Marcelo Moura | Universidade Candido Mendes (original) (raw)

Papers by Marcelo Moura

Animal Reproduction Science, 2008

The present work aimed to evaluate the transcription and replication inhibitor, actinomycin D, fo... more The present work aimed to evaluate the transcription and replication inhibitor, actinomycin D, for oocyte chemical enucleation. Cattle oocytes matured in vitro were treated with actinomycin D according to the following treatments: T1, control; T2 = 1.0 g/ml for 16 h; T3 = 1.0 g/ml for 14 h; T4 = 2.5 g/ml for 14 h; T5 = 5.0 g/ml for 14 h. The oocytes were denuded and activated during 24-26 h of maturation. Oocytes were fixed to determine the maturation status and for chromosome morphology evaluation. Furthermore, oocytes treated with actinomycin D were used for somatic cell nuclear transfer (SCNT). Parthenogenetic and SCNT embryos were fixed to evaluate the percentage of apoptotic nuclei by the TUNEL assay. The maturation (T1 = 90.4%; T2 = 82.3%; T3 = 79.1%; T4 = 83.4%; T5 = 74.7%), cleavage (T1 = 68.9%; T2 = 46.0%; T3 = 49.7%; T4 = 33.4%; T5 = 29.3%) and blastocyst rate at D8 (T1 = 41.1%; T2 = 1.8%; T3 = 1.3%; T4 = 0.9%; T5 = 0.0%) after actinomycin D treatment were significantly different. There was a significant chromosome uncoiling when treated with greater concentrations (2.5 and 5.0 g/ml). After SCNT, the cleavage rate (61.3%) was similar to the actinomycin D-treated control group (61.3%) and less than the non-treated control (70.2%), although the blastocyst rate was greater in the SCNT group (11.8%) comparing with the treated control (3.6%) and less than the untreated control (38.0%). Treated parthenogenetic embryos had more apoptotic cells than the parthenogenetic controls (24.2% compared with 4.8%). However, the SCNT group using treated cytoplasts was similar from the SCNT control (9.3 compared * Corresponding author at: Embrapa Genetics Resources and Biotechnology, W5 Norte Final, 41 with 13.0%). Actinomycin D treatment was efficient in blocking embryonic development. Moreover, it was possible to obtain reconstructed embryos that possess an apoptotic cell index indistinguishable from controls.

... À galera do Colégio de Aplicação do Recife, CAP98, vocês são demais!! ... Ligiane, Dany Perei... more ... À galera do Colégio de Aplicação do Recife, CAP98, vocês são demais!! ... Ligiane, Dany Pereira, Marina, Juliana, Aline, Geórgia, Rafael, Dani Brandão, Natalie, Thiago, Grazielli, José, Sarah, Marcos, Rambinho, Fernanda, Anderson, Anísio, Emivaldo, Tatiane, Ana Cláudia ...

Acta Veterinaria Hungarica, 2015

Exposure of caprine oocytes and embryos to retinoids enhances embryonic development, but the mech... more Exposure of caprine oocytes and embryos to retinoids enhances embryonic development, but the mechanisms governing this phenomenon have not been characterised. The aim of the present study was to evaluate if the incidence of apoptosis is affected by the addition of retinyl acetate (RAc) and 9-cis-retinoic acid (RA) during in vitro maturation (IVM) of caprine oocytes. Embryonic development was recorded on days 3 and 8 post-fertilisation, and apoptosis was measured by caspase activity and DNA fragmentation (TUNEL assay). Control zygotes had lower capacity to cleave and reach the blastocyst stage (24.45 ± 2.32 and 5.32 ± 0.81, respectively) than those of RAc- (29.96 ± 1.62 and 7.94 ± 0.93, respectively) and RA-treated groups (30.12 ± 1.51 and 7.36 ± 1.02, respectively). Oocytes and blastocysts positive for TUNEL assay were more frequent, respectively, in the controls (8.20 ± 0.78, 8.70 ± 1.05) than in RAc (5.60 ± 0.52, 4.80 ± 0.51) and RA (6.40 ± 0.69, 5.40 ± 0.69). Caspase activity did not differ between control oocytes (7.20 ± 0.91), RAc (6.60 ± 0.68) and RA (7.30 ± 0.67), but it was reduced in RAc- (5.05 ± 0.62) and RA-treated blastocysts (5.75 ± 0.22) compared to controls (8.35 ± 0.71). These results indicate that the addition of retinoids during IVM increases the developmental potential of goat embryos with a concomitant reduction in apoptosis rates.

