammation, waste disposal, angiogenesis, bone remodeling, and the regulation of lipid metabolism, iron metabolism, and wound healing. Impairment of proper macrophage function leads to an imbalance in the immune response and, in extreme cases, to disease. These immune cells are characterized by the expression of specific surface markers, among these, CD11b, EGF-like (epidermal growth factor-like) module (Emr1 or F4/80), CD68, colony-stimulating factor 1 receptor (CSF1r), lymphocyte antigen 6 (Ly6)C, and Ly6G (Murray and Wynn. Nat Rev Immunol 11:723–737, 2011).
Transfusion, 1984
Circulating immune complexes were examined in patients with hemophilia or von Willebrand&... more Circulating immune complexes were examined in patients with hemophilia or von Willebrand's disease in order to determine the immediate or long-term side effects after transfusion. The conglutinin binding assay which allows quantitation of C3bi-bearing immune complexes was used for 82 patients with hemophilia A. Immune complexes were detected in 37 (45%) of these cases prior to transfusion. Immune complexes also were detected in four of 11 patients with hemophilia A and factor VIII inhibitors, in five of 11 patients with hemophilia B, and in three of 10 patients with von Willebrand's disease. The levels of circulating immune complexes in 21 patients with hemophilia A and seven with von Willebrand's disease significantly increased 24 hours after concentrate or cryoprecipitate transfusions. Purified immune complexes from three patients with hemophilia A were shown to contain IgG, IgM, and complement components. No factor VIII coagulant or antigenic protein or fibrinogen was identified in the immune complexes using specific antisera. Side effects immediately after transfusion were not associated with immune complexes. The levels of factor VIII or IX after transfusion were not particularly decreased in relation to the presence of immune complexes. Finally, the presence of circulating immune complexes in the patients studied did not correlate with the number of transfusions, the units of concentrates injected, the presence of HBsAg or HbsAb, the levels of plasma aspartate transferase, or the presence of rheumatoid factor. Proteinuria was absent in all the patients studied.
International Archives of Allergy and Immunology, 1984
Monocytes and polymorphonuclear leukocytes from normal blood donors phagocytosed preferentially P... more Monocytes and polymorphonuclear leukocytes from normal blood donors phagocytosed preferentially Plasmodium falciparum-infected red blood cells (IRBC) in presence of sera from individuals living in areas endemic for malaria. Total complement or factor B heat inactivation of immune or normal serum does not alter opsonic activity directed against IRBC.
![Research paper thumbnail of Circulating immune complexes in thrombotic thrombocytopenic purpura (TTP) [letter]](https://attachments.academia-assets.com/116942538/thumbnails/1.jpg)
](Blood, Oct 1, 1978
Thrombotic thrombocytopenic purpura: A Fl: The Raji cell radioimmunoassay for desyndrome of intra... more Thrombotic thrombocytopenic purpura: A Fl: The Raji cell radioimmunoassay for desyndrome of intravascular platelet consump-tecting immune complexes in human sera. I tion.
The Journal of Immunology
The mechanism of gene expression for the MHC I-A beta and TNF genes was studied in murine bone ma... more The mechanism of gene expression for the MHC I-A beta and TNF genes was studied in murine bone marrow macrophages. The treatment of macrophages with PMA stimulated the expression of TNF, but not I-A beta, suggesting that the TNF gene is responsive to activators of protein kinase C whereas the I-A beta gene is not. The treatment of macrophages with IFN-gamma led to an increase in the level of RNA for both TNF and I-A beta. The increase in expression of I-A beta and TNF, induced by IFN-gamma, was blocked by naphthalenesulfonamide or phenothiazine (trifluoperazine) but was not affected by the addition of isoquinolinesulfonamide or sphingosine. These results suggest that the induced expression of I-A beta and TNF by IFN-gamma is mediated by a pathway that is protein kinase C independent. This was supported by the finding that calcium ionophores were also able to induce the gene expression of both TNF and I-A beta. We observed that when both IFN-gamma and PMA were added to the macrophage...
