Lucia Sivilotti | University College London (original) (raw)

Lucia Sivilotti

Because of the way Academia.edu is set up, this site is too much trouble to maintain- please go to my publications on
https://iris.ucl.ac.uk/iris/browse/profile?upi=LGSIV31
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Papers by Lucia Sivilotti

Research paper thumbnail of Recombinant nicotinic receptors, expressed in Xenopus oocytes, do not resemble native rat sympathetic ganglion receptors in single-channel behaviour

The Journal of Physiology, Apr 1, 1997

Research paper thumbnail of Single channel properties of glycine receptors from rat spinal motoneurones

Research paper thumbnail of Single Ion Channels

Research paper thumbnail of The startle disease mutation α1S270T predicts shortening of glycinergic synaptic currents

The Journal of Physiology, Jun 18, 2020

Research paper thumbnail of Properties of human glycine receptors containing the hyperekplexia mutation α1(K276E), expressed in<i>Xenopus</i>oocytes

The Journal of Physiology, Feb 1, 1998

Research paper thumbnail of Comparison of neuronal nicotinic receptors in rat sympathetic neurones with subunit pairs expressed in Xenopus oocytes

The Journal of Physiology, Nov 15, 1994

Research paper thumbnail of Agonist and blocking actions of choline and tetramethylammonium on human muscle acetylcholine receptors

The Journal of Physiology, Oct 30, 2009

Research paper thumbnail of Single-Channel Behavior of Heteromeric α1β Glycine Receptors: An Attempt to Detect a Conformational Change before the Channel Opens

The Journal of Neuroscience, Dec 1, 2004

Research paper thumbnail of The long activations of α2 glycine channels can be described by a mechanism with reaction intermediates (“flip”)

The Journal of General Physiology, Jan 31, 2011

Research paper thumbnail of Single-channel study of the spasmodic mutation α1A52S in recombinant rat glycine receptors

The Journal of Physiology, May 4, 2007

Research paper thumbnail of The α1K276E Startle Disease Mutation Reveals Multiple Intermediate States in the Gating of Glycine Receptors

The Journal of Neuroscience, Jan 25, 2012

Research paper thumbnail of Channel Blocking Properties Of Tetramethylammonium At The Human Muscle Acetylcholine Receptor

Biophysical Journal, Feb 1, 2009

Research paper thumbnail of Mechanism of activation of the prokaryotic channel ELIC by propylamine: A single-channel study

The Journal of General Physiology, Dec 29, 2014

Research paper thumbnail of Molecular Determinants in Glycine and GABA-A Receptors that Sense Intracellular Chloride Concentration

Biophysical Journal, 2010

Research paper thumbnail of The K276E Startle Disease Mutation in the Glycine Receptor: Effects on Channel Gating

Biophysical Journal, Feb 1, 2011

Research paper thumbnail of Acetylcholine Receptor-Nicotinic

Research paper thumbnail of Author response: Aminomethanesulfonic acid illuminates the boundary between full and partial agonists of the pentameric glycine receptor

Research paper thumbnail of Acidic pH reduces agonist efficacy and responses to synaptic‐like glycine applications in zebrafish α1 and rat α1β recombinant glycine receptors

The Journal of Physiology, 2021

Research paper thumbnail of The intracellular domain of homomeric glycine receptors modulates agonist efficacy

Journal of Biological Chemistry, 2020

Like other pentameric ligand-gated channels, glycine receptors (GlyRs) contain long intracellular... more Like other pentameric ligand-gated channels, glycine receptors (GlyRs) contain long intracellular domains (ICDs) between transmembrane helices 3 and 4. Structurally characterized GlyRs are generally engineered to have a very short ICD. We show here that for one such construct, zebrafish GlyREM, the agonists glycine, β-alanine, taurine, and GABA have high efficacy and produce maximum single-channel open probabilities greater than 0.9. In contrast, for full-length human α1 GlyR, taurine and GABA were clearly partial agonists, with maximum open probabilities of 0.46 and 0.09, respectively. We found that the elevated open probabilities in GlyREM are not due to the limited sequence differences between the human and zebrafish orthologs, but rather to replacement of the native ICD with a short tripeptide ICD. Consistent with this interpretation, shortening the ICD in the human GlyR increased the maximum open probability produced by taurine and GABA to 0.90 and 0.70, respectively, but furth...