Neste trabalho foi avaliado o efeito do estresse calórico durante a maturação de oócitos sobre a ... more Neste trabalho foi avaliado o efeito do estresse calórico durante a maturação de oócitos sobre a produção in vitro de embriões ovinos. Os ovários foram obtidos em abatedouro e os oócitos colhidos de folículos de 2 a 6 mm de diâmetro. Após seleção, os oócitos, em 10 replicações, foram colocados para maturação in vitro (MIV) durante 24 horas. Os oócitos submetidos ao estresse térmico de 41º C durante 3, 6, 12, 18 e 24 horas foram posteriormente transferidos para completar a MIV a 39 ºC, mesma temperatura utilizada para maturação dos oócitos do grupo controle. O desenvolvimento dos embriões foi determinado nos dias 3, 4, 5 e 8 pós-fecundação. A avaliação da qualidade dos embriões foi efetuada através da contagem total de células coradas pelo DAPI e da determinação do número de blastômeros positivos para apoptose através do teste de TUNEL. Observou-se que o estresse térmico diminuiu (P < 0,05) a capacidade de maturação dos oócitos de acordo com o tempo de exposição à temperatura de 41º C. No grupo de oócitos incubados a 39° C, 70,70% maturou, enquanto que nos grupos expostos ao estresse térmico, apenas 45,28%, 35,17%, 12,30%, 9,74% e 4,60% maturaram, respectivamente, após 3, 6, 12, 18 e 24 horas de incubação. A duração de exposição dos oócitos ao estresse calórico foi inversamente proporcional (P < 0,05) à capacidade de desenvolvimento embrionário e diretamente proporcional (P < 0,05) ao número de blastocistos positivos para apoptose. Todavia, o efeito deletério do estresse térmico sobre a clivagem e os embriões nos estádios de 8 a 16 células e de mórula foi crescente (P > 0,05) somente até 18 horas de incubação. Os resultados permitem concluir que o estresse calórico durante a maturação in vitro de oócitos reduz a quantidade e a qualidade dos embriões ovinos produzidos in vitro determinadas pela alta incidência de apoptose.

Veterinary Science Development, 2014

Ciência Animal Brasileira, 2013

This study aimed to determine the influence of dry and rainy seasons on oocyte maturation and in ... more This study aimed to determine the influence of dry and rainy seasons on oocyte maturation and in vitro production (IVP) of embryos in goats. The ovaries of does in dry (October to March) and rainy season (April-September) were collected at a slaughterhouse and transported to the Laboratory of Biotechnical Reproduction of UFRPE. The cumulus oophorus complexes (COCs) were collected by the technique of

Zygote, 2014

Summary The experiment aimed to compare conventional freezing and different vitrification protoco... more Summary The experiment aimed to compare conventional freezing and different vitrification protocols for cryopreservation of caprine embryos at morphological, ultrastructural, and functional levels. Caprine embryos produced in vivo were allocated randomly to three groups: (1) conventional freezing with ethylene glycol (EG); (2) dimethyl sulfoxide + EG (DMSO/EG) vitrification; and (3) dimethylformamide + EG (DMF/EG) vitrification. All groups were scored for cell viability (propidium iodide staining and ultrastructural levels) and re-expansion rate after thawing or warming. Embryos subjected to DMSO/EG vitrification showed higher cell viability (73.33%), compared with DMF/EG vitrification and conventional freezing group embryos (40.00 and 66.66%, respectively). The ultrastructural study revealed that vitrified embryos had greater preservation of cellular structure than embryos from conventional freezing with EG. DMSO/EG vitrification resulted in higher rates of re-expansion in vitro (47.36%) than DMF/EG vitrification (31.58%), and conventional freezing (25.00%). In conclusion, caprine embryos produced in vivo are better cryopreserved after vitrification than conventional freezing, therefore we conclude that DMSO/EG vitrification is the most effective protocol for cryopreservation.

