Desy Wulandari | University of Brawijaya (original) (raw)

Papers by Desy Wulandari

Pediatrics Sciences Journal, 2022

Introduction: House dust mite (HDM) allergy has been reported as an actual cause of asthma in chi... more Introduction: House dust mite (HDM) allergy has been reported as an actual cause of asthma in children. Subcutaneous immunotherapy (SCIT) is a recommended treatment for HDM allergy patients. However, there were limited data about the characteristic of pediatric patients with HDM-SCIT, particularly in Indonesia. This study was aimed to evaluate the characteristic of pediatric patients with allergic asthma. Methods: Study participants were pediatric patients confirmed with HDM allergy from Skin Prick Test (SPT) in the pediatric allergy-immunology outpatient clinic in Saiful Anwar Hospital Malang, Indonesia. Patients who were treated with HDM-SCIT in the early build-up phase of treatment were included in this study. Demographic and clinical characteristics of the patients were recorded. Peripheral blood samples were drawn to evaluate the total eosinophil count (TEC), total basophil count (TBC), neutrophil-lymphocyte ratio (NLR), specific IgE (sIgE) and total IgE (t-IgE) level. Clinical...

Pediatrics Sciences Journal, 2022

Introduction: The prospective studies have estimated that the DRESS syndrome incidence rate is ab... more Introduction: The prospective studies have estimated that the DRESS syndrome incidence rate is about 1 per 1000 to 10,000 with the mortality rate is to be around 10% to 20%. Case: We have been reported a case of DRESS in an eleven-year-old boy who came to the emergency room with the chief complaint of maculopapular rash in the body that had been happened for three days. The rash was first appeared in the arms area and thus spread to the trunk, abdomen, and lower extremities. The erythema was well-demarcated, purplish in color. There was a history of fever a week before admission which reached 40 0 Celcius. There are multiple histories of drug administration, either orally or intravenously to this patient, and made the diagnosis of drug reaction is highly possible. The management of these patients consists of suspending suspected drugs and implementing supportive measures. Conclusion: Regarding pharmacological treatment, the use of systemic corticosteroids for a prolonged period with...

Atopic Dermatitis: Improving Early Detection and Prevention Measures in the Community Setting

Caring Jurnal Pengabdian Masyarakat, 2021

Atopic dermatitis is a chronic inflammation of the skin characterized by disruption of the skin b... more Atopic dermatitis is a chronic inflammation of the skin characterized by disruption of the skin barrier and aberrations in the immune response. In general, atopic dermatitis can be affected by various complex interactions including genetics, diet, and stress. The lack of public attention to atopic dermatitis which often affects children is one of the factors for the increasing prevalence of atopic dermatitis. The purpose of this community service is to help the community understand the importance of early detection and allergy prevention efforts. Community service is carried out by holding online seminars that can be followed by the general public as well as direct consultations and discussions with pediatricians for allergy patients at the Department of Pediatrics at Dr. RSUD Dr. Saiful Anwar Malang. The total Immunoglobulin E level of the respondents was measured using the enzyme-linked immunosorbent assay method. The severity of the respondents was determined by calculating the S...

Improvement of Th17/Treg balance and Asthma Control Test score by Nigella sativa supplementation in asthmatic children: a new approach to managing asthma

Turkish Journal of Immunology, 2018

Introduction: Th17 and Treg cells have important roles in the development and progression of the ... more Introduction: Th17 and Treg cells have important roles in the development and progression of the allergic diseases, such as asthma. Several studies reported that Nigella sativa could improve chronic airway inflammation, and inhibit airway remodeling by regulating the immune system. To examine the effect of Nigella sativa supplementation on Th17/ Treg cells, and asthma control test score in asthmatic children. Materials and Methods: Twenty-eight children with asthma meeting the inclusion criteria in Saiful Anwar Hospital, Indonesia, participated in this study. All subjects took the standard treatment based on asthma guidelines. Nigella sativa oil (NSO) is given as additional treatment at the dose of 15–30 mg/kg/day for eight weeks in a randomized, singleblind-controlled trial. Th17 and Treg in peripheral blood mononuclear cells were analyzed by flow cytometry tool. Improvement of asthma control was accessed by ACT (Asthma Control Test) Score. Results: There was a significant decrease of Th17, and an increase of Treg percentages in NSO treatment group. Th17/ Treg ratio was lower in NSO group compared to the standard treatment group (1.06±0.33 vs. 2.30±1.08, p=0.001). The ACT score improvement was significantly higher in NSO compared to that in the standard treatment group (3.71±1.634 vs. 1.86±0.864, p=0.033). However, there was no significant correlation between Th17/Treg ratio and ACT score in preand post-treatment (p=0.552, p=0.344 respectively). Conclusion: NSO supplementation improves Th17/Treg balance and clinical symptoms in asthmatic children.