Inflammation occurs when the body suffers aggression either by microbes, trauma or a variety of p... more Inflammation occurs when the body suffers aggression either by microbes, trauma or a variety of physical agents, such as heat, radiation, etc. Inflammation is also involved in the pathogenesis of chronic diseases of autoimmune origin (eg rheumatoid arthritis and diabetes) and cancer. In the early stages of inflammation, there is an increase in the size of the vessels around the inflammatory loci and the release of liquids. After, distinct cells reach these loci in a highly specific order: in the first 24 h neutrophils, at 48 h macrophages, and several days later lymphocytes. Neutrophils kill most types of microbes. In the initial stages of inflammation, macrophages eliminate the remaining microbes that escape the neutrophils, remove the apoptotic bodies of dead neutrophils and present antigen to T-lymphocytes, thereby initiating the mechanisms of acquired immunity, which ends in the production of antibodies and cytokines and memory cells, the latter a key element for the vaccines. M...
Els macrofags tenen un paper clau en la inflamacio. Durant l’inici del proces inflamatori aqueste... more Els macrofags tenen un paper clau en la inflamacio. Durant l’inici del proces inflamatori aquestes cel·lules s’activen i mostren una potent funcio fagocitica i microbicida que pot tenir efectes destructius en els teixits on actua. L’activacio dels macrofags implica la induccio de mes de quatre-cents gens, i dona com a resultat mes capacitat per eliminar els bacteris i per regular moltes altres cel·lules a traves de l’alliberament de citocines i quimiocines. L’activacio excessiva d’aquestes cel·lules te efectes perjudicials, com ara el xoc septic, que pot conduir a la sindrome de disfuncio organica multiple i a la mort. En altres situacions la persistencia dels resultats de l’activitat proinflamatoria pot contribuir al desenvolupament de processos d’inflamacio cronica, com l’artritis reumatoide, la psoriasi i la malaltia inflamatoria de l’intesti. Per evitar aquests efectes indesitjables els macrofags han desenvolupat diversos mecanismes per regular l’exces d’activacio, de manera que...
Journal of Investigative Dermatology, 2019
Psoriasis is a common skin inflammatory disorder known for its hallmark of having abnormal kerati... more Psoriasis is a common skin inflammatory disorder known for its hallmark of having abnormal keratinocyte differentiation. In psoriatic skin, the expression of liver X receptors (LXRa and LXRb), a nuclear receptor family of transcription factors has shown to be down-regulated. In addition, LXR plays an integral part in the control of keratinocyte differentiation. However, very few studies have been conducted to understand the role of LXRs during skin inflammation. We hypothesized that LXRs in the skin can interfere the immune cascade in the induction of psoriatic inflammation. We first evaluated imiquimod (IMQ)-induced psoriatic inflammation in murine ear skin that was simultaneously treated with or without an LXR agonist (GW3965) for 11 days. We found that IMQ-induced skin inflammation was milder in mice treated with GW3965 than in mice treated with its vehicle. In addition, qPCR analysis demonstrated that the gene expression levels of pro-resolution mediators (ABCA1, MerTK and TGF-b) in the IMQ-treated skin were higher in the LXR agonist-treated group than in the control group. We also performed lipidomics analysis to understand the role of the LXR agonist for the generation of lipid mediators in the skin. The levels of key immune recruitment lipid mediators (leukotriene D4 and prostaglandin E2) in the skin lesions were lower in the LXR agonist-treated group than in the control group. To further illustrate the role of LXRs for the immune cell recruitment in psoriatic skin inflammation, we performed whole mount skin imaging. We observed that neutrophil recruitment in the IMQ-treated skin were markedly lower in the LXR agonist-treated group compared to the control group. These results suggest that the activation of LXRs can alleviate psoriatic skin inflammation via the generation of proresolving molecules and the regulation of the synthesis of lipid mediators.
Journal of Leukocyte Biology, 2000
Activation of human B lymphocytes by lipopolysaccharide (LPS) or phorbol 12-myristate 13-acetate ... more Activation of human B lymphocytes by lipopolysaccharide (LPS) or phorbol 12-myristate 13-acetate (PMA) results in the differential regulation of nucleoside uptake [Soler, C., Felipe, A., Mata, J. F., Casado, F. J., Celada, A., Pastor-Anglada, M. (1998) J. Biol. Chem. 273, 26939–26945]. Because nitric oxide (NO) is involved in the modulation of the apoptotic response of B cells, the effects of NO on the regulatory responses of these transport systems to phorbol esters has been studied in Raji cells by a combination of approaches that involve arginine depletion, inhibition of nitric oxide synthase, and non-enzymatic production of NO using a donor. Human B lymphocytes express three transport systems involved in nucleoside uptake: N1 and N5, which are concentrative and Na+-dependent, and the nitrobenzylthioinosine-sensitive equilibrative system es. Raji cells do not express significant amounts of iNOS mRNA or protein; thus, NO production is presumably constitutive. The data are consiste...