Animal Reproduction Science, 2008

The present work aimed to evaluate the transcription and replication inhibitor, actinomycin D, fo... more The present work aimed to evaluate the transcription and replication inhibitor, actinomycin D, for oocyte chemical enucleation. Cattle oocytes matured in vitro were treated with actinomycin D according to the following treatments: T1, control; T2 = 1.0 g/ml for 16 h; T3 = 1.0 g/ml for 14 h; T4 = 2.5 g/ml for 14 h; T5 = 5.0 g/ml for 14 h. The oocytes were denuded and activated during 24-26 h of maturation. Oocytes were fixed to determine the maturation status and for chromosome morphology evaluation. Furthermore, oocytes treated with actinomycin D were used for somatic cell nuclear transfer (SCNT). Parthenogenetic and SCNT embryos were fixed to evaluate the percentage of apoptotic nuclei by the TUNEL assay. The maturation (T1 = 90.4%; T2 = 82.3%; T3 = 79.1%; T4 = 83.4%; T5 = 74.7%), cleavage (T1 = 68.9%; T2 = 46.0%; T3 = 49.7%; T4 = 33.4%; T5 = 29.3%) and blastocyst rate at D8 (T1 = 41.1%; T2 = 1.8%; T3 = 1.3%; T4 = 0.9%; T5 = 0.0%) after actinomycin D treatment were significantly different. There was a significant chromosome uncoiling when treated with greater concentrations (2.5 and 5.0 g/ml). After SCNT, the cleavage rate (61.3%) was similar to the actinomycin D-treated control group (61.3%) and less than the non-treated control (70.2%), although the blastocyst rate was greater in the SCNT group (11.8%) comparing with the treated control (3.6%) and less than the untreated control (38.0%). Treated parthenogenetic embryos had more apoptotic cells than the parthenogenetic controls (24.2% compared with 4.8%). However, the SCNT group using treated cytoplasts was similar from the SCNT control (9.3 compared * Corresponding author at: Embrapa Genetics Resources and Biotechnology, W5 Norte Final, 41 with 13.0%). Actinomycin D treatment was efficient in blocking embryonic development. Moreover, it was possible to obtain reconstructed embryos that possess an apoptotic cell index indistinguishable from controls.

Acta Veterinaria Hungarica, 2013

Experiments were carried out to investigate the beneficial effects of retinyl acetate (RAc) and r... more Experiments were carried out to investigate the beneficial effects of retinyl acetate (RAc) and retinoic acid (RA) on goat oocyte maturation as well as the effects of insulin-like growth factor-I (IGF-I), RAc and RA during embryo culture under chemically defined conditions. In Experiment 1, in vitro maturation (IVM) was performed in a chemically defined basic maturation medium (bMM) supplemented with 0.3 μM RAc or 0.5 μM RA. Presumptive zygotes and embryos (2-4 cells) were cultured in droplets of potassium simplex optimised medium (KSOM); however, none of the embryos reached the blastocyst stage. In Experiment 2, oocytes were matured in bMM + RAc or bMM + RA. Presumptive zygotes and 2- to 4-cell embryos were placed in fresh KSOM droplets supplemented with RAc, RA, IGF-I, RAc+IGF-I or RA+IGF-I. In Experiment 1, addition of RAc and RA to bMM increased (P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05) the proportion of 2- to 4-cell embryos reaching the morula stage as compared to the control. In Experiment 2, supplementation of embryo culture media with retinoids and IGF-I increased (P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05) the proportion of 2- to 4-cell stage embryos developing to the morula and blastocyst stage. Our data demonstrate that goat embryo production in chemically defined media could be improved by exogenous RAc or RA and by the interaction between retinoids and IGF-I, and that goat embryos can be produced in vitro from oocytes following protocols similar to those currently used for cattle.

Revista Brasileira De Otorrinolaringologia, 2006

Brazilian journal of otorhinolaryngology

The number of patients with stapes otosclerosis compared to the number of otorhinolaryngologists ... more The number of patients with stapes otosclerosis compared to the number of otorhinolaryngologists has declined over the past several years. As a result a controversy has arisen in the literature, whether or not stapes surgery should be included in residency programs. the objective of the present study is to evaluate the results and complications of estapedotomies performed by residents between January, 1997 and January, 2000, and consequently study the feasibility of including estapedotomies in residency programs. STUD DESIGN: retrospective review of prospectively collected audiometric data. fifty charts of patients that were submitted to a total of 51 primary stapedotomies were reviewed mainly for complications and audiological results. there was closure of the air-bone gap within 10 dB HL in 70.5% of ears and closure to within 20 dB HL in 86.3% of ears. There was one ear with total hearing loss (2%). From the results and complications seen in the present study, and analyzing papers...

