Jose Luis Prieto | Universidad Complutense de Madrid (original) (raw)
Papers by Jose Luis Prieto
The American Journal on Addictions, 2015
Caffeine is an active adulterant found in several drugs of abuse including coca paste (CP). We ha... more Caffeine is an active adulterant found in several drugs of abuse including coca paste (CP). We had previously demonstrated that caffeine potentiated the acute stimulant effect induced by CP seized samples. The role of caffeine in the expression of sensitization elicited by a CP seized sample (CP1) was here evaluated. CP1 (equivalent dose of 10 mg/kg of cocaine), cocaine (pure, 10 mg/kg), a combination of cocaine 10 mg/kg plus caffeine 2.5 mg/kg (CP1-surrogate) and saline (control) were intraperitoneally injected in male rats under two different sensitization schedules. Ambulatory locomotion was recorded in 58 animals. After five daily CP1 injections and 5 days of withdrawal, CP1-challenged animals displayed a more robust sensitization than cocaine-treated animals. When a 3 injections-regime of CP1-surrogate or cocaine was assayed, only CP1-surrogate was able to elicit sensitization. Caffeine enhances and accelerates the CP1-induced sensitization. Results may shed light on the fast and high dependence observed in CP users. (Am J Addict 2015;XX:XX -XX).
Medicina oral, patología oral y cirugía bucal
The infection of the oral cavity is a common public health problem and constant cause for antibio... more The infection of the oral cavity is a common public health problem and constant cause for antibiotic prescription, with 10% of antibiotics used to treat this problem. However, few studies have so far aimed to determine its incidence. Added to this, its relationship with certain sytemic diseases (cardiac, endocrine, etc) confers this pathology vital importance. In spite of the frequency and importance of odontogenic infection, the current dispersion in criteria regarding key aspects in classification, terminology and therapeutic recommendations is noticeable. The main objective of this document, compiled as a consensus statement by specialists in microbiology and odontology, is to establish useful recommendations for all of those involved in the clinical management of this pathology. Special attention has been placed on the rise in bacterial resistance observed over the last years, specifically the proliferation of betalactamase producing strains. Another important factor causing the...
Objectives: To evaluate the value of plasma fibronectin (pFN) as a diagnostic marker of sepsis.
La enfermedad pulmonar obstructiva crónica (EPOC) constituye un problema de salud pública de gran... more La enfermedad pulmonar obstructiva crónica (EPOC) constituye un problema de salud pública de gran importancia; tiene característicamente un curso crónico y progresivo con exacerbaciones y puede conducir a la incapacidad y a la muerte. Representa una causa frecuente de muerte en nuestro país, tanto en varones como en mujeres, con una mortalidad nada desdeñable 1 .
British Journal of Infection Control, 2007
The need for evidence-based infection prevention and control practice necessitates ensuring resea... more The need for evidence-based infection prevention and control practice necessitates ensuring research capacity and capability to deliver this agenda. This paper reports the findings of a research mapping exercise to identify current research activity and perceived need for support among members of the ICNA. These findings provide insights into how the ICNA research and development group can support practitioners in
Farmaco (Società chimica italiana : 1989), 2003
The angiotensin-converting enzyme inhibitors cilazapril, quinapril and ramipril are reduced at a ... more The angiotensin-converting enzyme inhibitors cilazapril, quinapril and ramipril are reduced at a hanging mercury drop electrode in the pH range 3.5-13 using Britton-Robinson buffers as supporting electrolyte and KCl as ionic medium. Square wave voltammetry has proved to be the most suitable electroanalytical technique for the quantitative voltammetric determination of these antihypertensive drugs. Optimisation of the chemical and instrumental variables was carried out. Analyses were performed in 0.02 M borate buffer at pH 9.5 and 0.5 M KCl as ionic medium, using a pulse amplitude of 50 mV and a frequency of 150 Hz. A linear relationship between peak current and concentration was found in the interval 0.5-8 microg/ml for cilazapril and up to 6 microg/ml for quinapril and ramipril, allowing the direct determination of their pharmaceutical formulations alone or mixed with hydrochlorothiazide. Good accuracy and repeatativity were obtained.
