Juan Carlos Troiano | Universidad de Buenos Aires (original) (raw)

Papers by Juan Carlos Troiano

Cuadernos de Herpetología, 1996

RESUMEN: Se presentan hallazgos de Trematodes en algunos ofidios provenientes de necropsias en 50... more RESUMEN: Se presentan hallazgos de Trematodes en algunos ofidios provenientes de necropsias en 50 ejemplares de diferentes generos y especies de ofidios capturados en las provincias de Corrientes, Misiones y Chaco y enviados al Servicio de Identificacion de Animales Ponzonosos y No Ponzonosos anexo a la Catedra de Zoologia y Recursos Faunicos, de la Facultad de Ciencias Veterinarias UNNE. Una vez extraidos los trematodes. se fijaron en solucion Raillet -Henry y se colorearon con la tecnica del carmin clorhidrico acetico. para su clasificacion. Se identificaron 7 especies de trematodes ya descriptos (Travtrema stenocotyle; Telorchis clava; Glossidiella °mata; Microderma luhei; Opisthogonimus lecithonothus e Infidum infidum) y nuevos hospedadores (Hydrodynastes gigas; Bothrops jararaca; Bothrops moojeni; Thammodynastes strigatus y Bothrops neuwedii diporus). siendo los lugares mas afectados los del aparato digestivo, especialmente la boca e intestino grueso. Palabras clave: Trematodes...

The finding of macroscopic and microscopic lesions in lungs of a Hydrodinastes gigas (ñacanina) f... more The finding of macroscopic and microscopic lesions in lungs of a Hydrodinastes gigas (ñacanina) from Northeastern Argentina are described. In accordance with the localization and the histopathological findings it is concluded that the case described is a nymphal infestation by pentastomid of Porocephalus (Humboldt, 1811) genus. This is the first report of Porocephalus in Hydrodinastes gigas in Argentina. Some aspects related to the final host, life cycle and potential pathogenicity for humans is discussed.

One of the methods used for the conservation of the South American caimans is ranching, a techniq... more One of the methods used for the conservation of the South American caimans is ranching, a technique for the sustainable management and conservation of the species. It includes environmental and population studies which are followed by the collection of eggs from nature after authorization by the relevant agencies, which are then incubated artificially and fattened for commercial purposes. This process may lead to alterations in the eggs that cause different degrees of congenital anomalies that affect the future life of the animal to a greater or lesser extent. Eggs collected in the natural environment may suffer unfavorable thermal variations, which in certain phases of embryonic development may lead to the appearance of a teratogen. In this work we describe the finding of a parasitic univithelial twin in Caiman yacare. The possible causes of this anomaly and a review of congenital anomalies in crocodiles are described.

Clínica Veterinária, 2017

This article reports for the first time the isolation of a Chrysosporium species from a juvenile ... more This article reports for the first time the isolation of a Chrysosporium species from a juvenile green iguana (Iguana iguana) in Argentina. Since the animal presented a lesion in the distal third of the tail and had been refractory to different treatments, caudal amputation was the elected approach. Sterile tissue samples were seeded in Sabouraud agar with glucose and inhibitors, 8% ovine blood agar and McConkey agar (AMc), and incubated at 37 ºC. Under these conditions, white opaque fungal colonies with light brown pigmentation on the underside developed. Microscopically, the genus Chrysosporium was identified by the observation of septate, straight, irregularly branched hyphae with smooth surface and globose conidia. Histological findings included loss of epithelial continuity with infiltration of granulocytes and the presence of the saprophytic organism. In the dermis, granulomatous lesion with scarce fibroblastic and angioblastic reactions were observed.

Clínica Veterinária, 2020

Eventration is a separation of the musculoaponeurotic layers of the abdominal wall resulting in p... more Eventration is a separation of the musculoaponeurotic layers of the abdominal wall resulting in protrusion of abdominal contents, and characterized by the absence of the hernial sac and intact skin. We describe a case of chronic left sided eventration associated with deformity of the right femoral fossa in a 30-year-old female Argentine land tortoise. The animal was referred for evaluation of a soft and non-painful deformity of the left lumbar fossa and third degree lameness of the left posterior limb. Eventration of one bladder lobe and oviduct loops was diagnosed by ultrasonography. We describe the anesthetic protocol and the surgical technique used in the treatment with approach to the coelomic cavity through the prefemoral fossa, as well as the resolution of the abdominal wall defect.