Revista Brasileira de Farmacognosia, 2006

RESUMO: "Atividade anti-infl amatória de alkalóides: Uma revisão do século XX". Muitas substância... more RESUMO: "Atividade anti-infl amatória de alkalóides: Uma revisão do século XX". Muitas substâncias que interferem na resposta infl amatória têm sido isoladas de plantas. Esta revisão mostra alguns alcalóides de origem vegetal que no período de 1907-2000 foram avaliados quanto a uma possível atividade anti-infl amatória. Os alcalóides foram classifi cados em subgrupos de acordo com suas estruturas químicas e os dados farmacológicos foram obtidos de diferentes modelos experimentais. Dos 171 alcalóides avaliados, 137 apresentaram atividade anti-infl amatória, e, entre eles, os alcalóides do tipo isoquinolínicos foram os mais estudados. O modelo de edema de pata induzido por carragenina foi o mais empregado para avaliação da atividade anti-infl amatória. Nesta revisão 174 referências foram consultadas.

Revista Brasileira de Otorrinolaringologia, 2006

Resumo/Summary Aproporção de pacientes com otosclerose estapediana em relação ao número de otorri... more Resumo/Summary Aproporção de pacientes com otosclerose estapediana em relação ao número de otorrinolaringologistas tem diminuído nos últimos anos. Questiona-se se a cirurgia de tratamento da otosclerose deve ou não continuar sendo ensinada para ...

Ciência e Agrotecnologia, 2004

Santa Clara&amp;amp;amp;amp;amp;#39; tomato (Lycopersicon esculentum Mill.) plants showing ea... more Santa Clara&amp;amp;amp;amp;amp;#39; tomato (Lycopersicon esculentum Mill.) plants showing earlier leaf senescence and yellowish stigma, fruits with pale yellow when immature and red when reach full ripe stage, associated to a lower rate of ripening and firmer than the wild type, were found in Viçosa, MG. The objective of this study was to evaluate the fruit growth and development of the

Digestive Diseases and Sciences, 1987

The electrical potential difference (PD) of gastric mucosa was measured under direct endoscopic v... more The electrical potential difference (PD) of gastric mucosa was measured under direct endoscopic visualization in 39 patients. Seventy-six observations were made, 39 in fundic mucosa and 37 in the antral region. Biopsies taken from these areas allowed the identification of a control group and a gastritis group. The PD measurements of the control group were higher in the fundic mucosa (P less than 0.001) than in the antral region. In the gastritis group, PD measurements both in the fundic and antral regions were lower than those of the control group (P less than 0.01). However, in the gastritis group, PD measurements of the fundic region were still higher (P less than 0.01) than the PD of the antral region. The correlation coefficient of PD measurements versus the semiquantitative assessment of oxyntic and chief cells of the fundus was r = 0.49 (P less than 0.01).

Conjunto de valores, para os quais o funcional r(U) apresenta um máximo, mínimo ou ponto de sela.

Proceedings of the Seventh International Conference on Computational Structures Technology, 2004

Ciencia e Tecnologia dos Materiais

In this work the mode I fracture properties of bone tissue were determined for the RL propagation... more In this work the mode I fracture properties of bone tissue were determined for the RL propagation system. With this aim, Double Cantilever Beam (DCB) fracture tests were performed using specimens of cortical bone tissue harvested from bovine femurs with an age of approximately 8 months. In order to overcome the difficulty associated to the monitoring of crack length during propagation in the course of the test, the CBBM (Compliance Based Beam Method) was used. This method is based on beam theory and experimental specimen compliance, thus allowing the attainment of an equivalent crack length during the test. The results obtained reveal the existence of a plateau on the Resistance-curve, thus making possible to estimate with accuracy the critical strain energy release rate.

The increasing use of Carbon-Fibre Reinforced Plastic (CFRP) laminates in high responsibility app... more The increasing use of Carbon-Fibre Reinforced Plastic (CFRP) laminates in high responsibility applications introduces an issue regarding their handling after damage. The availability of efficient repair methods is essential to restore the strength of the structure. The availability of accurate predictive tools for the repairs behaviour is also essential for the reduction of costs and time associated to extensive tests. This work reports on a numerical study of the tensile behaviour of three-dimensional (3D) adhesively-bonded scarf repairs in CFRP structures, using a ductile adhesive. The Finite Element (FE) analysis was performed in ABAQUS ® and Cohesive Zone Models (CZM's) was used for the simulation of damage in the adhesive layer. A parametric study was performed on two geometric parameters. The use of over-laminating plies covering the repaired region at the outer or both repair surfaces was also tested as an attempt to increase the repairs efficiency. The results allowed th...