Journal of forensic sciences, 2004
Estimating time since death is especially difficult in the examination of poorly preserved cadave... more Estimating time since death is especially difficult in the examination of poorly preserved cadavers and depends on the experience of the examiner and comparison with previously documented cases showing similar characteristics. The present study reports on information obtained over the past ten years through the work of the Laboratorio de Antropología y Odontología Forense (LAF) of the Instituto Anatómico Forense de Madrid, Spain, in particular evaluating how the type of fracture influences postmortem change. From the original 225 forensic cases examined between 1992 and 2002 in the LAF, a sample of 29 cases were selected from various regions of the Spanish mainland. A data collection protocol was established to reflect factors which the existing specialized literature, documenting the relation existing in the sample analyzed between time since death and the extent of postmortem change, which in the environments examined are distributed into the following phases: Phase 1 (putrefactio...
Japanese Journal of Applied Physics, 1997
... June 1997 Spectroscopy, Imaging and Switching Behaviour of Individual InP/GalnP Quantum Dots ... more ... June 1997 Spectroscopy, Imaging and Switching Behaviour of Individual InP/GalnP Quantum Dots Pedro Castrillo*1, Dan Hessman, Mats-Erik Pistol, Jose Antonio Prieto*2 ... We thank W. Seifert and N. Carlsson for the growth of the sample and B. Junno for technical assistance. ...
Clinical biochemistry, 2009
Evaluation of a GC-MS method using N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA)... more Evaluation of a GC-MS method using N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA) as the silylating agent for GC-MS. Study of the stability of creatine and guanidinoacetate in urine. 22 urines were kept at RT, 4 degrees C and -30 degrees C for 15 days. MTBSTFA produces a single chromatographic peak in contrast with other derivatizing agents. Creatine concentration increases at room temperature (326% on average), and at 4 degrees C (75%). However, detection decreases after freezing (-37%). Guanidinoacetate is stable, but decreases after freezing (-37%). Sonication before analysis is crucial to obtain repetitive results. A modified GC-MS method has been validated and the conditions for preservation of the urine have been established.
Journal of plant research, 2013
Recent phylogenetic studies have shown that Saxifraga, as currently understood, must be divided i... more Recent phylogenetic studies have shown that Saxifraga, as currently understood, must be divided into two genera: Saxifraga L. sensu stricto and Micranthes Haw. To better understand the evolutionary history of these two genera, we performed phylogenetic analyses inferred from the nuclear ribosomal sequences from the internal transcribed spacer and the sequences of the plastid DNA (rbcL). Our molecular data confirmed the monophyly of the genus Micranthes and the consistency of the existing systematic treatments based on morphological criteria. Moreover, Micranthes species native from the Iberian Peninsula (i.e. M. clusii, M. lepismigena and M. stellaris) should be included into Micranthes sect. Arabisa.
The Biochemical journal, Jan 2, 2015
Glc7 is the only catalytic subunit of the protein phosphatase type 1 in the yeast S. cerevisiae a... more Glc7 is the only catalytic subunit of the protein phosphatase type 1 in the yeast S. cerevisiae and, together with its regulatory subunits, is involved in many and essential processes. Analysis of the non-essential mutants in the regulatory subunits of Glc7 revealed that the lack of Reg1, and no other subunit, causes hypersensitivity to unfolded-protein-response (UPR)-inducers, which was concomitant with an augmented UPR element-dependent transcriptional response. Glc7-Reg1complex takes part in the regulation of the yeast AMP-activated serine/threonine protein kinase Snf1 in response to glucose. We demonstrate here that the observed phenotypes of reg1 mutant cells are attributable to the inappropriate activation of Snf1. Indeed, growth in the presence of limited concentrations of glucose, where Snf1 is active, or expression of active forms of Snf1 in a wild-type strain, increased the sensitivity to the UPR-inducer tunicamycin. Furthermore, reg1 mutant cells showed a sustained HAC1 m...
