Carmen Campino | Pontificia Universidad Catolica de Chile (original) (raw)
Papers by Carmen Campino
Journal of Hypertension, 2016
s e329 Tualang Honey (TH) contains high level of antioxidant. However, its effects on hypertensio... more s e329 Tualang Honey (TH) contains high level of antioxidant. However, its effects on hypertension is unclear. Thus, the aim of this study was to evaluate the protective effects of TH on endothelial cell barrier in response to oxidative stress in vitro. Design and method: To measure endothelial function in vitro, vascular permeability assay was performed using human umbilical vein endothelial cells (HUVECs) on transwell inserts. HUVECs were pre-treated with TH (concentration ranging from 0.01% to 1%) for 4 hours, then followed by oxidative stress with hydrogen peroxide (H 2 O 2) for 60 mins. The level of damage of the endothelial cell junction by H 2 O 2 was measured based on the amount of FITC-dextran that passed through the HUVEC layer from the transwell insert to the bottom well. Changes in HUVEC phenotypes were confi rmed by immunofl uorescence staining for actin cytoskeleton, beta-catenin and caveolin-1. Trolox was used as a reference antioxidant compound in this experiment. Results: Our results showed that H 2 O 2 increased HUVEC permeability but the effect was signifi cantly suppressed by TH, which coupled with signifi cant attenuation of actin fi lament remodeling in HUVECs. In addition, TH was also found to salvage HUVEC adherence junction (beta-catenin) and signifi cantly down-regulated its caveolin-1 production in response to H 2 O 2. The highest inhibition was observed in groups treated with 0.1% TH and the level was comparable to that of Trolox-treated. Conclusions: Our data shows that TH could inhibit H 2 O 2-induced endothelial permeability by suppressing the actin remodeling, caveolin-1 production and attenuating disruption of endothelial adherence junctions. This suggests that TH possesses therapeutic potential for treating hypertension-associated endothelial dysfunction related diseases. Therefore, further studies to investigate its therapeutic effects and mechanism of action in vivo are warranted.
PubMed, Jun 1, 1999
Background: Thyroglobulin measurement is useful for the follow up of patients subjected to total ... more Background: Thyroglobulin measurement is useful for the follow up of patients subjected to total thyroidectomy for differentiated thyroid carcinoma. Thyroglobulin autoantibodies may interfere with its determination. Aim: To measure thyroglobulin autoantibodies and their interference with thyroglobulin determination. Material and methods: The presence of thyroglobulin autoantibodies was investigated in 801 serum samples sent to the laboratory for measurement of thyroglobulin levels. A serum was considered positive for these autoantibodies when radioactivity corresponding to 125I-thyroglobulin bound to thyroglobulin autoantibodies, precipitated with human gamma globulin, exceeded in 1.4 times that of a negative sera pool. In positive sera, thyroglobulin autoantibody concentration was measured and its interference with thyroglobulin radioimmunoassay was assessed through a recuperation test using exogenous thyroglobulin. Results: Thyroglobulin autoantibodies were detected in 149 sera (18.6%). Of these, 65 had a recuperation that fluctuated between 1 and 80%. Thyroglobulin autoantibody concentration was negatively correlated with recuperation percentages (r = -0.64; p < 0.001) but not with thyroglobulin concentrations (r = 0.08). Thyroglobulin was higher in positive sera with a recuperation over 80% than in sera with a recuperation of less than 80% (12.7 +/- 1.7 and 5.9 +/- 0.6 ng/ml, respectively; p < 0.001). Conclusions: Thyroglobulin autoantibodies interfere with thyroglobulin measurement by radioimmunoassay, sequestering variable amounts of thyroglobulin. The presence of these autoantibodies must be investigated prior to thyroglobulin determination.
The Journal of Clinical Endocrinology and Metabolism, Sep 14, 2018
Context: Classical apparent mineralocorticoid excess (AME) is a rare recessive disorder, caused b... more Context: Classical apparent mineralocorticoid excess (AME) is a rare recessive disorder, caused by severe 11b-hydroxysteroid dehydrogenase type 2 enzyme (11b-HSD2) deficiency. AME manifests as low-renin pediatric hypertension, hypokalemia and high cortisol/cortisone (F/E) ratio. Objective: To evaluate nonclassic AME (NC-AME) due to partial 11b-HSD2 insufficiency and its association with hypertension, mineralocorticoid receptor (MR) activation, and inflammatory parameters.