Animal Reproduction Science, 2008

The present work aimed to evaluate the transcription and replication inhibitor, actinomycin D, fo... more The present work aimed to evaluate the transcription and replication inhibitor, actinomycin D, for oocyte chemical enucleation. Cattle oocytes matured in vitro were treated with actinomycin D according to the following treatments: T1, control; T2 = 1.0 g/ml for 16 h; T3 = 1.0 g/ml for 14 h; T4 = 2.5 g/ml for 14 h; T5 = 5.0 g/ml for 14 h. The oocytes were denuded and activated during 24-26 h of maturation. Oocytes were fixed to determine the maturation status and for chromosome morphology evaluation. Furthermore, oocytes treated with actinomycin D were used for somatic cell nuclear transfer (SCNT). Parthenogenetic and SCNT embryos were fixed to evaluate the percentage of apoptotic nuclei by the TUNEL assay. The maturation (T1 = 90.4%; T2 = 82.3%; T3 = 79.1%; T4 = 83.4%; T5 = 74.7%), cleavage (T1 = 68.9%; T2 = 46.0%; T3 = 49.7%; T4 = 33.4%; T5 = 29.3%) and blastocyst rate at D8 (T1 = 41.1%; T2 = 1.8%; T3 = 1.3%; T4 = 0.9%; T5 = 0.0%) after actinomycin D treatment were significantly different. There was a significant chromosome uncoiling when treated with greater concentrations (2.5 and 5.0 g/ml). After SCNT, the cleavage rate (61.3%) was similar to the actinomycin D-treated control group (61.3%) and less than the non-treated control (70.2%), although the blastocyst rate was greater in the SCNT group (11.8%) comparing with the treated control (3.6%) and less than the untreated control (38.0%). Treated parthenogenetic embryos had more apoptotic cells than the parthenogenetic controls (24.2% compared with 4.8%). However, the SCNT group using treated cytoplasts was similar from the SCNT control (9.3 compared * Corresponding author at: Embrapa Genetics Resources and Biotechnology, W5 Norte Final, 41 with 13.0%). Actinomycin D treatment was efficient in blocking embryonic development. Moreover, it was possible to obtain reconstructed embryos that possess an apoptotic cell index indistinguishable from controls.

... À galera do Colégio de Aplicação do Recife, CAP98, vocês são demais!! ... Ligiane, Dany Perei... more ... À galera do Colégio de Aplicação do Recife, CAP98, vocês são demais!! ... Ligiane, Dany Pereira, Marina, Juliana, Aline, Geórgia, Rafael, Dani Brandão, Natalie, Thiago, Grazielli, José, Sarah, Marcos, Rambinho, Fernanda, Anderson, Anísio, Emivaldo, Tatiane, Ana Cláudia ...

Acta Veterinaria Hungarica, 2015

Exposure of caprine oocytes and embryos to retinoids enhances embryonic development, but the mech... more Exposure of caprine oocytes and embryos to retinoids enhances embryonic development, but the mechanisms governing this phenomenon have not been characterised. The aim of the present study was to evaluate if the incidence of apoptosis is affected by the addition of retinyl acetate (RAc) and 9-cis-retinoic acid (RA) during in vitro maturation (IVM) of caprine oocytes. Embryonic development was recorded on days 3 and 8 post-fertilisation, and apoptosis was measured by caspase activity and DNA fragmentation (TUNEL assay). Control zygotes had lower capacity to cleave and reach the blastocyst stage (24.45 ± 2.32 and 5.32 ± 0.81, respectively) than those of RAc- (29.96 ± 1.62 and 7.94 ± 0.93, respectively) and RA-treated groups (30.12 ± 1.51 and 7.36 ± 1.02, respectively). Oocytes and blastocysts positive for TUNEL assay were more frequent, respectively, in the controls (8.20 ± 0.78, 8.70 ± 1.05) than in RAc (5.60 ± 0.52, 4.80 ± 0.51) and RA (6.40 ± 0.69, 5.40 ± 0.69). Caspase activity did not differ between control oocytes (7.20 ± 0.91), RAc (6.60 ± 0.68) and RA (7.30 ± 0.67), but it was reduced in RAc- (5.05 ± 0.62) and RA-treated blastocysts (5.75 ± 0.22) compared to controls (8.35 ± 0.71). These results indicate that the addition of retinoids during IVM increases the developmental potential of goat embryos with a concomitant reduction in apoptosis rates.

