Jian Wu | University of California, Davis (original) (raw)
Papers by Jian Wu
Proceedings of the American Society for Information Science and Technology, 2006
In state government web archiving, new websites of required by statute to be archived are continu... more In state government web archiving, new websites of required by statute to be archived are continually discovered. Largely manual techniques used to date to locate new state websites are here supplemented with automated hyperlink extraction. The rate of discovering new state websites quadrupled, at the cost of having to analyze thousands of hyperlinks. Newly discovered websites tended to be smaller, and often concerned adjunct efforts of agencies for which some other website(s) were already known. Beginning the website discovery process using a combination of what is known, or easily discovered manually, followed by the more thorough analysis of hyperlinks seems likely to discover the largest percentage of state government websites most economically.
Materials Science Forum, 2008
Clinical & Translational Immunology, 2015
The competence of cellular immunity depends on a diverse T-cell receptor (TCR) repertoire arising... more The competence of cellular immunity depends on a diverse T-cell receptor (TCR) repertoire arising from thymic output. Normal thymopoiesis arises from marrow-derived CD3 − CD4 − CD8 − triple-negative T-cell progenitors (TN), which develop into mature single-positive (SP) CD4 or CD8 T cells after expressing both CD4 and CD8 (double-positive, DP) transiently, leading to de novo T-cell production. Interleukin-7 (IL7) is a singularly important common γ-chain IL involved in normal thymic development.
Key Engineering Materials, 2007
... Jana and Ong [3] did the ... [1] T.Maeda: J. Soc. Prec.Mech. Japan Vol.25 (1959), p.248 [2] T... more ... Jana and Ong [3] did the ... [1] T.Maeda: J. Soc. Prec.Mech. Japan Vol.25 (1959), p.248 [2] T.Jimma: Bulletin of the JSME Vol.6 (1963), p.568 [3] S.Jana, NSOng: J.Mech Working Tech. Vol.19 (1978), p.55 [4] E.Taupin,J.Breitling,WTWu and T.Altan: J. Mater. Process. Tech. ...
Pest management science, Jan 6, 2015
1,4-Pentadien-3-one and 1,3,4-oxadiazole derivatives possess good antiviral activities, their sub... more 1,4-Pentadien-3-one and 1,3,4-oxadiazole derivatives possess good antiviral activities, their substructure units are usually used in antiviral agent design. In order to discover novel molecules with high antiviral activities, a series of 1,4-pentadien-3-one derivatives containing the 1,3,4-oxadiazole moiety were designed and synthesized. Bioassays showed that most of the title compounds exhibited good inhibitory activities against tobacco mosaic virus (TMV) in vivo. In particular, compound 8f possessed the best protective activity against TMV, with EC50 value of 135.56 mg L(-1) , which were superior to ribavirin (435.99 mg L(-1) ). The comparative molecular field analysis (CoMFA) and comparative molecular similarity index analysis (CoMSIA) of 3D-QSAR with respect to the protective activities were studied with the values of q(2) and r(2) for CoMFA and CoMSIA model were 0.751, 0.775 and 0.936, 0.925 respectively. Compound 8 k was designed and synthesized based on the 3D-QSAR model, an...
Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society, Jan 30, 2015
Liver grafts from donation after cardiac death (DCD) are associated with inferior clinical outcom... more Liver grafts from donation after cardiac death (DCD) are associated with inferior clinical outcome and a high discard rate because of poor graft quality. We investigated the predictive value of DCD liver biopsy in pre-transplant graft quality evaluation. DCD liver transplants which took place between October 2010 and April 2014 were included (n = 127). Histological features of graft biopsy were analyzed to assess risk factors for graft survival. Macrovesicular steatosis ≥ 20% (Hazard ratio [HR] = 2.973, P = 0.045) and sinusoidal neutrophilic infiltrate (HR = 6.969, P = 0.005) were confirmed as independent risk factors for graft survival; hepatocellular swelling, vacuolation and necrosis failed to show prognostic value. Additionally, donor serum total bilirubin ≥ 34.2 μmol/L was also associated with a lower probability of graft survival. Our analysis indicates that macrovesicular steatosis ≥ 20% and sinusoidal neutrophilic infiltrate are novel and useful histological markers for DCD ...
