Bertil Damato | University of California, San Francisco (original) (raw)

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Papers by Bertil Damato

Acta Ophthalmologica Scandinavica, 2007

... 8 Dept of Ophthalmology, Croix-Rousse Hospital and Centre Léon Bérard, Lyon. 9 ... Cancer 200... more ... 8 Dept of Ophthalmology, Croix-Rousse Hospital and Centre Léon Bérard, Lyon. 9 ... Cancer 2003; 97: 6575). Patients whose predicted survival was less than 6 months were assigned to stage IVc, between 6 and 12 months to stage IVb and over 12 months to stage IVa. ...

Investigative Ophthalmology & Visual Science, 2010

Journal of Clinical Oncology, 2013

Acta Ophthalmologica, 2013

ABSTRACT Purpose To identify potential therapeutic targets in uveal melanoma by global proteomic ... more ABSTRACT Purpose To identify potential therapeutic targets in uveal melanoma by global proteomic analysis of tumours with high- (HR) or low- (LR) risk of developing metastatic disease. Methods Proteins were extracted from fresh-frozen tissues from 10 HR and 10 LR UM and from 22 normal choroid samples, subjected to isobaric tagging for relative quantification (iTRAQ) and analysed by nanoLC-MS/MS. Peptide identification was performed using Protein Pilot with a False Discovery Rate set at 5%. Differential expression of proteins and their relative abundance between the groups was investigated by supervised and unsupervised hierarchical clustering and outlier analyses. Results We identified 2651 unique proteins. Compared to normal choroid, the tumours showed 672 proteins up-regulated more than 1.5-fold and 459 down-regulated more than 1.5-fold. Of these, 18 proteins with known functions in tumour development/progression were identified and shown to be differentially expressed between HR and LR samples. Four of these have been further validated by immunohistochemistry in a larger cohort of patient samples. Conclusion Using quantitative proteomic analysis and immunohistochemistry we have identified novel potential therapeutic targets in UM. One of these in particular is a tumour suppressor associated with chemosensitivity in other cancers, but not yet described in UM. This opens a novel therapeutic avenue that is being further investigated.

Acta Ophthalmologica, 2011

ABSTRACT Purpose Intratumour heterogeneity of uveal melanoma (UM) has become a relevant issue in ... more ABSTRACT Purpose Intratumour heterogeneity of uveal melanoma (UM) has become a relevant issue in the era of sampling for prognostication purposes. We present the case of a UM consisting of two very distinct components.Methods A 53-year-old man was referred for an infero-nasal, collar-stud UM with associated retinal detachment in the right eye. On ultrasonography, the tumour measured 14 x 16mm, with a thickness of 11mm. The patient chose enucleation over other treatment options. The eye was examined morphologically, immunohistochemically and using multiplex ligation-dependent probe amplification (MLPA).Results Histological sections demonstrated two distinct components: a pale basal part consisting of amelanotic epithelioid melanoma cells with extensive diffuse involvement of the ocular structures. In contrast, the apical part was heavily pigmented comprised only of spindle B cells. Interestingly, MLPA demonstrated complete chromosome 3 loss in the apical (spindle) region but only partial chromosome 3 deletion in the basal (epithelioid) part. Both areas showed polysomy 8. Conversely, immunohistochemistry showed more aggressive features in the basal part rather than in the apical part.Conclusion This case provides further evidence that there is heterogeneity in UM, and that a single intraocular biopsy may not be reliable for prognostication purposes.

The American Journal of Pathology, 2013