59th ESPE Annual Meeting (ESPE 2021 Online), 2021
58th Annual ESPE Meeting (ESPE 2019), 2019
Higher dehydroepiandrosterone levels in prepubertal children born very preterm and adequate for g... more Higher dehydroepiandrosterone levels in prepubertal children born very preterm and adequate for gestational age Verónica Mericq1, Alejandro Martinez-Aguayo2, German Iñiguez1, Helena Poggi2, Ivonne D'Apremont2,3, Rosario Moore2, Mónica Arancibia2,4, Hernán Garcia2, Soledad Peredo2, Claudia Trincado2, Sofía Sifaqui2, José Tomas Ossa2, Carlos Fardella5, Cristian Carvajal5, Carmen Campino5, Rene Baudrand5, Sandra Solari6, Fidel Allende6. 1Institute of Maternal and Child Research, University of Chile, Santiago. 2Division of Pediatrics, Pontificia Universidad Católica de Chile. 3Complejo Asistencial Hospital Dr. Sotero del Rio, Santiago de Chile, Chile. 4Servicio de Pediatría, Hospital Higueras, Talcahuano, Chile. 5Endocrinology Department, Pontificia Universidad Catolica de Chile. 6Department of Clinical Laboratories, Pontificia Universidad Catolica de Chile, Santiago, Chile
Neuro endocrinology letters, 2018
BACKGROUND Cortisol dysregulation has a potential role in depression. AIM AND METHODS We evaluate... more BACKGROUND Cortisol dysregulation has a potential role in depression. AIM AND METHODS We evaluated depressive symptoms using the Hamilton Rating Scale for Depression in 48 primary care subjects without history of previous or current depression and its association with cortisol dysregulation (morning plasma cortisol, 24-hour urinary free cortisol and cortisol metabolites). Presence of metabolic syndrome and inflammatory parameters were also assessed. RESULTS Hamilton Rating Scale for Depression correlated significantly with morning cortisol, but not with urinary free cortisol or metabolites. A significant increase in morning cortisol by Hamilton groups (asymptomatic ≤8; mild to moderate: 9-18; moderate to severe: ≥19) was observed even when adjusted by age/gender. We observed no association of depressive symptoms with metabolic or inflammatory parameters. CONCLUSION Depressive symptoms in primary care subjects not consulting for their mood are associated with higher morning plasma co...
Insulin resistance parameters in children who were born very preterm and adequate for gestational... more Insulin resistance parameters in children who were born very preterm and adequate for gestational age Hernán Garcia1, Helena Poggi1, Ivonne D'Apremont1,2, Rosario Moore1, Mónica Arancibia1,2, Soledad Peredo1, Claudia Trincado1, Sofía Sifaqui1, José Tomas Ossa1, Carlos Fardella4, Cristian Carvajal4, Carmen Campino4, Rene Baudrand4, Sandra Solari5, Fidel Allende5,Alejandro Martinez-Aguayo1. 1Division of Pediatrics, Pontificia Universidad Católica de Chile. 2Complejo Asistencial Hospital Dr. Sotero del Rio, Santiago de Chile, Chile. 3Servicio de Pediatría, Hospital Higueras, Talcahuano, Chile. 4Endocrinology Department, Pontificia Universidad Catolica de Chile. 5Department of Clinical Laboratories, Pontificia Universidad Catolica de Chile, Santiago, Chile
About 15% of the essential hypertensive patients would have a low activity of the 11βHSD2 enzyme,... more About 15% of the essential hypertensive patients would have a low activity of the 11βHSD2 enzyme, which inactivates cortisol (F) to cortisone (E). Gene expression can be negatively regulated by miRNA. Urinary exosomes and their specific content (miRNA/proteins) represent a valuable tool as a biomarker for the diagnosis and prognosis of the disease. Aim: To evaluate the expression of miRNA specific for 11βHSD2 in samples of urinary exosomes and to determine its association with biochemical variables associated with mineralocorticoid metabolism. Subjects and Methods: Cross-sectional study in subjects between 10-60 years. They were classified into subjects with high F/E (> p75) and low cortisone (< p25) and control subjects. The urinary exosomes were isolated with the Invitrogen kit. Bioinformatic analysis was performed with MirWalk to identify specific miRNAs of HSD11B2. The expression of miRNA was evaluated by qRTPCR. The comparisons were made with the Mann-Whitney test. Result...