Genetic engineering of tumor cells to express both granulocyte-mac- rophage colony-stimulating fa... more Genetic engineering of tumor cells to express both granulocyte-mac- rophage colony-stimulating factor (GM-CSF) and interleukin (IL)-2 can induce synergistic immune antitumor effects. Paradoxically, the combina- tion has also been reported to down-regulate certain immune functions, highlighting the unpredictability of dual cytokine use. We hypothesized that a GM-CSF and IL-2 fusion transgene (GIFT) could circumvent such limitations yet preserve synergistic features.
Cancer research, 2014
Engineered chimeric cytokines can generate gain-of-function activity in immune cells. Here, we re... more Engineered chimeric cytokines can generate gain-of-function activity in immune cells. Here, we report potent antitumor activity for a novel fusion cytokine generated by N-terminal coupling of GM-CSF to IL4, generating a fusokine termed GIFT4. B cells treated with GIFT4 clustered GM-CSF and IL4 receptors on the cell surface and displayed a pan-STAT hyperphosphorylation associated with acquisition of a distinct phenotype and function described to date. In C57BL/6J mice, administration of GIFT4 expanded endogenous B cells and suppressed the growth of B16F0 melanoma cells. Furthermore, B16F0 melanoma cells engineered to secrete GIFT4 were rejected immunologically in a B-cell-dependent manner. This effect was abolished when GIFT4-expressing B16F0 cells were implanted in B-cell-deficient mice, confirming a B-cell-dependent antitumor effect. Human GIFT4-licensed B cells primed cytotoxic T cells and specifically killed melanoma cells in vitro and in vivo. Taken together, our results demonst...
Key Engineering Materials, 2008
Key Engineering Materials, 2006
ABSTRACT
Theoretical and Applied Genetics, 2012
We developed two mutant populations of oilseed rape (Brassica napus L.) using EMS (ethylmethanesu... more We developed two mutant populations of oilseed rape (Brassica napus L.) using EMS (ethylmethanesulfonate) as a mutagen. The populations were derived from the spring type line YN01-429 and the winter type cultivar Express 617 encompassing 5,361 and 3,488 M 2 plants, respectively. A high-throughput screening protocol was established based on a two-dimensional 89 pooling strategy. Genes of the sinapine biosynthesis pathway were chosen for determining the mutation frequencies and for creating novel genetic variation for rapeseed breeding. The extraction meal of oilseed rape is a rich protein source containing about 40% protein. Its use as an animal feed or human food, however, is limited by antinutritive compounds like sinapine. The targeting induced local lesions in genomes (TILLING) strategy was applied to identify mutations of major genes of the sinapine biosynthesis pathway. We constructed locus-specific primers for several TILLING amplicons of two sinapine synthesis genes, BnaX.SGT and BnaX.REF1, covering 80-90% of the coding sequences. Screening of both populations revealed 229 and 341 mutations within the BnaX.SGT sequences (135 missense and 13 nonsense mutations) and the BnaX.REF1 sequences (162 missense, 3 nonsense, 8 splice site mutations), respectively. These mutants provide a new resource for breeding low-sinapine oilseed rape. The frequencies of missense and nonsense mutations corresponded to the frequencies of the target codons. Mutation frequencies ranged from 1/12 to 1/22 kb for the Express 617 population and from 1/27 to 1/60 kb for the YN01-429 population. Our TILLING resource is publicly available. Due to the high mutation frequencies in combination with an 89 pooling strategy, mutants can be routinely identified in a cost-efficient manner. However, primers have to be carefully designed to amplify single sequences from the polyploid rapeseed genome. Communicated by R. Visser. Hans-Joachim Harloff and Susanne Lemcke have equally contributed. Electronic supplementary material The online version of this article (
Human Mutation, 2004