Neste trabalho foi avaliado o efeito do estresse calórico durante a maturação de oócitos sobre a ... more Neste trabalho foi avaliado o efeito do estresse calórico durante a maturação de oócitos sobre a produção in vitro de embriões ovinos. Os ovários foram obtidos em abatedouro e os oócitos colhidos de folículos de 2 a 6 mm de diâmetro. Após seleção, os oócitos, em 10 replicações, foram colocados para maturação in vitro (MIV) durante 24 horas. Os oócitos submetidos ao estresse térmico de 41º C durante 3, 6, 12, 18 e 24 horas foram posteriormente transferidos para completar a MIV a 39 ºC, mesma temperatura utilizada para maturação dos oócitos do grupo controle. O desenvolvimento dos embriões foi determinado nos dias 3, 4, 5 e 8 pós-fecundação. A avaliação da qualidade dos embriões foi efetuada através da contagem total de células coradas pelo DAPI e da determinação do número de blastômeros positivos para apoptose através do teste de TUNEL. Observou-se que o estresse térmico diminuiu (P < 0,05) a capacidade de maturação dos oócitos de acordo com o tempo de exposição à temperatura de 41º C. No grupo de oócitos incubados a 39° C, 70,70% maturou, enquanto que nos grupos expostos ao estresse térmico, apenas 45,28%, 35,17%, 12,30%, 9,74% e 4,60% maturaram, respectivamente, após 3, 6, 12, 18 e 24 horas de incubação. A duração de exposição dos oócitos ao estresse calórico foi inversamente proporcional (P < 0,05) à capacidade de desenvolvimento embrionário e diretamente proporcional (P < 0,05) ao número de blastocistos positivos para apoptose. Todavia, o efeito deletério do estresse térmico sobre a clivagem e os embriões nos estádios de 8 a 16 células e de mórula foi crescente (P > 0,05) somente até 18 horas de incubação. Os resultados permitem concluir que o estresse calórico durante a maturação in vitro de oócitos reduz a quantidade e a qualidade dos embriões ovinos produzidos in vitro determinadas pela alta incidência de apoptose.

Veterinary Science Development, 2014

Ciência Animal Brasileira, 2013

This study aimed to determine the influence of dry and rainy seasons on oocyte maturation and in ... more This study aimed to determine the influence of dry and rainy seasons on oocyte maturation and in vitro production (IVP) of embryos in goats. The ovaries of does in dry (October to March) and rainy season (April-September) were collected at a slaughterhouse and transported to the Laboratory of Biotechnical Reproduction of UFRPE. The cumulus oophorus complexes (COCs) were collected by the technique of

Zygote, 2014

Summary The experiment aimed to compare conventional freezing and different vitrification protoco... more Summary The experiment aimed to compare conventional freezing and different vitrification protocols for cryopreservation of caprine embryos at morphological, ultrastructural, and functional levels. Caprine embryos produced in vivo were allocated randomly to three groups: (1) conventional freezing with ethylene glycol (EG); (2) dimethyl sulfoxide + EG (DMSO/EG) vitrification; and (3) dimethylformamide + EG (DMF/EG) vitrification. All groups were scored for cell viability (propidium iodide staining and ultrastructural levels) and re-expansion rate after thawing or warming. Embryos subjected to DMSO/EG vitrification showed higher cell viability (73.33%), compared with DMF/EG vitrification and conventional freezing group embryos (40.00 and 66.66%, respectively). The ultrastructural study revealed that vitrified embryos had greater preservation of cellular structure than embryos from conventional freezing with EG. DMSO/EG vitrification resulted in higher rates of re-expansion in vitro (47.36%) than DMF/EG vitrification (31.58%), and conventional freezing (25.00%). In conclusion, caprine embryos produced in vivo are better cryopreserved after vitrification than conventional freezing, therefore we conclude that DMSO/EG vitrification is the most effective protocol for cryopreservation.

