Marlene Aglony | Pontificia Universidad Catolica de Chile (original) (raw)

Papers by Marlene Aglony

Revista médica de Chile (Impresa), Jan 10, 2011

A cardiovascular risk score for children, that includes traditional risk factors, obesity, sedent... more A cardiovascular risk score for children, that includes traditional risk factors, obesity, sedentary habits and a family history of cardiovascular disease, has been recently proposed by Spanish researchers. To apply this score in school age children in Santiago de Chile and correlate its results with markers of subclinical atherosclerotic disease. Retrospective analysis of data obtained from 209 children, aged 11.5 ± 2 years, studied between 2005 and 2006. Weight, height, blood pressure, ultrasound measurement of carotid intimamedia thickness and fow mediated dilatation of brachial artery and ultrasensible C reactive protein (us PCR) were measured. The Spanish cardiovascular risk score was calculated and correlated with ultrasound parameters and C reactive protein. According to the score, 173 children (83%) had a low cardiovascular risk, 28 (13%) an intermediate risk and 8 (4%) a high risk. There was no association between the cardiovascular risk score and carotid intima-media thick...

Abstract—Familial hyperaldosteronism type 1 is an autosomal dominant disorder attributed to a chi... more Abstract—Familial hyperaldosteronism type 1 is an autosomal dominant disorder attributed to a chimeric CYP11B1/ CYP11B2 gene (CG). Its prevalence and manifestation in the pediatric population has not been established. We aimed to investigate the prevalence of familial hyperaldosteronism type 1 in Chilean hypertensive children and to describe their clinical and biochemical characteristics. We studied 130 untreated hypertensive children (4 to 16 years old). Blood samples for measuring plasma potassium, serum aldosterone, plasma renin activity, aldosterone/renin ratio, and DNA were collected. The detection of CG was performed using long-extension PCR. We found 4 (3.08%) of 130 children with CG who belonged to 4 unrelated families. The 4 patients with CG had very high aldosterone/renin ratio (49 to 242). In addition, we found 4 children and 5 adults who were affected among 21 first-degree relatives. Of the 8 affected children, 6 presented severe hypertension, 1 presented prehypertension...

We reported that 15% of essential hypertensives may suffer from impairment of the enzyme 11beta-h... more We reported that 15% of essential hypertensives may suffer from impairment of the enzyme 11beta-hydroxysteroid-dehydrogenase type 2 (11BHSD2). 11BHSD2 activity and expression can be affected by mutations, polymorphisms, and lately, epigenetic modifications. Aim: To evaluate the HSD11B2 promoter methylation in adults and pediatric essential hyper-tensives (HT). Material and Methods: We recruited 64 patients, grouped in 16 HT and 16 Normotensive (NT) adults; 16 HT and 16 NT pediatrics. We measured serum aldosterone, plasma renin activity (PRA), cortisol (F), cortisone (E) and free urinary cortisol metabolites (THF, aTHF, and THE). PBMC bisulfite–treated DNA was used to perform the methylation-specific PCR (MS-PCR) and calculated the methylation index in HSD11B2 promoter. Results: HT adults have higher methylation index compared with NT adults (0.154±0.031 vs. 0.072±0.011, p<0.05). HT and NT children have low and similar methylation (0.021±0.005 vs. 0.052±0.008, p NS). Methylation i...

DiGeorge syndrome is characterized by developmental defects of the heart, parathyroid glands and ... more DiGeorge syndrome is characterized by developmental defects of the heart, parathyroid glands and thymus. To describe the clinical variability of DiGeorge syndrome and its relation with immunodeficiency. A three years retrospective chart review from three hospitals of Santiago, Chile was conducted. We included patients with neonatal diagnosis of DiGeorge syndrome. Clinical and immunologic data were collected from their initial evaluation. We found 9 patients with DiGeorge syndrome. All had dysmorphic facies, hypocalcemia and congenital heart disease. Three patients had hypoparathyroidism, 4 had interrupted aortic arch type B, 4 had tetralogy of Fallot and 1 had coarctation of aorta. Six patients had other malformations and associated diseases. FISH studies, performed in 8 patients, found the 22q11.2 microdeletion in all. Most patients had low CD3, CD4 and CD8 T cell counts, that ranged for CD3 T cells, between 256/mm3 and 3,664/mm3, for CD4 T cells, between 224/mm3 and 2,649/mm3, for CD8 T cells, between 26/mm3 and 942/mm3. Three patients had CD4 T cells counts &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;400/mm3 and one had a phytohemagglutinin stimulation index &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;10. Airway malformations and primary hypoparathyroidism were present in 3 out of 4 patients that died before 18 months compared with the surviving patents (p=0.048). We found variable clinical manifestations as well as CD3, CD4 and CD8 T cell counts in patients with DiGeorge syndrome. Airway malformations were associated with a higher mortality.