Recent structural studies of the ligand-binding domain (LBD) of the androgen receptor (AR) have r... more Recent structural studies of the ligand-binding domain (LBD) of the androgen receptor (AR) have raised more questions than answers, as most of the known pathogenic mutations of the AR gene causing androgen insensitivity syndrome (AIS) are not in the ligand-binding pocket. In this study, we have investigated one such pathogenic mutation, by examining details of its altered atomic structure using a computational technique of molecular dynamics (MD) simulations extended over 4 ns, effectively creating a 4D structural model. The mutation R774C, which is in the LBD of the AR gene, causes complete AIS (CAIS), producing ARs that have a unique thermolabile profile, being thermostable at 221C but thermolabile at 371C. We have therefore investigated this mutation by MD simulations at 293 K (201C), 300 K (271C), and 310 K (371C). The MD simulations indicate that: 1) the mutation causes local structural distortions, which result in changes in the shape of the ligand-binding pocket; 2) the mutation alters the dynamic nature of the protein and results in a more diverse conformational distribution of the ligand-binding pocket; and 3) the effect of the mutation on AR structure could be largely reversed by lowering the temperature at which the MD simulations were conducted. These results therefore strongly support the biochemical data, e.g., the mutants' inability to form AR-ligand complexes at 371C and its characteristic reversible thermolability, clearly indicating the value of such computational methods. Hum Mutat 22:465-475,
Human Mutation, 2004
The current version of the androgen receptor (AR) gene mutations database is described. The total... more The current version of the androgen receptor (AR) gene mutations database is described. The total number of reported mutations has risen from 374 to 605, and the number of AR-interacting proteins described has increased from 23 to 70, both over the past 3 years. A 3D model of the AR ligand-binding domain (AR LBD) has been added to give a better understanding of gene structure-function relationships. In addition, silent mutations have now been reported in both androgen insensitivity syndrome (AIS) and prostate cancer (CaP) cases. The database also now incorporates information on the exon 1 CAG repeat expansion disease, spinobulbar muscular atrophy (SBMA), as well as CAG repeat length variations associated with risk for female breast, uterine endometrial, colorectal, and prostate cancer, as well as for male infertility. The possible implications of somatic mutations, as opposed to germline mutations, in the development of future locus-specific mutation databases (LSDBs) is discussed. The database is available on the Internet (http://www.mcgill.ca/androgendb/).
Liver Transplantation, 2006
BACKGROUND: Hepatic ischemia/reperfusion (I/R) injury is characterized by the generation of react... more BACKGROUND: Hepatic ischemia/reperfusion (I/R) injury is characterized by the generation of reactive oxygen species (ROS), such as superoxide anions and hydrogen peroxide. The aim of this study is to investigate whether anti-oxidative gene delivery by our polylipid nanoparticles (PLNP) is an effective approach for prevention of the injury. METHODS: Polyplexes of extracellular superoxide dismutase (EC-SOD) and/or catalase genes were injected via the portal vein one day prior to a warm I/R procedure in mice. The effects of the gene delivery were determined 6 hours after starting reperfusion. RESULTS: PLNP-mediated anti-oxidative gene delivery led to a marked increase in human EC-SOD and catalase gene expression in the liver. Liver SOD and catalase activity both increased approximately 10 fold. Increased liver superoxide anion levels caused by the I/R procedure were reduced to normal levels by EC-SOD gene delivery. The overexpression of these two anti-oxidative genes significantly suppressed the I/R-induced elevation of serum alanine aminotransferase levels, decreased liver malondialdehyde content, restored glutathione reserve, and improved liver histology. CONCLUSION: EC-SOD or catalase gene delivery by PLNP resulted in high levels of the transgene activity in the liver, and markedly attenuated hepatic I/R injury. The protection is directly associated with elevated anti-oxidative enzyme activity as the result of the gene delivery. This novel approach may become a potential therapy to improve graft function and survival after liver transplantation.