Journal of Infection Prevention, 2009
A key objective in the IPS research and development strategy is to increase research capability a... more A key objective in the IPS research and development strategy is to increase research capability and capacity among the Society’s membership. This article summarises the recent work of the IPS Research and Development Group. On the fiftieth anniversary of the appointment of the first infection control nurse the IPS are launching annual research awards to encourage research active practitioners to
Enfermedades infecciosas y microbiología clínica, 2002
British Journal of Infection Control, 2005
he Research and Development Group was reestablished in October 2004 to advance the Infection Cont... more he Research and Development Group was reestablished in October 2004 to advance the Infection Control Nurses Association's research aspirations. This strategy is the result of the work of the group since its first meeting in January 2005. The draft strategy was discussed and approved by branch representatives and the National Executive Committee in June 2005. It sets out the background
Pediatric Research, 2008
Cardiovascular disease is an important cause of morbidity in recipients of renal transplants. The... more Cardiovascular disease is an important cause of morbidity in recipients of renal transplants. The aim of the present study was to analyze the status of the arginine-creatine pathway in such patients, given the relationship between the arginine metabolism and both renal function and the methionine-homocysteine cycle. Twentynine children and adolescents (median age 13, range 6 -18 years), who had received a renal allograft 14.5-82.0 months before, were recruited for the study. On immunosuppressive therapy, all patients evidenced an adequate level of renal function. Plasma concentrations of homocysteine and glycine were significantly higher, whereas urinary excretions of guanidinoacetate and creatine were significantly lower than controls. Urinary excretions of guanidinoacetate and creatine correlated positively with creatinine clearance. Urinary excretion of creatine was negatively correlated with plasma concentration of homocysteine. The demonstration of disturbances in the arginine-creatine pathway in patients with well-functioning renal transplants and in absence of chronic renal failure represents a novel finding. We speculate that the low urinary excretion of guanidinoacetate and creatine is probably related to the nephrotoxic effect of immunosuppressive therapy and to defective methylation associated with the presence of hyperhomocysteinemia. (Pediatr Res 64: 218-222, 2008)
Clinical and Experimental Allergy, 1999
Background The alveolar macrophage (AM) constitutes an important link between pulmonary innate an... more Background The alveolar macrophage (AM) constitutes an important link between pulmonary innate and adaptive immunity due to its antigen-presenting capacity and ability to express different immunomodulating mediators. The role of AMs in the pathogenesis of allergic in¯ammation has yet to be fully determined. Objective To investigate clinical effects and any change in the AM phenotype pattern after inhalation of sub-clinical doses of allergen by asthmatic patients. Methods Eight subjects with allergic asthma underwent repeated low-dose allergen provocations equivalent to 10% of PD 20 . AMs recovered with bronchoalveolar lavage (BAL) were characterized by¯ow cytometric analysis of adhesion molecules, co-stimulatory molecules and markers for AM population activation and heterogeneity. Results An allergic airway in¯ammation, sub-clinical in six out of eight subjects, was obtained after low-dose allergen provocations, as determined by increased airway methacholine reactivity, increased BAL¯uid total cell and eosinophil counts and increased serum ECP levels. The AMs showed a post-challenge altered phenotype pattern with a decreased expression of CD11a, CD16, CD71 and HLA class I and an increased expression of CD11b and CD14. The AMs were positive for CD83 and a weak post-challenge increase in the CD83 expression was found. Conclusion Repeated low-dose allergen exposure induces an allergic airway in¯ammation in asthmatic subjects. The in¯ammation is associated with an altered AM phenotype pattern, consistent with an in¯ux of monocytes and a hypothetical increased accessory cell function in the airways, possibly contributing to the development and sustenance of airway in¯ammation in asthma.
BACKGROUND: We report the outcome of debridement and prosthesis retention plus long-term levo- fl... more BACKGROUND: We report the outcome of debridement and prosthesis retention plus long-term levo- floxacin/rifampicin treatment of prosthetic joint infections. METHODS: Staphylococcal prosthesis joint infections were defined by positive culture of joint aspirate, intraoperative debridement specimens, or sinus tract discharge in the presence of clinical criteria. Patients received long-term oral levofloxacin 500 mg and rifampicin 600 mg once per day. Sixty
Physical Review B, 2004
The transport properties of sharp antiferromagnetic boundaries between Gd and Fe have been studie... more The transport properties of sharp antiferromagnetic boundaries between Gd and Fe have been studied. The measurements were carried out with the current perpendicular to the plane at 20 K and up to 8 T in multilayer Gd/Fe of different thicknesses. We have extended the Valet ...