Research paper thumbnail of Pentameric Ligand-Gated Ion Channel ELIC in Complex with Barium

Research paper thumbnail of Recombinant nicotinic receptors, expressed in Xenopus oocytes, do not resemble native rat sympathetic ganglion receptors in single-channel behaviour

The Journal of Physiology, Apr 1, 1997

Research paper thumbnail of Single channel properties of glycine receptors from rat spinal motoneurones

Research paper thumbnail of Single Ion Channels

Research paper thumbnail of The startle disease mutation α1S270T predicts shortening of glycinergic synaptic currents

The Journal of Physiology, Jun 18, 2020

Research paper thumbnail of Properties of human glycine receptors containing the hyperekplexia mutation α1(K276E), expressed in<i>Xenopus</i>oocytes

The Journal of Physiology, Feb 1, 1998

Research paper thumbnail of Comparison of neuronal nicotinic receptors in rat sympathetic neurones with subunit pairs expressed in Xenopus oocytes

The Journal of Physiology, Nov 15, 1994

Research paper thumbnail of Agonist and blocking actions of choline and tetramethylammonium on human muscle acetylcholine receptors

The Journal of Physiology, Oct 30, 2009

Research paper thumbnail of Single-Channel Behavior of Heteromeric α1β Glycine Receptors: An Attempt to Detect a Conformational Change before the Channel Opens

The Journal of Neuroscience, Dec 1, 2004

Research paper thumbnail of The long activations of α2 glycine channels can be described by a mechanism with reaction intermediates (“flip”)

The Journal of General Physiology, Jan 31, 2011

Research paper thumbnail of Single-channel study of the spasmodic mutation α1A52S in recombinant rat glycine receptors

The Journal of Physiology, May 4, 2007

Research paper thumbnail of The α1K276E Startle Disease Mutation Reveals Multiple Intermediate States in the Gating of Glycine Receptors

The Journal of Neuroscience, Jan 25, 2012

Research paper thumbnail of Channel Blocking Properties Of Tetramethylammonium At The Human Muscle Acetylcholine Receptor

Biophysical Journal, Feb 1, 2009

Research paper thumbnail of Mechanism of activation of the prokaryotic channel ELIC by propylamine: A single-channel study

The Journal of General Physiology, Dec 29, 2014

Research paper thumbnail of Molecular Determinants in Glycine and GABA-A Receptors that Sense Intracellular Chloride Concentration

Biophysical Journal, 2010

Research paper thumbnail of The K276E Startle Disease Mutation in the Glycine Receptor: Effects on Channel Gating

Biophysical Journal, Feb 1, 2011

Research paper thumbnail of Acetylcholine Receptor-Nicotinic

Research paper thumbnail of Author response: Aminomethanesulfonic acid illuminates the boundary between full and partial agonists of the pentameric glycine receptor

Research paper thumbnail of Acidic pH reduces agonist efficacy and responses to synaptic‐like glycine applications in zebrafish α1 and rat α1β recombinant glycine receptors

The Journal of Physiology, 2021

Research paper thumbnail of The intracellular domain of homomeric glycine receptors modulates agonist efficacy

Journal of Biological Chemistry, 2020

Like other pentameric ligand-gated channels, glycine receptors (GlyRs) contain long intracellular... more Like other pentameric ligand-gated channels, glycine receptors (GlyRs) contain long intracellular domains (ICDs) between transmembrane helices 3 and 4. Structurally characterized GlyRs are generally engineered to have a very short ICD. We show here that for one such construct, zebrafish GlyREM, the agonists glycine, β-alanine, taurine, and GABA have high efficacy and produce maximum single-channel open probabilities greater than 0.9. In contrast, for full-length human α1 GlyR, taurine and GABA were clearly partial agonists, with maximum open probabilities of 0.46 and 0.09, respectively. We found that the elevated open probabilities in GlyREM are not due to the limited sequence differences between the human and zebrafish orthologs, but rather to replacement of the native ICD with a short tripeptide ICD. Consistent with this interpretation, shortening the ICD in the human GlyR increased the maximum open probability produced by taurine and GABA to 0.90 and 0.70, respectively, but furth...

Research paper thumbnail of Pentameric Ligand-Gated Ion Channel ELIC in Complex with Barium

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