European journal of immunology, Oct 1, 2017
Guidelines for the use of flow cytometry and cell sorting in immunological studies
Clinical and experimental immunology, 1982
In vitro human monocytes from normal blood donors ingest red blood cells infected with Plasmodium... more In vitro human monocytes from normal blood donors ingest red blood cells infected with Plasmodium falciparum more efficiently than normal red blood cells (NRBC). The phagocytic activity of human monocytes for infected red blood cells (IRBC) is greatly enhanced by the addition of immune sera obtained from individuals living in areas with endemic malaria. In contrast, the addition of sera obtained from individuals recovering from a first infection, or pooled normal sera, does not result in increased phagocytosis of IRBC. The phagocytosis enhancing activity of immune sera is associated with the IgG fraction and IgG depleted sera do not stimulate phagocytosis. Enhanced immune serum mediated phagocytosis occurs as a result of opsonization of IRBC. This was demonstrated by experiments in which monocytes or IRBC were preincubated with immune serum prior to the phagocytic assay. The opsonic activity could be absorbed by IRBC but not by NRBC. The opsonization of IRBC and subsequent phagocyto...
Genes & Development, 1990
BMJ, 1977
Thrombocytopenic purpura during treatment with Librax There have been no reports relating thrombo... more Thrombocytopenic purpura during treatment with Librax There have been no reports relating thrombocytopenia to the consumption of Librax-a combination of chlordiazepoxide (5 mg/tablet) and clidinium bromide (2 5 mg/tablet). We have observed a case of thrombocytopenic purpura apparently induced by this medication. Case report A 32-year-old White woman teacher, weighing 56 kg, consulted her doctor complaining of fatigue, nervousness, non-specific gastrointestinal disturbances, and family problems. A gastrointestinal transit test and routine blood tests showed no abnormalities. The patient had no other metabolic disease or a family history of adverse drug effects. Her doctor prescribed Librax (Roche) orally three tablets a day and a diet without acid fruits. After seven days on
Clinical microbiology, 2017
Osteomyelitis is a bone infection characterized by progressive inflammatory destruction of the in... more Osteomyelitis is a bone infection characterized by progressive inflammatory destruction of the infected bone and new apposition of bone at the site of infection. In adults, osteomyelitis is usually a complication of open wounds due to fractures, surgery, or both, with or without the presence of foreign bodies such as prosthetic devices. In fact, it is estimated that about 0.4 to 7% of trauma and orthopaedic interventions are complicated by osteomyelitis [1-6].
Journal of Investigative Dermatology, Jul 1, 2020
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Frontiers in Immunology, Jun 27, 2018
Streptococcus pyogenes throat infection is a clinically relevant trigger of both guttate and chro... more Streptococcus pyogenes throat infection is a clinically relevant trigger of both guttate and chronic plaque psoriasis, and it provides an ideal context in which to study the pathogenesis of these diseases using an antigen-dependent approach. Circulating cutaneous lymphocyte-associated antigen (CLA) positive (+) memory T cells are a subset of peripheral lymphocytes whose phenotype and function are related to immunological mechanisms in the skin. These cells are considered peripheral biomarkers of T-cell-mediated skin diseases. The coculture of autologous epidermal cells with CLA + T cells from psoriasis patients activated by S. pyogenes allows the reproduction of the ex vivo initial molecular events that occur during psoriatic lesion formation. With cooperation of autologous epidermal cells, S. pyogenes selectively activates CLA + T cells both in guttate and plaque psoriasis, inducing key mediators, including an IL-17 response. Here, we explore potential new mechanisms of psoriasis development including the influence of HLA-Cw6 on S. pyogenes CLA + T cell activation in guttate psoriasis, the relevance of IL-9 on microbe induced IL-17 response in guttate and plaque psoriasis, and novel effector functions of Candida albicans. This review will summarize recent knowledge of psoriatic mechanisms elicited by microbes that have been studied through an innovative translational perspective based on CLA + T cell-mediated cutaneous immune response.
Tissue Antigens, Dec 11, 2008
The distribution of HLA antigens was studied in 40 patients suffering from systemic lupus erythem... more The distribution of HLA antigens was studied in 40 patients suffering from systemic lupus erythematosus. For loci A and B, increased frequencies of Al, B8 and the presence of only one B antigen were found. Nevertheless, in relation to the number of antigens tested, this increase was not significant. For the DRw locus, DRw3 was significantly increased (P > 0.001, after correction for the 39 antigens tested). However, the patients with DRw3 did not show any correlation with a specific clinical picture or the presence in their serum of lymphocytotoxic antibodies or autoantibodies against T and B lymphocytes.
