Jean-françois Rees | UCLouvain (University of Louvain) (original) (raw)

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Papers by Jean-françois Rees

Research paper thumbnail of Osmotic resistance as a quality diagnostic for penaeid postlarvae

Research paper thumbnail of Precision-Cut Liver Slices To Investigate Responsiveness of Deep-Sea Fish to Contaminants at High Pressure

Environmental Science & Technology, 2012

While deep-sea fish accumulate high levels of persistent organic pollutants (POPs), the toxicity ... more While deep-sea fish accumulate high levels of persistent organic pollutants (POPs), the toxicity associated with this contamination remains unknown. Indeed, the recurrent collection of moribund individuals precludes experimental studies to investigate POP effects in this fauna. We show that precision-cut liver slices (PCLS), an in vitro tool commonly used in human and rodent toxicology, can overcome such limitation. This technology was applied to individuals of the deep-sea grenadier Coryphaenoides rupestris directly upon retrieval from 530-m depth in Trondheimsfjord (Norway). PCLS remained viable and functional for 15 h when maintained in an appropriate culture media at 4 °C. This allowed experimental exposure of liver slices to the model POP 3-methylcholanthrene (3-MC; 25 μM) at levels of hydrostatic pressure mimicking shallow (0.1 megapascal or MPa) and deep-sea (5-15 MPa; representative of 500-1500 m depth) environments. As in shallow water fish, 3-MC induced the transcription of the detoxification enzyme cytochrome P4501A (CYP1A; a biomarker of exposure to POPs). This induction was diminished at elevated pressure, suggesting a limited responsiveness of C. rupestris toward POPs in its native environment. This very first in vitro toxicological investigation on a deep-sea fish opens the route for understanding pollutants effects in this highly exposed fauna.

Research paper thumbnail of Diet and weaning age affect growth and condition of Dover sole (Soleasolea L.)

Ciencias Marinas

Ciencias Marinas (2005), 31(3): 477-489 477 Diet and weaning age affect the growth and condition ... more Ciencias Marinas (2005), 31(3): 477-489 477 Diet and weaning age affect the growth and condition of Dover sole (Solea solea L.)

Research paper thumbnail of Metabolic control of spontaneous glowing in isolated Porichthys photophores

1. Isolated photophores of the epipelagic fish Porichthys notatus become spontaneously luminescen... more 1. Isolated photophores of the epipelagic fish Porichthys notatus become spontaneously luminescent after a few hours in saline at 20°C. This luminescence is not significantly affected by the adrenergic antagonists propranolol and phentolamine.

Research paper thumbnail of Nonspecific effects of propranolol on the photogenic cells of Porichthys isolated photophores

Comparative Biochemistry and Physiology Part C: Comparative Pharmacology, 1990

ABSTRACT

Research paper thumbnail of Purinergic receptors as a regulatory process of luminescence inPorichthys' luminous organs

Journal of Experimental Zoology, 1991

ABSTRACT

Research paper thumbnail of Effects of sodium azide on the Porichthys isolated luminous organs

Comparative Biochemistry and Physiology Part C: Comparative Pharmacology, 1990

... been proposed for the luminescent reaction of the Vargula system (Tsuji et al., 1977), which ... more ... been proposed for the luminescent reaction of the Vargula system (Tsuji et al., 1977), which is similar to the Porichthys photophore system (Cormier et al ... REFERENCES Alien JL and Sills JB (1973) Preparation and properties of quinaldine sulphate, an improved fish anaesthetic. ...

Research paper thumbnail of Metabolic control of spontaneous glowing in isolated photophores of Porichthys Peer reviewed article

1. Isolated photophores of the epipelagic fish Porichthys notatus become spontaneously luminescen... more 1. Isolated photophores of the epipelagic fish Porichthys notatus become spontaneously luminescent after a few hours in saline at 20°C. This luminescence is not significantly affected by the adrenergic antagonists propranolol and phentolamine.

Research paper thumbnail of Iodoacetic acid-induced luminescence of Porichthys isolated luminous organ Peer reviewed article

Research paper thumbnail of Glucose metabolism and luminescence control of Porichthys photophores Peer reviewed article

Research paper thumbnail of Effects of the β-adrenergic antagonist propranolol on Porichthys luminous organs

Research paper thumbnail of Iodoacetic Acid-induced Luminescence of Porichthys Isolated Luminous Organ

Research paper thumbnail of Glucose-metabolism and Luminescence Control of Porichthys Photophores

Research paper thumbnail of Metabolic control of luminescence in the luminous organs of the teleost Porichthys: effects of the metabolic inhibitors iodoacetic acid and potassium cyanide

Research paper thumbnail of Impacts of antibiotics on in vitro UVA-susceptibility of human skin fibroblasts