Reptilia Revista Especializada En Reptiles Anfibios Y Artropodos, 2005

Reptilia Revista Especializada En Reptiles Anfibios Y Artropodos, 2009

Cuadernos De Herpetologia, 1986

Se describen las observaciones hechas en sangre de 30 ejemplares d e tortuga terrestre a r g e n ... more Se describen las observaciones hechas en sangre de 30 ejemplares d e tortuga terrestre a r g e n t i n a (~e o c h e l o n e c h i l e n s i s) , discutiendo l a t é c n i c a adecuada para fa toma de muestras de sangre. Además, se evalfien parénietros de quimica h e r n a t i r~ y se d e t a l l a la morfologfa d e les d i f e r e n t e s series cel u l a r e s , wmparando las datos obtenidos con e s p e c i e s e x d t i c e s afines. WMMARY It is cfescribod #e a b s e r v a t b n s made i n bl*d fmm 3b specimens of a r g e n t i n e tsrrestrieil t u r t l e (&~c h e l o~~ , d i s c u s s i n g t h e a d e w a t e technique f r o m blood sampling. hlloreover it is kvaluated b l w d dtemistry paraf~tslls m d it fs d e t a i led the mrpholagy o? t h e d i f e r e n t s cells series, compared the d a t e s wfth r e l a t e d emctics species. * ~a r d i n ~oo1Ógim de Buenos Aires R e~Ú b l i c a d e la I n d i a 2900, (1425) C a p i t a l Federal Argentina .

Russian Journal of Herpetology, Oct 7, 2011

In order to establish referential hematological parameters, blood samples were taken from 100 hea... more In order to establish referential hematological parameters, blood samples were taken from 100 healthy specimens of argentine tegu lizard ( Tupinambis merinae ) by means of venipuncture of ventral coccigeal vein. The determination of red blood cells, leukocyte and thrombocyte count, hematocrit, hemoglobin concentration, hematometric index and differential leukocyte count were performed and compared with other saurian species. No statistically significant changes were observed as function as sex and age ( p > 0.01). During winter high values of red blood cell counts, hematocrit and hemoglobin were observed ( p < 0.01), while in summer significantly increased leukocyte and thrombocyte counts were observed ( p < 0.01). The differential leukocyte counts were not affected by the studied factors.

Memorias De La Conferencia Interna En Medicina Y Aprovechamiento De Fauna Silvestre Exotica Y No Convencional, Dec 15, 2013

Este documento resume las principales caracteristicas hematologicas de los artiodactilos presente... more Este documento resume las principales caracteristicas hematologicas de los artiodactilos presentes en Suramerica. Destaca aspectos practicos para la colecta de sangre en cervidos y camelidos, asi como anotaciones practicas de las principales diferencias celulares encontradas entre las diferentes especies de mamiferos de este orden taxonomico.

Se tomaron muestras sanguíneas de 150 ejemplares sanos de tortuga terrestre argentina (Chelonoidi... more Se tomaron muestras sanguíneas de 150 ejemplares sanos de tortuga terrestre argentina (Chelonoidis chilensis chilensis) por medio de punción de la vena coccígea superior. Las determinaciones que se realizaron incluyeron recuentos de glóbulos rojos, leucocitos y trombocitos, hematocrito, concentración de hemoglobina, índices hematimétricos y fórmulas leucocitarias relativas, las que se compararon con otras especies de Testudinidae. No se observaron cambios estadísticamente significativos en los parámetros sanguíneos como función de la edad o el sexo (p >0,01). En el invierno se observó un aumento de los valores de glóbulos rojos, hematocrito y hemoglobina (p <0,01), mientras que en le verano aumentaron los glóbulos blancos y trombocitos (p <0,01). La formula leucocitaria no resulto afectada por los factores en estudio.

Toxicon, 2000

Bothrops ammodytoides, the smallest representative of this genus, is found only in Argentina. Ven... more Bothrops ammodytoides, the smallest representative of this genus, is found only in Argentina. Venom was extracted from thirty adult specimens (35±70 cm in length, 90±300 g in weight) captured in the Province of Buenos Aires and kept in captivity. Venom yield was 3±30 mg. SDS-PAGE showed strong bands at 14.0; 23±25; 45; 54 and 63 kDa and weak bands at 17.0; 30.0; 40.0 and 85.0 kDa. Toxic activities were: LD 50 (intravenous, mice) 0.52 0.2 mg/g; minimal procoagulant dose on human plasma (MPD-P) 3522 mg/ l; and minimal de®brinogenating dose (MDD, mice) 6±12 mg. Hemorrhagic and/or necrotic activities appear to play a major role in lethality; minimal hemorrhagic dose (MHD, mice) is 1022 mg/g and minimal necrotizing dose (MND, mice) is 3825 mg. The LD 50 , MPD-P and MND are among the lowest in venoms from Bothrops species found in Argentina. B. ammodytoides venom exhibited high proteolytic and phospholipase A 2 activities. Most of