Journal of Parasitology, Feb 1, 1983
Polymorphonuclear leukocytes (PMN) from normal blood donors phagocytosed P. falciparum-infected r... more Polymorphonuclear leukocytes (PMN) from normal blood donors phagocytosed P. falciparum-infected red blood cells (IRBC) to a greater extent than normal RBC under in vitro culture condition. The phagocytic activity of PMN was greatly increased by the addition of sera from individuals living in areas endemic for malaria (immune sera) but not by sera from individuals recovering from a first acute P. falciparum infection. The enhancement of the phagocytic activity was associated with the purified IgG fraction of immune sera and was lost after absorption of IgG on protein A sepharose column of preincubation of the immune sera with IRBC. These experiments suggest that opsonisation of IRBC and their subsequent phagocytosis may be one of the mechanisms involved in the clearance of P. falciparum infection in individuals living in areas endemic for malaria.
Springer eBooks, Aug 24, 2016
Monocytes and macrophages are crucial effectors and regulators of the immune system. It has recen... more Monocytes and macrophages are crucial effectors and regulators of the immune system. It has recently been shown that most tissue macrophages are produced locally, including microglia and Langerhans cells, among others (Geissmann et al. Science 327:656–661, 2010). Monocytes that circulate in the bloodstream are produced in the bone marrow and can be classified into two types. The first type has a surveillance function and is recognized by the cluster of differentiation (CD) surface markers, namely CD14+CD16++ in humans and Ly6 (lymphocyte antigen 6) Clow in mice, while the second type comprises effector cells that are involved in the inflammatory process and are recognized by CD14++CD16− in humans and Ly6Chigh in mice (Geissmann et al. Science 327:656–661, 2010). Monocytes perform immune surveillance by continuously checking the surrounding environment for signs of damage or infection. Once inside tissues, monocytes differentiate and become macrophages. In addition to eliminating pathogens, macrophages also perform other functions. They contribute to the clearance of dust and allergens in lungs (alveolar macrophages) and clear toxins from the liver (Kupffer cells) and senescent red blood cells from the spleen (splenic macrophage), and they induce immune tolerance in intestine (intestinal macrophage) (Murray and Wynn. Nat Rev Immunol 11:723–737, 2011). Belonging to the myeloid lineage, macrophages have diverse functions, including the initiation and resolution of inflammation, waste disposal, angiogenesis, bone remodeling, and the regulation of lipid metabolism, iron metabolism, and wound healing. Impairment of proper macrophage function leads to an imbalance in the immune response and, in extreme cases, to disease. These immune cells are characterized by the expression of specific surface markers, among these, CD11b, EGF-like (epidermal growth factor-like) module (Emr1 or F4/80), CD68, colony-stimulating factor 1 receptor (CSF1r), lymphocyte antigen 6 (Ly6)C, and Ly6G (Murray and Wynn. Nat Rev Immunol 11:723–737, 2011).
Transfusion, 1984
Circulating immune complexes were examined in patients with hemophilia or von Willebrand&... more Circulating immune complexes were examined in patients with hemophilia or von Willebrand's disease in order to determine the immediate or long-term side effects after transfusion. The conglutinin binding assay which allows quantitation of C3bi-bearing immune complexes was used for 82 patients with hemophilia A. Immune complexes were detected in 37 (45%) of these cases prior to transfusion. Immune complexes also were detected in four of 11 patients with hemophilia A and factor VIII inhibitors, in five of 11 patients with hemophilia B, and in three of 10 patients with von Willebrand's disease. The levels of circulating immune complexes in 21 patients with hemophilia A and seven with von Willebrand's disease significantly increased 24 hours after concentrate or cryoprecipitate transfusions. Purified immune complexes from three patients with hemophilia A were shown to contain IgG, IgM, and complement components. No factor VIII coagulant or antigenic protein or fibrinogen was identified in the immune complexes using specific antisera. Side effects immediately after transfusion were not associated with immune complexes. The levels of factor VIII or IX after transfusion were not particularly decreased in relation to the presence of immune complexes. Finally, the presence of circulating immune complexes in the patients studied did not correlate with the number of transfusions, the units of concentrates injected, the presence of HBsAg or HbsAb, the levels of plasma aspartate transferase, or the presence of rheumatoid factor. Proteinuria was absent in all the patients studied.