Proceedings of the American Society for Information Science and Technology, 2006
In state government web archiving, new websites of required by statute to be archived are continu... more In state government web archiving, new websites of required by statute to be archived are continually discovered. Largely manual techniques used to date to locate new state websites are here supplemented with automated hyperlink extraction. The rate of discovering new state websites quadrupled, at the cost of having to analyze thousands of hyperlinks. Newly discovered websites tended to be smaller, and often concerned adjunct efforts of agencies for which some other website(s) were already known. Beginning the website discovery process using a combination of what is known, or easily discovered manually, followed by the more thorough analysis of hyperlinks seems likely to discover the largest percentage of state government websites most economically.
Materials Science Forum, 2008
Clinical & Translational Immunology, 2015
The competence of cellular immunity depends on a diverse T-cell receptor (TCR) repertoire arising... more The competence of cellular immunity depends on a diverse T-cell receptor (TCR) repertoire arising from thymic output. Normal thymopoiesis arises from marrow-derived CD3 − CD4 − CD8 − triple-negative T-cell progenitors (TN), which develop into mature single-positive (SP) CD4 or CD8 T cells after expressing both CD4 and CD8 (double-positive, DP) transiently, leading to de novo T-cell production. Interleukin-7 (IL7) is a singularly important common γ-chain IL involved in normal thymic development.
Key Engineering Materials, 2007
... Jana and Ong [3] did the ... [1] T.Maeda: J. Soc. Prec.Mech. Japan Vol.25 (1959), p.248 [2] T... more ... Jana and Ong [3] did the ... [1] T.Maeda: J. Soc. Prec.Mech. Japan Vol.25 (1959), p.248 [2] T.Jimma: Bulletin of the JSME Vol.6 (1963), p.568 [3] S.Jana, NSOng: J.Mech Working Tech. Vol.19 (1978), p.55 [4] E.Taupin,J.Breitling,WTWu and T.Altan: J. Mater. Process. Tech. ...
Pest management science, Jan 6, 2015
1,4-Pentadien-3-one and 1,3,4-oxadiazole derivatives possess good antiviral activities, their sub... more 1,4-Pentadien-3-one and 1,3,4-oxadiazole derivatives possess good antiviral activities, their substructure units are usually used in antiviral agent design. In order to discover novel molecules with high antiviral activities, a series of 1,4-pentadien-3-one derivatives containing the 1,3,4-oxadiazole moiety were designed and synthesized. Bioassays showed that most of the title compounds exhibited good inhibitory activities against tobacco mosaic virus (TMV) in vivo. In particular, compound 8f possessed the best protective activity against TMV, with EC50 value of 135.56 mg L(-1) , which were superior to ribavirin (435.99 mg L(-1) ). The comparative molecular field analysis (CoMFA) and comparative molecular similarity index analysis (CoMSIA) of 3D-QSAR with respect to the protective activities were studied with the values of q(2) and r(2) for CoMFA and CoMSIA model were 0.751, 0.775 and 0.936, 0.925 respectively. Compound 8 k was designed and synthesized based on the 3D-QSAR model, an...
Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society, Jan 30, 2015
Liver grafts from donation after cardiac death (DCD) are associated with inferior clinical outcom... more Liver grafts from donation after cardiac death (DCD) are associated with inferior clinical outcome and a high discard rate because of poor graft quality. We investigated the predictive value of DCD liver biopsy in pre-transplant graft quality evaluation. DCD liver transplants which took place between October 2010 and April 2014 were included (n = 127). Histological features of graft biopsy were analyzed to assess risk factors for graft survival. Macrovesicular steatosis ≥ 20% (Hazard ratio [HR] = 2.973, P = 0.045) and sinusoidal neutrophilic infiltrate (HR = 6.969, P = 0.005) were confirmed as independent risk factors for graft survival; hepatocellular swelling, vacuolation and necrosis failed to show prognostic value. Additionally, donor serum total bilirubin ≥ 34.2 μmol/L was also associated with a lower probability of graft survival. Our analysis indicates that macrovesicular steatosis ≥ 20% and sinusoidal neutrophilic infiltrate are novel and useful histological markers for DCD ...