European journal of dermatology: EJD

Many studies of UVA-induced cell damage use skin cells obtained during plastic surgery. As the sk... more Many studies of UVA-induced cell damage use skin cells obtained during plastic surgery. As the skin is contaminated by micro-organisms, antibiotics need to be added to primary skin cell culture media. This study analysed the impact of the most widely used agents, penicillin, streptomycin, and amphotericin B deoxycholate (amB), on UVA-irradiated human skin fibroblasts. The results show that the presence of amB in cell culture media increases the susceptibility of fibroblasts to UVA and the intracellular level of reactive oxygen species, even when cells are irradiated in amB-free saline. This photosensitising effect of amB can be prevented if the antifungal agent is removed from the culture medium at least 24 hours before irradiation. Moreover, the use of streptomycin during cell culture partly protects cells against the UVA-induced mortality linked to amB. Acellular tests on lipid micelles suggest that this protective effect could result from an inhibition of lipid peroxidation by the antibacterial agent. In conclusion, antibiotics should be used with care in cell culture media if the cells are to be used in physiological studies of fine mechanisms in UVA-susceptibility of skin cells. In other cases, cells should be maintained in antibiotic-free media for 24 hours before irradiation.

Research paper thumbnail of Evidence for the utilization of coelenterazine as the luminescent substrate in Argyropelecus photophores

Molecular marine biology and biotechnology

Research paper thumbnail of Impacts of antibiotics on in vitro UVA-susceptibility of human skin fibroblasts

European journal of dermatology : EJD

Many studies of UVA-induced cell damage use skin cells obtained during plastic surgery. As the sk... more Many studies of UVA-induced cell damage use skin cells obtained during plastic surgery. As the skin is contaminated by micro-organisms, antibiotics need to be added to primary skin cell culture media. This study analysed the impact of the most widely used agents, penicillin, streptomycin, and amphotericin B deoxycholate (amB), on UVA-irradiated human skin fibroblasts. The results show that the presence of amB in cell culture media increases the susceptibility of fibroblasts to UVA and the intracellular level of reactive oxygen species, even when cells are irradiated in amB-free saline. This photosensitising effect of amB can be prevented if the antifungal agent is removed from the culture medium at least 24 hours before irradiation. Moreover, the use of streptomycin during cell culture partly protects cells against the UVA-induced mortality linked to amB. Acellular tests on lipid micelles suggest that this protective effect could result from an inhibition of lipid peroxidation by th...

Research paper thumbnail of Les luciférines d'organismes marins de profondeur: de nouveaux antioxydants très performants pour des applications thérapeutiques

Research paper thumbnail of What role for coelenterazine in the luminescent system of the hatchetfish Argyropelecus affinis?

Research paper thumbnail of Origins of luciferins: ecology of bioluminescence in marine fishes

Research paper thumbnail of Osmotic resistance as a quality diagnostic for penaeid postlarvae

Research paper thumbnail of Precision-Cut Liver Slices To Investigate Responsiveness of Deep-Sea Fish to Contaminants at High Pressure

Environmental Science & Technology, 2012

While deep-sea fish accumulate high levels of persistent organic pollutants (POPs), the toxicity ... more While deep-sea fish accumulate high levels of persistent organic pollutants (POPs), the toxicity associated with this contamination remains unknown. Indeed, the recurrent collection of moribund individuals precludes experimental studies to investigate POP effects in this fauna. We show that precision-cut liver slices (PCLS), an in vitro tool commonly used in human and rodent toxicology, can overcome such limitation. This technology was applied to individuals of the deep-sea grenadier Coryphaenoides rupestris directly upon retrieval from 530-m depth in Trondheimsfjord (Norway). PCLS remained viable and functional for 15 h when maintained in an appropriate culture media at 4 °C. This allowed experimental exposure of liver slices to the model POP 3-methylcholanthrene (3-MC; 25 μM) at levels of hydrostatic pressure mimicking shallow (0.1 megapascal or MPa) and deep-sea (5-15 MPa; representative of 500-1500 m depth) environments. As in shallow water fish, 3-MC induced the transcription of the detoxification enzyme cytochrome P4501A (CYP1A; a biomarker of exposure to POPs). This induction was diminished at elevated pressure, suggesting a limited responsiveness of C. rupestris toward POPs in its native environment. This very first in vitro toxicological investigation on a deep-sea fish opens the route for understanding pollutants effects in this highly exposed fauna.

Research paper thumbnail of Diet and weaning age affect growth and condition of Dover sole (Soleasolea L.)

Ciencias Marinas

Ciencias Marinas (2005), 31(3): 477-489 477 Diet and weaning age affect the growth and condition ... more Ciencias Marinas (2005), 31(3): 477-489 477 Diet and weaning age affect the growth and condition of Dover sole (Solea solea L.)

Research paper thumbnail of Metabolic control of spontaneous glowing in isolated Porichthys photophores

1. Isolated photophores of the epipelagic fish Porichthys notatus become spontaneously luminescen... more 1. Isolated photophores of the epipelagic fish Porichthys notatus become spontaneously luminescent after a few hours in saline at 20°C. This luminescence is not significantly affected by the adrenergic antagonists propranolol and phentolamine.