The American Journal on Addictions, 2015
Caffeine is an active adulterant found in several drugs of abuse including coca paste (CP). We ha... more Caffeine is an active adulterant found in several drugs of abuse including coca paste (CP). We had previously demonstrated that caffeine potentiated the acute stimulant effect induced by CP seized samples. The role of caffeine in the expression of sensitization elicited by a CP seized sample (CP1) was here evaluated. CP1 (equivalent dose of 10 mg/kg of cocaine), cocaine (pure, 10 mg/kg), a combination of cocaine 10 mg/kg plus caffeine 2.5 mg/kg (CP1-surrogate) and saline (control) were intraperitoneally injected in male rats under two different sensitization schedules. Ambulatory locomotion was recorded in 58 animals. After five daily CP1 injections and 5 days of withdrawal, CP1-challenged animals displayed a more robust sensitization than cocaine-treated animals. When a 3 injections-regime of CP1-surrogate or cocaine was assayed, only CP1-surrogate was able to elicit sensitization. Caffeine enhances and accelerates the CP1-induced sensitization. Results may shed light on the fast and high dependence observed in CP users. (Am J Addict 2015;XX:XX -XX).
Medicina oral, patología oral y cirugía bucal
The infection of the oral cavity is a common public health problem and constant cause for antibio... more The infection of the oral cavity is a common public health problem and constant cause for antibiotic prescription, with 10% of antibiotics used to treat this problem. However, few studies have so far aimed to determine its incidence. Added to this, its relationship with certain sytemic diseases (cardiac, endocrine, etc) confers this pathology vital importance. In spite of the frequency and importance of odontogenic infection, the current dispersion in criteria regarding key aspects in classification, terminology and therapeutic recommendations is noticeable. The main objective of this document, compiled as a consensus statement by specialists in microbiology and odontology, is to establish useful recommendations for all of those involved in the clinical management of this pathology. Special attention has been placed on the rise in bacterial resistance observed over the last years, specifically the proliferation of betalactamase producing strains. Another important factor causing the...
Objectives: To evaluate the value of plasma fibronectin (pFN) as a diagnostic marker of sepsis.
La enfermedad pulmonar obstructiva crónica (EPOC) constituye un problema de salud pública de gran... more La enfermedad pulmonar obstructiva crónica (EPOC) constituye un problema de salud pública de gran importancia; tiene característicamente un curso crónico y progresivo con exacerbaciones y puede conducir a la incapacidad y a la muerte. Representa una causa frecuente de muerte en nuestro país, tanto en varones como en mujeres, con una mortalidad nada desdeñable 1 .
British Journal of Infection Control, 2007
The need for evidence-based infection prevention and control practice necessitates ensuring resea... more The need for evidence-based infection prevention and control practice necessitates ensuring research capacity and capability to deliver this agenda. This paper reports the findings of a research mapping exercise to identify current research activity and perceived need for support among members of the ICNA. These findings provide insights into how the ICNA research and development group can support practitioners in
Farmaco (Società chimica italiana : 1989), 2003
The angiotensin-converting enzyme inhibitors cilazapril, quinapril and ramipril are reduced at a ... more The angiotensin-converting enzyme inhibitors cilazapril, quinapril and ramipril are reduced at a hanging mercury drop electrode in the pH range 3.5-13 using Britton-Robinson buffers as supporting electrolyte and KCl as ionic medium. Square wave voltammetry has proved to be the most suitable electroanalytical technique for the quantitative voltammetric determination of these antihypertensive drugs. Optimisation of the chemical and instrumental variables was carried out. Analyses were performed in 0.02 M borate buffer at pH 9.5 and 0.5 M KCl as ionic medium, using a pulse amplitude of 50 mV and a frequency of 150 Hz. A linear relationship between peak current and concentration was found in the interval 0.5-8 microg/ml for cilazapril and up to 6 microg/ml for quinapril and ramipril, allowing the direct determination of their pharmaceutical formulations alone or mixed with hydrochlorothiazide. Good accuracy and repeatativity were obtained.