Journal of Venomous Animals and Toxins including Tropical Diseases, 2006

A comparative study on the sensitivity of erythrocytes from different vertebrate species (avian, ... more A comparative study on the sensitivity of erythrocytes from different vertebrate species (avian, mammalian and reptilian) to the hemolytic action caused by cardiotoxin isolated from Naja naja atra venom was carried out. Cardiotoxin was able to induce direct hemolysis in washed erythrocytes from several animals, except for llama. The EC50 values from hemolysis of the most sensitive (cat) and the most resistant (snake) animal varied approximately tenfold. According to the cell behavior, it was possible to characterize four types of behavior: The first was observed in cat, horse and human cells; the second in rat, rabbit and dog erythrocytes; and the third only in llama erythrocytes, which were resistant to cardiotoxin concentrations up to 300 µg/ml. Finally, avian and reptilian erythrocytes were more resistant to cardiotoxin III-induced hemolysis than those of the mammalian species.

Comparative Clinical Pathology, 2010

... Red blood cells, total leukocyte count and thrombocyte count were performed in a 1:2,000 dilu... more ... Red blood cells, total leukocyte count and thrombocyte count were performed in a 1:2,000 dilution of blood in a solution as described by Natt and Herrick (1952), which allows erythrocytes, leukocytes and thrombocytes to be counted in the same standard ... 2005; Carvalho et al. ...

Comparative Clinical Pathology, 2008

... Red blood cells, total leukocyte and thrombocyte counts were performed in a 1:200 dilution of... more ... Red blood cells, total leukocyte and thrombocyte counts were performed in a 1:200 dilution of blood in a solution as described by Natt and Herrick (1952 ... The type of blood cells found in this lizard species agree with information reported in the same species by Carvalho et al. ...

Rev. Salud Anim. Vol, 2004

Introducción Los dos grandes registros multicéntricos de cirugía cardíaca realizados en la Argent... more Introducción Los dos grandes registros multicéntricos de cirugía cardíaca realizados en la Argentina, CONAREC y ESMUCICA, datan de más de 10 años. Considerando los avances médicos y quirúrgicos de la última década, surgió la necesidad de realizar un nuevo registro nacional, prospectivo y multicéntrico para conocer las características, la evolución, las complicaciones y los predictores de mortalidad de los pacientes sometidos a cirugía cardíaca. Objetivos Conocer el perfil epidemiológico, la modalidad quirúrgica y la evolución posoperatoria de los pacientes sometidos a cirugía cardíaca en la Argentina. Material y métodos Registro prospectivo, consecutivo y multicéntrico realizado en 49 centros cardioquirúrgicos de la República Argentina por residentes de cardiología. Se analizaron las características y la evolución de 2.553 pacientes sometidos a cirugía cardíaca entre septiembre de 2007 y octubre de 2008: 1.465 (57,4%) a cirugía coronaria, 359 (14,1%) a reemplazo valvular aórtico, 169 (6,6%) a cirugía valvular mitral, 312 (12,2%) a cirugía combinada coronariovalvular y 248 (9,7%) a otros procedimientos. Resultados Hubo predominio de hombres (74,9%); la edad promedio fue de 63 ± 11 años. La prevalencia de diabetes fue del 24,9%, la de hipertensión del 76,3% y la de insuficiencia cardíaca del 17%. La disfunción ventricular moderada a grave prequirúrgica fue del 23,8% y el 19,8% de las cirugías fueron no programadas. En las cirugías coronarias, el 41,9% de ellas se realizaron sin circulación extracorpórea y se empleó puente mamario en el 89%. El 81,7% de las cirugías mitrales se indicaron por insuficiencia y el 62,6% de las aórticas, por estenosis. En estas cirugías se emplearon válvulas mecánicas en el 58% de los casos. La mediana de internación fue de 6 días. Se presentaron complicaciones mayores en el 31,7% (del 25% en coronarios al 49,36% en combinados) y la mortalidad global fue del 7,7% (del 4,3% en coronarios al 13,4% en combinados). Conclusiones Este registro muestra la realidad de la cirugía cardíaca en centros con residencia o concurrencia de cardiología. Comparadas con cifras de registros nacionales previos, la mortalidad y las complicaciones mayores han disminuido, pero continúan siendo elevadas.