Research paper thumbnail of Nonspecific effects of propranolol on the photogenic cells of Porichthys isolated photophores

Comparative Biochemistry and Physiology Part C: Comparative Pharmacology, 1990

ABSTRACT

Research paper thumbnail of Purinergic receptors as a regulatory process of luminescence inPorichthys' luminous organs

Journal of Experimental Zoology, 1991

ABSTRACT

Research paper thumbnail of Effects of sodium azide on the Porichthys isolated luminous organs

Comparative Biochemistry and Physiology Part C: Comparative Pharmacology, 1990

... been proposed for the luminescent reaction of the Vargula system (Tsuji et al., 1977), which ... more ... been proposed for the luminescent reaction of the Vargula system (Tsuji et al., 1977), which is similar to the Porichthys photophore system (Cormier et al ... REFERENCES Alien JL and Sills JB (1973) Preparation and properties of quinaldine sulphate, an improved fish anaesthetic. ...

Research paper thumbnail of Metabolic control of spontaneous glowing in isolated photophores of Porichthys Peer reviewed article

1. Isolated photophores of the epipelagic fish Porichthys notatus become spontaneously luminescen... more 1. Isolated photophores of the epipelagic fish Porichthys notatus become spontaneously luminescent after a few hours in saline at 20°C. This luminescence is not significantly affected by the adrenergic antagonists propranolol and phentolamine.

Research paper thumbnail of Iodoacetic acid-induced luminescence of Porichthys isolated luminous organ Peer reviewed article

Research paper thumbnail of Glucose metabolism and luminescence control of Porichthys photophores Peer reviewed article

Research paper thumbnail of Effects of the β-adrenergic antagonist propranolol on Porichthys luminous organs

Research paper thumbnail of Iodoacetic Acid-induced Luminescence of Porichthys Isolated Luminous Organ

Research paper thumbnail of Glucose-metabolism and Luminescence Control of Porichthys Photophores

Research paper thumbnail of Metabolic control of luminescence in the luminous organs of the teleost Porichthys: effects of the metabolic inhibitors iodoacetic acid and potassium cyanide

Research paper thumbnail of Impacts of antibiotics on in vitro UVA-susceptibility of human skin fibroblasts

European journal of dermatology: EJD

Many studies of UVA-induced cell damage use skin cells obtained during plastic surgery. As the sk... more Many studies of UVA-induced cell damage use skin cells obtained during plastic surgery. As the skin is contaminated by micro-organisms, antibiotics need to be added to primary skin cell culture media. This study analysed the impact of the most widely used agents, penicillin, streptomycin, and amphotericin B deoxycholate (amB), on UVA-irradiated human skin fibroblasts. The results show that the presence of amB in cell culture media increases the susceptibility of fibroblasts to UVA and the intracellular level of reactive oxygen species, even when cells are irradiated in amB-free saline. This photosensitising effect of amB can be prevented if the antifungal agent is removed from the culture medium at least 24 hours before irradiation. Moreover, the use of streptomycin during cell culture partly protects cells against the UVA-induced mortality linked to amB. Acellular tests on lipid micelles suggest that this protective effect could result from an inhibition of lipid peroxidation by the antibacterial agent. In conclusion, antibiotics should be used with care in cell culture media if the cells are to be used in physiological studies of fine mechanisms in UVA-susceptibility of skin cells. In other cases, cells should be maintained in antibiotic-free media for 24 hours before irradiation.

Research paper thumbnail of Evidence for the utilization of coelenterazine as the luminescent substrate in Argyropelecus photophores

Molecular marine biology and biotechnology

Research paper thumbnail of Impacts of antibiotics on in vitro UVA-susceptibility of human skin fibroblasts

European journal of dermatology : EJD

Many studies of UVA-induced cell damage use skin cells obtained during plastic surgery. As the sk... more Many studies of UVA-induced cell damage use skin cells obtained during plastic surgery. As the skin is contaminated by micro-organisms, antibiotics need to be added to primary skin cell culture media. This study analysed the impact of the most widely used agents, penicillin, streptomycin, and amphotericin B deoxycholate (amB), on UVA-irradiated human skin fibroblasts. The results show that the presence of amB in cell culture media increases the susceptibility of fibroblasts to UVA and the intracellular level of reactive oxygen species, even when cells are irradiated in amB-free saline. This photosensitising effect of amB can be prevented if the antifungal agent is removed from the culture medium at least 24 hours before irradiation. Moreover, the use of streptomycin during cell culture partly protects cells against the UVA-induced mortality linked to amB. Acellular tests on lipid micelles suggest that this protective effect could result from an inhibition of lipid peroxidation by th...

Research paper thumbnail of Les luciférines d'organismes marins de profondeur: de nouveaux antioxydants très performants pour des applications thérapeutiques

Research paper thumbnail of What role for coelenterazine in the luminescent system of the hatchetfish Argyropelecus affinis?

Research paper thumbnail of Origins of luciferins: ecology of bioluminescence in marine fishes