International Archives of Allergy and Immunology, 1984
Monocytes and polymorphonuclear leukocytes from normal blood donors phagocytosed preferentially P... more Monocytes and polymorphonuclear leukocytes from normal blood donors phagocytosed preferentially Plasmodium falciparum-infected red blood cells (IRBC) in presence of sera from individuals living in areas endemic for malaria. Total complement or factor B heat inactivation of immune or normal serum does not alter opsonic activity directed against IRBC.
Blood, Oct 1, 1978
Thrombotic thrombocytopenic purpura: A Fl: The Raji cell radioimmunoassay for desyndrome of intra... more Thrombotic thrombocytopenic purpura: A Fl: The Raji cell radioimmunoassay for desyndrome of intravascular platelet consump-tecting immune complexes in human sera. I tion.
The Journal of Immunology
The mechanism of gene expression for the MHC I-A beta and TNF genes was studied in murine bone ma... more The mechanism of gene expression for the MHC I-A beta and TNF genes was studied in murine bone marrow macrophages. The treatment of macrophages with PMA stimulated the expression of TNF, but not I-A beta, suggesting that the TNF gene is responsive to activators of protein kinase C whereas the I-A beta gene is not. The treatment of macrophages with IFN-gamma led to an increase in the level of RNA for both TNF and I-A beta. The increase in expression of I-A beta and TNF, induced by IFN-gamma, was blocked by naphthalenesulfonamide or phenothiazine (trifluoperazine) but was not affected by the addition of isoquinolinesulfonamide or sphingosine. These results suggest that the induced expression of I-A beta and TNF by IFN-gamma is mediated by a pathway that is protein kinase C independent. This was supported by the finding that calcium ionophores were also able to induce the gene expression of both TNF and I-A beta. We observed that when both IFN-gamma and PMA were added to the macrophage...
Inflammation occurs when the body suffers aggression either by microbes, trauma or a variety of p... more Inflammation occurs when the body suffers aggression either by microbes, trauma or a variety of physical agents, such as heat, radiation, etc. Inflammation is also involved in the pathogenesis of chronic diseases of autoimmune origin (eg rheumatoid arthritis and diabetes) and cancer. In the early stages of inflammation, there is an increase in the size of the vessels around the inflammatory loci and the release of liquids. After, distinct cells reach these loci in a highly specific order: in the first 24 h neutrophils, at 48 h macrophages, and several days later lymphocytes. Neutrophils kill most types of microbes. In the initial stages of inflammation, macrophages eliminate the remaining microbes that escape the neutrophils, remove the apoptotic bodies of dead neutrophils and present antigen to T-lymphocytes, thereby initiating the mechanisms of acquired immunity, which ends in the production of antibodies and cytokines and memory cells, the latter a key element for the vaccines. M...
Els macrofags tenen un paper clau en la inflamacio. Durant l’inici del proces inflamatori aqueste... more Els macrofags tenen un paper clau en la inflamacio. Durant l’inici del proces inflamatori aquestes cel·lules s’activen i mostren una potent funcio fagocitica i microbicida que pot tenir efectes destructius en els teixits on actua. L’activacio dels macrofags implica la induccio de mes de quatre-cents gens, i dona com a resultat mes capacitat per eliminar els bacteris i per regular moltes altres cel·lules a traves de l’alliberament de citocines i quimiocines. L’activacio excessiva d’aquestes cel·lules te efectes perjudicials, com ara el xoc septic, que pot conduir a la sindrome de disfuncio organica multiple i a la mort. En altres situacions la persistencia dels resultats de l’activitat proinflamatoria pot contribuir al desenvolupament de processos d’inflamacio cronica, com l’artritis reumatoide, la psoriasi i la malaltia inflamatoria de l’intesti. Per evitar aquests efectes indesitjables els macrofags han desenvolupat diversos mecanismes per regular l’exces d’activacio, de manera que...