Genetic engineering of tumor cells to express both granulocyte-mac- rophage colony-stimulating fa... more Genetic engineering of tumor cells to express both granulocyte-mac- rophage colony-stimulating factor (GM-CSF) and interleukin (IL)-2 can induce synergistic immune antitumor effects. Paradoxically, the combina- tion has also been reported to down-regulate certain immune functions, highlighting the unpredictability of dual cytokine use. We hypothesized that a GM-CSF and IL-2 fusion transgene (GIFT) could circumvent such limitations yet preserve synergistic features.
Cancer research, 2014
Engineered chimeric cytokines can generate gain-of-function activity in immune cells. Here, we re... more Engineered chimeric cytokines can generate gain-of-function activity in immune cells. Here, we report potent antitumor activity for a novel fusion cytokine generated by N-terminal coupling of GM-CSF to IL4, generating a fusokine termed GIFT4. B cells treated with GIFT4 clustered GM-CSF and IL4 receptors on the cell surface and displayed a pan-STAT hyperphosphorylation associated with acquisition of a distinct phenotype and function described to date. In C57BL/6J mice, administration of GIFT4 expanded endogenous B cells and suppressed the growth of B16F0 melanoma cells. Furthermore, B16F0 melanoma cells engineered to secrete GIFT4 were rejected immunologically in a B-cell-dependent manner. This effect was abolished when GIFT4-expressing B16F0 cells were implanted in B-cell-deficient mice, confirming a B-cell-dependent antitumor effect. Human GIFT4-licensed B cells primed cytotoxic T cells and specifically killed melanoma cells in vitro and in vivo. Taken together, our results demonst...
Key Engineering Materials, 2008
Key Engineering Materials, 2006
ABSTRACT
Theoretical and Applied Genetics, 2012
We developed two mutant populations of oilseed rape (Brassica napus L.) using EMS (ethylmethanesu... more We developed two mutant populations of oilseed rape (Brassica napus L.) using EMS (ethylmethanesulfonate) as a mutagen. The populations were derived from the spring type line YN01-429 and the winter type cultivar Express 617 encompassing 5,361 and 3,488 M 2 plants, respectively. A high-throughput screening protocol was established based on a two-dimensional 89 pooling strategy. Genes of the sinapine biosynthesis pathway were chosen for determining the mutation frequencies and for creating novel genetic variation for rapeseed breeding. The extraction meal of oilseed rape is a rich protein source containing about 40% protein. Its use as an animal feed or human food, however, is limited by antinutritive compounds like sinapine. The targeting induced local lesions in genomes (TILLING) strategy was applied to identify mutations of major genes of the sinapine biosynthesis pathway. We constructed locus-specific primers for several TILLING amplicons of two sinapine synthesis genes, BnaX.SGT and BnaX.REF1, covering 80-90% of the coding sequences. Screening of both populations revealed 229 and 341 mutations within the BnaX.SGT sequences (135 missense and 13 nonsense mutations) and the BnaX.REF1 sequences (162 missense, 3 nonsense, 8 splice site mutations), respectively. These mutants provide a new resource for breeding low-sinapine oilseed rape. The frequencies of missense and nonsense mutations corresponded to the frequencies of the target codons. Mutation frequencies ranged from 1/12 to 1/22 kb for the Express 617 population and from 1/27 to 1/60 kb for the YN01-429 population. Our TILLING resource is publicly available. Due to the high mutation frequencies in combination with an 89 pooling strategy, mutants can be routinely identified in a cost-efficient manner. However, primers have to be carefully designed to amplify single sequences from the polyploid rapeseed genome. Communicated by R. Visser. Hans-Joachim Harloff and Susanne Lemcke have equally contributed. Electronic supplementary material The online version of this article (
Human Mutation, 2004