Journal of forensic sciences, 2004
Estimating time since death is especially difficult in the examination of poorly preserved cadave... more Estimating time since death is especially difficult in the examination of poorly preserved cadavers and depends on the experience of the examiner and comparison with previously documented cases showing similar characteristics. The present study reports on information obtained over the past ten years through the work of the Laboratorio de Antropología y Odontología Forense (LAF) of the Instituto Anatómico Forense de Madrid, Spain, in particular evaluating how the type of fracture influences postmortem change. From the original 225 forensic cases examined between 1992 and 2002 in the LAF, a sample of 29 cases were selected from various regions of the Spanish mainland. A data collection protocol was established to reflect factors which the existing specialized literature, documenting the relation existing in the sample analyzed between time since death and the extent of postmortem change, which in the environments examined are distributed into the following phases: Phase 1 (putrefactio...
Japanese Journal of Applied Physics, 1997
... June 1997 Spectroscopy, Imaging and Switching Behaviour of Individual InP/GalnP Quantum Dots ... more ... June 1997 Spectroscopy, Imaging and Switching Behaviour of Individual InP/GalnP Quantum Dots Pedro Castrillo*1, Dan Hessman, Mats-Erik Pistol, Jose Antonio Prieto*2 ... We thank W. Seifert and N. Carlsson for the growth of the sample and B. Junno for technical assistance. ...
Clinical biochemistry, 2009
Evaluation of a GC-MS method using N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA)... more Evaluation of a GC-MS method using N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA) as the silylating agent for GC-MS. Study of the stability of creatine and guanidinoacetate in urine. 22 urines were kept at RT, 4 degrees C and -30 degrees C for 15 days. MTBSTFA produces a single chromatographic peak in contrast with other derivatizing agents. Creatine concentration increases at room temperature (326% on average), and at 4 degrees C (75%). However, detection decreases after freezing (-37%). Guanidinoacetate is stable, but decreases after freezing (-37%). Sonication before analysis is crucial to obtain repetitive results. A modified GC-MS method has been validated and the conditions for preservation of the urine have been established.
Journal of plant research, 2013
Recent phylogenetic studies have shown that Saxifraga, as currently understood, must be divided i... more Recent phylogenetic studies have shown that Saxifraga, as currently understood, must be divided into two genera: Saxifraga L. sensu stricto and Micranthes Haw. To better understand the evolutionary history of these two genera, we performed phylogenetic analyses inferred from the nuclear ribosomal sequences from the internal transcribed spacer and the sequences of the plastid DNA (rbcL). Our molecular data confirmed the monophyly of the genus Micranthes and the consistency of the existing systematic treatments based on morphological criteria. Moreover, Micranthes species native from the Iberian Peninsula (i.e. M. clusii, M. lepismigena and M. stellaris) should be included into Micranthes sect. Arabisa.
The Biochemical journal, Jan 2, 2015
Glc7 is the only catalytic subunit of the protein phosphatase type 1 in the yeast S. cerevisiae a... more Glc7 is the only catalytic subunit of the protein phosphatase type 1 in the yeast S. cerevisiae and, together with its regulatory subunits, is involved in many and essential processes. Analysis of the non-essential mutants in the regulatory subunits of Glc7 revealed that the lack of Reg1, and no other subunit, causes hypersensitivity to unfolded-protein-response (UPR)-inducers, which was concomitant with an augmented UPR element-dependent transcriptional response. Glc7-Reg1complex takes part in the regulation of the yeast AMP-activated serine/threonine protein kinase Snf1 in response to glucose. We demonstrate here that the observed phenotypes of reg1 mutant cells are attributable to the inappropriate activation of Snf1. Indeed, growth in the presence of limited concentrations of glucose, where Snf1 is active, or expression of active forms of Snf1 in a wild-type strain, increased the sensitivity to the UPR-inducer tunicamycin. Furthermore, reg1 mutant cells showed a sustained HAC1 m...