Journal of Hypertension, 2019
Objectives: To identify associations among the plasma renin concentration (PRC), plasma aldostero... more Objectives: To identify associations among the plasma renin concentration (PRC), plasma aldosterone and urinary sodium (Na)/potassium (K) ratio, and to integrate these variables into a nomogram with the aim of estimating the expected versus observed aldosterone concentration. Methods: We studied 40 healthy normotensive children (5–8 years old, 57.5% girls) who were born at term and were adequate for their gestational age. Following overnight fasting, the PRC and plasma aldosterone in blood samples were measured, and the Na/K ratio was calculated from a simultaneously obtained urinary spot sample. A mathematical function was defined with these three variables, and a nomogram was built that would return the expected aldosterone concentration from the obtained plasma renin and urinary Na/K ratio values. Results: The PRC (B = 5.9, P < 0.001) and urinary Na/K ratio (B = −98.1, P = 0.003) were significant independent predictors of plasma aldosterone. The correlation between the observ...
American Journal of Hypertension, 2019
BACKGROUND Aldosterone has been linked with obesity, metabolic syndrome (MetS), pro-inflammatory,... more BACKGROUND Aldosterone has been linked with obesity, metabolic syndrome (MetS), pro-inflammatory, and prothrombotic states; however, most studies relate these indicators with primary aldosteronism (PA), excluding non-PA patients. OBJECTIVE To determine whether aldosterone, renin, or the plasma aldosterone/renin ratio (ARR) are associated with metabolic disorders and inflammatory/vascular biomarkers in a non-PA population. METHODS We studied 275 patients including adolescents and adults of both genders and measured plasma and urinary aldosterone and determined the plasma renin activity. In all subjects, the presence of MetS was determined according to Adult Treatment Panel III. Renal, vascular, inflammatory, and mineralocorticoid activity biomarkers were evaluated. RESULTS The ARR correlated with the number of variables of MetS (r = 0.191, P = 0.002), body mass index (BMI; r = 0.136, P = 0.026), systolic blood pressure (r = 0.183, P = 0.002), diastolic blood pressure (r = 0.1917, P =...
The Endocrine Society's 92nd Annual Meeting, June 19–22, 2010 - San Diego, 2010
Journal of Hypertension, 2016
s e329 Tualang Honey (TH) contains high level of antioxidant. However, its effects on hypertensio... more s e329 Tualang Honey (TH) contains high level of antioxidant. However, its effects on hypertension is unclear. Thus, the aim of this study was to evaluate the protective effects of TH on endothelial cell barrier in response to oxidative stress in vitro. Design and method: To measure endothelial function in vitro, vascular permeability assay was performed using human umbilical vein endothelial cells (HUVECs) on transwell inserts. HUVECs were pre-treated with TH (concentration ranging from 0.01% to 1%) for 4 hours, then followed by oxidative stress with hydrogen peroxide (H 2 O 2) for 60 mins. The level of damage of the endothelial cell junction by H 2 O 2 was measured based on the amount of FITC-dextran that passed through the HUVEC layer from the transwell insert to the bottom well. Changes in HUVEC phenotypes were confi rmed by immunofl uorescence staining for actin cytoskeleton, beta-catenin and caveolin-1. Trolox was used as a reference antioxidant compound in this experiment. Results: Our results showed that H 2 O 2 increased HUVEC permeability but the effect was signifi cantly suppressed by TH, which coupled with signifi cant attenuation of actin fi lament remodeling in HUVECs. In addition, TH was also found to salvage HUVEC adherence junction (beta-catenin) and signifi cantly down-regulated its caveolin-1 production in response to H 2 O 2. The highest inhibition was observed in groups treated with 0.1% TH and the level was comparable to that of Trolox-treated. Conclusions: Our data shows that TH could inhibit H 2 O 2-induced endothelial permeability by suppressing the actin remodeling, caveolin-1 production and attenuating disruption of endothelial adherence junctions. This suggests that TH possesses therapeutic potential for treating hypertension-associated endothelial dysfunction related diseases. Therefore, further studies to investigate its therapeutic effects and mechanism of action in vivo are warranted.