Revista Chilena de Pediatría

La hipertensión arterial (HTA) en niños y adolescentes es una patología importante, asociada a fa... more La hipertensión arterial (HTA) en niños y adolescentes es una patología importante, asociada a factores modificables y no modificables. En la edad pediátrica, la prevalencia de la HTA es de alrededor de un 3,5%, y va aumentando progresivamente con la edad. El método ideal para su diagnóstico es la medición de la presión arterial (PA) con instrumentos auscultatorios. Según lo publicado por la Academia Americana de Pediatría (AAP) la PA debe ser medida en niños mayores de 3 años una vez al año, y en niños menores de 3 años, si presenta factores de riesgo. Una vez confirmada la HTA, la evaluación debe dirigirse hacia la detección de una enfermedad causal y a la búsqueda de factores de riesgo asociados a una HTA primaria. El objetivo del tratamiento de la HTA primaria y secundaria en pediatría es lograr un nivel de PA que disminuya el riesgo de daño de órgano blanco. Las opciones terapéuticas incluyen: tratamiento según etiología específica, no farmacológico y farmacológico. Este docume...

Revista Chilena de Pediatría, Jun 10, 2019

Journal of Hypertension, 2016

Ver información de revista Resumen Objective: To identify novel biomarkers associated with pediat... more Ver información de revista Resumen Objective: To identify novel biomarkers associated with pediatric primary hypertension. Methods: We recruited 350 participants (4-16 years). Anthropometric parameters and aldosterone, plasma renin activity, cortisol, cortisone, Homeostasis Model Assessment Insulin Resistance (HOMA-IR), high-sensitivity C-reactive protein, adiponectin, IL-6, plasminogen activator inhibitor type 1 levels and matrix metalloproteinase-9 and matrix metalloproteinase-2 (MMP-9 and MMP-2) activities were measured. Genomic DNA was isolated. Patients with altered glucose metabolism, severe obesity [BMI-SD score (BMI-SDS)> 2.51, renovascular disease, primary aldosteronism and apparent mineralocorticoid excess syndrome were excluded.

Nutricion Hospitalaria, Apr 1, 2015

Introduction: Endothelial inflammation and insulin resistance (IR) begin in childhood and constit... more Introduction: Endothelial inflammation and insulin resistance (IR) begin in childhood and constitute the pathophysiological basis of Metabolic Syndrome (MS). The increase levels in plasma of inflammatory markers such as high sensitive PCR (hsPCR), plasminogen activator inhibitor 1 (PAI-1) and tests suggestive of IR such as Insulin (Ins) and alanine aminotransferase (ALT) have been associated with MS in adults, but have not been studied in children. Objectives: Correlate the presence of MS and its components with the inflammatory and IR markers seen in the pediatric population. Methods: Cross-sectional study of 337 children (10,9±9,7 years) whose levels of hsPCR, PAI-1, Ins and ALT were determined, along with their association with MS and its individual components. Results: 37 children had MS (10,4%). The frequency of MS components was: abdominal obesity 38,5%, hypertension (HTN) 21,3%, hypertriglyceridemia 17,8%, HDL 21,3% and hyperglycemia 1,4%. hsPCR, PAI-1, ALT and Ins were higher in the presence of MS and increased progressively when components were came together. Conclusions: The pediatric population segment with MS had a higher concentration of hsPCR, PAI-1, Ins and ALT.These levels increase proportionally MS components add up, suggesting that even before diagnosis criteria are fulfilled there is a inflammatory state.

The Endocrine Society's 93rd Annual Meeting & Expo, June 4–7, 2011 - Boston, 2011

Rev Chil Obstet Ginecol, 1999

American Journal of Hypertension, 2016

High sodium intake has been associated with various noncommunicable diseases such as hypertension... more High sodium intake has been associated with various noncommunicable diseases such as hypertension, cardiovascular disease, or stroke; so that reduction of sodium consumption can lower blood pressure and reduce the risk of such noncommunicable diseases. Currently, almost all industrially processed foods contain variable amounts of salt. Therefore, due to the combination of salt added during home food preparation and in industrially processed food, it is difficult to estimate daily salt intake. Defining optimal sodium intake is currently controversial. The American Heart Association (AHA) recommends <1,500 mg/d, 1 the World Health Organization (WHO) recommends <2,000 mg/d for children and adults 2 assuring an appropriate caloric intake in pediatric population and the Institute of Medicine (IOM) indicate that the adequate intake is 1,000-1,500 mg/d according to the age group. 3 In some countries, the sodium intake exceed by far these recommendations. In South Korea, salt intake is 10.5 g

Rev Med Chile, 2007

Many diseases can be associated with kidney cysts and they may be classified as hereditary and no... more Many diseases can be associated with kidney cysts and they may be classified as hereditary and non-hereditary renal cystic disease. The first group can be subclassified as autosomal recessive cystic disease, such as autosomal recessive polycystic kidney disease and nephronophthisis, as autosomal dominant kidney disease such as autosomal dominant polycystic kidney disease, glomerulocystic disease and tuberous sclerosis, and as cysts associated with syndromes. Cystic dysplasia, multicystic dysplastic kidney, simple cyst, multilocular cysts, Wilm's tumor and acquired cystic kidney disease are classified in the second group. The genetic study of renal cysts is becoming increasingly important, due to the possible therapeutic interventions that could be devised in the future. The aim of this review is to provide a fast and easy clinical approach to renal cystc (