Paediatrica Indonesiana, 2018

Background Asthma is a chronic inflammatory disease of the airways characterized by involvement o... more Background Asthma is a chronic inflammatory disease of the airways characterized by involvement of a variety of inflammatory cells. Asthma is associated with imbalances between Th1/Th2 cells and their characteristic cytokine profiles. Nigella sativa is a plant that possesses immunomodulatory and anti-inflammatory properties.Objective To investigate the potential anti-asthmatic effect of Nigella sativa oil on Th1/Th2 cells, IFN-ɣ/IL-4 cytokines, and improvement of asthma control.Methods Children aged 6-15 years with asthma in Dr. Saiful Anwar Hospital, Malang, were enrolled in this study. All patients were treated based on standard treatment guidelines for asthma. Nigella sativa oil (NSO) was given per oral as supplementary treatment at a dose of 15-30 mg/kg/day for 8 weeks, in a randomized, single-blind, controlled trial. Peripheral Th1 and Th2 cells were counted by flow cytometry and IFN-ɣ and IL-4 cytokines were measured by ELISA. Improvement of asthma control was assessed by the ...

The Indonesian Biomedical Journal, 2020

BACKGROUND: Vitamin D plays essential role in the regulation of inflammation, such as in pathogen... more BACKGROUND: Vitamin D plays essential role in the regulation of inflammation, such as in pathogenesis of Juvenile Idiopathic Arthritis (JIA). Vitamin D deficiency has been reported among JIA patients, but there were conflicting results regarding the correlation with disease activity. This study aimed to assess vitamin D serum level and its correlation with C-Reactive Protein (CRP) and disease activity in JIA patients.METHODS: Children who were diagnosed with JIA according to International League of Associations for Rheumatology (ILAR) criterias were enrolled as JIA group subjects, while age and sex-matched healthy children were enrolled as the control group subjects. Vitamin D and CRP serum level were measured. Disease activity of JIA patients was calculated by Juvenile Arthritis Disease ActivityScore-27 (JADAS-27).RESULTS: Vitamin D serum level was lower in the JIA group compared to the healthy control group (p=0.000). Among 26 JIA patients, 61.5% were deficient, 30.8% were insuffi...

Low Levels of Vitamin D correlate with Hemoglobin A1c and Interleukin-10 Levels in Pediatric Type 1 Diabetes Mellitus Patients

Journal of Tropical Life Science, Dec 24, 2014

Primary immunodeficiency disorder (PID) refers to a heterogeneous group of over 130 disorders tha... more Primary immunodeficiency disorder (PID) refers to a heterogeneous group of over 130 disorders that result from defects in immune system development and/or function. PIDs are broadly classified as disorders of adaptive immunity (i.e., T-cell, B-cell or combined immunodeficiencies) or of innate immunity (e.g., phagocyte and complement disorders). Although the clinical manifestations of PIDs are highly variable, most disorders involve at least an increased susceptibility to infection. Early diagnosis and treatment are imperative for preventing significant disease-associated morbidity and, therefore, consultation with a clinical immunologist is essential. PIDs should be suspected in patients with: recurrent sinus or ear infections or pneumonias within a 1 year period; failure to thrive; poor response to prolonged use of antibiotics; persistent thrush or skin abscesses; or a family history of PID. Patients with multiple autoimmune diseases should also be evaluated. Diagnostic testing often involves lymphocyte proliferation assays, flow cytometry, measurement of serum immunoglobulin (Ig) levels, assessment of serum specific antibody titers in response to vaccine antigens, neutrophil function assays, stimulation assays for cytokine responses, and complement studies. The treatment of PIDs is complex and generally requires both supportive and definitive strategies. Ig replacement therapy is the mainstay of therapy for B-cell disorders, and is also an important supportive treatment for many patients with combined immunodeficiency disorders. The heterogeneous group of disorders involving the T-cell arm of the adaptive system, such as severe combined immunodeficiency (SCID), require immune reconstitution as soon as possible. The treatment of innate immunodeficiency disorders varies depending on the type of defect, but may involve antifungal and antibiotic prophylaxis, cytokine replacement, vaccinations and bone marrow transplantation. This article provides a detailed overview of the major categories of PIDs and strategies for the appropriate diagnosis and management of these rare disorders.