Journal of Infection Prevention, 2009
A key objective in the IPS research and development strategy is to increase research capability a... more A key objective in the IPS research and development strategy is to increase research capability and capacity among the Society’s membership. This article summarises the recent work of the IPS Research and Development Group. On the fiftieth anniversary of the appointment of the first infection control nurse the IPS are launching annual research awards to encourage research active practitioners to
Enfermedades infecciosas y microbiología clínica, 2002
British Journal of Infection Control, 2005
he Research and Development Group was reestablished in October 2004 to advance the Infection Cont... more he Research and Development Group was reestablished in October 2004 to advance the Infection Control Nurses Association's research aspirations. This strategy is the result of the work of the group since its first meeting in January 2005. The draft strategy was discussed and approved by branch representatives and the National Executive Committee in June 2005. It sets out the background
Pediatric Research, 2008
Cardiovascular disease is an important cause of morbidity in recipients of renal transplants. The... more Cardiovascular disease is an important cause of morbidity in recipients of renal transplants. The aim of the present study was to analyze the status of the arginine-creatine pathway in such patients, given the relationship between the arginine metabolism and both renal function and the methionine-homocysteine cycle. Twentynine children and adolescents (median age 13, range 6 -18 years), who had received a renal allograft 14.5-82.0 months before, were recruited for the study. On immunosuppressive therapy, all patients evidenced an adequate level of renal function. Plasma concentrations of homocysteine and glycine were significantly higher, whereas urinary excretions of guanidinoacetate and creatine were significantly lower than controls. Urinary excretions of guanidinoacetate and creatine correlated positively with creatinine clearance. Urinary excretion of creatine was negatively correlated with plasma concentration of homocysteine. The demonstration of disturbances in the arginine-creatine pathway in patients with well-functioning renal transplants and in absence of chronic renal failure represents a novel finding. We speculate that the low urinary excretion of guanidinoacetate and creatine is probably related to the nephrotoxic effect of immunosuppressive therapy and to defective methylation associated with the presence of hyperhomocysteinemia. (Pediatr Res 64: 218-222, 2008)
Clinical and Experimental Allergy, 1999
Background The alveolar macrophage (AM) constitutes an important link between pulmonary innate an... more Background The alveolar macrophage (AM) constitutes an important link between pulmonary innate and adaptive immunity due to its antigen-presenting capacity and ability to express different immunomodulating mediators. The role of AMs in the pathogenesis of allergic in¯ammation has yet to be fully determined. Objective To investigate clinical effects and any change in the AM phenotype pattern after inhalation of sub-clinical doses of allergen by asthmatic patients. Methods Eight subjects with allergic asthma underwent repeated low-dose allergen provocations equivalent to 10% of PD 20 . AMs recovered with bronchoalveolar lavage (BAL) were characterized by¯ow cytometric analysis of adhesion molecules, co-stimulatory molecules and markers for AM population activation and heterogeneity. Results An allergic airway in¯ammation, sub-clinical in six out of eight subjects, was obtained after low-dose allergen provocations, as determined by increased airway methacholine reactivity, increased BAL¯uid total cell and eosinophil counts and increased serum ECP levels. The AMs showed a post-challenge altered phenotype pattern with a decreased expression of CD11a, CD16, CD71 and HLA class I and an increased expression of CD11b and CD14. The AMs were positive for CD83 and a weak post-challenge increase in the CD83 expression was found. Conclusion Repeated low-dose allergen exposure induces an allergic airway in¯ammation in asthmatic subjects. The in¯ammation is associated with an altered AM phenotype pattern, consistent with an in¯ux of monocytes and a hypothetical increased accessory cell function in the airways, possibly contributing to the development and sustenance of airway in¯ammation in asthma.
BACKGROUND: We report the outcome of debridement and prosthesis retention plus long-term levo- fl... more BACKGROUND: We report the outcome of debridement and prosthesis retention plus long-term levo- floxacin/rifampicin treatment of prosthetic joint infections. METHODS: Staphylococcal prosthesis joint infections were defined by positive culture of joint aspirate, intraoperative debridement specimens, or sinus tract discharge in the presence of clinical criteria. Patients received long-term oral levofloxacin 500 mg and rifampicin 600 mg once per day. Sixty
Physical Review B, 2004
The transport properties of sharp antiferromagnetic boundaries between Gd and Fe have been studie... more The transport properties of sharp antiferromagnetic boundaries between Gd and Fe have been studied. The measurements were carried out with the current perpendicular to the plane at 20 K and up to 8 T in multilayer Gd/Fe of different thicknesses. We have extended the Valet ...