Animal Reproduction Science, 2008

The present work aimed to evaluate the transcription and replication inhibitor, actinomycin D, fo... more The present work aimed to evaluate the transcription and replication inhibitor, actinomycin D, for oocyte chemical enucleation. Cattle oocytes matured in vitro were treated with actinomycin D according to the following treatments: T1, control; T2 = 1.0 g/ml for 16 h; T3 = 1.0 g/ml for 14 h; T4 = 2.5 g/ml for 14 h; T5 = 5.0 g/ml for 14 h. The oocytes were denuded and activated during 24-26 h of maturation. Oocytes were fixed to determine the maturation status and for chromosome morphology evaluation. Furthermore, oocytes treated with actinomycin D were used for somatic cell nuclear transfer (SCNT). Parthenogenetic and SCNT embryos were fixed to evaluate the percentage of apoptotic nuclei by the TUNEL assay. The maturation (T1 = 90.4%; T2 = 82.3%; T3 = 79.1%; T4 = 83.4%; T5 = 74.7%), cleavage (T1 = 68.9%; T2 = 46.0%; T3 = 49.7%; T4 = 33.4%; T5 = 29.3%) and blastocyst rate at D8 (T1 = 41.1%; T2 = 1.8%; T3 = 1.3%; T4 = 0.9%; T5 = 0.0%) after actinomycin D treatment were significantly different. There was a significant chromosome uncoiling when treated with greater concentrations (2.5 and 5.0 g/ml). After SCNT, the cleavage rate (61.3%) was similar to the actinomycin D-treated control group (61.3%) and less than the non-treated control (70.2%), although the blastocyst rate was greater in the SCNT group (11.8%) comparing with the treated control (3.6%) and less than the untreated control (38.0%). Treated parthenogenetic embryos had more apoptotic cells than the parthenogenetic controls (24.2% compared with 4.8%). However, the SCNT group using treated cytoplasts was similar from the SCNT control (9.3 compared * Corresponding author at: Embrapa Genetics Resources and Biotechnology, W5 Norte Final, 41 with 13.0%). Actinomycin D treatment was efficient in blocking embryonic development. Moreover, it was possible to obtain reconstructed embryos that possess an apoptotic cell index indistinguishable from controls.

Acta Veterinaria Hungarica, 2013

Experiments were carried out to investigate the beneficial effects of retinyl acetate (RAc) and r... more Experiments were carried out to investigate the beneficial effects of retinyl acetate (RAc) and retinoic acid (RA) on goat oocyte maturation as well as the effects of insulin-like growth factor-I (IGF-I), RAc and RA during embryo culture under chemically defined conditions. In Experiment 1, in vitro maturation (IVM) was performed in a chemically defined basic maturation medium (bMM) supplemented with 0.3 μM RAc or 0.5 μM RA. Presumptive zygotes and embryos (2-4 cells) were cultured in droplets of potassium simplex optimised medium (KSOM); however, none of the embryos reached the blastocyst stage. In Experiment 2, oocytes were matured in bMM + RAc or bMM + RA. Presumptive zygotes and 2- to 4-cell embryos were placed in fresh KSOM droplets supplemented with RAc, RA, IGF-I, RAc+IGF-I or RA+IGF-I. In Experiment 1, addition of RAc and RA to bMM increased (P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05) the proportion of 2- to 4-cell embryos reaching the morula stage as compared to the control. In Experiment 2, supplementation of embryo culture media with retinoids and IGF-I increased (P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05) the proportion of 2- to 4-cell stage embryos developing to the morula and blastocyst stage. Our data demonstrate that goat embryo production in chemically defined media could be improved by exogenous RAc or RA and by the interaction between retinoids and IGF-I, and that goat embryos can be produced in vitro from oocytes following protocols similar to those currently used for cattle.

Revista Brasileira De Otorrinolaringologia, 2006

Brazilian journal of otorhinolaryngology

The number of patients with stapes otosclerosis compared to the number of otorhinolaryngologists ... more The number of patients with stapes otosclerosis compared to the number of otorhinolaryngologists has declined over the past several years. As a result a controversy has arisen in the literature, whether or not stapes surgery should be included in residency programs. the objective of the present study is to evaluate the results and complications of estapedotomies performed by residents between January, 1997 and January, 2000, and consequently study the feasibility of including estapedotomies in residency programs. STUD DESIGN: retrospective review of prospectively collected audiometric data. fifty charts of patients that were submitted to a total of 51 primary stapedotomies were reviewed mainly for complications and audiological results. there was closure of the air-bone gap within 10 dB HL in 70.5% of ears and closure to within 20 dB HL in 86.3% of ears. There was one ear with total hearing loss (2%). From the results and complications seen in the present study, and analyzing papers...