Journal of Investigative Dermatology, 2019
Psoriasis is a common skin inflammatory disorder known for its hallmark of having abnormal kerati... more Psoriasis is a common skin inflammatory disorder known for its hallmark of having abnormal keratinocyte differentiation. In psoriatic skin, the expression of liver X receptors (LXRa and LXRb), a nuclear receptor family of transcription factors has shown to be down-regulated. In addition, LXR plays an integral part in the control of keratinocyte differentiation. However, very few studies have been conducted to understand the role of LXRs during skin inflammation. We hypothesized that LXRs in the skin can interfere the immune cascade in the induction of psoriatic inflammation. We first evaluated imiquimod (IMQ)-induced psoriatic inflammation in murine ear skin that was simultaneously treated with or without an LXR agonist (GW3965) for 11 days. We found that IMQ-induced skin inflammation was milder in mice treated with GW3965 than in mice treated with its vehicle. In addition, qPCR analysis demonstrated that the gene expression levels of pro-resolution mediators (ABCA1, MerTK and TGF-b) in the IMQ-treated skin were higher in the LXR agonist-treated group than in the control group. We also performed lipidomics analysis to understand the role of the LXR agonist for the generation of lipid mediators in the skin. The levels of key immune recruitment lipid mediators (leukotriene D4 and prostaglandin E2) in the skin lesions were lower in the LXR agonist-treated group than in the control group. To further illustrate the role of LXRs for the immune cell recruitment in psoriatic skin inflammation, we performed whole mount skin imaging. We observed that neutrophil recruitment in the IMQ-treated skin were markedly lower in the LXR agonist-treated group compared to the control group. These results suggest that the activation of LXRs can alleviate psoriatic skin inflammation via the generation of proresolving molecules and the regulation of the synthesis of lipid mediators.
Journal of Leukocyte Biology, 2000
Activation of human B lymphocytes by lipopolysaccharide (LPS) or phorbol 12-myristate 13-acetate ... more Activation of human B lymphocytes by lipopolysaccharide (LPS) or phorbol 12-myristate 13-acetate (PMA) results in the differential regulation of nucleoside uptake [Soler, C., Felipe, A., Mata, J. F., Casado, F. J., Celada, A., Pastor-Anglada, M. (1998) J. Biol. Chem. 273, 26939–26945]. Because nitric oxide (NO) is involved in the modulation of the apoptotic response of B cells, the effects of NO on the regulatory responses of these transport systems to phorbol esters has been studied in Raji cells by a combination of approaches that involve arginine depletion, inhibition of nitric oxide synthase, and non-enzymatic production of NO using a donor. Human B lymphocytes express three transport systems involved in nucleoside uptake: N1 and N5, which are concentrative and Na+-dependent, and the nitrobenzylthioinosine-sensitive equilibrative system es. Raji cells do not express significant amounts of iNOS mRNA or protein; thus, NO production is presumably constitutive. The data are consiste...
European journal of immunology, Oct 1, 2017
Guidelines for the use of flow cytometry and cell sorting in immunological studies
Clinical and experimental immunology, 1982
In vitro human monocytes from normal blood donors ingest red blood cells infected with Plasmodium... more In vitro human monocytes from normal blood donors ingest red blood cells infected with Plasmodium falciparum more efficiently than normal red blood cells (NRBC). The phagocytic activity of human monocytes for infected red blood cells (IRBC) is greatly enhanced by the addition of immune sera obtained from individuals living in areas with endemic malaria. In contrast, the addition of sera obtained from individuals recovering from a first infection, or pooled normal sera, does not result in increased phagocytosis of IRBC. The phagocytosis enhancing activity of immune sera is associated with the IgG fraction and IgG depleted sera do not stimulate phagocytosis. Enhanced immune serum mediated phagocytosis occurs as a result of opsonization of IRBC. This was demonstrated by experiments in which monocytes or IRBC were preincubated with immune serum prior to the phagocytic assay. The opsonic activity could be absorbed by IRBC but not by NRBC. The opsonization of IRBC and subsequent phagocyto...
Genes & Development, 1990
BMJ, 1977
Thrombocytopenic purpura during treatment with Librax There have been no reports relating thrombo... more Thrombocytopenic purpura during treatment with Librax There have been no reports relating thrombocytopenia to the consumption of Librax-a combination of chlordiazepoxide (5 mg/tablet) and clidinium bromide (2 5 mg/tablet). We have observed a case of thrombocytopenic purpura apparently induced by this medication. Case report A 32-year-old White woman teacher, weighing 56 kg, consulted her doctor complaining of fatigue, nervousness, non-specific gastrointestinal disturbances, and family problems. A gastrointestinal transit test and routine blood tests showed no abnormalities. The patient had no other metabolic disease or a family history of adverse drug effects. Her doctor prescribed Librax (Roche) orally three tablets a day and a diet without acid fruits. After seven days on