PubMed, Jun 1, 1999
Background: Thyroglobulin measurement is useful for the follow up of patients subjected to total ... more Background: Thyroglobulin measurement is useful for the follow up of patients subjected to total thyroidectomy for differentiated thyroid carcinoma. Thyroglobulin autoantibodies may interfere with its determination. Aim: To measure thyroglobulin autoantibodies and their interference with thyroglobulin determination. Material and methods: The presence of thyroglobulin autoantibodies was investigated in 801 serum samples sent to the laboratory for measurement of thyroglobulin levels. A serum was considered positive for these autoantibodies when radioactivity corresponding to 125I-thyroglobulin bound to thyroglobulin autoantibodies, precipitated with human gamma globulin, exceeded in 1.4 times that of a negative sera pool. In positive sera, thyroglobulin autoantibody concentration was measured and its interference with thyroglobulin radioimmunoassay was assessed through a recuperation test using exogenous thyroglobulin. Results: Thyroglobulin autoantibodies were detected in 149 sera (18.6%). Of these, 65 had a recuperation that fluctuated between 1 and 80%. Thyroglobulin autoantibody concentration was negatively correlated with recuperation percentages (r = -0.64; p < 0.001) but not with thyroglobulin concentrations (r = 0.08). Thyroglobulin was higher in positive sera with a recuperation over 80% than in sera with a recuperation of less than 80% (12.7 +/- 1.7 and 5.9 +/- 0.6 ng/ml, respectively; p < 0.001). Conclusions: Thyroglobulin autoantibodies interfere with thyroglobulin measurement by radioimmunoassay, sequestering variable amounts of thyroglobulin. The presence of these autoantibodies must be investigated prior to thyroglobulin determination.
The Journal of Clinical Endocrinology and Metabolism, Sep 14, 2018
Context: Classical apparent mineralocorticoid excess (AME) is a rare recessive disorder, caused b... more Context: Classical apparent mineralocorticoid excess (AME) is a rare recessive disorder, caused by severe 11b-hydroxysteroid dehydrogenase type 2 enzyme (11b-HSD2) deficiency. AME manifests as low-renin pediatric hypertension, hypokalemia and high cortisol/cortisone (F/E) ratio. Objective: To evaluate nonclassic AME (NC-AME) due to partial 11b-HSD2 insufficiency and its association with hypertension, mineralocorticoid receptor (MR) activation, and inflammatory parameters.
59th ESPE Annual Meeting (ESPE 2021 Online), 2021
58th Annual ESPE Meeting (ESPE 2019), 2019
Higher dehydroepiandrosterone levels in prepubertal children born very preterm and adequate for g... more Higher dehydroepiandrosterone levels in prepubertal children born very preterm and adequate for gestational age Verónica Mericq1, Alejandro Martinez-Aguayo2, German Iñiguez1, Helena Poggi2, Ivonne D'Apremont2,3, Rosario Moore2, Mónica Arancibia2,4, Hernán Garcia2, Soledad Peredo2, Claudia Trincado2, Sofía Sifaqui2, José Tomas Ossa2, Carlos Fardella5, Cristian Carvajal5, Carmen Campino5, Rene Baudrand5, Sandra Solari6, Fidel Allende6. 1Institute of Maternal and Child Research, University of Chile, Santiago. 2Division of Pediatrics, Pontificia Universidad Católica de Chile. 3Complejo Asistencial Hospital Dr. Sotero del Rio, Santiago de Chile, Chile. 4Servicio de Pediatría, Hospital Higueras, Talcahuano, Chile. 5Endocrinology Department, Pontificia Universidad Catolica de Chile. 6Department of Clinical Laboratories, Pontificia Universidad Catolica de Chile, Santiago, Chile
Neuro endocrinology letters, 2018
BACKGROUND Cortisol dysregulation has a potential role in depression. AIM AND METHODS We evaluate... more BACKGROUND Cortisol dysregulation has a potential role in depression. AIM AND METHODS We evaluated depressive symptoms using the Hamilton Rating Scale for Depression in 48 primary care subjects without history of previous or current depression and its association with cortisol dysregulation (morning plasma cortisol, 24-hour urinary free cortisol and cortisol metabolites). Presence of metabolic syndrome and inflammatory parameters were also assessed. RESULTS Hamilton Rating Scale for Depression correlated significantly with morning cortisol, but not with urinary free cortisol or metabolites. A significant increase in morning cortisol by Hamilton groups (asymptomatic ≤8; mild to moderate: 9-18; moderate to severe: ≥19) was observed even when adjusted by age/gender. We observed no association of depressive symptoms with metabolic or inflammatory parameters. CONCLUSION Depressive symptoms in primary care subjects not consulting for their mood are associated with higher morning plasma co...