Recent structural studies of the ligand-binding domain (LBD) of the androgen receptor (AR) have r... more Recent structural studies of the ligand-binding domain (LBD) of the androgen receptor (AR) have raised more questions than answers, as most of the known pathogenic mutations of the AR gene causing androgen insensitivity syndrome (AIS) are not in the ligand-binding pocket. In this study, we have investigated one such pathogenic mutation, by examining details of its altered atomic structure using a computational technique of molecular dynamics (MD) simulations extended over 4 ns, effectively creating a 4D structural model. The mutation R774C, which is in the LBD of the AR gene, causes complete AIS (CAIS), producing ARs that have a unique thermolabile profile, being thermostable at 221C but thermolabile at 371C. We have therefore investigated this mutation by MD simulations at 293 K (201C), 300 K (271C), and 310 K (371C). The MD simulations indicate that: 1) the mutation causes local structural distortions, which result in changes in the shape of the ligand-binding pocket; 2) the mutation alters the dynamic nature of the protein and results in a more diverse conformational distribution of the ligand-binding pocket; and 3) the effect of the mutation on AR structure could be largely reversed by lowering the temperature at which the MD simulations were conducted. These results therefore strongly support the biochemical data, e.g., the mutants' inability to form AR-ligand complexes at 371C and its characteristic reversible thermolability, clearly indicating the value of such computational methods. Hum Mutat 22:465-475,
Human Mutation, 2004
The current version of the androgen receptor (AR) gene mutations database is described. The total... more The current version of the androgen receptor (AR) gene mutations database is described. The total number of reported mutations has risen from 374 to 605, and the number of AR-interacting proteins described has increased from 23 to 70, both over the past 3 years. A 3D model of the AR ligand-binding domain (AR LBD) has been added to give a better understanding of gene structure-function relationships. In addition, silent mutations have now been reported in both androgen insensitivity syndrome (AIS) and prostate cancer (CaP) cases. The database also now incorporates information on the exon 1 CAG repeat expansion disease, spinobulbar muscular atrophy (SBMA), as well as CAG repeat length variations associated with risk for female breast, uterine endometrial, colorectal, and prostate cancer, as well as for male infertility. The possible implications of somatic mutations, as opposed to germline mutations, in the development of future locus-specific mutation databases (LSDBs) is discussed. The database is available on the Internet (http://www.mcgill.ca/androgendb/).
Liver Transplantation, 2006
BACKGROUND: Hepatic ischemia/reperfusion (I/R) injury is characterized by the generation of react... more BACKGROUND: Hepatic ischemia/reperfusion (I/R) injury is characterized by the generation of reactive oxygen species (ROS), such as superoxide anions and hydrogen peroxide. The aim of this study is to investigate whether anti-oxidative gene delivery by our polylipid nanoparticles (PLNP) is an effective approach for prevention of the injury. METHODS: Polyplexes of extracellular superoxide dismutase (EC-SOD) and/or catalase genes were injected via the portal vein one day prior to a warm I/R procedure in mice. The effects of the gene delivery were determined 6 hours after starting reperfusion. RESULTS: PLNP-mediated anti-oxidative gene delivery led to a marked increase in human EC-SOD and catalase gene expression in the liver. Liver SOD and catalase activity both increased approximately 10 fold. Increased liver superoxide anion levels caused by the I/R procedure were reduced to normal levels by EC-SOD gene delivery. The overexpression of these two anti-oxidative genes significantly suppressed the I/R-induced elevation of serum alanine aminotransferase levels, decreased liver malondialdehyde content, restored glutathione reserve, and improved liver histology. CONCLUSION: EC-SOD or catalase gene delivery by PLNP resulted in high levels of the transgene activity in the liver, and markedly attenuated hepatic I/R injury. The protection is directly associated with elevated anti-oxidative enzyme activity as the result of the gene delivery. This novel approach may become a potential therapy to improve graft function and survival after liver transplantation.