Pediatrics Sciences Journal, 2022

Introduction: House dust mite (HDM) allergy has been reported as an actual cause of asthma in chi... more Introduction: House dust mite (HDM) allergy has been reported as an actual cause of asthma in children. Subcutaneous immunotherapy (SCIT) is a recommended treatment for HDM allergy patients. However, there were limited data about the characteristic of pediatric patients with HDM-SCIT, particularly in Indonesia. This study was aimed to evaluate the characteristic of pediatric patients with allergic asthma. Methods: Study participants were pediatric patients confirmed with HDM allergy from Skin Prick Test (SPT) in the pediatric allergy-immunology outpatient clinic in Saiful Anwar Hospital Malang, Indonesia. Patients who were treated with HDM-SCIT in the early build-up phase of treatment were included in this study. Demographic and clinical characteristics of the patients were recorded. Peripheral blood samples were drawn to evaluate the total eosinophil count (TEC), total basophil count (TBC), neutrophil-lymphocyte ratio (NLR), specific IgE (sIgE) and total IgE (t-IgE) level. Clinical...

Pediatrics Sciences Journal, 2022

Introduction: The prospective studies have estimated that the DRESS syndrome incidence rate is ab... more Introduction: The prospective studies have estimated that the DRESS syndrome incidence rate is about 1 per 1000 to 10,000 with the mortality rate is to be around 10% to 20%. Case: We have been reported a case of DRESS in an eleven-year-old boy who came to the emergency room with the chief complaint of maculopapular rash in the body that had been happened for three days. The rash was first appeared in the arms area and thus spread to the trunk, abdomen, and lower extremities. The erythema was well-demarcated, purplish in color. There was a history of fever a week before admission which reached 40 0 Celcius. There are multiple histories of drug administration, either orally or intravenously to this patient, and made the diagnosis of drug reaction is highly possible. The management of these patients consists of suspending suspected drugs and implementing supportive measures. Conclusion: Regarding pharmacological treatment, the use of systemic corticosteroids for a prolonged period with...

Atopic Dermatitis: Improving Early Detection and Prevention Measures in the Community Setting

Caring Jurnal Pengabdian Masyarakat, 2021

Atopic dermatitis is a chronic inflammation of the skin characterized by disruption of the skin b... more Atopic dermatitis is a chronic inflammation of the skin characterized by disruption of the skin barrier and aberrations in the immune response. In general, atopic dermatitis can be affected by various complex interactions including genetics, diet, and stress. The lack of public attention to atopic dermatitis which often affects children is one of the factors for the increasing prevalence of atopic dermatitis. The purpose of this community service is to help the community understand the importance of early detection and allergy prevention efforts. Community service is carried out by holding online seminars that can be followed by the general public as well as direct consultations and discussions with pediatricians for allergy patients at the Department of Pediatrics at Dr. RSUD Dr. Saiful Anwar Malang. The total Immunoglobulin E level of the respondents was measured using the enzyme-linked immunosorbent assay method. The severity of the respondents was determined by calculating the S...

Improvement of Th17/Treg balance and Asthma Control Test score by Nigella sativa supplementation in asthmatic children: a new approach to managing asthma

Turkish Journal of Immunology, 2018

Introduction: Th17 and Treg cells have important roles in the development and progression of the ... more Introduction: Th17 and Treg cells have important roles in the development and progression of the allergic diseases, such as asthma. Several studies reported that Nigella sativa could improve chronic airway inflammation, and inhibit airway remodeling by regulating the immune system. To examine the effect of Nigella sativa supplementation on Th17/ Treg cells, and asthma control test score in asthmatic children. Materials and Methods: Twenty-eight children with asthma meeting the inclusion criteria in Saiful Anwar Hospital, Indonesia, participated in this study. All subjects took the standard treatment based on asthma guidelines. Nigella sativa oil (NSO) is given as additional treatment at the dose of 15–30 mg/kg/day for eight weeks in a randomized, singleblind-controlled trial. Th17 and Treg in peripheral blood mononuclear cells were analyzed by flow cytometry tool. Improvement of asthma control was accessed by ACT (Asthma Control Test) Score. Results: There was a significant decrease of Th17, and an increase of Treg percentages in NSO treatment group. Th17/ Treg ratio was lower in NSO group compared to the standard treatment group (1.06±0.33 vs. 2.30±1.08, p=0.001). The ACT score improvement was significantly higher in NSO compared to that in the standard treatment group (3.71±1.634 vs. 1.86±0.864, p=0.033). However, there was no significant correlation between Th17/Treg ratio and ACT score in preand post-treatment (p=0.552, p=0.344 respectively). Conclusion: NSO supplementation improves Th17/Treg balance and clinical symptoms in asthmatic children.