Insulin resistance parameters in children who were born very preterm and adequate for gestational... more Insulin resistance parameters in children who were born very preterm and adequate for gestational age Hernán Garcia1, Helena Poggi1, Ivonne D'Apremont1,2, Rosario Moore1, Mónica Arancibia1,2, Soledad Peredo1, Claudia Trincado1, Sofía Sifaqui1, José Tomas Ossa1, Carlos Fardella4, Cristian Carvajal4, Carmen Campino4, Rene Baudrand4, Sandra Solari5, Fidel Allende5,Alejandro Martinez-Aguayo1. 1Division of Pediatrics, Pontificia Universidad Católica de Chile. 2Complejo Asistencial Hospital Dr. Sotero del Rio, Santiago de Chile, Chile. 3Servicio de Pediatría, Hospital Higueras, Talcahuano, Chile. 4Endocrinology Department, Pontificia Universidad Catolica de Chile. 5Department of Clinical Laboratories, Pontificia Universidad Catolica de Chile, Santiago, Chile
About 15% of the essential hypertensive patients would have a low activity of the 11βHSD2 enzyme,... more About 15% of the essential hypertensive patients would have a low activity of the 11βHSD2 enzyme, which inactivates cortisol (F) to cortisone (E). Gene expression can be negatively regulated by miRNA. Urinary exosomes and their specific content (miRNA/proteins) represent a valuable tool as a biomarker for the diagnosis and prognosis of the disease. Aim: To evaluate the expression of miRNA specific for 11βHSD2 in samples of urinary exosomes and to determine its association with biochemical variables associated with mineralocorticoid metabolism. Subjects and Methods: Cross-sectional study in subjects between 10-60 years. They were classified into subjects with high F/E (> p75) and low cortisone (< p25) and control subjects. The urinary exosomes were isolated with the Invitrogen kit. Bioinformatic analysis was performed with MirWalk to identify specific miRNAs of HSD11B2. The expression of miRNA was evaluated by qRTPCR. The comparisons were made with the Mann-Whitney test. Result...
Journal of Hypertension, 2019
Objectives: To identify associations among the plasma renin concentration (PRC), plasma aldostero... more Objectives: To identify associations among the plasma renin concentration (PRC), plasma aldosterone and urinary sodium (Na)/potassium (K) ratio, and to integrate these variables into a nomogram with the aim of estimating the expected versus observed aldosterone concentration. Methods: We studied 40 healthy normotensive children (5–8 years old, 57.5% girls) who were born at term and were adequate for their gestational age. Following overnight fasting, the PRC and plasma aldosterone in blood samples were measured, and the Na/K ratio was calculated from a simultaneously obtained urinary spot sample. A mathematical function was defined with these three variables, and a nomogram was built that would return the expected aldosterone concentration from the obtained plasma renin and urinary Na/K ratio values. Results: The PRC (B = 5.9, P < 0.001) and urinary Na/K ratio (B = −98.1, P = 0.003) were significant independent predictors of plasma aldosterone. The correlation between the observ...
American Journal of Hypertension, 2019
BACKGROUND Aldosterone has been linked with obesity, metabolic syndrome (MetS), pro-inflammatory,... more BACKGROUND Aldosterone has been linked with obesity, metabolic syndrome (MetS), pro-inflammatory, and prothrombotic states; however, most studies relate these indicators with primary aldosteronism (PA), excluding non-PA patients. OBJECTIVE To determine whether aldosterone, renin, or the plasma aldosterone/renin ratio (ARR) are associated with metabolic disorders and inflammatory/vascular biomarkers in a non-PA population. METHODS We studied 275 patients including adolescents and adults of both genders and measured plasma and urinary aldosterone and determined the plasma renin activity. In all subjects, the presence of MetS was determined according to Adult Treatment Panel III. Renal, vascular, inflammatory, and mineralocorticoid activity biomarkers were evaluated. RESULTS The ARR correlated with the number of variables of MetS (r = 0.191, P = 0.002), body mass index (BMI; r = 0.136, P = 0.026), systolic blood pressure (r = 0.183, P = 0.002), diastolic blood pressure (r = 0.1917, P =...
The Endocrine Society's 92nd Annual Meeting, June 19–22, 2010 - San Diego, 2010