Revista Brasileira de Farmacognosia, 2006

RESUMO: "Atividade anti-infl amatória de alkalóides: Uma revisão do século XX". Muitas substância... more RESUMO: "Atividade anti-infl amatória de alkalóides: Uma revisão do século XX". Muitas substâncias que interferem na resposta infl amatória têm sido isoladas de plantas. Esta revisão mostra alguns alcalóides de origem vegetal que no período de 1907-2000 foram avaliados quanto a uma possível atividade anti-infl amatória. Os alcalóides foram classifi cados em subgrupos de acordo com suas estruturas químicas e os dados farmacológicos foram obtidos de diferentes modelos experimentais. Dos 171 alcalóides avaliados, 137 apresentaram atividade anti-infl amatória, e, entre eles, os alcalóides do tipo isoquinolínicos foram os mais estudados. O modelo de edema de pata induzido por carragenina foi o mais empregado para avaliação da atividade anti-infl amatória. Nesta revisão 174 referências foram consultadas.

Revista Brasileira de Otorrinolaringologia, 2006

Resumo/Summary Aproporção de pacientes com otosclerose estapediana em relação ao número de otorri... more Resumo/Summary Aproporção de pacientes com otosclerose estapediana em relação ao número de otorrinolaringologistas tem diminuído nos últimos anos. Questiona-se se a cirurgia de tratamento da otosclerose deve ou não continuar sendo ensinada para ...

Ciência e Agrotecnologia, 2004

Santa Clara&amp;amp;amp;amp;amp;#39; tomato (Lycopersicon esculentum Mill.) plants showing ea... more Santa Clara&amp;amp;amp;amp;amp;#39; tomato (Lycopersicon esculentum Mill.) plants showing earlier leaf senescence and yellowish stigma, fruits with pale yellow when immature and red when reach full ripe stage, associated to a lower rate of ripening and firmer than the wild type, were found in Viçosa, MG. The objective of this study was to evaluate the fruit growth and development of the

Digestive Diseases and Sciences, 1987

The electrical potential difference (PD) of gastric mucosa was measured under direct endoscopic v... more The electrical potential difference (PD) of gastric mucosa was measured under direct endoscopic visualization in 39 patients. Seventy-six observations were made, 39 in fundic mucosa and 37 in the antral region. Biopsies taken from these areas allowed the identification of a control group and a gastritis group. The PD measurements of the control group were higher in the fundic mucosa (P less than 0.001) than in the antral region. In the gastritis group, PD measurements both in the fundic and antral regions were lower than those of the control group (P less than 0.01). However, in the gastritis group, PD measurements of the fundic region were still higher (P less than 0.01) than the PD of the antral region. The correlation coefficient of PD measurements versus the semiquantitative assessment of oxyntic and chief cells of the fundus was r = 0.49 (P less than 0.01).

Conjunto de valores, para os quais o funcional r(U) apresenta um máximo, mínimo ou ponto de sela.

Proceedings of the Seventh International Conference on Computational Structures Technology, 2004

Ciencia e Tecnologia dos Materiais

In this work the mode I fracture properties of bone tissue were determined for the RL propagation... more In this work the mode I fracture properties of bone tissue were determined for the RL propagation system. With this aim, Double Cantilever Beam (DCB) fracture tests were performed using specimens of cortical bone tissue harvested from bovine femurs with an age of approximately 8 months. In order to overcome the difficulty associated to the monitoring of crack length during propagation in the course of the test, the CBBM (Compliance Based Beam Method) was used. This method is based on beam theory and experimental specimen compliance, thus allowing the attainment of an equivalent crack length during the test. The results obtained reveal the existence of a plateau on the Resistance-curve, thus making possible to estimate with accuracy the critical strain energy release rate.

The increasing use of Carbon-Fibre Reinforced Plastic (CFRP) laminates in high responsibility app... more The increasing use of Carbon-Fibre Reinforced Plastic (CFRP) laminates in high responsibility applications introduces an issue regarding their handling after damage. The availability of efficient repair methods is essential to restore the strength of the structure. The availability of accurate predictive tools for the repairs behaviour is also essential for the reduction of costs and time associated to extensive tests. This work reports on a numerical study of the tensile behaviour of three-dimensional (3D) adhesively-bonded scarf repairs in CFRP structures, using a ductile adhesive. The Finite Element (FE) analysis was performed in ABAQUS ® and Cohesive Zone Models (CZM's) was used for the simulation of damage in the adhesive layer. A parametric study was performed on two geometric parameters. The use of over-laminating plies covering the repaired region at the outer or both repair surfaces was also tested as an attempt to increase the repairs efficiency. The results allowed th...