Paediatrica Indonesiana, 2018

Background Asthma is a chronic inflammatory disease of the airways characterized by involvement o... more Background Asthma is a chronic inflammatory disease of the airways characterized by involvement of a variety of inflammatory cells. Asthma is associated with imbalances between Th1/Th2 cells and their characteristic cytokine profiles. Nigella sativa is a plant that possesses immunomodulatory and anti-inflammatory properties.Objective To investigate the potential anti-asthmatic effect of Nigella sativa oil on Th1/Th2 cells, IFN-ɣ/IL-4 cytokines, and improvement of asthma control.Methods Children aged 6-15 years with asthma in Dr. Saiful Anwar Hospital, Malang, were enrolled in this study. All patients were treated based on standard treatment guidelines for asthma. Nigella sativa oil (NSO) was given per oral as supplementary treatment at a dose of 15-30 mg/kg/day for 8 weeks, in a randomized, single-blind, controlled trial. Peripheral Th1 and Th2 cells were counted by flow cytometry and IFN-ɣ and IL-4 cytokines were measured by ELISA. Improvement of asthma control was assessed by the ...

The Indonesian Biomedical Journal, 2020

BACKGROUND: Vitamin D plays essential role in the regulation of inflammation, such as in pathogen... more BACKGROUND: Vitamin D plays essential role in the regulation of inflammation, such as in pathogenesis of Juvenile Idiopathic Arthritis (JIA). Vitamin D deficiency has been reported among JIA patients, but there were conflicting results regarding the correlation with disease activity. This study aimed to assess vitamin D serum level and its correlation with C-Reactive Protein (CRP) and disease activity in JIA patients.METHODS: Children who were diagnosed with JIA according to International League of Associations for Rheumatology (ILAR) criterias were enrolled as JIA group subjects, while age and sex-matched healthy children were enrolled as the control group subjects. Vitamin D and CRP serum level were measured. Disease activity of JIA patients was calculated by Juvenile Arthritis Disease ActivityScore-27 (JADAS-27).RESULTS: Vitamin D serum level was lower in the JIA group compared to the healthy control group (p=0.000). Among 26 JIA patients, 61.5% were deficient, 30.8% were insuffi...

Low Levels of Vitamin D correlate with Hemoglobin A1c and Interleukin-10 Levels in Pediatric Type 1 Diabetes Mellitus Patients

Journal of Tropical Life Science, Dec 24, 2014

Primary immunodeficiency disorder (PID) refers to a heterogeneous group of over 130 disorders tha... more Primary immunodeficiency disorder (PID) refers to a heterogeneous group of over 130 disorders that result from defects in immune system development and/or function. PIDs are broadly classified as disorders of adaptive immunity (i.e., T-cell, B-cell or combined immunodeficiencies) or of innate immunity (e.g., phagocyte and complement disorders). Although the clinical manifestations of PIDs are highly variable, most disorders involve at least an increased susceptibility to infection. Early diagnosis and treatment are imperative for preventing significant disease-associated morbidity and, therefore, consultation with a clinical immunologist is essential. PIDs should be suspected in patients with: recurrent sinus or ear infections or pneumonias within a 1 year period; failure to thrive; poor response to prolonged use of antibiotics; persistent thrush or skin abscesses; or a family history of PID. Patients with multiple autoimmune diseases should also be evaluated. Diagnostic testing often involves lymphocyte proliferation assays, flow cytometry, measurement of serum immunoglobulin (Ig) levels, assessment of serum specific antibody titers in response to vaccine antigens, neutrophil function assays, stimulation assays for cytokine responses, and complement studies. The treatment of PIDs is complex and generally requires both supportive and definitive strategies. Ig replacement therapy is the mainstay of therapy for B-cell disorders, and is also an important supportive treatment for many patients with combined immunodeficiency disorders. The heterogeneous group of disorders involving the T-cell arm of the adaptive system, such as severe combined immunodeficiency (SCID), require immune reconstitution as soon as possible. The treatment of innate immunodeficiency disorders varies depending on the type of defect, but may involve antifungal and antibiotic prophylaxis, cytokine replacement, vaccinations and bone marrow transplantation. This article provides a detailed overview of the major categories of PIDs and strategies for the appropriate diagnosis and management of these rare disorders.